keyword
MENU ▼
Read by QxMD icon Read
search

Glycosyltransferase

keyword
https://www.readbyqxmd.com/read/28229927/alteration-of-n-glycan-expression-profile-and-glycan-pattern-of-glycoproteins-in-human-hepatoma-cells-after-hcv-infection
#1
Tian Xiang, Ganglong Yang, Xiaoyu Liu, Yidan Zhou, Zhongxiao Fu, Fangfang Lu, Jianguo Gu, Naoyuki Taniguchi, Zengqi Tan, Xi Chen, Yan Xie, Feng Guan, Xiao-Lian Zhang
BACKGROUND: Hepatitis C virus (HCV) infection causes chronic liver diseases, liver fibrosis and even hepatocellular carcinoma (HCC). However little is known about any information of N-glycan pattern in human liver cell after HCV infection. METHODS: The altered profile of N-glycans in HCV-infected Huh7.5.1 cell were analyzed by using mass spectrometry. Then, lectin microarray, lectin pull-down assay, reverse transcription-quantitative real time PCR (RT-qPCR) and western-blotting were used to identify the altered N-glycosylated proteins and glycosyltransferases...
February 13, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28224916/transcriptional-markers-enable-identification-of-rye-grass-lolium-sp-plants-with-non-target-site-based-resistance-to-herbicides-inhibiting-acetolactate-synthase
#2
Arnaud Duhoux, Sébastien Carrère, Alexis Duhoux, Christophe Délye
Molecular detection of herbicide non-target-site-based resistance (NTSR) classically requires extensively validated NTSR genes. We assessed the feasibility of predicting NTSR phenotypes using expression data of NTSR transcriptional markers, i.e., transcripts which expression levels are statistically correlated to NTSR. Markers were sought by comparative RNA-Seq analysis of untreated NTSR or sensitive plants from four rye-grass populations followed by expression quantification in 299 individual plants with characterised sensitivity to two acetolactate-synthase-inhibiting herbicides...
April 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28222092/the-genetic-basis-of-resistance-and-matching-allele-interactions-of-a-host-parasite-system-the-daphnia-magna-pasteuria-ramosa-model
#3
Gilberto Bento, Jarkko Routtu, Peter D Fields, Yann Bourgeois, Louis Du Pasquier, Dieter Ebert
Negative frequency-dependent selection (NFDS) is an evolutionary mechanism suggested to govern host-parasite coevolution and the maintenance of genetic diversity at host resistance loci, such as the vertebrate MHC and R-genes in plants. Matching-allele interactions of hosts and parasites that prevent the emergence of host and parasite genotypes that are universally resistant and infective are a genetic mechanism predicted to underpin NFDS. The underlying genetics of matching-allele interactions are unknown even in host-parasite systems with empirical support for coevolution by NFDS, as is the case for the planktonic crustacean Daphnia magna and the bacterial pathogen Pasteuria ramosa...
February 21, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28220704/functional-characterization-of-a-new-tea-camellia-sinensis-flavonoid-glycosyltransferase
#4
Xianqian Zhao, Peiqiang Wang, Mingzhuo Li, Yeru Wang, Xiaolan Jiang, Lilan Cui, Yumei Qian, Juhua Zhuang, Liping Gao, Tao Xia
Tea (Camellia sinensis) is an important commercial crop, in which the high content of flavonoids provides the health benefits. A flavonoid glycosyltransferase (CsUGT73A20), belonged to Cluster IIIa was isolated from tea plant. The recombinant CsUGT73A20 in E. coli exhibited a broad substrate tolerance towards multiple flavonoids. Among them, kaempferol was the optimal substrate compared to quercetin, myricetin, naringenin, apigenin and kaempferide. However, no product was detected when UDP-galactose was used as the sugar donor...
February 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28219734/position-228-in-paenibacillus-macerans-cyclodextrin-glycosyltransferase-is-critical-for-2-o-d-glucopyranosyl-l-ascorbic-acid-synthesis
#5
Sheng Chen, Yanjun Xiong, Lingqia Su, Lei Wang, Jing Wu
The markedly stable l-ascorbic acid (L-AA) derivative 2-O-d-glucopyranosyl-l-ascorbic acid (AA-2G) has been widely used in the fields of food, medicine, cosmetics, and husbandry. Cyclodextrin glycosyltransferase (CGTase) is considered suitable for the large-scale production of AA-2G. In this work, Paenibacillus macerans CGTase was used to produce AA-2G and the production was 13.5g/l. An amino-acid sequence alignment of α-, β-, and α⁄β-CGTase indicated that the Phe at position 228 of P. macerans CGTase was different from the amino acids at this position in other CGTases (Met, Val, or Ile)...
February 17, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28219382/high-level-extracellular-protein-production-in-bacillus-subtilis-using-an-optimized-dual-promoter-expression-system
#6
Kang Zhang, Lingqia Su, Xuguo Duan, Lina Liu, Jing Wu
BACKGROUND: We recently constructed a Bacillus subtilis strain (CCTCC M 2016536) from which we had deleted the srfC, spoIIAC, nprE, aprE and amyE genes. This strain is capable of robust recombinant protein production and amenable to high-cell-density fermentation. Because the promoter is among the factors that influence the production of target proteins, optimization of the initial promoter, PamyQ from Bacillus amyloliquefaciens, should improve protein expression using this strain. This study was undertaken to develop a new, high-level expression system in B...
February 20, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28215607/impact-of-blue-red-and-far-red-light-treatments-on-gene-expression-and-steviol-glycoside-accumulation-in-stevia-rebaudiana
#7
Yuki Yoneda, Hiroshi Nakashima, Juro Miyasaka, Katsuaki Ohdoi, Hiroshi Shimizu
Stevia rebaudiana (Bertoni) Bertoni is a plant that biosynthesizes a group of natural sweeteners that are up to approximately 400 times sweeter than sucrose. The sweetening components of S. rebaudiana are steviol glycosides (SGs) that partially share their biosynthesis pathway with gibberellins (GAs). However, the molecular mechanisms through which SGs levels can be improved have not been studied. Therefore, transcription levels of several SG biosynthesis-related genes were analyzed under several light treatments involved in GA biosynthesis...
February 15, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28213905/a-ten-gene-containing-genomic-island-determines-flagellin-glycosylation-implication-for-its-regulatory-role-in-motility-and-virulence-of-xanthomonas-oryzae-pv-oryzae
#8
Chao Yu, Huamin Chen, Fang Tian, Fenghuan Yang, Xiaochen Yuan, Ching-Hong Yang, Chenyang He
Flagellin glycosylation plays crucial roles in flagellar assembly, motility and virulence in several pathogenic bacteria. However, little is known about the genetic determinants and biological functions of flagellin glycosylation in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Here, the structure, regulation and functions of a ten-gene cluster gigX (glycosylation island genes of Xoo), which was embedded in a flagellar regulon were characterized. gigX1 through gigX10, encoded putative enzymes or proteins involved in glycan biosynthesis and transfer, including a nucleotide sugar transaminase, an acyl-carrier protein (ACP), a 3-oxoacyl-ACP synthase, a 3-oxoacyl-ACP reductase, a dehydrogenase, an acetyltransferase, a ring hydroxylating dioxygenase, a hypothetical protein, a methyltransferanse, and a glycosyltransferase, respectively...
February 18, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28207970/molecular-characterization-of-the-c-glucosylation-for-puerarin-biosynthesis-in-pueraria-lobata
#9
Xin Wang, Changfu Li, Chen Zhou, Jia Li, Yansheng Zhang
C-glycosyltransferases (CGTs) are important enzymes that are responsible for the synthesis of the C-glycosides of flavonoids and isoflavonoids. Flavonoid CGTs have been molecularly characterized from several plant species; however, to date, no gene encoding an isoflavonoid CGT has been reported from any plant species. A significant example of an isoflavonoid C-glycoside is puerarin, a compound that contributes to the major medicinal effects of Pueraria lobata. Knowledge regarding the C-glucosylation that occurs during puerarin biosynthesis remains scant...
February 16, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28194155/functional-characterization-of-cyp716-family-p450-enzymes-in-triterpenoid-biosynthesis-in-tomato
#10
Shuhei Yasumoto, Hikaru Seki, Yuko Shimizu, Ery O Fukushima, Toshiya Muranaka
Triterpenoids are a group of structurally diverse specialized metabolites that frequently show useful bioactivities. These chemicals are biosynthesized from the common precursor 2,3-oxidosqualene in plants. The carbon skeletons produced by oxidosqualene cyclase (OSC) are usually modified by cytochrome P450 monooxygenases (P450s) and UDP-dependent glycosyltransferases. These biosynthetic enzymes contribute to the structural diversification of plant triterpenoids. Until now, many P450 enzymes have been characterized as triterpenoid oxidases...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28191923/one-pot-combinatorial-biosynthesis-of-glycosylated-anthracyclines-by-co-cultivation-of-streptomyces-strains-producing-aglycones-and-nucleotide-deoxysugars
#11
Eunji Kim, Myoung Chong Song, Myoun Su Kim, Ji Yoon Beom, Jin A Jung, Hang Soo Cho, Yeo Joon Yoon
Anthracyclines such as doxorubicin are effective anticancer drugs composed of a tetracyclic polyketide aglycone and one or more deoxysugar moieties, which play a critical role in their biological activity. A facile one-pot combinatorial biosynthetic system was developed for the generation of a range of glycosylated derivatives of anthracyclines. Co-cultivation of Streptomyces venezuelae mutants producing two anthracycline aglycones with eight different nucleotide deoxysugar-producing S. venezuelae mutants that co-express a substrate-flexible glycosyltransferase led to the generation of sixteen aklavinone or ε-rhodomycinone glycosides containing diverse deoxysugar moieties, seven of which are new...
February 13, 2017: ACS Combinatorial Science
https://www.readbyqxmd.com/read/28179475/asparagine-linked-glycans-of-cryptosporidium-parvum-contain-a-single-long-arm-are-barely-processed-in-the-er-or-golgi-and-show-a-strong-bias-for-sites-with-threonine
#12
John R Haserick, Deborah R Leon, John Samuelson, Catherine E Costello
Cryptosporidium parvum causes severe diarrhea in infants in developing countries and in immunosuppressed persons, including those with AIDS. We are interested in the Asn-linked glycans (N-glycans) of C. parvum, because 1) the N-glycan precursor is predicted to contain five mannose and two glucose residues on a single long arm versus nine mannose and three glucose residues on the three-armed structure common in host N-glycans, 2) C. parvum is a rare eukaryote that lacks the machinery for N-glycan-dependent quality control of protein folding in the lumen of the Endoplasmic Reticulum (ER), and 3) ER and Golgi mannosidases, as well as glycosyltransferases that build complex N-glycans, are absent from the predicted proteome...
February 8, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28179078/serum-resistance-and-phase-variation-of-a-nasopharyngeal-non-typeable-haemophilus-influenzae-isolate
#13
Sabine Lichtenegger, Isabelle Bina, Sanel Durakovic, Philippe Glaser, Sarah Tutz, Stefan Schild, Joachim Reidl
Haemophilus influenzae harbours a complex array of factors to resist human complement attack. As non-typeable H. influenzae (NTHi) strains do not possess a capsule, their serum resistance mainly depends on other mechanisms including LOS decoration. In this report, we describe the identification of a highly serum resistant, nasopharyngeal isolate (NTHi23) by screening a collection of 77 clinical isolates. For NTHi23, we defined the MLST sequence type 1133, which matches the profile of a previously published invasive NTHi isolate...
January 30, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28178702/dysregulation-of-the-expression-of-asparagine-linked-glycosylation-13-short-isoform-2-affects-nephrin-function-by-altering-its-n-linked-glycosylation
#14
Teresa Esposito, Giovanni De Stefano, Mafalda Giovanna Reccia, Ilaria Di Lorenzo, Filomena Napolitano, Francesco Scalabrì, Alessia Lombardi, Moin A Saleem, Lyn R Griffiths, Fernando Gianfrancesco
BACKGROUND: N-linked glycosylation, which is a post-translational modification process, plays an important role in protein folding, intracellular trafficking and membrane targeting, as well as in regulating the protein function. Recently, we identified a missense variant (p.T141L) in the short isoform 2 of the X-linked gene asparagine-linked glycosylation 13 (ALG13-is2), which segregated with focal segmental glomerulosclerosis and PCCD in a large Australian pedigree; however, any evidence of its pathogenicity was demonstrated...
February 9, 2017: Nephron
https://www.readbyqxmd.com/read/28177478/rapid-screening-of-sugar-nucleotide-donor-specificities-of-putative-glycosyltransferases
#15
M Osman Sheikh, Stephanie M Halmo, Sneha Patel, Dustin Middleton, Hideyuki Takeuchi, Christopher M Schafer, Christopher M West, Robert S Haltiwanger, Fikri Y Avci, Kelley W Moremen, Lance Wells
No abstract text is available yet for this article.
March 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/28167670/salmonella-typhi-lipopolysaccharide-o-antigen-modifications-impact-on-serum-resistance-and-antibody-recognition
#16
Erica Kintz, Christian Heiss, Ian Black, Nicholas Donohue, Naj Brown, Mark R Davies, Parastoo Azadi, Stephen Baker, Paul M Kaye, Marjan van der Woude
Salmonella Typhi is a human restricted Gram-negative bacterial pathogen, responsible for causing an estimated 27 million cases of typhoid fever annually leading to 217,000 deaths, and current vaccines do not offer full protection. The O-antigen side chain of the lipopolysaccharide is an immunodominant antigen, can define host-pathogen interactions, and is under consideration as a vaccine target for some Gram-negative species. The composition of the O-antigen can be modified by activity of glycosyltransferase (gtr) operons acquired by horizontal gene transfer...
February 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28166737/de-novo-transcriptome-assembly-analysis-of-weed-apera-spica-venti-from-seven-tissues-and-growth-stages
#17
Marielle Babineau, Khalid Mahmood, Solvejg K Mathiassen, Per Kudsk, Michael Kristensen
BACKGROUND: Loose silky bentgrass (Apera spica-venti) is an important weed in Europe with a recent increase in herbicide resistance cases. The lack of genetic information about this noxious weed limits its biological understanding such as growth, reproduction, genetic variation, molecular ecology and metabolic herbicide resistance. This study produced a reference transcriptome for A. spica-venti from different tissues (leaf, root, stem) and various growth stages (seed at phenological stages 05, 07, 08, 09)...
February 6, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28159792/molecular-characterization-and-phylogenetic-analysis-of-two-novel-fungal-phenolic-udp-glycosyltransferases-from-absidia-coerulea-and-rhizopus-japonicus
#18
Kebo Xie, Xiaoxiang Dou, Ridao Chen, Dawei Chen, Cheng Fang, Zhiyan Xiao, Jungui Dai
: In the present study, two novel phenolic UDP-glycosyltransferases (P-UGTs), UGT58A1 and UGT59A1, which can transfer sugar moieties from active donors to phenolic acceptors to generate corresponding glycosides, were identified from the fungi kingdom. UGT58A1 (from Absidia coerulea) and UGT59A1 (from Rhizopus japonicas) share a low degree of homology with known UGTs from animals, plants, bacteria, and virus. These two P-UGTs are membrane-bound proteins with an N-terminal signal peptide and a transmembrane domain at the C-terminus...
February 3, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28154177/glycosyltransferase-st6gal-i-protects-tumor-cells-against-serum-growth-factor-withdrawal-by-enhancing-survival-signaling-and-proliferative-potential
#19
Colleen M Britain, Kaitlyn A Dorsett, Susan L Bellis
A hallmark of cancer cells is the ability to survive and proliferate when challenged with stressors such as growth factor insufficiency. In the current study we report a novel glycosylation-dependent mechanism that protects tumor cells from serum growth factor withdrawal. Results herein suggest that the ST6Gal-I sialyltransferase, which is upregulated in numerous cancers, promotes the survival of serum-starved cells. Using ovarian and pancreatic cancer cell models with ST6Gal-I overexpression or knockdown, we find that serum-starved cells with high ST6Gal-I levels exhibit increased activation of pro-survival signaling molecules including pAkt, p-p70S6K and pNFkB...
January 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28148688/extl3-mutations-cause-skeletal-dysplasia-immune-deficiency-and-developmental-delay
#20
Stefano Volpi, Yasuhiro Yamazaki, Patrick M Brauer, Ellen van Rooijen, Atsuko Hayashida, Anne Slavotinek, Hye Sun Kuehn, Maja Di Rocco, Carlo Rivolta, Ileana Bortolomai, Likun Du, Kerstin Felgentreff, Lisa Ott de Bruin, Kazutaka Hayashida, George Freedman, Genni Enza Marcovecchio, Kelly Capuder, Prisni Rath, Nicole Luche, Elliott J Hagedorn, Antonella Buoncompagni, Beryl Royer-Bertrand, Silvia Giliani, Pietro Luigi Poliani, Luisa Imberti, Kerry Dobbs, Fabienne E Poulain, Alberto Martini, John Manis, Robert J Linhardt, Marita Bosticardo, Sergio Damian Rosenzweig, Hane Lee, Jennifer M Puck, Juan Carlos Zúñiga-Pflücker, Leonard Zon, Pyong Woo Park, Andrea Superti-Furga, Luigi D Notarangelo
We studied three patients with severe skeletal dysplasia, T cell immunodeficiency, and developmental delay. Whole-exome sequencing revealed homozygous missense mutations affecting exostosin-like 3 (EXTL3), a glycosyltransferase involved in heparan sulfate (HS) biosynthesis. Patient-derived fibroblasts showed abnormal HS composition and altered fibroblast growth factor 2 signaling, which was rescued by overexpression of wild-type EXTL3 cDNA. Interleukin-2-mediated STAT5 phosphorylation in patients' lymphocytes was markedly reduced...
February 1, 2017: Journal of Experimental Medicine
keyword
keyword
14746
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"