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https://www.readbyqxmd.com/read/28527350/bi-dimensional-variational-mode-decomposition-of-laser-speckle-contrast-imaging-data-a-clinical-approach-to-critical-limb-ischemia
#1
Anne Humeau-Heurtier, Pierre Abraham, Samir Henni
The number of patients suffering from peripheral arterial occlusive disease (PAOD) is growing worldwide. PAOD corresponds to an impairment of perfusion in the distal portion of some arteries that can generate critical limb ischemia. For some patients, the pathology leads to the necessity of amputation. PAOD may be diagnosed and studied with transcutaneous oxygen (tcpO2) measurements. However, the tcpO2 technique is a contact technique: probes have to be positioned on the limb, which may be impossible in case of ulcers and can lead to infection risks...
May 13, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28527269/-first-evidence-of-lymphocytic-choriomeningitis-virus-arenavirus-infection-in-mus-musculus-rodents-captured-in-the-urban-area-of-the-municipality-of-sincelejo-sucre-colombia
#2
Anais Castellar, Marco Guevara, Juan D Rodas, Andrés F Londoño, Esteban Arroyave, Francisco J Díaz, Silvana Levis, Pedro J Blanco
INTRODUCTION: The lymphocytic choriomeningitis virus is an Old World arenavirus that infects Mus musculus, and can cause congenital hydrocephalus, chorioretinitis and multisystemic failure in transplant human recipients. Although the disease has not been clinically diagnosed in Colombia yet, there have been reports of infection with the Pichindé virus in rodents from Cauca and Valle del Cauca departments, and with the Guanarito virus in rodents from Córdoba department. OBJECTIVE: To identify the lymphocytic choriomeningitis virus from Mus musculus captured in the municipality of Sincelejo...
April 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527257/-polyclonal-antibodies-against-recombinant-dengue-virus-ns3-protein
#3
Liliana Morales, Myriam L Velandia, María Angélica Calderon, Jaime E Castellanos, Jacqueline Chaparro-Olaya
INTRODUCTION: Dengue is a disease caused by one of four serotypes of the dengue virus (DENV) and is endemic in approximately 130 countries. The incidence of dengue has increased dramatically in recent decades, as well as the frequency and magnitude of outbreaks. Despite all efforts, there are no prophylactic or therapeutic treatments for the disease. Accordingly, research on the processes governing the DENV infection cycle is essential to develop vaccines or antiviral therapies. One of themost attractive DENV molecules to investigate is nonstructural protein 3 (NS3), which is essential for viral replication and a major immune target for infection...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527251/-pathological-findings-in-patients-with-hiv-infection-and-lymphadenopathies
#4
Federico Rodríguez-Vega, Miguel Botero, Jorge Alberto Cortés, Ángela Tobón
INTRODUCTION: Lymphadenopathy is a frequent clinical finding in HIV-infected patients. The differential diagnosis includes infection, malignancy or reactive changes. Currently, there are no data on this topic in the region. OBJECTIVES: To describe the etiology of lymph node pathology in HIV-infected patients from the Hospital La María in Medellín, Colombia. MATERIALS AND METHODS: The medical records of HIV-infected patients with lymphadenopathy who underwent excisional lymph node biopsy between June 2009 and October 2011 were retrospectively evaluated...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527250/-risk-of-transmission-of-chagas-disease-by-intrusion-of-triatomines-and-wild-mammals-in-bucaramanga-santander-colombia
#5
Marlene Reyes, Ángela Torres, Lyda Esteban, Mónica Flórez, Víctor Manuel Angulo
INTRODUCTION: Notice of triatomines in dwellings of some neighborhoods in Bucaramanga motivated the realization of this study.Objetive: To evaluate the intrusion of triatomines and mammals, as well as some risk factors in urban dwellings. MATERIALS AND METHODS: Triatomines were collected in a neighborhood in Bucaramanga, Santander, on a monthly basis during one year with participation of the community. Collection included manual search in lamp posts, use of light traps, animal bait, and chemical attractants in nearby forests...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527248/-clinical-and-demographic-profile-and-risk-factors-for-clostridium-difficile-infection
#6
Carlos Carvajal, Carlos Pacheco, Fabián Jaimes
INTRODUCTION: Clostridium difficile infection is the leading cause of nosocomial infectious diarrhea. The increasing incidence added to a lower rate of response to the initial treatment and higher rates of relapse has generated a higher burden of the disease. OBJECTIVE: To determine the clinical characteristics of hospitalized patients with C. difficile infection. MATERIALS AND METHODS: We made a nested case-cohort study. We reviewed medical records of the patients with nosocomial diarrhea for whom an assay for toxin A-B of C...
January 24, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28527200/contrast-enhanced-ultrasound-ceus-in-renal-imaging-at-an-interdisciplinary-ultrasound-centre-possibilities-of-dynamic-microvascularisation-and-perfusion
#7
Franz Josef Putz, Anna Erlmeier, Isabel Wiesinger, Niklas Verloh, Christian Stroszczynski, Bernhard Banas, Ernst Michael Jung
OBJECTIVE: To identify the indications for CEUS in renal imaging in an interdisciplinary ultrasound department. METHODS: 102 CEUS examinations of the kidney in 82 patients between September 2014 and July 2016 were analysed regarding the indication for ultrasound. CEUS was performed by one experienced sonographer agent after bolus injection of 1.0 up to 2.4 ml sulphur hexafluoride microbubbles using multifrequency probes with Contrast Harmonic imaging. RESULTS: CEUS of the kidney was performed in patients from 20 to 87 years...
May 19, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28527195/changes-in-sternal-perfusion-following-internal-mammary-artery-bypass-surgery
#8
Christian D Taeger, Konstantin Klein, Raymund E Horch, Michael Weyand, Justus P Beier
BACKROUND: Coronary bypass surgery using the internal mammary artery (IMA) is among the most commonly performed procedures in treatment of advanced coronary vessel disease. Further, bilateral harvesting of the IMA is associated with increased rates of sternal wound infections. OBJECTIVE: This study aimed to explore changes in sternal perfusion, following left internal mammary artery (LIMA) harvesting. METHODS: 60 patients were divided equally into intervention- and control cohorts and underwent assessment of oxygen saturation (sO2) and relative blood flow (RBF) by laser doppler flowmetry remission spectroscopy preoperatively, 24 h and 72 h postoperatively...
May 18, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28526988/identification-of-candidate-genes-for-paratuberculosis-resistance-in-the-native-italian-garfagnina-goat-breed
#9
Francesca Cecchi, Claudia Russo, Daniela Iamartino, Alessia Galiero, Barbara Turchi, Filippo Fratini, Sara Degl'Innocenti, Raffaele Mazza, Stefano Biffani, Giovanna Preziuso, Carlo Cantile
Paratuberculosis disease is a chronic bacterial disease infection of ruminants of global relevance, caused by MAP (Mycobacterium avium subsp. paratuberculosis). The present study was conducted on the Garfagnina goat breed that is an Italian native goat population registered on the Tuscan regional repertory of genetic resources at risk of extinction. Forty-eight adult goats (27 serologically positive to MAP-positive and 21 serologically negative to MAP-negative) belonging to a single flock that had experienced annual mortalities due to MAP infection were identified and genotyped with the Illumina GoatSNP60 BeadChip...
May 19, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28526972/patient-experiences-attitudes-and-expectations-towards-receiving-information-about-anti-tnf-medication-a-quantitative-study
#10
Jon Packham, Paul Arkell, Tom Sheeran, Ann Brownfield, Anthony Cadwgan, Sarah Ryan
The objective of the study was to measure patient attitudes and experience of information received during drug counselling for rheumatoid arthritis (RA) medications. This is a cross-sectional UK postal questionnaire study. Three RA patient groups-disease-modifying antirheumatic drugs (DMARDs) only, first anti-tumour necrosis factor (anti-TNF) and failed anti-TNF-were sent postal questionnaires. Data on patient history/demographics, drug counselling experience, knowledge of drug side effects, attitudes to vaccinations, cancer screening and blood borne virus testing was collected; 264/679 (39%) patients responded (median age 65 years, 66% female, median disease duration 15 years)...
May 19, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28526937/molecular-confirmation-of-intraspecific-tomato-solanum-lycopersicum-hybrids-and-their-evaluation-against-late-blight-and-cucumber-mosaic-virus
#11
Amjad Hameed, Muhammad Yussouf Saleem, Khalid Pervaiz Akhtar, Muhammad Shoaib, Qumer Iqbal, Muhammad Asghar
Tomato is one of the most consumed vegetables in the world. Diseases are the number one concern in the development of high-yield and disease-resistant tomato hybrids which is the foremost priority of breeders. Present study was conducted (1) to develop DNA-based markers for genetic confirmation of tomato F1 hybrids, (2) to utilize sequenced characterized amplified region (SCAR) marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato and (3) to evaluate male and female parental genotypes and their F1 hybrids against late blight (LB) and cucumber mosaic virus (CMV)...
May 19, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28526906/mycobacterium-chelonae-infection-of-the-buttocks-secondary-to-lipofilling-a-case-report-and-review-of-the-literature
#12
Sarah E Hammond, Ahmed Al-Bayati, Natalie Joumblat, Christopher J Salgado
Mycobacterium chelonae is a nontuberculous mycobacterium, classified as a Runyon type IV mycobacterium. In relation to humans, it is most commonly associated with tissue trauma or pulmonary infections. The majority of medical reports describe finding M. chelonae in the surgical setting, attributing infection to inadequate sterilization of surgical equipment. Symptoms are often nonspecific and include pain, erythema, and draining subcutaneous nodules and skin lesions. Therefore, the diagnosis of M. chelonae is often difficult to establish without prior suspicion of the disease, but can be confirmed with culture...
May 19, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28526790/post-epizootic-persistence-of-asymptomatic-mycoplasma-conjunctivae-infection-in-iberian-ibex
#13
Xavier Fernández-Aguilar, Oscar Cabezón, José Enrique Granados, Joachim Frey, Emmanuel Serrano, Roser Velarde, Francisco Javier Cano-Manuel, Gregorio Mentaberre, Arián Ráez-Bravo, Paulino Fandos, Jorge Ramón López-Olvera
The susceptibility of Iberian ibex (Capra pyrenaica) to Mycoplasma conjunctivae ocular infection and the changes in their interaction over time were studied in terms of clinical outcome, molecular detection and IgG immune response in a captive population that underwent a severe infectious keratoconjunctivitis (IKC) outbreak. Mycoplasma conjunctivae was detected for the first time in the Iberian ibexes coinciding with the IKC outbreak. Its prevalence had a decreasing trend in 2013 that was consistent with the clinical resolution (August 35...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526665/the-sensor-study-protocol-for-a-mixed-methods-study-of-self-management-checks-to-predict-exacerbations-of-pseudomonas-aeruginosa-in-patients-with-long-term-respiratory-conditions
#14
Claire Roberts, Thomas L Jones, Samal Gunatilake, Will Storrar, Scott Elliott, Sharon Glaysher, Ben Green, Steven Rule, Carole Fogg, Ann Dewey, Kevin A Auton, Anoop J Chauhan
BACKGROUND: There are an estimated three million people in the United Kingdom with chronic obstructive pulmonary disease (COPD), and the incidence of bronchiectasis is estimated at around 0.1% but is more common in COPD and severe asthma. Both COPD and bronchiectasis are characterized by exacerbations in which bacteria play a central role. Pseudomonas aeruginosa is isolated from sputum samples from 4% to 15% of adults with COPD and is more likely to be isolated from patients with severe disease...
May 19, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28526661/the-parasol-protocol-an-implementation-science-study-of-hiv-continuum-of-care-interventions-for-gay-men-and-transgender-women-in-burma-myanmar
#15
Andrea L Wirtz, Soe Naing, Emily Clouse, Kaung Htet Thu, Sandra Hsu Hnin Mon, Zin Min Tun, Stefan Baral, Aung Zayar Paing, Chris Beyrer
BACKGROUND: Efforts to improve HIV diagnosis and antiretroviral therapy (ART) initiation among people living with HIV and reduce onward transmission of HIV rely on innovative interventions along multiple steps of the HIV care continuum. These innovative methods are particularly important for key populations, including men who have sex with men (MSM) and transgender women (TW). The HIV epidemic in Myanmar is concentrated among key populations, and national efforts now focus on reducing stigma and improving engagement of MSM and TW in HIV prevention and care...
May 17, 2017: JMIR Research Protocols
https://www.readbyqxmd.com/read/28526608/microrna-analysis-of-taenia-crassiceps-cysticerci-under-praziquantel-treatment-and-genome-wide-identification-of-taenia-solium-mirnas
#16
Matías Gastón Pérez, Natalia Macchiaroli, Gabriel Lichtenstein, Gabriela Conti, Sebastián Asurmendi, Diego Humberto Milone, Georgina Stegmayer, Laura Kamenetzky, Marcela Cucher, Mara Cecilia Rosenzvit
MicroRNAs (miRNAs) are small non-coding RNAs that have emerged as important regulators of gene expression and perform critical functions in development and disease. In spite of the increased interest in miRNAs from helminth parasites, no information is available on miRNAs from Taenia solium, the causative agent of cysticercosis, a neglected disease affecting millions of people worldwide. Here we performed a comprehensive analysis of miRNAs from Taenia crassiceps, a laboratory model for T. solium studies, and identified miRNAs in the T...
May 16, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526605/microevolutionary-response-of-a-gut-nematode-to-intestinal-inflammation
#17
Cédric Lippens, Bruno Faivre, Gabriele Sorci
Parasitic helminths interfere with the immune responses of their hosts to establish long-lasting, chronic infections. While favorable to the parasite, the capacity to dampen the immune response can also provide a benefit to the host in terms of reduced risk of immune disorders and immunopathology. The immunomodulatory role of nematodes has been exploited in clinical trials to treat a number of inflammatory and immune diseases. However, how parasites adapt to an inflammatory environment remains a poorly explored question...
May 16, 2017: International Journal for Parasitology
https://www.readbyqxmd.com/read/28526582/mait-cells-a-tailor-made-mate-in-the-ancient-battle-against-infectious-diseases
#18
REVIEW
Marcela de Lima Moreira, Moriya Tsuji, Alexandra Jane Corbett, Márcio Sobreira Silva Araújo, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho, Vanessa Peruhype-Magalhães, Jordana Grazziela Coelho-Dos-Reis
It has been almost two decades since the discovery of mucosal-associated invariant T (MAIT)-cells. Several advances in the field have been made such as the discovery of the antimicrobial activity of MAIT-cells, the abundance of these cells in human mucosa and in liver and the discovery of ligands able to bind MR1 and activate MAIT-cells. MAIT-cells are a unique subset of innate-like T-cells that express a canonical T-cell receptor with the alpha chain containing hAV7S2 and AJ33 in humans (TCRVα7.2Jα33) and respond to bacterial/fungus vitamin B2 metabolites by an MR1-dependent pathway...
May 16, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28526564/direct-detection-of-anaplasma-phagocytophilum-by-polymerase-chain-reaction-followed-by-electrospray-ionization-mass-spectrometry-from-human-blood
#19
Heimo Lagler, Nicole Harrison, Manuel Kussmann, Markus Obermüller, Heinz Burgmann, Athanasios Makristathis, Michael Ramharter
Bacterial pathogens not detectable in commercial blood culture assays constitute an important challenge for infectious disease physicians, in particular if clinical symptoms of the illness are unspecific. In this report Anaplasma phagocytophilum was detected directly from a peripheral blood sample of a febrile patient reporting a tick bite by a commercial system based on polymerase chain reaction followed by electrospray ionization mass spectrometry. Therefore, the diagnosis of human granulocytic anaplasmosis infection was established by this diagnostic methodology for the first time...
May 16, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28526563/live-cell-imaging-and-analysis-of-lipid-droplets-biogenesis-in-hcv-infected-cells
#20
Inbar Nevo-Yassaf, Marcos Lovelle, Yaacov Nahmias, Koret Hirschberg, Ella H Sklan
Lipid droplets (LDs) are regulated neutral lipid storage organelles having a central role in numerous cellular processes as well as in various pathologies such as metabolic disorders, immune responses and during pathogen infection. Several viruses were found to use the interface between the endoplasmic reticulum (ER) and LDs as the site for replication and assembly. Due to the growing significance of LDs, extensive efforts are made to study the mechanism and the dynamics of their formation and life history and how are these diverted or modified by pathogens...
May 16, 2017: Methods: a Companion to Methods in Enzymology
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