keyword
https://read.qxmd.com/read/38523237/fucosyltransferase-8-regulates-adult-neurogenesis-and-cognition-of-mice-by-modulating-the-itga6-pi3k-akt-signaling-pathway
#21
JOURNAL ARTICLE
Hongfeng Guo, Qihang Sun, Xiaoli Huang, Xiaohao Wang, Feng Zhang, Wenzheng Qu, Jinling Liu, Xuejun Cheng, Qiang Zhu, Wen Yi, Qiang Shu, Xuekun Li
Fucosyltransferase 8 (Fut8) and core fucosylation play critical roles in regulating various biological processes, including immune response, signal transduction, proteasomal degradation, and energy metabolism. However, the function and underlying mechanism of Fut8 and core fucosylation in regulating adult neurogenesis remains unknown. We have shown that Fut8 and core fucosylation display dynamic features during the differentiation of adult neural stem/progenitor cells (aNSPCs) and postnatal brain development...
March 22, 2024: Science China. Life Sciences
https://read.qxmd.com/read/38522823/the-lps-inactivating-enzyme-acyloxyacyl-hydrolase-protects-the-brain-from-experimental-stroke
#22
JOURNAL ARTICLE
Yuanbo Zhu, Yue Hu, Zhongwang Liu, Luping Chang, Xue Geng, Xuhui Yin, Bing-Qiao Zhao, Wenying Fan
Blood-brain-barrier (BBB) disruption is a pathological hallmark of ischemic stroke, and inflammation occurring at the BBB contributes to the pathogenesis of ischemic brain injury. Lipopolysaccharide (LPS), a cell wall component of Gram-negative bacteria, is elevated in patients with acute stroke. The activity of LPS is controlled by acyloxyacyl hydrolase (AOAH), a host enzyme that deacylates LPS to inactivated forms. However, whether AOAH influences the pathogenesis of ischemic stroke remain elusive. We performed in vivo experiments to explore the role and mechanism of AOAH on neutrophil extravasation, BBB disruption, and brain infarction...
March 22, 2024: Translational Research: the Journal of Laboratory and Clinical Medicine
https://read.qxmd.com/read/38522759/the-adam17-inhibitor-zldi-8-sensitized-hepatocellular-carcinoma-cells-to-sorafenib-through-notch1-integrin-%C3%AE-talk
#23
JOURNAL ARTICLE
Chang Xu, Xudong Gao, Tianshu Ren, Hefeng Geng, Kaisi Yang, Yaoguang Huang, Weige Zhang, Shanbo Hou, Aigang Song, Yingshi Zhang, Qingchun Zhao
ZLDI-8 is an A disintegrin and metalloproteinase domain 17 (ADAM17) inhibitor that suppresses the shedding of Notch1 to the Notch1 intracellular domain (NICD). In previous studies, we found that ZLDI-8 was able to sensitize HCC to sorafenib, but the mechanism of action remains unclear. The sensitizing effects of ZLDI-8 were tested both in vitro and in vivo. EMT-related factors, sorafenib sensitivity-related proteins and ECM-related gene expression were assessed using immunohistochemistry, RTPCR and Western blotting...
March 22, 2024: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://read.qxmd.com/read/38522633/generation-of-induced-pluripotent-stem-cells-from-rat-fibroblasts-and-optimization-of-its-differentiation-into-mature-functional-neurons
#24
JOURNAL ARTICLE
Ram Kuwar, Ning Zhang, Adam McQuiston, Xuejun Wen, Dong Sun
BACKGROUND: Induced pluripotent stem cells (iPSCs) derived neural stem cells (NSCs) provide a potential for autologous neural transplantation therapy following neurological insults. Thus far, in preclinical studies the donor iPSCs-NSCs are mostly of human or mouse origin with concerns centering around graft rejection when applied to rat brain injury models. For better survival and integration of transplanted cells in the injured brain in rat models, use of rat-iPSC-NSCs and in combination with biomaterials is of advantageous...
March 22, 2024: Journal of Neuroscience Methods
https://read.qxmd.com/read/38521303/biomimetic-design-of-fibril-forming-non-immunogenic-collagen-like-proteins-for-tissue-engineering
#25
JOURNAL ARTICLE
Mayilvahanan Aarthy, Thiagarajan Hemalatha, Pandurangan Suryalakshmi, Vetrivel Vinoth, Jebakumar Mercyjayapriya, Ganesh Shanmugam, Niraikulam Ayyadurai
Collagen, a key component of extracellular matrix serves as a linchpin for maintaining structural integrity and functional resilience. Concerns over purity and immunogenicity of animal-derived collagens have spurred efforts to develop synthetic collagen-based biomaterials. Despite several collagen mimics, there remains limited exploration of non-immunogenic biomaterials with the capacity for effective self-assembly. To combat the lacuna, collagen like protein (CLP) variants were rationally designed and recombinantly expressed, incorporating human telopeptide sequences (CLP-N and CLP-NC) and bioactive binding sites (CLP-NB)...
March 21, 2024: International Journal of Biological Macromolecules
https://read.qxmd.com/read/38521069/nanodrug-bacteria-conjugates-mediated-oncogenic-collagen-depletion-enhances-immune-checkpoint-blockade-therapy-against-pancreatic-cancer
#26
JOURNAL ARTICLE
Zhaoting Li, Fanyi Mo, Kai Guo, Shuai Ren, Yixin Wang, Yu Chen, Patrick B Schwartz, Nathaniel Richmond, Fengyuan Liu, Sean M Ronnekleiv-Kelly, Quanyin Hu
BACKGROUND: Pancreatic ductal adenocarcinoma (PDAC) cancer cells specifically produce abnormal oncogenic collagen to bind with integrin α3β1 receptor and activate the downstream focal adhesion kinase (FAK), protein kinase B (AKT), and mitogen-activated protein kinase (MAPK) signaling pathway. Collectively, this promotes immunosuppression and tumor proliferation and restricts the response rate of clinical cancer immunotherapies. METHODS: Here, by leveraging the hypoxia tropism and excellent motility of the probiotic Escherichia coli strain Nissle 1917 (ECN), we developed nanodrug-bacteria conjugates to penetrate the extracellular matrix (ECM) and shuttle the surface-conjugated protein cages composed of collagenases and anti-programmed death-ligand 1 (PD-L1) antibodies to PDAC tumor parenchyma...
March 21, 2024: Med
https://read.qxmd.com/read/38520952/inhibition-of-platelet-adhesion-to-exposed-subendothelial-collagen-by-steric-hindrance-with-blocking-peptide-nanoparticles
#27
JOURNAL ARTICLE
Anqi Wang, Kai Yue, Xiaotong Yan, Weishen Zhong, Genpei Zhang, Lei Wang, Hua Zhang, Xinxin Zhang
The inhibition of platelet adhesion to collagen in exposed vessels represents an innovative approach to the treatment of atherosclerosis and thrombosis. This study aimed to engineer peptide-based nanoparticles that prevent platelet binding to subendothelial collagen by engaging with collagen with high affinity. We examined the interactions between integrin α2/ glycoprotein VI/ von Willebrand factor A3 domain and collagen, as well as between the synthesized peptide nanoparticles and collagen, utilizing molecular dynamics simulations and empirical assays...
March 20, 2024: Colloids and Surfaces. B, Biointerfaces
https://read.qxmd.com/read/38519394/integrin-%C3%AE-v%C3%AE-6-targeted-peptide-toxin-therapy-in-a-novel-%C3%AE-v%C3%AE-6-expressing-immunocompetent-model-of-pancreatic-cancer
#28
JOURNAL ARTICLE
Nicholas F Brown, Elizabeth R Murray, Lauren C Cutmore, Philip Howard, Luke Masterson, Francesca Zammarchi, John A Hartley, Patrick H van Berkel, John F Marshall
Previously we reported that a novel αvβ6-specific peptide-drug conjugate (SG3299) could eliminate established human pancreatic ductal adenocarcinoma (PDAC) xenografts. However the development of effective therapies for PDAC, which is an essential need, must show efficacy in relevant immunocompetent animals. Previously we reported that the KPC mouse transgenic PDAC model that closely recapitulates most stages of development of human PDAC, unlike in humans, failed to express αvβ6 on their tumours or metastases...
February 24, 2024: Pancreatology: Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]
https://read.qxmd.com/read/38518897/recent-advances-in-vascular-thiol-isomerases-and-redox-systems-in-platelet-function-and-thrombosis
#29
REVIEW
David W Essex, Lu Wang
There have been substantial advances in vascular protein disulfide isomerases (PDIs) in platelet function and thrombosis in recent years. There are four known prothrombotic thiol isomerases, PDI, ERp57, ERp72, ERp46 and one antithrombotic PDI, transmembrane protein 1 (TMX1). A sixth PDI, ERp5, may exhibit either pro- or anti-thrombotic properties in platelets. Studies on ERp46 in platelet function and thrombosis provide insight into the mechanisms by which these enzymes function. ERp46-catalyzed disulfide cleavage in the αIIbβ3 platelet integrin occurs prior to PDI-catalyzed events to maximally support platelet aggregation...
March 20, 2024: Journal of Thrombosis and Haemostasis: JTH
https://read.qxmd.com/read/38518755/human-seminal-extracellular-vesicles-enhance-endometrial-receptivity-through-leukemia-inhibitory-factor
#30
JOURNAL ARTICLE
Hanshu Wang, Yu Lin, Rongrong Chen, Yu Zhu, Hongqiang Wang, Shengxian Li, Lei Yu, Kaishu Zhang, Yujie Liu, Tao Jing, Fei Sun
Seminal extracellular vesicles (EVs) contain different subgroups which have diverse effects on sperm function. However, the effect of seminal EVs-especially its subgroups, on the endometrial receptivity was largely unknown. Here, we found that the seminal EVs could be divided into high-density EV (EV-H)、medium density EV (EV-M) and low-density EV (EV-L), after purification using iodixanol. Then we demonstrated that EV-H could promote the expression and secretion of leukemia inhibitor factor (LIF) in human endometrial cells...
March 22, 2024: Endocrinology
https://read.qxmd.com/read/38517345/peptidoglycan-from-bacillus-anthracis-inhibits-human-macrophage-efferocytosis-in-part-by-reducing-cell-surface-expression-of-mertk-and-tim-3
#31
JOURNAL ARTICLE
Joshua S Mytych, Zijian Pan, Charmaine Lopez-Davis, Nancy Redinger, Christina Lawrence, Jadith Ziegler, Narcis I Popescu, Judith A James, A Darise Farris
Bacillus anthracis peptidoglycan (PGN) is a major component of the bacterial cell wall and a key pathogen-associated molecular pattern contributing to anthrax pathology, including organ dysfunction and coagulopathy. Increases in apoptotic leukocytes are a late-stage feature of anthrax and sepsis, suggesting there is a defect in apoptotic clearance. In this study, we tested the hypothesis that B. anthracis PGN inhibits the capacity of human monocyte-derived macrophages (MΦ) to efferocytose apoptotic cells...
March 1, 2024: ImmunoHorizons
https://read.qxmd.com/read/38515944/milk-fat-globule-epidermal-growth-factor-8-alleviates-liver-injury-in-severe-acute-pancreatitis-by-restoring-autophagy-flux-and-inhibiting-ferroptosis-in-hepatocytes
#32
JOURNAL ARTICLE
Qing Cui, Hang-Cheng Liu, Wu-Ming Liu, Feng Ma, Yi Lv, Jian-Cang Ma, Rong-Qian Wu, Yi-Fan Ren
BACKGROUND: Liver injury is common in severe acute pancreatitis (SAP). Excessive autophagy often leads to an imbalance of homeostasis in hepatocytes, which induces lipid peroxidation and mitochondrial iron deposition and ultimately leads to ferroptosis. Our previous study found that milk fat globule epidermal growth factor 8 (MFG-E8) alleviates acinar cell damage during SAP via binding to αvβ3/5 integrins. MFG-E8 also seems to mitigate pancreatic fibrosis via inhibiting chaperone-mediated autophagy...
February 21, 2024: World Journal of Gastroenterology: WJG
https://read.qxmd.com/read/38514757/paper-based-electrochemical-device-for-early-detection-of-integrin-%C3%AE-v%C3%AE-6-expressing-tumors
#33
JOURNAL ARTICLE
Stefano Cinti, Stefano Tomassi, Chiara Ciardiello, Rossella Migliorino, Marinella Pirozzi, Alessandra Leone, Elena Di Gennaro, Virginia Campani, Giuseppe De Rosa, Vincenzo Maria D'Amore, Salvatore Di Maro, Greta Donati, Sima Singh, Ada Raucci, Francesco Saverio Di Leva, Horst Kessler, Alfredo Budillon, Luciana Marinelli
Despite progress in the prevention and diagnosis of cancer, current technologies for tumor detection present several limitations including invasiveness, toxicity, inaccuracy, lengthy testing duration and high cost. Therefore, innovative diagnostic techniques that integrate knowledge from biology, oncology, medicinal and analytical chemistry are now quickly emerging in the attempt to address these issues. Following this approach, here we developed a paper-based electrochemical device for detecting cancer-derived Small Extracellular Vesicles (S-EVs) in fluids...
March 21, 2024: Communications Chemistry
https://read.qxmd.com/read/38514598/revealing-a-novel-methylated-integrin-alpha-8-related-to-extracellular-matrix-and-anoikis-resistance-using-proteomic-analysis-in-the-immune-microenvironment-of-lung-adenocarcinoma
#34
JOURNAL ARTICLE
Tingting Liu, Wen Ji, Xue Cheng, Lin Lv, Xiaohui Yu, Na Wang, Mengcong Li, Tinghua Hu, Zhihong Shi
Genomic epigenetics of extracellular matrix (ECM) play an important role in lung adenocarcinoma (LUAD). Our study identified a signature of potential prognostic genes associated with ECM and constructed immune risk-related prognosis model in LUAD. We downloaded mRNAs transcriptome data, miRNAs expression data, and clinical patient information for LUAD based on The Cancer Genome Atlas. "Limma, clusterProfiler, ggplot2" R packages and GSEA were used to analyze meaningful genes and explore potential biological function...
March 21, 2024: Molecular Biotechnology
https://read.qxmd.com/read/38514304/unlocking-mechanosensitivity-integrins-in-neural-adaptation
#35
REVIEW
Fanny Jaudon, Lorenzo A Cingolani
Mechanosensitivity extends beyond sensory cells to encompass most neurons in the brain. Here, we explore recent research on the role of integrins, a diverse family of adhesion molecules, as crucial biomechanical sensors translating mechanical forces into biochemical and electrical signals in the brain. The varied biomechanical properties of neuronal integrins, including their force-dependent conformational states and ligand interactions, dictate their specific functions. We discuss new findings on how integrins regulate filopodia and dendritic spines, shedding light on their contributions to synaptic plasticity, and explore recent discoveries on how they engage with metabotropic receptors and ion channels, highlighting their direct participation in electromechanical transduction...
March 20, 2024: Trends in Cell Biology
https://read.qxmd.com/read/38513651/mass-spectrometry-analysis-of-glycopeptides-enriched-by-anion-exchange-mediated-methods-reveals-polylacnac-extended-n-glycans-in-integrins-and-tetraspanins-in-melanoma-cells
#36
JOURNAL ARTICLE
Tomislav Čaval, Gege Xu, Maryam Baniasad, Chih-Wei Chu, Rachel Rice, Itati Hundal, Gregg Czerwieniec, Flavio Schwarz
Glycosylation is a key modulator of the functional state of proteins. Recent developments in large-scale analysis of intact glycopeptides have enabled the identification of numerous glycan structures that are relevant in pathophysiological processes. However, one motif found in N -glycans, poly- N -acetyllactosamine (polyLacNAc), still poses a substantial challenge to mass spectrometry-based glycoproteomic analysis due to its relatively low abundance and large size. In this work, we developed approaches for the systematic mapping of polyLacNAc-elongated N -glycans in melanoma cells...
March 21, 2024: Analytical Chemistry
https://read.qxmd.com/read/38513154/bv2-membrane-coated-pegylated-liposomes-delivered-hfgf21-to-cortical-and-hippocampal-microglia-for-alzheimer-s-disease-therapy
#37
JOURNAL ARTICLE
Heng-Cai Wang, Wei Yang, Ling Xu, Yong-Hui Han, Yi Lin, Cui-Tao Lu, Kwonseop Kim, Ying-Zheng Zhao, Xi-Chong Yu
Microglia-mediated inflammation is involved in the pathogenesis of Alzheimer's disease (AD), whereas hFGF21 has demonstrated the ability to regulate microglia activation in Parkinson's disease, indicating a potential therapeutic role in AD. However, challenges such as aggregation, rapid inactivation, and the blood-brain barrier hinder its effectiveness in treating AD. This study develops targeted delivery of hFGF21 to activated microglia using BV2 membrane-coated PEGylated liposomes (hFGF21@BCM-LIP), preserving the bioactivity of hFGF21...
March 21, 2024: Advanced Healthcare Materials
https://read.qxmd.com/read/38513099/the-focal-adhesion-protein-talin-is-a-mechanically-gated-a-kinase-anchoring-protein
#38
JOURNAL ARTICLE
Mingu Kang, Yasumi Otani, Yanyu Guo, Jie Yan, Benjamin T Goult, Alan K Howe
Protein kinase A (PKA) is a ubiquitous, promiscuous kinase whose activity is specified through subcellular localization mediated by A-kinase anchoring proteins (AKAPs). PKA has complex roles as both an effector and a regulator of integrin-mediated cell adhesion to extracellular matrix (ECM). Recent observations demonstrate that PKA is an active component of focal adhesions (FA), suggesting the existence of one or more FA AKAPs. Using a promiscuous biotin ligase fused to PKA type-IIα regulatory (RIIα) subunits and subcellular fractionation, we identify the archetypal FA protein talin1 as an AKAP...
March 26, 2024: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38513010/determination-of-single-molecule-loading-rate-during-mechanotransduction-in-cell-adhesion
#39
JOURNAL ARTICLE
Myung Hyun Jo, Paul Meneses, Olivia Yang, Claudia C Carcamo, Sushil Pangeni, Taekjip Ha
Cells connect with their environment through surface receptors and use physical tension in receptor-ligand bonds for various cellular processes. Single-molecule techniques have revealed bond strength by measuring "rupture force," but it has long been recognized that rupture force is dependent on loading rate-how quickly force is ramped up. Thus, the physiological loading rate needs to be measured to reveal the mechanical strength of individual bonds in their functional context. We have developed an overstretching tension sensor (OTS) to allow more accurate force measurement in physiological conditions with single-molecule detection sensitivity even in mechanically active regions...
March 22, 2024: Science
https://read.qxmd.com/read/38511888/cd93-regulates-breast-cancer-growth-and-vasculogenic-mimicry-through-the-pi3k-akt-sp2-signaling-pathway-activated-by-integrin-%C3%AE-1
#40
JOURNAL ARTICLE
Hong Liu, Jianhui Zhang, Yanjun Zhao, Zhixiong Fan, Yongheng Yang, Yuanyuan Mao, Jingyuan Yang, Shungao Ma
In women, breast cancer (BC) accounts for 7%-10% of all cancer cases and is one of the most common cancers. To identify a new method for treating BC, the role of CD93 and its underlying mechanism were explored. MDA-MB-231 cells were used in this study and transfected with si-CD93, si-MMRN2, oe-CD93, si-integrin β1, or oe-SP2 lentivirus. After MDA-MB-231 cells were transfected with si-NC or si-CD93, they were injected into nude mice by subcutaneous injection at a dose of 5 × 106 /mouse to construct a BC animal model...
April 2024: Journal of Biochemical and Molecular Toxicology
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