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https://www.readbyqxmd.com/read/28225975/descriptive-analysis-of-mycological-examination-of-patients-with-onychomycosis-treated-in-private-practice
#1
John Verrinder Veasey, Flávio Nappi, Clarisse Zaitz, Laura Hitomi Muramatu
This is a retrospective study of 160 patients treated in private practice in São Paulo from March 2003 to March 2015. We analyzed 171 results of direct mycological examinations and fungal cultures from nail scrapings. The agreement between direct mycological examination results and fungal culture was satisfactory, consistent with the literature (kappa 0.603). The main agent identified was Trichophyton rubrum (51%). We observed an isolation rate of non-dermatophyte filamentous fungi superior to the literature (34%)...
January 2017: Anais Brasileiros de Dermatologia
https://www.readbyqxmd.com/read/28223741/changing-in-the-epidemiology-of-tinea-capitis-among-school-children-in-egypt
#2
Rasha H Bassyouni, Naglaa A El-Sherbiny, Talal A Abd El Raheem, Basma H Mohammed
BACKGROUND: Tinea capitis remains a prevalent health problem among school-aged children. OBJECTIVE: To estimate the prevalence of tinea capitis among primary school students, in Fayoum, Egypt with identification of etiological agents in both public and private primary schools. METHODS: A cross-sectional study was conducted in twelve primary schools. The students were selected from different grades with a total number of 12,128 students. Hair and scalp were clinically examined for any lesions that may suspect tinea capitis and mycological samples were collected for direct microscopy and culture...
February 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/28218268/rapid-and-sensitive-diagnoses-of-dry-root-rot-pathogen-of-chickpea-rhizoctonia-bataticola-taub-butler-using-loop-mediated-isothermal-amplification-assay
#3
Raju Ghosh, Avijit Tarafdar, Mamta Sharma
Dry root rot (DRR) caused by the fungus Rhizoctonia bataticola (Taub.) Butler, is an emerging disease in chickpea. The disease is often mistaken with other root rots like Fusarium wilt, collar rot and black root rot in chickpea. Therefore, its timely and specific detection is important. Current detection protocols are either based on mycological methods or on protocols involving DNA amplification by polymerase chain reaction (PCR). Here we report the rapid and specific detection of R. bataticola using loop-mediated isothermal amplification (LAMP) assay targeting fungal specific 5...
February 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28214274/intra-abdominal-fungal-pseudomycetoma-in-two-cats
#4
Matheus V Bianchi, Cláudio J M Laisse, Thainã P Vargas, Flademir Wouters, Fabiana M Boabaid, Saulo P Pavarini, Laerte Ferreiro, David Driemeier
BACKGROUND: Pseudomycetomas are deep cutaneous to subcutaneous lesions caused by Microsporum canis mainly described in Persian cats, with few reports of intra-abdominal location. This report describes the clinical signs and lesions of intra-abdominal pseudomycetomas caused by M. canis in two Persian cats. CASE REPORT: Two Persian cats with a history of previous laparotomy (ovariohysterectomy and nephrostomy) and fecal impaction were examined. Cat #1 was euthanized and subjected to necropsy, histopathology and mycological evaluation...
February 14, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28214142/-a-case-of-rhinofacial-entomophthoromycosis-in-soudano-sahelian-tropical-climate-in-burkina-faso
#5
S Bamba, V Konsegré, A Zida, I Sangaré, M Cissé, R Beogo, B Diallo, J B Andonaba, R T Guiguemdé
We describe a rhinofacial entomophthoramycosis case in a sexagenarian (65 years old) housewife. She was immunocompetent and resident of Burkina Faso. She consulted both the service of dermatology and the service of stomatology of the Teaching Hospital of Bobo-Dioulasso in February 2016 for a diffuse facial tumefaction evolving over six months. This tumefaction was associated with headaches and a left nasal obstruction. Histological examination of the lesion showed an important and polymorphic inflammatory reaction...
February 14, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28210388/topical-treatment-for-onychomycosis-is-it-more-effective-than-the-clinical-data-suggests
#6
REVIEW
Boni E Elewski, Tracey C Vlahovic, Andrew Korotzer
Background: The current definition of complete cure is considered too stringent to reflect the true benefit of onychomycosis treatment seen in general practice and may limit the use of newer topical agents in mild-to-moderate disease. In addition, outcomes reported in clinical trials do not consistently report secondary endpoints, making data comparison difficult. Methods: The authors review the clinical data reported on two new topical antifungals, efinaconazole and tavaborole, in light of the latest thinking of more practical approaches to assess improvement and treatment success...
November 2016: Journal of Clinical and Aesthetic Dermatology
https://www.readbyqxmd.com/read/28188648/pilot-study-to-evaluate-a-plasma-device-for-the-treatment-of-onychomycosis
#7
S R Lipner, G Friedman, R K Scher
Onychomycosis is a fungal infection of the nail unit, and is the most common of the nail disorders. Current therapies for onychomycosis have less than ideal efficacy and have the potential for adverse effects. As previous studies have shown that nonthermal plasma inhibits the in vitro growth of Trichophyton rubrum, we conducted a pilot study on 19 participants with toenail onychomycosis. The primary endpoint was safety of the device, and secondary outcome measures were clinical efficacy and mycological cure...
February 11, 2017: Clinical and Experimental Dermatology
https://www.readbyqxmd.com/read/28188051/incidence-and-biodiversity-of-yeasts-dermatophytes-and-non-dermatophytes-in-superficial-skin-infections-in-assiut-egypt
#8
A H Moubasher, M A Abdel-Sater, Z Soliman
OBJECTIVE: The aim was to identify the incidence of the causal agents from dermatophytes, non-dermatophytes and yeasts in Assiut Governorate employing, beside the morphological and physiological techniques, the genotypic ones. PATIENTS: Samples from infected nails, skin and hair were taken from 125 patients. MATERIALS AND METHODS: Patients who presented with onychomycosis, tinea capitis, tinea corporis, tinea cruris and tinea pedis during the period from February 2012 to October 2015 were clinically examined and diagnosed by dermatologists and were guided to Assiut University Mycological Centre for direct microscopic examination, culturing and identification...
February 7, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28179406/online-databases-for-the-taxonomy-and-identification-of-pathogenic-fungi-and-proposal-for-a-cloud-based-dynamic-data-network-platform
#9
Peralam Yegneswaran Prakash, Laszlo Irinyi, Catriona Halliday, Sharon Chen, Vincent Robert, Wieland Meyer
The increase in public online databases dedicated to fungal identification is noteworthy. This can be attributed to improved access to molecular approaches to characterize fungi, as well as to delineate species within specific fungal groups in the last two decades, leading to an ever-increasing complexity of taxonomic assortments and nomenclatural re-assignments. Thus well-curated fungal databases with substantial accurate sequence data play a pivotal role for further research and diagnostics in the field of mycology...
February 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28173801/cryptococcus-gattii-molecular-type-vgii-infection-associated-with-lung-disease-in-a-goat
#10
Evelin Catarine da Silva, Juliana Mariotti Guerra, Luciana Neves Torres, Alessandra Maria Dias Lacerda, Raquel Gonçalves Gomes, Danilo Marin Rodrigues, Rodrigo Albergaria Réssio, Priscilla Anne Melville, Camila Cecilia Martin, Fernando José Benesi, Lílian Rose Marques de Sá, Bruno Cogliati
BACKGROUND: Cryptococcus gattii-induced cryptococcosis is an emerging infectious disease of humans and animals with worldwide distribution and public health importance due to its significant morbidity and mortality rate. The present study aimed to report a case of pulmonary infection by C. gattii molecular type VGII in State of São Paulo, Brazil. CASE PRESENTATION: A 5-year-old goat showing intermittent dry cough, ruminal tympany, anorexia, fever, tachycardia and tachypnea was presented for necropsy at the Veterinary Hospital of the School of Veterinary Medicine and Animal Sciences, São Paulo University, São Paulo, Brazil...
February 7, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28155810/fungal-enzymes-and-yeasts-for-conversion-of-plant-biomass-to-bioenergy-and-high-value-products
#11
Lene Lange
Fungi and fungal enzymes play important roles in the new bioeconomy. Enzymes from filamentous fungi can unlock the potential of recalcitrant lignocellulose structures of plant cell walls as a new resource, and fungi such as yeast can produce bioethanol from the sugars released after enzyme treatment. Such processes reflect inherent characteristics of the fungal way of life, namely, that fungi as heterotrophic organisms must break down complex carbon structures of organic materials to satisfy their need for carbon and nitrogen for growth and reproduction...
January 2017: Microbiology Spectrum
https://www.readbyqxmd.com/read/28141498/first-genotype-identification-of-trichosporon-asahii-in-sfax-tunisia
#12
Hayet Sellami, Houaida Trabelsi, Sourour Neji, Imen Amouri, Fatma Cheikhrouhou, Fattouma Makni, Ali Ayadi
PURPOSE: The objectives of our study were species identification and genotyping of Trichosporon isolates collected at the Parasitology and Mycology Laboratory in Sfax, Tunisia. METHODOLOGY: Molecular identification was carried out by analyzing the IGS1 regions of the rDNA of 30 Trichosporon isolates. RESULTS: Trichosporon asahii was the most frequent species detected. Furthermore, four genotypes were identified in Tunisia: 1 (46.4%), 4 (35...
January 27, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28130196/cyclodextrin-mediated-self-associating-chitosan-micro-platelets-act-as-a-drug-booster-against-candida-glabrata-mucosal-infection-in-immunocompetent-mice
#13
Tiphany Grisin, Christian Bories, Philippe M Loiseau, Kawthar Bouchemal
This study reports design and evaluation of chitosan-based microparticle activity against Candida glabrata in vitro and in vivo in immunocompetent mice model artificially maintained in oestrus state. Because their flattened shape, chitosan microparticles are called here micro-platelets. They were obtained by self-association of oleoyl chitosan and α-cyclodextrin in water. A mixture of amphotericin B-deoxycholate (Fungizone(®), AmB-DOC) and chitosan micro-platelets gelified with pluronic(®) F127 (20wt%) completely cured C...
January 24, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28124963/-evaluation-of-pna-fish-method-for-direct-identification-of-candida-species-in-blood-culture-samples-and-its-potential-impact-on-guidance-of-antifungal-therapy
#14
Özlem Doğan, Ahmet Çağkan İnkaya, Dolunay Gülmez, Ömrüm Uzun, Murat Akova, Sevtap Arıkan Akdağlı
Early antifungal therapy has a major influence on survival in candidemia. Rapid identification of the species has importance for the treatment, prediction of the species-specific primary resistance and variable antifungal susceptibility. Recently, molecular-based methods attempt to reduce the time between the positive signal of a blood culture and identification of the fungus. PNA-FISH (Peptide nucleic acid fluorescence in situ hybridization) assay distinguishes a number of frequently isolated Candida species in groups following the growth in blood culture...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28120351/comparative-efficacy-of-topical-application-of-tacrolimus-and-clotrimazole-in-the-treatment-of-pityriasis-versicolor-a-single-blind-randomised-clinical-trial
#15
Mozhdeh Sepaskhah, Maryam Sadat Sadat, Keyvan Pakshir, Zahra Bagheri
BACKGROUND: Pityriasis versicolor (PV) is a common superficial fungal disease. Possibility of emergence of resistant strains to azoles, and difficulty in differentiation of hypopigmented PV and early vitiligo, encouraged us to evaluate the efficacy of topical tacrolimus (a calcineurin inhibitor agent with proven in vitro anti-Malassezia effect) for PV treatment generally and its effect on PV-induced hypopigmentation specifically. OBJECTIVES: To evaluate the efficacy of topical tacrolimus on pityriasis versicolor...
January 25, 2017: Mycoses
https://www.readbyqxmd.com/read/28108869/molecular-identification-of-malassezia-species-in-patients-with-malassezia-folliculitis-in-sfax-tunisia
#16
F Cheikhrouhou, R Guidara, A Masmoudi, H Trabelsi, S Neji, H Sellami, F Makni, A Ayadi
AIM: Malassezia folliculitis is caused by the invasion of hair follicles by large numbers of Malassezia cells. Several Malassezia researches still use cultures, morphology and biochemical techniques. The aim of this study was to identify Malassezia species isolated from patients diagnosed with folliculitis, at the Parasitology and Mycology Laboratory of Sfax University Hospital, and to explore the genetic diversity of Malassezia by using PCR-RFLP and PCR-sequencing targeting the rDNA region of the Malassezia genome...
January 20, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28101694/relationship-between-phenotypic-and-genotypic-characteristics-of-trichophyton-mentagrophytes-strains-isolated-from-patients-with-dermatophytosis
#17
I Dhib, I Khammari, A Yaacoub, F Hadj Slama, M Ben Saïd, R Zemni, A Fathallah
According to epidemiological, clinical and mycological criteria, it has long been admitted that the Trichophyton mentagrophytes species includes two varieties: a zoophilic variety (var. mentagrophytes) and an anthropophilic variety (var. interdigitale) that involve the upper and the lower part of the body, respectively. The further application of molecular techniques to the characterization of dermatophyte strains showed that this classification is unreliable. The aim of our study was to assess the usefulness of PCR-RFLP (restriction fragment length polymorphism) and sequencing in the characterization of T...
January 18, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28080988/medical-mycology-and-fungal-immunology-new-research-perspectives-addressing-a-major-world-health-challenge
#18
REVIEW
Neil A R Gow, Mihai G Netea
Fungi cause more than a billion skin infections, more than 100 million mucosal infections, 10 million serious allergies and more than a million deaths each year. Global mortality owing to fungal infections is greater than for malaria and breast cancer and is equivalent to that owing to tuberculosis (TB) and HIV. These statistics evidence fungal infections as a major threat to human health and a major burden to healthcare budgets worldwide. Those patients who are at greatest risk of life-threatening fungal infections include those who have weakened immunity or have suffered trauma or other predisposing infections such as HIV...
December 5, 2016: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/28057425/oral-phaeohyphomycosis-in-a-patient-with-squamocellular-carcinoma-of-the-lip-second-case-report
#19
Suanni Lemos de Andrade, André Ferraz Goiana Leal, Armando Marsden Lacerda Filho, Danielle Patrícia Cerqueira Macêdo, Maria do Carmo Carvalho de Abreu E Lima, Rejane Pereira Neves
This communication reports the second known case of oral phaeohyphomycosis in a patient with squamocellular carcinoma of the lip. The patient, an 82-year-old black woman, a former smoker (for more than 30 years), suffering from an ulcerous vegetative lesion in the middle third of the lower lip for approximately 12 months. The result of the histopathological analysis indicated carcinoma, with well-differentiated keratinized squamous cells and the presence of septate mycelial filaments. In the direct mycological examination, thick and dematiaceous septate mycelial filaments were observed...
December 22, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28050341/cutaneous-pythiosis-in-calves-an-epidemiologic-pathologic-serologic-and-molecular-characterization
#20
Guilherme Konradt, Daniele Mariath Bassuino, Matheus Viezzer Bianchi, Lismara Castro, Rafaela Albuquerque Caprioli, Saulo Petinatti Pavarini, Janio M Santurio, Maria Isabel Azevedo, Francielli P Jesus, David Driemeier
This study reports the epidemiological, pathological and mycological findings of cutaneous pythiosis in cattle in southern Brazil. 23 calves, that were kept next to a river with extensive marshy regions, presented ulcerated cutaneous lesions in thoracic and pelvic limbs, sometimes extending to the ventral thoracic region. Histopathological examination revealed multifocal pyogranulomas in the superficial and deep dermis. The Grocott-Methenamine silver, immunohistochemistry anti-Pythium insidiosum, ELISA serology and molecular characterization demonstrated the agent P...
December 2016: Medical Mycology Case Reports
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