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https://www.readbyqxmd.com/read/28344061/compartmentation-of-mitochondrial-and-oxidative-metabolism-in-growing-hair-follicles-a-ring-of-fire
#1
John J Lemasters, Venkat K Ramshesh, Gregory L Lovelace, John Lim, Graham D Wright, Duane Harland, Thomas L Dawson
Little is known about the energetics of growing hair follicles, particularly in the mitochondrially abundant bulb. Here, mitochondrial and oxidative metabolism was visualized by multiphoton and light sheet microscopy in cultured bovine hair follicles and plucked human hairs. Mitochondrial membrane potential (ΔΨ), cell viability, reactive oxygen species (ROS) and secretory granules were assessed with parameter-indicating fluorophores. In growing follicles, lower bulb epithelial cells had high viability, and mitochondria were polarized...
March 23, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28338074/self-phase-matched-second-harmonic-and-white-light-generation-in-a-biaxial-zinc-tungstate-single-crystal
#2
Pawel Osewski, Alessandro Belardini, Emilija Petronijevic, Marco Centini, Grigore Leahu, Ryszard Diduszko, Dorota A Pawlak, Concita Sibilia
Second-order nonlinear optical materials are used to generate new frequencies by exploiting second-harmonic generation (SHG), a phenomenon where a nonlinear material generates light at double the optical frequency of the input beam. Maximum SHG is achieved when the pump and the generated waves are in phase, for example through birefringence in uniaxial crystals. However, applying these materials usually requires a complicated cutting procedure to yield a crystal with a particular orientation. Here we demonstrate the first example of phase matching under the normal incidence of SHG in a biaxial monoclinic single crystal of zinc tungstate...
March 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28333369/in-vivo-multiphoton-microscopy-of-picosecond-laser-induced-optical-breakdown-in-human-skin
#3
Mihaela Balu, Griffin Lentsch, Dorota Z Korta, Karsten König, Kristen M Kelly, Bruce J Tromberg, Christopher B Zachary
IMPORTANCE: Improvements in skin appearance resulting from treatment with fractionated picosecond-lasers have been noted, but optimizing the treatment efficacy depends on a thorough understanding of the specific skin response. The development of non-invasive laser imaging techniques in conjunction with laser therapy can potentially provide feedback for guidance and optimizing clinical outcome. OBJECTIVE: The purpose of this study was to demonstrate the capability of multiphoton microscopy (MPM), a high-resolution, label-free imaging technique, to characterize in vivo the skin response to a fractionated non-ablative picosecond-laser treatment...
March 23, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28329012/tracking-the-stochastic-fate-of-cells-of-the-renin-lineage-after-podocyte-depletion-using-multicolor-reporters-and-intravital-imaging
#4
Natalya V Kaverina, Hiroyuki Kadoya, Diana G Eng, Michael E Rusiniak, Maria Luisa S Sequeira-Lopez, R Ariel Gomez, Jeffrey W Pippin, Kenneth W Gross, Janos Peti-Peterdi, Stuart J Shankland
Podocyte depletion plays a major role in focal segmental glomerular sclerosis (FSGS). Because cells of the renin lineage (CoRL) serve as adult podocyte and parietal epithelial cell (PEC) progenitor candidates, we generated Ren1cCre/R26R-ConfettiTG/WT and Ren1dCre/R26R-ConfettiTG/WT mice to determine CoRL clonality during podocyte replacement. Four CoRL reporters (GFP, YFP, RFP, CFP) were restricted to cells in the juxtaglomerular compartment (JGC) at baseline. Following abrupt podocyte depletion in experimental FSGS, all four CoRL reporters were detected in a subset of glomeruli at day 28, where they co-expressed de novo four podocyte proteins (podocin, nephrin, WT-1 and p57) and two glomerular parietal epithelial cell (PEC) proteins (claudin-1, PAX8)...
2017: PloS One
https://www.readbyqxmd.com/read/28327181/soluble-oligomeric-amyloid-%C3%AE-induces-calcium-dyshomeostasis-that-precedes-synapse-loss-in-the-living-mouse-brain
#5
Michal Arbel-Ornath, Eloise Hudry, Josiah R Boivin, Tadafumi Hashimoto, Shuko Takeda, Kishore V Kuchibhotla, Steven Hou, Carli R Lattarulo, Arianna M Belcher, Naomi Shakerdge, Pariss B Trujillo, Alona Muzikansky, Rebecca A Betensky, Bradley T Hyman, Brian J Bacskai
BACKGROUND: Amyloid-β oligomers (oAβ) are thought to mediate neurotoxicity in Alzheimer's disease (AD), and previous studies in AD transgenic mice suggest that calcium dysregulation may contribute to these pathological effects. Even though AD mouse models remain a valuable resource to investigate amyloid neurotoxicity, the concomitant presence of soluble Aβ species, fibrillar Aβ, and fragments of amyloid precursor protein (APP) complicate the interpretation of the phenotypes. METHOD: To explore the specific contribution of soluble oligomeric Aβ (oAβ) to calcium dyshomeostasis and synaptic morphological changes, we acutely exposed the healthy mouse brain, at 3 to 6 months of age, to naturally occurring soluble oligomers and investigated their effect on calcium levels using in vivo multiphoton imaging...
March 21, 2017: Molecular Neurodegeneration
https://www.readbyqxmd.com/read/28320907/a-velocity-map-imaging-study-of-methyl-non-resonant-multiphoton-ionization-from-the-photodissociation-of-ch3i-in-the-a-band
#6
Sonia Marggi Poullain, David V Chicharro, Luis Rubio-Lago, Alberto García-Vela, Luis Bañares
Chemical reaction dynamics and, particularly, photodissociation in the gas phase are generally studied using pump-probe schemes where a first laser pulse induces the process under study and a second one detects the produced fragments. Providing an efficient detection of ro-vibrationally state-selected photofragments, the resonance enhanced multiphoton ionization (REMPI) technique is, without question, the most popular approach used for the probe step, while non-resonant multiphoton ionization (NRMPI) detection of the products is scarce...
April 28, 2017: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/28303272/structural-and-functional-differences-between-aorta-and-vena-cava
#7
Jeffrey M Mattson, Yanhang Zhang
Elastin and collagen fibers are the major load-bearing extracellular matrix (ECM) constituents of the vascular wall. Arteries function differently than veins in the circulatory system, however as a result from several treatment options veins are subjected to sudden elevated arterial pressure. It is thus important to recognize the fundamental structure and function differences between a vein and an artery. Our research compared the relationship between biaxial mechanical function and ECM structure of porcine thoracic aorta and inferior vena cava...
March 17, 2017: Journal of Biomechanical Engineering
https://www.readbyqxmd.com/read/28287581/culture-of-adult-transgenic-zebrafish-retinal-explants-for-live-cell-imaging-by-multiphoton-microscopy
#8
Manuela Lahne, Ryne A Gorsuch, Craig M Nelson, David R Hyde
An endogenous regeneration program is initiated by Müller glia in the adult zebrafish (Danio rerio) retina following neuronal damage and death. The Müller glia re-enter the cell cycle and produce neuronal progenitor cells that undergo subsequent rounds of cell divisions and differentiate into the lost neuronal cell types. Both Müller glia and neuronal progenitor cell nuclei replicate their DNA and undergo mitosis in distinct locations of the retina, i.e. they migrate between the basal Inner Nuclear Layer (INL) and the Outer Nuclear Layer (ONL), respectively, in a process described as Interkinetic Nuclear Migration (INM)...
February 24, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28282180/entanglement-by-path-identity
#9
Mario Krenn, Armin Hochrainer, Mayukh Lahiri, Anton Zeilinger
Quantum entanglement is one of the most prominent features of quantum mechanics and forms the basis of quantum information technologies. Here we present a novel method for the creation of quantum entanglement in multipartite and high-dimensional systems. The two ingredients are (i) superposition of photon pairs with different origins and (ii) aligning photons such that their paths are identical. We explain the experimentally feasible creation of various classes of multiphoton entanglement encoded in polarization as well as in high-dimensional Hilbert spaces-starting only from nonentangled photon pairs...
February 24, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28271554/examination-of-wound-healing-after-curettage-by-multiphoton-tomography-of-human-skin-in-vivo
#10
S Springer, M Zieger, A Böttcher, J Lademann, M Kaatz
BACKGROUND: The multiphoton tomography (MPT) has evolved into a useful tool for the non-invasive investigation of morphological and biophysical characteristics of human skin in vivo. Until now, changes of the skin have been evaluated mainly by using clinical and histological techniques. In this study, the progress of wound healing was investigated by MPT over 3 weeks with a final examination after 24 months. Especially, the collagen degradation, reepithelization and tissue formation were examined...
March 7, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/28270981/label-free-imaging-of-amyloids-using-their-intrinsic-linear-and-nonlinear-optical-properties
#11
Patrik K Johansson, Patrick Koelsch
The optical properties of amyloid fibers are often distinct from those of the source protein in its non-fibrillar form. These differences can be utilized for label-free imaging or characterization of such structures, which is particularly important for understanding amyloid fiber related diseases such as Alzheimer's and Parkinson's disease. We demonstrate that two amyloid forming proteins, insulin and β-lactoglobulin (β-LG), show intrinsic fluorescence with emission spectra that are dependent on the excitation wavelength...
February 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28266862/rapid-and-large-area-characterization-of-exfoliated-black-phosphorus-using-third-harmonic-generation-microscopy
#12
Anton Autere, Christopher R Ryder, Antti Säynätjoki, Lasse Karvonen, Babak Amirsolaimani, Robert A Norwood, Nasser Peyghambarian, Khanh Kieu, Harri Lipsanen, Mark C Hersam, Zhipei Sun
Black phosphorus (BP) is a layered semiconductor that recently has been the subject of intense research due to its novel electrical and optical properties, which compare favorably to those of graphene and the transition metal dichalcogenides. In particular, BP has a direct bandgap that is thickness-dependent and highly anisotropic, making BP an interesting material for nanoscale optical and optoelectronic applications. Here, we present a study of the anisotropic third-harmonic generation (THG) in exfoliated BP using a fast scanning multiphoton characterization method...
March 13, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28255346/precise-spatially-selective-photothermolysis-using-modulated-femtosecond-lasers-and-real-time-multimodal-microscopy-monitoring
#13
Yimei Huang, Harvey Lui, Jianhua Zhao, Zhenguo Wu, Haishan Zeng
The successful application of lasers in the treatment of skin diseases and cosmetic surgery is largely based on the principle of conventional selective photothermolysis which relies strongly on the difference in the absorption between the therapeutic target and its surroundings. However, when the differentiation in absorption is not sufficient, collateral damage would occur due to indiscriminate and nonspecific tissue heating. To deal with such cases, we introduce a novel spatially selective photothermolysis method based on multiphoton absorption in which the radiant energy of a tightly focused near-infrared femtosecond laser beam can be directed spatially by aiming the laser focal point to the target of interest...
2017: Theranostics
https://www.readbyqxmd.com/read/28252388/three-dimensional-segmentation-of-the-ex-vivo-anterior-lamina-cribrosa-from-second-harmonic-imaging-microscopy
#14
Sundaresh Ram, Forest Danford, Stephen Howerton, Jeffrey Rodriguez, Jonathan Vande Geest
The lamina cribrosa (LC) 1 is a connective tissue in the posterior eye with a complex mesh-like trabecular microstructure through which all the retinal ganglion cell axons and central retinal vessels pass. Recent studies have demonstrated that changes in the structure of the LC correlate with glaucomatous damage. Thus, accurate segmentation and reconstruction of the LC is of utmost importance. This paper presents a new automated method for segmenting the microstructure of the anterior LC in the images obtained via multiphoton microscopy using a combination of ideas...
February 23, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28249424/alignment-dependent-ultrafast-electron-nuclear-dynamics-in-molecular-high-order-harmonic-generation
#15
Mu-Zi Li, Guang-Rui Jia, Xue-Bin Bian
We investigated the high-order harmonic generation (HHG) process of diatomic molecular ion H2(+) in non-Born-Oppenheimer approximations (NBOA). The corresponding three-dimensional time-dependent Schrödinger equation is solved with arbitrary alignment angles. It is found that the nuclear motion can lead to spectral modulation of HHG in both the tunneling and multiphoton ionization regimes. The universal redshifts of the whole spectrum are unique in molecular HHG. The spectral width of HHG increases in NBOA...
February 28, 2017: Journal of Chemical Physics
https://www.readbyqxmd.com/read/28241274/two-color-temporal-focusing-multiphoton-excitation-imaging-with-tunable-wavelength-excitation
#16
Chi-Hsiang Lien, Gerald Abrigo, Pei-Hsuan Chen, Fan-Ching Chien
No abstract text is available yet for this article.
February 1, 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28235862/reactive-oxygen-species-producing-myeloid-cells-act-as-a-bone-marrow-niche-for-sterile-inflammation-induced-reactive-granulopoiesis
#17
Haiyan Zhu, Hyun-Jeong Kwak, Peng Liu, Besnik Bajrami, Yuanfu Xu, Shin-Young Park, Cesar Nombela-Arrieta, Subhanjan Mondal, Hiroto Kambara, Hongbo Yu, Li Chai, Leslie E Silberstein, Tao Cheng, Hongbo R Luo
Both microbial infection and sterile inflammation augment bone marrow (BM) neutrophil production, but whether the induced accelerated granulopoiesis is mediated by a common pathway and the nature of such a pathway are poorly defined. We recently established that BM myeloid cell-derived reactive oxygen species (ROS) externally regulate myeloid progenitor proliferation and differentiation in bacteria-elicited emergency granulopoiesis. In this article, we show that BM ROS levels are also elevated during sterile inflammation...
April 1, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28218449/magneto-photoluminescence-based-on-two-photon-excitation-in-lanthanide-doped-up-conversion-crystal-particles
#18
Hengxing Xu, Wei Qin, Mingxing Li, Ting Wu, Bin Hu
Experimental studies on magneto-photoluminescence based on two-photon excitation in up-conversion Y2 O2 S:Er, Yb crystal particles are reported. It is found that the up-conversion photoluminescence generated by two-photon excitation exhibits magnetic field effects at room temperature, leading to a two-photon excitation-induced magneto-photoluminescence, when the two-photon excitation exceeds the critical intensity. By considering the spin selection rule in electronic transitions, it is proposed that spin-antiparallel and spin-parallel transition dipoles with spin mixing are accountable for the observed magneto-photoluminescence...
February 20, 2017: Small
https://www.readbyqxmd.com/read/28218294/correlated-electron-nuclear-dynamics-in-above-threshold-multiphoton-ionization-of-asymmetric-molecule
#19
Zhuo Wang, Min Li, Yueming Zhou, Pengfei Lan, Peixiang Lu
The partition of the photon energy into the subsystems of molecules determines many photon-induced chemical and physical dynamics in laser-molecule interactions. The electron-nuclear energy sharing from multiphoton ionization of molecules has been used to uncover the correlated dynamics of the electron and fragments. However, most previous studies focus on symmetric molecules. Here we study the electron-nuclear energy sharing in strong-field photoionization of HeH(2+) by solving the one-dimensional time-dependent Schrödinger equation (TDSE)...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28215085/peptide-functionalized-gold-nanoparticles-as-a-stimuli-responsive-contrast-medium-in-multiphoton-microscopy
#20
Johan Borglin, Robert Selegård, Daniel Aili, Marica B Ericson
There is a need for biochemical contrast mediators with high signal-to-noise ratios enabling noninvasive biomedical sensing, for example, for neural sensing and protein-protein interactions, in addition to cancer diagnostics. The translational challenge is to develop a biocompatible approach ensuring high biochemical contrast while avoiding a raise of the background signal. We here present a concept where gold nanoparticles (AuNPs) can be utilized as a stimuli responsive contrast medium by chemically triggering their ability to exhibit multiphoton-induced luminescence (MIL) when performing multiphoton laser scanning microscopy (MPM)...
March 8, 2017: Nano Letters
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