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https://www.readbyqxmd.com/read/28646812/oral-consumption-of-vitamin-k2-for-8-weeks-associated-with-increased-maximal-cardiac-output-during-exercise
#1
Brian K McFarlin, Andrea L Henning, Adam S Venable
Background • Vitamin K1 and K2 are not typically common in a Western diet because they are found in a variety of fermented foods. Vitamin K2 in particular has been demonstrated to restore mitochondrial function and has a key role in production of mitochondrial adenosine triphosphate. Thus, it is reasonable to speculate that dietary supplementation with vitamin K2 could increase the function of muscle with high mitochondrial content (ie, skeletal and cardiac muscle). Objective • The purpose of this study was to determine if 8 wk of dietary supplementation with Vitamin K2 could alter cardiovascular responses to a graded cycle ergometer test...
July 2017: Alternative Therapies in Health and Medicine
https://www.readbyqxmd.com/read/28646798/social-context-matters-ethnicity-discrimination-and-stress-reactivity
#2
David Busse, Ilona S Yim, Belinda Campos
Exposure to chronic discrimination is associated with increased morbidity and mortality. The study of biobehavioral pathways linking discrimination with health outcomes has mostly focused on the cardiovascular system, with fewer studies addressing the hypothalamus-pituitary-adrenal (HPA) axis. In this study we tested associations between Latino ethnicity, experiences of discrimination, and cortisol responses to an acute laboratory stressor. One hundred fifty eight individuals (92 female, 66 male) between the ages of 18 and 29 years participated in the study...
May 31, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28646766/gaps-in-aquatic-toxicological-studies-of-microplastics
#3
REVIEW
Ali Karami
The contamination of aquatic environments with microplastics (MPs) has spurred an unprecedented interest among scientific communities to investigate their impacts on biota. Despite the rapid growth in the number of studies on the aquatic toxicology of MPs, controversy over the fate and biological impacts of MPs is increasingly growing mainly due to the absence of standardized laboratory bioassays. Given the complex features of MPs, such as the diversity of constituent polymers, additives, shapes and sizes, as well as continuous changes in the particle buoyancy as a result of fouling and defouling processes, it is necessary to modify conventional bioassay protocols before employing them for MP toxicity testings...
June 14, 2017: Chemosphere
https://www.readbyqxmd.com/read/28646669/validation-of-a-commercial-kit-aimed-to-the-detection-of-pathogenic-anisakid-nematodes-in-fish-products
#4
Serena Cavallero, Alessandro Bruno, Enrico Arletti, Monica Caffara, Maria Letizia Fioravanti, Antonella Costa, Gaetano Cammilleri, Stefania Graci, Vincenzo Ferrantelli, Stefano D'Amelio
Anisakids are parasitic nematodes responsible for a zoonosis that occurs following the ingestion of fish and fish products infected with larvae belonging to the genera Anisakis and Pseudoterranova. Rarely Contracaecum is found in association with gastric/intestinal illness, while Hysterothylacium is commonly considered not pathogenic. Although Real Time PCR assays have been recently used with the aim to detect and quantify these parasites in food products, methods applied did not undergo through extensive validation process, a feature highly desirable or mandatory in the case of testing laboratories accredited for the ISO EN 17025:2005...
June 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28645883/the-first-nationwide-surveillance-of-antibacterial-susceptibility-patterns-of-pathogens-isolated-from-skin-and-soft-tissue-infections-in-dermatology-departments-in-japan
#5
Shinichi Watanabe, Takamitsu Ohnishi, Akira Yuasa, Hiroshi Kiyota, Satoshi Iwata, Mitsuo Kaku, Akira Watanabe, Junko Sato, Hideaki Hanaki, Motomu Manabe, Tamio Suzuki, Fujio Otsuka, Michiko Aihara, Ken Iozumi, Takeshi Tamaki, Yuichi Funada, Mikio Shinozaki, Motoko Kobayashi, Masaru Okuda, Go Kikyo, Kumi Kikuchi, Yoshitane Okada, Masanori Takeshima, Osamu Kaneko, Natsuki Ogawa, Rie Ito, Ryuhei Okuyama, Shinji Shimada, Tadamichi Shimizu, Naohito Hatta, Maeda Manabu, Kiyohiro Tsutsui, Toshihiro Tanaka, Yoshiki Miyachi, Hideo Asada, Fukumi Furukawa, Ichiro Kurokawa, Keiji Iwatsuki, Michihiro Hide, Masahiko Muto, Osamu Yamamoto, Hiroyuki Niihara, Kenji Takagaki, Yasuo Kubota, Koji Sayama, Shigetoshi Sano, Masutaka Furue, Takuro Kanekura
To investigate the trends of antimicrobial resistance in pathogens isolated from skin and soft-tissue infections (SSTI) at dermatology departments in Japan, a Japanese surveillance committee conducted the first nationwide survey in 2013. Three main organisms were collected from SSTI at 30 dermatology departments in medical centers and 10 dermatology clinics. A total of 860 strains - 579 of Staphylococcus aureus, 240 of coagulase-negative Staphylococci, and 41 of Streptococcus pyogenes - were collected and shipped to a central laboratory for antimicrobial susceptibility testing...
June 20, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28645768/robot-assisted-training-for-people-with-spinal-cord-injury-a-meta-analysis
#6
REVIEW
Eddy Yy Cheung, Thomas Kw Ng, Kevin Kk Yu, Rachel Lc Kwan, Gladys Ly Cheing
OBJECTIVE: To investigate the effects of robotic-assisted training on the recovery of people with spinal cord injury (SCI). DATA SOURCE: Randomized controlled trials (RCTs) or quasi-RCTs involving people with SCI that compared robotic-assisted upper limbs or lower limbs training to a control of other treatment approach or no treatment. We included studies involving people with complete or incomplete spinal cord injuries. STUDY SELECTION: We searched in Medline, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Cochrane Central Register of Controlled Trials (Cochrane Library) and Excerpta Medica dataBASE (EMBASE) to August, 2016...
June 20, 2017: Archives of Physical Medicine and Rehabilitation
https://www.readbyqxmd.com/read/28645479/impact-of-the-nap-1-strain-on-disease-severity-mortality-and-recurrence-of-healthcare-associated-clostridium-difficile-infection
#7
Karri A Bauer, Jessica E W Johnston, Eric Wenzler, Debra A Goff, Charles H Cook, Joan-Miquel Balada-Llasat, Preeti Pancholi, Julie E Mangino
OBJECTIVES: Studies are conflicting regarding the association of the North American pulsed-field gel electrophoresis type 1 (NAP1) strain in Clostridium difficile infection (CDI) and outcomes. We evaluated the association of NAP1 with healthcare-associated CDI disease severity, mortality, and recurrence at our academic medical center. METHODS: Healthcare-associated CDI cases were identified from November 1, 2011 through January 31, 2013. Multivariable regression models were used to evaluate the associations of NAP1 with severe disease (based on the Hines VA severity score index), mortality, and recurrence...
June 20, 2017: Anaerobe
https://www.readbyqxmd.com/read/28645366/optimization-of-native-and-formaldehyde-ipond-techniques-for-use-in-suspension-cells
#8
Nathaniel E Wiest, Alan E Tomkinson
The isolation of proteins on nascent DNA (iPOND) technique developed by the Cortez laboratory allows a previously unparalleled ability to examine proteins associated with replicating and newly synthesized DNA in mammalian cells. Both the original, formaldehyde-based iPOND technique and a more recent derivative, accelerated native iPOND (aniPOND), have mostly been performed in adherent cell lines. Here, we describe modifications to both protocols for use with suspension cell lines. These include cell culture, pulse, and chase conditions that optimize sample recovery in both protocols using suspension cells and several key improvements to the published aniPOND technique that reduce sample loss, increase signal to noise, and maximize sample recovery...
2017: Methods in Enzymology
https://www.readbyqxmd.com/read/28645285/completeness-and-timeliness-of-notifiable-disease-reporting-a-comparison-of-laboratory-and-provider-reports-submitted-to-a-large-county-health-department
#9
Brian E Dixon, Zuoyi Zhang, Patrick T S Lai, Uzay Kirbiyik, Jennifer Williams, Rebecca Hills, Debra Revere, P Joseph Gibson, Shaun J Grannis
BACKGROUND: Most public health agencies expect reporting of diseases to be initiated by hospital, laboratory or clinic staff even though so-called passive approaches are known to be burdensome for reporters and produce incomplete as well as delayed reports, which can hinder assessment of disease and delay recognition of outbreaks. In this study, we analyze patterns of reporting as well as data completeness and timeliness for traditional, passive reporting of notifiable disease by two distinct sources of information: hospital and clinic staff versus clinical laboratory staff...
June 23, 2017: BMC Medical Informatics and Decision Making
https://www.readbyqxmd.com/read/28645207/development-and-validation-of-an-electronic-medical-record-emr-based-computed-phenotype-of-hiv-1-infection
#10
Devon W Paul, Nigel B Neely, Meredith Clement, Isaretta Riley, Mashael Al-Hegelan, Matthew Phelan, Monica Kraft, David M Murdoch, Joseph Lucas, John Bartlett, Mehri McKellar, Loretta G Que
Background: Electronic medical record (EMR) computed algorithms allow investigators to screen thousands of patient records to identify specific disease cases. No computed algorithms have been developed to detect all cases of human immunodeficiency virus (HIV) infection using administrative, laboratory, and clinical documentation data outside of the Veterans Health Administration. We developed novel EMR-based algorithms for HIV detection and validated them in a cohort of subjects in the Duke University Health System (DUHS)...
June 22, 2017: Journal of the American Medical Informatics Association: JAMIA
https://www.readbyqxmd.com/read/28645195/mutational-biases-influence-parallel-adaptation
#11
Arlin Stoltzfus, David M McCandlish
While mutational biases strongly influence neutral molecular evolution, the role of mutational biases in shaping the course of adaptation is less clear. Here we consider the frequency of transitions relative to transversions among adaptive substitutions. Because mutation rates for transitions are higher than those for transversions, if mutational biases influence the dynamics of adaptation, then transitions should be over-represented among documented adaptive substitutions. To test this hypothesis, we assembled 2 sets of data on putatively adaptive amino acid replacements that have occurred in parallel during evolution, either in nature or in the laboratory...
June 22, 2017: Molecular Biology and Evolution
https://www.readbyqxmd.com/read/28645094/quality-assessment-of-shoulder-plyometric-exercises-examining-the-relationship-to-scapular-muscle-activity
#12
Maya Calé-Benzoor, Annelies Maenhout, Michal Arnon, Gershon Tenenbaum, Mia Werrin, Ann Cools
OBJECTIVES: The purpose of the study was to evaluate performance quality of shoulder plyometric exercises, and examine the relationship to scapular muscle activation during an intense exercise bout. DESIGN: Observational study. SETTING: University laboratory. PARTICIPANTS: 32 healthy university students (male/female: 14/18) volunteers. MAIN OUTCOME MEASURES: Subjects performed 10 plyometric exercises...
May 12, 2017: Physical Therapy in Sport
https://www.readbyqxmd.com/read/28644889/evaluation-of-different-serological-assays-for-early-diagnosis-of-leptospirosis-in-martinique-french-west-indies
#13
Christophe Courdurie, Yohann Le Govic, Pascale Bourhy, Dorothee Alexer, Karine Pailla, Rafaelle Theodose, Raymond Cesaire, Jacques Rosine, Patrick Hochedez, Claude Olive
BACKGROUND: Leptospirosis is a potentially life-threatening but curable zoonosis whose prognosis depends on accurate and timely diagnosis. Because of its non-specific clinical presentation, laboratory testing is essential to confirm the diagnosis. Here, we aimed to assess the performance of two enzyme-linked immunosorbent assays (ELISAs) (ELISA Serion and ELISA-Hb Pasteur) and one immunodot (GenBio) using quantitative PCR (qPCR) as gold standard, instead of the traditional microscopic agglutination test, for the diagnosis of acute leptospirosis in an endemic area...
June 23, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28644345/yield-of-endoscopy-in-pediatric-gastroparesis
#14
Tara A Altepeter, Stephen Shaffer
OBJECTIVES: The aim of the study was to quantify the diagnostic yield of upper endoscopy in children with gastroparesis and to develop a clinical model for gastroparesis using common symptoms and screening blood tests. METHODS: We retrospectively reviewed charts of 196 patients of age 4 to 18 years evaluated for gastroparesis between 2009 and 2013. All patients completed a standard solid-phase gastric emptying scan and upper endoscopy within a 12-month period. We analyzed gross and histologic endoscopy findings...
July 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28644301/palliative-oxygen-for-chronic-breathlessness-what-new-evidence
#15
Zainab Ahmadi, David C Currow, Magnus Ekström
PURPOSE OF REVIEW: Supplemental oxygen improves survival in patients with chronic obstructive pulmonary disease (COPD) and severe hypoxaemia, but the effect of oxygen therapy in mild or moderate hypoxaemia to reduce symptomatic chronic breathlessness remains unclear. This review provides an overview of recent evidence about the role of oxygen therapy for the relief of chronic breathlessness in advanced illness. RECENT FINDINGS: In COPD, a recent Cochrane review strengthens earlier findings regarding the positive effect of supplemental oxygen compared with air during exercise test in the training setting...
July 20, 2017: Current Opinion in Supportive and Palliative Care
https://www.readbyqxmd.com/read/28644253/spectrum-of-autonomic-nervous-system-impairment-in-sj%C3%A3-gren-syndrome
#16
Brent P Goodman, Amy Crepeau, Priya S Dhawan, Julie A Khoury, Lucinda A Harris
OBJECTIVE: To describe the spectrum of autonomic dysfunction in a uniformly evaluated cohort of patients with Sjögren syndrome. METHODS: A series of 13 patients underwent a comprehensive evaluation for suspected autonomic impairment, including a neurological examination, autonomic testing, and laboratory studies. A diagnosis of Sjögren syndrome was established as the cause of autonomic dysfunction in all. Clinical features, findings on autonomic testing, and laboratory results are described...
July 2017: Neurologist
https://www.readbyqxmd.com/read/28644049/the-standard-treatment-protocol-for-paracetamol-poisoning-may-be-inadequate-following-overdose-with-modified-release-formulation-a-pharmacokinetic-and-clinical-analysis-of-53-cases
#17
Heléne Salmonson, Gunilla Sjöberg, Jacob Brogren
OBJECTIVE: The use of the standard procedure for managing overdoses with immediate release (IR) paracetamol is questionable when applied to overdoses with modified release (MR) formulations. This study describes the pharmacokinetics of paracetamol and the clinical outcomes following overdoses with a MR formulation. METHODS: Medical records including laboratory analyses concerning overdoses of MR paracetamol from 2009 to 2015 were collected retrospectively. Inclusion criteria were ingestion of a toxic dose, known time of intake and documented measurements of serum paracetamol and liver function tests...
June 23, 2017: Clinical Toxicology
https://www.readbyqxmd.com/read/28643919/mechanobehaviour-in-dolichofacial-and-brachyfacial-adolescents
#18
J C Nickel, A L Weber, P Covington Riddle, Y Liu, H Liu, L R Iwasaki
OBJECTIVES: To test whether mechanobehaviour (temporomandibular joint (TMJ) loads, jaw muscle use) was different between facial types and correlated with ramus height (Condylion-Gonion, mm). SETTING AND SAMPLE POPULATION: University of Missouri-Kansas City (UMKC) Orthodontic Clinic. Ten dolichofacial and ten brachyfacial adolescents (Sella-Nasion-Gonion-Gnathion (SN-GoGn) angles ≥37° and ≤27°, respectively) consented to participate. MATERIALS AND METHODS: Numerical models calculated TMJ loads for a range of static biting based on subjects' three-dimensional anatomy...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28643629/faecal-immunochemical-tests-to-triage-patients-with-lower-abdominal-symptoms-for-suspected-colorectal-cancer-referrals-in-primary-care-a-systematic-review-and-cost-effectiveness-analysis
#19
Marie Westwood, Isaac Corro Ramos, Shona Lang, Marianne Luyendijk, Remziye Zaim, Lisa Stirk, Maiwenn Al, Nigel Armstrong, Jos Kleijnen
BACKGROUND: Colorectal cancer (CRC) is the third most common cancer in the UK. Presenting symptoms that can be associated with CRC usually have another explanation. Faecal immunochemical tests (FITs) detect blood that is not visible to the naked eye and may help to select patients who are likely to benefit from further investigation. OBJECTIVES: To assess the effectiveness of FITs [OC-Sensor (Eiken Chemical Co./MAST Diagnostics, Tokyo, Japan), HM-JACKarc (Kyowa Medex/Alpha Laboratories Ltd, Tokyo, Japan), FOB Gold (Sentinel/Sysmex, Sentinel Diagnostics, Milan, Italy), RIDASCREEN Hb or RIDASCREEN Hb/Hp complex (R-Biopharm, Darmstadt, Germany)] for primary care triage of people with low-risk symptoms...
May 2017: Health Technology Assessment: HTA
https://www.readbyqxmd.com/read/28643557/pooled-sample-testing-for-bonamia-ostreae-a-tale-of-two-sybr-green-real-time-pcr-assays
#20
Henry S Lane, J Brian Jones, Wendy L McDonald
Pooled testing of samples is a common laboratory practice to increase efficiency and reduce expenses. We investigated the efficacy of 2 published SYBR Green real-time PCR assays when used to detect the haplosporidian parasite Bonamia ostreae in pooled samples of infected oyster tissue. Each PCR targets a different gene within the B. ostreae genome: the actin 1 gene or the 18S rRNA gene. Tissue homogenates (150 mg) of the New Zealand flat oyster Ostrea chilensis were spiked with ~1.5 × 10(3) purified B. ostreae cells to create experimental pools of 3, 5, and 10...
June 1, 2017: Journal of Veterinary Diagnostic Investigation
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