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Human hemin

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https://www.readbyqxmd.com/read/29311283/the-corynebacterium-diphtheriae-iron-regulated-surface-protein-hbpa-is-involved-in-the-utilization-of-the-hemoglobin-haptoglobin-complex-as-an-iron-source
#1
Lindsey R Lyman, Eric D Peng, Michael P Schmitt
Corynebacterium diphtheriae utilizes various heme-containing proteins, including hemoglobin (Hb) and the hemoglobin-haptoglobin complex (Hb-Hp), as iron sources during growth in iron-depleted environments. The ability to utilize Hb-Hp as an iron source requires the surface anchored proteins HtaA and either ChtA or ChtC. The ability to bind hemin, Hb and Hb-Hp by each of these C. diphtheriae proteins requires the previously characterized Conserved Region (CR) domain. In this study, we identified an Hb-Hp binding protein, HbpA (38...
January 8, 2018: Journal of Bacteriology
https://www.readbyqxmd.com/read/29299545/colorimetric-molecular-diagnosis-of-the-hiv-gag-gene-using-dnazyme-and-a-complementary-dna-extended-primer
#2
Seong U Kim, Bhagwan S Batule, Hyoyoung Mun, Ju-Young Byun, Won-Bo Shim, Min-Gon Kim
We have developed a novel strategy for the colorimetric detection of PCR products by utilizing a target-specific primer modified at the 5'-end with an anti-DNAzyme sequence. A single-stranded DNAzyme sequence folds into a G-quadruplex structure with hemin and shows strong peroxidase activity. When the complementary strand binds to the DNAzyme sequence, it blocks the formation of the G-quadraduplex structure and loses its peroxidase activity. In the presence of the target gene, PCR amplification proceeds, and anti-DNAzyme sequence modified primers present in the reaction mixture form a double strand through primer extension...
January 4, 2018: Analyst
https://www.readbyqxmd.com/read/29288978/expression-and-function-of-muc1-in-uterine-tissues-during-early-pregnancy-in-sheep-after-natural-oestrous-or-artificially-induced-oestrous
#3
Xiangguo Wang, Boyang Zhu, Shanhui Xiong, Xihui Sheng, Xiaolong Qi, Qizhen Huang, Chaolei Chen, Yong Guo, Hemin Ni
Mucin 1 (MUC1), a cell surface glycoprotein, is expressed mainly in the endometrial luminal epithelium (LE) and glandular epithelium (GE) of the endometrium in many mammalian species including mice, rats, pigs, sheep, horses and humans during various stages of a menstrual or oestrous cycle, where it plays an important role in embryo implantation and placentation. However, the expression and function of MUC1 in uterine tissues during early pregnancy in sheep after artificially-induced oestrous is not known. Therefore, we investigated the expression and function of MUC1 in the early pregnant and non-pregnant uterine tissues of sheep with natural oestrous or artificially-induced oestrous on days 10, 12, 14, 16 and 18 of the cycle by in situ hybridization, quantitative real-time polymerase chain reaction, immunohistochemical staining and western blotting methods...
December 20, 2017: Theriogenology
https://www.readbyqxmd.com/read/29212045/interactions-of-hemin-with-bovine-serum-albumin-and-human-hemoglobin-a-fluorescence-quenching-study
#4
Magdalena Makarska-Bialokoz
The binding interactions between hemin (Hmi) and bovine serum albumin (BSA) or human hemoglobin (HHb), respectively, have been examined in aqueous solution at pH=7.4, applying UV-vis absorption, as well as steady-state, synchronous and three-dimensional fluorescence spectra techniques. Representative results received for both BSA and HHb intrinsic fluorescence proceeding from the interactions with hemin suggest the formation of stacking non-covalent and non-fluorescent complexes in both the Hmi-BSA and Hmi-HHb systems, with highly possible concurrent formation of a coordinate bond between a group on the protein surface and the metal in Hmi molecule...
December 1, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29110341/hemin-and-bile-pigments-are-the-secondary-structure-regulators-of-intrinsically-disordered-antimicrobial-peptides
#5
Ferenc Zsila, Tünde Juhász, Szilvia Bősze, Kata Horváti, Tamás Beke-Somfai
The interaction of protoporphyrin compounds of human origin with the major bee venom component melittin (26 a.a., Z +6) and its hybrid derivative (CM15, 15 a.a., Z +6) were studied by a combination of various spectroscopic methods. Throughout a two-state, concentration-dependent process, hemin and its metabolites (biliverdin, bilirubin, bilirubin ditaurate) increase the parallel β-sheet content of the natively unfolded melittin, suggesting the oligomerization of the peptide chains. In contrast, α-helix promoting effect was observed with the also disordered but more cationic CM15...
November 7, 2017: Chirality
https://www.readbyqxmd.com/read/29089150/hemin-reduces-hmgb1-release-by-uvb-in-an-ampk-ho-1-dependent-pathway-in-human-keratinocytes-hacat-cells
#6
Eun Jung Park, Young Min Kim, Ki Churl Chang
BACKGROUND AND AIMS: High mobility group box 1 (HMGB1) plays an important role as a pro-inflammatory cytokine that regulates inflammation in various diseases. We hypothesized that hemin might reduce HMGB1 release through the induction of HO-1 in UVB-induced HaCaTs. METHODS: The effects of hemin on the release of HMGB1 in UVB exposure were evaluated. The mechanisms were investigated using various signal inhibitors and small interfering RNA techniques. RESULTS: Treatment with hemin inhibited reactive oxygen species (ROS) in UVB-induced HaCaTs in a dose-dependent manner...
October 28, 2017: Archives of Medical Research
https://www.readbyqxmd.com/read/29070524/engineering-oxidative-stability-in-human-hemoglobin-based-on-the-hb-providence-%C3%AE-k82d-mutation-and-genetic-crosslinking
#7
Michael Brad Strader, Rachel Bangle, Claire J Parker Siburt, Cornelius L Varnado, Jayashree Soman, Andres S Benitez Cardenas, Premila S Samuel, Eileen W Singleton, Alvin L Crumbliss, John S Olson, Abdu I Alayash
Previous work suggested that hemoglobin (Hb) tetramer formation slows autoxidation and hemin loss and that the naturally occurring mutant, Hb Providence (βK82D) is much more resistant to degradation by H2O2 We have examined systematically the effects of genetic crosslinking of Hb tetramers with and without the Hb Providence mutation on autoxidation, hemin loss, and reactions with H2O2, using native HbA and various wild-type recombinant Hbs as controls. Genetically crosslinked Hb Presbyterian (βN108K) was also examined as an example of a low oxygen affinity tetramer...
October 25, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29061046/visual-label-free-telomerase-activity-monitor-via-enzymatic-etching-of-gold-nanorods
#8
Haitang Yang, Anran Liu, Min Wei, Yuanjian Liu, Bingjing Lv, Wei Wei, Yuanjian Zhang, Songqin Liu
Early diagnosis and life-long surveillance are clinically important to improve the long-term survival of cancer patients. Telomerase activity is a valuable biomarker for cancer diagnosis, but its measurement often used complex label procedures. Herein, we designed a novel, simple, visual and label-free method for telomerase detection by using enzymatic etching of gold nanorods (GNRs). First, repeating (TTAGGG)x sequences were extented on telomerase substrate (TS) primer. It formed G-quadruplex under the help of Hemin and K(+)...
November 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28871452/click-chemistry-mediated-cyclic-cleavage-of-metal-ion-dependent-dnazymes-for-amplified-and-colorimetric-detection-of-human-serum-copper-ii
#9
Daxiu Li, Jiaqing Xie, Wenjiao Zhou, Bingying Jiang, Ruo Yuan, Yun Xiang
The determination of the level of Cu(2+) plays important roles in disease diagnosis and environmental monitoring. By coupling Cu(+)-catalyzed click chemistry and metal ion-dependent DNAzyme cyclic amplification, we have developed a convenient and sensitive colorimetric sensing method for the detection of Cu(2+) in human serums. The target Cu(2+) can be reduced by ascorbate to form Cu(+), which catalyzes the azide-alkyne cycloaddition between the azide- and alkyne-modified DNAs to form Mg(2+)-dependent DNAzymes...
September 4, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28870318/assay-of-dna-methyltransferase-1-activity-based-on-uracil-specific-excision-reagent-digestion-induced-g-quadruplex-formation
#10
Jinlong Li, Guangwu He, Chaoli Mu, Keming Wang, Yang Xiang
DNA methylation catalyzed by DNA methyltransferase plays an important role in many biological processes including gene transcription, genomic imprinting and cellular differentiation. Herein, a novel and effective electrochemical method for the assay of DNA methyltransferase 1(DNMT1) activity has been successfully developed by using uracil-specific excision reagent (USER) induced G-quadruplex formation. Briefly, double stranded DNA containing the recognition sequence of DNMT1 is immobilized on the electrode...
September 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28852807/construction-of-a-highly-sensitive-signal-on-aptasensor-based-on-gold-nanoparticles-functionalized-silica-nanoparticles-for-selective-detection-of-tryptophan
#11
Ayemeh Bagheri Hashkavayi, Jahan Bakhsh Raoof, Reza Ojani
In this work, a highly sensitive, low-cost, and label-free aptasensor based on signal-on mechanisms of response was developed by immobilizing the aptamer on gold nanoparticles (AuNPs)/amine-functionalized silica nanoparticle (FSN)/screen-printed electrode (SPE) surface for highly selective electrochemical detection of tryptophan (Trp). The hemin (Hem), which interacted with the guanine bases of the aptamer, worked as a redox indicator to generate a readable electrochemical signal. The changes in the charge transfer resistance have been monitored using the voltammetry and electrochemical impedance spectroscopic (EIS) techniques...
August 29, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28738658/an-ultrasensitive-chemiluminescence-aptasensor-for-thrombin-detection-based-on-iron-porphyrin-catalyzing-luminescence-desorbed-from-chitosan-modified-magnetic-oxide-graphene-composite
#12
Yuanling Sun, Yanhui Wang, Jianbo Li, Chaofan Ding, Yanna Lin, Weiyan Sun, Chuannan Luo
In this work, an ultrasensitive chemiluminescence (CL) aptasensor was prepared for thrombin detection based on iron porphyrin catalyzing luminol - hydrogen peroxide luminescence under alkaline conditions, and iron porphyrin was desorbed from chitosan modified magnetic oxide graphene composite (CS@Fe3O4@GO). Firstly, CS@Fe3O4@GO was prepared. CS@Fe3O4@GO has advantages of the good biocompatibility and positively charged on its surface of CS, the large specific surface area of GO and the easy separation characteristics of Fe3O4...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28716864/alpha1-antitrypsin-binds-hemin-and-prevents-oxidative-activation-of-human-neutrophils-putative-pathophysiological-significance
#13
Sabina Janciauskiene, Srinu Tumpara, Malgorzata Wiese, Sabine Wrenger, Vijith Vijayan, Faikah Gueler, Rongjun Chen, Kukuh Madyaningrana, Ravi Mahadeva, Tobias Welte, Stephan Immenschuh, Joanna Chorostowska-Wynimko
Heme is a ubiquitous compound of human tissues, and it is involved in cellular physiology and metabolism. Once released from the cell, free heme oxidizes to the ferric state (hemin). High levels of hemin can cause oxidative stress and inflammation if not neutralized immediately by specialized scavenger proteins. Human alpha1-antitrypsin (A1AT), an acute-phase glycoprotein and important inhibitor of neutrophil proteases, is also a hemin-binding protein. A short-term exposure of freshly isolated human blood neutrophils to 4 µM hemin results in cell spreading, surface expression of filament protein, vimentin, free radical production, expression of heme oxygenase-1 (HO-1), release of IL-8, and enhanced neutrophil adhesion to human endothelial cells...
October 2017: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/28707876/fluorescence-light-up-biosensor-for-microrna-based-on-the-distance-dependent-photoinduced-electron-transfer
#14
Shasha Lu, Shuang Wang, Jiahui Zhao, Jian Sun, Xiurong Yang
It is demonstrated that miRNAs exhibit significant regulatory roles in a series of biological processes and associated with diverse human diseases. Herein, we report a convenient fluorescent biosensor for the quantitative determination of miR-21, a key miRNA related to cardio-cerebrovascular diseases. Our proposal involves not only the rational design of single stranded DNA as the probe, successively including a C-rich sequence as the synthetic template of DNA/Ag nanoclusters (DNA/AgNCs), a complementary (Com) sequence to hybridize with the miR-21, and a G-rich sequence to form a complex of G-quadruplex/hemin but also the distance-dependent property of photoinduced electron transfer (PET) between the preformed DNA/AgNCs (electron donor) and G-quadruplex/hemin complex (electron acceptor)...
August 1, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28701754/clostridium-perfringens-%C3%AE-toxin-impairs-erythropoiesis-by-inhibition-of-erythroid-differentiation
#15
Teruhisa Takagishi, Masaya Takehara, Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masahiro Nagahama
Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119(+) erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28699284/a-monocyte-tnf-endothelial-activation-axis-in-sickle-transgenic-mice-therapeutic-benefit-from-tnf-blockade
#16
Anna Solovey, Arif Somani, John D Belcher, Liming Milbauer, Lucile Vincent, Rafal Pawlinski, Karl A Nath, Robert J Kelm, Nigel Mackman, M Gerard O'Sullivan, Kalpna Gupta, Gregory M Vercellotti, Robert P Hebbel
Elaboration of tumor necrosis factor (TNF) is a very early event in development of ischemia/reperfusion injury pathophysiology. Therefore, TNF may be a prominent mediator of endothelial cell and vascular wall dysfunction in sickle cell anemia, a hypothesis we addressed using NY1DD, S+S(Antilles) , and SS-BERK sickle transgenic mice. Transfusion experiments revealed participation of abnormally activated blood monocytes exerting an endothelial activating effect, dependent upon Egr-1 in both vessel wall and blood cells, and upon NFκB(p50) in a blood cell only...
November 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28642757/the-fungal-pathogen-candida-glabrata-does-not-depend-on-surface-ferric-reductases-for-iron-acquisition
#17
Franziska Gerwien, Abu Safyan, Stephanie Wisgott, Sascha Brunke, Lydia Kasper, Bernhard Hube
Iron acquisition is a crucial virulence determinant for many bacteria and fungi, including the opportunistic fungal pathogens Candida albicans and C. glabrata. While the diverse strategies used by C. albicans for obtaining iron from the host are well-described, much less is known about the acquisition of this micronutrient from host sources by C. glabrata - a distant relative of C. albicans with closer evolutionary ties to Saccharomyces cerevisiae, which nonetheless causes severe clinical symptoms in humans...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28583303/keap1-knockdown-in-melanocytes-induces-cell-proliferation-and-survival-via-ho-1-associated-%C3%AE-catenin-signaling
#18
Ji Young Kim, Hemin Lee, Eun Jung Lee, Mikyoung Kim, Tae-Gyun Kim, Hyoung-Pyo Kim, Sang Ho Oh
BACKGROUND: Nrf2-Keap1 signaling pathway protects cells against photo-oxidative stress. Yet in recent works, its role in melanogenesis together with cell protection functions against oxidative stress has been gaining interest. However, its effect on melanogenesis still has contradictory results from different studies. OBJECTIVE: The aims of our study were to investigate the effect of Keap1 silencing in melanocyte on melanogenesis and its associated mechanism. METHODS: Primary human epidermal melanocytes and melan-a cell line were used for this experiment...
October 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28561084/a-label-free-colorimetric-biosensor-for-sensitive-detection-of-vascular-endothelial-growth-factor-165
#19
Huige Zhang, Liang Peng, Maoxing Li, Ji Ma, Shengda Qi, Hongli Chen, Lei Zhou, Xingguo Chen
Sensitive detection of a low abundant protein is essential for biomedical research and clinical diagnostics. Herein, we develop a label-free colorimetric biosensor for the sensitive detection of recombinant human vascular endothelial growth factor-165 (VEGF165). This biosensor consists of an aptamer-based hairpin probe, an assistant DNA-trigger duplex and a linear template. In the presence of VEGF165, the specific binding of VEGF165 with the aptamer-based hairpin probe results in the opening of a hairpin probe and the opened hairpin probe subsequently hybridizes with the single-stranded region of the assistant DNA-trigger duplex to initiate the strand displacement amplification (SDA) to yield abundant triggers...
May 31, 2017: Analyst
https://www.readbyqxmd.com/read/28534562/k-junction-structure-mediated-exponential-signal-amplification-strategy-for-microrna-detection-in-electrochemiluminescence-biosensor
#20
Xue Li, Anyi Chen, Yaqin Chai, Ruo Yuan
Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at the 5'-terminus. Thus, the activity of the reporter DNA could be inhibited and the recognition domain for the target microRNA constructed through the K-junction structure formed...
May 23, 2017: Analyst
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