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https://www.readbyqxmd.com/read/28938097/effect-of-sos-induced-levels-of-imuabc-on-spontaneous-and-damage-induced-mutagenesis-in-caulobacter-crescentus
#1
Ingrid R Alves, Marco A Lima-Noronha, Larissa G Silva, Frank S Fernández-Silva, Aline Luiza D Freitas, Marilis V Marques, Rodrigo S Galhardo
imuABC (imuAB dnaE2) genes are responsible for SOS-mutagenesis in Caulobacter crescentus and other bacterial species devoid of umuDC. In this work, we have constructed operator-constitutive mutants of the imuABC operon. We used this genetic tool to investigate the effect of SOS-induced levels of these genes upon both spontaneous and damage-induced mutagenesis. We showed that constitutive expression of imuABC does not increase spontaneous or damage-induced mutagenesis, nor increases cellular resistance to DNA-damaging agents...
September 14, 2017: DNA Repair
https://www.readbyqxmd.com/read/28911105/a-novel-nucleoid-associated-protein-coordinates-chromosome-replication-and-chromosome-partition
#2
James A Taylor, Gaël Panis, Patrick H Viollier, Gregory T Marczynski
We searched for regulators of chromosome replication in the cell cycle model Caulobacter crescentus and found a novel DNA-binding protein (GapR) that selectively aids the initiation of chromosome replication and the initial steps of chromosome partitioning. The protein binds the chromosome origin of replication (Cori) and has higher-affinity binding to mutated Cori-DNA that increases Cori-plasmid replication in vivo. gapR gene expression is essential for normal rapid growth and sufficient GapR levels are required for the correct timing of chromosome replication...
September 6, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28886080/stalk-formation-of-brevundimonas-and-how-it-compares-to-caulobacter-crescentus
#3
Patrick D Curtis
The Caulobacter crescentus cell extension known as a stalk represents an unusual bacterial morphology. C. crescentus produces stalks under multiple nutrient conditions, but the length of the stalk is increased in response to phosphate starvation. However, the exact function of the stalk is not known, nor is it known how much stalk biogenesis or function is conserved with other stalked bacteria. Work presented here shows that many organisms in the Caulobacter genus and the next closest genus (Brevundimonas) generally do not synthesize stalks in the relatively-rich PYE growth medium, suggesting that the synthesis of a stalk under nutrient-rich conditions by C...
2017: PloS One
https://www.readbyqxmd.com/read/28886022/ftsex-mediated-regulation-of-the-final-stages-of-cell-division-reveals-morphogenetic-plasticity-in-caulobacter-crescentus
#4
Elizabeth L Meier, Allison K Daitch, Qing Yao, Anant Bhargava, Grant J Jensen, Erin D Goley
During its life cycle, Caulobacter crescentus undergoes a series of coordinated shape changes, including generation of a polar stalk and reshaping of the cell envelope to produce new daughter cells through the process of cytokinesis. The mechanisms by which these morphogenetic processes are coordinated in time and space remain largely unknown. Here we demonstrate that the conserved division complex FtsEX controls both the early and late stages of cytokinesis in C. crescentus, namely initiation of constriction and final cell separation...
September 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28866999/caulobacter-hibisci-sp-nov-isolated-from-rhizosphere-of-hibiscus-syriacus-l-mugunghwa-flower
#5
Gabriela Moya, Zheng-Fei Yan, KyungHwa Won, Jung-Eun Yang, Qi-Jun Wang, MooChang Kook, Tae-Hoo Yi
A Gram-stain-negative, smooth, bright yellow-pigmented, aerobic, catalase- and oxidase-positive and rod-shaped bacterial strain was isolated from rhizosphere of Hibiscus syriacus L. (Mugunghwa flower) located in Kyung Hee University, Yongin, Gyeonggi, South Korea. Cells were dimorphic, non-motile or non-stalked, and motile by means of peritrichous flagellum. The strain, named THG-AG3.4T, grew at 15-35 °C, at pH 6.5-9.0 and in the presence of 0-1.5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain THG-AG3...
September 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28854358/smc-progressively-aligns-chromosomal-arms-in-caulobacter-crescentus-but-is-antagonized-by-convergent-transcription
#6
Ngat T Tran, Michael T Laub, Tung B K Le
The structural maintenance of chromosomes (SMC) complex plays an important role in chromosome organization and segregation in most living organisms. In Caulobacter crescentus, SMC is required to align the left and the right arms of the chromosome that run in parallel down the long axis of the cell. However, the mechanism of SMC-mediated alignment of chromosomal arms remains elusive. Here, using genome-wide methods and microscopy of single cells, we show that Caulobacter SMC is recruited to the centromeric parS site and that SMC-mediated arm alignment depends on the chromosome-partitioning protein ParB...
August 29, 2017: Cell Reports
https://www.readbyqxmd.com/read/28833791/lytm-factors-affect-the-recruitment-of-autolysins-to-the-cell-division-site-in-caulobacter-crescentus
#7
Aleksandra Zielińska, Maria Billini, Andrea Möll, Katharina Kremer, Ariane Briegel, Adrian Izquierdo Martinez, Grant J Jensen, Martin Thanbichler
Most bacteria possess a peptidoglycan cell wall that determines their morphology and provides mechanical robustness during osmotic challenges. The biosynthesis of this structure is achieved by a large set of synthetic and lytic enzymes with varying substrate specificities. Although the biochemical functions of these proteins are conserved and well-investigated, the precise roles of individual factors and the regulatory mechanisms coordinating their activities in time and space remain incompletely understood...
August 23, 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28827812/crosstalk-between-the-tricarboxylic-acid-cycle-and-peptidoglycan-synthesis-in-caulobacter-crescentus-through-the-homeostatic-control-of-%C3%AE-ketoglutarate
#8
Irnov Irnov, Zhe Wang, Nicholas D Jannetty, Julian A Bustamante, Kyu Y Rhee, Christine Jacobs-Wagner
To achieve robust replication, bacteria must integrate cellular metabolism and cell wall growth. While these two processes have been well characterized, the nature and extent of cross-regulation between them is not well understood. Here, using classical genetics, CRISPRi, metabolomics, transcriptomics and chemical complementation approaches, we show that a loss of the master regulator Hfq in Caulobacter crescentus alters central metabolism and results in cell shape defects in a nutrient-dependent manner. We demonstrate that the cell morphology phenotype in the hfq deletion mutant is attributable to a disruption of α-ketoglutarate (KG) homeostasis...
August 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28799725/engineering-of-corynebacterium-glutamicum-for-consolidated-conversion-of-hemicellulosic-biomass-into-xylonic-acid
#9
Sung Sun Yim, Jae Woong Choi, Se Hwa Lee, Eun Jung Jeon, Wook-Jin Chung, Ki Jun Jeong
Xylonic acid is a promising platform chemical with various applications in the fields of food, pharmaceuticals, and agriculture. However, in the current process, xylonic acid is mainly produced by the conversion of xylose, whose preparation requires substantial cost and time. Here, we engineered Corynebacterium glutamicum for the consolidated bioconversion of hemicellulosic biomass (xylan) into xylonic acid in a single cultivation. First, for the efficient conversion of xylose to xylonic acid, xylose dehydrogenase (Xdh) and xylonolactonase (XylC) from Caulobacter crescentus were evaluated together with a previously optimized xylose transporter module (XylE of Escherichia coli), and cells expressing xdh and xylE genes with an optimized expression system could produce xylonic acid from xylose with 100% conversion yield...
August 11, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28768811/conformational-and-chemical-selection-by-a-trans-acting-editing-domain
#10
Eric M Danhart, Marina Bakhtina, William A Cantara, Alexandra B Kuzmishin, Xiao Ma, Brianne L Sanford, Marija Košutić, Yuki Goto, Hiroaki Suga, Kotaro Nakanishi, Ronald Micura, Mark P Foster, Karin Musier-Forsyth
Molecular sieves ensure proper pairing of tRNAs and amino acids during aminoacyl-tRNA biosynthesis, thereby avoiding detrimental effects of mistranslation on cell growth and viability. Mischarging errors are often corrected through the activity of specialized editing domains present in some aminoacyl-tRNA synthetases or via single-domain trans-editing proteins. ProXp-ala is a ubiquitous trans-editing enzyme that edits Ala-tRNA(Pro), the product of Ala mischarging by prolyl-tRNA synthetase, although the structural basis for discrimination between correctly charged Pro-tRNA(Pro) and mischarged Ala-tRNA(Ala) is unclear...
August 15, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28737755/biphasic-growth-dynamics-control-cell-division-in-caulobacter-crescentus
#11
Shiladitya Banerjee, Klevin Lo, Matthew K Daddysman, Alan Selewa, Thomas Kuntz, Aaron R Dinner, Norbert F Scherer
Cell size is specific to each species and impacts cell function. Various phenomenological models for cell size regulation have been proposed, but recent work in bacteria has suggested an 'adder' model, in which a cell increments its size by a constant amount between each division. However, the coupling between cell size, shape and constriction remains poorly understood. Here, we investigate size control and the cell cycle dependence of bacterial growth using multigenerational cell growth and shape data for single Caulobacter crescentus cells...
July 24, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/28697666/bacterial-cell-division-nonmodels-poised-to-take-the-spotlight
#12
Prahathees J Eswara, Kumaran S Ramamurthi
The last three decades have witnessed an explosion of discoveries about the mechanistic details of binary fission in model bacteria such as Escherichia coli, Bacillus subtilis, and Caulobacter crescentus. This was made possible not only by advances in microscopy that helped answer questions about cell biology but also by clever genetic manipulations that directly and easily tested specific hypotheses. More recently, research using understudied organisms, or nonmodel systems, has revealed several alternate mechanistic strategies that bacteria use to divide and propagate...
July 11, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28661661/caulobacter-crescentus-cell-cycle-regulated-dna-methyltransferase-uses-a-novel-mechanism-for-substrate-recognition
#13
COMPARATIVE STUDY
Clayton B Woodcock, Aziz B Yakubov, Norbert O Reich
Caulobacter crescentus relies on DNA methylation by the cell cycle-regulated methyltransferase (CcrM) in addition to key transcription factors to control the cell cycle and direct cellular differentiation. CcrM is shown here to efficiently methylate its cognate recognition site 5'-GANTC-3' in single-stranded and hemimethylated double-stranded DNA. We report the Km, kcat, kmethylation, and Kd for single-stranded and hemimethylated substrates, revealing discrimination of 10(7)-fold for noncognate sequences. The enzyme also shows a similar discrimination against single-stranded RNA...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28630129/polar-organizing-protein-popz-is-required-for-chromosome-segregation-in-agrobacterium-tumefaciens
#14
Haley M Ehrle, Jacob T Guidry, Rebecca Iacovetto, Anne K Salisbury, D J Sandidge, Grant R Bowman
Despite being perceived as relatively simple organisms, many bacteria exhibit an impressive degree of subcellular organization. In Caulobacter crescentus, the evolutionarily conserved polar organizing protein PopZ facilitates cytoplasmic organization by recruiting chromosome centromeres and regulatory proteins to the cell poles. Here, we characterize the localization and function of PopZ in Agrobacterium tumefaciens, a genetically related species with distinct anatomy. In this species, we find that PopZ molecules are relocated from the old pole to the new pole in the minutes following cell division...
September 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28630123/absence-of-the-polar-organizing-protein-popz-results-in-reduced-and-asymmetric-cell-division-in-agrobacterium-tumefaciens
#15
Matthew Howell, Alena Aliashkevich, Anne K Salisbury, Felipe Cava, Grant R Bowman, Pamela J B Brown
Agrobacterium tumefaciens is a rod-shaped bacterium that grows by polar insertion of new peptidoglycan during cell elongation. As the cell cycle progresses, peptidoglycan synthesis at the pole ceases prior to insertion of new peptidoglycan at midcell to enable cell division. The A. tumefaciens homolog of the Caulobacter crescentus polar organelle development protein PopZ has been identified as a growth pole marker and a candidate polar growth-promoting factor. Here, we characterize the function of PopZ in cell growth and division of A...
September 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28627757/super-resolution-microscopy-and-single-protein-tracking-in-live-bacteria-using-a-genetically-encoded-photostable-fluoromodule
#16
Saumya Saurabh, Adam M Perez, Colin J Comerci, Lucy Shapiro, W E Moerner
Visualization of dynamic protein structures in live cells is crucial for understanding the mechanisms governing biological processes. Fluorescence microscopy is a sensitive tool for this purpose. In order to image proteins in live bacteria using fluorescence microscopy, one typically genetically fuses the protein of interest to a photostable fluorescent tag. Several labeling schemes are available to accomplish this. Particularly, hybrid tags that combine a fluorescent or fluorogenic dye with a genetically encoded protein (such as enzymatic labels) have been used successfully in multiple cell types...
June 19, 2017: Current Protocols in Cell Biology
https://www.readbyqxmd.com/read/28613431/a-conserved-coiled-coil-protein-pair-focuses-the-cytokinetic-z-ring-in-caulobacter-crescentus
#17
Selamawit Abi Woldemeskel, Ryan McQuillen, Alex M Hessel, Jie Xiao, Erin D Goley
The cytoskeletal GTPase FtsZ assembles at midcell, recruits the division machinery and directs envelope invagination for bacterial cytokinesis. ZapA, a conserved FtsZ-binding protein, promotes Z-ring stability and efficient division through a mechanism that is not fully understood. Here, we investigated the function of ZapA in Caulobacter crescentus. We found that ZapA is encoded in an operon with a small coiled-coil protein we named ZauP. ZapA and ZauP co-localized at the division site and were each required for efficient division...
September 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/28598303/caulobacter-rhizosphaerae-sp-nov-a-stalked-bacterium-isolated-from-rhizosphere-soil
#18
Le-Ni Sun, En-Dong Yang, Xue-Ting Hou, Jie-Chao Wei, Zhuo-Xin Yuan, Wei-Yun Wang
The Gram-reaction-negative, aerobic, white- to pale-yellow-coloured and rod-shaped bacterium with a single polar flagellum or a stalk, designated strain 7F14T, was isolated from rhizosphere soil of cultivated watermelon (Citrullus lanatus) collected from Hefei, China. Growth of strain 7F14T was observed at pH 6.0-9.0, 10-30 °C and in the presence of 0-1 % (w/v) NaCl. Cells were catalase-negative and oxidase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 7F14T formed a phyletic lineage within the genus Caulobacter of the family Caulobacteraceae and showed the highest 16S rRNA gene sequence similarities to Caulobacter henricii ATCC 15253T (98...
June 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28593654/linkage-of-microbial-kinetics-and-bacterial-community-structure-of-mbr-and-hybrid-mbbr-mbr-systems-to-treat-salinity-amended-urban-wastewater
#19
Alejandro Rodriguez-Sanchez, Juan Carlos Leyva-Diaz, Alejandro Gonzalez-Martinez, Jose Manuel Poyatos
Three pilot-scale bioreactors were started up and operated under salinity-amended urban wastewater feeding. The bioreactors were configured as membrane bioreactor and two different hybrid, moving bed biofilm reactor-membrane bioreactor and operated with a hydraulic retention time of 9.5 h, a solid residence time of 11.75 days and a total solids concentration of 2500 mg L(-1) . The three systems showed excellent performance in suspended solids, BOD5 , and COD removal (values of 96-100%, 97-99%, and 88-90%, respectively), but poor nitrogen removal (values of 20-30%)...
June 8, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28591613/analysis-of-noise-mechanisms-in-cell-size-control
#20
Saurabh Modi, Cesar Augusto Vargas-Garcia, Khem Raj Ghusinga, Abhyudai Singh
At the single-cell level, noise arises from multiple sources, such as inherent stochasticity of biomolecular processes, random partitioning of resources at division, and fluctuations in cellular growth rates. How these diverse noise mechanisms combine to drive variations in cell size within an isoclonal population is not well understood. Here, we investigate the contributions of different noise sources in well-known paradigms of cell-size control, such as adder (division occurs after adding a fixed size from birth), sizer (division occurs after reaching a size threshold), and timer (division occurs after a fixed time from birth)...
June 6, 2017: Biophysical Journal
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