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Irreversible electroporation

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https://www.readbyqxmd.com/read/29464927/microbial-lipid-extraction-from-lipomyces-starkeyi-using-irreversible-electroporation
#1
Ahasanul Karim, Abu Yousuf, M Amirul Islam, Yasir H Naif, Che Ku Mohammad Faizal, Md Zahangir Alam, Domenico Pirozzi
The aim of the study was to investigate the feasibility of using irreversible electroporation (EP) as a microbial cell disruption technique to extract intracellular lipid within short time and in an eco-friendly manner. An EP circuit was designed and fabricated to obtain 4 kV with frequency of 100 Hz of square waves. The yeast cells of Lipomyces starkeyi (L. starkeyi) were treated by EP for 2-10 min where the distance between electrodes was maintained at 2, 4 and 6 cm. Colony forming units (CFU) were counted to observe the cell viability under the high voltage electric field...
February 21, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29458955/percutaneous-liver-tumour-ablation-image-guidance-endpoint-assessment-and-quality-control
#2
REVIEW
Robbert S Puijk, Alette H Ruarus, Hester J Scheffer, Laurien G P H Vroomen, Aukje A J M van Tilborg, Jan J J de Vries, Ferco H Berger, Petrousjka M P van den Tol, Martijn R Meijerink
Liver tumour ablation nowadays represents a routine treatment option for patients with primary and secondary liver tumours. Radiofrequency ablation and microwave ablation are the most widely adopted methods, although novel techniques, such as irreversible electroporation, are quickly working their way up. The percutaneous approach is rapidly gaining popularity because of its minimally invasive character, low complication rate, good efficacy rate, and repeatability. However, matched to partial hepatectomy and open ablations, the issue of ablation site recurrences remains unresolved and necessitates further improvement...
February 2018: Canadian Association of Radiologists Journal, Journal L'Association Canadienne des Radiologistes
https://www.readbyqxmd.com/read/29458954/irreversible-electroporation-in-hepatopancreaticobiliary-tumours
#3
A H Ruarus, L G P H Vroomen, R S Puijk, H J Scheffer, B M Zonderhuis, G Kazemier, M P van den Tol, F H Berger, M R Meijerink
Hepatopancreaticobiliary tumours are often diagnosed at an advanced disease stage, in which encasement or invasion of local biliary or vascular structures has already occurred. Irreversible electroporation (IRE) is an image-guided tumour ablation technique that induces cell death by exposing the tumour to high-voltage electrical pulses. The cellular membrane is disrupted, while sparing the extracellular matrix of critical tubular structures. The preservation of tissue integrity makes IRE an attractive treatment option for tumours in the vicinity of vital structures such as splanchnic blood vessels and major bile ducts...
February 2018: Canadian Association of Radiologists Journal, Journal L'Association Canadienne des Radiologistes
https://www.readbyqxmd.com/read/29399927/irreversible-electroporation-ablation-for-atrial-fibrillation
#4
Adam Wojtaszczyk, Guido Caluori, Martin Pešl, Katarina Melajova, Zdeněk Stárek
Atrial fibrillation (AF) is one of the most important problems in modern cardiology. Thermal ablation therapies, especially radiofrequency ablation (RF), are currently "gold standard" to treat symptomatic AF by localized tissue necrosis. Despite the improvements in reestablishing sinus rhythm using available methods, both success rate and safety are limited by the thermal nature of procedures. Thus, while keeping the technique in clinical practice, safer and more versatile methods of removing abnormal tissue are being investigated...
February 5, 2018: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/29396626/-ablative-therapy-of-small-renal-masses
#5
REVIEW
M C Kriegmair, N Wagener, S J Diehl, N Rathmann
Renal cell cancer is nowadays predominantly diagnosed in early stages due to the widespread use of sectional imaging for unrelated symptoms. Small renal masses (<4 cm) feature a largely indolent biology with a very low risk for metastasis or even a benign biology in up to 30% of the cases. Consequently, there is a need for less invasive therapeutic alternatives to nephron-sparing surgery. Meanwhile, there is a broad portfolio of local ablation techniques to treat small renal tumors. These include the extensively studied radiofrequency ablation and cryoablation techniques as well as newer modalities like microwave ablation and irreversible electroporation as more experimental techniques...
February 2, 2018: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/29358001/catheter-based-endobronchial-electroporation-is-feasible-for-the-focal-treatment-of-peribronchial-tumors
#6
Hiroshi Kodama, Laurien G Vroomen, Eisuke Ueshima, Jennifer Reilly, Whitney Brandt, Lee-Ronn Paluch, Sebastien Monette, David Jones, Stephen B Solomon, Govindarajan Srimathveeravalli
OBJECTIVE: To evaluate the feasibility of catheter-based endobronchial electroporation for the treatment of peribronchial tumors and assess the incidence of treatment-related adverse events. METHODS: Cytotoxicity of electroporation with or without cisplatin or gefitinib was assessed in vitro with lung cancer and normal cell lines. A novel catheter was designed for endobronchial electroporation, and computer simulations were used to predict in vivo treatment effects...
December 20, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/29335228/irreversible-electroporation-for-locally-advanced-pancreatic-cancer-through-a-minimally-invasive-surgery-supported-by-laparoscopic-ultrasound
#7
Ernesto Tartaglia, Massimiliano Fabozzi, Antonia Rizzuto, Anna Settembre, Roberta Abete, Ludovica Guerriero, Pasqualino Favoriti, Diego Cuccurullo, Francesco Corcione
INTRODUCTION: Pancreatic cancer is one of the most lethal cancers worldwide, with 5-years survival rate as low as 6%. The majority of pancreatic cancer patients present locally advanced or metastatic disease at diagnosis. Typically, patients affected by locally advanced pancreatic cancer (LAPC) do not undergo radical surgery but are treated with focal ablative therapies. However, a high rate of morbidity due to the heat sink effect has limited the application of ablative techniques on a routine basis in LAPC patients...
December 28, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29333120/focused-transhepatic-electroporation-mediated-by-hypersaline-infusion-through-the-portal-vein-in-rat-model-preliminary-results-on-differential-conductivity
#8
Clara Pañella, Quim Castellví, Xavier Moll, Rita Quesada, Alberto Villanueva, Mar Iglesias, Dolores Naranjo, Patricia Sánchez-Velázquez, Anna Andaluz, Luís Grande, Antoni Ivorra, Fernando Burdío
Background: Spread hepatic tumours are not suitable for treatment either by surgery or conventional ablation methods. The aim of this study was to evaluate feasibility and safety of selectively increasing the healthy hepatic conductivity by the hypersaline infusion (HI) through the portal vein. We hypothesize this will allow simultaneous safe treatment of all nodules by irreversible electroporation (IRE) when applied in a transhepatic fashion. Material and methods: Sprague Dawley (Group A, n = 10) and Athymic rats with implanted hepatic tumour (Group B, n = 8) were employed...
December 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/29307242/technology-of-irreversible-electroporation-and-review-of-its-clinical-data-on-liver-cancers
#9
Emil I Cohen, David Field, George Emmett Lynskey, Alexander Y Kim
Irreversible electroporation (IRE) has developed as a novel percutaneous ablative technique over the past decade and its utility in the treatment of primary and metastatic liver disease has progressed rapidly. Areas Covered: After discussing the principles behind the technology and the practical steps in its use, this article offers a detailed analysis of the recent published work that evaluates its safety and efficacy. The strengths and weaknesses of other ablative techniques, including radiofrequency ablation, microwave ablation and cryoablation, are discussed in detail...
January 8, 2018: Expert Review of Medical Devices
https://www.readbyqxmd.com/read/29305201/initial-assessment-of-the-efficacy-of-irreversible-electroporation-ire-in-the-focal-treatment-of-localised-renal-cell-carcinoma-rcc-with-delayed-interval-kidney-tumour-resection-irene-trial-an-ablate-and-resect-pilot-study
#10
Johann J Wendler, Maciej Pech, Frank Fischbach, Julian Jürgens, Björn Friebe, Daniel Baumunk, Markus Porsch, Simon Blaschke, Daniel Schindele, Sandra Siedentopf, Jens Ricke, Martin Schostak, Jens Köllermann, Uwe B Liehr
OBJECTIVES: To assess the efficacy of irreversible electroporation (IRE) ablation of pT1a renal-cell carcinoma (RCC) in the first prospective, monocentric Phase 2a pilot ablate-and-resect study (IRENE trial). It has been postulated that focal IRE can bring about complete ablation of soft-tissue tumours with protection of healthy peritumoral tissue and anatomical structures. METHODS: The first seven study patients, with biopsy-proven pT1a RCC (15-39mm) underwent IRE...
January 2, 2018: Urology
https://www.readbyqxmd.com/read/29303914/current-opinion-in-urology-2017-focal-therapy-of-small-renal-lesions
#11
Brett A Johnson, Jeffrey A Cadeddu
PURPOSE OF REVIEW: With the increasing incidence of small renal masses (SRMs), ablative technologies are becoming more commonly utilized. With any nascent treatment modality, outcomes literature needs to be constantly re-evaluated. The purpose of this review is to revisit the most updated literature regarding the safety and efficacy of ablative treatments of renal lesions. RECENT FINDINGS: Recent literature demonstrates that small renal tumor ablation is safe and effective...
March 2018: Current Opinion in Urology
https://www.readbyqxmd.com/read/29287522/effect-of-varying-parameters-on-irreversible-electroporation-in-a-porcine-model
#12
Noah E Canvasser, Aaron H Lay, Ersin Koseoglu, Nicholas Kavoussi, Igor Sorokin, Jeffrey C Gahan, Elena Lucas, Jeffrey A Cadeddu
Introduction and Objective Irreversible electroporation is a new ablative technology to treat small renal masses. We evaluated varied ablation settings on lesion size and temperature changes in a porcine model. Methods After Institutional Animal Care and Use Committee approval, 36 laparoscopic-guided and 16 open ablations were performed on 13 domestic female pigs. Ablation parameters studied were voltage (1000 V/cm, 1500 V/cm, or 2000 V/cm), probe exposure (1.0 or 1.5 cm), and lesion size over time (survival) (0-day, 7-day, or 14-day)...
December 29, 2017: Journal of Endourology
https://www.readbyqxmd.com/read/29277585/a-comparison-of-time-taken-to-return-to-baseline-erectile-function-following-focal-and-whole-gland-ablative-therapies-for-localized-prostate-cancer-a-systematic-review
#13
REVIEW
Nicholas A Faure Walker, Joseph M Norris, Taimur T Shah, Tet Yap, Paul Cathcart, Caroline M Moore, Hashim U Ahmed, Mark Emberton, Suks Minhas
OBJECTIVES: To systematically review erectile function (EF) outcomes following primary whole gland (WG) and focal ablative therapies for localized prostate cancer to ascertain whether the treatment modality or intended treatment volume affects the time taken to recover baseline EF. METHOD AND MATERIALS: A systematic review was performed according to the preferred reporting items for systematic review and meta-analysis statement. Inclusion criteria were men with localized prostate cancer treated with primary, ablative therapy...
February 2018: Urologic Oncology
https://www.readbyqxmd.com/read/29235992/avoiding-neuromuscular-stimulation-in-liver-irreversible-electroporation-using-radiofrequency-electric-fields
#14
Quim Castellví, Borja Mercadal, Xavier Moll, Dolors Fondevila, Anna Andaluz, Antoni Ivorra
Electroporation based treatments typically consist in applying high voltage dc pulses. As an undesired side effect, these dc pulses cause electrical stimulation of excitable tissues such as motor nerves. In the present in vivo study, it was explored the use of bursts of sinusoidal voltage in the frequency range from 50 kHz to 2 MHz to induce irreversible electroporation (IRE) whilst avoiding neuromuscular stimulation. Series of 100 dc pulses or sinusoidal bursts, both with an individual duration of 100 µs, were delivered to rabbit liver through thin needles in a monopolar electrode configuration and thoracic movements were recorded with an accelerometer...
December 13, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/29232270/novel-focal-therapy-treatment-options-for-prostate-cancer
#15
Ryan P Werntz, Scott E Eggener
PURPOSE OF REVIEW: Examine and discuss novel focal therapy treatment options for prostate cancer. RECENT FINDINGS: With the widespread adoption of mpMRI-guided biopsies for prostate cancer, use of image-guided treatment of prostate cancer has increased. Focal therapies leading to partial gland ablation such as partial prostatectomy, focal laser ablation, irreversible electroporation, vascular targeted photodynamic therapy, and focal radiofrequency ablation have emerged and begun to be properly evaluated...
December 9, 2017: Current Opinion in Urology
https://www.readbyqxmd.com/read/29232185/18f-fdg-pet-biomarkers-help-detect-early-metabolic-response-to-irreversible-electroporation-and-predict-therapeutic-outcomes-in-a-rat-liver-tumor-model
#16
Xifu Wang, Zhanliang Su, Tianchu Lyu, Matteo Figini, Daniel Procissi, Junjie Shangguan, Chong Sun, Bin Wang, Na Shang, Shanzhi Gu, Quanhong Ma, Andrew C Gordon, Kai Lin, Jian Wang, Robert J Lewandowski, Riad Salem, Vahid Yaghmai, Andrew C Larson, Zhuoli Zhang
Purpose To test the hypothesis that biomarkers of fluorine 18 (18F) fluorodeoxyglucose (FDG) positron emission tomography (PET) can be used for the early detection of therapeutic response to irreversible electroporation (IRE) of liver tumor in a rodent liver tumor model. Materials and Methods The institutional animal care and use committee approved this study. Rats were inoculated with McA-RH7777 liver tumor cells in the left median and left lateral lobes. Tumors were allowed to grow for 7 days to reach a size typically at least 5 mm in longest diameter, as verified with magnetic resonance (MR) imaging...
December 12, 2017: Radiology
https://www.readbyqxmd.com/read/29186222/high-frequency-irreversible-electroporation-targets-resilient-tumor-initiating-cells-in-ovarian-cancer
#17
A Rolong, E M Schmelz, R V Davalos
We explored the use of irreversible electroporation (IRE) and high-frequency irreversible electroporation (H-FIRE) to induce cell death of tumor-initiating cells using a mouse ovarian surface epithelial (MOSE) cancer model. Tumor-initiating cells (TICs) can be successfully destroyed using pulsed electric field parameters common to irreversible electroporation protocols. Additionally, high-frequency pulses seem to induce cell death of TICs at significantly lower electric fields suggesting H-FIRE can be used to selectively target TICs and malignant late-stage cells while sparing the non-malignant cells in the surrounding tissue...
November 29, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/29153290/locally-advanced-pancreatic-cancer-an-emerging-entity
#18
REVIEW
Grainne M O Kane, Jennifer J Knox
Pancreatic adenocarcinoma (PDAC) remains a highly fatal disease that is increasing in incidence. PDAC can be classified according to resectability status with 3 nonmetastatic groups defined: resectable, borderline resectable, and locally advanced PDAC (LAPC). Delineating these subtypes is important with the optimal treatment approach dictated by high-quality CT imaging and multidisciplinary team discussion. Patients with LAPC are thought unresectable and are therefore rarely cured. In these patients, chemotherapy remains the mainstay of treatment...
November 16, 2017: Current Problems in Cancer
https://www.readbyqxmd.com/read/29143247/using-circulating-tumor-cells-to-evaluate-the-efficacy-of-irreversible-electroporation-for-unresectasble-pancreatic-cancer
#19
Mao Lin, Mohammed Alnaggar, Shu-Zhen Liang, Jian Shi, Li-Zhi Niu, Ji-Bing Chen, Ke-Cheng Xu
We used circulating tumor cells (CTCs) as biomarkers to evaluate the efficacy of pre-irreversible electroporation (IRE) and post-IRE for unresectable pancreatic cancer (PC). Real-time qPCR was used to detect potential biomarker genes in CTCs, and magnetic-activated cell sorting (MACS) and fluorescence-activated cell sorting (FACS) were performed on 43 patients with PC who underwent IRE. Some patients experienced adverse reactions within 30 days of the operation, including arrhythmia (6.9%), intraoperative transient change of blood pressure (25...
November 15, 2017: Immunologic Research
https://www.readbyqxmd.com/read/29143077/calcein-release-from-cells-in-vitro-via-reversible-and-irreversible-electroporation
#20
Violeta Rajeckaitė, Baltramiejus Jakštys, Arnas Rafanavičius, Martynas Maciulevičius, Milda Jakutavičiūtė, Saulius Šatkauskas
The aim of this study was to investigate the dependence of calcein extraction and cell viability on the parameters of pulsed electric field (PEF). Two different approaches concerning PEF parameters were investigated: (1) extraction efficiency and cell viability dependence on pulse number, exploiting 1200 V/cm 100 µs duration high voltage (HV) electric pulses and (2) extraction efficiency and cell viability dependence on the pulses with different duration (44-400 µs) and electric field strength (600-1800 V/cm) that result in the same amount of electric field energy delivered to Chinese hamster ovary cells...
November 15, 2017: Journal of Membrane Biology
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