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Enzyme hydrogel

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https://www.readbyqxmd.com/read/27924631/cardioprotective-effects-of-curcumin-loaded-magnetic-hydrogel-nanocomposite-nanocurcumin-against-doxorubicin-induced-cardiac-toxicity-in-rat-cardiomyocyte-cell-lines
#1
Mehrdad Namdari, Ali Eatemadi
Curcumin, is a yellow substance extracted from Curcuma longa rhizomes, it is a crystalline compound that has been traditionally applied in culinary practices and medicines in India. The aim of our study is to demonstrate the efficacy of curcumin-loaded magnetic hydrogel nanocomposite in the treatment of heart hypertrophy. 10 rats weighing 150-200 g each were induced with heart failure using 2.5 mg/kg doxorubicin for 2 weeks. The test groups were treated with curcumin-loaded magnetic hydrogel nanocomposite while the control was treated with curcumin alone...
December 7, 2016: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/27919839/heparin-hyaluronic-acid-hydrogel-for-efficient-cellular-activities-of-3d-encapsulated-adipose-derived-stem-cells
#2
Kihak Gwon, Eunsol Kim, Giyoong Tae
We have developed stem cell-responsive, heparin-hyaluronic acid (Hep-HA) hydrogel, crosslinked by thiolated heparin (Hep-SH) and methacrylated hyaluronic acid (HA-MA) via visible light mediated, thiol-ene reaction. Physical properties of the hydrogel (gelation time, storage modulus, and swelling ratio) were tunable by adjusting light intensity, initiator/polymer concentration, and precursor pH. Culture of human adipose derived mesenchymal stem cells (ADSCs) using this hydrogel was characterized and compared with the control hydrogels including Hep-PEG hydrogel, PEG-HA hydrogel...
December 2, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27904965/chitosan-hydrogel-microspheres-an-effective-covalent-matrix-for-crosslinking-of-soluble-dextranase-to-increase-stability-and-recycling-efficiency
#3
Faiza Shahid, Afsheen Aman, Muhammad Asif Nawaz, Asad Karim, Shah Ali Ul Qader
Dextranase is a unique biocatalyst that has high specificity and stereo-selectivity towards a complex biopolymer known as dextran. Dextranase has wide industrial application, but most of the time harsh environmental conditions adversely affect the functionality and stability of the enzyme. To overcome this issue, a covalent cross-linking immobilization method was adapted in the current study utilizing a nontoxic and biocompatible matrix known as chitosan. Chitosan hydrogel microspheres were synthesized using chitosan which exhibited noteworthy physical and mechanical strength...
November 30, 2016: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/27863665/biodegradable-hydrophilic-carriers-for-the-oral-delivery-of-hematological-factor-ix-for-hemophilia-b-treatment
#4
Sarena D Horava, Katie J Moy, Nicholas A Peppas
Current protein replacement therapies for hemophilia B, a genetic bleeding disorder caused by a deficiency in coagulation factor IX, rely on IV injections and infusions. Oral delivery of factor IX is a desirable needle-free option, especially for prophylaxis. We have developed a biodegradable, pH-responsive hydrogel microcarrier system based on the poly(methacrylic acid)-grafted-poly(ethylene glycol) [P(MAA-g-EG)]. Incorporation of an enzymatically degradable peptide crosslinking agent allows for site-specific degradation by trypsin in the small intestine...
November 30, 2016: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/27863177/using-a-redox-modality-to-connect-synthetic-biology-to-electronics-hydrogel-based-chemo-electro-signal-transduction-for-molecular-communication
#5
Yi Liu, Chen-Yu Tsao, Eunkyoung Kim, Tanya Tschirhart, Jessica L Terrell, William E Bentley, Gregory F Payne
A hydrogel-based dual film coating is electrofabricated for transducing bio-relevant chemical information into electronical output. The outer film has a synthetic biology construct that recognizes an external molecular signal and transduces this input into the expression of an enzyme that converts redox-inactive substrate into a redox-active intermediate, which is detected through an amplification mechanism of the inner redox-capacitor film.
November 18, 2016: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/27819009/chitin-and-chitosan-production-and-application-of-versatile-biomedical-nanomaterials
#6
Daniel Elieh-Ali-Komi, Michael R Hamblin
Chitin is the most abundant aminopolysaccharide polymer occurring in nature, and is the building material that gives strength to the exoskeletons of crustaceans, insects, and the cell walls of fungi. Through enzymatic or chemical deacetylation, chitin can be converted to its most well-known derivative, chitosan. The main natural sources of chitin are shrimp and crab shells, which are an abundant byproduct of the food-processing industry, that provides large quantities of this biopolymer to be used in biomedical applications...
March 2016: International Journal of Advanced Research
https://www.readbyqxmd.com/read/27808567/evaluation-of-superabsorbent-linseed-polysaccharides-as-a-novel-stimuli-responsive-oral-sustained-release-drug-delivery-system
#7
Muhammad Tahir Haseeb, Muhammad Ajaz Hussain, Sajid Bashir, Muhammad Umer Ashraf, Naveed Ahmad
CONTEXT: Advancement in technology has transformed the conventional dosage forms to intelligent drug delivery systems. Such systems are helpful for targeted and efficient drug delivery with minimum side effects. Drug release from these systems are governed and controlled by external stimuli (pH, enzymes, ions, glucose, etc.). Polymeric biomaterial having stimuli responsive properties has opened a new area in drug delivery approach. OBJECTIVE: Potential of a polysaccharide (rhamnogalacturonan) based hydrogel from Linseeds (Linum usitatissimum L...
November 3, 2016: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/27804288/protein-cross-linked-hydrogels-with-tailored-swelling-and-bioactivity-performance-a-comparative-study
#8
Bin Li, Kaixuan Ren, Yupeng Wang, Yanxin Qi, Xuesi Chen, Yubin Huang
The design of protein-based hydrogels that include biological activity independent of structural functionality is desirable for many bioengineering applications. Here a general route for construction of protein-based hydrogel is proposed by pre-treatment of protein with thiolation agent and succeeding conjugation with 4-arm PEG-acrylate via Michael addition reactions. Different swelling behaviors responding to temperature and ions are comparatively studied for hydrogel cross-linked with hemoglobin (multimeric protein), albumin (monomeric protein) and dithiothreitol (DTT, small molecule)...
November 2, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27797504/ligand-receptor-interaction-modulates-the-energy-landscape-of-enzyme-instructed-self-assembly-of-small-molecules
#9
Richard Haburcak, Junfeng Shi, Xuewen Du, Dan Yuan, Bing Xu
The concurrence of enzymatic reaction and ligand-receptor interactions is common for proteins, but rare for small molecules and has yet to be explored. Here we show that ligand-receptor interaction modulates the morphology of molecular assemblies formed by enzyme-instructed assembly of small molecules. While the absence of ligand-receptor interaction allows enzymatic dephosphorylation of a precursor to generate the hydrogelator that self-assembles to form long nanofibers, the presence of the ligand-receptor interaction biases the pathway to form precipitous aggregates containing short nanofibers...
October 31, 2016: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/27792001/angiogenic-potential-of-endothelial-and-tumor-cells-seeded-on-gelatin-based-hydrogels-in-response-to-electrical-stimulations
#10
Rumiana Tzoneva, Veselina Uzunova, Sonia Apostolova, Anne Krüger-Genge, Axel T Neffe, Friedrich Jung, Andreas Lendlein
Angiogenesis is one of the key processes during development, wound healing and tumor formation. Prerequisite for its existence is the presence of endogenous electrical fields (EFs) generated by active ion transport across polarized epithelia and endothelia, and appearance of the transcellular potentials. During angiogenesis cellular factor as endothelial growth factor (VEGF), synthesis of adhesive proteins and membrane metalloproteinases (MMPs) govern the angiogenic response to different external stimuli as biomaterials interactions and/or exogenous EF...
October 27, 2016: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/27789882/kinetic-characterization-thermo-stability-and-reactive-red-195a-dye-detoxifying-properties-of-manganese-peroxidase-coupled-gelatin-hydrogel
#11
Muhammad Bilal, Muhammad Asgher, Hongbo Hu, Xuehong Zhang
An indigenous and industrially important manganese peroxidase (MnP) was isolated from solid-state bio-processing of wheat bran by white-rot fungal strain Ganoderma lucidum IBL-05 under pre-optimized growth conditions. Crude MnP extract was partially purified (2.34-fold) to apparent homogeneity by ammonium sulphate precipitation and dialysis. The homogeneous enzyme preparation was encapsulated on gelatin matrix using glutaraldehyde as a cross-linking agent. Optimal conditions for highest immobilization (82.5%) were: gelatin 20% (w/v), glutaraldehyde 0...
October 2016: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/27789373/design-of-hydrogels-of-5-hydroxymethyl-tolterodine-and-their-studies-on-pharmacokinetics-pharmacodynamics-and-transdermal-mechanism
#12
Wenhua Liu, Lirong Teng, Kongtong Yu, Xiangshi Sun, Chunyu Fan, Chaoxing Long, Na Liu, Shuang Li, Bing Wu, Qingji Xu, Fengying Sun, Youxin Li
development, single-factor experiments were employed to evaluate the effect of adding different matrix, enhancers, 5-HMT, ethanol and glycerol on drug skin development, single-factor experiments were employed to evaluate the effect of adding different matrix, enhancers, 5-HMT, ethanol and glycerol on drug skin permeation. Finally, Carbopol 940 was selected as the gel matrix with N-methyl pyrrolidone (NMP) chosen as the enhancer. The relationship between time and the steady accumulative percutaneous amount (Q, μgcm(-2)) of optimized 5-HMT hydrogels was Q4-12h=1290...
October 24, 2016: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/27785356/recent-advances-in-covalent-site-specific-protein-immobilization
#13
REVIEW
Morten Meldal, Sanne Schoffelen
The properties of biosensors, biomedical implants, and other materials based on immobilized proteins greatly depend on the method employed to couple the protein molecules to their solid support. Covalent, site-specific immobilization strategies are robust and can provide the level of control that is desired in this kind of application. Recent advances include the use of enzymes, such as sortase A, to couple proteins in a site-specific manner to materials such as microbeads, glass, and hydrogels. Also, self-labeling tags such as the SNAP-tag can be employed...
2016: F1000Research
https://www.readbyqxmd.com/read/27775890/injectable-polypeptide-hydrogels-with-tunable-microenvironment-for-3d-spreading-and-chondrogenic-differentiation-of-bone-marrow-derived-mesenchymal-stem-cells
#14
Kaixuan Ren, Haitao Cui, Qinghua Xu, Chaoliang He, Gao Li, Xuesi Chen
Bone marrow-derived mesenchymal stem cells (BMSCs) possess vast potential for tissue engineering and regenerative medicine. In this study, an injectable hydrogel comprising poly(L-glutamic acid)-graft-tyramine (PLG-g-TA) with tunable microenvironment was developed via enzyme-catalyzed crosslinking, and used as artificial extracellular matrix (ECM) to explore the behaviors of BMSCs during three dimensional (3D) culture. It was found that the mechanical property, porous structure as well as degradation process of the hydrogels could be tuned by changing the copolymer concentration...
October 24, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27770422/ferrocene-modified-linear-poly-ethylenimine-for-enzymatic-immobilization-and-electron-mediation
#15
David P Hickey
Enzymatic glucose biosensors and biofuel cells make use of the electrochemical transduction between an oxidoreductase enzyme, such as glucose oxidase (GOx), and an electrode to either quantify the amount of glucose in a solution or generate electrical energy. However, many enzymes including GOx are not able to electrochemically interact with an electrode surface directly, but require an external electrochemical relay to shuttle electrons to the electrode. Ferrocene-modified linear poly(ethylenimine) (Fc-LPEI) redox polymers have been designed to simultaneously immobilize glucose oxidase (GOx) at an electrode and mediate electron transfer from their flavin adenine dinucleotide (FAD) active site to the electrode surface...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27769941/enzyme-mediated-stiffening-hydrogels-for-probing-activation-of-pancreatic-stellate-cells
#16
Hung-Yi Liu, Tanja Greene, Tsai-Yu Lin, Camron S Dawes, Murray Korc, Chien-Chi Lin
: The complex network of biochemical and biophysical cues in the pancreatic desmoplasia not only presents challenges to the fundamental understanding of tumor progression, but also hinders the development of therapeutic strategies against pancreatic cancer. Residing in the desmoplasia, pancreatic stellate cells (PSCs) are the major stromal cells affecting the growth and metastasis of pancreatic cancer cells by means of paracrine effects and extracellular matrix protein deposition. PSCs remain in a quiescent/dormant state until they are 'activated' by various environmental cues...
October 18, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27762537/biomimetic-hydrogel-composites-for-soil-stabilization-and-contaminant-mitigation
#17
Zhi Zhao, Nasser Hamdan, Li Shen, Hanqing Nan, Abdullah Almajed, Edward Kavazanjian, Ximin He
We have developed a novel method to synthesize a hyper-branched biomimetic hydrogel network across a soil matrix to improve the mechanical strength of the loose soil and simultaneously mitigate potential contamination due to excessive ammonium. This method successfully yielded a hierarchical structure that possesses the water retention, ion absorption, and soil aggregation capabilities of plant root systems in a chemically controllable manner. Inspired by the robust organic-inorganic composites found in many living organisms, we have combined this hydrogel network with a calcite biomineralization process to stabilize soil...
November 3, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27760397/a-thermo-degradable-hydrogel-with-light-tunable-degradation-and-drug-release
#18
Jingjing Hu, Yihua Chen, Yunqi Li, Zhengjie Zhou, Yiyun Cheng
The development of thermo-degradable hydrogels is of great importance in drug delivery. However, it still remains a huge challenge to prepare thermo-degradable hydrogels with inherent degradation, reproducible, repeated and tunable dosing. Here, we reported a thermo-degradable hydrogel that is rapidly degraded above 44 °C by a facile chemistry. Besides thermo-degradability, the hydrogel also undergoes rapid photolysis with ultraviolet light. By embedding photothermal nanoparticles or upconversion nanoparticles into the gel, it can release the entrapped cargoes such as dyes, enzymes and anticancer drugs in an on-demand and dose-tunable fashion upon near-infrared light exposure...
October 11, 2016: Biomaterials
https://www.readbyqxmd.com/read/27758025/generation-of-large-scale-dna-hydrogels-with-excellent-blood-and-cell-compatibility
#19
Heidi Stoll, Heidrun Steinle, Katharina Stang, Silju Kunnakattu, Lutz Scheideler, Bernd Neumann, Julia Kurz, Ilka Degenkolbe, Nadja Perle, Christian Schlensak, Hans Peter Wendel, Meltem Avci-Adali
Hemocompatibility and cytocompatibility of biomaterials codetermine the success of tissue engineering applications. DNA, the natural component of our cells, is an auspicious biomaterial for the generation of designable scaffolds with tailorable characteristics. In this study, a combination of rolling circle amplification and multiprimed chain amplification is used to generate hydrogels at centimeter scale consisting solely of DNA. Using an in vitro rotation model and fresh human blood, the reaction of the hemostatic system on DNA hydrogels is analyzed...
October 19, 2016: Macromolecular Bioscience
https://www.readbyqxmd.com/read/27753168/stimuli-sensitive-injectable-hydrogels-based-on-polysaccharides-and-their-biomedical-applications
#20
Thavasyappan Thambi, V H Giang Phan, Doo Sung Lee
Stimuli-sensitive injectable polymeric hydrogels are one of the promising delivery vehicles for the controlled release of bioactive agents. In aqueous solutions, these polymers are able to switch sol-to-gel transitions in response to various stimuli including pH, temperature, light, enzyme and magnetic field. Therapeutic agents, including chemotherapeutic agents, protein drugs or cells, are easily mixed with the low-viscous polymer solution at room temperature. Therapeutic-agents-containing solutions are readily injected into target sites through syringe or catheter, which could form hydrogel depot and serve as bioactive molecules release carriers...
December 2016: Macromolecular Rapid Communications
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