Read by QxMD icon Read

Protein AND weight loss

Sander A J Verbrugge, Martin Schönfelder, Lore Becker, Fakhreddin Yaghoob Nezhad, Martin Hrabě de Angelis, Henning Wackerhage
Skeletal muscle mass differs greatly in mice and humans and this is partially inherited. To identify muscle hypertrophy candidate genes we conducted a systematic review to identify genes whose experimental loss or gain-of-function results in significant skeletal muscle hypertrophy in mice. We found 47 genes that meet our search criteria and cause muscle hypertrophy after gene manipulation. They are from high to small effect size: Ski, Fst, Acvr2b, Akt1, Mstn, Klf10, Rheb, Igf1, Pappa, Ppard, Ikbkb, Fstl3, Atgr1a, Ucn3, Mcu, Junb, Ncor1, Gprasp1, Grb10, Mmp9, Dgkz, Ppargc1a (specifically the Ppargc1a4 isoform), Smad4, Ltbp4, Bmpr1a, Crtc2, Xiap, Dgat1, Thra, Adrb2, Asb15, Cast, Eif2b5, Bdkrb2, Tpt1, Nr3c1, Nr4a1, Gnas, Pld1, Crym, Camkk1, Yap1, Inhba, Tp53inp2, Inhbb, Nol3, Esr1 ...
2018: Frontiers in Physiology
Debashis Dutta, Mira Debnath DAS
 In present study, Taguchi's design of experiment L9 orthogonal array was created using Qualitek-4 software with four most critical factors namely, K2 HPO4 , MgSO4 , CaCl2 and culture pH. Production of a new intracellular antifungal protein in submerged fermentation was optimized with yield of 0.98±0.1 mg/gram dry cell weight mycelia from Aspergillus giganteus MTCC 8408. The average molecular mass of the purified protein was figured as 5.122 kDa using Electro Spray Ionization-Mass Spectrometry. Scanning electron microscopy was used to correlate the effect of selected factors on fungal cell morphology and its metabolite production...
2018: Biocontrol Science
Cintia Scucuglia Heluany, Leonard de Vinci Kanda Kupa, Mariana Nascimento Viana, Cristina Maria Fernandes, Sandra Helena Poliselli Farsky
The genesis of rheumatoid arthritis (RA) is complex and dependent on genetic background and exposure to environmental xenobiotic. Indeed, smoking is associated to developing and worsening pre-existing RA. Nevertheless, the mechanisms and cigarette compounds involved in the harmful processes have not been elucidated. Here, we investigated if the exposure to hydroquinone (HQ), an abundant pro-oxidative compound of cigarette and benzene metabolite, could worsen the ongoing RA. Hence, collagen-induced arthritis (CIA) was induced in male Wistar rats by s...
June 14, 2018: Chemico-biological Interactions
Andrew G Diamos, Hugh S Mason
Recombinant virus-like particles (VLPs) are proven to be safe and effective vaccine candidates. We have previously described a plant-based recombinant protein expression system based on agroinfiltration of a replicating vector derived from the geminivirus bean yellow dwarf virus (BeYDV). The system has been systematically optimized to improve expression and reduce cell death in Nicotiana benthamiana leaves. Using these modifications, we show that VLPs derived from genotype GII.4 norovirus, the leading cause of acute gastroenteritis worldwide, can be produced at >1 mg/g leaf fresh weight (LFW), over three times the highest level ever reported in plant-based systems...
June 14, 2018: Protein Expression and Purification
Maria Grazia Zizzo, Gaetano Caldara, Annalisa Bellanca, Domenico Nuzzo, Marta Di Carlo, Rosa Serio
BACKGROUND: Guanosine, a guanine-based purine, is an extracellular signaling molecule exerting anti-inflammatory and antioxidative effects in several in vivo and in vitro injury models. We aimed to investigate its protective effects on 2, 4-dinitrobenzene sulfonic acid (DNBS)-induced colitis in rat. METHODS: Rats were divided into five groups and colitis was induced by intracolonic instillation of DNBS (15 mg/rat). Guanosine (4 or 8 mg/kg) was administered for 6 days i...
June 15, 2018: Inflammopharmacology
Nerylee Ann Watson, Kathryn Ann Dyer, Jonathan David Buckley, Grant David Brinkworth, Alison Mary Coates, Gaynor Parfitt, Peter Ranald Charles Howe, Manny Noakes, Karen Joy Murphy
BACKGROUND: Although higher-protein diets (HP) can assist with weight loss and glycemic control, their effect on psychological wellbeing has not been established. The objective of this study was to compare the effects of a HP and a higher-carbohydrate diet (HC), combined with regular exercise, on psychological wellbeing both during weight loss (WL) and weight maintenance phases (WM). METHODS: In a parallel RCT, 61 adults with T2D (mean ± SD: BMI 34.3 ± 5...
June 15, 2018: Nutrition Journal
Qi Deng, Yaling Wang, Lijun Sun, Jianrong Li, Zhijia Fang, Ravi Gooneratne
Water and protein are major constituents of shrimp, any changes in protein and the state of water influence the quality of shrimp. Therefore, a study to examine the law of moisture migration and protein denaturation under different freezing and thawing conditions is important. The proton density images of thawed frozen-shrimp revealed that the water loss during quick-freezing was much greater than that during slow freezing or microfreezing. At room temperature (25 °C), the water loss from brine-thawing was more than still-water thawing and still-water thawing was more than thawing spontaneously...
June 15, 2018: Journal of Food Science
Nienke M van Loon, Roelof Ottenhoff, Sander Kooijman, Martina Moeton, Saskia Scheij, Reinout L P Roscam Abbing, Marion J J Gijbels, Johannes H M Levels, Vincenzo Sorrentino, Jimmy F P Berbée, Patrick C N Rensen, Noam Zelcer
OBJECTIVE: The E3 ubiquitin ligase IDOL (inducible degrader of the LDLR [LDL (low-density lipoprotein) receptor]) is a post-transcriptional regulator of LDLR abundance. Model systems and human genetics support a role for IDOL in regulating circulating LDL levels. Whether IDOL plays a broader metabolic role and affects development of metabolic syndrome-associated comorbidities is unknown. APPROACH AND RESULTS: We studied WT (wild type) and Idol ( -/ - ) (Idol-KO) mice in 2 models: physiological aging and diet-induced obesity...
June 14, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
Valarie A Pallatto, Molly A Bechtold
A 9-year-old spayed female English Mastiff was referred for outpatient ultrasound due to a 3-week history of weight loss, vomiting, and decreased appetite. Abdominal ultrasound showed multiple splenic masses of varying sizes and serum chemistry panel showed hyperglobulinemia. Cytologic examination of fine-needle aspirates of the splenic masses indicated a mast cell and plasma cell collision tumor. Results of serum and urine protein electrophoresis and immunofixation indicated the plasma cell neoplasia was producing IgA immunoglobulins...
June 2018: Veterinary Clinical Pathology
J Uerlings, Z G Song, X Y Hu, S K Wang, H Lin, Johan Buyse, N Everaert
Dysfunction of the intestinal epithelial barrier under elevated temperatures is assumed to prompt pathological conditions and to eventually impede chickens' growth, resulting in massive economic losses in broiler industries. The aims of this research were to determine the impact of acute heat stress on the intestinal tight junction network of broiler chicks (Gallus domesticus L.) and to elucidate whether adenosine monophosphate-activated protein kinase (AMPK) was involved in the integrated response of the broiler's gastrointestinal tract to heat stress...
June 13, 2018: Poultry Science
Ting Yu, Qian Yu, Xiaotian Chen, Lixing Zhou, Yuming Wang, Chenggong Yu
Although enteral nutrition therapy for inflammatory bowel disease has been confirmed to be an effective treatment method, the exact mechanism responsible for the effects of enteral nutrition remains unclear. The aim of the present study was to investigate the protective effect of exclusive enteral nutrition (EEN) against colitis, and to elucidate the potential mechanisms by inhibiting p65 activation via regulating the p38/mitogen‑ and stress‑activated protein kinase‑1 (MSK1) pathway. Experiments were performed by establishing dextran sulfate sodium (DSS)‑mice colitis and picrylsulfonic acid solution (TNBS)‑induced rat colitis, and the results demonstrated that EEN treatment attenuated body weight loss, colon length shortening and colonic pathological damage caused by colitis...
June 4, 2018: International Journal of Molecular Medicine
Saranya Sridhar, Alexander Luedtke, Edith Langevin, Ming Zhu, Matthew Bonaparte, Tifany Machabert, Stephen Savarino, Betzana Zambrano, Annick Moureau, Alena Khromava, Zoe Moodie, Ted Westling, Cesar Mascareñas, Carina Frago, Margarita Cortés, Danaya Chansinghakul, Fernando Noriega, Alain Bouckenooghe, Josh Chen, Su-Peing Ng, Peter B Gilbert, Sanjay Gurunathan, Carlos A DiazGranados
Background In efficacy trials of a tetravalent dengue vaccine (CYD-TDV), excess hospitalizations for dengue were observed among vaccine recipients 2 to 5 years of age. Precise risk estimates according to observed dengue serostatus could not be ascertained because of the limited numbers of samples collected at baseline. We developed a dengue anti-nonstructural protein 1 (NS1) IgG enzyme-linked immunosorbent assay and used samples from month 13 to infer serostatus for a post hoc analysis of safety and efficacy...
June 13, 2018: New England Journal of Medicine
Elisa Banchi, Fabio Candotto Carniel, Alice Montagner, Francesco Petruzzellis, Gregor Pichler, Valentino Giarola, Dorothea Bartels, Alberto Pallavicini, Mauro Tretiach
The relation between water status and expression profiles of desiccation -related genes has been studied in the desiccation tolerant (DT) aeroterrestrial green microalga Trebouxia gelatinosa, a common lichen photobiont. Algal colonies were desiccated in controlled conditions and during desiccation water content (WC) and water potential (Ψ) were measured to find the turgor loss point (Ψtlp ). Quantitative real-time PCR was performed to measure the expression of ten genes related to photosynthesis, antioxidant defense, expansins, heat shock proteins (HSPs), and desiccation related proteins in algal colonies collected during desiccation when still at full turgor (WC > 6 g H2 O g-1 dry weight), immediately before and after Ψtlp (-4 MPa; WC ∼ 1 g H2 O g-1 dry weight) and before and after complete desiccation (WC < 0...
June 5, 2018: Plant Physiology and Biochemistry: PPB
Takeshi Nishino, Takahiro Yoshida, Masakazu Goto, Seiya Inoue, Takuya Minato, Satoshi Fujiwara, Yota Yamamoto, Yoshihito Furukita, Yasuhiro Yuasa, Hiromichi Yamai, Hirokazu Takechi, Hiroaki Toba, Hiromitsu Takizawa, Mitsuteru Yoshida, Junichi Seike, Takanori Miyoshi, Akira Tangoku
BACKGROUND: Daikenchuto (TJ-100), a traditional Japanese herbal medicine, is widely used in Japan. Its effects on gastrointestinal motility and microcirculation and its anti-inflammatory effect are known. The purpose of this prospective randomized controlled trial was to investigate the effect of TJ-100 after esophagectomy in esophageal cancer patients. METHODS: Forty patients for whom subtotal esophageal resection for esophageal cancer was planned at our institute from March 2011 to August 2013 were enrolled and divided into two groups at the point of determination of the operation schedule after informed consent was obtained: a TJ-100 (15 g/day)-treated group (n = 20) and a control group (n = 20)...
April 2018: Esophagus: Official Journal of the Japan Esophageal Society
E Vlassi, P Vlachos, M Kornaros
Table grapes artificially inoculated with B. Cinerea were tested under four different ozonation strategies in order to achieve prolongation of table grapes' shelf-life time. Decay incidence, external disease appearance, number of infected grapes, weight loss and a variety of quality parameters such as sugars and proteins content were checked after every 3 days. No significant alteration of table grapes quality characteristics was observed after their exposure to ozone atmosphere. Moreover, the low ozone dosage process (0...
June 2018: Journal of Food Science and Technology
Jian Li, Fen Wang, Chunli Ma, Yuchen Huang, Dian Wang, Dexing Ma
Avian coccidiosis leads to severe economic losses for the global poultry industry. Apical membrane antigen 1 (AMA1) of E. tenella (EtAMA1) plays a vital role during invasion of parasites into host cells. In the present study, recombinant live Lactococcus lactis expressing cytoplasmic, secreted and cell wall-anchored EtAMA1 protein were respectively constructed. The three live bacteria were respectively administered orally to SPF chickens (100 μl bacteria containing 5 × 109  CFU per chicken) for three times at 10-day intervals...
May 17, 2018: Experimental Parasitology
Hui Qiao, Fengwei Jiang, Yiwei Xiong, Sufei Jiang, Hongtuo Fu, Fei Li, Wenyi Zhang, Shengming Sun, Shubo Jin, Yongsheng Gong, Yan Wu
The oriental river prawn, Macrobrachium nipponense, is an important commercial aquaculture resource in China. In order to overwinter, M. nipponense displays decreased physiological activity and less consumption of energy. Sudden warming would trigger molting and cause an extensive death, resulting in huge economic losses. Therefore, it is of great practical significance to study the molting mechanism of oriental river prawns. Molt-inhibiting hormone gene (MIH) plays a major role in regulating molting in crustaceans...
2018: PloS One
Giti Kalantarian, Nasrin Ziamajidi, Reza Mahjoub, Mohammad Taghi Goodarzi, Massoud Saidijam, Sara Soleimani Asl, Roghayeh Abbasalipourkabir
BACKGROUND: Subcutaneous injection of insulin can lead to problems such as hypoglycemia and edema. OBJECTIVE: The purpose of this research was to evaluate the effect of oral insulin-coated trimethyl chitosan nanoparticles on control of glycemic status, IGF-1 and IGF-2 levels, and apoptosis in the hippocampus of rats with diabetes mellitus. METHODS: Insulin-coated trimethyl chitosan nanoparticles were prepared by the complex polyelectrolyte (PEC) method...
June 6, 2018: Restorative Neurology and Neuroscience
Inga Zerr, Piero Parchi
Sporadic Creutzfeldt-Jakob disease (CJD), the most common human prion disease, is generally regarded as a spontaneous neurodegenerative illness, arising either from a spontaneous PRNP somatic mutation or a stochastic PrP structural change. Alternatively, the possibility of an infection from animals or other source remains to be completely ruled out. Sporadic CJD is clinically characterized by rapidly progressive dementia with ataxia, myoclonus, or other neurologic signs and, neuropathologically, by the presence of aggregates of abnormal prion protein, spongiform change, neuronal loss, and gliosis...
2018: Handbook of Clinical Neurology
Susanna Klevebro, Vera Westin, Elisabeth Stoltz Sjöström, Mikael Norman, Magnus Domellöf, Anna-Karin Edstedt Bonamy, Boubou Hallberg
BACKGROUND & AIM: Extremely preterm infants face substantial neonatal morbidity. Nutrition is important to promote optimal growth and organ development in order to reduce late neonatal complications. The aim of this study was to examine the associations of early nutritional intakes on growth and risks of bronchopulmonary dysplasia (BPD) and retinopathy of prematurity (ROP) in a high-risk population. METHODS: This population-based cohort study includes infants born before 27 0/7 weeks of gestational age without severe malformations and surviving ≥10 days...
May 29, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"