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Human hemin

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https://www.readbyqxmd.com/read/28068513/nrf2-dependent-induction-of-innate-host-defense-via-heme-oxygenase-1-inhibits-zika-virus-replication
#1
Hanxia Huang, Barry Falgout, Kazuyo Takeda, Kenneth M Yamada, Subhash Dhawan
We identified primary human monocyte-derived macrophages (MDM) as vulnerable target cells for Zika virus (ZIKV) infection. We demonstrate dramatic effects of hemin, the natural inducer of the heme catabolic enzyme heme oxygenase-1 (HO-1), in the reduction of ZIKV replication in vitro. Both LLC-MK2 monkey kidney cells and primary MDM exhibited hemin-induced HO-1 expression with major reductions of >90% in ZIKV replication, with little toxicity to infected cells. Silencing expression of HO-1 or its upstream regulatory gene, nuclear factor erythroid-related factor 2 (Nrf2), attenuated hemin-induced suppression of ZIKV infection, suggesting an important role for induction of these intracellular mediators in retarding ZIKV replication...
January 6, 2017: Virology
https://www.readbyqxmd.com/read/28013165/in-vitro-cytotoxicity-evaluation-of-graphene-oxide-from-the-peroxidase-like-activity-perspective
#2
Wei Zhang, Ying Sun, Zhichao Lou, Lina Song, Yang Wu, Ning Gu, Yu Zhang
In this study, PEGylated graphene oxide (PEG-GO)-hemin composite structure was constructed. Hemin in the form of nanoscaled aggregates were immobilized on PEG-GO sheets by the π-π stacking super-molecular interaction. Via catalyzing the oxidation of chromogenic substrates, we elicited the obtained PEG-GO-Hemin composite sheets have much higher peroxidase-like activity compared to hemin or PEG-GO alone, which is due to the introduction of enzyme active center of hemin with high dispersity, the excellent affinity to organic substrate through π-π stacking and/or electrostatic adsorption and the rapid electron transfer capability of PEG-GO...
December 19, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28011635/hemoglobin-kirklareli-%C3%AE-h58l-a-new-variant-associated-with-fe-deficiency-and-increased-co-binding
#3
Emmanuel Bissé, Christine Schaeffer-Reiss, Alain Van Dorsselaer, Tchilabalo Dilezitoko Alayi, Thomas Epting, Karl Winkler, Andres S Benitez Cardenas, Jayashree Soman, Ivan Birukou, Premila P Samuel, John S Olson
Mutations in hemoglobin can cause a wide range of phenotypic outcomes, including anemia due to protein instability and red cell lysis. Uncovering the biochemical basis for these phenotypes can provide new insights into hemoglobin structure and function as well as identify new therapeutic opportunities. We report here a new hemoglobin α chain variant in a female patient with mild anemia, whose father also carries the trait and is from the Turkish city of Kirklareli. Both the patient and her father had a His58(E7)→Leu mutation in α1...
December 23, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27995330/piceatannol-attenuates-homocysteine-induced-endoplasmic-reticulum-stress-and-endothelial-cell-damage-via-heme-oxygenase-1-expression
#4
Jin-Sang Kil, Sun-Oh Jeong, Hun-Taeg Chung, Hyun-Ock Pae
A growing body of evidence implicates endoplasmic reticulum (ER)-induced cellular dysfunction and apoptosis as important factors to a variety of diseases. In endothelial cells (ECs), the sulfur-containing amino acid homocysteine (Hcy) causes EC apoptosis and reactive oxygen species (ROS) generation through induction of ER stress. Here, we have investigated whether piceatannol (Pic), a resveratrol analog, could protect ECs against Hcy-induced apoptosis, oxidative stress and ER stress, with specific emphasis on heme oxygenase-1 (HO-1)...
December 20, 2016: Amino Acids
https://www.readbyqxmd.com/read/27974865/association-of-heme-oxygenase-1-with-lung-protection-in-malaria-associated-ali-ards
#5
Marcelo L M Pereira, Luana S Ortolan, Michelle K Sercundes, Daniela Debone, Oscar Murillo, Flávia A Lima, Claudio R F Marinho, Sabrina Epiphanio
Malaria is a serious disease, caused by the parasite of the genus Plasmodium, which was responsible for 440,000 deaths in 2015. Acute lung injury/acute respiratory distress syndrome (ALI/ARDS) is one of the main clinical complications in severe malaria. The murine model DBA/2 reproduces the clinical signs of ALI/ARDS in humans, when infected with Plasmodium berghei ANKA. High levels of HO-1 were reported in cases of severe malaria. Our data indicated that the HO-1 mRNA and protein expression are increased in mice that develop malaria-associated ALI/ARDS (MA-ALI/ARDS)...
2016: Mediators of Inflammation
https://www.readbyqxmd.com/read/27940243/sigch-an-extracytoplasmic-function-sigma-factor-of-porphyromonas-gingivalis-regulates-the-expression-of-cdhr-and-hmuyr
#6
Koki Ota, Yuichiro Kikuchi, Kentaro Imamura, Daichi Kita, Kouki Yoshikawa, Atsushi Saito, Kazuyuki Ishihara
Extracytoplasmic function (ECF) sigma factors play an important role in the bacterial response to various environmental stresses. Porphyromonas gingivalis, a prominent etiological agent in human periodontitis, possesses six putative ECF sigma factors. So far, information is limited on the ECF sigma factor, PGN_0319. The aim of this study was to investigate the role of PGN_0319 (SigCH) of P. gingivalis, focusing on the regulation of hmuY and hmuR, which encode outer-membrane proteins involved in hemin utilization, and cdhR, a transcriptional regulator of hmuYR...
December 8, 2016: Anaerobe
https://www.readbyqxmd.com/read/27936861/effects-of-hemin-co2-and-ph-on-the-branching-of-candida-albicans-filamentous-forms
#7
Ágnes Jakab, Károly Antal, Tamás Emri, Imre Boczonádi, Alexandra Imre, Enikő Gebri, László Majoros, Walter Péter Pfliegler, Máté Szarka, György Balla, József Balla, István Pócsi
Morphological transitions of wild-type and oxidative stress-tolerant Candida albicans strains were followed in the RPMI-FBS culture medium at pH values and CO2 levels characteristic for the anatomical niches inhabited by this opportunistic human pathogen fungus, including the oral cavity as well as the intestinal and vaginal lumens. Selected cultures were also supplemented with hemin modeling bleedings. Germination as well as elongation and branching of hyphae were monitored in the cultures using time-lapse video microscopy...
December 2016: Acta Microbiologica et Immunologica Hungarica
https://www.readbyqxmd.com/read/27920765/novel-pathways-for-ameliorating-the-fitness-cost-of-gentamicin-resistant-small-colony-variants
#8
Martin Vestergaard, Wilhelm Paulander, Bingfeng Leng, Jesper B Nielsen, Henrik T Westh, Hanne Ingmer
Small colony variants (SCVs) of the human pathogen Staphylococcus aureus are associated with persistent infections. Phenotypically, SCVs are characterized by slow growth and they can arise upon interruption of the electron transport chain that consequently reduce membrane potential and thereby limit uptake of aminoglycosides (e.g., gentamicin). In this study, we have examined the pathways by which the fitness cost of SCVs can be ameliorated. Five gentamicin resistant SCVs derived from S. aureus JE2 were independently selected on agar plates supplemented with gentamicin...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27902920/an-ultrasensitive-aptasensor-for-hemin-and-hemoglobin-based-on-signal-amplification-via-electrocatalytic-oxygen-reduction
#9
Zahra Shekari, Hamid R Zare, Ali Falahati
The present study aims at the fabrication of a novel electrochemical aptasensor, Ap-GA-AMSN-GCE, for the label-free determination of hemin and hemoglobin (Hb). Basically, the electrochemical reduction current of hemin or Hb incubated on Ap-GA-AMSN-GCE in the presence of oxygen serves as an excellent signal for quantitative determination of these analytes. By differential pulse voltammetry, the calibration plot was linear in the concentration range of 1.0 × 10(-19)-1.0 × 10(-6) M of hemin and Hb. Also, the detection limits, DL, of hemin and Hb were found to be 7...
February 1, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/27884416/hemin-ameliorates-influenza-pneumonia-by-attenuating-lung-injury-and-regulating-the-immune-response
#10
Conghui Wang, Yanjing Zhang, LianLian Han, Li Guo, Hui Zhong, Jianwei Wang
The anti-influenza activity of hemin, an inducer, activator and the substrate of heme oxygenase-1 (HO-1), was examined both in vitro and in vivo. The human lung carcinoma cell line A549 was used to evaluate the in vitro effect of hemin on influenza A virus (IAV) replication. A mouse model was used to examine the in vivo activity of hemin. Observation indexes included survival rate and body weight of mice, virus load and pathological examination of the lungs, and characterization of the systemic and local immune responses...
January 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/27875751/all-in-one-dual-aptasensor-capable-of-rapidly-quantifying-carcinoembryonic-antigen
#11
Harriet Khang, Kelly Cho, Stephanie Chong, Ji Hoon Lee
Using a dual DNA aptamer (CEA aptamer linked to hemin aptamer), capable of rapidly capturing carcinoembryonic antigen (CEA) and hemin, an all-in-one dual-aptasensor with 1,1'-oxalyldiimidazole (ODI) chemiluminescence detection was developed for the early diagnosis of human cancer. CEA and hemin competitively bound with the dual DNA aptamer while the mixture in a detection cell was incubated for 30min at room temperature. When Amplex Red and H2O2 were added in the detection cell after the incubation, the yield of resorufin formed from the reaction Amplex Red and H2O2 depended on the concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemin and the dual DNA aptamer...
April 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27823668/a-label-free-ultrasensitive-assay-of-8-hydroxy-2-deoxyguanosine-in-human-serum-and-urine-samples-via-polyaniline-deposition-and-tetrahedral-dna-nanostructure
#12
Jiahui Fan, Yuanjian Liu, Ensheng Xu, Yuanjian Zhang, Wei Wei, Lihong Yin, Yuepu Pu, Songqin Liu
Oxidative damage is an important factor in causing various human disease and injury. As an oxidative DNA damage product, 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a key marker, which is widely used to study oxidative damage mechanism in diseases. Most reported electrochemical methods were based on oxidation current of 8-OHdG. In this work, a simple electrochemical biosensor for ultrasensitive detection of 8-OHdG was proposed based on it triggered polyaniline (PANI) deposition on tetrahedral DNA nanostructure (TDN)...
November 23, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27815112/induction-of-heme-oxygenase-1-with-hemin-alleviates-cisplatin-induced-reproductive-toxicity-in-male-rats-and-enhances-its-cytotoxicity-in-prostate-cancer-cell-line
#13
Gehan Hussein Heeba, Alaaeldin Ahmed Hamza, Soha Osama Hassanin
Cisplatin-induced testicular damage is a major obstacle in the application of cisplatin as chemotherapeutic agent. However, it remains as one of the most widely employed anticancer agents in treating various solid tumors including prostate cancer. Since heme-oxygenase-1 (HO-1) is a cytoprotective enzyme with anti-oxidative stress, anti-inflammatory and anticancer activities, we investigated the effects of up-regulation of HO-1 by hemin and its inhibition by zinc protoporphyrin-IX (ZnPP) on cisplatin-induced testicular toxicity in adult rats...
December 15, 2016: Toxicology Letters
https://www.readbyqxmd.com/read/27811998/smart-nanocomposites-of-cu-hemin-metal-organic-frameworks-for-electrochemical-glucose-biosensing
#14
Juan He, Han Yang, Yayun Zhang, Jie Yu, Longfei Miao, Yonghai Song, Li Wang
Herein, a smart porous material, Cu-hemin metal-organic-frameworks (Cu-hemin MOFs), was synthesized via assembling of Cu(2+) with hemin to load glucose oxidase (GOD) for electrochemical glucose biosensing for the first time. The formation of the Cu-hemin MOFs was verified by scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, N2 adsorption/desorption isotherms, UV-vis absorption spectroscopy, fluorescence spectroscopy, thermal analysis and electrochemical techniques...
November 4, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27641735/microrna-1304-suppresses-human-non-small-cell-lung-cancer-cell-growth-in-vitro-by-targeting-heme-oxygenase-1
#15
Cheng-Gang Li, Meng-Fan Pu, Chun-Zhu Li, Man Gao, Ming-Xia Liu, Cun-Zhi Yu, Hong Yan, Chun Peng, Yang Zhao, Yu Li, Ze-Long Ma, Xin-Ming Qi, Yi-Zheng Wang, Ling-Ling Miao, Jin Ren
Previous studies have shown that microRNA-1304 (miR-1304) is dysregulated in certain types of cancers, including non-small cell lung cancer (NSCLC), and might be involved in tumor survival and/or growth. In this study we investigated the direct target of miR-1304 and its function in NSCLC in vitro. Human lung adenocarcinoma cell lines (A549 and NCI-H1975) were studied. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays. Cell apoptosis and cell cycle were examined using annexin V-PE/7-AAD and PI staining assays, respectively...
January 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/27624062/volumetric-analysis-of-formation-of-the-complex-of-g-quadruplex-dna-with-hemin-using-high-pressure
#16
Shuntaro Takahashi, Sudipta Bhowmik, Naoki Sugimoto
DNA guanine-quadruplexes (G-quadruplexes) complexed with the Fe-containing porphyrin, hemin (iron(III)-protoporphyrin IX), can catalyze oxidation reactions. This so-called DNAzyme has been widely used in the field of DNA nanotechnology. To improve DNAzyme properties, we sought to elucidate the interaction mechanism between G-quadruplex DNA and hemin. Here, we performed volumetric analyses of formation of the complex between an oligonucleotide with the sequence of human telomeric DNA (h-telo) and hemin. The G-quadruplex DNA alone and the G-quadruplex DNA-hemin complex were destabilized with increasing pressure in Na(+) buffer...
January 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/27619525/a-simple-fast-label-free-colorimetric-method-for-detection-of-telomerase-activity-in-urine-by-using-hemin-graphene-conjugates
#17
Xiaolin Xu, Min Wei, Yuanjian Liu, Xu Liu, Wei Wei, Yuanjian Zhang, Songqin Liu
Telomerase, a widely accepted cancer biomarker for early cancer diagnostics, is considered as an important therapeutic target. To now, it is still a challenging subject to develop a simple and sensitive strategy for telomerase activity detection. Herein, we reported a simple colorimetric strategy for label-free quantification of human telomerase activity in urine by using hemin-graphene nanomaterial (H-GNs). H-GNs possessed tailored dispersibility in the high salt concentration and highly active biomimetic oxidation catalyst property...
January 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27612006/peroxisome-proliferator-activated-receptor-%C3%AE-regulates-the-v-ets-avian-erythroblastosis-virus-e26-oncogene-homolog-1-microrna-27a-axis-to-reduce-endothelin-1-and-endothelial-dysfunction-in-the-sickle-cell-mouse-lung
#18
Bum-Yong Kang, Kathy Park, Jennifer M Kleinhenz, Tamara C Murphy, Roy L Sutliff, David Archer, C Michael Hart
Pulmonary hypertension (PH), a serious complication of sickle cell disease (SCD), causes significant morbidity and mortality. Although a recent study determined that hemin release during hemolysis triggers endothelial dysfunction in SCD, the pathogenesis of SCD-PH remains incompletely defined. This study examines peroxisome proliferator-activated receptor γ (PPARγ) regulation in SCD-PH and endothelial dysfunction. PH and right ventricular hypertrophy were studied in Townes humanized sickle cell (SS) and littermate control (AA) mice...
January 2017: American Journal of Respiratory Cell and Molecular Biology
https://www.readbyqxmd.com/read/27592241/target-triggered-catalytic-hairpin-assembly-and-tdt-catalyzed-dna-polymerization-for-amplified-electronic-detection-of-thrombin-in-human-serums
#19
Kai Shi, Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
Specific and sensitive detection of protein biomarkers is of great importance in biomedical and bioanalytical applications. In this work, a dual amplified signal enhancement approach based on the integration of catalytic hairpin assembly (CHA) and terminal deoxynucleotidyl transferase (TdT)-mediated in situ DNA polymerization has been developed for highly sensitive and label-free electrochemical detection of thrombin in human serums. The presence of the target thrombin leads to the unfolding and capture of a significant number of hairpin signal probes with free 3'-OH termini on the sensor electrode...
January 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27504793/cdte-cdse-quantum-dot-based-fluorescent-aptasensor-with-hemin-g-quadruplex-dnzyme-for-sensitive-detection-of-lysozyme-using-rolling-circle-amplification-and-strand-hybridization
#20
Zhenli Qiu, Jian Shu, Yu He, Zhenzhen Lin, Kangyao Zhang, Shuzhen Lv, Dianping Tang
Lysozyme with a small monomeric globular enzymatic protein is part of the innate immune system, and its deficiency can cause the increased incidence of disease. Herein, we devise a new signal-enhanced fluorescence aptasensing platform for quantitative screening of lysozyme by coupling with rolling circle amplification (RCA) and strand hybridization reaction, accompanying the assembly of CdTe/CdSe quantum dots (QDs) and hemin/G-quadruplex DNzyme. Initially, target-triggered release of the primer was carried out from DNA duplex via the reaction of the aptamer with the analyte, and the released primer could be then utilized as the template to produce numerous repeated oligonucleotide sequences by the RCA reaction...
January 15, 2017: Biosensors & Bioelectronics
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