Lu Wang, Kangjie Shen, Zixu Gao, Ming Ren, Chenlu Wei, Yang Yang, Yinlam Li, Yu Zhu, Simin Zhang, Yiteng Ding, Tianyi Zhang, Jianrui Li, Ming Zhu, Shaoluan Zheng, Yanwen Yang, Shisuo Du, Chuanyuan Wei, Jianying Gu
Radiotherapy (RT) can induce tumor regression outside the irradiation field, known as the abscopal effect. However, the detailed underlying mechanisms remain largely unknown. A tumor-bearing mouse model is successfully constructed by inducing both subcutaneous tumors and lung metastases. Single-cell RNA sequencing, immunofluorescence, and flow cytometry are performed to explore the regulation of tumor microenvironment (TME) by RT. A series of in vitro assays, including luciferase reporter, RNA Pulldown, and fluorescent in situ hybridization (FISH) assays, are performed to evaluate the detailed mechanism of the abscopal effect...
January 29, 2024: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)