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https://www.readbyqxmd.com/read/28329720/in-vitro-activity-of-farnesol-against-vaginal-lactobacillus-spp
#1
Fengjuan Wang, Zhaohui Liu, Dai Zhang, Xiaoxi Niu
OBJECTIVE: Farnesol, a quorum-sensing molecule in Candida albicans, can affect the growth of certain microorganisms. The objective of this study was to evaluate the in vitro activity of farnesol against vaginal Lactobacillus spp., which play a crucial role in the maintenance of vaginal health. METHODS: Growth and metabolic viability of vaginal Lactobacillus spp. incubated with different concentrations of farnesol were determined by measuring the optical density of the cultures and with the MTT assay...
March 7, 2017: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/28327806/invasive-fungal-infection-in-patients-with-hematologic-disorders-in-a-brazilian-tertiary-care-hospital
#2
Milton Camplesi, Hildene Meneses Silva, Adriano Moraes Arantes, Carolina Rodrigues Costa, Fábio Silvestre Ataides, Thaisa Cristina Silva, Maysa de Paula Costa Dos Reis, Maria do Rosário Rodrigues Silva
INTRODUCTION: Invasive fungal infections (IFIs) are an important complication in immunocompromised individuals, particularly neutropenic patients with hematological malignancies. In this study, we aimed to verify the epidemiology and diagnosis of IFIs in patients with hematologic problems at a tertiary hospital in Goiânia-GO, Brazil. METHODS: Data from 117 patients, involving 19 cases of IFIs, were collected. The collected data included diagnosis methods, demographics, clinical characteristics, and in vitro susceptibility to different antifungal agents...
January 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28327264/association-of-candida-species-isolated-from-the-dental-plaque-of-hiv-infected-children-and-prevalence-of-early-carious-lesions
#3
Cristiana Aroeira Rosa Oliveira, Senda Charone, Rosangela Maria de Araújo Soares, Maristela Barbosa Portela, Gloria Fernanda Barbosa de Aráujo Castro
PURPOSE: The purposes of this study were to: (1) quantify and identify Candida species (spp.) in the dental plaque of children infected with the human immunodeficiency virus (HIV) and compare with noninfected children; and (2) determine the association of Candida spp. with the presence of caries. METHODS: Seventy HIV-infected and 55 non-HIV-infected three- to 12-year-old children were examined to determine caries prevalence. After a visual inspection, supragingival plaque was collected from the cervical region using standard dental curettes...
September 15, 2016: Journal of Dentistry for Children
https://www.readbyqxmd.com/read/28315730/synthesis-antimicrobial-activity-and-toxicity-to-nematodes-of-cyclam-derivatives
#4
Luis G Alves, Pedro F Pinheiro, Joana R Feliciano, Diana P Dâmaso, Jorge H Leitão, Ana M Martins
The antimicrobial activity and toxicity to nematodes of the cyclam salt [H2{H2((4-CF)3PhCH2)2Cyclam}](CH3COO)2.(CH3COOH)2 was evaluated. Estimated minimum inhibitory concentrations (MICs) of 9, 261 and 15 µg/mL were obtained for Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus, respectively. For selected Candida spp., the estimated MICs obtained ranged from 32 µg/mL to 63 µg/mL. Bactericidal activity was demonstrated but the compound was not reliably fungicidal. Concentrations of the cyclam salt up to 32 µg/mL did not significantly affect survival of the nematode Caenorhabditis elegans; however, concentrations equal or above this value significantly affected nematode survival in a dose-dependent manner...
March 15, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28303122/antifungal-drug-susceptibility-of-candida-species-isolated-from-hiv-positive-patients-recruited-at-a-public-hospital-in-s%C3%A3-o-lu%C3%A3-s-maranh%C3%A3-o-brazil
#5
Ana L G Terças, Sirlei G Marques, Eduardo B Moffa, Márcia B Alves, Conceição M P S de Azevedo, Walter L Siqueira, Cristina A Monteiro
Oropharyngeal candidiasis is the most common fungal infection in hospitalized patients with acquired immune deficiency syndrome (AIDS). Its progression results in invasive infections, which are a significant cause of morbidity and mortality. This study aimed to quickly and accurately identify Candida spp. from oral mucosa of AIDS patients recruited at Presidente Vargas Hospital, in São Luís city, Brazil and to evaluate the sensitivity profile of these fungi to antifungals by using an automated system. Isolates were collected from oropharyngeal mucosa of 52 hospitalized AIDS patients, under anti-viral and antifungal therapies...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28302347/fungemia-due-to-saprochaete-capitata-in-a-non-neutropenic-patient-hospitalized-in-an-intensive-care-unit-after-cardiac-surgery
#6
C Cavanna, F Lallitto, F Mangione, F Tamarozzi, P Marone, P Ceriana
The majority of invasive fungal infections observed in non-neutropenic patients hospitalized in an intensive care unit are caused by Candida spp and current guidelines recommend echinocandins as the first-line treatment. Fungemias caused by filamentous or arthrosporic fungi such as Saprochaete capitata (previously named Geotrichum capitatum) are extremely rare. In fact, invasive infections due to S. capitata have been reported almost exclusively in neutropenic oncohematological patients. In this report, we describe a case of fungemia caused by S...
March 14, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28290221/microbiological-diagnosis-and-antimicrobial-sensitivity-profiles-in-diseased-free-living-raptors
#7
Anna Vidal, Laia Baldomà, Rafael A Molina-Lopez, Marga Martin, Laila Darwich
Free-living raptors (birds of prey) can act as reservoirs of potentially zoonotic agents but they also can be affected by microorganisms as target hosts. In this retrospective study, microbiological results (n=663) and antibiotic sensitivity profiles (n=108) of bacterial isolates were analyzed from diseased free-living raptors. Sixty-nine percent of cases (n=457) yielded bacteria: 58% were in pure culture and 42% were of different species. Remarkably, samples from necropsies (47%) had higher percentage of pure isolations than those obtained from clinical (31%) samples (p<0...
March 14, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28267809/lactobacillus-paracasei-modulates-the-immune-system-of-galleria-mellonella-and-protects-against-candida-albicans-infection
#8
Rodnei Dennis Rossoni, Beth Burgwyn Fuchs, Patrícia Pimentel de Barros, Marisol Dos Santos Velloso, Antonio Olavo Cardoso Jorge, Juliana Campos Junqueira, Eleftherios Mylonakis
Probiotics have been described as a potential strategy to control opportunistic infections due to their ability to stimulate the immune system. Using the non-vertebrate model host Galleria mellonella, we evaluated whether clinical isolates of Lactobacillus spp. are able to provide protection against Candida albicans infection. Among different strains of Lactobacillus paracasei, Lactobacillus rhamnosus and Lactobacillus fermentum, we verified that L. paracasei 28.4 strain had the greatest ability to prolong the survival of larvae infected with a lethal dose of C...
2017: PloS One
https://www.readbyqxmd.com/read/28263903/candida-and-its-dual-lifestyle-as-a-commensal-and-a-pathogen
#9
Mathieu Cauchie, Stefanie Desmet, Katrien Lagrou
Candida spp. are part of the normal vaginal microflora in 20-30% of healthy women. However, if the balance between these yeasts and the host is disturbed, Candida spp. can cause vulvovaginal candidiasis (VVC), with Candida albicans being the major causative agent. Different studies have been performed in order to better understand Candida's dual lifestyle in the vagina. The potential of C. albicans to switch from the yeast cell morphology to its hyphal form is considered a key element in VVC pathogenesis. Candida spp...
March 2, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28260677/-au-py-b-h-mnt-a-novel-gold-iii-1-2-dithiolene-cyclometalated-complex-with-antimicrobial-activity-py-b-h-c-deprotonated-2-benzylpyridine-mnt-1-2-dicyanoethene-1-2-dithiolate
#10
Anna Pintus, M Carla Aragoni, Maria A Cinellu, Laura Maiore, Francesco Isaia, Vito Lippolis, Germano Orrù, Enrica Tuveri, Antonio Zucca, Massimiliano Arca
The novel heteroleptic cyclometalated complex [Au(III)(py(b)-H)(mnt)] (1; py(b)-H=C-deprotonated 2-benzylpyridine; mnt =1,2-dicyanoethene-1,2-dithiolate) was tested against a panel of ten Gram positive (belonging to the Staphylococcus, Streptococcus spp. and Bacillus clausii), Gram negative (E. coli, K. pneumoniae, P. aeruginosa) bacteria and three yeasts belonging to the Candida spp. Complex 1 showed a remarkable bacteriostatic antimicrobial activity against staphylococci, with Minimum Inhibitory Concentration (MIC) values of 1...
May 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28259673/unexplored-endemic-fruit-species-from-brazil-antibiofilm-properties-insights-into-mode-of-action-and-systemic-toxicity-of-four-eugenia-spp
#11
Janaina de Cássia Orlandi Sardi, Irlan Almeida Freires, Josy Goldoni Lazarini, Juliana Infante, Severino Matias de Alencar, Pedro Luiz Rosalen
Brazilian endemic fruit species have aroused attention due to their highly valuable, yet unexplored, agro-industrial, food and therapeutic potential. Herein, we describe the antifungal activity of four Eugenia spp. against Candida albicans biofilms, and further demonstrate insights into their potential mode(s) of action and toxicity in vitro and in vivo. Extracts from different parts (seeds, pulps, leaves) of E. leitonii (EL), E. brasiliensis (EB), E. myrcianthes (EM) and E. involucrata (EI) were obtained (S23°23',W45°39') and chemically characterized by GC/MS...
March 1, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28255766/the-colonization-with-candida-species-is-more-harmful-in-the-second-trimester-of-pregnancy
#12
Iris Holzer, Alex Farr, Herbert Kiss, Michael Hagmann, Ljubomir Petricevic
PURPOSE: Vaginal colonization with Candida species (spp.) during pregnancy has been associated with impaired pregnancy outcomes. There is a reduction in spontaneous preterm birth among women with recurrent asymptomatic colonization of Candida who were treated with clotrimazole. This study aimed to evaluate the impact of the trimester of vulvovaginal colonization with Candida species. METHODS: Data from all women, who were tested positive for the vaginal colonization with Candida spp...
March 3, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28255613/intensive-care-medicine-research-agenda-on-invasive-fungal-infection-in-critically-ill-patients
#13
REVIEW
Matteo Bassetti, Jose Garnacho-Montero, Thierry Calandra, Bartjan Kullberg, George Dimopoulos, Elie Azoulay, Arunaloke Chakrabarti, Daniel Kett, Cristobal Leon, Luis Ostrosky-Zeichner, Maurizio Sanguinetti, Jean-Francois Timsit, Malcom D Richardson, Andrew Shorr, Oliver A Cornely
PURPOSE: To describe concisely the current standards of care, major recent advances, common beliefs that have been contradicted by recent trials, areas of uncertainty, and clinical studies that need to be performed over the next decade and their expected outcomes with regard to Candida and Aspergillus infections in non-neutropenic patients in the ICU setting. METHODS: A systematic review of the medical literature taking account of national and international guidelines and expert opinion...
March 2, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28250661/antifungal-activity-of-hydroalcoholic-extract-of-chrysobalanus-icaco-against-oral-clinical-isolates-of-candida-species
#14
João Paulo Bastos Silva, Ana Regina Maués Noronha Peres, Thiago Portal Paixão, Andressa Santa Brígida Silva, Ana Cristina Baetas, Wagner Luiz Ramos Barbosa, Marta Chagas Monteiro, Marcieni Ataíde Andrade
BACKGROUND: Chrysobalanus icaco is a medicinal plant commonly used to treat fungal infections in Brazilian Amazonian region. OBJECTIVE: This work aimed to evaluate the antifungal activity of the hydroalcoholic extract of C. icaco (HECi) against oral clinical isolates of Candida spp. and to determine the pharmacognostic parameters of the herbal drug and the phytochemical characteristics of HECi. MATERIALS AND METHODS: The pharmacognostic characterization was performed using pharmacopoeial techniques...
January 2017: Pharmacognosy Research
https://www.readbyqxmd.com/read/28250619/studying-the-prevalence-species-distribution-and-detection-of-in-vitro-production-of-phospholipase-from-candida-isolated-from-cases-of-invasive-candidiasis
#15
Yukti Sharma, Susheel Kumar Chumber, Mandeep Kaur
BACKGROUND AND AIM: Candida spp. have emerged as successful pathogens both in invasive and mucosal infections. C. albicans is the sixth cause of most common nosocomial infections according to studies by the Centers for Disease Control and Prevention. A shift toward non-albicans species has been reported. There is a dearth of knowledge regarding the virulence factors of Candida, especially from this part of India. The aim was to study the prevalence of Candida, speciate, and determine antifungal sensitivity along with the detection of in vitro production of phospholipases in 100 Candida isolates...
January 2017: Journal of Global Infectious Diseases
https://www.readbyqxmd.com/read/28247627/-five-year-analysis-of-microbial-landscape-of-transplantation-and-dialysis-center-20112015
#16
A B Zulkarnaev, A V Vatazin, E V Rusanova, V A Fedulkina, S A Pasov
INTRODUCTION: Treatment of bacterial infections is pressing and difficult task of modern transplantology. AIM: To analyze the spectrum of bacterial microflora and bacterial sensitivity to antibiotics in patients of the transplantation and dialysis center. RESULTS: The study analyzed the results of bacteriological tests performed in 534 patients with chronic kidney disease stage 5 in 2011-2015. Biomaterials for the examination included urine, blood, wound discharge and sputum...
August 2016: Urologii︠a︡
https://www.readbyqxmd.com/read/28238268/aryl-alkyl-lysines-membrane-active-fungicides-that-act-against-biofilms-of-candida-albicans
#17
Chandradhish Ghosh, Vikas Yadav, Waleed Younis, Haroon Mohammad, Youssef A Hegazy, Mohamed N Seleem, Kaustuv Sanyal, Jayanta Haldar
Mortality due to pathogenic fungi has been exacerbated by the rapid development of resistance to frontline antifungal drugs. Fungicidal compounds with novel mechanisms of action are urgently needed. Aryl-alkyl-lysines, which are membrane-active small molecules, were earlier shown to be broad-spectrum antibacterial agents with potency in vitro and in vivo. Herein, we report the antifungal properties of aryl-alkyl-lysines. After identifying the most active compound (NCK-10), we tested its activity against a panel of clinically relevant pathogenic fungi and examined NCK-10's effect against immature and mature biofilms of Candida albicans...
March 14, 2017: ACS Infectious Diseases
https://www.readbyqxmd.com/read/28229205/-spectrum-of-pathogens-in-postoperative-complications-of-visceral-surgery-the-problem-of-multidrug-resistance
#18
A Kramer, J Pochhammer, P Walger, U Seifert, M Ruhnke, J C Harnoss
In general surgery the etiology of surgical site infections has not significantly changed over the last 30 years. Gram-positive bacteria, e.g. coagulase negative staphylococci (CNS), Staphylococcus aureus and Enterococcus spp. as well as Gram-negative bacteria, e.g. Escherichia coli, Enterobacter spp., Klebsiella spp. and Pseudomonas aeruginosa, are the most common findings. Although in general surgery 10% of the S. aureus causing postoperative wound infections were methicillin resistant (MRSA), no cases of multidrug-resistant Gram-negative (MRGN) bacteria were reported...
February 22, 2017: Der Chirurg; Zeitschrift Für Alle Gebiete der Operativen Medizen
https://www.readbyqxmd.com/read/28225185/preventive-effects-of-topical-washing-with-miconazole-nitrate-containing-soap-to-diaper-candidiasis-in-hospitalized-elderly-patients-a-prospective-double-blind-placebo-controlled-study
#19
Hidenori Takahashi, Noritaka Oyama, Izumi Tanaka, Michiko Hasegawa, Kaori Hirano, Chieko Shimada, Minoru Hasegawa
The objective of the present randomized, double-blind trial was to evaluate the efficacy and safety of daily washing with miconazole nitrate-containing soap for candidiasis at diaper-covered sites in elderly subjects under long-term inpatient care. To confirm the onset and disappearance of candidiasis, we microscopically evaluated the existence of the pseudohyphae and/or blastoconidia of Candida spp. We enrolled 75 elderly patients who wore diapers all day in the hospital or nursing home. Patients were randomly assigned to receive treatment with either miconazole soap or miconazole-free placebo soap, and 28 patients in the miconazole group and 27 patients in the placebo group were followed for 4 weeks...
February 22, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28222388/microbial-profile-of-dental-plaque-associated-to-white-spot-lesions-in-orthodontic-patients-immediately-after-the-bracket-removal
#20
Moniek W Beerens, Jacob M Ten Cate, Monique H van der Veen
OBJECTIVE: Denaturing Gradient Gel Electrophoresis (DGGE) is suggested to predict caries risk in young children. Such a tool would be valuable in orthodontic patients undergoing treatment with fixed appliances. In this cross-sectional study the applicability of DGGE and conventional microbiology for caries risk assessment in orthodontic patients were assessed. DESIGN: Dental plaque was obtained from orthodontic patients immediately prior to bracket removal. Presence of white spot lesions (WSL) was assessed immediately post debracketing...
February 9, 2017: Archives of Oral Biology
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