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https://www.readbyqxmd.com/read/29475025/protein-anchoring-therapy-to-target-extracellular-matrix-proteins-to-their-physiological-destinations
#1
REVIEW
Mikako Ito, Kinji Ohno
Endplate acetylcholinesterase (AChE) deficiency is a form of congenital myasthenic syndrome (CMS) caused by mutations in COLQ, which encodes collagen Q (ColQ). ColQ is an extracellular matrix (ECM) protein that anchors AChE to the synaptic basal lamina. Biglycan, encoded by BGN, is another ECM protein that binds to the dystrophin-associated protein complex (DAPC) on skeletal muscle, which links the actin cytoskeleton and ECM proteins to stabilize the sarcolemma during repeated muscle contractions. Upregulation of biglycan stabilizes the DPAC...
February 20, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29474833/one-step-integrated-clarification-and-purification-of-a-monoclonal-antibody-using-protein-a-mag-sepharose-beads-and-a-cgmp-compliant-high-gradient-magnetic-separator
#2
Moritz Ebeler, Ola Lind, Nils Norrman, Ronnie Palmgren, Matthias Franzreb
Monoclonal antibodies are a dominant component of today's biopharmaceutical market and are typically purified by classical platform processes. However, high costs and rising demands are drivers for the development of new, efficient and flexible integrated purification processes. Currently, high-gradient magnetic separation as a direct capturing tool for protein purification suffers from the lack of suitable GMP-compliant separation equipment for industrial scale. As a solution for this bottleneck, we present a purification process for a monoclonal antibody directly from CHO cell culture by use of protein A-functionalized magnetic particles together with the first pilot-scale GMP-compliant 'rotor-stator' high-gradient magnetic separator...
February 20, 2018: New Biotechnology
https://www.readbyqxmd.com/read/29474381/comprehensive-analysis-of-clinical-burkholderia-pseudomallei-isolates-demonstrates-conservation-of-unique-lipid-a-structure-and-tlr4-dependent-innate-immune-activation
#3
Sineenart Sengyee, Sung Hwan Yoon, Suporn Paksanont, Thatcha Yimthin, Vanaporn Wuthiekanun, Direk Limmathurotsakul, T Eoin West, Robert K Ernst, Narisara Chantratita
Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with a range of clinical symptoms, is often characterized by a robust inflammatory response, may relapse after treatment, and results in high mortality rates. Lipopolysaccharide (LPS) of B. pseudomallei is a potent immunostimulatory molecule comprised of lipid A, core, and O-polysaccharide (OPS) components. Four B. pseudomallei LPS types have been described based on SDS-PAGE patterns that represent the difference of OPS-type A, type B, type B2 and rough LPS...
February 23, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29474374/neither-lys-and-dap-type-peptidoglycans-stimulate-mouse-or-human-innate-immune-cells-via-toll-like-receptor-2
#4
Marybeth Langer, Alanson W Girton, Narcis I Popescu, Tarea Burgett, Jordan P Metcalf, K Mark Coggeshall
Peptidoglycan (PGN), a major component of bacterial cell walls, is a pathogen-associated molecular pattern (PAMP) that causes innate immune cells to produce inflammatory cytokines that escalate the host response during infection. In order to better understand the role of PGN in infection, we wanted to gain insight into the cellular receptor for PGN. Although the receptor was initially identified as Toll-like receptor 2 (TLR2), this receptor has remained controversial and other PGN receptors have been reported...
2018: PloS One
https://www.readbyqxmd.com/read/29473346/evaluating-interaction-of-cord-blood-hematopoietic-stem-progenitor-cells-with-functionally-integrated-three-dimensional-microenvironments
#5
Saloomeh Mokhtari, Pedro M Baptista, Dipen A Vyas, Charles Jordan Freeman, Emma Moran, Matthew Brovold, Guillermo A Llamazares, Zanneta Lamar, Christopher D Porada, Shay Soker, Graça Almeida-Porada
Despite advances in ex vivo expansion of cord blood-derived hematopoietic stem/progenitor cells (CB-HSPC), challenges still remain regarding the ability to obtain, from a single unit, sufficient numbers of cells to treat an adolescent or adult patient. We and others have shown that CB-HSPC can be expanded ex vivo in two-dimensional (2D) cultures, but the absolute percentage of the more primitive stem cells decreases with time. During development, the fetal liver is the main site of HSPC expansion. Therefore, here we investigated, in vitro, the outcome of interactions of primitive HSPC with surrogate fetal liver environments...
February 23, 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29471524/enteric-methane-production-and-ruminal-fermentation-of-forage-brassica-diets-fed-in-continuous-culture
#6
S Leanne Dillard, Ana I Roca-Fernández, Melissa D Rubano, Kyle R Elkin, Kathy J Soder
The aim of the current study was to determine nutrient digestibility, VFA production, N metabolism, and CH4 production of canola (Brassica napus L.), rapeseed (B. napus L.), turnip (B. rapa L.), and annual ryegrass (Lolium multiflorum Lam.) fed with orchardgrass (Dactylis glomerata L.) in continuous culture. Diets were randomly assigned to fermentors in a 4 × 4 Latin square design using 7 d for adaptation and 3 d for collection. Diets were: 1) 50% orchardgrass + 50% annual ryegrass (ARG); 2) 50% orchardgrass + 50% canola (CAN); 3) 50% orchardgrass + 50% rapeseed (RAP); and 4) 50% orchardgrass + 50% turnip (TUR)...
February 17, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29471498/-it-was-the-best-worst-day-of-my-life-narrative-content-structure-and-process-in-wisdom-fostering-life-event-memories
#7
Nic M Weststrate, Michel Ferrari, Marc A Fournier, Kate C McLean
Objectives: The purpose of this study was to examine laypeople's subjective understanding of their own wisdom development. To do this, autobiographical memories of wisdom-fostering life events were examined for (a) life-event characteristics, and (b) self-reflective processes believed to support growth in wisdom through life experience. Methods: Midlife adults (N = 482) provided a written autobiographical memory of a wisdom-fostering life event. Memories were content analyzed by expert coders for life-event characteristics (i...
February 20, 2018: Journals of Gerontology. Series B, Psychological Sciences and Social Sciences
https://www.readbyqxmd.com/read/29471278/intestinal-bacteria-in-bioaerosols-and-factors-affecting-their-survival-in-two-oxidation-ditch-process-municipal-wastewater-treatment-plants-located-in-different-regions
#8
Yanjie Wang, Lin Li, Yunping Han, Junxin Liu, Kaixiong Yang
Samples from two oxidation ditch process municipal wastewater treatment plants (MWTPs) (HJK and GXQ) in two regions of China were analysed for bacteria, particles, total organic carbon, and water-soluble ions in bioaerosols. Diversity and potential pathogen populations were evaluated by high-throughput sequencing. Bioaerosol sources, factors affecting intestinal bacterial survival, and the relationship between bioaerosols and water were analysed by Source tracker and partial least squares-discriminant, principal component, and canonical correspondence analyses...
February 19, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29470747/solid-stress-facilitates-fibroblasts-activation-to-promote-pancreatic-cancer-cell-migration
#9
Maria Kalli, Panagiotis Papageorgis, Vasiliki Gkretsi, Triantafyllos Stylianopoulos
Pancreatic fibroblasts are continuously gaining ground as an important component of tumor microenvironment that dynamically interact with cancer cells to promote tumor progression. In addition, these tumor-infiltrated fibroblasts can acquire an activated phenotype and produce excessive amounts of extracellular matrix creating a highly dense stroma, a situation known as desmoplasia. Desmoplasia, along with the uncontrolled proliferation of cancer cells, leads to the development of compressive forces within the tumor, generating the so-called solid stress...
February 22, 2018: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/29470477/m%C3%A4-ori-indigenous-health-framework-in-action-addressing-ethnic-disparities-in-healthcare
#10
Ibrahim S Al-Busaidi, Tania Huria, Suzanne Pitama, Cameron Lacey
There has been a steady increase in cultural competency training in medical education programmes worldwide. To provide high-quality culturally competent care and reduce health disparities between Māori and non-Māori in New Zealand, several health models have been devised. The Indigenous Health Framework (IHF), currently taught at the University of Otago, Christchurch undergraduate medical programme, is a tool developed to assist health professionals to broaden their range of clinical assessment and communicate effectively with Māori patients and whānau, thereby improving health outcomes and reducing disparities...
February 23, 2018: New Zealand Medical Journal
https://www.readbyqxmd.com/read/29468596/an-in-vitro-oxygen-glucose-deprivation-model-for-studying-ischemia-reperfusion-injury-of-neuronal-cells
#11
Myoung-Gwi Ryou, Robert T Mallet
Ischemia-reperfusion syndromes of the heart and brain are the leading cause of death and long-term disability worldwide. Development of effective treatments for myocardial infarction, stroke, cardiac arrest and their sequelae requires preclinical models that replicate specific features of ischemia-reperfusion. The complexities of intact animals, including the integrated function of organ systems, autonomic innervation and endocrine factors, often preclude detailed study of specific components of ischemia-reperfusion injury cascades...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29467847/mirna-223-3p-regulates-nlrp3-to-promote-apoptosis-and-inhibit-proliferation-of-hep3b-cells
#12
Lingfeng Wan, Xin Yuan, Mantian Liu, Boyu Xue
Hepatocellular carcinoma (HCC) is a major malignant tumor type with a high incidence and mortality. Infection with hepatitis virus is a high-risk factor. Previous studies have demonstrated that microRNA (miR)-223 was downregulated in HCC tissues. NOD-like receptor family, pyrin domain containing 3 (NLRP3)-is a potential target of miR-223 and has a vital role in hepatitis infection. The present study was performed to investigate the role of miR-223 in the proliferation and apoptosis of HCC cells through regulating NLRP3...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29467561/development-of-a-participatory-capacity-building-program-for-congregational-health-leaders-in-african-american-churches-in-the-us-south
#13
Mercedes M Morales-Alemán, Artisha Moore, Isabel C Scarinci
African Americans are disproportionately affected by diabetes and colorectal cancer. Although studies have shown the effectiveness of spiritually based health interventions delivered by community health workers to African Americans, few have described the development of the capacity-building component. This article describes this process. The development of the Healthy Congregations Healthy Communities Program (HCHC) was guided through a community-based participatory research lens and included: 1) establishment of a community coalition; 2) identification by coalition members of churches as the best venues for health promotion strategies among African Americans; 3) recruitment of churches; 4) development of a training manual; 5) recruitment and training of congregational health leaders (CHLs); and 6) "Passing of the torch" from the coalition to the CHLs who implemented the intervention in their congregations...
2018: Ethnicity & Disease
https://www.readbyqxmd.com/read/29467403/proteome-analysis-of-aspergillus-flavus-isolate-specific-responses-to-oxidative-stress-in-relationship-to-aflatoxin-production-capability
#14
Jake C Fountain, Jin Koh, Liming Yang, Manish K Pandey, Spurthi N Nayak, Prasad Bajaj, Wei-Jian Zhuang, Zhi-Yuan Chen, Robert C Kemerait, R Dewey Lee, Sixue Chen, Rajeev K Varshney, Baozhu Guo
Aspergillus flavus is an opportunistic pathogen of plants such as maize and peanut under conducive conditions such as drought stress resulting in significant aflatoxin production. Drought-associated oxidative stress also exacerbates aflatoxin production by A. flavus. The objectives of this study were to use proteomics to provide insights into the pathogen responses to H2 O2 -derived oxidative stress, and to identify potential biomarkers and targets for host resistance breeding. Three isolates, AF13, NRRL3357, and K54A with high, moderate, and no aflatoxin production, were cultured in medium supplemented with varying levels of H2 O2 , and examined using an iTRAQ (Isobaric Tags for Relative and Absolute Quantification) approach...
February 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29466919/what-qualities-in-a-potential-hiv-pre-exposure-prophylaxis-service-are-valued-by-black-men-who-have-sex-with-men-in-london-a-qualitative-acceptability-study
#15
T Charles Witzel, Will Nutland, Adam Bourne
Black men who have sex with men (BMSM) have higher HIV incidence and prevalence when compared to other MSM, despite similar levels of condom use and testing. Pre-exposure prophylaxis (PrEP) could be a useful intervention to reduce these inequalities. This research therefore aims to understand the dimensions of acceptability of a potential PrEP service for BMSM aged 18-45 years in London. In-depth semi-structured interviews were conducted with 25 PrEP-eligible BMSM between April and August 2016. Interviews were recorded and transcribed verbatim, then subject to a thematic framework analysis, informed by intersectionality theory...
January 1, 2018: International Journal of STD & AIDS
https://www.readbyqxmd.com/read/29466794/foxo1-a-potential-therapeutic-target-regulates-autophagic-flux-oxidative-stress-mitochondrial-dysfunction-and-apoptosis-in-human-cholangiocarcinoma-qbc939-cells
#16
Wei He, Aiqing Zhang, Lei Qi, Chen Na, Rui Jiang, Zhining Fan, Jianping Chen
BACKGROUND/AIMS: Autophagy is an evolutionarily conserved catabolic mechanism to maintain energy homeostasis and to remove damaged cellular components, which plays an important role in the survival of various cells. Inhibiting autophagy is often applied as a new strategy to halt the growth of cancer cells. METHODS: The effect of FOXO1 gene on cellular function and apoptosis and its underlying mechanisms were investigated in cultured QBC939 cells by the methylthiazoletetrazolium (MTT) assay, western blot, DCFDA mitochondrial membrane potential, and ATP content measurement...
February 16, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29463793/aberrant-regulation-of-notch3-signaling-pathway-in-polycystic-kidney-disease
#17
Jessica Idowu, Trisha Home, Nisha Patel, Brenda Magenheimer, Pamela V Tran, Robin L Maser, Christopher J Ward, James P Calvet, Darren P Wallace, Madhulika Sharma
Polycystic kidney disease (PKD) is a genetic disorder characterized by fluid-filled cysts in the kidney and liver that ultimately leads to end-stage renal disease. Currently there is no globally approved therapy for PKD. The Notch signaling pathway regulates cellular processes such as proliferation and de-differentiation, which are cellular hallmarks of PKD. Thus we hypothesized that the Notch pathway plays a critical role in PKD. Evaluation of protein expression of Notch signaling components in kidneys of Autosomal Recessive PKD (ARPKD) and Autosomal Dominant PKD (ADPKD) mouse models and of ADPKD patients revealed that Notch pathway members, particularly Notch3, were consistently upregulated or activated in cyst-lining epithelial cells...
February 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29463625/growth-differentiation-factor-11-promotes-abnormal-proliferation-and-angiogenesis-of-pulmonary-artery-endothelial-cells
#18
Xiufeng Yu, Xinxin Chen, Xiao Dong Zheng, Junting Zhang, Xijuan Zhao, Ying Liu, Hongyue Zhang, Lixin Zhang, Hao Yu, Min Zhang, Cui Ma, Xuewei Hao, Daling Zhu
Disordered proliferation and angiogenesis of pulmonary artery endothelial cells is an important stage in the development of pulmonary arterial hypertension. Recent studies revealed that GDF11 (growth differentiation factor 11) induces endothelial cells proliferation and migration; however, whether GDF11 is directly involved in the pathogenesis of pulmonary arterial hypertension remains unknown. Here, we found that GDF11 was significantly upregulated and activated in 2 experimental pulmonary arterial hypertension models and cultured pulmonary artery endothelial cells...
February 20, 2018: Hypertension
https://www.readbyqxmd.com/read/29463175/developing-a-cultural-context-for-conducting-a-neuropsychological-evaluation-with-a-culturally-diverse-client-the-eclectic-framework
#19
Daryl E M Fujii
OBJECTIVE: With the increasing diversification of the American population, the discipline of neuropsychology is challenged to develop appropriate tools and conceptual models to meet its evolving client base. Thus far, the focus has been on developing appropriate tests and norms to obtain accurate testing data. By contrast, far less attention has been paid to the contextual impact of culture on an evaluation. This paper attempts to address this shortcoming. METHODS: This manuscript introduces the ECLECTIC framework for conceptualizing different facets of culture pertinent for understanding a culturally diverse client when conducting a neuropsychological evaluation...
February 20, 2018: Clinical Neuropsychologist
https://www.readbyqxmd.com/read/29462262/50-years-of-spermatogenesis-sertoli-cells-and-their-interactions-with-germ-cells
#20
Michael D Griswold
The complex morphology of the Sertoli cells and their interactions with germ cells has been a focus of investigators since they were first described by Enrico Sertoli. In the past 50 years, information on Sertoli cells has transcended morphology alone to become increasingly more focused on molecular questions. The goal of investigators has been to understand the role of the Sertoli cells in spermatogenesis and to apply that information to problems relating to male fertility. Sertoli cells are unique in that they are a non-dividing cell population that is active for the reproductive lifetime of the animal and cyclically change morphology and gene expression...
February 15, 2018: Biology of Reproduction
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