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Enzyme hydrogel

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https://www.readbyqxmd.com/read/28919509/molecular-and-macro-scale-analysis-of-enzyme-crosslinked-silk-hydrogels-for-rational-biomaterial-design
#1
Meghan McGill, Jeannine M Coburn, Benjamin P Partlow, Xuan Mu, David L Kaplan
Silk fibroin-based hydrogels have exciting applications in tissue engineering and therapeutic molecule delivery; however, their utility is dependent on their diffusive properties. The present study describes a molecular and macro-scale investigation of enzymatically-crosslinked silk fibroin hydrogels, and demonstrates that these systems have tunable crosslink density and diffusivity. We developed a liquid chromatography tandem mass spectroscopy (LC-MS/MS) method to assess the quantity and order of covalent tyrosine crosslinks in the hydrogels...
September 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28901673/catanionic-coacervate-micro-droplets-as-a-surfactant-based-membrane-free-protocell-model
#2
Jean-Paul Douliez, Nicolas Martin, Stephen Mann, Cédric Gaillard, Thomas Beneyton, Jean-Christophe Baret, Laure Beven
We report on the formation of surfactant complex catanionic coacervate micro-droplets in mixtures of decanoic acid and cetyl pyridinium chloride or cetyl trimethyl ammonium bromide. We show that coacervation occurs over a broad range of composition, pH and ionic strength. The catanionic coacervates consist of elongated micelles, sequester a wide range of solutes including water-soluble organic dyes, polysaccharides, proteins, enzymes and DNA, and can be structurally stabilized by sodium alginate or gelatin-based hydrogelation...
September 13, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28880715/defective-mineralization-in-x-linked-hypophosphatemia-dental-pulp-cell-cultures
#3
B R Coyac, B Hoac, P Chafey, G Falgayrac, L Slimani, P S Rowe, G Penel, A Linglart, M D McKee, C Chaussain, C Bardet
X-linked hypophosphatemia (XLH) is a skeletal disease caused by inactivating mutations in the PHEX gene. Mutated or absent PHEX protein/enzyme leads to a decreased serum phosphate level, which cause mineralization defects in the skeleton and teeth (osteomalacia/odontomalacia). It is not yet altogether clear whether these manifestations are caused solely by insufficient circulating phosphate availability for mineralization or also by a direct, local intrinsic effect caused by impaired PHEX activity. Here, we evaluated the local role of PHEX in a 3-dimensional model of extracellular matrix (ECM) mineralization...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28872843/diffusion-controllable-biomineralization-conducted-in-situ-in-hydrogels-based-on-reversibly-cross-linked-hyperbranched-polyglycidol
#4
Mateusz Gosecki, Slawomir Kazmierski, Monika Gosecka
We present biocompatible hydrogel systems suitable for biomineralization processes based on hyperbranched polyglycidol cross-linked with acrylamide copolymer bearing carbonyl-coordinated boronic acid. At neutral pH, diol functional groups of HbPGL react with boronic acid of polyacrylamide to generate 3D network in water by the formation of boronic ester cross-links. The dynamic associative/dissociative characteristics of the cross-links makes the network reversible. The presented hydrogels display self-healing properties and are injectable, facilitating gap filing of bone tissue...
September 13, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28869865/visual-in%C3%A2-vivo-degradation-of-injectable-hydrogel-by-real-time-and-non-invasive-tracking-using-carbon-nanodots-as-fluorescent-indicator
#5
Lei Wang, Baoqiang Li, Feng Xu, Ying Li, Zheheng Xu, Daqing Wei, Yujie Feng, Yaming Wang, Dechang Jia, Yu Zhou
Visual in vivo degradation of hydrogel by fluorescence-related tracking and monitoring is crucial for quantitatively depicting the degradation profile of hydrogel in a real-time and non-invasive manner. However, the commonly used fluorescent imaging usually encounters limitations, such as intrinsic photobleaching of organic fluorophores and uncertain perturbation of degradation induced by the change in molecular structure of hydrogel. To address these problems, we employed photoluminescent carbon nanodots (CNDs) with low photobleaching, red emission and good biocompatibility as fluorescent indicator for real-time and non-invasive visual in vitro/in vivo degradation of injectable hydrogels that are mixed with CNDs...
August 26, 2017: Biomaterials
https://www.readbyqxmd.com/read/28858720/fret-enabled-monitoring-of-the-thermosensitive-nanoscale-assembly-of-polymeric-micelles-into-macroscale-hydrogel-and-sequential-cognate-micelles-release
#6
Pingsheng Huang, Huijuan Song, Yumin Zhang, Jinjian Liu, Zhen Cheng, Xing-Jie Liang, Weiwei Wang, Deling Kong, Jianfeng Liu
Thermosensitive "micellar hydrogel" is prepared based on poly(ε-caprolactone-co- 1,4,8-trioxa[4.6]spiro-9-undecanone)-b-poly(ethylene glycol)- b-poly(ε-caprolactone- co-1,4,8-trioxa[4.6]spiro-9-undecanone) (PECT) triblock copolymer. Fluorescence resonance energy transfer (FRET) is adopted to explore its assembly (formation) and disassembly (degradation) mechanism within the range of 10 nm. Results prove that the thermosensitive non-covalent aggregation of micelles facilitates the hydrogel formation and the sustained shedding of cognate micelles induces the hydrogel degradation, during which polymers are steadily incorporated in micelles without any micelle disassembly or reassembly...
July 10, 2017: Biomaterials
https://www.readbyqxmd.com/read/28851869/multifunctional-quantum-dot-dna-hydrogels
#7
Libing Zhang, Sae Rin Jean, Sharif Ahmed, Peter M Aldridge, Xiyan Li, Fengjia Fan, Edward H Sargent, Shana O Kelley
Biotemplated nanomaterials offer versatile functionality for multimodal imaging, biosensing, and drug delivery. There remains an unmet need for traceable and biocompatible nanomaterials that can be synthesized in a precisely controllable manner. Here, we report self-assembled quantum dot DNA hydrogels that exhibit both size and spectral tunability. We successfully incorporate DNA-templated quantum dots with high quantum yield, long-term photostability, and low cytotoxicity into a hydrogel network in a single step...
August 29, 2017: Nature Communications
https://www.readbyqxmd.com/read/28827094/injectable-hydrogels-for-sustained-release-of-therapeutic-agents
#8
Thavasyappan Thambi, Yi Li, Doo Sung Lee
Hydrogels are natural or synthetic polymer networks that exhibit high water absorbent capacities and have been used as scaffolds for tissue engineering or as delivery carriers for therapeutic agents and cells. Owing to their tunable physicochemical properties, hydrogels can provide spatial and temporal control over the release of loaded therapeutic agents, including chemotherapeutic drugs, proteins or cells. In particular, in situ-forming injectable hydrogels, the state-of-the-art clear free flowing polymer solutions that transform to viscoelastic gels upon exposure to stimuli including pH, temperature, light, enzymes and magnetic field, have been widely studied as delivery carriers for therapeutic agents...
August 4, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28815227/microparticle-parking-and-isolation-for-highly-sensitive-microrna-detection
#9
Jae Jung Kim, Lynna Chen, Patrick S Doyle
Isolating small objects, such as particles, cells, and molecules, in individual aqueous droplets is useful for chemical and biological assays. We have developed a simple microfluidic platform to immobilize (park) microparticles at defined locations, and isolate particles in monodisperse droplets surrounded by immiscible oil. While conventional methods can only achieve stochastic encapsulation of objects within larger droplets, our in situ method ensures that a single particle is entrapped in a similar-sized droplet, with ∼95% yield for parking and isolation...
September 12, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28806066/thiol-mediated-synthesis-of-hyaluronic-acid-epigallocatechin-3-o-gallate-conjugates-for-the-formation-of-injectable-hydrogels-with-free-radical-scavenging-property-and-degradation-resistance
#10
Chixuan Liu, Ki Hyun Bae, Atsushi Yamashita, Joo Eun Chung, Motoichi Kurisawa
Hyaluronic acid (HA)-based biomaterials have demonstrated only limited in vivo stability as a result of rapid degradation by hyaluronidase and reactive oxidative species. The green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has received considerable attention because of its powerful antioxidant and enzyme-inhibitory activities. We describe here the synthesis of HA-EGCG conjugate using a thiol-mediated reaction and its use for the preparation of a long-lasting injectable hydrogel. HA-EGCG conjugates with tunable degrees of substitution were synthesized by the nucleophilic addition reaction between EGCG quinone and thiolated HA under mild conditions...
September 5, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28792044/enzyme-assisted-peptide-folding-assembly-and-anti-cancer-properties
#11
Chunhui Liang, Debin Zheng, Fang Shi, Tengyan Xu, Cuihong Yang, Jianfeng Liu, Ling Wang, Zhimou Yang
The α-helix is the most prevalent conformation in proteins. However, formation of the α-helical conformation remains a challenge for short peptides with less than 5 amino acids. We demonstrated in this study that enzyme-instructed self-assembly (EISA) provides a unique pathway to assist the self-assembly of peptides into the α-helical conformation, while a heating-cooling process leads to a conformation more similar to a β-sheet. The same peptide with different conformations self-assembled into different nanostructures...
August 24, 2017: Nanoscale
https://www.readbyqxmd.com/read/28765593/folding-driven-self-assembly-of-a-stimuli-responsive-peptide-hyaluronan-hybrid-hydrogel
#12
Robert Selegård, Christopher Aronsson, Caroline Brommesson, Staffan Dånmark, Daniel Aili
Protein-metal ion interactions are ubiquitous in nature and can be utilized for controlling the self-assembly of complex supramolecular architectures and materials. Here, a tunable supramolecular hydrogel is described, obtained by self-assembly of a Zn(2+)-responsive peptide-hyaluronic acid hybrid synthesized using strain promoted click chemistry. Addition of Zn(2+) triggers folding of the peptides into a helix-loop-helix motif and dimerization into four-helix bundles, resulting in hydrogelation. Removal of the Zn(2+) by chelators results in rapid hydrogel disassembly...
August 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28752299/silica-microparticles-for-sustained-zero-order-release-of-an-anti-cd40l-antibody
#13
Puneet Tyagi, Mika Koskinen, Jari Mikkola, Lasse Leino, Alexander Schwarz
Silica microparticle hydrogel depot (HG) formulation was prepared using spray drying of silica-based sol-gels for the sustained delivery of MR1 antibody which binds to CD40 ligand (CD40L). The formulation was tested in vitro for antibody release, surface morphology, particle size, rheology, and injectability. In vivo pharmacokinetic evaluation was performed for the microparticle formulation and free MR1 antibody in BALB/c female mice. Serum samples up to day 62 were assessed using an enzyme-linked immunosorbent assay...
July 27, 2017: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/28742363/improved-performance-of-glucose-bioanodes-using-composites-of-7-6-single-walled-carbon-nanotubes-and-a-ferrocene-lpei-redox-polymer
#14
Jie Chen, Rujuta Munje, Nicholas P Godman, Shalini Prasad, Daniel T Glatzhofer, David W Schmidtke
The effect of incorporating different types of carbon nanotubes into composite films of a redox polymer (FcMe2-C3-LPEI) and glucose oxidase (GOX) was investigated. The composite films were constructed by first forming a high-surface area network film of either single-walled carbon nanotubes (SWNTs) or multiwalled carbon nanotubes (MWNTs) on a glassy carbon electrode (GCE) by solution casting of a suspension of Triton-X-100 dispersed SWNTs. Next a glucose responsive redox hydrogel was formed on top of the nanotube-modified electrode by cross-linking FcMe2-C3-LPEI with glucose oxidase via ethylene glycol diglycidyl ether (EGDGE)...
July 25, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28738228/streptavidin-hydrogel-prepared-by-sortase-a-assisted-click-chemistry-for-enzyme-immobilization-on-an-electrode
#15
Takuya Matsumoto, Yuki Isogawa, Tsutomu Tanaka, Akihiko Kondo
A tetrameric streptavidin (SA)-appended LPETG tag was site-specifically linked to azido-containing tri-glycine via sortase A catalysis and the resulting azido-modified SA (SA-N3) was retained in the biotin-binding pocket. SA-N3 was polymerized with dibenzylcyclooctyne-modified branched poly(ethyleneglycol) (DBCO-PEG) using azido-modified branched PEG (N3-PEG) as a spacer via copper-free click chemistry. The resulting SA-based hydrogel exhibited gel-like mechanical properties and could immobilize biotin-modified molecules through biotin-SA affinity...
July 19, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28736041/construction-of-multilayer-alginate-hydrogel-beads-for-oral-delivery-of-probiotics-cells
#16
Yang Li, Chao Feng, Jing Li, Yuzhi Mu, Ya Liu, Ming Kong, Xiaojie Cheng, Xiguang Chen
Multilayer alginate hydrogel beads (MAHBs), which were prepared by emulsion method, had been fabricated via the ionic crosslinking between calcium ion (Ca(2+)) and carboxylic group of alginate and utilized as encapsulating material for oral delivery of a model probiotic bacteria Bifidobacterium breve. Optical and fluorescence microscopy obviously showed the microorganism cells were encapsulated in the core beads. The viability of B. breve cells encapsulated in MAHBs was significantly enhanced compared with ordinary free cultured ones, the top CFU per mL were (3...
July 20, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28729217/preventing-postoperative-tissue-adhesion-using-injectable-carboxymethyl-cellulose-pullulan-hydrogels
#17
Sumi Bang, Young-Gwang Ko, Won Il Kim, Donghwan Cho, Won Ho Park, Oh Hyeong Kwon
An injectable adhesive hydrogel composed of carboxymethyl cellulose (CMC) and pullulan is developed and evaluated as a postoperative anti-adhesion barrier. CMC was modified with tyramine to introduce crosslinking via an EDC-NHS reaction. The in situ hydrogel was developed by an enzyme-mediated reaction of tyramine-immobilized CMC with horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Pullulan was added to the hydrogel solution to improve adhesiveness to the wound area and accelerate biodegradation...
July 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28722424/metabolic-study-of-cancer-cells-using-a-ph-sensitive-hydrogel-nanofiber-light-addressable-potentiometric-sensor
#18
Parmiss Mojir Shaibani, Hashem Etayash, Selvaraj Naicker, Kamaljit Kaur, Thomas Thundat
We report a simple, fast, and cost-effective approach that measures cancer cell metabolism and their response to anticancer drugs in real time. Using a Light Addressable Potentiometric Sensor integrated with pH sensitive hydrogel nanofibers (NF-LAPS), we detect localized changes in pH of the media as cancer cells consume glucose and release lactate. NF-LAPS shows a sensitivity response of 74 mV/pH for cancer cells. Cancer cells (MDA MB231) showed a response of ∼0.4 unit change in pH compared to virtually no change observed for normal cells (MCF10A)...
January 27, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28714570/human-osteoarthritic-chondrons-outnumber-patient-and-joint-matched-chondrocytes-in-hydrogel-culture-future-application-in-autologous-cell-based-oa-cartilage-repair
#19
Miriam Rothdiener, Tatiana Uynuk-Ool, Norbert Südkamp, Matthias Aurich, Alan J Grodzinsky, Bodo Kurz, Bernd Rolauffs
Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA-chondrons may outperform OA-chondrocytes. We compared patient- and joint surface-matched human OA-chondrons vs. OA-chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, collagen II synthesis (CPII) and degradation (C2C), and sGAG...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28707693/single-step-fabrication-of-multi-compartmentalized-biphasic-proteinosomes
#20
Lei Wang, Ping Wen, Xiaoman Liu, Yuting Zhou, Mei Li, Yudong Huang, Lin Geng, Stephen Mann, Xin Huang
Multi-compartmentalized biphasic proteinosomes were self-assembled using a single-step double Pickering emulsion procedure, and exploited for enzyme-mediated interfacial catalysis, polysaccharide shell templating, and hydrogel functionalization.
July 27, 2017: Chemical Communications: Chem Comm
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