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Enzyme hydrogel

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https://www.readbyqxmd.com/read/28429928/a-dual-enzyme-hydrogen-peroxide-generation-machinery-in-hydrogels-supports-antimicrobial-wound-treatment
#1
Daniela Huber, Gregor Tegl, Anna Mensah, Bianca Beer, Martina Baumann, Nicole Borth, Christoph Sygmund, Roland Ludwig, Georg M Guebitz
The aging population and accompanying diseases like diabetes resulted in an increased occurrence of chronic wounds. Topical wound treatment with antimicrobial agents to inhibit bacterial invasion and promote wound healing is often associated with difficulties. Here, we investigated the potential of succinyl chitosan (SC) - carboxymethyl cellulose (CMC) hydrogels which constantly release clinically relevant levels of hydrogen peroxide (H2O2). CMC hydrogel matrix was in-situ converted by limited hydrolysis by a cellulase into substrates accepted by cellobiose dehydrogenase (CDH) for continuous production of H2O2 (30 µM over 24 h)...
April 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28421746/multifunctional-enzymatically-generated-hydrogels-for-chronic-wound-application
#2
Ivaylo Stefanov, Sílvia Pérez-Rafael, Javier Hoyo, Jonathan Cailloux, Orlando O Santana Perez, Dolores Hinojosa-Caballero, Tzanko Tzanov
The healing of chronic wounds requires intensive medical intervention at huge healthcare costs. Dressing materials should consider the multifactorial nature of these wounds comprising deleterious proteolytic and oxidative enzymes, and high bacterial load. In this work, multifunctional hydrogels for chronic wound application were produced by enzymatic crosslinking of thiolated chitosan and gallic acid. The hydrogels combine several beneficial to wound healing properties, controlling the matrix metalloproteinases (MMPs) and myeloperoxidase (MPO) activities, oxidative stress and bacterial contamination...
April 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28412223/bioengineered-robust-hybrid-hydrogels-enrich-the-stability-and-efficacy-of-biological-drugs
#3
Moon Soo Gil, Jinhwan Cho, Thavasyappan Thambi, V H Giang Phan, Inchan Kwon, Doo Sung Lee
Biological drugs are exquisitely tailored components offering the advantages of high specificity and efficacy that are considered safe for treating diseases. Nevertheless, the effectiveness of biological drugs is limited by their inherent short biological half-life and poor stability in vivo. Herein, we engineered a novel delivery platform based on hybrid injectable hydrogels, in which pH- and temperature-responsive biodegradable copolymers were site-specifically coupled to the sulfhydryl group of human serum albumin, which effectively enhances the stability and circulation half-life of the biological drug, recombinant uricase enzyme (Uox)...
April 12, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28411636/armored-urease-enzyme-bioconjugated-poly-acrylamide-hydrogel-as-a-storage-and-sensing-platform
#4
Konda R Kunduru, S N Raju Kutcherlapati, Dhamodaran Arunbabu, Tushar Jana
Jack bean urease is an important enzyme not only because of its numerous uses in medical and other fields but also because of its historical significance-the first enzyme to be crystallized and also the first nickel metalloenzyme. This enzyme hydrolyzes urea into ammonia and carbon dioxide; however, the stability of this enzyme at ambient temperature is a bottleneck for its applicability. To improve urease stability, it was immobilized on different substrates, particularly on polymeric hydrogels. In this study, the enzyme was coupled covalently with poly(acrylamide) hydrogel with an yield of 18μmol/cm(3)...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28408827/photothermal-modulated-drug-delivery-and-magnetic-relaxation-based-on-collagen-poly-%C3%AE-glutamic-acid-hydrogel
#5
Sun-Hee Cho, Ahreum Kim, Woojung Shin, Min Beom Heo, Hyun Jong Noh, Kwan Soo Hong, Jee-Hyun Cho, Yong Taik Lim
Injectable and stimuli-responsive hydrogels have attracted attention in molecular imaging and drug delivery because encapsulated diagnostic or therapeutic components in the hydrogel can be used to image or change the microenvironment of the injection site by controlling various stimuli such as enzymes, temperature, pH, and photonic energy. In this study, we developed a novel injectable and photoresponsive composite hydrogel composed of anticancer drugs, imaging contrast agents, bio-derived collagen, and multifaceted anionic polypeptide, poly (γ-glutamic acid) (γ-PGA)...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28407335/in-vitroporcine-blastocyst-development-in-three-dimensional-alginate-hydrogels
#6
REVIEW
Jeremy R Miles, Taylor D Laughlin, Catherine N Sargus-Patino, Angela K Pannier
Appropriate embryonic and fetal development significantly impact pregnancy success and, therefore, the efficiency of swine production. The pre-implantation period of porcine pregnancy is characterized by several developmental hallmarks, which are initiated by the dramatic morphological change that occurs as pig blastocysts elongate from spherical to filamentous blastocysts. Deficiencies in blastocyst elongation contribute to approximately 20% of embryonic loss, and have a direct influence on within-litter birth weight variation...
April 13, 2017: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/28406045/a-methacrylic-hyaluronic-acid-derivative-for-potential-application-in-oral-treatment-of-celiac-disease
#7
Giovanna Pitarresi, Fabio Salvatore Palumbo, Daniela Triolo, Calogero Fiorica, Gaetano Giammona
OBJECTIVE: Aim of this work was the synthesis of a methacrylic hyaluronic acid derivative and the production, via photocrosslinking, of related hydrogels loaded with an endopeptidase intended for a potential oral treatment of celiac disease. METHODS: The methacrylic derivative of hyaluronic acid (HA) was prepared through a one-pot procedure involving the reaction with ethylenediamine (EDA) and methacrylic anhydride (MA). The obtained derivative, named HA-EDA-MA, was used to prepare photocrosslinked hydrogels loaded with a prolyl endopeptidase derived from Flavobacterium meningosepticum (PEP FM) able to detoxify gliadin...
April 13, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28406043/cardioprotective-effect-of-magnetic-hydrogel-nanocomposite-loaded-n-%C3%AE-l-rhamnopyranosyl-vincosamide-isolated-from-moringa-oleifera-leaves-against-doxorubicin-induced-cardiac-toxicity-in-rats-in-vitro-and-in-vivo-studies
#8
Mostafa Cheraghi, Mehrdad Namdari, Hadis Daraee, Babak Negahdari, Hammed T Aiyelabegan
Cardioprotective effect of N, α-L-rhamnopyranosyl vincosamide (VR), isolated from the leaves of Moringa oleifera plant in doxorubicin-induced cardiac toxicity rats was evaluated. Twelve (12) rats were randomly selected into 3 groups; 2 rats received distilled water in the control group, 5 rats in group I received varying concentration of VR treatment, and group II containing 5 rats received varying concentration of VR-loaded magnetic hydrogel nanocomposite. Malondialdehyde (MDA), glutathione peroxidase (GSH) and superoxide dismutase (SOD) enzymes activities level were analysed after two weeks...
April 13, 2017: Journal of Microencapsulation
https://www.readbyqxmd.com/read/28405647/enzyme-induced-dual-network-%C3%AE%C2%B5-poly-l-lysine-based-hydrogels-with-robust-self-healing-and-antibacterial-performance
#9
Rui Wang, Qing Li, Bo Chi, Xiaoqiao Wang, Zheng Xu, Zongqi Xu, Su Chen, Hong Xu
An enzyme-induced strategy is reported to construct novel self-mending hydrogels based on ε-poly-l-lysine with both excellent self-healing properties (95%) and antibacterial capacity. Most importantly, the hydrogels are able to accelerate wound healing efficiently, which shows great potential in myriad biomedical fields, such as wound repair, artificial skin, and tissue engineering.
April 13, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28400198/vitamin-b12-loaded-buccoadhesive-films-as-a-non-invasive-supplement-in-vitamin-b12-deficiency-in-vitro-evaluation-and-in-vivo-comparative-study-with-intramuscular-injection
#10
Soad A Mohamad, Hatem A Sarhan, Hamdy Abdelkader, Heba F Mansour
This study aimed to formulate and evaluate vitamin B12- loaded buccal mucoadhesive hydrogel films. Various film formulations were prepared using chitosan and polyvinyl alcohol. The prepared films were characterized for thickness, weight variation, drug content, percentage moisture uptake and moisture content, surface pH, mechanical properties, in vitro release and mucoadhesion. Vitamin B12 bioavailability from the optimized formulation was studied on rabbits by the aid of enzyme-linked immunosorbent assay (ELISA)...
April 8, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28391052/bio-orthogonal-conjugation-and-enzymatically-triggered-release-of-proteins-within-multi-layered-hydrogels
#11
Chen Guo, Heejae Kim, Elisa M Ovadia, Christine M Mourafetis, Mingrui Yang, Wilfred Chen, April M Kloxin
Hydrogels are facile architectures for the controlled presentation of proteins with far-reaching applications, from fundamental biological studies in three-dimensional culture to new regenerative medicine and therapeutic delivery strategies. Here, we demonstrate a versatile approach for spatially-defined presentation of engineered proteins within hydrogels through i) immobilization using bio-orthogonal strain-promoted alkyne-azide click chemistry and ii) dynamic proteasedriven protein release using exogenously applied enzyme...
April 5, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28376299/enzymatic-inverse-opal-hydrogel-particles-for-biocatalyst
#12
Huan Wang, Hongcheng Gu, Zhuoyue Chen, Luoran Shang, Ze Zhao, Zhongze Gu, Yuanjin Zhao
Enzymatic carriers have a demonstrated value for chemical reactions and industrial applications. Here, we present a novel kind of inverse opal hydrogel particles as the enzymatic carriers. The particles were negatively replicated from spherical colloidal crystal templates by using magnetic nanoparticles tagged acrylamide hydrogel. Thus, they were endowed with the features of monodispersity, small volume, complete penetrating structure, and controllable motion, which are all beneficial for improving the efficiency of biocatalysis...
April 6, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28371450/hydrogel-based-biosensors-for-in-vitro-dia-of-biochemicals-proteins-and-genes
#13
REVIEW
Il Young Jung, Ji Su Kim, Bo Ram Choi, Kyuri Lee, Hyukjin Lee
Hydrogel-based biosensors have drawn considerable attention due to their various advantages over conventional detection systems. Recent studies have shown that hydrogel biosensors can be excellent alternative systems to detect a wide range of biomolecules, including small biochemicals, pathogenic proteins, and disease specific genes. Due to the excellent physical properties of hydrogels such as the high water content and stimuli-responsive behavior of cross-linked network structures, this system can offer substantial improvement for the design of novel detection systems for various diagnostic applications...
March 31, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28351680/human-lung-fibroblast-derived-matrix-facilitates-vascular-morphogenesis-in-3d-environment-and-enhances-skin-wound-healing
#14
Ping Du, Muhammad Suhaeri, Sang Su Ha, Seung Ja Oh, Sang Heon Kim, Kwideok Park
Extracellular matrix (ECM) is crucial to many aspects of vascular morphogenesis and maintenance of vasculature function. Currently the recapitulation of angiogenic ECM microenvironment is still challenging, due mainly to its diverse components and complex organization. Here we investigate the angiogenic potential of human lung fibroblast-derived matrix (hFDM) in creating a three-dimensional (3D) vascular construct. hFDM was obtained via decellularization of in vitro cultured human lung fibroblasts and analyzed via immunofluorescence staining and ELISA, which detect multiple ECM macromolecules and angiogenic growth factors (GFs)...
March 25, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28351221/understanding-the-spatiotemporal-degradation-behavior-of-aggrecanase-sensitive-poly-ethylene-glycol-hydrogels-for-use-in-cartilage-tissue-engineering
#15
Stanley Chu, Shankar Lalitha Sridhar, Stacey C Skaalure, Franck J Vernerey, Stephanie J Bryant
Enzyme-sensitive hydrogels are promising cell delivery vehicles for cartilage tissue engineering. However, a better understanding of their spatiotemporal degradation behavior and its impact on tissue growth is needed. The goal of this study is to combine experimental and computational approaches to provide new insights into spatiotemporal changes in hydrogel crosslink density and extracellular matrix (ECM) growth and how these changes influence the evolving macroscopic properties as a function of time. Hydrogels were designed from aggrecanase-sensitive peptide crosslinks using a simple and robust thiol:norbornene photoclick reaction...
March 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28345345/continuously-electrotriggered-core-coalescence-of-double-emulsion-drops-for-microreactions
#16
Likai Hou, Yukun Ren, Yankai Jia, Xiaokang Deng, Weiyu Liu, Xiangsong Feng, Hongyuan Jiang
Microfluidically generated double emulsions are promising templates for microreactions, which protect the reaction from external disturbance and enable in vitro analyses with large-scale samples. Controlled combination of their inner droplets in a continuous manner is an essential requirement toward truly applications. Here, we first generate dual-cored double-emulsion drops with different inner encapsulants using a capillary microfluidic device; next, we transfer the emulsion drops into another electrode-integrated polydimethylsiloxane microfluidic device and utilize external AC electric field to continuously trigger the coalescence of inner cores inside these emulsion drops in continuous flow...
March 30, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28345030/wearable-disposable-sweat-based-glucose-monitoring-device-with-multistage-transdermal-drug-delivery-module
#17
Hyunjae Lee, Changyeong Song, Yong Seok Hong, Min Sung Kim, Hye Rim Cho, Taegyu Kang, Kwangsoo Shin, Seung Hong Choi, Taeghwan Hyeon, Dae-Hyeong Kim
Electrochemical analysis of sweat using soft bioelectronics on human skin provides a new route for noninvasive glucose monitoring without painful blood collection. However, sweat-based glucose sensing still faces many challenges, such as difficulty in sweat collection, activity variation of glucose oxidase due to lactic acid secretion and ambient temperature changes, and delamination of the enzyme when exposed to mechanical friction and skin deformation. Precise point-of-care therapy in response to the measured glucose levels is still very challenging...
March 2017: Science Advances
https://www.readbyqxmd.com/read/28330754/injectable-nanoparticles-hydrogels-composite-as-sustained-release-system-with-stromal-cell-derived-factor-1%C3%AE-for-calvarial-bone-regeneration
#18
Lei Mi, Huaiqin Liu, Yu Gao, Hui Miao, Jianping Ruan
Repair of craniofacial bony defects remains a challenge for surgeons due to the delicate and complex anatomy of the craniofacial skeleton. Stromal cell-derived factor-1α (SDF-1α) is an important chemokine which plays a critical role in the homing of mesenchymal stem cells (MSC), while, the shortcomings including short half-life and easy degradation by enzymes made it in relatively low efficacy. In this work, SDF-1α/chitosan/carboxymeymethy-chitosan nanoparticles (SDF-1α/CS/CMCS NPs) were prepared and characterized for various parameters including morphology, particle size, zeta potential, loading efficiency and the release characteristics from thermosensitive chitosan/β-glycerol phosphate disodium salt (CS/GP) hydrogels...
March 19, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28321425/adult-cells-combined-with-platelet-rich-plasma-for-tendon-healing-cell-source-options
#19
Eva Rubio-Azpeitia, Pello Sánchez, Diego Delgado, Isabel Andia
BACKGROUND: The combination of cells with platelet-rich plasma (PRP) may fulfill tendon deficits and help overcome the limited ability of tendons to heal. PURPOSE: To examine the suitability of 3 human cell types in combination with PRP and the potential impact of the tenocyte-conditioned media (CM) to enhance tendon healing. STUDY DESIGN: Controlled laboratory study. METHODS: Tenocytes, bone marrow-derived mesenchymal stem cells, and skin fibroblasts were cultured in 3-dimensional PRP hydrogels supplemented or not with CM, and cell proliferation and migration were examined...
February 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28301717/glucose-triggered-insulin-release-from-fe3-cross-linked-alginate-hydrogel-experimental-study-and-theoretical-modeling
#20
Evgeny Katz, Sabrina Scheja, Sergii Domanskyi, Maria Gamella, Kelly L Wormwood, Costel C Darie, Arshak Poghossian, Michael J Schöning, Artem Melman, Vladimir Privman
We study the mechanisms involved in the release, triggered by the application of glucose, of insulin entrapped in Fe3+-cross-linked alginate hydrogel particles further stabilized with a polyelectrolyte. Platelet-shaped alginate particles are synthesized containing enzyme glucose oxidase conjugated with silica nanoparticles, which are also entrapped in the hydrogel. Glucose diffuses in from solution, and production of hydrogen peroxide is catalyzed by the enzyme within the hydrogel. We argue that, specifically for the Fe3+-cross-linked systems, the produced hydrogen peroxide is further converted to free radicals via a Fenton-type reaction catalyzed by the iron cations...
March 16, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
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