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Mikio Sugiki, Hiroyuki Miyakawa, Shoichi Tahara, Sumiyo Yamamoto, Narue Sakamaki, Yuki Sadamasu, Yoko Uematsu, Kimio Monma
A quantitative analysis by HPLC of α-glucosyltransferase-treated stevia in foods was considered. This analysis is the way which hydrolyzed α-glucosyltransferase-treated stevia in the stevioside (SS) and the rebaudioside A (RS) using a glucoamylase. Recovery (%) of α-glucosyltransferase-treated stevia, spiked at 200 mg/kg in various foods, were more than 80% and the relative standard deviations were less than 5.0% as SS and RS for the rate of collection. A qualitative analysis by LC-MS/MS was performed 36 products of commercial foods containing stevia...
2018: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
Liangliang Chen, Ping Sun, Fangfang Zhou, Yan Li, Kequan Chen, Honghua Jia, Ming Yan, Dachun Gong, Pingkai Ouyang
Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4-0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside. In the present study, pathways for rebaudioside D synthesis and UDP-glucose recycling were developed by coupling recombinant UDP-glucosyltransferase UGTSL2 from Solanum lycopersicum and sucrose synthase StSUS1 from Solanum tuberosum...
September 1, 2018: Food Chemistry
Juliana Peres, Erick Esmerino, Alessandra Lins da Silva, Ilana Racowski, Helena Bolini
The objective of this study was to evaluate the sensory profile and the influence of the information on the acceptance of the symbiotic chocolate ice cream made with sucrose and different sweeteners (aspartame, sucralose, neotame, Stevia with 60%, 85%, 95%, and 97% of rebaudioside A) through analysis of variance (ANOVA), Tukey's test, and partial least of square (PLS) regression. Quantitative descriptive analysis (QDA) was carried out by 18 assessors, who evaluated the samples in relation to the raised descriptors...
April 16, 2018: Journal of Food Science
Rebekka Thøgersen, Bjørn Petrat-Melin, Galia Zamaratskaia, Kai Grevsen, Jette Feveile Young, Martin Krøyer Rasmussen
The physiological effects of the Stevia-derived compounds, rebaudioside A, stevioside and steviol have been the focus of several studies due to their use as sweeteners in food. Despite that, little is known about their potential food-drug interactions. In the present study, IPEC-J2 cells and primary hepatocytes were used to investigate the effect of rebaudioside A, stevioside and steviol on cytochrome p450 (CYP) mRNA expression. Moreover, hepatic microsomes were used to investigate direct interactions between the compounds and specific CYP activity...
August 30, 2018: Food Chemistry
Nongluck Tangpaisarnkul, Patoomratana Tuchinda, Prapin Wilairat, Atitaya Siripinyanond, Juwadee Shiowattana, Saksit Nobsathian
Pure stevioside was extracted from leaves of Stevia rebaudiana (Bertoni) to be used as a reference material in the instrument calibration or method validation process. The mass fraction was determined by comparison between the mass balance method and quantitative nuclear magnetic resonance (qNMR) spectroscopy. The impurities in the sample were analyzed by Karl Fischer titration for moisture content and thermogravimetric analysis for inorganic residue. Homogeneity, together with short term and long term stability, were also studied and the uncertainty was reported...
July 30, 2018: Food Chemistry
Ernestina Hernández García, Norma Osnaya Brizuela, Armando Valenzuela Peraza, David Calderón Guzmán, Maribel Ortiz Herrera, Hugo Juárez Olguín, Gerardo Barragán Mejía, Daniel Santamaría Del Ángel, Alberto Rojas Ochoa
OBJECTIVE: The aim of this study was to evaluate the effect of splenda and stevia on dopamine and 5-HIAA levels, and some biomarkers of oxidative stress in the presence of cytarabine. METHODS: Forty-eight young male Wistar rats each with a weight of 80 g (four weeks of age), distributed in six groups of eight animals each, were treated as follows: group 1, control (NaCl 0.9% vehicle); group 2, cytarabine (0.6 g/kg); group 3, stevia (0.6 g/kg); group 4, cytarabine + stevia; group 5, splenda; and group 6, cytarabine + splenda...
February 13, 2018: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Danijela Bursać Kovačević, Francisco J Barba, Daniel Granato, Charis M Galanakis, Zoran Herceg, Verica Dragović-Uzelac, Predrag Putnik
Stevia rebaudiana Bertoni leaves are a natural source of diterpenic glycosides, and various bioactive compounds. The objectives were to characterize antioxidants and steviol glycosides in the extracts obtained from Stevia after "green" pressurized hot water extraction (PHWE). PHWE extracts were obtained at different temperatures (100, 130, 160 °C); static extraction times (5 and 10 min), and cycle numbers (1, 2, 3) using a constant pressure of 10.34 MPa. Temperature was the most important parameter for extraction, where the highest recoveries of all bioactive compounds (except for carotenoids) were at 160 °C...
July 15, 2018: Food Chemistry
Shokoofeh Hajihashemi, Shakiba Rajabpoor, Ivica Djalovic
The study on Stevia callus has the potential to advance the knowledge of antioxidant mechanisms involved in unorganized cells response to drought stress. The effects of polyethylene glycol (PEG; 0 and 4% w/v) in combination with paclobutrazol (PBZ; 0 and 2 mg l-1 ) and gibberellin (GA; 0 and 2 mg l-1 ) were studied on Stevia rebaudiana callus. PEG treatment led to an oxidative stress, as indicated by increased H2 O2 content whose accumulation was prevented with PBZ and GA treatments. All treatments of PEG, PBZ and GA increased the total antioxidant capacity, with the highest antioxidant power in PBZ and GA treatments without PEG...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Samuel Durán Agüero, Lissé Angarita Dávila, Ma Cristina Escobar Contreras, Diana Rojas Gómez, Jorge de Assis Costa
Noncaloric sweeteners (NCS) are food additives used to provide sweetness without adding calories. Their consumption has become more widespread around the world in all age groups, including children. The aim of this study is to show the state of the art about the intake of noncaloric sweeteners in children, as well as their benefits and consumption risk. Scientific searchers were used (PUBMED, Scopus, and Scielo) to analyze articles that included keywords (noncaloric sweeteners/saccharin/cyclamate/acesulfame potassium/aspartame/sucralose/stevia/children) in English, Spanish, and Portuguese...
2018: BioMed Research International
Sandra Nicklasson, Desirée Sjöström, Mats Amundin, Daniel Roth, Laura Teresa Hernandez Salazar, Matthias Laska
Primates have been found to differ widely in their taste perception and studies suggest that a co-evolution between plant species bearing a certain taste substance and primate species feeding on these plants may contribute to such between-species differences. Considering that only platyrrhine primates, but not catarrhine or prosimian primates, share an evolutionary history with the neotropical plant Stevia rebaudiana , we assessed whether members of these three primate taxa differ in their ability to perceive and/or in their sensitivity to its two quantitatively predominant sweet-tasting substances...
February 2018: Current Zoology
Alexis Lacout, Véronique Dacher, Mostafa El Hajjam, Pierre Yves Marcy, Christian Perronne
Lyme disease is an affection caused by a spirochete infection called Borrelia Burgdorferi which may harbor a varied and misleading clinical symptomatology. The serology tests commonly used for diagnosis show a wide sensitivity varying from 34% to 70,5%, leaving many infected patients with false negative tests. Alternative techniques such as polymerase chain reaction (PCR) could be helpful but not conclusive enough. Using biofilm busters, such as stevia and serratiopeptidase, could lead to bacterial blood release, thus increasing the spirochete load, making PCR test more sensitive, thus improving the patient's diagnosis and management...
March 2018: Medical Hypotheses
Mehrangiz Akbari, Mohammad Eghbal Ghobadi, Mokhtar Ghobadi, Said Jalali-Honarmand, Mohsen Saeidi
Stevia (Stevia rebaudiana B.) has auxiliary buds that often remain dormant for a long time and sometimes remain dormant until the plants change at the reproductive stage. This study was designed out to investigate whether decapitation and exogenous application of plant growth regulators enhance the productivity of stevia through breaking the apical dominance and increasing physiological characteristics. Experiment was carried out as a factorial in randomized complete block design with three replications. Factors were consisted two agricultural practices (Decapitation and No-decapitation) and eight foliar spray including without spray as control, water spray, GA3 (300, 600 and 900 µm) and CK (100, 200 and 400 µm)...
February 10, 2018: Cellular and Molecular Biology
Tahereh Keshvari, Abdollah Najaphy, Danial Kahrizi, Alireza Zebarjadi
Stevia rebaudiana (Bert.) from Asteraceae family is a useful medicinal plant that prevents and cures diabetes, blood pressure, weight gain and tooth decay. Due to self-incompatibility in stevia, somatic embryo investigation for artificial seed production is valuable in this plant. In order to evaluate the callus induction characteristics in stevia, a factorial experiment was laid out based on a completely randomized design with three replications. The factors included ten hormone combinations and control, two kinds of media (MS and B5) and two types of explants (leaf and internode)...
February 10, 2018: Cellular and Molecular Biology
Mehmet Atas, Nuraniye Eruygur, Esra Ucar, Yasar Ozyigit, Kenan Turgut
In this study, the efficiency of the different nitrogen doses (0, 5, 10, 15 and 20 kg ha-1) on biological activity levels (antioxidant and antimicrobial activity) of Stevia rebaudiana Bert. was investigated. In addition, methanol extracts were obtained by maceration method from different doses of fertilizer applied stevia. The components in methanol extracts of plants were determined by gas chromatography-mass spectrometry (GC-MS) method. Antimicrobial activities of stevia extracts were investigated by microdilution method...
February 10, 2018: Cellular and Molecular Biology
Alireza Mirzaei, Gholamreza Kavoosi, Sara Dezhsetan, Mahdi Behnamian
Stevia rebaudiana Bertoni is a kind of perennial medicinal plant with sweetening properties which belongs to Asteraceae family. Its leaves with fundamental glycoside compounds consist of both a sugar part and a non-sugar sector. One of the glycoside compounds is Rebaudioside- A which has a greater importance in business. This experiment was conducted to evaluate the effects of Ag2O, CrO3, PbO, Fe2O3, BaO and TiO2 on the expression pattern of these genes in the Stevia rebaudiana. Rebaudioside- A biosynthesis was repeated 3 times with concentrations of 50, 100 and 200µM...
February 10, 2018: Cellular and Molecular Biology
Somaye Hashempoor, Matin Ghaheri, Danial Kahrizi, Nastaran Kazemi, Saare Muhammadi, Seyed Mehdi Safavi, Tayebeh Ghorbani, Elham Rahmanian, Malihe Heshmatpanaah
Stevia rebaudiana Bertoni is one of the most important herbal sweetener plants from Asteracea family that have a lot of Steviol glycosides. Among different methods, tissue culture is the best way with high efficiency that is useful for studying stress tolerance mechanisms to obtain drought tolerance of stevia. For this purpose, different concentrations of mannitol (0, 10, 20, 30, 40, 50 mg/l) were used as various treatments in the culture medium of stevia. According to the results, the highest level of UGT85C2 gene expression (1...
February 10, 2018: Cellular and Molecular Biology
Danial Kahrizi, Matin Ghaheri, Zahra Yari, Khirollah Yari, Sohbat Bahraminejad
Stevia rebaudiana Bertoni is one of two species that contains steviol glycosides. Among steviol glycosides that extracted from leaves, stevioside and rebaudioside A are the two major and the sweetest glycosides that are about 200-300 times sweeter than sucrose with zero calories. The best method for stevia propagation is tissue culture. So, for investigation of nutrients in medium, we studied the effect of different concentrations of MS media (MS, 0.5 MS, 0.25 MS, 0 MS) on morphological traits, UGT74G1 and UGT76G1 genes expression and accumulation of steviol glycosides in stevia leaves...
February 10, 2018: Cellular and Molecular Biology
Matin Ghaheri, Elaheh Adibrad, Seyed Mehdi Safavi, Danial Kahrizi, Ali Soroush, Saare Muhammadi, Tayebeh Ghorbani, Ali Sabzevari, Zahra Ansarypour, Elham Rahmanian
Stevia rebaudiana Bertoni is One of the most important biologically sourced and low-calorie sweeteners that known as "Sweet Weed". It contains steviol glycosides that they are about 200-300 times sweeter than sucrose. Tissue culture is the best method with high efficiency that can overcome to problems of traditional methods, and it is the most useful tools for studying stress tolerance mechanisms under in vitro conditions to obtain drought tolerance. In the present research, we investigated the impact of life cycle, leaves location and the harvesting time on expression of UGT74G1 and UGT76G1 as well as steviol glycosides accumulation...
February 10, 2018: Cellular and Molecular Biology
Fariba Akbari, Ali Arminian, Danial Kahrizi, Arash Fazeli, Matin Ghaheri
Stevia rebaudiana (Bertoni) is a non-caloric sweetener belonging to Asteraceae family. Stevia compounds such as steviol glycosides (SGs) are 200 times sweeter than sugar. Stevioside and rebaudioside A are the two major steviol glycosides. Nitrogen is an essential element for plant growth and development. In this study the effects of nitrogen influenced by different concentrations of NH4NO3 (0, 825 and 1650 mg/l) and KNO3 (0, 950 and 1900 mg/l) is examined in MS medium. To analysis the UGT74G1 and UGT76G1 genes expression, involved in the synthesis of SGs, RT-qPCR technique was performed...
February 10, 2018: Cellular and Molecular Biology
Matin Ghaheri, Shahram Miraghaee, Atefeh Babaei, Bahareh Mohammadi, Danial Kahrizi, Zahra Minoosh Saivosh Haghighi, Gholamreza Bahrami
Stevia rebaudiana Bertoni has been used locally as a non-calorie sweetener in medicine and diabetic diet which claimed to have aphrodisiac properties, although no scientific data of this function have been reported. The aim of this study was to investigate the effect of S. rebaudiana extract on sexual dysfunction, testosterone levels and number of Leydig cells in Streptozotocin (STZ)-induced diabetic male rats. A total of 28 diabetic male rats were randomly divided into 4 groups: diabetic group without any extract and 3 extract groups (5, 50 and 100 mg/kg)...
February 10, 2018: Cellular and Molecular Biology
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