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https://www.readbyqxmd.com/read/29741143/zinc-finger-nuclease-combines-with-cisplatin-and-trichostatin-a-enhances-the-antitumor-potency-in-cervical-cancer-cells
#1
Ci Ren, Hui Wang, Wencheng Ding, Chun Gao, Xiaomin Li, Hui Shen, Liming Wang, Da Zhu, Peng Wu, Ding Ma
Cervical cancer is the third-most common malignant tumor among women worldwide, which seriously endangers the life and health of Chinese women. The primary etiological factor of cervical cancer is persistent infection with high-risk human papillomavirus (HR-HPV), particularly HPV16 and HPV18. HR-HPV express oncoproteins E6 and E7, both of which play key roles in the progression of cervical carcinogenesis. Zinc Finger Nucleases (ZFNs) targeting HPVE7 induce specific shear of the E7 gene, weakening the malignant biological effects, hence showing great potential for clinical transformation...
May 9, 2018: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/29734518/zinc-finger-nuclease-zfn-mediated-precision-genome-editing-of-an-endogenous-gene-in-hexaploid-bread-wheat-triticum-aestivum-using-a-dna-repair-template
#2
Yidong Ran, Nicola Patron, Pippa Kay, Debbie Wong, Margaret Buchanan, Ying-Ying Cao, Tim Sawbridge, John P Davies, John Mason, Steven R Webb, German Spangenberg, W Michael Ainley, Terence A Walsh, Matthew J Hayden
Sequence-specific nucleases have been used to engineer targeted genome modifications in various plants. While targeted gene knockouts resulting in loss-of-function have been reported with relatively high rates of success, targeted gene editing using an exogenously supplied DNA repair template and site-specific transgene integration has been more challenging. Here, we report the first application of Zinc Finger Nuclease (ZFN)-mediated, non-homologous end joining (NHEJ)-directed editing of a native gene in allohexaploid bread wheat to introduce, via a supplied DNA repair template, a specific single amino acid change into the coding sequence of acetohydroxyacid synthase (AHAS) to confer resistance to imidazolinone herbicides...
May 7, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29657233/reproductive-technologies-for-the-generation-and-maintenance-of-valuable-animal-strains
#3
Takehito Kaneko
Many types of mutant and genetically engineered strains have been produced in various animal species. Their numbers have dramatically increased in recent years, with new strains being rapidly produced using genome editing techniques. In the rat, it has been difficult to produce knockout and knock-in strains because the establishment of stem cells has been insufficient. However, a large number of knockout and knock-in strains can currently be produced using genome editing techniques, including zinc-finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) system...
April 14, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29652634/emerging-applications-of-genome-editing-technology-to-examine-functionality-of-gwas-associated-variants-for-complex-traits
#4
Andrew J P Smith, Panos Deloukas, Patricia B Munroe
Over the last decade, genome-wide association studies (GWAS) have propelled the discovery of thousands of loci associated with complex diseases. The focus is now turning towards the function of these association signals, determining the causal variant(s) amongst those in strong linkage disequilibrium, and identifying their underlying mechanisms, such as long-range gene regulation. Genome-editing techniques utilising zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALENs) and clustered regularly-interspaced short palindromic repeats with Cas9 nuclease (CRISPR-Cas9), are becoming the tools of choice to establish functionality for these variants, due to the ability to assess effects of single variants in vivo...
April 13, 2018: Physiological Genomics
https://www.readbyqxmd.com/read/29617316/study-of-photochemical-cytosine-to-uracil-transition-via-ultrafast-photo-cross-linking-using-vinylcarbazole-derivatives-in-duplex-dna
#5
Siddhant Sethi, Shigetaka Nakamura, Kenzo Fujimoto
Gene therapies, including genome editing, RNAi, anti-sense technology and chemical DNA editing are becoming major methods for the treatment of genetic disorders. Techniques like CRISPR-Cas9, zinc finger nuclease (ZFN) and transcription activator-like effector-based nuclease (TALEN) are a few such enzymatic techniques. Most enzymatic genome editing techniques have their disadvantages. Thus, non-enzymatic and non-invasive technologies for nucleic acid editing has been reported in this study which might possess some advantages over the older methods of DNA manipulation...
April 4, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29589874/genome-editing-in-neuroepithelial-stem-cells-to-generate-human-neurons-with-high-adenosine-releasing-capacity
#6
Daniel Poppe, Jonas Doerr, Marion Schneider, Ruven Wilkens, Julius A Steinbeck, Julia Ladewig, Allison Tam, David E Paschon, Philip D Gregory, Andreas Reik, Christa E Müller, Philipp Koch, Oliver Brüstle
As a powerful regulator of cellular homeostasis and metabolism, adenosine is involved in diverse neurological processes including pain, cognition, and memory. Altered adenosine homeostasis has also been associated with several diseases such as depression, schizophrenia, or epilepsy. Based on its protective properties, adenosine has been considered as a potential therapeutic agent for various brain disorders. Since systemic application of adenosine is hampered by serious side effects such as vasodilatation and cardiac suppression, recent studies aim at improving local delivery by depots, pumps, or cell-based applications...
March 28, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29580682/dose-dependent-prevention-of-metabolic-and-neurologic-disease-in-murine-mps-ii-by-zfn-mediated-in-vivo-genome-editing
#7
Kanut Laoharawee, Russell C DeKelver, Kelly M Podetz-Pedersen, Michelle Rohde, Scott Sproul, Hoang-Oanh Nguyen, Tam Nguyen, Susan J St Martin, Li Ou, Susan Tom, Robert Radeke, Kathleen E Meyer, Michael C Holmes, Chester B Whitley, Thomas Wechsler, R Scott McIvor
Mucopolysaccharidosis type II (MPS II) is an X-linked recessive lysosomal disorder caused by deficiency of iduronate 2-sulfatase (IDS), leading to accumulation of glycosaminoglycans (GAGs) in tissues of affected individuals, progressive disease, and shortened lifespan. Currently available enzyme replacement therapy (ERT) requires lifelong infusions and does not provide neurologic benefit. We utilized a zinc finger nuclease (ZFN)-targeting system to mediate genome editing for insertion of the human IDS (hIDS) coding sequence into a "safe harbor" site, intron 1 of the albumin locus in hepatocytes of an MPS II mouse model...
March 10, 2018: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29575905/efficient-zfn-mediated-stop-codon-integration-into-the-ccr5-locus-in-hematopoietic-stem-cells-a-possible-source-for-intra-bone-marrow-cell-transplantation
#8
Supreecha Chattong, Kamontip Chaikomon, Theerasak Chaiya, Thitirat Tangkosakul, Nattawan Palavutitotai, Thitinan Anusornvongchai, Krissanapong Manotham
We reported a simple genome editing approach that can generate HIV co-receptor defective cells, which may be useful for latent viral eradication treatment. Samples of bone marrow left over after diagnostic procedures and crude bone marrow from aviremic HIV patients were subjected to zinc finger nuclease (ZFN)-mediated stop codon insertion into CCR5 loci. Locked nucleic acid (LNA)-based PCR was employed to estimate the amount of insertion in the expandable CD34+ cells. The results showed that about 0.5% of CD34+ cells carried stop codon insertions in CCR5 loci...
March 25, 2018: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29570433/targeted-disruption-of-regulated-endocrine-specific-protein-resp18-in-dahl-ss-mcw-rats-aggravates-salt-induced-hypertension-and-renal-injury
#9
Sivarajan Kumarasamy, Harshal Waghulde, Xi Cheng, Steven T Haller, Blair Mell, Basrur Abhijith, Usman M Ashraf, Ealla Atari, Bina Joe
Hypertension is a classic example of a complex polygenic trait, impacted by quantitative trait loci (QTL) containing candidate genes thought to be responsible for blood pressure (BP) control in mammals. One such mapped locus is on rat chromosome 9, wherein the proof for a positional candidate gene, Regulated Endocrine Specific Protein-18 (Resp18) is currently inadequate. To ascertain the status of Resp18 as a BP QTL, a custom targeted gene disruption model of Resp18 was developed on the Dahl Salt-Sensitive (SS) background...
March 23, 2018: Physiological Genomics
https://www.readbyqxmd.com/read/29555822/programmable-single-and-multiplex-base-editing-in-bombyx-mori-using-rna-guided-cytidine-deaminases
#10
Yufeng Li, Sanyuan Ma, Le Sun, Tong Zhang, Jiasong Chang, Wei Lu, Xiaoxu Chen, Yue Liu, Xiaogang Wang, Run Shi, Ping Zhao, Qingyou Xia
Genome editing using standard tools (ZFN, TALEN, and CRISPR/Cas9) rely on double strand breaks to edit the genome. A series of new CRISPR tools that convert cytidine to thymine (C to T) without the requirement for DNA double-strand breaks was developed recently and quickly applied in a variety of organisms. Here, we demonstrate that CRISPR/Cas9-dependent base editor (BE3) converts C to T with a high frequency in the invertebrate Bombyx mori silkworm. Using BE3 as a knock-out tool, we inactivated exogenous and endogenous genes through base-editing-induced nonsense mutations with an efficiency of up to 66...
March 19, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29523384/application-and-development-of-genome-editing-technologies-to-the-solanaceae-plants
#11
REVIEW
Tsuyoshi Yamamoto, Sachiko Kashojiya, Saori Kamimura, Takato Kameyama, Tohru Ariizumi, Hiroshi Ezura, Kenji Miura
Genome editing technology using artificial nucleases, including zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), and clustered regulatory interspaced short palindromic repeats (CRISPR)-Cas9, can mutagenize the target sites of genes of interest. This technology has been successfully applied in several crops, including the Solanaceae plants, such as tomato, potato, tobacco, and petunia. Among the three nucleases, CRISPR-Cas9 is the best for breeding, crop improvement, and the functional analysis of genes of interest, because of its simplicity and high efficiency...
March 2, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29423215/development-of-genome-engineering-technologies-in-cattle-from-random-to-specific
#12
REVIEW
Soo-Young Yum, Ki-Young Youn, Woo-Jae Choi, Goo Jang
The production of transgenic farm animals (e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos (zygotes) followed by embryo transfer into a recipient was performed because of the ease of the procedure. However, as this approach resulted in severe mosaicism and has a low efficiency, it is not typically employed in the cattle as priority, unlike in mice. To overcome the above issue with micro-injection in cattle, somatic cell nuclear transfer (SCNT) was introduced and successfully used to produce cloned livestock...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29348190/pronounced-hyperactivity-cognitive-dysfunctions-and-bdnf-dysregulation-in-dopamine-transporter-knock-out-rats
#13
Damiana Leo, Ilya Sukhanov, Francesca Zoratto, Placido Illiano, Lucia Caffino, Fabrizio Sanna, Giulia Messa, Marco Emanuele, Alessandro Esposito, Mariia Dorofeikova, Evgeny A Budygin, Liudmila Mus, Evgenia V Efimova, Marco Niello, Stefano Espinoza, Tatyana D Sotnikova, Marius C Hoener, Giovanni Laviola, Fabio Fumagalli, Walter Adriani, Raul R Gainetdinov
Dopamine (DA) controls many vital physiological functions and is critically involved in several neuropsychiatric disorders such as schizophrenia and attention deficit hyperactivity disorder. The major function of the plasma membrane dopamine transporter (DAT) is the rapid uptake of released DA into presynaptic nerve terminals leading to control of both the extracellular levels of DA and the intracellular stores of DA. Here, we present a newly developed strain of rats in which the gene encoding DAT knockout Rats (DAT-KO) has been disrupted by using zinc finger nuclease technology...
February 21, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29301855/gene-editing-as-a-promising-approach-for-respiratory-diseases
#14
Yichun Bai, Yang Liu, Zhenlei Su, Yana Ma, Chonghua Ren, Runzhen Zhao, Hong-Long Ji
Respiratory diseases, which are leading causes of mortality and morbidity in the world, are dysfunctions of the nasopharynx, the trachea, the bronchus, the lung and the pleural cavity. Symptoms of chronic respiratory diseases, such as cough, sneezing and difficulty breathing, may seriously affect the productivity, sleep quality and physical and mental well-being of patients, and patients with acute respiratory diseases may have difficulty breathing, anoxia and even life-threatening respiratory failure. Respiratory diseases are generally heterogeneous, with multifaceted causes including smoking, ageing, air pollution, infection and gene mutations...
March 2018: Journal of Medical Genetics
https://www.readbyqxmd.com/read/29285485/a-novel-prokaryotic-green-fluorescent-protein-expression-system-for-testing-gene-editing-tools-activity-like-zinc-finger-nuclease
#15
Faezeh Sabzehei, Shirin Kouhpayeh, Mansoureh Shahbazi Dastjerdeh, Hossein Khanahmad, Rasoul Salehi, Shamsi Naderi, Razieh Taghizadeh, Parisa Rabiei, Zahra Hejazi, Laleh Shariati
Background: Gene editing technology has created a revolution in the field of genome editing. The three of the most famous tools in gene editing technology are zinc finger nucleases (ZFNs), transcription activator-like effector nucleases, clustered regularly interspaced short palindromic repeats (CRISPR), and CRISPR-associated systems. As their predictable nature, it is necessary to assess their efficiency. There are some methods for this purpose, but most of them are time labor and complicated...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29196142/new-transgenic-models-of-parkinson-s-disease-using-genome-editing-technology
#16
J A Cota-Coronado, S Sandoval-Ávila, Y P Gaytan-Dávila, N F Diaz, B Vega-Ruiz, E Padilla-Camberos, N E Díaz-Martínez
INTRODUCTION: Parkinson's disease (PD) is the second most common neurodegenerative disorder. It is characterised by selective loss of dopaminergic neurons in the substantia nigra pars compacta, which results in dopamine depletion, leading to a number of motor and non-motor symptoms. DEVELOPMENT: In recent years, the development of new animal models using nuclease-based genome-editing technology (ZFN, TALEN, and CRISPR/Cas9 nucleases) has enabled the introduction of custom-made modifications into the genome to replicate key features of PD, leading to significant advances in our understanding of the pathophysiology of the disease...
November 28, 2017: Neurología: Publicación Oficial de la Sociedad Española de Neurología
https://www.readbyqxmd.com/read/29183134/in-vitro-transduction-and-target-mutagenesis-efficiency-of-hiv-1-pol-gene-targeting-zfn-and-crispr-cas9-delivered-by-various-plasmids-and-or-vectors-toward-an-hiv-cure
#17
Moses Okee, Alice Bayiyana, Carol Musubika, Moses L Joloba, Fred Ashaba-Katabazi, Bernard Bagaya, Misaki Wayengera
Efficiency of artificial restriction enzymes toward curing HIV has only been separately examined, using differing delivery vehicles. We compared the in vitro transduction and target-mutagenesis efficiency of consortium plasmid and adenoviral vector delivered HIV-1 pol gene targeting zinc finger nuclease (ZFN) with CRISPR/Cas, Custom-ZFN, CRISPR-Cas-9, and plasmids and vectors (murCTSD_pZFN, pGS-U-gRNA, pCMV-Cas-D01A, Ad5-RGD); cell lines (TZM-bl and ACH-2/J-Lat cells); and the latency reversing agents prostratin, suberoylanilide hydroxamic acid, and phorbol myristate acetate...
January 2018: AIDS Research and Human Retroviruses
https://www.readbyqxmd.com/read/29163669/current-status-and-perspectives-of-genome-editing-technology-for-microalgae
#18
REVIEW
Seungjib Jeon, Jong-Min Lim, Hyung-Gwan Lee, Sung-Eun Shin, Nam Kyu Kang, Youn-Il Park, Hee-Mock Oh, Won-Joong Jeong, Byeong-Ryool Jeong, Yong Keun Chang
Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been greatly facilitated by engineered nucleases, dubbed molecular scissors, including zinc-finger nuclease (ZFN), TAL effector endonuclease (TALEN) and clustered regularly interspaced palindromic sequences (CRISPR)/Cas9. In particular, CRISPR/Cas9 has revolutionized genome editing fields with its simplicity, efficiency and accuracy compared to previous nucleases...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29150002/gene-editing-and-crispr-therapeutics-strategies-taught-by-cell-and-gene-therapy
#19
Juan C Ramirez
A few years ago, we assisted in the demonstration for the first time of the revolutionary idea of a type of adaptive-immune system in the bacteria kingdom. This system, named CRISPR, and variants engineered in the lab, have been demonstrated as functional with extremely high frequency and fidelity in almost all eukaryotic cells studied to date. The capabilities of this RNA-guided nuclease have added to the interest that was announced with the advent of previous technologies for genome editing tools, such as ZFN and TALEN...
2017: Progress in Molecular Biology and Translational Science
https://www.readbyqxmd.com/read/29099045/gene-editing-in-human-lymphoid-cells-role-for-donor-dna-type-of-genomic-nuclease-and-cell-selection-method
#20
Anastasia Zotova, Elena Lopatukhina, Alexander Filatov, Musa Khaitov, Dmitriy Mazurov
Programmable endonucleases introduce DNA breaks at specific sites, which are repaired by non-homologous end joining (NHEJ) or homology recombination (HDR). Genome editing in human lymphoid cells is challenging as these difficult-to-transfect cells may also inefficiently repair DNA by HDR. Here, we estimated efficiencies and dynamics of knockout (KO) and knockin (KI) generation in human T and B cell lines depending on repair template, target loci and types of genomic endonucleases. Using zinc finger nuclease (ZFN), we have engineered Jurkat and CEM cells with the 8...
November 2, 2017: Viruses
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