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Human hemin

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https://www.readbyqxmd.com/read/28642757/the-fungal-pathogen-candida-glabrata-does-not-depend-on-surface-ferric-reductases-for-iron-acquisition
#1
Franziska Gerwien, Abu Safyan, Stephanie Wisgott, Sascha Brunke, Lydia Kasper, Bernhard Hube
Iron acquisition is a crucial virulence determinant for many bacteria and fungi, including the opportunistic fungal pathogens Candida albicans and C. glabrata. While the diverse strategies used by C. albicans for obtaining iron from the host are well-described, much less is known about the acquisition of this micronutrient from host sources by C. glabrata - a distant relative of C. albicans with closer evolutionary ties to Saccharomyces cerevisiae, which nonetheless causes severe clinical symptoms in humans...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28583303/keap1-knockdown-in-melanocytes-induces-cell-proliferation-and-survival-via-ho-1-associated-%C3%AE-catenin-signaling
#2
Ji Young Kim, Hemin Lee, Eun Jung Lee, Mikyoung Kim, Tae-Gyun Kim, Hyoung-Pyo Kim, Sang Ho Oh
BACKGROUND: Nrf2-Keap1 signaling pathway protects cells against photo-oxidative stress. Yet in recent works, its role in melanogenesis together with cell protection functions against oxidative stress has been gaining interest. However, its effect on melanogenesis still has contradictory results from different studies. OBJECTIVE: The aims of our study were to investigate the effect of Keap1 silencing in melanocyte on melanogenesis and its associated mechanism. METHODS: Primary human epidermal melanocytes and melan-a cell line were used for this experiment...
May 18, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28561084/a-label-free-colorimetric-biosensor-for-sensitive-detection-of-vascular-endothelial-growth-factor-165
#3
Huige Zhang, Liang Peng, Maoxing Li, Ji Ma, Shengda Qi, Hongli Chen, Lei Zhou, Xingguo Chen
Sensitive detection of a low abundant protein is essential for biomedical research and clinical diagnostics. Herein, we develop a label-free colorimetric biosensor for the sensitive detection of recombinant human vascular endothelial growth factor-165 (VEGF165). This biosensor consists of an aptamer-based hairpin probe, an assistant DNA-trigger duplex and a linear template. In the presence of VEGF165, the specific binding of VEGF165 with the aptamer-based hairpin probe results in the opening of a hairpin probe and the opened hairpin probe subsequently hybridizes with the single-stranded region of the assistant DNA-trigger duplex to initiate the strand displacement amplification (SDA) to yield abundant triggers...
May 31, 2017: Analyst
https://www.readbyqxmd.com/read/28534562/k-junction-structure-mediated-exponential-signal-amplification-strategy-for-microrna-detection-in-electrochemiluminescence-biosensor
#4
Xue Li, Anyi Chen, Yaqin Chai, Ruo Yuan
Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as a catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at the 5'-terminus. Thus, the activity of the reporter DNA could be inhibited and the recognition domain for the target microRNA constructed through the K-junction structure formed...
May 23, 2017: Analyst
https://www.readbyqxmd.com/read/28525849/novel-photoelectrochemical-immunosensor-for-disease-related-protein-assisted-by-hemin-g-quadruplex-based-dnazyme-on-gold-nanoparticles-to-enhance-cathodic-photocurrent-on-p-cubi2o4-semiconductor
#5
Shuzhen Lv, Kangyao Zhang, Zhenzhen Lin, Dianping Tang
A novel p-type semiconductor material (p-CuBi2O4) is designed for the construction of split-type photoelectrochemical (PEC) immunosensor for alpha-fetoprotein (AFP) with the hemin assistant to enhance the cathodic photocurrent. Initially, the photocathode of PEC immunosensor is fabricated by p-CuBi2O4 on a layer of gold nanoparticles (AuNPs, as a front contact of p-CuBi2O4) to enhance the efficiency of charge separation. In the presence of target AFP, a sandwich-type immunoreaction was carried out in capture antibody-coated microplate by using detection antibody and hemin-based G-quadruplex (labeled on the AuNP) as the signal probe...
May 13, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28513652/an-electrochemiluminescence-assay-for-sensitive-detection-of-methyltransferase-activity-in-different-cancer-cells-by-hybridization-chain-reaction-coupled-with-a-g-quadruplex-hemin-dnazyme-biosensing-strategy
#6
Hui Zhang, Zhihui Guo, Huilei Dong, Hongfei Chen, Chenxin Cai
In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (ΔI) allowed detection of DNMT1 activity down to 0.09 U mL(-1), and ΔI was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL(-1) in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells...
May 17, 2017: Analyst
https://www.readbyqxmd.com/read/28512172/in-vivo-hemin-conditioning-targets-the-vascular-and-immunological-compartments-and-restrains-prostate-tumor-development
#7
Felipe M Jaworski, Lucas Gentilini, Geraldine Gueron, Roberto P Meiss, Emiliano G Ortiz, Paula M Berguer, Asif Ahmed, Nora M Navone, Gabriel A Rabinovich, Daniel Compagno, Diego J Laderach, Elba S Vazquez
Purpose: Conditioning strategies constitute a relatively unexplored and exciting opportunity to shape tumor fate by targeting the tumor microenvironment. In this study we assessed how hemin, a pharmacological inducer of Heme Oxygenase-1 (HO-1), impacts upon prostate cancer (PCa) development in an in vivo conditioning model. <p>Experimental Design: The stroma of C57BL/6 mice was conditioned by subcutaneous administration of hemin prior to TRAMP-C1 tumor challenge. Complementary in vitro and in vivo assays were performed to evaluate hemin effect on both angiogenesis and the immune response...
May 16, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28499167/electrochemical-behavior-of-hemin-binding-with-human-centrin-3
#8
Yaqin Zhao, Xuefeng Chu, Binsheng Yang
The electrochemical responses of human centrin 3 (HsCen3) binding with hemin were studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) using glassy carbon electrodes (GCEs). In CV, the formal potential (E(0')) of hemin with the addition of HsCen3 shifted from -0.51 to -0.36V (versus saturated calomel electrode, SCE), indicating that a new species of hemin-HsCen3 had formed. Upon binding with HsCen3, the redox current of hemin in CV and DPV decreased significantly. Based on their titration curves, the association constant of HsCen3 with hemin was obtained with a logK of approximately 4, which was consistent with that obtained from spectroscopy...
April 29, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28448124/bioinspired-engineering-of-poly-ethylene-glycol-hydrogels-and-natural-protein-fibers-for-layered-heart-valve-constructs
#9
Qian Li, Yun Bai, Tao Jin, Shuo Wang, Wei Cui, Ilinca Stanciulescu, Rui Yang, Hemin Nie, Linshan Wang, Xing Zhang
Layered constructs from poly(ethylene glycol) (PEG) hydrogels and chicken eggshell membranes (ESMs) are fabricated, which can be further cross-linked by glutaraldehyde (GA) to form GA-PEG-ESM composites. Our results indicate that ESMs composed of protein fibrous networks show elastic moduli ∼3.3-5.0 MPa and elongation percentages ∼47-56%, close to human heart valve leaflets. Finite element simulations reveal obvious stress concentration on a partial number of fibers in the GA-cross-linked ESM (GA-ESM) samples, which can be alleviated by efficient stress distribution among multiple layers of ESMs embedded in PEG hydrogels...
May 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28412658/cds-mn-quantum-dot-functionalized-g-c3n4-nanohybrids-as-signal-generation-tags-for-photoelectrochemical-immunoassay-of-prostate-specific-antigen-coupling-dnazyme-concatamer-with-enzymatic-biocatalytic-precipitation
#10
Kangyao Zhang, Shuzhen Lv, Zhenzhen Lin, Dianping Tang
A new photoelectrochemical (PEC) immunosensor based on Mn-doped CdS quantum dots (CdS:Mn QDs) on g-C3N4 nanosheets was developed for the sensitive detection of prostate specific antibody (PSA) in biological fluids. The signal derived from the as-synthesized Cd:Mn QDs-functionalized g-C3N4 nanohybrids via a hydrothermal method and was amplified through DNAzyme concatamers on gold nanoparticles accompanying enzymatic biocatalytic precipitation. Experimental results by UV-vis absorption spectra and photoluminescence revealed that CdS:Mn QDs/g-C3N4 nanohybrids exhibited higher photocurrent than those of CdS:Mn QDs and g-C3N4 alone...
September 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28366217/biosensing-platform-for-the-detection-of-uric-acid-based-on-graphene-quantum-dots-and-g-quadruplex-hemin-dnazyme
#11
Nan Cai, Lu Tan, Yan Li, Tingting Xia, Tianyu Hu, Xingguang Su
In this paper, a label-free biosensing platform for fluorescence detection of uric acid was designed on the peroxidase-mimicking activities of G-quadruplex/hemin DNAzyme and the introduction of caffeic acid. Uric acid could be decomposed by uricase and then produced hydrogen peroxide and allantoin. We thus successfully achieved the indirect detection of uric acid by monitoring the concentration of hydrogen peroxide. The G-quadruplex/hemin DNAzyme could act as peroxidase and decompose the hydrogen peroxide into hydroxyl radicals at room temperature...
May 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28316021/hemin-protects-against-oxygen-glucose-deprivation-induced-apoptosis-activation-via-neuroglobin-in-sh-sy5y-cells
#12
Yun-Jia Wang, Qian-Yi Peng, Song-Yun Deng, Cai-Xia Chen, Long Wu, Li Huang, Li-Na Zhang
This study aimed to investigate the mechanism underlying the neuroprotective effect of hemin in oxygen-glucose deprivation (OGD)-treated neurons. OGD-treated SH-SY5Y cells (human neuroblastoma cells) were used in the study. The cellular viability of SH-SY5Y cells was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the cell apoptosis rate was determined by flow cytometry analysis with Annexin V-fluorescein isothiocyanate and propidium iodide staining with or without hemin pretreatment...
March 18, 2017: Neurochemical Research
https://www.readbyqxmd.com/read/28281267/a-novel-colorimetric-pcr-based-biosensor-for-detection-and-quantification-of-hepatitis-b-virus
#13
Li Yang, Mei Li, Feng Du, Gangyi Chen, Afshan Yasmeen, Zhuo Tang
Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that puts people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has infected one-third of the worldwide population, and 350 million people suffer from chronic HBV infection. For these reasons, development of an accurate, sensitive, and expedient detection method for diagnosing, monitoring, and assessing therapeutic response of HBV is very necessary and urgent for public health and disease control...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28270832/actin-family-proteins-in-the-human-ino80-chromatin-remodeling-complex-exhibit-functional-roles-in-the-induction-of-heme-oxygenase-1-with-hemin
#14
Yuichiro Takahashi, Hirokazu Murakami, Yusuke Akiyama, Yasutake Katoh, Yukako Oma, Hitoshi Nishijima, Kei-Ichi Shibahara, Kazuhiko Igarashi, Masahiko Harata
Nuclear actin family proteins, comprising of actin and actin-related proteins (Arps), are essential functional components of the multiple chromatin remodeling complexes. The INO80 chromatin remodeling complex, which is evolutionarily conserved and has roles in transcription, DNA replication and repair, consists of actin and actin-related proteins Arp4, Arp5, and Arp8. We generated Arp5 knockout (KO) and Arp8 KO cells from the human Nalm-6 pre-B cell line and used these KO cells to examine the roles of Arp5 and Arp8 in the transcriptional regulation mediated by the INO80 complex...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28243792/hmox1-as-a-marker-of-iron-excess-induced-adipose-tissue-dysfunction-affecting-glucose-uptake-and-respiratory-capacity-in-human-adipocytes
#15
José María Moreno-Navarrete, Francisco Ortega, Amaia Rodríguez, Jèssica Latorre, Sara Becerril, Mònica Sabater-Masdeu, Wifredo Ricart, Gema Frühbeck, José Manuel Fernández-Real
AIMS/HYPOTHESIS: Iron excess in adipose tissue is known to promote adipose tissue dysfunction. Here, we aimed to investigate the possible role of haem oxygenase 1 (HMOX1) in iron excess-induced adipose tissue dysfunction. METHODS: Cross-sectionally, HMOX1 gene expression in subcutaneous and visceral adipose tissue was analysed in two independent cohorts (n = 234 and 40) in relation to obesity. We also evaluated the impact of weight loss (n = 21), weight gain (in rats, n = 20) on HMOX1 mRNA; HMOX1 mRNA levels during human adipocyte differentiation; the effects of inflammation and iron on adipocyte HMOX1; and the effects of HMOX1-induced activity on adipocyte mitochondrial respiratory function, glucose uptake and adipogenesis...
February 27, 2017: Diabetologia
https://www.readbyqxmd.com/read/28171788/fabrication-of-a-novel-hemin-based-monolithic-column-and-its-application-in-separation-of-protein-from-complex-bio-matrix
#16
Xiaoya Jiang, Doudou Zhang, Xueying Li, Xixi Wang, Ligai Bai, Haiyan Liu, Hongyuan Yan
A novel polymer-based monolithic column was prepared via redox initiation system within the confines of a stainless steel column with 4.6mm i.d. In the processes, hemin and lauryl methacrylate were used as co-monomers; ethylene dimethacrylate as crosslinking agent; n-butyl alcohol, ethanediol, and N, N-dimethylformamide as tri-porogens; benzoyl peroxide and N, N-dimethyl aniline as redox initiation system. The resulting polymer-based monolithic columns were characterized by scanning electron microscopy, nitrogen adsorption-desorption instrument, and mercury intrusion porosimeter, respectively...
January 19, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28163966/an-active-principle-of-nigella-sativa-l-thymoquinone-showing-significant-antimicrobial-activity-against-anaerobic-bacteria
#17
Mohammad Akram Randhawa, Awwad Khalaf Alenazy, Majed Gorayan Alrowaili, Jamith Basha
AIM/BACKGROUND: Thymoquinone (TQ) is the major active principle of Nigella sativa seed (black seed) and is known to control many fungi, bacteria, and some viruses. However, the activity of TQ against anaerobic bacteria is not well demonstrated. Anaerobic bacteria can cause severe infections, including diarrhea, aspiration pneumonia, and brain abscess, particularly in immunodeficient individuals. The present study aimed to investigate the in vitro antimicrobial activity of TQ against some anaerobic pathogens in comparison to metronidazole...
January 2017: Journal of Intercultural Ethnopharmacology
https://www.readbyqxmd.com/read/28109528/specific-enrichment-of-a-targeted-nitrotyrosine-containing-peptide-from-complex-matrices-and-relative-quantification-for-liquid-chromatography-mass-spectrometry-analysis
#18
Yun Yang
Protein tyrosine nitration is considered an important non-enzymatic post-translational modification. In the tyrosine nitration process, 3-nitrotyrosine is formed and recognized as a biomarker of nitrosative/nitrative stress implicated in inflammatory responses and age-related disorders. In view of the complexity of biological samples and the ultra-low abundance of protein-incorporated nitrotyrosine, selective enrichment of nitrotyrosine-containing peptides prior to chromatographic separation is crucial. Herein, I report a simple yet highly specific and efficient enrichment method for nitrotyrosine-containing peptides...
February 17, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28105837/hrp-mimicking-dnazyme-catalyzed-in-situ-generation-of-polyaniline-to-assist-signal-amplification-for-ultrasensitive-surface-plasmon-resonance-biosensing
#19
Haiyin Li, Jiafu Chang, Ting Hou, Feng Li
It is well-known that the horseradish peroxidase- (HRP-) mimicking DNAzyme, namely, hemin/G-quadruplex, can effectively catalyze the polymerization of aniline to form DNA-guided polyaniline. Meanwhile, polyaniline exhibits extraordinary electrical, electrochemical, and redox properties, as well as excellent SPR signal-enhancing ability. Herein, we report a novel ultrasensitive surface plasmon resonance (SPR) biosensor based on HRP-mimicking DNAzyme-catalyzed in situ formation of polyaniline for signal amplification, using bleomycin (BLM) as the proof-of-concept analyte...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28068513/nrf2-dependent-induction-of-innate-host-defense-via-heme-oxygenase-1-inhibits-zika-virus-replication
#20
Hanxia Huang, Barry Falgout, Kazuyo Takeda, Kenneth M Yamada, Subhash Dhawan
We identified primary human monocyte-derived macrophages (MDM) as vulnerable target cells for Zika virus (ZIKV) infection. We demonstrate dramatic effects of hemin, the natural inducer of the heme catabolic enzyme heme oxygenase-1 (HO-1), in the reduction of ZIKV replication in vitro. Both LLC-MK2 monkey kidney cells and primary MDM exhibited hemin-induced HO-1 expression with major reductions of >90% in ZIKV replication, with little toxicity to infected cells. Silencing expression of HO-1 or its upstream regulatory gene, nuclear factor erythroid-related factor 2 (Nrf2), attenuated hemin-induced suppression of ZIKV infection, suggesting an important role for induction of these intracellular mediators in retarding ZIKV replication...
March 2017: Virology
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