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https://www.readbyqxmd.com/read/29667532/human-osteoblast-derived-extracellular-matrix-with-high-homology-to-bone-proteome-is-osteopromotive
#1
Marta Baroncelli, Bram C J van der Eerden, Siddharth Chatterji, Enrique Rull Trinidad, Yik Y Kan, Marijke Koedam, Ingmar A J van Hengel, Rodrigo D A M Alves, Lidy E Fratila-Apachitei, Jeroen A A Demmers, Jeroen van de Peppel, Johannes Ptm van Leeuwen
Efficient osteogenic differentiation of mesenchymal stem cells (MSCs) is crucial to accelerate bone formation. In this context, the use of extracellular matrix (ECM) as natural 3D-framework mimicking in vivo tissue architecture is of interest. The aim of this study was to generate a devitalized human osteogenic MSC-derived ECM and to investigate its impact on MSC osteogenic differentiation to improve MSC properties in bone regeneration. The devitalized ECM significantly enhanced MSC adhesion and proliferation...
April 18, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29666284/biofilms-comprise-a-component-of-the-annual-cycle-of-vibrio-cholerae-in-the-bay-of-bengal-estuary
#2
Marzia Sultana, Suraia Nusrin, Nur A Hasan, Abdus Sadique, Kabir U Ahmed, Atiqul Islam, Anwar Hossain, Ira Longini, Azhar Nizam, Anwar Huq, Abul K Siddique, David A Sack, Richard B Sack, Rita R Colwell, Munirul Alam
Vibrio cholerae , an estuarine bacterium, is the causative agent of cholera, a severe diarrheal disease that demonstrates seasonal incidence in Bangladesh. In an extensive study of V. cholerae occurrence in a natural aquatic environment, water and plankton samples were collected biweekly between December 2005 and November 2006 from Mathbaria, an estuarine village of Bangladesh near the mangrove forests of the Sundarbans. Toxigenic V. cholerae exhibited two seasonal growth peaks, one in spring (March to May) and another in autumn (September to November), corresponding to the two annual seasonal outbreaks of cholera in this region...
April 17, 2018: MBio
https://www.readbyqxmd.com/read/29666269/worldwide-distribution-of-the-dcdc2-read1-regulatory-element-and-its-relationship-with-phoneme-variation-across-languages
#3
Mellissa M C DeMille, Kevin Tang, Chintan M Mehta, Christopher Geissler, Jeffrey G Malins, Natalie R Powers, Beatrice M Bowen, Andrew K Adams, Dongnhu T Truong, Jan C Frijters, Jeffrey R Gruen
DCDC2 is a gene strongly associated with components of the phonological processing system in animal models and in multiple independent studies of populations and languages. We propose that it may also influence population-level variation in language component usage. To test this hypothesis, we investigated the evolution and worldwide distribution of the READ1 regulatory element within DCDC2 , and compared its distribution with variation in different language properties. The mutational history of READ1 was estimated by examining primate and archaic hominin sequences...
April 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29666193/lmo1656-is-a-secreted-virulence-factor-of-listeria-monocytogenes-that-interacts-with-the-sortin-nexin-6-bar-complex
#4
Daryl Jason David, Alessandro Pagliuso, Lilliana Radoshevich, Marie-Anne Nahori, Pascale Cossart
Listeria monocytogenes (Lm) is a facultative intracellular bacterial pathogen, the causative agent of listeriosis, a rare but fatal disease.  During infection, Lm can traverse several physiological barriers; it can cross the intestine and placenta, and in immunocompromised individuals, the blood-brain barrier. With the recent plethora of sequenced genomes available for Lm, it is clear that the complete repertoire of genes used by Lm to interact with its host remains to be fully explored.  Recently, we focused on secreted Lm proteins, since they are likely to interact with host cell components...
April 17, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29665831/sexual-and-reproductive-health-services-utilization-and-associated-factors-among-secondary-school-students-in-nekemte-town-ethiopia
#5
Wakgari Binu, Taklu Marama, Mulusew Gerbaba, Melese Sinaga
BACKGROUND: Despite policy actions and strategic efforts made to promote sexual and reproductive health service uptake of youths in Ethiopia, its utilization remains very low and little information was found on the extent to which school youths utilize available reproductive health services in Nekempt town. This study was aimed to assess utilization of Sexual and Reproduactive Health (SRH) services and its associated factors among secondary school students in Nekemte town, Ethiopia. METHOD: A school based cross-sectional study design was conducted from April 18 to 22, 2016...
April 17, 2018: Reproductive Health
https://www.readbyqxmd.com/read/29665822/toxoplasma-gondii-dense-granule-protein-15-induces-apoptosis-in-choriocarcinoma-jeg-3-cells-through-endoplasmic-reticulum-stress
#6
Wei Wei, Fangfang Zhang, He Chen, Yuanyuan Tang, Tian Xing, Qingli Luo, Li Yu, Jian Du, Jilong Shen, Linjie Zhang
BACKGROUND: Toxoplasma gondii, a single-celled parasite commonly found in mammals, has been shown to induce trophoblast cell apoptosis and subsequently cause fetal damage and abortion. Although dense granule protein 15 (GRA15) has been identified as a key component in innate immunity to T. gondii infection and its pathogenesis, its role in host cell apoptosis remains unclarified. METHODS: Type II GRA15 (GRA15II ) cDNA was inserted into a plasmid encoding enhanced green fluorescent protein (pEGFP)...
April 17, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29665703/culturally-safe-health-initiatives-for-indigenous-peoples-in-canada-a-scoping-review
#7
Lauren Brooks-Cleator, Breanna Phillipps, Audrey Giles
Background Cultural safety has the potential to improve the health disparities between Indigenous and non-Indigenous Canadians, yet practical applications of the concept are lacking in the literature. Purpose This study aims to identify the key components of culturally safe health initiatives for the Indigenous population of Canada to refine its application in health-care settings. Methods We conducted a scoping review of the literature pertaining to culturally safe health promotion programs, initiatives, services, or care for the Indigenous population in Canada...
January 1, 2018: Canadian Journal of Nursing Research, Revue Canadienne de Recherche en Sciences Infirmières
https://www.readbyqxmd.com/read/29665451/tgf-%C3%AE-1-resulting-in-differential-microrna-expression-in-bovine-granulosa-cells
#8
Yefen Xu, Jiaqiang Niu, Guangying Xi, Xuezhi Niu, Yuheng Wang, Ming Guo, Qiangba Yangzong, Yilong Yao, Suolang Sizhu, Jianhui Tian
To explore the expression profile of the cellular miRNAs in bovine ovarian granulosa cells responding to transforming growth factor-β1 (TGF-β1), the effect of TGF-β1 on cell proliferation was firstly investigated by CCK-8 method and the results showed that there was a significant inhibitory effect on bovine granulosa cell proliferation treated with 5/10 ng/mL human recombinant TGF-β1 for 24 h compared to the control (P < 0.05). Then, we performed high-throughput sequencing of two small RNA libraries prepared from cultured bovine granulosa cells stimulated with or without 10 ng/mL human recombinant TGF-β1...
April 14, 2018: Gene
https://www.readbyqxmd.com/read/29665277/the-expression-of-endogenous-voltage-gated-potassium-channels-in-hek293-cells-is-affected-by-culture-conditions
#9
Arturo Ponce, Aida Castillo, Lorena Hinojosa, Jacqueline Martinez-Rendon, Marcelino Cereijido
HEK293 cells are widely used as a host for expression of heterologous proteins; yet, little care has been taken to characterize their endogenous membrane components, including ion channels. In this work, we aimed to describe the biophysical and pharmacological properties of endogenous, voltage-dependent potassium currents (IKv). We also examined how its expression depends on culture conditions. We used the electrophysiological technique of whole-cell patch clamp to record ion currents from HEK293 cells. We found that HEK cells express endogenous, voltage-dependent potassium currents...
April 2018: Physiological Reports
https://www.readbyqxmd.com/read/29665228/ultra-high-field-fmri-insights-on-insight-neural-correlates-of-the-aha-moment
#10
Martin Tik, Ronald Sladky, Caroline Di Bernardi Luft, David Willinger, André Hoffmann, Michael J Banissy, Joydeep Bhattacharya, Christian Windischberger
Finding creative solutions to difficult problems is a fundamental aspect of human culture and a skill highly needed. However, the exact neural processes underlying creative problem solving remain unclear. Insightful problem solving tasks were shown to be a valid method for investigating one subcomponent of creativity: the Aha!-moment. Finding insightful solutions during a remote associates task (RAT) was found to elicit specific cortical activity changes. Considering the strong affective components of Aha!-moments, as manifested in the subjectively experienced feeling of relief following the sudden emergence of the solution of the problem without any conscious forewarning, we hypothesized the subcortical dopaminergic reward network to be critically engaged during Aha...
April 17, 2018: Human Brain Mapping
https://www.readbyqxmd.com/read/29664975/estimation-of-unobservable-selection-effects-in-on-line-surveys-through-propensity-score-matching-an-application-to-public-acceptance-of-healthy-eating-policies
#11
Sara Capacci, Mario Mazzocchi, Sergio Brasini
The use of model-based propensity scores as matching tools opens the way to the indirect estimation of mode-related measurement effects and selection effects in web surveys, including a component of selection that cannot be traced back to observable characteristics. By matching and comparing respondents from real independent surveys that use the same questionnaire, but different administration modes, it becomes possible to isolate the selection effect induced by unobservable (or unobserved) respondent characteristics...
2018: PloS One
https://www.readbyqxmd.com/read/29664223/chondrogenic-response-in-presence-of-cartilage-extracellular-matrix-nanoparticles
#12
Saeed Zahiri, Elahe Masaeli, Elahe Poorazizi, Mohammad Hossein Nasr-Esfahani
Current studies based on regenerative medicine suggest, decellularized extracellular matrix (DC-ECM) components can regulate cell phenotype. In this regard, it is believed, presence of cartilage extracellular matrix particles in culture condition could produce physical and biochemical supportive cues for chondrogenesis. In this study, DC-ECM nanoparticles with average size of 61.5± 22.4 nm were produced by decellularization and mechanical processing. Homogenous distribution and nano scale size of yield particles were observed by Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) microscopy imaging...
April 17, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29662124/stag2-deficiency-induces-interferon-responses-via-cgas-sting-pathway-and-restricts-virus-infection
#13
Siyuan Ding, Jonathan Diep, Ningguo Feng, Lili Ren, Bin Li, Yaw Shin Ooi, Xin Wang, Kevin F Brulois, Linda L Yasukawa, Xingnan Li, Calvin J Kuo, David A Solomon, Jan E Carette, Harry B Greenberg
Cohesin is a multi-subunit nuclear protein complex that coordinates sister chromatid separation during cell division. Highly frequent somatic mutations in genes encoding core cohesin subunits have been reported in multiple cancer types. Here, using a genome-wide CRISPR-Cas9 screening approach to identify host dependency factors and novel innate immune regulators of rotavirus (RV) infection, we demonstrate that the loss of STAG2, an important component of the cohesin complex, confers resistance to RV replication in cell culture and human intestinal enteroids...
April 16, 2018: Nature Communications
https://www.readbyqxmd.com/read/29660523/inhibition-of-quorum-sensing-mediated-virulence-in-serratia-marcescens-by-bacillus-subtilis-r-18
#14
Kannan Rama Devi, Subramaniyan Srinivasan, Arumugam Veera Ravi
Serratia marcescens is an opportunistic human pathogen causing various nosocomial infections, most importantly urinary tract infections (UTIs). It exhibits increased resistance towards the conventional antibiotics. This study was aimed to evaluate the anti-virulence effect of a rhizosphere soil bacterium Bacillus subtilis strain R-18 against the uropathogen S. marcescens. First, the bacterial cell-free culture supernatant (CFCS) of B. subtilis strain R-18 was evaluated for its quorum sensing inhibitory (QSI) potential against biomarker strain Chromobacterium violaceum and the test pathogen S...
April 13, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29660413/validation-of-french-upper-limb-erasmus-modified-nottingham-sensory-assessment-in-stroke
#15
Claire Villepinte, Emilie Catella, Magali Martin, Sylvie Hidalgo, Sabrina Téchené, Claire Lebely, Evelyne Castel-Lacanal, Xavier de Boissezon, HuiJun Chih, David Gasq
BACKGROUND: Somatosensory impairment of the upper limb (UL) occurs in approximately 50% of adults post-stroke, associated with loss of hand motor function, activity and participation. Measurement of UL sensory impairment is a component of rehabilitation contributing to the selection of sensorimotor techniques optimising recovery and providing a prognostic estimate of UL function. To date, no standardized official French version of a measure of somatosensory impairment has been established...
April 13, 2018: Annals of Physical and Rehabilitation Medicine
https://www.readbyqxmd.com/read/29660082/molecular-components-of-arabidopsis-intact-vacuoles-clarified-with-metabolomic-and-proteomic-analyses
#16
Miwa Ohnishi, Aya Anegawa, Yuko Sugiyama, Kazuo Harada, Akira Oikawa, Yasumune Nakayama, Fumio Matsuda, Yukiko Nakamura, Ryosuke Sasaki, Chizuko Shichijo, Patrick G Hatcher, Hidehiro Fukaki, Shigehiko Kanaya, Koh Aoki, Mami Yamazaki, Eiichiro Fukusaki, Kazuki Saito, Tetsuro Mimura
We analyzed the metabolites and proteins contained in pure intact vacuoles isolated from Arabidopsis suspension cultured cells using CE (capillary electrophoresis)-MS, FT-ICR (Fourier transform-ion cyclotron resonance)-MS and LC (Liquid chromatography)-MS. We identified 19 amino acids and 5 organic acids as major primary metabolites in the vacuoles with CE-MS. Further, we identified small amounts of 27 substances including well-known vacuolar molecules, but also some unexpected substances (for example, organic phosphate compounds)...
April 12, 2018: Plant & Cell Physiology
https://www.readbyqxmd.com/read/29659590/organic-cultivation-of-ashwagandha-with-improved-biomass-and-high-content-of-active-withanolides-use-of-vermicompost
#17
Amandeep Kaur, Baldev Singh, Puja Ohri, Jia Wang, Renu Wadhwa, Sunil C Kaul, Pratap Kumar Pati, Arvinder Kaur
Withania somnifera (Ashwagandha) has recently been studied extensively for its health-supplementing and therapeutic activities against a variety of ailments. Several independent studies have experimentally demonstrated pharmaceutical potential of its active Withanolides, Withaferin A (Wi-A), Withanone (Wi-N) and Withanolide A (Wil-A). However, to promote its use in herbal industry, an environmentally sustainable cultivation and high yield are warranted. In modern agriculture strategies, there has been indiscriminate use of chemical fertilizers to boost the crop-yield, however the practice largely ignored its adverse effect on the quality of soil and the environment...
2018: PloS One
https://www.readbyqxmd.com/read/29659028/the-classroom-environment-questionnaire-ceq-development-and-preliminary-structural-validity
#18
Carissa Lyons, Ted Brown, Helen Bourke-Taylor
BACKGROUND/AIM: Occupational therapists offer a unique perspective regarding the contribution of the environment to occupational performance. Therefore, a scale that measures the unique characteristics of the primary school classroom environment where children complete their daily schoolwork occupations is needed. The aim of this study was to develop and psychometrically evaluate a new teacher-report questionnaire that measures a number of environmental characteristics of primary school classrooms...
April 16, 2018: Australian Occupational Therapy Journal
https://www.readbyqxmd.com/read/29658852/evidence-based-practice-making-the-research-relevant-to-your-situation-clue-4-apply
#19
Catherine F Yonkaitis, Erin D Maughan
Evidence-based practice (EBP) is often thought to be synonymous with research and literature. This article focuses on the fourth step in the EBP process: Apply. In this step, we fully integrate the EBP Venn diagram, which illustrates that EBP occurs at the intersection of evidence and data, clinical expertise and resources, and population's values and cultures. Only when school nurses include each component into their practice decision will true EBP occur.
May 2018: NASN School Nurse
https://www.readbyqxmd.com/read/29658273/dual-role-of-humic-substances-as-electron-donor-and-shuttle-for-dissimilatory-iron-reduction
#20
Noah Stern, Jacqueline Mejia, Shaomei He, Yu Yang, Matthew Ginder-Vogel, Eric E Roden
Dissimilatory iron-reducing bacteria (DIRB) are known to use humic substances (HS) as electron shuttles for dissimilatory iron reduction (DIR) by transferring electrons to HS-quinone moieties, which in turn rapidly reduce Fe(III) oxides. It has also been proposed that certain components of HS can be mineralized by microorganisms. However, the potential for HS to serve a source of organic carbon (OC) that fuels DIR is unknown. We studied whether isolated humic acids (HA) and humins (HM) could serve as both electron shuttles and electron donors for DIR by freshwater sediment microorganisms...
April 16, 2018: Environmental Science & Technology
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