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https://www.readbyqxmd.com/read/29429342/modular-engineering-intracellular-nadh-regeneration-boosts-extracellular-electron-transfer-of-shewanella-oneidensis-mr-1
#1
Feng Li, Yuanxiu Li, Li-Ming Sun, Xiaoli Chen, Xingjuan An, Changji Yin, Yingxiu Cao, Hui Wu, Hao Song
Efficient extracellular electron transfer (EET) of exoelectrogens is essentially for practical applications of versatile bio-electrochemical systems. Intracellular electrons flow from NADH to extracellular electron accepters via EET pathways. However, it was yet established how the manipulation of intracellular NADH impacted the EET efficiency. Strengthening NADH regeneration from NAD+, as a feasible approach for cofactor engineering, has been used in regulating the intracellular NADH pool and the redox state (NADH/NAD+ ratio) of cells...
February 11, 2018: ACS Synthetic Biology
https://www.readbyqxmd.com/read/29411858/photo-switchable-microbial-fuel-cells
#2
Orr Schlesinger, Rambabu Dandela, Ashok Bhagat, Michael M Meijler, Lin Xia, Lital Alfonta
Regulation of Biosystems in a clean, simple and efficient way is important for the design of smart bio-interfaces and bioelecronic devices. Light as a non-invasive mean to control the activity of a protein enables spatial and temporal control far superior to other chemical and physical methods. The ability to regulate the activity of a catalytic enzyme in a biofuel-cell reduces the waste of resources and energy and turns the fuel-cell into a smart and more efficient device for power generation. Here we present a microbial-fuel-cell based on a surface displayed, photo-switchable alcohol dehydrogenase...
February 7, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29382103/green-synthesis-of-three-dimensional-hybrid-n-doped-orr-electro-catalysts-derived-from-apricot-sap
#3
Ramesh Karunagaran, Campbell Coghlan, Cameron Shearer, Diana Tran, Karan Gulati, Tran Thanh Tung, Christian Doonan, Dusan Losic
Rapid depletion of fossil fuel and increased energy demand has initiated a need for an alternative energy source to cater for the growing energy demand. Fuel cells are an enabling technology for the conversion of sustainable energy carriers (e.g., renewable hydrogen or bio-gas) into electrical power and heat. However, the hazardous raw materials and complicated experimental procedures used to produce electro-catalysts for the oxygen reduction reaction (ORR) in fuel cells has been a concern for the effective implementation of these catalysts...
January 28, 2018: Materials
https://www.readbyqxmd.com/read/29371883/conversion-of-lignocellulosic-agave-residues-into-liquid-biofuels-using-an-afex%C3%A2-based-biorefinery
#4
Carlos A Flores-Gómez, Eleazar M Escamilla Silva, Cheng Zhong, Bruce E Dale, Leonardo da Costa Sousa, Venkatesh Balan
Background: Agave-based alcoholic beverage companies generate thousands of tons of solid residues per year in Mexico. These agave residues might be used for biofuel production due to their abundance and favorable sustainability characteristics. In this work, agave leaf and bagasse residues from species Agave tequilana and Agave salmiana were subjected to pretreatment using the ammonia fiber expansion (AFEX) process. The pretreatment conditions were optimized using a response surface design methodology...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29358378/redirection-of-lipid-flux-toward-phospholipids-in-yeast-increases-fatty-acid-turnover-and-secretion
#5
Raphael Ferreira, Paulo Gonçalves Teixeira, Verena Siewers, Jens Nielsen
Bio-based production of fatty acids and fatty acid-derived products can enable sustainable substitution of petroleum-derived fuels and chemicals. However, developing new microbial cell factories for producing high levels of fatty acids requires extensive engineering of lipid metabolism, a complex and tightly regulated metabolic network. Here we generated a Saccharomyces cerevisiae platform strain with a simplified lipid metabolism network with high-level production of free fatty acids (FFAs) due to redirected fatty acid metabolism and reduced feedback regulation...
January 22, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29287734/air-bio-battery-with-a-gas-liquid-porous-diaphragm-cell-for-medical-and-health-care-devices
#6
Takahiro Arakawa, Rui Xie, Fumiya Seshima, Koji Toma, Kohji Mitsubayashi
Powering future generations of medical and health care devices mandates the transcutaneous transfer of energy or harvesting energy from the human body fluid. Glucose-driven bio fuel cells (bio-batteries) demonstrate promise as they produce electrical energy from glucose, which is a substrate presents in physiological fluids. Enzymatic biofuel cells can convert chemical energy into electrical energy using enzymes as catalysts. In this study, an air bio-battery was developed for healthcare and medical applications, consisting of a glucose-driven enzymatic biofuel cell using a direct gas-permeable membrane or a gas/liquid porous diaphragm...
December 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29276820/water-oxygen-circulation-system-based-fuel-free-bio-photoelectrochemical-cells-by-integrating-a-ni-feooh-bivo4-photoanode
#7
He Zhang, You Yu, Lingling Zhang, Shaojun Dong
In this study, a self-circulation "water-oxygen-water" system-based fuel-free bio-photoelectrochemical cell (BPEC) was fabricated, which consists of Ni:FeOOH modified n-type bismuth vanadate (BiVO4) photoanode and laccase catalyzed biocathode. In this BPEC, irradiation of the photoanode generates photocurrent for photo-oxidation of water to oxygen, which is reduced to water again at laccase biocathode. Noteworthily, the by-products of two electrodes reactions could continue to be reacted, which made the molecules of H2O and O2 be stuck in infinite loop of "water-oxygen-water" without any sacrificial chemical components...
December 24, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29274856/effect-of-biochar-on-bio-electrochemical-dye-degradation-and-energy-production
#8
Carmalin Sophia Ayyappan, V M Bhalambaal, Sunil Kumar
The effect of coconut shell biochar on dye degradation in a microbial fuel cell (MFC) was investigated in the present study. Two different doses of biochar (0.5 g and 1 g) and one control without bio-char were studied. The highest COD removal efficiency was about 77.7% (0.5 g biochar), maximum current (1.07 mA) and voltage (722 mV) were obtained with 1 g biochar. Biofilm optical microscopy characterization revealed the micro colonies intricate plate-like structures. High adsorbent dosage might provide a high surface area for biofilm to generate electricity...
December 16, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29236006/electricity-generation-through-a-photo-sediment-microbial-fuel-cell-using-algae-at-the-cathode
#9
B Neethu, M M Ghangrekar
Sediment microbial fuel cells (SMFCs) are bio-electrochemical devices generating electricity from redox gradients occurring across the sediment-water interface. Sediment microbial carbon-capture cell (SMCC), a modified SMFC, uses algae grown in the overlying water of sediment and is considered as a promising system for power generation along with algal cultivation. In this study, the performance of SMCC and SMFC was evaluated in terms of power generation, dissolved oxygen variations, sediment organic matter removal and algal growth...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29224103/sex-differences-and-role-of-estradiol-in-hypoglycemia-associated-counter-regulation
#10
Karen P Briski, Hussain N Alhamami, Ayed Alshamrani, Santosh K Mandal, Manita Shakya, Mostafa H H Ibrahim
Vital nerve cell functions, including maintenance of transmembrane voltage and information transfer, occur at high energy expense. Inadequate provision of the obligate metabolic fuel glucose exposes neurons to risk of dysfunction or injury. Clinical hypoglycemia rarely occurs in nondiabetic individuals but is an unfortunate regular occurrence in patients with type 1 or advanced insulin-treated type 2 diabetes mellitus. Requisite strict glycemic control, involving treatment with insulin, sulfonylureas, or glinides, can cause frequent episodes of iatrogenic hypoglycemia due to defective counter-regulation, including reduced glycemic thresholds and diminished magnitude of motor responses...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/29154092/anoxic-biodegradation-of-triclosan-and-the-removal-of-its-antimicrobial-effect-in-microbial-fuel-cells
#11
Lu Wang, Yulei Liu, Chao Wang, Xiaodan Zhao, Gurumurthy Dummi Mahadeva, Yicheng Wu, Jun Ma, Feng Zhao
Triclosan (TCS) is an emerging organic contaminant in the environment. Here, the anoxic bio-degradation of TCS in microbial fuel cells (MFCs) was explored. It was found that anoxic biodegradation of TCS could be achieved in MFC, and the removal rate of TCS was accelerated after reactor acclimation. After 7 months of operation, 10mg/L TCS could be removed within 8days in MFCs. Fluorescence microscopy results revealed that the microbe cells in the reactors were intact, and the microbes were in active state. Flow cytometry test showed that the proliferation of inoculated microbe was higher in MFC effluent than that in TCS solution...
October 13, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29137930/mitigating-external-and-internal-cathode-fouling-using-a-polymer-bonded-separator-in-microbial-fuel-cells
#12
Wulin Yang, Ruggero Rossi, Yushi Tian, Kyoung-Yeol Kim, Bruce E Logan
Microbial fuel cell (MFC) cathodes rapidly foul when treating domestic wastewater, substantially reducing power production over time. Here a wipe separator was chemically bonded to an activated carbon air cathode using polyvinylidene fluoride (PVDF) to mitigate cathode fouling and extend cathode performance over time. MFCs with separator-bonded cathodes produced a maximum power density of 190 ± 30 mW m(-2) after 2 months of operation using domestic wastewater, which was ∼220% higher than controls (60 ± 50 mW m(-2)) with separators that were not chemically bonded to the cathode...
November 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29112795/bio-analytical-applications-of-microbial-fuel-cell-based-biosensors-for-onsite-water-quality-monitoring
#13
REVIEW
Ahmed ElMekawy, Hanaa M Hegab, Deepak Pant, Christopher P Saint
Globally, sustainable provision of high quality safe water is a major challenge of the 21(st) century. Various chemical and biological monitoring analytics are presently utilized to guarantee the availability of high quality water. However, these techniques still face some challenges including high costs, complex design and onsite and online limitations. The recent technology of utilising microbial fuel cell (MFC)-based biosensors holds outstanding potential for the rapid and real-time monitoring of water source quality...
November 7, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29109396/electricity-generation-from-digitally-printed-cyanobacteria
#14
Marin Sawa, Andrea Fantuzzi, Paolo Bombelli, Christopher J Howe, Klaus Hellgardt, Peter J Nixon
Microbial biophotovoltaic cells exploit the ability of cyanobacteria and microalgae to convert light energy into electrical current using water as the source of electrons. Such bioelectrochemical systems have a clear advantage over more conventional microbial fuel cells which require the input of organic carbon for microbial growth. However, innovative approaches are needed to address scale-up issues associated with the fabrication of the inorganic (electrodes) and biological (microbe) parts of the biophotovoltaic device...
November 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/29101531/investigating-the-underlying-mechanism-of-saccharomyces-cerevisiae-in-response-to-ethanol-stress-employing-rna-seq-analysis
#15
Ruoyun Li, Guotong Xiong, Shukun Yuan, Zufang Wu, Yingjie Miao, Peifang Weng
Saccharomyces cerevisiae has been widely used for wine fermentation and bio-fuels production. A S. cerevisiae strain Sc131 isolated from tropical fruit shows good fermentation properties and ethanol tolerance, exhibiting significant potential in Chinese bayberry wine fermentation. In this study, RNA-sequence and RT-qPCR was used to investigate the transcriptome profile of Sc131 in response to ethanol stress. Scanning Electron Microscopy were carried out to observe surface morphology of yeast cells. Totally, 937 genes were identified differential expressed, including 587 up-regulated and 350 down-regulated genes, after 4-h ethanol stress (10% v/v)...
November 3, 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29089935/design-optimization-and-application-of-small-molecule-biosensor-in-metabolic-engineering
#16
REVIEW
Yang Liu, Ye Liu, Meng Wang
The development of synthetic biology and metabolic engineering has painted a great future for the bio-based economy, including fuels, chemicals, and drugs produced from renewable feedstocks. With the rapid advance of genome-scale modeling, pathway assembling and genome engineering/editing, our ability to design and generate microbial cell factories with various phenotype becomes almost limitless. However, our lack of ability to measure and exert precise control over metabolite concentration related phenotypes becomes a bottleneck in metabolic engineering...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29065460/flotation-assembly-of-large-area-ultrathin-mwcnt-nanofilms-for-construction-of-bioelectrodes
#17
Andrew J Gross, Jules L Hammond, Michael Holzinger, Serge Cosnier
We report a simple, versatile, and rapid method for the fabrication of optically-transparent large-area carbon nanotube (CNT) films via flotation assembly. After solvent-induced assembly, floating films were transferred to a flat supporting substrate to form conductive and transparent CNT film electrodes. The resulting electrodes, with uniform 40 ± 20 nm multi-walled CNT (MWCNT) layers, were characterized by electrochemical and microscopy methods. The flotation method does not require specialized thin-film instrumentation and avoids the need for surfactants and pre-oxidized CNTs which can hamper electrochemical performance...
October 21, 2017: Nanomaterials
https://www.readbyqxmd.com/read/29044893/tsynthetic-klebsiella-pneumoniae-shewanella-oneidensis-consortium-enables-glyc-erol-fed-high-performance-microbial-fuel-cells
#18
Feng Li, Changji Yin, Liming Sun, Yuanxiu Li, Xuewu Guo, Hao Song
Microbial fuel cell (MFC) is an eco-friendly bio-electrochemical system that uses mi-croorganism as biocatalyst to convert biomass into electricity. Glycerol, as a waste in the biodiesel refinery processes, is an appealing substrate for MFC. Nevertheless, glycerol cannot be utilized as carbon source by well-known exoelectrogens such as Shewanella oneidensis. Herein, to generate electricity by rapidly harnessing glycerol, we rationally constructed a Klebsiella pneumoniae-Shewanella oneidensis microbial consortium to efficiently harvest electricity from glycerol, in which K...
October 18, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29038251/stress-introduction-rate-alters-benefit-of-acrab-tolc-efflux-pumps
#19
Ariel M Langevin, Mary J Dunlop
Stress tolerance studies are typically conducted in an all-or-none fashion. However, in realistic settings-such as in clinical or metabolic engineering applications-cells may encounter stresses at different rates. As such, how cells tolerate stress may depend on its rate of appearance. To address this, we study how the rate of introduction affects bacterial stress tolerance by focusing on a key mechanism for stress response. Efflux pumps, such as AcrAB-TolC from Escherichia coli, are membrane transporters well known for their ability to export a wide variety of substrates, including antibiotics, signaling molecules, and biofuels...
October 16, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/29027469/bucking-the-current-trend-in-bioelectrochemical-systems-a-case-for-bioelectroanalytics
#20
Thomas William Seviour, Jamie Hinks
Turning wastewater directly into electricity is alluring, widespread use of microbial fuel cells (MFCs) to achieve this at industrial scale appears increasingly unlikely despite intense research efforts lasting over a decade. Such endeavors have not been futile, however, and game-changing discoveries have resulted from these well-intentioned, scientifically rigorous but ultimately frustrated attempts to resolve the Waste-Energy dichotomy. The appeal of MFCs is largely of conceptual elegance rather than financial competitiveness, based on the green ideal that bacteria can be turned into cost effective bio-batteries...
October 13, 2017: Critical Reviews in Biotechnology
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