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https://www.readbyqxmd.com/read/27900581/molecular-dynamics-simulations-of-the-orientation-properties-of-cytochrome-c-on-the-surface-of-single-walled-carbon-nanotubes
#1
Bing Zhang, Jia Xu, Shu-Fan Mo, Jian-Xi Yao, Song-Yuan Dai
Electron transfer between cytochrome c (Cytc) and electrodes can be influenced greatly by the orientation of protein on the surface of the electrodes. In the present study, different initial orientations of Cytc on the surface of five types of single-walled carbon nanotubes (SWNTs), with different diameters and chirality, were constructed. Properties of the orientations of proteins on the surface of these tubes were first investigated through molecular dynamics simulations. It was shown that variations in SWNT diameter do not significantly affect the orientation; however, the chirality of the SWNTs is crucial to the orientation of the heme embedded in Cytc, and the orientation of the protein can consequently be influenced by the heme orientation...
December 2016: Journal of Molecular Modeling
https://www.readbyqxmd.com/read/27878978/elimination-of-power-overshoot-caused-by-electron-depletion-of-limited-anodic-kinetic-at-bio-anode-through-assistance-current-in-microbial-fuel-cell
#2
Bongkyu Kim, Junyeong An, In Seop Chang
The power overshoot generated by electron depletion in microbial fuel cells (MFCs) was characterized in this study. Various causes of power overshoot, identified in previous studies, are discussed in terms of their plausible contributions to electron depletion. We found that power overshoot occurred if the anodic overpotential generated by electron depletion exceeded the cathodic overpotential. The introduction of assistance current from anode connections, which ameliorated the electron depletion in the MFCs, immediately eliminated the power overshoot...
November 22, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27871379/decolourisation-of-acid-orange-7-in-a-microbial-fuel-cell-with-a-laccase-based-biocathode-influence-of-mitigating-ph-changes-in-the-cathode-chamber
#3
Priyadharshini Mani, Taj Keshavarz, T S Chandra, Godfrey Kyazze
Biocathodes may be a suitable replacement of platinum in microbial fuel cells (MFCs) if the cost of MFCs is to be reduced. However, the use of enzymes as bio-cathodes is fraught with loss of activity as time progresses. A possible cause of this loss in activity might be pH increase in the cathode as pH gradients in MFCs are well known. This pH increase is however, accompanied by simultaneous increase in salinity; therefore salinity may be a confounding variable. This study investigated various ways of mitigating pH changes in the cathode of MFCs and their effect on laccase activity and decolourisation of a model azo dye Acid orange 7 in the anode chamber...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27825877/recent-advances-in-the-development-and-utilization-of-modern-anode-materials-for-high-performance-microbial-fuel-cells
#4
REVIEW
Jayesh M Sonawane, Abhishek Yadav, Prakash C Ghosh, Samuel B Adeloju
Microbial fuel cells (MFCs) are novel bio-electrochemical device for spontaneous or single step conversion of biomass into electricity, based on the use of metabolic activity of bacteria. The design and use of MFCs has attracted considerable interests because of the potential new opportunities they offer for sustainable production of energy from biodegradable and reused waste materials. However, the associated slow microbial kinetics and costly construction materials has limited a much wider commercial use of the technology...
October 5, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27806676/the-role-of-cell-seeding-bio-scaffolds-and-the-in-vivo-microenvironment-in-the-guided-generation-of-osteochondral-composite-tissue
#5
Jiao Wei, Tanja Herrler, Kai Liu, Dong Han, Mei Yang, Chuanchang Dai, Qing Feng Li
Tissue engineering based on cell seeding, bio-scaffolds, and growth factors has been widely applied for the reconstruction of tissue defects. Recent progress has fueled in vivo tissue engineering techniques in becoming hot topics in regenerative medicine and reconstructive surgery. To improve the efficacy of tissue engineering, we here investigated the roles of cell seeding, bio-scaffolds, growth factors, and in-vivo microenvironment in tissue regeneration. Bone marrow derived stem cells (BMSCs), allogenic demineralized bone matrix (DBM) as bio-scaffold, and growth factors bone morphogenetic protein 2/ transforming growth factor (BMP-2/TGF-β1), and the in-vivo microenvironment of rib periosteum and perichondrium were used in different combinations for the generation of osteochondral composite tissue...
November 2, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27761130/production-strategies-and-applications-of-microbial-single-cell-oils
#6
Katrin Ochsenreither, Claudia Glück, Timo Stressler, Lutz Fischer, Christoph Syldatk
Polyunsaturated fatty acids (PUFAs) of the ω-3 and ω-6 class (e.g., α-linolenic acid, linoleic acid) are essential for maintaining biofunctions in mammalians like humans. Due to the fact that humans cannot synthesize these essential fatty acids, they must be taken up from different food sources. Classical sources for these fatty acids are porcine liver and fish oil. However, microbial lipids or single cell oils, produced by oleaginous microorganisms such as algae, fungi and bacteria, are a promising source as well...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27746307/metabolically-engineered-saccharomyces-cerevisiae-for-branched-chain-ester-productions
#7
Jifeng Yuan, Pranjul Mishra, Chi Bun Ching
Medium branched-chain esters can be used not only as a biofuel but are also useful chemicals with various industrial applications. The development of economically feasible and environment friendly bio-based fuels requires efficient cell factories capable of producing desired products in high yield. Herein, we sought to use a number of strategies to engineer Saccharomyces cerevisiae for high-level production of branched-chain esters. Mitochondrion-based expression of ATF1 gene in a base strain with an overexpressed valine biosynthetic pathway together with expression of mitochondrion-relocalized α-ketoacid decarboxylase (encoded by ARO10) and alcohol dehydrogenase (encoded by ADH7) not only produced isobutyl acetate, but also 3-methyl-1-butyl acetate and 2-methyl-1-butyl acetate...
October 13, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27736793/exploring-hydrogenotrophic-methanogenesis-a-genome-scale-metabolic-reconstruction-of-methanococcus-maripaludis
#8
Matthew A Richards, Thomas J Lie, Juan Zhang, Stephen W Ragsdale, John A Leigh, Nathan D Price
: Hydrogenotrophic methanogenesis occurs in multiple environments ranging from the intestinal tracts of animals to anaerobic sediments and hot springs. Energy conservation in hydrogenotrophic methanogens was long a mystery; only within the last decade, it was reported that net energy conservation for growth depends on electron bifurcation. In this work we focus on Methanococcus maripaludis, a well-studied hydrogenotrophic marine methanogen. To better understand hydrogenotrophic methanogenesis and compare it with methylotrophic methanogenesis that utilizes oxidative phosphorylation rather than electron bifurcation, we have built iMR539, a genome scale metabolic reconstruction that accounts for 539 of the 1722 protein-coding genes of M...
October 10, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27687501/co2-fixation-by-anaerobic-non-photosynthetic-mixotrophy-for-improved-carbon-conversion
#9
Shawn W Jones, Alan G Fast, Ellinor D Carlson, Carrissa A Wiedel, Jennifer Au, Maciek R Antoniewicz, Eleftherios T Papoutsakis, Bryan P Tracy
Maximizing the conversion of biogenic carbon feedstocks into chemicals and fuels is essential for fermentation processes as feedstock costs and processing is commonly the greatest operating expense. Unfortunately, for most fermentations, over one-third of sugar carbon is lost to CO2 due to the decarboxylation of pyruvate to acetyl-CoA and limitations in the reducing power of the bio-feedstock. Here we show that anaerobic, non-photosynthetic mixotrophy, defined as the concurrent utilization of organic (for example, sugars) and inorganic (for example, CO2) substrates in a single organism, can overcome these constraints to increase product yields and reduce overall CO2 emissions...
2016: Nature Communications
https://www.readbyqxmd.com/read/27684325/laboratory-production-of-biofuels-and-biochemicals-from-a-rapeseed-oil-through-catalytic-cracking-conversion
#10
Siauw H Ng, Yu Shi, Nicole E Heshka, Yi Zhang, Edward Little
The work is based on a reported study which investigates the processability of canola oil (bio-feed) in the presence of bitumen-derived heavy gas oil (HGO) for production of transportation fuels through a fluid catalytic cracking (FCC) route. Cracking experiments are performed with a fully automated reaction unit at a fixed weight hourly space velocity (WHSV) of 8 hr(-1), 490-530 °C, and catalyst/oil ratios of 4-12 g/g. When a feed is in contact with catalyst in the fluid-bed reactor, cracking takes place generating gaseous, liquid, and solid products...
2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27681904/aspergillus-oryzae-saccharomyces-cerevisiae-consortium-allows-bio-hybrid-fuel-cell-to-run-on-complex-carbohydrates
#11
Justin P Jahnke, Thomas Hoyt, Hannah M LeFors, James J Sumner, David M Mackie
Consortia of Aspergillus oryzae and Saccharomyces cerevisiae are examined for their abilities to turn complex carbohydrates into ethanol. To understand the interactions between microorganisms in consortia, Fourier-transform infrared spectroscopy is used to follow the concentrations of various metabolites such as sugars (e.g., glucose, maltose), longer chain carbohydrates, and ethanol to optimize consortia conditions for the production of ethanol. It is shown that with proper design A. oryzae can digest food waste simulants into soluble sugars that S...
February 4, 2016: Microorganisms
https://www.readbyqxmd.com/read/27557613/recent-advances-in-engineering-propionyl-coa-metabolism-for-microbial-production-of-value-added-chemicals-and-biofuels
#12
Kajan Srirangan, Mark Bruder, Lamees Akawi, Dragan Miscevic, Shane Kilpatrick, Murray Moo-Young, C Perry Chou
Diminishing fossil fuel reserves and mounting environmental concerns associated with petrochemical manufacturing practices have generated significant interests in developing whole-cell biocatalytic systems for the production of value-added chemicals and biofuels. Although acetyl-CoA is a common natural biogenic precursor for the biosynthesis of numerous metabolites, propionyl-CoA is unpopular and non-native to most organisms. Nevertheless, with its C3-acyl moiety as a discrete building block, propionyl-CoA can serve as another key biogenic precursor to several biological products of industrial importance...
August 25, 2016: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/27550091/hybrid-bio-photo-electro-chemical-cells-for-solar-water-splitting
#13
Roy I Pinhassi, Dan Kallmann, Gadiel Saper, Hen Dotan, Artyom Linkov, Asaf Kay, Varda Liveanu, Gadi Schuster, Noam Adir, Avner Rothschild
Photoelectrochemical water splitting uses solar power to decompose water to hydrogen and oxygen. Here we show how the photocatalytic activity of thylakoid membranes leads to overall water splitting in a bio-photo-electro-chemical (BPEC) cell via a simple process. Thylakoids extracted from spinach are introduced into a BPEC cell containing buffer solution with ferricyanide. Upon solar-simulated illumination, water oxidation takes place and electrons are shuttled by the ferri/ferrocyanide redox couple from the thylakoids to a transparent electrode serving as the anode, yielding a photocurrent density of 0...
2016: Nature Communications
https://www.readbyqxmd.com/read/27544915/bio-electro-catalytic-treatment-of-petroleum-produced-water-influence-of-cathode-potential-upliftment
#14
Pratiksha Jain, Sandipam Srikanth, Manoj Kumar, Priyangshu M Sarma, M P Singh, Banwari Lal
Treatment of petroleum produced water (PPW) was studied using bioelectrochemical system (BES) under uplifted cathode potential. The treatment efficiency in terms of COD and hydrocarbon removal was observed at 91.25% and 76.60% respectively, along with the reduction in TDS during BES operation under 400mV of cathode potential. There was also a reduction in concentration of sulfates, however, it was not significant at, since oxidative conditions are being maintained at anode. Improved oxidation of PPW at anode also resulted in good power output (-20...
November 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27538988/progesterone-augments-cell-susceptibility-to-hiv-1-and-hiv-1-hsv-2-co-infections
#15
Viswanath Ragupathy, Wang Xue, Ji Tan, Krishnakumar Devadas, Yamei Gao, Indira Hewlett
In human immunodeficiency virus type 1 (HIV-1)-infected women, oral or injectable progesterone containing contraceptive pills may enhance HIV-1 acquisition in vivo, and the mechanism by which this occurs is not fully understood. In developing countries, Herpes simplex virus type-2 (HSV-2) co-infection has been shown to be a risk for increase of HIV-1 acquisition and, if co-infected women use progesterone pills, infections may increase several fold. In this study, we used an in vitro cell culture system to study the effects of progesterone on HIV-1 replication and to explore the molecular mechanism of progesterone effects on infected cells...
October 2016: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/27506964/a-quick-responsive-dna-nanotechnology-device-for-bio-molecular-homeostasis-regulation
#16
Songlin Wu, Pei Wang, Chen Xiao, Zheng Li, Bing Yang, Jieyang Fu, Jing Chen, Neng Wan, Cong Ma, Maoteng Li, Xiangliang Yang, Yi Zhan
Physiological processes such as metabolism, cell apoptosis and immune responses, must be strictly regulated to maintain their homeostasis and achieve their normal physiological functions. The speed with which bio-molecular homeostatic regulation occurs directly determines the ability of an organism to adapt to conditional changes. To produce a quick-responsive regulatory system that can be easily utilized for various types of homeostasis, a device called nano-fingers that facilitates the regulation of physiological processes was constructed using DNA origami nanotechnology...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27502834/biomanufacture-of-nano-pd-0-by-escherichia-coli-and-electrochemical-activity-of-bio-pd-0-made-at-the-expense-of-h2-and-formate-as-electron-donors
#17
J Courtney, K Deplanche, N V Rees, L E Macaskie
OBJECTIVE: Palladised cells of Desulfovibrio desulfuricans and Shewanella oneidensis have been reported as fuel cell electrocatalysts but growth at scale may be unattractive/costly; we have evaluated the potential of using E. coli, using H2/formate for Pd-nanoparticle manufacture. RESULTS: Using 'bio-Pd' made under H2 (20 wt%) cyclic voltammograms suggested electrochemical activity of bio-NPs in a native state, attributed to proton adsorption/desorption. Bio-Pd prepared using formate as the electron donor gave smaller, well separated NPs; this material showed no electrochemical properties, and hence little potential for fuel cell use using a simple preparation technique...
November 2016: Biotechnology Letters
https://www.readbyqxmd.com/read/27501067/nanoscale-electrochemistry-of-sp-2-carbon-materials-from-graphite-and-graphene-to-carbon-nanotubes
#18
Patrick R Unwin, Aleix G Güell, Guohui Zhang
Carbon materials have a long history of use as electrodes in electrochemistry, from (bio)electroanalysis to applications in energy technologies, such as batteries and fuel cells. With the advent of new forms of nanocarbon, particularly, carbon nanotubes and graphene, carbon electrode materials have taken on even greater significance for electrochemical studies, both in their own right and as components and supports in an array of functional composites. With the increasing prominence of carbon nanomaterials in electrochemistry comes a need to critically evaluate the experimental framework from which a microscopic understanding of electrochemical processes is best developed...
September 20, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27469250/the-big-bang-of-genome-editing-technology-development-and-application-of-the-crispr-cas9-system-in-disease-animal-models
#19
Ming Shao, Tian-Rui Xu, Ce-Shi Chen
Targeted genome editing technology has been widely used in biomedical studies. The CRISPR-associated RNA-guided endonuclease Cas9 has become a versatile genome editing tool. The CRISPR/Cas9 system is useful for studying gene function through efficient knock-out, knock-in or chromatin modification of the targeted gene loci in various cell types and organisms. It can be applied in a number of fields, such as genetic breeding, disease treatment and gene functional investigation. In this review, we introduce the most recent developments and applications, the challenges, and future directions of Cas9 in generating disease animal model...
July 18, 2016: Dong Wu Xue Yan Jiu, Zoological Research
https://www.readbyqxmd.com/read/27456909/mixed-cellulose-ester-filter-as-a-separator-for-air-diffusion-cathode-microbial-fuel-cells
#20
Zejie Wang, Bongsu Lim
Separator is important to prevent bio-contamination of the catalyst layer of air-diffusion cathode microbial fuel cells (MFCs). Mixed cellulose ester filter (MCEF) was examined as a separator for an air-cathode MFC in the present study. The MCEF-MFC produced a maximum power density of 780.7±18.7 mW m(-2), which was comparable to 770.9±35.9 mW/m(2) of MFC with Nafion membrane (NFM) as a separator. Long-term examination demonstrated a more stable performance of the MCEF-MFC than NFM-MFC. After 25 cycles, the maximum voltage of the MCEF-MFC decreased by only 1...
July 26, 2016: Environmental Technology
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