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https://www.readbyqxmd.com/read/28735999/results-of-real-time-multiplex-polymerase-chain-reaction-assay-in-renal-transplant-recipients-with-sterile-pyuria
#1
M Sarier, M Demir, S Goktas, I Duman, M Buyukkinaci, Y Yuksel, S Tekin, A H Yavuz, A Sengul
Urinary tract infections are a major cause of morbidity and hospitalization after renal transplantation. Patients treated with immunosuppressive drugs suffer not only from common uropathogens but also from opportunistic infections caused by unusual uropathogens. Sterile pyuria is associated with numerous infectious agents including viruses, fungi, and atypical or fastidious organisms. The objective of this study was to investigate the pathogens using real-time multiplex polymerase chain reaction (rtMPCR) assay in sterile pyuria of renal transplant recipients...
July 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28730647/swimming-pools-and-fungi-an-epidemiology-survey-in-polish-indoor-swimming-facilities
#2
Marek Jankowski, Anna Charemska, Rafał Czajkowski
Environmental conditions for swimming facilities may support fungal growth and also may represent a biological risk for users. However, since previously published studies both cleaning procedures and sanitary regulations are likely to have been improved. The aim of the study was to examine whether attendance to public swimming pools was still a risk factor for fungal infection of the skin in Poland. We investigated the occurrence of mycotic species, in a sample from Polish swimming pools. Detection and identification of isolated species were achieved by cultural and morphological methods...
July 20, 2017: Mycoses
https://www.readbyqxmd.com/read/28730378/enzymatic-activity-of-candida-spp-from-oral-cavity-and-urine-in-children-with-nephrotic-syndrome
#3
Dorota Olczak-Kowalczyk, Maria Roszkowska-Blaim, Maria Dąbkowska, Ewa Swoboda-Kopeć, Dariusz Gozdowski, Małgorzata Mizerska-Wasiak, Urszula Demkow, Małgorzata Pańczyk-Tomaszewska
Oral colonization with Candida spp. is not synonymous with a systemic active infection. The aim of the study was to evaluate enzymatic activity of Candida strains isolated from the oral cavity in patients with nephrotic syndrome (NS) and to compare it with the activity determined in urine. We studied 32 children with NS and 26 control healthy children. Children with NS were treated with glucocorticosteroids, cyclosporin A, mycophenolate mofetil or azathioprine. In all children, API-ZYM enzymatic tests were performed to evaluate hydrolytic enzymes of Candida isolated from the oral cavity and in urine...
July 21, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28729856/biological-significance-of-marine-actinobacteria-of-east-coast-of-andhra-pradesh-india
#4
Alapati Kavitha, Handanahal S Savithri
An attempt was made to identify actinobacterial strains present in the marine soil of East Coast regions viz., Chirala, Bapatla, and Peddaganjam, Andhra Pradesh; Kanyakumari, Tamil Nadu and Goa, Goa along with the study of their antimicrobial potential. Eight out of 73 actinobacterial strains isolated from these regions showed strong antimicrobial activity against Gram positive bacteria, Gram negative bacteria, and Candida albicans. Molecular identification (16S rRNA analysis) of the eight strains revealed that they belong to Dietzia sp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28728202/-pyogenic-liver-abscess-changes-of-organisms-and-consequences-for-diagnosis-and-therapy
#5
Alexander Mischnik, Winfried Vincenz Kern, Robert Thimme
Liver abscess is a rare but potentially fatal disease with mortality rates of 2 - 12 %. The spectrum of causative microorganisms varies across the world and is changing due to increasing worldwide travel activity. As a result, previously less frequent pathogens must increasingly be considered in the differential diagnosis. The most common pathogens of liver abscess are so-called abscess streptococci, anaerobes and Gram-negative bacteria such as Escherichia coli and Klebsiella pneumoniae. Certain clones among the Gram-negatives, typically among Klebsiella spp...
July 2017: Deutsche Medizinische Wochenschrift
https://www.readbyqxmd.com/read/28720316/pigeons-and-their-droppings-as-reservoirs-of-candida-and-other-zoonotic-yeasts
#6
Inmaculada Rosario Medina, Lorena Román Fuentes, Miguel Batista Arteaga, Fernando Real Valcárcel, Félix Acosta Arbelo, Daniel Padilla Del Castillo, Soraya Déniz Suárez, Otilia Ferrer Quintana, Belinda Vega Gutiérrez, Freddy Silva Sergent, Begoña Acosta-Hernández
BACKGROUND: The importance of pigeons as reservoirs and carriers of Cryptococcus neoformans and other species of this genus is well-known; however, less is known about their role as reservoirs and carriers of other yeasts that impact public health. AIMS: The present study was performed on Gran Canaria Island to define yeasts other than Cryptococcus spp. that have been reported to impact public health and which could be carried by pigeons. METHODS: Samples were obtained from 83 pigeon lofts (Columba livia); moreover, 331 crop samples, 331 cloacal samples and 174 dropping samples were collected...
July 15, 2017: Revista Iberoamericana de Micología
https://www.readbyqxmd.com/read/28711430/implementation-of-a-national-bundle-care-program-to-reduce-catheter-associated-urinary-tract-infection-in-high-risk-units-of-hospitals-in-taiwan
#7
Chih-Cheng Lai, Chun-Ming Lee, Hsiu-Tzy Chiang, Ching-Tzu Hung, Ying-Chun Chen, Li-Hsiang Su, Zhi-Yuan Shi, Jein-Wei Liu, Chang-Pan Liu, Min-Chi Lu, Yin-Ching Chuang, Wen-Chien Ko, Shu-Hui Tseng, Yen-Hsu Chen, Po-Ren Hsueh
BACKGROUND/PURPOSE: This study was intended to investigate the impact of implementation of catheter-associated urinary tract infection (CA-UTI) bundle care on the incidence of CA-UTI in high-risk units. METHODS: Thirteen high-risk units, including medical (n = 5), surgical (n = 3), cardiac intensive care units (n = 2), respiratory care centers (n = 2), and respiratory care ward (n = 1) were included in this quality-improvement project. This study was divided into pre-intervention phase (from January 1 to July 31) and post-intervention phase (from August 1 to October 31) in 2013...
June 26, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28704470/mammary-candidiasis-a-medical-condition-without-scientific-evidence
#8
Esther Jiménez, Rebeca Arroyo, Nivia Cárdenas, María Marín, Pilar Serrano, Leonides Fernández, Juan M Rodríguez
Many physicians, midwives and lactation consultants still believe that yeasts (particularly Candida spp.) play an important role as an agent of nipple and breast pain despite the absolute absence of scientific proofs to establish such association. In this context, the objective of this study was to investigate the microorganisms involved in sore nipples and/or painful "shooting" breastfeeding by using a variety of microscopy techniques, as well as culture-dependent and-independent identification methods. Initially, 60 women (30 diagnosed as suffering "mammary candidiasis" and 30 with no painful breastfeeding) were recruited to elucidate the role of their pumps on the milk microbial profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28703342/evaluation-of-direct-antifungal-susceptibility-testing-methods-of-candida-spp-from-positive-blood-culture-bottles
#9
Yasemin Oz, Egemen Gokbolat
BACKGROUND: Blood culture is the gold standard diagnostic method in candidemia in spite of long result time and low sensitivity rate. Early diagnosis is crucial for management of candidemia because a delay in treatment is related with increased mortality. We aimed to evaluate the direct applicability of antifungal susceptibility testing methods from positive blood culture bottles to save at least 24 hours. METHODS: Blood culture bottles were inoculated with 62 Candida isolates...
July 13, 2017: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/28703118/cholelithiasis-always-infected
#10
Tomasz Matyjas, Krzysztof Kaczka, Henryk Witas, Tomasz Płoszaj, Katarzyna Matyjas, Lech Pomorski
This study aims to present results regarding the presence and identification of bacterial strains found in bile and gallstones located in the gallbladder and bile ducts in patients operated on due to cholelithiasis. MATERIALS AND METHODS: Bacterial culture was evaluated in 92 patients. There were 54 women (59%) and 38 men (41%) who underwent surgery on account of cholelithiasis and /or gallstones in bile ducts between 2013 and 2014. Bile and gallstone samples were cultured intraoperatively for bacteria; bacterial strains were identified, and their sensitivity to antibiotics was determined...
June 30, 2017: Polski Przeglad Chirurgiczny
https://www.readbyqxmd.com/read/28702025/cellular-and-molecular-defects-underlying-invasive-fungal-infections-revelations-from-endemic-mycoses
#11
REVIEW
Pamela P Lee, Yu-Lung Lau
The global burden of fungal diseases has been increasing, as a result of the expanding number of susceptible individuals including people living with human immunodeficiency virus (HIV), hematopoietic stem cell or organ transplant recipients, patients with malignancies or immunological conditions receiving immunosuppressive treatment, premature neonates, and the elderly. Opportunistic fungal pathogens such as Aspergillus, Candida, Cryptococcus, Rhizopus, and Pneumocystis jiroveci are distributed worldwide and constitute the majority of invasive fungal infections (IFIs)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28699287/an-alternative-method-for-the-analysis-of-melanin-production-in-cryptococcus-neoformans-sensu-lato-and-cryptococcus-gattii-sensu-lato
#12
Raimunda S N Brilhante, Jaime D A España, Lucas P de Alencar, Vandbergue S Pereira, Débora de S C M Castelo-Branco, Waldemiro de A Pereira-Neto, Rossana de A Cordeiro, José J C Sidrim, Marcos F G Rocha
Melanin is an important virulence factor for several microorganisms, including Cryptococcus neoformans sensu lato and Cryptococcus gattii sensu lato, thus, the assessment of melanin production and its quantification may contribute to the understanding of microbial pathogenesis. The objective of this study was to standardise an alternative method for the production and indirect quantification of melanin in C. neoformans sensu lato and C. gattii sensu lato. Eight C. neoformans sensu lato and three C. gattii sensu lato, identified through URA5 methodology, Candida parapsilosis ATCC 22019 (negative control) and one Hortaea werneckii (positive control) were inoculated on minimal medium agar with or without L-DOPA, in duplicate, and incubated at 35°C, for 7 days...
July 12, 2017: Mycoses
https://www.readbyqxmd.com/read/28695189/risk-of-fungal-infection-to-dental-patients
#13
Jaqueline Lopes Damasceno, Rafael Aparecido Dos Santos, Amir Horiquini Barbosa, Luciana Assirati Casemiro, Regina Helena Pires, Carlos Henrique Gomes Martins
Fungi can cause various diseases, and some pathogenic fungi have been detected in the water of dental equipment. This environment offers suitable conditions for fungal biofilms to emerge, which can facilitate mycological contamination. This study verified whether the water employed in the dental units of two dental clinics at the University of Franca was contaminated with fungi. This study also evaluated the ability of the detected fungi to form biofilms. The high-revving engine contained the largest average amount of fungi, 14...
2017: TheScientificWorldJournal
https://www.readbyqxmd.com/read/28694609/role-of-biofilm-in-cerebrospinal-fluid-shunt-infections-a-study-at-tertiary-neurocare-center-from-south-india
#14
Kirtilaxmi K Benachinmardi, R Ravikumar, B Indiradevi
INTRODUCTION: Biofilms are the source of persistent infections of many pathogenic microbes. They are responsible for nosocomial infection and also associated with many surgical conditions including indwelling medical devices such as ventriculoperitoneal shunt. A significant problem encountered in shunt procedures is obstruction followed by infection, with infection rate ranging from 2% to 27%, often with poor outcome. MATERIALS AND METHODS: This study was conducted in the Department of Neuromicrobiology at a tertiary neuroinstitute for 6 months from July 1 to December 31, 2014...
July 2017: Journal of Neurosciences in Rural Practice
https://www.readbyqxmd.com/read/28689871/cd101-a-long-acting-echinocandin-and-comparator-antifungal-agents-tested-against-a-global-collection-of-invasive-fungal-isolates-in-the-sentry-2015-antifungal-surveillance-program
#15
Michael A Pfaller, Shawn A Messer, Paul R Rhomberg, Mariana Castanheira
CD101 is a novel echinocandin with exceptional chemical stability and long-acting pharmacokinetics. The activity of CD101 and comparators was evaluated using CLSI broth microdilution methods against 713 invasive fungal isolates, including 589 Candida spp. (6 species), 14 C. neoformans, 97 A. fumigatus and 13 A. flavus species complex collected worldwide during 2015. All C. tropicalis, C. krusei and C. dubliniensis, 99.7% of C. albicans and 98.3% of C. glabrata were inhibited by ≤0.12 µg/mL of CD101, and these isolates were susceptible/wild type to other echinocandins using CLSI clinical breakpoint and epidemiological cutoff value (ECV) interpretive criteria...
July 6, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28688376/molecular-identification-and-antifungal-susceptibility-pattern-of-non-albicans-candida-species-isolated-from-vulvovaginal-candidiasis
#16
Ziba Abbasi Nejat, Shirin Farahyar, Mehraban Falahati, Mahtab Ashrafi Khozani, Aga Fateme Hosseini, Azamsadat Faiazy, Masoome Ekhtiari, Saeideh Hashemi-Hafshenjani
Background: Vulvovaginal candidiasis (VVC) is an important problem due to Candida spp. The aim of this study was molecular identification, phylogenetic analysis, and evaluation of antifungal susceptibility of non-albicans Candida isolates from VVC. Methods: Vaginal secretion samples were collected from 550 vaginitis patients at Sayyad Shirazi Medical and Educational Center of Gorgan (Golestan Province, Iran) from May to October 2015. Samples were analyzed using conventional mycological and molecular approaches...
July 9, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28685557/the-use-of-chlorhexidine-in-mouthguards
#17
S D Ercole, M Tieri, D Fulco, D Martinelli, D Tripodi
Sports mouthguards have the potential to become a microbial reservoir, to produce oral and systemic diseases and cause changes in environmental oral factors, inhibiting the protective effect of saliva. The aim of this study was to monitor, in vivo, oral environmental changes caused by chlorhexidine and sports mouthguards and in vitro, the effects of microbial strains, saliva and chlorhexidine on Ethylene-Vinyl-Acetate (EVA) material. Seventy-two athletes were analyzed at different time points: before training session (T0), post-training (TA), post-training with mouthguard (TB), post-training with mouthguard and chlorhexidine (TC)...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28681815/identification-of-the-paradoxical-effect-of-caspofungin-and-micafungin-on-candida-albicans-growth-in-vitro-using-a-growth-curve-methodology
#18
Huang Zhouying, Li Chen, Wang Ruiqi, Liao Kang, Zeng Ji
BACKGROUND: The paradoxical effect of fungi, (which is a special phenomenon that certain Candida spp. might exhibit augmented growth in the presence of echinocandin at some concentration higher than the minimum inhibitory concentration) would be better illustrated through growth curves. MATERIALS AND METHODS: We investigated the paradoxical effect of caspofungin and micafungin on the growth and cell viability of 38 clinical isolates of Candida albicans using a microbial sensitivity test and growth curve methodology...
April 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28676673/formulation-and-candidacidal-activity-of-magnetic-nanoparticles-coated-with-cathelicidin-ll-37-and-ceragenin-csa-13
#19
Katarzyna Niemirowicz, Bonita Durnaś, Grażyna Tokajuk, Ewelina Piktel, Grzegorz Michalak, Xiaobo Gu, Alina Kułakowska, Paul B Savage, Robert Bucki
Fungal infections caused by Candida spp. represent an emerging problem during treatment of immunocompromised patients and those hospitalized with serious principal diseases. The ever-growing number of fungal strains exhibiting drug resistance necessitates the development of novel antimicrobial therapies including those based on membrane-permeabilizing agents and nanomaterials as drug carriers. In this study, the fungicidal activities of LL-37 peptide, ceragenin CSA-13 and its magnetic derivatives (MNP@LL-37, MNP@CSA-13) against laboratory and clinical strains of C...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28669835/molecular-basis-of-antifungal-drug-resistance-in-yeasts
#20
Florent Morio, Rasmus Hare Jensen, Patrice Le Pape, Maiken Cavling Arendrup
Besides inherent differences in in vitro susceptibilities, clinically relevant yeast species may acquire resistance upon exposure to most antifungal drugs used in the clinic. In recent years, major fundamental research studies have been conducted to improve our understanding of the molecular basis of antifungal resistance. This topic is of major interest as antifungal resistance in yeast is correlated to clinical failure and is clearly evolving. In this minireview, we provide an overview including the most recent findings about key molecular mechanisms evolving in human pathogenic yeasts, especially Candida spp...
June 29, 2017: International Journal of Antimicrobial Agents
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