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https://www.readbyqxmd.com/read/29458221/thyroid-stimulating-hormone-stimulated-human-adipocytes-express-thymic-stromal-lymphopoietin
#1
Loretta Ma, AnneMarie Gagnon, Anne Landry, Timothea Le, Fengxia Xiao, Cathy Sun, Heather A Lochnan, Dylan Burger, Alexander Sorisky
When recombinant human (rh) thyroid-stimulating hormone (TSH) is administered to thyroid cancer survivors, an acute extra-thyroidal effect raises pro-inflammatory cytokines and activates platelets. Thymic stromal lymphopoietin (TSLP) is a cytokine recently implicated in platelet activation. Our aim was to measure platelet microparticle levels after rhTSH stimulation in vivo, and to investigate TSLP expression in TSH-stimulated human adipocytes in culture. Blood samples for total and platelet microparticle analysis were obtained from thyroid cancer survivors before (day 1) and after rhTSH administration (day 5)...
February 19, 2018: Hormone and Metabolic Research, Hormon- und Stoffwechselforschung, Hormones et Métabolisme
https://www.readbyqxmd.com/read/29458149/age-dependent-oxidation-of-extracellular-cysteine-cystine-redox-state-e-h-cys-cyss-in-mouse-lung-fibroblasts-is-mediated-by-a-decline-in-slc7a11-expression
#2
Yuxuan Zheng, Jeffrey D Ritzenthaler, Tom J Burke, Javier Otero, Jesse Roman, Walter H Watson
Aging is associated with progressive oxidation of the extracellular environment. The redox state of human plasma, defined by the concentrations of cysteine (Cys) and cystine (CySS), becomes more oxidized as we age. Recently, we showed that fibroblasts isolated from the lungs of young and old mice retain this differential phenotype; old cells produce and maintain a more oxidizing extracellular redox potential (Eh (Cys/CySS)) than young cells. Microarray analysis identified down-regulation of Slc7a11, the light subunit of the CySS/glutamate transporter, as a potential mediator of age-related oxidation in these cells...
February 16, 2018: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29458137/bisphenol-a-alters-oocyte-maturation-by-prematurely-closing-gap-junctions-in-the-cumulus-cell-oocyte-complex
#3
Deyanira Guadalupe Acuña-Hernández, Laura Arreola-Mendoza, Ramsés Santacruz-Márquez, Sihomara Patricia García-Zepeda, Lyda Yuliana Parra-Forero, Jesús Alberto Olivares-Reyes, Isabel Hernández-Ochoa
In ovarian follicles, cumulus cells communicate with the oocyte through gap junction intercellular communication (GJIC), to nurture the oocyte and control its meiosis arrest and division. Bisphenol A (BPA) is a monomer found in polycarbonate-made containers that can induce functional alterations, including impaired oocyte meiotic division and reduced molecule transfer in GJIC. However, how BPA alters oocyte meiotic division is unclear. We investigated whether BPA effects on oocyte meiotic division were correlated with reduced transfer in GJIC...
February 16, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29458086/characterization-of-uniaxial-high-speed-stretch-as-an-in-vitro-model-of-mild-traumatic-brain-injury-on-the-blood-brain-barrier
#4
Hector Rosas-Hernandez, Elvis Cuevas, Claudia Escudero-Lourdes, Susan M Lantz, Nasya M Sturdivant, Syed Z Imam, Sumit Sarkar, William Slikker, Merle G Paule, Kartik Balachandran, Syed F Ali
Traumatic brain injury (TBI) occurs when external mechanical forces induce brain damage as result of impact, penetration or rapid acceleration/deceleration that causes deformation of brain tissue. Depending on its severity, TBI can be classified as mild, moderate or severe and can lead to blood-brain barrier (BBB) dysfunction. In the present study, we evaluated the effects of uniaxial high-speed stretch (HSS) at 0, 5, 10 and 15% on a pure culture of primary rat brain endothelial cells as an in vitro model of TBI to the BBB...
February 16, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29458077/reciprocal-regulation-of-the-cadherin-11-stat3-axis-by-caveolin-1-in-mouse-fibroblasts-and-lung-carcinoma-cells
#5
M Geletu, R Mohan, R Arulanandam, A Berger-Becvar, I R Nabi, P T Gunning, L Raptis
Caveolin-1 (Cav1) is an integral plasma membrane protein and a complex regulator of signal transduction. The Signal Transducer and Activator of Transcription-3 (Stat3) is activated by a number of receptor and non-receptor tyrosine kinases and is positively implicated in cancer. Despite extensive efforts, the relationship between Cav1 and Stat3 has been a matter of controversy. We previously demonstrated that engagement of E- or N-cadherin or cadherin-11 cell to cell adhesion molecules, as occurs with confluence of cultured cells, triggers a dramatic increase in the levels of tyr705 phosphorylated ie activated Stat3, by a mechanism requiring the cRac1 small GTPase...
February 16, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29458072/influence-of-xenorhabdus-gamma-proteobacteria-enterobacteriaceae-symbionts-on-gonad-postembryonic-development-in-steinernema-nematoda-steinernematidae-nematodes
#6
Alexandra C Roder, S Patricia Stock
Steinernema nematodes and their Xenorhabdus partners form an obligate mutualistic association. This partnership is insecticidal to a wide range of insects. Steinernema rely on their Xenorhabdus partner to produce toxins inside the insect cadaver to liberate nutrients from the insect, as well as antimicrobials to sterilize the cadaver, thus creating a suitable environment for reproduction. In return, Steinernema vector their Xenorhabdus between insect hosts. Disruption of this partnership may affect the success of both partners...
February 16, 2018: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/29458025/cell-model-for-the-identification-and-characterization-of-prion-like-components-from-alzheimer-brain-tissue
#7
Daniel Markx, Cornelia Loos, Stephanie Claus, Christian Haupt, Christian Mawrin, Marcus Fändrich
Intracerebral injection of brain extracts from Alzheimer's disease (AD) patients into appropriate mouse models was previously found to drastically accelerate the deposition of Aβ amyloid in the recipient animals indicating a prion-like activity. In this study we show that this prion-like activity can be also identified by using a cell culture model of Aβ plaque formation. Analysis of biochemical fractions of AD brain extract indicate that the seeding-activity correlated with the presence of Aβ peptide and Aβ-derived aggregates...
February 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29458007/a-genomically-characterized-collection-of-high-grade-serous-ovarian-cancer-xenografts-for-preclinical-testing
#8
Paulina Cybulska, Jocelyn M Stewart, Azin Sayad, Carl Virtanen, Patricia A Shaw, Blaise Clarke, Natalie Stickle, Marcus Q Bernardini, Benjamin G Neel
High-grade serous ovarian cancer (HGSC) is the leading cause of morbidity and mortality from gynecologic malignancy. Overall survival remains low, due to the nearly ubiquitous emergence of platinum-resistance and the paucity of effective next-line treatments. Current cell culture-based models show limited similarity to HGSC and are therefore unreliable predictive models for pre-clinical evaluation of investigational drugs. This deficiency could help explain the low overall rate of successful drug development and the decades of largely unchanged approaches to HGSC treatment...
February 16, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29457915/how-good-are-we-at-selecting-students-that-meet-our-mission-outcomes-of-the-2011-and-2012-entering-classes-selected-by-a-locally-developed-multiple-mini-interview
#9
Carol A Terregino, H Liesel Copeland, Sonia Garcia Laumbach, Daniel Mehan, Dana Dunleavy, Thomas Geiger
BACKGROUND: Can a locally developed multiple mini interview (MMI) process lead to outcomes reflective of local values and mission? METHODS: In 2017, the authors performed a retrospective analysis of the relationship of MMI with multiple-choice-based outcomes and non-multiple-choice-based outcomes, including clerkship competencies, OSCE, scholarship/service/leadership, academic honor society induction, peer and faculty humanism nominations, and overall performance at graduation for two entering classes with acceptance decisions based exclusively on a locally developed MMI...
February 19, 2018: Medical Teacher
https://www.readbyqxmd.com/read/29457847/preconditioning-with-low-level-laser-irradiation-enhances-the-therapeutic-potential-of-human-adipose-derived-stem-cells-in-a-mouse-model-of-photoaged-skin
#10
Xuan Liao, Sheng-Hong Li, Guang-Hui Xie, Shan Xie, Li-Ling Xiao, Jian-Xing Song, Hong-Wei Liu
The present study was conducted to explore the therapeutic potential of human adipose-derived stem cells (ADSCs) irradiated with a low level laser (LLL). Cultured ADSCs were treated with 650-nm GaAlAs laser irradiation at 2, 4, and 8 J/cm2 . Cell proliferation was quantified by MTT assays, cytokine secretion was determined by enzyme-linked immunosorbent assays, and adipogenic differentiation was examined by oil red O staining. Additionally, the expression profiles of putative ADSC surface markers were analyzed by quantitative real-time PCR...
February 19, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29457837/improved-wound-remodeling-correlates-with-modulated-tgf-beta-expression-in-skin-diabetic-wounds-following-combined-red-and-infrared-photobiomodulation-treatments
#11
Reza Fekrazad, Arash Sarrafzadeh, Katayoun Am Kalhori, Imran Khan, Praveen R Arany, Alessio Giubellino
Diabetic wounds are a major cause of morbidity among patients with poorly controlled blood glucose levels. Conventional empirical wound care strategies have shown limited efficacy and there is an urgent need to develop novel therapeutic strategies. Photobiomodulation treatments have shown a positive therapeutic effects in several cell culture and animal models. In this study, we examined wound healing in diabetic rats following treatments with two laser wavelengths, namely red (660nm) and infrared (808nm) individually and in combination as compared to routine wound dressings...
February 19, 2018: Photochemistry and Photobiology
https://www.readbyqxmd.com/read/29457659/improved-production-purification-and-characterization-of-biosurfactants-produced-by-serratia-marcescens-sm3-and-its-isogenic-smrg-5-strain
#12
Nashbly Sarela Rosas-Galván, Fernando Martínez-Morales, Silvia Marquina-Bahena, Raunel Tinoco-Valencia, Leobardo Serrano-Carreón, Brandt Bertrand, Renato León-Rodríguez, Josefina Guzmán-Aparicio, Laura Alvaréz-Berber, María Del Refugio Trejo-Hernández
In this study the biosurfactant (Bs) production of two Serratia marcescens strains (SM3 and its isogenic SMRG-5 strain) was improved and the tenso-active agents were purified and characterized. A 23 factorial design was used to evaluate the effect of nitrogen and carbon sources on the surface tension (ST) reduction and emulsion index (EI24 ) of the produced Bs. Optimum Bs production by SM3 was achieved at high concentrations of carbon and nitrogen, reducing ST to 26.5 ± 0.28 dynes/cm, with an EI24 of 79.9 ± 0...
February 19, 2018: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/29457648/reduction-of-infection-risk-meditated-by-co-culturing-vibrio-parahaemolyticus-and-listeria-monocytogenes-in-refrigerated-cooked-shrimp
#13
Ben Niu, Lili Mu, Lili Xiao, Zhaohuan Zhang, Pradeep K Malakar, Haiquan Liu, Yingjie Pan, Yong Zhao
BACKGRROUD: Vibrio parahaemolyticus and Listeria monocytogenes are seafood pathogens of public health significance and predictive models are effective tools for quantitative microbial risk assessment (QMRA) of these pathogens. However, most current predictive models are based on growth of single strains in broth cultures and interactions of two or more bacteria in a food matrix can skew the outcomes of the predictions. Therefore, the impact of V. parahaemolyticus and L. monocytogenes when co-cultured and in mono culture on cooked shrimp at cold storage was investigated in this study...
February 19, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29457630/combination-drug-delivery-via-multilamellar-vesicles-enables-targeting-of-tumor-cells-and-tumor-vasculature
#14
Yarong Liu, Yu Jeong Kim, Natnaree Siriwon, Jennifer A Rohrs, Zhiqiang Yu, Pin Wang
Blood vessel development is critical for the continued growth and progression of solid tumors and, therefore, makes an attractive target for improving cancer therapy. Indeed, vascular-targeted therapies have been extensively explored but they have shown minimal efficacy as monotherapies. Combretastatin A4 (CA-4) is a tubulin-binding vascular disrupting agent that selectively targets the established tumor endothelium, causing rapid vascular beak down. Despite its potent anticancer potential, the drug has dose-limiting side effects, particularly in the form of cardiovascular toxicity...
February 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29457597/injectable-collagen-rgd-systems-for-bone-tissue-engineering-applications
#15
Fu-Chen Kung
Imbalance crosslink density and polymer concentration gradient is formed within the traditional alginate hydrogel using calcium chloride as a crosslinking agent in external gelation for instantaneously process. In this studying, type I collagen (Col I) blended calcium salt form of poly(γ-glutamic acid) (γCaPGA) was mixing with RGD-modified alginate with convenient gelation process and suitable for practical use. The hydrophilicity of the resulting hydrogels was evaluated through swelling tests, water retention capacity tests, and water vapor permeation tests...
2018: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/29457591/effects-of-polycaprolactone-biphasic-calcium-phosphate-scaffolds-on-enhancing-growth-and-differentiation-of-osteoblasts
#16
Nuttawut Thuaksuban, Naruporn Monmaturapoj, Thunmaruk Luntheng
BACKGROUND: Polycaprolactone (PCL)-biphasic calcium phosphate (BCP) scaffolds fabricated using Melt-Stretching and Compression Molding (MSCM) can release calcium and phosphate ions, which are essential for bone formation. OBJECTIVE: Responses of the osteoblasts seeded on three groups of scaffolds including group A; PCL-20% BCP (%wt), group B; PCL-30% BCP and group C (control); pure PCL (100% PCL) were evaluated. METHODS: The cell-scaffold constructs were made by seeding osteoblast cell lines at 1×105 cells/scaffold...
2018: Bio-medical Materials and Engineering
https://www.readbyqxmd.com/read/29457534/comparing-hydrogels-for-human-nucleus-pulposus-cell-regeneration-role-of-osmolarity-during-expansion
#17
Anita Krouwels, Ferry Melchels, Mattie H P Van Rijen, F Cumhur Oner, Wouter Dhert, M Tryfonidou, Laura Creemers
Hydrogels can facilitate nucleus pulposus regeneration, either for clinical application or research into mechanisms of regeneration. However, many different hydrogels and culture conditions for human degenerated nucleus pulposus (NP) have been employed, making literature data difficult to compare. Therefore, we compared six different hydrogels of natural polymers and investigated the role of serum in the medium and of osmolarity during expansion or redifferentation in an attempt to provide comparators for future studies...
February 17, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29457402/-clinical-study-on-negative-pressure-closed-drainage-combined-with-vancomycin-loaded-calcium-sulfate-and-autogenous-bone-in-the-treatment-of-chronic-osteomyelitis
#18
Qiang Li, Shi-Feng Song, Wei Zhang, Guo-Zhi Wu, Li-Zhu Liu
OBJECTIVE: To study the clinical effects of negative pressure closed drainage combined with vancomycin loaded calcium sulfate and autogenous bone in the treatment of chronic osteomyelitis. METHODS: From June 2013 to December 2016, there were 35 cases of chronic osteomyelitis patients in our department, including 23 males and 12 females, ranging in age from 11 to 65 years old, with an average of 34 years old. The course of disease ranged from 8 to 46 months, with an average of 26 months...
November 25, 2017: Zhongguo Gu Shang, China Journal of Orthopaedics and Traumatology
https://www.readbyqxmd.com/read/29457303/long-term-effects-of-art-what-do-animals-tell-us
#19
REVIEW
V Duranthon, P Chavatte-Palmer
Early stages of mammalian embryonic development are now known to be very sensitive to their microenvironment, with long term effects on fetal, postnatal and adult health, thus extending to these early stages the concept of Developmental Origin of Health and Disease (DoHaD). In this scientific context, and with 3% of births in developed countries, safety of Assisted Reproductive Techniques procedures becomes a matter of concern. Besides, embryo technologies in domestic mammals, using huge number of embryos, do not seem to evidence heavy impacts on adult phenotypes...
February 19, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29457125/enhanced-differentiation-of-dental-pulp-cells-cultured-on-microtubular-polymer-scaffolds-in-vitro
#20
Morteza Haeri, Karen Sagomonyants, Mina Mina, Liisa T Kuhn, A Jon Goldberg
Dental caries (tooth decay) is the most common chronic disease. Dental tissue engineering is a promising alternative approach to alleviate the shortcomings of the currently available restorative materials. Mimicking the natural extracellular matrix (ECM) could enhance the performance of tissue engineering scaffolds. In this study, we developed microtubular (~20 μm diameter) polymethyl methacrylate (PMMA) scaffolds resembling the tubular (~2.5 μm diameter) structure of dentin, the collagen-based mineralized tissue that forms the major portion of teeth, to study the effect of scaffold architecture on differentiation of mouse dental pulp cells in vitro ...
June 2017: Regenerative Engineering and Translational Medicine
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