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https://www.readbyqxmd.com/read/28921495/probing-rna-folding-pathways-by-rna-fingerprinting
#1
Sarah A Woodson
This unit provides protocols for using native polyacrylamide gel electrophoresis to distinguish folding and unfolding conformers of RNA. It is useful for studying conformers that can exchange in a period of minutes or seconds, and that are thus difficult to study by solution-based methods. Conformers that have been separated and immobilized in the gel matrix can be used to study catalytic activity with or without being eluted from the gel. The method can be applied to a wide variety of catalytic RNAs and RNA-protein complexes...
September 18, 2017: Current Protocols in Nucleic Acid Chemistry
https://www.readbyqxmd.com/read/28887451/evolution-of-protein-coupled-rna-dynamics-during-hierarchical-assembly-of-ribosomal-complexes
#2
Sanjaya C Abeysirigunawardena, Hajin Kim, Jonathan Lai, Kaushik Ragunathan, Mollie C Rappé, Zaida Luthey-Schulten, Taekjip Ha, Sarah A Woodson
Assembly of 30S ribosomes involves the hierarchical addition of ribosomal proteins that progressively stabilize the folded 16S rRNA. Here, we use three-color single molecule FRET to show how combinations of ribosomal proteins uS4, uS17 and bS20 in the 16S 5' domain enable the recruitment of protein bS16, the next protein to join the complex. Analysis of real-time bS16 binding events shows that bS16 binds both native and non-native forms of the rRNA. The native rRNA conformation is increasingly favored after bS16 binds, explaining how bS16 drives later steps of 30S assembly...
September 8, 2017: Nature Communications
https://www.readbyqxmd.com/read/28844793/utilization-and-outcomes-of-radical-cystectomy-for-high-grade-non-muscle-invasive-bladder-cancer-in-elderly-patients
#3
William P Parker, Woodson Smelser, Eugene K Lee, Elizabeth B Habermann, Prabin Thapa, Harras B Zaid, Igor Frank, Tomas L Griebling, Matthew K Tollefson, R Houston Thompson, Jeffrey M Holzbeierlein, R Jeffrey Karnes, Stephen A Boorjian
BACKGROUND: Radical cystectomy (RC) represents a treatment option for patients with high-grade non-muscle-invasive bladder cancer (HG-NMIBC); however, perioperative morbidity is not insignificant, particularly in elderly patients. We sought to evaluate the associations of age with utilization and outcomes of RC for HG-NMIBC. PATIENTS AND METHODS: Patients with HG-NMIBC diagnosed between 2004 and 2013 were identified in the National Cancer Database and stratified by age: ≤ 60, 61-70, 71-80, and > 80 years...
August 1, 2017: Clinical Genitourinary Cancer
https://www.readbyqxmd.com/read/28826489/acidic-c-terminal-domains-autoregulate-the-rna-chaperone-hfq
#4
Andrew Santiago-Frangos, Jeliazko R Jeliazkov, Jeffrey J Gray, Sarah A Woodson
The RNA chaperone Hfq is an Sm protein that facilitates base pairing between bacterial small RNAs (sRNAs) and mRNAs involved in stress response and pathogenesis. Hfq possesses an intrinsically disordered C-terminal domain (CTD) that may tune the function of the Sm domain in different organisms. In Escherichia coli, the Hfq CTD increases kinetic competition between sRNAs and recycles Hfq from the sRNA-mRNA duplex. Here, de novo Rosetta modeling and competitive binding experiments show that the acidic tip of the E...
August 9, 2017: ELife
https://www.readbyqxmd.com/read/28825710/metals-induce-transient-folding-and-activation-of-the-twister-ribozyme
#5
Subrata Panja, Boyang Hua, Diego Zegarra, Taekjip Ha, Sarah A Woodson
Twister is a small ribozyme present in almost all kingdoms of life that rapidly self-cleaves in variety of divalent metal ions. We used activity assays, bulk FRET and single-molecule FRET (smFRET) to understand how different metal ions promote folding and self-cleavage of the Oryza sativa twister ribozyme. Although most ribozymes require additional Mg(2+) for catalysis, twister inverts this expectation, requiring 20-30 times less Mg(2+) to self-cleave than to fold. Transition metals such as Co(2+), Ni(2+) and Zn(2+) activate twister more efficiently than Mg(2+) ions...
October 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28810953/continuous-leadership-education-and-development-at-the-san-antonio-uniformed-services-health-education-consortium
#6
Sarah N Bowe, Woodson Scott Jones
No abstract text is available yet for this article.
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28796571/the-fate-and-impact-of-internal-waves-in-nearshore-ecosystems
#7
C B Woodson
Internal waves are widespread features of global oceans that play critical roles in mixing and thermohaline circulation. Similarly to surface waves, internal waves can travel long distances, ultimately breaking along continental margins. These breaking waves can transport deep ocean water and associated constituents (nutrients, larvae, and acidic low-oxygen waters) onto the shelf and locally enhance turbulence and mixing, with important effects on nearshore ecosystems. We are only beginning to understand the role internal waves play in shaping nearshore ecosystems...
August 10, 2017: Annual Review of Marine Science
https://www.readbyqxmd.com/read/28738333/updated-hypopharyngeal-surgery-for-sleep-apnea
#8
B Tucker Woodson
The failure of traditional upper airway surgery such as uvulopalatopharyngoplasty has been attributed to lower airway obstruction related to base of tongue collapse. Multiple procedures, including glossectomy, tongue base radiofrequency, genioglossus advancement, and tongue suspension techniques, have been advocated to improve success rates. No consensus exists on which subsets of patients are best treated by individual approaches and direct comparative data are lacking. The selection of procedures must be based on individual patient needs and the relative potential benefits and risks...
2017: Advances in Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28688564/utility-of-intraoperative-and-postoperative-radiographs-in-pediatric-cochlear-implant-surgery
#9
Samantha Anne, Jose Miguel Juarez, Amber Shaffer, David Eleff, Dennis Kitsko, Sarah Sydlowski, Erika Woodson, David Chi
OBJECTIVE: Routine plain film radiographs are often obtained to confirm proper placement of electrode after pediatric cochlear implant surgery. Objective is to evaluate necessity of routine radiographs in pediatric cochlear implant cases. STUDY DESIGN: Retrospective review. SETTING: Two tertiary care academic centers. SUBJECTS AND METHODS: Review of all children that underwent cochlear implantation from January 2003 thru June 2015...
August 2017: International Journal of Pediatric Otorhinolaryngology
https://www.readbyqxmd.com/read/28683262/untangling-the-neural-circuits-for-sexual-behavior
#10
Jonathan Woodson, Addison Niemeyer, Joseph Bergan
In this issue, Ishii and colleagues identify an anatomically and genetically defined circuit by which a single compound, exocrine gland-secreted peptide-1 (ESP1), enhances reproductive behavior in female mice.
July 5, 2017: Neuron
https://www.readbyqxmd.com/read/28681961/technical-tips-during-implantation-of-selective-upper-airway-stimulation
#11
Clemens Heiser, Erica Thaler, Ryan J Soose, B Tucker Woodson, Maurits Boon
OBJECTIVE: Selective upper airway stimulation is now well-established in the United States and in several European countries, with more than 1,000 patients implanted since U.S. Food and Drug Administration approval in April 2014. The authors herein, all head and neck surgeons, account for approximately one of every five implants completed to date. Several of the authors also provide comprehensive longitudinal care of their patients as dual-specialty sleep medicine physicians. STUDY DESIGN: Multi-center, retrospective clinical analysis...
July 6, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28632384/finite-field-method-for-nonlinear-optical-property-prediction-using-rational-function-approximants
#12
Anand H G Patel, Ahmed A K Mohammed, Peter A Limacher, Paul W Ayers
The finite field (FF) method is a quick, easy-to-implement tool for the prediction of nonlinear optical properties. Here, we present and explore a novel variant of the FF method, which uses a rational function to fit a molecule's energy with respect to an electric field. Similarly to previous FF methods, factors crucial for the method's accuracy were tuned. These factors include the number of terms in the function, the distribution of fields used to construct the approximation, and the initial field in the approximation...
July 6, 2017: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/28632078/-less-than-a-vapor-positioning-black-lesbian-women-in-history-teacher-education
#13
Ashley N Woodson
In this article, I discuss the possibilities and implications of centering Black lesbian identities and relationships in history teacher education through a case study with one straight Black woman preservice history teacher named Danitra. Danitra's understanding and navigation of historical research on Black lesbians are discussed in relation to core themes of lesbian historiography and emancipatory historiography. Though the literature on this group is limited, I argue that critical considerations of Black lesbians' interests and experiences help educators to conceive of and teach about history, citizenship, justice, and sexuality in more liberatory ways...
September 22, 2016: Journal of Lesbian Studies
https://www.readbyqxmd.com/read/28596260/evaluating-the-nccn-clinical-criteria-for-recommending-brca1-and-brca2-genetic-testing-in-patients-with-breast-cancer
#14
Caiqian Cropper, Ashley Woodson, Banu Arun, Carlos Barcenas, Jennifer Litton, Sarah Noblin, Diane Liu, Minjeong Park, Molly Daniels
Background: Mutations in the BRCA1 and BRCA2 genes predispose individuals to a significantly elevated risk for breast and ovarian cancers. Identification of these individuals allows for proper screening, management, and testing of at-risk relatives. NCCN has established clinical criteria for recommending BRCA1/2 testing. Patients and Methods: A retrospective chart review of 1,123 patients with breast cancer was performed to evaluate the positive predictive values (PPVs) of 14 individual criteria for predicting BRCA1/2 mutations...
June 2017: Journal of the National Comprehensive Cancer Network: JNCCN
https://www.readbyqxmd.com/read/28595273/acupuncture-therapy-in-a-group-setting-for-chronic-pain
#15
Benjamin Kligler, Arya Nielsen, Corinne Kohrrer, Tracy Schmid, Eve Waltermaurer, Elidania Perez, Woodson Merrell
Objective.:  This project was designed to test the feasibility and effectiveness of acupuncture therapy given in a group setting for chronic pain. Design.:  Nonrandomized, repeated measures quasi-experimental trial. Setting.:  Care was delivered in a primary care clinic waiting area after clinic hours. Subjects.:  Included were primary care patients (≥18 years old) with chronic pain of the neck, back, shoulder, or osteoarthritis of any site of at least three months' duration...
June 8, 2017: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/28578544/effects-of-minimal-versus-intensive-intervention-to-enhance-motivational-interviewing-in-hiv-care
#16
Mary Catherine Beach, M Barton Laws, Gary Rose, Debra Roter, Yoojin Lee, Geetanjali Chander, Tanita Woodson, Richard D Moore, William Rogers, Ira B Wilson
We conducted a randomized trial comparing the effect of two different levels of motivational interviewing training on clinician communication behaviors and patient experiences. We enrolled 12 HIV clinicians who attended a one-day MI workshop focusing on behavior change counseling skills. We then randomized clinicians to receive (or not) 3-5 rounds of personalized feedback from the MI trainer. We compared outcomes before and after the interventions and between the intervention groups. We tested time-by-study arm interactions to determine if one group improved more than the other...
June 3, 2017: AIDS and Behavior
https://www.readbyqxmd.com/read/28562608/correction-active-zone-proteins-are-transported-via-distinct-mechanisms-regulated-by-par-1-kinase
#17
Kara R Barber, Julia Tanquary, Keegan Bush, Amanda Shaw, Michael Woodson, Karthikeyan Tangavelou, Michael Sherman, Yogesh P Wairkar
[This corrects the article DOI: 10.1371/journal.pgen.1006621.].
May 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28522763/aortic-arch-compliance-and-idiopathic-unilateral-vocal-fold-paralysis
#18
Reza Behkam, Kara E Roberts, Andrew J Bierhals, M Eileen Jacobs, Julia D Edgar, Randal C Paniello, Gayle Woodson, Jonathan P Vande Geest, Julie M Barkmeier-Kraemer
Unilateral vocal fold paralysis (UVP) occurs related to recurrent laryngeal nerve (RLN) impairment associated with impaired swallowing, voice production, and breathing functions. The majority of UVP cases occur subsequent to surgical intervention with approximately 12-42% having no known cause for the disease (i.e., idiopathic). Approximately 2/3 of those with UVP exhibit left-sided injury with the average onset at 50 years of age or older in those diagnosed as idiopathic. Given the association between the RLN and the subclavian and aortic arch vessels, we hypothesized that changes in vascular tissues would result in increased aortic compliance in idiopathic left-sided UVP patients compared to those without UVP...
May 18, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28469298/where-are-we-with-bladder-preservation-for-muscle-invasive-bladder-cancer-in-2017
#19
REVIEW
Woodson Wade Smelser, Marcus A Austenfeld, Jeffrey Maxwell Holzbeierlein, Eugene Kang Lee
INTRODUCTION: In 2017, neoadjuvant, cisplatin-based chemotherapy followed by radical cystectomy (RC) is considered the gold standard therapy for muscle-invasive bladder based on randomized controlled trials. Across all tumor stages, this approach has been associated with the highest rates of disease-specific survival. However, RC is one of the most challenging procedures performed by urologic surgeons and carries with it significant risks of complications, hospital readmission, and even a small risk of mortality, in addition to lifestyle changes that can have long-term effects on well-being...
April 2017: Indian Journal of Urology: IJU: Journal of the Urological Society of India
https://www.readbyqxmd.com/read/28442428/rapamycin-modulation-of-p70-s6-kinase-signaling-inhibits-rift-valley-fever-virus-pathogenesis
#20
Todd M Bell, Virginia Espina, Svetlana Senina, Caitlin Woodson, Ashwini Brahms, Brian Carey, Shih-Chao Lin, Lindsay Lundberg, Chelsea Pinkham, Alan Baer, Claudius Mueller, Elizabeth A Chlipala, Faye Sharman, Cynthia de la Fuente, Lance Liotta, Kylene Kehn-Hall
Despite over 60 years of research on antiviral drugs, very few are FDA approved to treat acute viral infections. Rift Valley fever virus (RVFV), an arthropod borne virus that causes hemorrhagic fever in severe cases, currently lacks effective treatments. Existing as obligate intracellular parasites, viruses have evolved to manipulate host cell signaling pathways to meet their replication needs. Specifically, translation modulation is often necessary for viruses to establish infection in their host. Here we demonstrated phosphorylation of p70 S6 kinase, S6 ribosomal protein, and eIF4G following RVFV infection in vitro through western blot analysis and in a mouse model of infection through reverse phase protein microarrays (RPPA)...
July 2017: Antiviral Research
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