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Human hemin

Martin Vestergaard, Wilhelm Paulander, Bingfeng Leng, Jesper B Nielsen, Henrik T Westh, Hanne Ingmer
Small colony variants (SCVs) of the human pathogen Staphylococcus aureus are associated with persistent infections. Phenotypically, SCVs are characterized by slow growth and they can arise upon interruption of the electron transport chain that consequently reduce membrane potential and thereby limit uptake of aminoglycosides (e.g., gentamicin). In this study, we have examined the pathways by which the fitness cost of SCVs can be ameliorated. Five gentamicin resistant SCVs derived from S. aureus JE2 were independently selected on agar plates supplemented with gentamicin...
2016: Frontiers in Microbiology
Zahra Shekari, Hamid R Zare, Ali Falahati
The present study aims at the fabrication of a novel electrochemical aptasensor, Ap-GA-AMSN-GCE, for the label-free determination of hemin and hemoglobin (Hb). Basically, the electrochemical reduction current of hemin or Hb incubated on Ap-GA-AMSN-GCE in the presence of oxygen serves as an excellent signal for quantitative determination of these analytes. By differential pulse voltammetry, the calibration plot was linear in the concentration range of 1.0 × 10(-19)-1.0 × 10(-6) M of hemin and Hb. Also, the detection limits, DL, of hemin and Hb were found to be 7...
November 27, 2016: Analytical Biochemistry
Conghui Wang, Yanjing Zhang, LianLian Han, Li Guo, Hui Zhong, Jianwei Wang
The anti-influenza activity of hemin, an inducer, activator and the substrate of heme oxygenase-1 (HO-1), was examined both in vitro and in vivo. The human lung carcinoma cell line A549 was used to evaluate the in vitro effect of hemin on influenza A virus (IAV) replication. A mouse model was used to examine the in vivo activity of hemin. Observation indexes included survival rate and body weight of mice, virus load and pathological examination of the lungs, and characterization of the systemic and local immune responses...
November 16, 2016: International Journal of Antimicrobial Agents
Harriet Khang, Kelly Cho, Stephanie Chong, Ji Hoon Lee
Using a dual DNA aptamer (CEA aptamer linked to hemin aptamer), capable of rapidly capturing carcinoembryonic antigen (CEA) and hemin, an all-in-one dual-aptasensor with 1,1'-oxalyldiimidazole (ODI) chemiluminescence detection was developed for the early diagnosis of human cancer. CEA and hemin competitively bound with the dual DNA aptamer while the mixture in a detection cell was incubated for 30min at room temperature. When Amplex Red and H2O2 were added in the detection cell after the incubation, the yield of resorufin formed from the reaction Amplex Red and H2O2 depended on the concentration of HRP-mimicking G-quardruplex DNAzyme formed from the binding interaction between hemin and the dual DNA aptamer...
November 16, 2016: Biosensors & Bioelectronics
Jiahui Fan, Yuanjian Liu, Ensheng Xu, Yuanjian Zhang, Wei Wei, Lihong Yin, Yuepu Pu, Songqin Liu
Oxidative damage is an important factor in causing various human disease and injury. As an oxidative DNA damage product, 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a key marker, which is widely used to study oxidative damage mechanism in diseases. Most reported electrochemical methods were based on oxidation current of 8-OHdG. In this work, a simple electrochemical biosensor for ultrasensitive detection of 8-OHdG was proposed based on it triggered polyaniline (PANI) deposition on tetrahedral DNA nanostructure (TDN)...
November 23, 2016: Analytica Chimica Acta
Gehan Hussein Heeba, Alaaeldin Ahmed Hamza, Soha Osama Hassanin
Cisplatin-induced testicular damage is a major obstacle in the application of cisplatin as chemotherapeutic agent. However, it remains as one of the most widely employed anticancer agents in treating various solid tumors including prostate cancer. Since heme-oxygenase-1 (HO-1) is a cytoprotective enzyme with anti-oxidative stress, anti-inflammatory and anticancer activities, we investigated the effects of up-regulation of HO-1 by hemin and its inhibition by zinc protoporphyrin-IX (ZnPP) on cisplatin-induced testicular toxicity in adult rats...
November 1, 2016: Toxicology Letters
Juan He, Han Yang, Yayun Zhang, Jie Yu, Longfei Miao, Yonghai Song, Li Wang
Herein, a smart porous material, Cu-hemin metal-organic-frameworks (Cu-hemin MOFs), was synthesized via assembling of Cu(2+) with hemin to load glucose oxidase (GOD) for electrochemical glucose biosensing for the first time. The formation of the Cu-hemin MOFs was verified by scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, N2 adsorption/desorption isotherms, UV-vis absorption spectroscopy, fluorescence spectroscopy, thermal analysis and electrochemical techniques...
November 4, 2016: Scientific Reports
Cheng-Gang Li, Meng-Fan Pu, Chun-Zhu Li, Man Gao, Ming-Xia Liu, Cun-Zhi Yu, Hong Yan, Chun Peng, Yang Zhao, Yu Li, Ze-Long Ma, Xin-Ming Qi, Yi-Zheng Wang, Ling-Ling Miao, Jin Ren
AIM: Previous studies have shown that microRNA-1304 (miR-1304) is dysregulated in certain types of cancers, including non-small cell lung cancer (NSCLC), and might be involved in tumor survival and/or growth. In this study we investigated the direct target of miR-1304 and its function in NSCLC in vitro. METHODS: Human lung adenocarcinoma cell lines (A549 and NCI-H1975) were studied. The cell proliferation and survival were investigated via cell counting, MTT and colony-formation assays...
September 19, 2016: Acta Pharmacologica Sinica
Shuntaro Takahashi, Sudipta Bhowmik, Naoki Sugimoto
DNA guanine-quadruplexes (G-quadruplexes) complexed with the Fe-containing porphyrin, hemin (iron(III)-protoporphyrin IX), can catalyze oxidation reactions. This so-called DNAzyme has been widely used in the field of DNA nanotechnology. To improve DNAzyme properties, we sought to elucidate the interaction mechanism between G-quadruplex DNA and hemin. Here, we performed volumetric analyses of formation of the complex between an oligonucleotide with the sequence of human telomeric DNA (h-telo) and hemin. The G-quadruplex DNA alone and the G-quadruplex DNA-hemin complex were destabilized with increasing pressure in Na(+) buffer...
September 2, 2016: Journal of Inorganic Biochemistry
Xiaolin Xu, Min Wei, Yuanjian Liu, Xu Liu, Wei Wei, Yuanjian Zhang, Songqin Liu
Telomerase, a widely accepted cancer biomarker for early cancer diagnostics, is considered as an important therapeutic target. To now, it is still a challenging subject to develop a simple and sensitive strategy for telomerase activity detection. Herein, we reported a simple colorimetric strategy for label-free quantification of human telomerase activity in urine by using hemin-graphene nanomaterial (H-GNs). H-GNs possessed tailored dispersibility in the high salt concentration and highly active biomimetic oxidation catalyst property...
September 2, 2016: Biosensors & Bioelectronics
Bum-Yong Kang, Kathy Park, Jennifer M Kleinhenz, Tamara C Murphy, Roy L Sutliff, David Archer, C Michael Hart
Pulmonary hypertension (PH), a serious complication of sickle cell disease (SCD), causes significant morbidity and mortality. While recent study determined that hemin release during hemolysis triggers endothelial dysfunction in SCD, the pathogenesis of SCD-PH remains incompletely defined. This study examines peroxisome proliferator-activated receptor gamma (PPARγ) regulation in SCD-PH and endothelial dysfunction. PH and right ventricular hypertrophy (RVH) were studied in Townes humanized sickle cell (SS) and littermate control (AA) mice...
September 9, 2016: American Journal of Respiratory Cell and Molecular Biology
Kai Shi, Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
Specific and sensitive detection of protein biomarkers is of great importance in biomedical and bioanalytical applications. In this work, a dual amplified signal enhancement approach based on the integration of catalytic hairpin assembly (CHA) and terminal deoxynucleotidyl transferase (TdT)-mediated in situ DNA polymerization has been developed for highly sensitive and label-free electrochemical detection of thrombin in human serums. The presence of the target thrombin leads to the unfolding and capture of a significant number of hairpin signal probes with free 3'-OH termini on the sensor electrode...
August 18, 2016: Biosensors & Bioelectronics
Zhenli Qiu, Jian Shu, Yu He, Zhenzhen Lin, Kangyao Zhang, Shuzhen Lv, Dianping Tang
Lysozyme with a small monomeric globular enzymatic protein is part of the innate immune system, and its deficiency can cause the increased incidence of disease. Herein, we devise a new signal-enhanced fluorescence aptasensing platform for quantitative screening of lysozyme by coupling with rolling circle amplification (RCA) and strand hybridization reaction, accompanying the assembly of CdTe/CdSe quantum dots (QDs) and hemin/G-quadruplex DNzyme. Initially, target-triggered release of the primer was carried out from DNA duplex via the reaction of the aptamer with the analyte, and the released primer could be then utilized as the template to produce numerous repeated oligonucleotide sequences by the RCA reaction...
August 3, 2016: Biosensors & Bioelectronics
Vitul Jain, Manickam Yogavel, Amit Sharma
Excess cellular heme is toxic, and malaria parasites regulate its levels during hemoglobin digestion. Aminoacyl-tRNA synthetases are ubiquitous enzymes, and of these, arginyl-tRNA synthetase (RRS) is unique as its enzymatic product of charged tRNA is required for protein synthesis and degradation. We show that Plasmodium falciparum arginyl-tRNA synthetase (PfRRS) is an active, cytosolic, and monomeric enzyme. Its high-resolution crystal structure highlights critical structural differences with the human enzyme...
September 6, 2016: Structure
Yoshiko Kubota, Kazumi Nomura, Yasutake Katoh, Rina Yamashita, Kiriko Kaneko, Kazumichi Furuyama
In eukaryotic cells, heme production is tightly controlled by heme itself through negative feedback-mediated regulation of nonspecific 5-aminolevulinate synthase (ALAS1), which is a rate-limiting enzyme for heme biosynthesis. However, the mechanism driving the heme-dependent degradation of the ALAS1 protein in mitochondria is largely unknown. In the current study, we provide evidence that the mitochondrial ATP-dependent protease ClpXP, which is a heteromultimer of CLPX and CLPP, is involved in the heme-dependent degradation of ALAS1 in mitochondria...
September 23, 2016: Journal of Biological Chemistry
Mohammed Nooraldeen Al-Qattan, Mohd Nizam Mordi, Sharif Mahsofi Mansor
BACKGROUND: Glutathione-s-transferases (GSTs) are enzymes that principally catalyze the conjugation of electrophilic compounds to the endogenous nucleophilic glutathione substrate, besides, they have other non-catalytic functions. The Plasmodium falciparum genome encodes a single isoform of GST (PfGST) which is involved in buffering the toxic heme, thus considered a potential anti-malarial target. In mammals several classes of GSTs are available, each of various isoforms. The human (human GST Pi-1 or hGSTP1) and mouse (murine GST Mu-1 or mGSTM1) GST isoforms control cellular apoptosis by interaction with signaling proteins, thus considered as potential anti-cancer targets...
July 25, 2016: Computational Biology and Chemistry
Fernanda Duarte, Edson Andrade Pessoa, Luciana Aparecida Reis, Nestor Schor, Fernanda Teixeira Borges
INTRODUCTION: Priming is the mechanism of protection induced by a previous exposition of a cell or organ to low or equal concentrations of a toxic substance. OBJECTIVE: To analyze the mechanism of priming induced by the previous exposition to gentamicin in human proximal tubular cells and nephrotoxic acute renal failure (ARF). METHODS: Wistar rats and tubular cells were exposed to gentamicin 2mM during 24h or 40 mg/kg during 3 days and after one rest week were exposed to the same concentration during 24h in cells and additional ten days in rats...
June 2016: Jornal Brasileiro de Nefrologia: ʹorgão Oficial de Sociedades Brasileira e Latino-Americana de Nefrologia
Jianmei Yang, Baoting Dou, Ruo Yuan, Yun Xiang
Thrombin plays important roles for the diagnosis of neurodegenerative and cardiovascular diseases. By integrating proximity binding-induced strand displacement and metal ion-dependent DNAzyme recycling amplification, we demonstrate here the development of a simple and sensitive strategy for the detection of thrombin in human serums. The binding of the two distinct aptamers to the thrombin targets increases the local concentration of the aptamers and facilitates the release of the enzymatic sequences through proximity binding-induced strand displacement...
August 16, 2016: Analytical Chemistry
Saad Tayyab, Mohamad Mirza Izzudin, Md Zahirul Kabir, Shevin R Feroz, Wei-Ven Tee, Saharuddin B Mohamad, Zazali Alias
Binding characteristics of a promising anticancer drug, axitinib (AXT) to human serum albumin (HSA), the major transport protein in human blood circulation, were studied using fluorescence, UV-vis absorption and circular dichroism (CD) spectroscopy as well as molecular docking analysis. A gradual decrease in the Stern-Volmer quenching constant with increasing temperature revealed the static mode of the protein fluorescence quenching upon AXT addition, thus confirmed AXT-HSA complex formation. This was also confirmed from alteration in the UV-vis spectrum of HSA upon AXT addition...
September 2016: Journal of Photochemistry and Photobiology. B, Biology
Liang Zhu, Maohong Cao, Yaohui Ni, Lijian Han, Aihua Dai, Rongrong Chen, Xiaojin Ning, Xiaorong Liu, Kaifu Ke
Human transforming growth factor β-activated kinase (TAK1)-binding protein 3 (TAB3) is a regulator of NF-κB which has been mainly found in a variety of cancers. While TAB3 is highly expressed in brain tissue, little is known about the function of TAB3 in central nervous system. Our group established an animal ICH model with autologous whole blood injected into brain, and also a cell ICH model with hemin stimulation. Our Western blot result showed up-regulation of TAB3 during neuronal apoptosis in the model of intracerebral hemorrhage (ICH), which was also approved by immunofluorescence and immunohistochemistry result...
June 28, 2016: Cellular and Molecular Neurobiology
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