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https://www.readbyqxmd.com/read/29342162/comparison-of-diagnostic-methods-to-detect-histoplasma-capsulatum-in-serum-and-blood-samples-from-aids-patients
#1
Katia Cristina Dantas, Roseli Santos de Freitas, Marcos Vinicius da Silva, Paulo Ricardo Criado, Olinda do Carmo Luiz, Adriana Pardini Vicentini
BACKGROUND: Although early and rapid detection of histoplasmosis is essential to prevent morbidity and mortality, few diagnostic tools are available in resource-limited areas, especially where it is endemic and HIV/AIDS is also epidemic. Thus, we compared conventional and molecular methods to detect Histoplasma capsulatum in sera and blood from HIV/AIDS patients. METHODOLOGY: We collected a total of 40 samples from control volunteers and patients suspected of histoplasmosis, some of whom were also infected with other pathogens...
2018: PloS One
https://www.readbyqxmd.com/read/29325170/ecology-of-paracoccidioides-brasiliensis-p-lutzii-and-related-species-infection-in-armadillos-soil-occurrence-and-mycological-aspects
#2
Marluce Francisca Hrycyk, Hans Garcia Garces, Sandra de Moraes Gimenes Bosco, Sílvio Luis de Oliveira, Sílvio Alencar Marques, Eduardo Bagagli
Paracoccidioides brasiliensis and the related species P. americana, P. restrepiensis, P. venezuelensis, and P. lutzii (Ascomycota, Ajellomycetaceae) are the etiological agents of paracoccidoidoimycosis (PCM), one of the most important systemic mycoses in Latin America. They are dimorphic fungi, with a mycelial life cycle in soil and a yeast phase associated with tissues of mammalian hosts. This study aimed to detect Paracoccidioides spp. in armadillo tissues and associated soil samples in three well-defined geographic areas, including the Alta Floresta, an area not only endemic for PCM in the central region of Brazil but also of probable P...
January 6, 2018: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29323069/recurrent-dermatophytosis-a-rising-problem-in-sikkim-a-himalayan-state-of-india
#3
Rekha Sharma, Luna Adhikari, Rukma Lall Sharma
Changing pattern of dermatophytic infection among people of Sikkim over the past few years and its recurrence rate has brought a need to do a study on clinical pattern and its recurrence from this part of the country. The objectives of this study are to discern the clinical patterns of dermatophytosis, identification of the isolated fungi to its species level and to see the pattern of its recurrence. The study was carried out from January 2015 to May 2016. A total of 192 samples were collected from the patients with clinical findings of dermatophytic infection...
October 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29314679/molecular-epidemiology-of-trichophyton-quinckeanum-a-zoophilic-dermatophyte-on-the-rise
#4
Silke Uhrlaß, Wieland Schroedl, Carolin Mehlhorn, Constanze Krüger, Vit Hubka, Thomas Maier, Yvonne Gräser, Uwe Paasch, Pietro Nenoff
BACKGROUND: Formerly only referred to as a subspecies (T. mentagrophytes var. quinckeanum), T. quinckeanum once again constitutes a distinct species according to the updated taxonomy of dermatophytes. PATIENTS AND METHODS: During routine diagnostic tests conducted at the Mycology Laboratory, Mölbis, Germany, between 11/2013 to 1/2017 (three years and three months), all specimens sent in were examined for T. quinckeanum. Molecular biology methods employed included: 1) DNA hybridization (PCR ELISA), 2) gene sequencing of the ITS region and TEF-1α, and 3) in some cases, MALDI-TOF mass spectrometry...
January 2018: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/29314351/epidemiology-of-dermatophyte-infections-among-school-children-in-menoufia-governorate-egypt
#5
Azza G A Farag, Mostafa A Hammam, Reda A Ibrahim, Reda Z Mahfouz, Nada F Elnaidany, Masroor Qutubuddin, Rehab R E Tolba
BACKGROUND: Most superficial mycotic infections of human skin are due to dermatophytes. Children are frequently affected due to different predisposing factors, particularly overcrowding in classrooms. OBJECTIVES: To estimate the prevalence of dermatophytes infections and their related risk factors among school children in Menoufia Governorate, Egypt. SUBJECTS AND METHODS: Six public primary and preparatory schools were randomly selected and their pupils (n= 3464) were asked to complete a predesigned questionnaire covering both personal data and suspected risk factors for superficial dermatophyte infections...
January 4, 2018: Mycoses
https://www.readbyqxmd.com/read/29306568/mycology-of-chronic-suppurative-otitis-media-cholesteatoma-disease-an-evaluative-study
#6
Gautam Bir Singh, Medozhanuo Solo, Ravinder Kaur, Rubeena Arora, Sunil Kumar
AIMS & OBJECTIVES: To detect the prevalence of fungus in chronic suppurative otitis media-cholesteatoma disease and to evaluate its clinical significance. STUDY DESIGN: Prospective observational study conducted in a sample size of 46 patients at a tertiary care university teaching hospital. MATERIALS & METHODS: Forty six patients suffering from chronic suppurative otitis media-cholesteatoma disease were recruited in this prospective study...
December 2, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/29299825/monitoring-of-ochratoxin-a-and-ochratoxin-producing-fungi-in-traditional-salami-manufactured-in-northern-italy
#7
C Merla, G Andreoli, C Garino, N Vicari, G Tosi, M L Guglielminetti, A Moretti, A Biancardi, M Arlorio, M Fabbi
Fungi have a crucial role in the correct maturation of salami, but special attention should be addressed to the production of the nephrotoxic, immunotoxic, and carcinogenic mycotoxin ochratoxin A (OTA). In a monitoring study conducted in Northern Italy, OTA was detected by liquid chromatography coupled with mass spectrometry in 13 out 133 samples of traditional salami (9.8% of the total count). Mycological analysis of these samples yielded 247 fungal isolates which were identified to species level. The most frequent species were Penicillium nalgiovense, P...
January 3, 2018: Mycotoxin Research
https://www.readbyqxmd.com/read/29285801/tinea-faciei-in-a-central-portuguese-hospital-a-9-year-survey
#8
Ana Borges, Ana Brasileiro, Célia Galhardas, Margarida Apetato
Tinea faciei is a relatively uncommon dermatophytosis that affects the glabrous skin of the face. The aim of this study was to analyse the epidemiologic, clinical and mycological features of tinea faciei cases diagnosed at the Dermatology and Venereology Department of Hospital Santo António dos Capuchos (Lisbon, Portugal). Consecutive cases diagnosed between 2008 and 2016 were studied retrospectively. A total of 72 tinea faciei cases have been diagnosed, involving 37 male and 35 female, aged between 8 months and 86 years...
December 28, 2017: Mycoses
https://www.readbyqxmd.com/read/29281994/successful-treatment-of-cerebral-aspergillosis-case-report-of-a-patient-with-t-cell-large-granular-lymphocytic-leukemia-t-lgl
#9
Amin T Turki, Jassin Rashidi-Alavijeh, Jan Dürig, Guido Gerken, Peter-Michael Rath, Oliver Witzke
BACKGROUND: Invasive aspergillosis involving patients with neutropenia or severe immunosuppression, such as patients with hematologic malignancies is associated with high mortality. Patients with T-cell large granular lymphocytic leukemia (T-LGL) on the other hand are considered to be less vulnerable for severe opportunistic fungal infection as their course of disease is chronic and marked by less violent cytopenia then in e.g. Aplastic Anemia. Only neutropenia is regarded as independent risk factor for severe opportunistic infection in T-LGL patients...
December 28, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29273995/applied-mycology-can-contribute-to-sustainable-rural-livelihoods-building-upon-china-s-matsutake-management-initiatives
#10
Madeline Brown, Timothy McLellan, Huili Li, Samantha C Karunarathna
Matsutake mushrooms are an important part of rural livelihoods and forest ecosystems across large parts of China, as well as elsewhere in East Asia, Northern Europe and North America. Mushroom harvesters have developed sophisticated understandings of matsutake ecology and production, and are applying this knowledge in various innovative management strategies. At the same time, Chinese government agencies and scientists are promoting matsutake-based livelihoods to support development and conservation goals. We collaborated with matsutake harvesters in one Yunnan community to carry out a systematic experiment on a popular shiro-level management technique: covering matsutake shiros with either plastic or leaf litter...
December 22, 2017: Environmental Management
https://www.readbyqxmd.com/read/29261004/exploring-and-exploiting-the-connection-between-mitochondria-and-the-virulence-of-human-pathogenic-fungi
#11
Surbhi Verma, Viplendra P S Shakya, Alexander Idnurm
Mitochondria are best known for their role in the production of ATP; however, recent research implicates other mitochondrial functions in the virulence of human pathogenic fungi. Inhibitors of mitochondrial succinate dehydrogenase or the electron transport chain are successfully used to combat plant pathogenic fungi, but similar inhibition of mitochondrial functions has not been pursued for applications in medical mycology. Advances in understanding mitochondrial function relevant to human pathogenic fungi are in four major directions: 1) the role of mitochondrial morphology in virulence, 2) mitochondrial genetics, with a focus on mitochondrial DNA recombination and mitochondrial inheritance 3) the role of mitochondria in drug resistance, and 4) the interaction of mitochondria with other organelles...
December 20, 2017: Virulence
https://www.readbyqxmd.com/read/29259645/in-vivo-evaluation-of-galla-chinensis-solution-in-the-topical-treatment-of-dermatophytosis
#12
Kai Sun, Xu Song, RenYong Jia, Zhongqiong Yin, Yuanfeng Zou, Lixia Li, Lizi Yin, Changliang He, Xiaoxia Liang, Gang Ye, Guizhou Yue, Xinghong Zhao, Jiankang Yu
Aim: Dermatophytosis is one of the main fungal diseases in humans and animals all over the world. Galla chinensis, a traditional medicine, has various pharmacological effects. The goal of this study was to evaluate the treatment effect of Galla chinensis solution (GCS) on dermatophytosis-infected dogs (Microsporum canis, Microsporum gypseum, and Trichophyton mentagrophytes, resp.). Methods: The treatment effects of GCS were evaluated by mycological cure rates and clinical score comprised of three indices, including inflammation, hair loss, and lesion scale...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29248586/improving-the-diagnosis-of-invasive-aspergillosis-by-the-detection-of-aspergillus-in-broncho-alveolar-lavage-fluid-comparison-of-non-culture-based-assays
#13
Hélène Guegan, Florence Robert-Gangneux, Christophe Camus, Sorya Belaz, Tony Marchand, Marion Baldeyrou, Jean-Pierre Gangneux
OBJECTIVES: This study aimed to evaluate new tools to diagnose invasive aspergillosis (IA) directly from broncho-alveolar lavage (BAL) samples. METHODS: All consecutive patients with suspected IA who underwent bronchoscopy with BAL were prospectively included. Mycological culture and ELISA detection of galactomannan (GM) were performed on BAL. Two in-house and two marketed PCR assays were used on BAL DNA extracts to detect Aspergillus species. Susceptibility testing was performed after culture; marketed PCR assays detected mutations in the CYP51A gene associated to resistance...
December 14, 2017: Journal of Infection
https://www.readbyqxmd.com/read/29242772/fungal-nomenclature-evolving-changes-adopted-by-the-19th-international-botanical-congress-in-shenzhen-2017-and-procedures-for-the-fungal-nomenclature-session-at-the-11th-international-mycological-congress-in-puerto-rico-2018
#14
David L Hawksworth, Tom W May, Scott A Redhead
This article summarizes the key changes in the rules relating to the nomenclature of fungi made at the XIX International Botanical Congress in Shenzhen, China, in July 2017. Most significant was the decision to transfer decision-making on matters related only to the naming of fungi from International Botanical to International Mycological Congresses (IMCs). The rules relating to fungi are to be grouped together in a separate section of the International Code of Nomenclature for algae, fungi, and plants (ICN)...
December 2017: IMA Fungus
https://www.readbyqxmd.com/read/29240863/long-term-follow-up-of-onychomycosis-patients-treated-with-efinaconazole
#15
Richard A Pollak, Carla Ilie
Onychomycosis is a common disease that remains difficult to treat despite the introduction of new topical agents. Clinical trials on efinaconazole and tavaborole included 48 weeks' daily treatment regimens with a 4-week follow-up. It has been suggested that either a longer treatment regimen or longer follow-up would lead to even better results, primarily due to the time taken for the diseased nail to grow out, especially in those patients with more severe disease. <p>OBJECTIVE: To investigate the impact of a longer follow-up period on the efficacy of efinaconazole 10% topical solution in patients with moderate-to-severe onychomycosis...
December 1, 2017: Journal of Drugs in Dermatology: JDD
https://www.readbyqxmd.com/read/29239242/fluconazole-loaded-solid-lipid-nanoparticles-topical-gel-for-treatment-of-pityriasis-versicolor-formulation-and-clinical-study
#16
Shaimaa El-Housiny, Maii Atef Shams Eldeen, Yasmina Ahmed El-Attar, Hoda A Salem, Dalia Attia, Ehab R Bendas, Mohamed A El-Nabarawi
Solid lipid nanoparticles (SLNs) are very potential formulations for topical delivery of antifungal drugs. Hence, the purpose of this research was to formulate the well-known antifungal agent Fluconazole (FLZ)-loaded SLNs topical gel to improve its efficiency for treatment of Pityriasis Versicolor (PV). FLZ-SLNs were prepared by modified high shear homogenization and ultrasonication method using different concentration of solid lipid (Compritol 888 ATO, Precirol ATO5) and surfactant (Cremophor RH40, Poloxamer 407)...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29228380/elderly-versus-nonelderly-patients-with-invasive-aspergillosis-in-the-icu-a-comparison-and-risk-factor-analysis-for-mortality-from-the-aspicu-cohort
#17
D K Matthaiou, G Dimopoulos, F S Taccone, P Bulpa, A M Van den Abeele, B Misset, W Meersseman, H Spapen, T Cardoso, P E Charles, D Vogelaers, S Blot
Data regarding the epidemiology and diagnosis of invasive aspergillosis in the critically ill population are limited, with data regarding elderly patients (≥75 years old) even scarcer. We aimed to further compare the epidemiology, characteristics and outcome of elderly versus nonelderly critically ill patients with invasive aspergillosis (IA) Prospective, international, multicenter observational study (AspICU) including adult intensive care unit (ICU) patients, with a culture and/or direct examination and/or histopathological sample positive for Aspergillus spp...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29228323/evaluation-of-a-genus-specific-elisa-and-a-commercial-aspergillus-western-blot-igg%C3%A2-immunoblot-kit-for-the-diagnosis-of-aspergillosis-in-common-bottlenose-dolphins-tursiops-truncatus
#18
Guillaume Desoubeaux, Carolina Le-Bert, Vanessa Fravel, Tonya Clauss, Alexa J Delaune, Jeny Soto, Eric D Jensen, Jennifer E Flower, Randall Wells, Gregory D Bossart, Carolyn Cray
Aspergillosis is a fungal infection with high mortality and morbidity rates. As in humans, its definitive diagnosis is difficult in animals, and thus new laboratory tools are required to overcome the diagnostic limitations due to low specificity and lack of standardization. In this study of common bottlenose dolphins (Tursiops truncatus), we evaluated the diagnostic performance of a new commercial immunoblot kit that had been initially developed for the serologic diagnosis of chronic aspergillosis in humans...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29216359/fungaemia-caused-by-rare-yeasts-incidence-clinical-characteristics-and-outcome-over-10%C3%A2-years
#19
A Álvarez-Uría, P Muñoz, A Vena, J Guinea, L J Marcos-Zambrano, P Escribano, C Sánchez-Carrillo, E Bouza
No abstract text is available yet for this article.
December 5, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29196924/cutaneotrichosporon-trichosporon-debeurmannianum-a-rare-yeast-isolated-from-blood-and-urine-samples
#20
Reema Nath, Pallabi Sargiary, Biswajyoti Borkakoty, Pratap Parida
Cutaneotrichosporon (Trichosporon) debeurmannianum is a rarely isolated yeast from clinical samples. Nine isolates of this yeast were identified from clinical samples within a period of 3 years from June 2012 to May 2015. These isolates were from blood and urine samples sent to a clinical mycology laboratory of a tertiary care hospital in Assam, North East India. Clinically, the patients were diagnosed as septicemia and urinary tract infection. The age of the patients ranged from 2 to 50 years. Identification was made by sequencing the ITS region of ribosomal RNA gene...
December 1, 2017: Mycopathologia
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