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https://www.readbyqxmd.com/read/29050459/third-party-regulatory-t-cells-prevent-murine-acute-graft-versus-host-disease
#1
Jung-Yeon Lim, Keon-Il Im, Yunejin Song, Nayoun Kim, Young-Sun Nam, Young-Woo Jeon, Seok-Goo Cho
Background/Aims: Adoptive therapy with regulatory T (Treg) cells to prevent graft-versus-host disease (GVHD) would benefit from a strategy to improve homing to the sites of inflammation following hematopoietic stem cell transplantation (HSCT). Although donor-derived Treg cells have mainly been used in these models, third-party-derived Treg cells are a promising alternative for cell-based immunotherapy, as they can be screened for pathogens and cell activity, and banked for GVHD prevention...
October 19, 2017: Korean Journal of Internal Medicine
https://www.readbyqxmd.com/read/29050446/management-of-mycoses-in-daily-practice
#2
Lorenzo Drago, Giuseppe Micali, Manuela Papini, Bianca M Piraccini, Stefano Veraldi
The guideline recommendations, albeit founded on thorough reviews of clinically relevant literature data, are often not immediately adaptable to everyday life. Considering the marked heterogeneity of superficial mycoses, each of them requires specific management in a real life context; in all cases diagnostic confirmation is required with microscopic and culture examination. In tinea capitis oral therapy is necessary (minimum six weeks) and should be continued until clinical and, above all, mycological healing...
December 2017: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
https://www.readbyqxmd.com/read/29050353/combined-efficacy-of-c-reactive-protein-and-red-blood-cell-distribution-width-in-prognosis-of-patients-with-culture-negative-infective-endocarditis
#3
Xue-Biao Wei, Yuan-Hui Liu, Peng-Cheng He, Ying-Ling Zhou, Ning Tan, Ji-Yan Chen, Dan-Qing Yu
OBJECTIVE: To evaluate the combined effect of C-reactive protein (CRP) and red blood cell distribution width (RDW) on the prediction of in-hospital and long-term poor outcomes in patients with blood culture-negative infective endocarditis (BCNE). RESULTS: Patients with high CRP and high RDW has the highest incidence of in-hospital death (2.3% vs. 7.8% vs. 5.6% vs. 17.5%, P < 0.001). CRP > 17.8 mg/L (odds ratio [OR]=2.41, 95% confidence interval [CI], 1.06-5...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050240/total-flavones-of-abelmoschus-manihot-enhances-angiogenic-ability-both-in-vitro-and-in-vivo
#4
Lingyi Tang, Wu Pan, Guisong Zhu, Zhihui Liu, Dongling Lv, Meng Jiang
Angiogenesis is a process of new blood vessel formation from pre-existing vessels. It is a normal and vital process in growth and development, as well as in wound healing and in the formation of granulation tissue. Total flavones of Abelmoschus manihot (TFA) are the major constituents of the traditional Chinese herb Abelmoschus manihot L. Medic. The aim of this study is to investigate the effect of TFA on angiogenic ability using human umbilical vein endothelial cells (HUVECs) in vitro and chick chorioallantoic membrane (CAM) in vivo...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050115/-effects-of-ovalbumin-exposure-during-pregnancy-of-mice-on-the-ovalbumin-re-exposure-in-adult-progeny
#5
Z Song, H Q Li
Objective: To observe the immunoreaction of offspring mice by ovalbumin (OVA) re-exposure after their mothers exposed to OVA during pregnancy. Method: A prospective controlled study was conducted to observe mice after repeated OVA exposures at 6-8 weeks.Their mothers were exposed to OVA during different stages of pregnancy.The symptoms were recorded and scored.The levels of OVA-specific IgE in serum, interferon-γ(IFN-γ) and interleukin-4(IL-4) in supernatant of spleen primary lymphocytes in vitro were measured by ELISA...
October 2, 2017: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
https://www.readbyqxmd.com/read/29050034/mir-29b-regulates-oxidative-stress-by-targeting-sirt1-in-ovarian-cancer-cells
#6
Meng Hou, Xiaohang Zuo, Chen Li, Yan Zhang, Yue Teng
BACKGROUND: Metabolic abnormalities are frequently observed in multiple malignancies including epithelial ovarian cancer (EOC), among which imbalance between generation and elimination of reactive oxygen species (ROS) plays a critical role in EOC onset and progression. Here we investigated the role of miR-29b, a well-established tumor-suppressor miRNA in metabolic regulation of EOC cells. METHODS: cell viability and apoptosis in miR-29b inhibited and over-expressed EOC cells were evaluated by CCK8 and Annexin V-FITC/PI assays...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29050010/calcium-sensing-receptor-stimulation-in-cultured-glomerular-podocytes-induces-trpc6-dependent-calcium-entry-and-rhoa-activation
#7
Lei Zhang, Tianrong Ji, Qin Wang, Kexin Meng, Rui Zhang, He Yang, Chang Liao, Linlin Ma, Jundong Jiao
BACKGROUND/AIMS: Recent studies provided compelling evidence that stimulation of the calcium sensing receptor (CaSR) exerts direct renoprotective action at the glomerular podocyte level. This protective action may be attributed to the RhoA-dependent stabilization of the actin cytoskeleton. However, the underlying mechanisms remain unclear. METHODS: In the present study, an immortalized human podocyte cell line was used. Fluo-3 fluorescence was utilized to determine intracellular Ca2+ concentration ([Ca2+]i), and western blotting was used to measure canonical transient receptor potential 6 (TRPC6) protein expression and RhoA activity...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049996/nogo-b-promotes-angiogenesis-in-proliferative-diabetic-retinopathy-via-vegf-pi3k-akt-pathway-in-an-autocrine-manner
#8
Yuelu Zhang, Liang Wang, Yuechan Zhang, Mo Wang, Qinglei Sun, Fangzhou Xia, Ruobing Wang, Lin Liu
BACKGROUND/AIMS: Nogo-B, a conservative protein of endoplasmic reticulum, is a member of the reticulon family of proteins. Proliferative diabetic retinopathy (PDR) is the major concerning problem of diabetic retinopathy. This study explored the role of Nogo-B in the regulation of angiogenesis in PDR patients and primary human retinal endothelial cells (HRMECs). METHODS: Nogo-B was down-regulated through the use of Lentivirus-NogoB-RNAi, the effects of Nogo-B on angiogenesis under high glucose stimulation were evaluated via CCK-8 assay, wound closure assay, transwell assay, and tube formation assay...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049988/magnolia-officinalis-bark-extract-induces-depolarization-of-pacemaker-potentials-through-m2-and-m3-muscarinic-receptors-in-cultured-murine-small-intestine-interstitial-cells-of-cajal
#9
Hyun Jung Kim, Taewon Han, Yun Tai Kim, Insuk So, Byung Joo Kim
BACKGROUND: Magnolia officinalis Rehder and EH Wilson (M. officinalis) are traditional Chinese medicines widely used for gastrointestinal (GI) tract motility disorder in Asian countries. We investigated the effects of an ethanol extract of M. officinalis (MOE) on the pacemaker potentials of cultured interstitial cells of Cajal (ICCs) in vitro and its effects on GI motor functions in vivo. METHODS: We isolated ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record the pacemaker potentials in cultured ICCs in vitro...
October 19, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29049954/application-of-carnobacterium-maltaromaticum-as-a-feed-additive-for-weaned-rabbits-to-improve-meat-microbial-quality-and-safety
#10
Amenan Prisca Koné, Juliana Maria Velez Zea, Dominic Gagné, Dany Cinq-Mars, Frédéric Guay, Linda Saucier
This study addresses the improvement of meat microbial quality by enriching the diet of farm animals with a protective culture. Weaned Grimaud rabbits were divided into two experimental groups: a control and a diet supplemented with Micocin® (Carnobacterium maltaromaticum CB1; 8Log10CFU/kg of feed). Overall, meat quality was not affected substantially by the treatment. Total Aerobic Mesophilic (TAM), Escherichia coli and other coliforms, Enterobacteriaceae, Staphylococcus aureus, Pseudomonas spp., Listeria spp...
October 2, 2017: Meat Science
https://www.readbyqxmd.com/read/29049940/photocatalytic-degradation-of-synthetic-food-dye-sunset-yellow-fcf-fd-c-yellow-no-6-by-ailanthus-excelsa-roxb-possessing-antioxidant-and-cytotoxic-activity
#11
Subramanyam Deepika, Rajendran Harishkumar, Murugesan Dinesh, Rajadurai Abarna, Moorthy Anbalagan, Selvaraj Mohana Roopan, Chinnadurai Immanuel Selvaraj
The purpose of our work is to identify the bioactive compounds of bark and leaves extract from Ailanthus excelsa Roxb. and to explore its effectiveness against synthetic food dye. The presence of primary and secondary metabolites was confirmed by carrying out phytochemicals analysis. With the prior knowledge accessible on the indispensable secondary metabolites holding antioxidant and cytotoxicity activity, the quantitative screening of total phenolic and flavonoid content in methanolic and aqueous extract of bark and leaves from Ailanthus excelsa were done...
October 12, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29049939/water-assessment-using-ultra-weak-bioluminescence
#12
A C Cordeiro, J L Fabris, G H Couto, H J Kalinowski, E Bertogna
In this paper a method to evaluate the presence of microorganisms of the coliform group in water samples using the ultra-weak bioluminescence (UWB) is proposed. A series of UWB measurements and optical density measurements from cultures of both a set of standard E. coli strain samples, and a set of water samples from a river near Curitiba City in Brazil were performed. All samples were previously incubated at 37°C for 11h in nutritive medium before the temporal UWB emission profiles data were acquired for a period of 24h inside a dark chamber of an especially implemented instrumentation capable of doing photon counting measurements...
October 10, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/29049926/afm-detected-apoptosis-of-hepatocellular-carcinoma-cells-induced-by-american-ginseng-root-water-extract
#13
Yingmin Qu, Zuobin Wang, Feihu Zhao, Jinyun Liu, Wenxiao Zhang, Jingmei Li, Zhengxun Song, Hongmei Xu
American ginseng as a common and traditional herbal medicine has been used in cancer treatment for many years. However, the effect of American ginseng on the cancer cell response (i.e. apoptosis) has not been fully understood yet. Previous studies demonstrated that cellular apoptosis was associated with the changes of mechanical and morphological properties. Therefore, in this study, mechanical and morphological characterizations were carried out by both atomic force microscope (AFM) and inverted optical microscope to investigate the apoptosis of hepatocellular carcinoma (SMMC-7721) cells affected by American ginseng root water extract (AGRWE)...
October 12, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/29049922/effect-of-co-culture-canine-cumulus-and-oviduct-cells-with-porcine-oocytes-during-maturation-and-subsequent-embryo-development-of-parthenotes-in%C3%A2-vitro
#14
Seok Hee Lee, Hyun Ju Oh, Min Jung Kim, Geon A Kim, Yoo Bin Choi, Young Kwang Jo, Erif Maha Nugraha Setyawan, Byeong Chun Lee
In the estrus stage, canine oocytes are surrounded by cumulus cells and undergo maturation in the oviduct for 2-3 days after ovulation. We hypothesized that canine oviduct cells (cOC) and canine cumulus cells (cCC) during this stage might affect the maturation of oocytes and thereby improve subsequent embryo development. Therefore, the objective of this study was to compare the effects of a cOC and cCC co-culture on oocyte in vitro maturation (IVM) and subsequent embryo development, and to analyze the gene expressions in a molecular fashion what co-culture actually gives the specific pathways in which the co-culture cells act to improve maturation and embryo development...
October 3, 2017: Theriogenology
https://www.readbyqxmd.com/read/29049722/a-ppar-gamma-agonist-rosiglitazone-suppresses-fibrotic-response-in-human-pterygium-fibroblasts-by-modulating-the-p38-mapk-pathway
#15
Selikem Abla Nuwormegbe, Joon Hyung Sohn, Sun Woong Kim
Purpose: Fibroblast activation may play an important role in pterygium progression. Synthetic peroxisome proliferator-activated receptor γ (PPAR-γ) ligands have been shown to be effective antifibrotic agents against transforming growth factor β1 (TGF-β1) induced fibrosis in several tissues. We aimed to investigate the antifibrotic effects of the PPAR-γ ligand rosiglitazone in pterygium fibroblasts and the underlying mechanisms. Methods: Profibrotic activation was induced by TGF-β1 in primary cultured human pterygium fibroblasts and the effect of rosiglitazone treatment on α-smooth muscle actin (α-SMA), and extra cellular matrix proteins synthesis was detected by western blotting, real-time PCR, immunostaining, and flow cytometry...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29049314/evaluation-of-characteristic-of-human-turbinate-derived-mesenchymal-stem-cells-cultured-in-the-serum-free-media
#16
Se Hwan Hwang, WeonSun Lee, Sang Hi Park, Hee Jin Lee, Sun Hwa Park, Dong Chang Lee, Mi Hyun Lim, Sang A Back, Byeong Gon Yun, Jung Ho Jeun, Jung Yeon Lim, Jun Myung Kang, Sung Won Kim
We evaluated the effect of serum-free and xeno-cultivation (SFXFM) on the characterization, proliferation, and differentiation properties of human nasal stem cells (airway tissue; hTMSCs). hTMSCs were isolated from 10 patients, after which patient samples were separated into two groups, an SFXFM group and a control group. The control group was treated with bovine serum-containing medium. FACS analysis revealed that SFXFM-cultured hTMSCs maintained a characteristic mesenchymal stem cell phenotype. hTMSC proliferation was not influenced by SFXFM...
2017: PloS One
https://www.readbyqxmd.com/read/29049191/significance-of-monitoring-plasma-concentration-of-voriconazole-in-a-patient-with-liver-failure-a-case-report
#17
Xiaoyan Liu, Haibin Su, Jingjing Tong, Jing Chen, Haozhen Yang, Long Xiao, Jinhua Hu, Lina Zhang
RATIONALE: Invasive pulmonary aspergillosis is associated with significant morbidity and mortality in patients with liver failure. Voriconazole (VRCZ) is recommended as a primary therapeutic agent for the treatment of invasive aspergillosis and metabolized in the liver. Now, data are still lacking on the safety and appropriate dosage of VRCZ in patients with liver failure. Here, we report a representative case of invasive pulmonary fungal infection in a patient with liver failure who was treated with low-dose VRCZ...
October 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29048965/the-effectiveness-of-hiv-prevention-interventions-in-socioeconomically-disadvantaged-ethnic-minority-women-a-systematic-review-and-meta-analysis
#18
Isabel Ruiz-Perez, Matthew Murphy, Guadalupe Pastor-Moreno, Antonio Rojas-García, Miguel Rodríguez-Barranco
BACKGROUND: Surveys in the United States and Europe have shown a plateau of new HIV cases, with certain regions and populations disproportionately affected by the disease. Ethnic minority women and socioeconomically disadvantaged groups are disproportionately affected by HIV. Previous reviews have focused on prevention interventions targeting ethnic minority men who have sex with men, have not accounted for socioeconomic status, or have included only interventions carried out in clinical settings...
October 19, 2017: American Journal of Public Health
https://www.readbyqxmd.com/read/29048950/integrating-population-heterogeneity-indices-with-microfluidic-cell-based-assays
#19
Thomas A Moore, Alexander Li, Edmond W K Young
Recent advances in cell-based assays have involved the integration of single-cell analyses and microfluidics technology to facilitate both high-content and high-throughput applications. These technical advances have yielded large datasets with single-cell resolution, and have given rise to the study of cell population dynamics, but statistical analyses of these populations and their properties have received much less attention, particularly for cells cultured in microfluidic systems. The objective of this study was to perform statistical analyses using Pittsburgh Heterogeneity Indices (PHIs) to understand the heterogeneity and evolution of cell population demographics on datasets generated from a microfluidic single-cell-resolution cell-based assay...
October 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/29048783/-investigation-on-silymarin-impact-on-lipopolysaccharide-induced-inflammation-model-based-on-arachidonic-acid-metabolism-pathway
#20
Danti Mai, Chan Yang, Yun Xue, Yan Wang, Chao Yan
The objective of this research is to investigate the suppressive effect of silymarin on vitro cell culture model of inflammatory macrophage RAW264.7 induced by Kdo2-Lipid A, and explore its mechanism based on cell metabonomics. Ultra-high performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) method was used in the cell metabonomic assay to quantitative analysis of metabolites related to eicosanoids pathway. Then chemometric approaches such as principal component analysis were used to process the metabolic data...
June 8, 2017: Se Pu, Chinese Journal of Chromatography
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