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https://www.readbyqxmd.com/read/28748285/toward-the-standardization-of-mycological-examination-of-sputum-samples-in-cystic-fibrosis-results-from-a-french-multicenter-prospective-study
#1
Noémie Coron, Marc Pihet, Emilie Fréalle, Yolande Lemeille, Claudine Pinel, Hervé Pelloux, Gilles Gargala, Loic Favennec, Isabelle Accoceberry, Isabelle Durand-Joly, Frédéric Dalle, Frédéric Huet, Annlyse Fanton, Amale Boldron, Guy-André Loeuille, Philippe Domblides, Bérengère Coltey, Isabelle Pin, Catherine Llerena, Françoise Troussier, Christine Person, Christophe Marguet, Nathalie Wizla, Caroline Thumerelle, Dominique Turck, Stéphanie Bui, Michael Fayon, Alain Duhamel, Anne Prévotat, Benoit Wallaert, Sylvie Leroy, Jean-Philippe Bouchara, Laurence Delhaes
Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: "MucoFong" program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media...
July 26, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28741338/fungi-of-the-genus-rhodotorula-isolated-from-the-oral-cavity-of-oncologic-patients-with-colorectal-cancer
#2
Piotr Troska, Ewa Sucharzewska, Maria Dynowska, Elżbieta Ejdys
This paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus Rhodotorula isolated from the oral cavity of patients with colorectal cancer. Swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and API Zym test by BioMereux. The fungi of the genus Rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. They included mono-species isolates of: Rh...
2017: Annals of Parasitology
https://www.readbyqxmd.com/read/28733568/mycology-of-onychomycosis-a-5-year-retrospective-review-2011-2015-in-hospital-kuala-lumpur
#3
R Ramalingam, S Kunalan, M M Tang
Onychomycosis is a common nail disease with numerous etiological pathogens. In order to determine and trend the local mycological pattern of culture-positive diseased nail samples sent from the Department of Dermatology, Hospital Kuala Lumpur, a five-year retrospective audit was carried out, which revealed that non-dermatophyte molds were the predominant fungi isolated, followed by yeasts and dermatophytes. This is similar to two previous studies in Malaysia, but varies greatly from other studies around the world which showed a dermatophyte-predominant prevalence...
June 2017: Medical Journal of Malaysia
https://www.readbyqxmd.com/read/28726722/comparison-of-the-results-of-studies-of-air-pollution-fungi-using-the-sas-super-100-mas-100-and-air-ideal
#4
Cecylia Łukaszuk, Elżbieta Krajewska-Kułak, Andrzej Guzowski, Wojciech Kułak, Bogumiła Kraszyńska
Although several air sampling devices for identifying and enumerating airborne microorganisms are commercially available, each poses some limitations. The aim of this study was to evaluate air pollution fungi using three such samplers: SAS Super 100, Microbiological Air Sampler (MAS) 100, and Air IDEAL. Mycological air was taken from the cellars of a 17th-century church in Siemiatycze, Poland, and the nearby outdoor environment. With samplers placed 1.5 m above the floor, microbial flora in air samples collected inside and outside the cellar were detected...
July 20, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28723316/when-green-and-red-mycology-meet-impressions-from-an-interdisciplinary-forum-on-virulence-mechanisms-of-phyto-and-human-pathogenic-fungi
#5
Yidong Yu, Bernhard Hube, Jörg Kämper, Vera Meyer, Sven Krappmann
Fungal infections pose a constant threat to plants and humans, but detailed knowledge about pathogenesis, immunity, or virulence is rather scarce. Due to the fact that a certain overlap in the armoury of infection exists between plant- and human-pathogenic fungi, an interdisciplinary forum was held in October 2016 at the Institute for Clinical Microbiology, Immunology and Hygiene in Erlangen under the organisational umbrella from two special interest groups of German microbial societies. Scientific exchange and intense discussion of this timely topic was fostered by bringing together renowned experts in their respective fields to present their thoughts and recent findings in the course of a plenary lecture and six themed sessions, accompanied by oral and poster contributions of young researchers...
July 19, 2017: Virulence
https://www.readbyqxmd.com/read/28722596/therapeutic-response-in-adult-patients-with-nonsevere-chronic-paracoccidioidomycosis-treated-with-sulfamethoxazole-trimethoprim-a-retrospective-study
#6
Andreia F Nery, Natasha P Crepaldi, Soraya B R S Rossi, Tomoko Tadano, Fabio A Leal-Santos, Rosane Christine Hahn, Valfredo M Menezes, Cor Jesus F Fontes
According to the Brazilian Consensus on Paracoccidioidomycosis (PCM), itraconazole is the drug of choice for treatment. However, the combination of sulfamethoxazole and trimethoprim (SMX-TMP) is most commonly used in clinical practice because of its higher availability in the public health services. The aims of this study were to evaluate the therapeutic response of patients with nonsevere chronic PCM to SMX-TMP and highlight the factors related to treatment failure. An adequate therapeutic response was defined as completely improved disease signs and symptoms after medication use for a minimum of 6 months, followed by normalized hematological and biochemical changes, radiological improvements, and negative mycological examination findings...
May 30, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28722380/-algorithm-to-facilitate-the-management-of-onychomycosis
#7
Guillaume-Alexandre Roux, Florence Baudraz-Rosselet
An algorithm is proposed to facilitate the management of onychomycosis and ensure a good therapeutical approach. Its aim is to simplify the diagnosis process by integrating a pertinent anamnesis and physical examination with the classic tool of mycology (direct examination, culture, eventually examination by PCR), in order to give the adequate targeted treatment.
March 29, 2017: Revue Médicale Suisse
https://www.readbyqxmd.com/read/28707751/oral-antifungal-medication-for-toenail-onychomycosis
#8
REVIEW
Sanne Kreijkamp-Kaspers, Kate Hawke, Linda Guo, George Kerin, Sally Em Bell-Syer, Parker Magin, Sophie V Bell-Syer, Mieke L van Driel
BACKGROUND: Fungal infection of the toenails, also called onychomycosis, is a common problem that causes damage to the nail's structure and physical appearance. For those severely affected, it can interfere with normal daily activities. Treatment is taken orally or applied topically; however, traditionally topical treatments have low success rates due to the nail's physical properties. Oral treatments also appear to have shorter treatment times and better cure rates. Our review will assist those needing to make an evidence-based choice for treatment...
July 14, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28706389/current-mycological-profile-of-onychomycosis-in-kashmir-valley-a-hospital-based-study
#9
Nazir Asifa, Kanth Farhath
INTRODUCTION: Onychomycosis is a common nail ailment associated with significant physical and psychological morbidity. It is caused by dermatophytes, nondermatophytic molds, and yeasts. Increased prevalence in the recent years is attributed to enhanced longevity, comorbid conditions such as diabetes, avid sports participation, and emergence of HIV. AIMS AND OBJECTIVES: This study was undertaken to identify the mycological profile and etiological agents associated with this infection...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28705753/invasive-fungal-infections-secondary-to-traumatic-injury
#10
REVIEW
Ryan Kronen, Stephen Y Liang, Grant Bochicchio, Kelly Bochicchio, William G Powderly, Andrej Spec
Invasive fungal infection (IFI) is a rare but serious complication of traumatic injury. The purpose of this article is to review the epidemiology, natural history, mycology, risk factors, diagnosis, treatment, and outcomes associated with post-traumatic IFI in military and civilian populations. The epidemiology of post-traumatic IFI is poorly characterized, but incidence appears to be rising. Patients often suffer from severe injuries and require extensive medical interventions. Fungi belonging to the order Mucorales are responsible for most post-traumatic IFI in both civilian and military populations...
July 10, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28705685/-fungal-sinusitis
#11
REVIEW
E Lafont, C Aguilar, P Vironneau, R Kania, A Alanio, S Poirée, O Lortholary, F Lanternier
Although sinusitis affects about 20 % of the population, fungal sinusitis is rare. Aspergillus sp. are most frequently implicated. Fungal sinusitis represents a wide spectrum of disorders, including acute or chronic and invasive or non-invasive forms. Invasive fungal sinusitis may develop in an immunocompromised or diabetic patient, whereas non-invasive fungal sinusitis should be considered in a chronic situation, resistant to antibiotics in immunocompetent patients. Allergic fungal sinusitis is related to hypersensitivity of the host to the fungus...
July 10, 2017: Revue des Maladies Respiratoires
https://www.readbyqxmd.com/read/28699277/clinical-evaluation-of-%C3%AE-tubulin-real-time-pcr-for-rapid-diagnosis-of-dermatophytosis-a-comparison-with-mycological-methods
#12
Marjan Motamedi, Hossein Mirhendi, Kamiar Zomorodian, Hossein Khodadadi, Mahboobeh Kharazi, Zeinab Ghasemi, Mohammad Reza Shidfar, Koichi Makimura
Following our previous report on evaluation of the beta tubulin real-time PCR for detection of dermatophytosis, this study aimed to compare the real-time PCR assay with conventional methods for the clinical assessment of its diagnostic performance. Samples from a total of 853 patients with suspected dermatophyte lesions were subjected to direct examination (all samples), culture (499 samples) and real-time PCR (all samples). Fungal DNA was extracted directly from clinical samples using a conical steel bullet, followed by purification with a commercial kit and subjected to the Taq-Man probe-based real-time PCR...
July 12, 2017: Mycoses
https://www.readbyqxmd.com/read/28695189/risk-of-fungal-infection-to-dental-patients
#13
Jaqueline Lopes Damasceno, Rafael Aparecido Dos Santos, Amir Horiquini Barbosa, Luciana Assirati Casemiro, Regina Helena Pires, Carlos Henrique Gomes Martins
Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28688376/molecular-identification-and-antifungal-susceptibility-pattern-of-non-albicans-candida-species-isolated-from-vulvovaginal-candidiasis
#14
Ziba Abbasi Nejat, Shirin Farahyar, Mehraban Falahati, Mahtab Ashrafi Khozani, Aga Fateme Hosseini, Azamsadat Faiazy, Masoome Ekhtiari, Saeideh Hashemi-Hafshenjani
Background: Vulvovaginal candidiasis (VVC) is an important problem due to Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28681818/molecular-identification-and-phenotypic-characterisation-of-sporothrix-globosa-from-clinical-cases-of-eastern-assam-north-east-india
#15
Reema Nath, Pinky Lahon, Longmindar Timung
Sporotrichosis is known to be endemic in the state of Assam, North-east India, which is situated in the Sub-Himalayan region. This disease is an acute or chronic infection caused by Sporothrix schenckii species complex which currently includes several species of clinical relevance such as Sporothrix brasiliensis, Sporothrix globosa, Sporothrix schenckii sensu stricto, Sporothrix albicans, Sporothrix mexicana, Sporothrix pallida and Sporothrix luriei. S. globosa is the prevalent species in India. Eight culture-positive patients were diagnosed from suspected consecutive cases of two lymphocutaneous and six fixed cutaneous forms over a period of 4 years in a clinical mycology laboratory of a tertiary care centre in Eastern Assam...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28681812/evaluation-of-serum-galactomannan-enzyme-immunoassay-at-two-different-cut-offs-for-the-diagnosis-of-invasive-aspergillosis-in-patients-with-febrile-neutropenia
#16
Ritin Mohindra, Malini R Capoor, Shikha Puri, Hitesh Raheja, Dinesh K Gupta, B Gupta, Ranadip Chowdhury
BACKGROUND: Invasive aspergillosis (IA) is an increasingly common and fatal opportunistic fungal infection in patients with haematological diseases. Early diagnosis is difficult as mycological culture techniques have low sensitivity and the radiological tools have low specificity. Galactomannan enzyme immunoassay (GEI) detects galactomannan in the human serum with a reported sensitivity and specificity between 30% and 100%. AIMS: The aim of this study was to analyse the role of GEI in diagnosis of IA in patients with febrile neutropenia and to evaluate the role of GEI in the diagnosis of IA as per the revised (2008) European Organization for Research and Treatment of Cancer-Mycoses Study Group (EORTC-MSG) criteria at two different optical density (OD) cut-offs of 0...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28669831/epidemiology-of-antifungal-resistance-in-human-pathogenic-yeasts-current-viewpoint-and-practical-recommendations-for-management
#17
Dimitrios Farmakiotis, Dimitrios P Kontoyiannis
In this review, we describe the epidemiology and clinical significance of resistance in Candida spp. and other non-Cryptococcus yeasts. The rise in echinocandin resistance, azole resistance and cross-resistance to ≥2 antifungal classes (multi-drug resistance: MDR) has been a worrisome trend, mainly in US large tertiary and oncology centers, particularly as it relates to C. glabrata. C. kefyr is also a concern, as it can be resistant to echinocandins and polyenes, especially in patients with hematologic malignancies...
June 29, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28661360/the-swiss-society-of-microbiology-small-bugs-big-questions-and-cool-answers
#18
Gilbert Greub, Christof Holliger, Dominique Sanglard, Jacques Schrenzel, Volker Thiel, Patrick Viollier
The Swiss Society for Microbiology (SSM) represents around 700 scientists working in the fields of medical (human and veterinary), microbial biotechnology as well as fundamental, environmental, and food microbiology. Five sections: Clinical Microbiology, Environmental Microbiology, Mycology, Prokaryotic Biology, and Virology reflects the main interests of the membership.
December 21, 2016: Chimia
https://www.readbyqxmd.com/read/28646963/spectrum-and-burden-of-dermatophytes-in-children
#19
Leila Ferguson, L Claire Fuller
Tinea capitis is the most important superficial fungal condition in children. It is often unrecognised but the presence of alopecia, scale and lymphadenopathy, especially in inner-city children should prompt investigation. An understanding of changing epidemiology and prevalence of causative organisms is important in deciding appropriate therapy. The use of diagnostic aids including dermoscopy is increasing and is reported to be helpful in identifying subtle signs. Trichophyton tonsurans accounts for the majority of cases in the UK and terbinafine is increasingly advocated as a safe and effective therapy...
June 2017: Journal of Infection
https://www.readbyqxmd.com/read/28627488/diversity-of-veronaea-botryosa-from-different-hosts-and-evaluation-of-laboratory-challenge-models-for-phaeohyphomycosis-in-acipenser-transmontanus
#20
Esteban Soto, Christine Richey, Stephen R Reichley, Brittany Stevens, Kirsten V Kenelty, Janiee Lewis, Barbara Byrne, Nathan P Wiederhold, Thomas B Waltzek, Matthew F Sheley, Alvin C Camus, Matt J Griffin
Veronaea botryosa has been identified as a pathogen of cultured white sturgeon Acipenser transmontanus. In 2015, samples from 19 white sturgeon were received for diagnosis, of which 14 cultured positive for V. botryosa. Intraspecific variability among V. botryosa isolates from different clinically affected hosts and geographic regions was investigated using repetitive extragenic palindromic PCR fingerprinting (rep-PCR). The rep-PCR profiles of 16 V. botryosa isolates from a human, sea turtles, and cultured fish were distinct from those of other phaeoid fungi belonging to the genera Cladophialophora and Exophiala...
June 19, 2017: Diseases of Aquatic Organisms
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