keyword
MENU ▼
Read by QxMD icon Read
search

Glycosyltransferase

keyword
https://www.readbyqxmd.com/read/28729954/clinical-streptococcal-isolates-distinct-from-streptococcus-pneumoniae-but-containing-the-%C3%AE-glucosyltransferase-tts-gene-and-expressing-serotype-37-capsular-polysaccharide
#1
Carmen L Sheppard, Georgia Kapatai, Karen Broughton, Ulf Schaefer, Matthew Hannah, David J Litt, Norman K Fry
The major virulence factor of the pneumococcus, and target for conjugate vaccines, is the polysaccharide capsule, which is usually encoded by the highly variable cps locus. Serotype 37 is an unusual pneumococcal type in which the single β-glucosyltransferase gene responsible for serotype capsule production (tts) is located outside of the capsular operon region. Using a previously described automated whole genome sequence (WGS)-based serotyping bioinformatics tool, PneumoCaT, we identified and investigated seven clinical isolates (three from blood cultures) of non-pneumococcal streptococci containing a highly homologous tts and included them in a study panel of 20 isolates which included a 11 further clinical isolates of S...
2017: PeerJ
https://www.readbyqxmd.com/read/28725058/identification-of-a-flavonoid-glucosyltransferase-involved-in-7-oh-site-glycosylation-in-tea-plants-camellia-sinensis
#2
Xinlong Dai, Juhua Zhuang, Yingling Wu, Peiqiang Wang, Guifu Zhao, Yajun Liu, Xiaolan Jiang, Liping Gao, Tao Xia
Flavonol glycosides, which are often converted from aglycones in a process catalyzed by UDP-glycosyltransferases (UGTs), play an important role for the health of plants and animals. In the present study, a gene encoding a flavonoid 7-O-glycosyltransferase (CsUGT75L12) was identified in tea plants. Recombinant CsUGT75L12 protein displayed glycosyltransferase activity on the 7-OH position of multiple phenolic compounds. In relative comparison to wild-type seeds, the levels of flavonol-glucosides increased in Arabidopsis seeds overexpressing CsUGT75L12...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28704793/genome-and-transcriptome-wide-analyses-of-cellulose-synthase-gene-superfamily-in-soybean
#3
Muhammad Amjad Nawaz, Hafiz Mamoon Rehman, Faheem Shehzad Baloch, Babar Ijaz, Muhammad Amjad Ali, Iqrar Ahmad Khan, Jeong Dong Lee, Gyuhwa Chung, Seung Hwan Yang
The plant cellulose synthase gene superfamily belongs to the category of type-2 glycosyltransferases, and is involved in cellulose and hemicellulose biosynthesis. These enzymes are vital for maintaining cell-wall structural integrity throughout plant life. Here, we identified 78 putative cellulose synthases (CS) in the soybean genome. Phylogenetic analysis against 40 reference Arabidopsis CS genes clustered soybean CSs into seven major groups (CESA, CSL A, B, C, D, E and G), located on 19 chromosomes (except chromosome 18)...
April 29, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28701886/overexpression-of-ginseng-ugt72al1-causes-organ-fusion-in-the-axillary-leaf-branch-of-arabidopsis
#4
Ngoc Quy Nguyen, Ok Ran Lee
BACKGROUND: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown. METHODS: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28701702/the-influence-of-postharvest-uv-c-treatment-on-anthocyanin-biosynthesis-in-fresh-cut-red-cabbage
#5
Jie Wu, Wen Liu, Li Yuan, Wen-Qiang Guan, Charles S Brennan, Yang-Yong Zhang, Jie Zhang, Zhi-Dong Wang
Red cabbage (Brassica oleracea L. var. capitata f. rubra DC.) is a fresh edible vegetable consumed globally that contains high levels of antioxidant compounds including anthocyanins. In this study, fresh-cut red cabbage was treated with different Ultraviolet-C (UV-C) dosages. Fifteen cyanidin derivatives were observed in UV-C treated fresh-cut red cabbage; four of these were anthocyanins absent in control samples. The optimum dose of UV-C for enhancing total anthocyanin content in fresh-cut red cabbage was 3...
July 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28699515/cell-wall-associated-factors-of-mycobacterium-tuberculosis-as-major-virulence-determinants-current-perspectives-in-drugs-discovery-and-design
#6
Gurdyal Singh, Arbind Kumar, Pratibha Maan, Jagdeep Kaur
Mycobacteria genus is responsible for deadly diseases like tuberculosis and leprosy. Cell wall of bacteria belonging to this genus is unique in many ways. It plays a major role in pathogenesis and intracellular survival inside the host. In intracellular pathogens, their cell wall acts as molecular shield and interacts with host cell milieu to modulate host defense responses. Unique role of cell wall associated factors that play crucial roles in the biosynthesis of mycobacterial cell wall components like Antigen 85 complex, Glycosyltransferases (GTs), LM (lipomannan) and LAM (lipoarabinomannan), mAGP Complex, lipolytic enzyme have been categorically documented...
July 11, 2017: Current Drug Targets
https://www.readbyqxmd.com/read/28698510/genome-sequence-of-pseudomonas-stutzeri-273-and-identification-of-the-exopolysaccharide-eps273-biosynthesis-locus
#7
Shimei Wu, Rikuan Zheng, Zhenxia Sha, Chaomin Sun
Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide 273 (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P.stutzeri 273 was sequenced and the genome contained a circular 5.03 Mb chromosome. With extensive analysis of the genome, a genetic locus containing 18 genes was predicted to be involved in the biosynthesis of EPS273. In order to confirm this prediction, two adjacent genes (eps273-H and eps273-I) encoding glycosyltransferases and one gene (eps273-O) encoding tyrosine protein kinase within the genetic locus were deleted and biosynthesis of EPS273 was checked in parallel...
July 10, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28698248/ganglioside-glycosyltransferases-are-s-acylated-at-conserved-cysteine-residues-involved-in-homodimerization
#8
Sabrina Chumpen Ramirez, Fernando M Ruggiero, Jose Luis Daniotti, Javier Valdez Taubas
Ganglioside glycosyltransferases (GGTs) are type-II membrane proteins bearing a short N-terminal cytoplasmic tail, a transmembrane domain (TMD), and a lumenal catalytic domain. The expression and activity of these enzymes largely determine the quality of the glycolipids that decorate mammalian cells membranes. Many glycosyltransferases are themselves glycosylated and this is important for their proper localisation, but few if any other post-translational modifications of these proteins have been reported. Here we show that the GGTs, ST3Gal-V, ST8Sia-I, and β4GalNAcT-I are S-acylated at conserved cysteine residues located close the cytoplasmic border of their TMDs...
July 11, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28695648/three-glycosylated-serine-rich-repeat-proteins-play-a-pivotal-role-in-adhesion-and-colonization-of-the-pioneer-commensal-bacterium-streptococcus-salivarius
#9
Benoit Couvigny, Nicolas Lapaque, Lionel Rigottier-Gois, Alain Guillot, Sophie Chat, Thierry Meylheuc, Saulius Kulakauskas, Manfred Rohde, Michel-Yves Mistou, Pierre Renault, Joel Doré, Romain Briandet, Pascale Serror, Eric Guédon
Bacterial adhesion is a critical step for colonization of the host. The pioneer colonizer and commensal bacterium of the human gastrointestinal tract, Streptococcus salivarius, has strong adhesive properties but the molecular determinants of this adhesion remain uncharacterized. Serine-rich repeat (SRR) glycoproteins are a family of adhesins that fulfill an important role in adhesion. In general, Gram-positive bacterial genomes have a unique SRR glycoprotein-encoding gene. We demonstrate that S. salivarius expresses three large and glycosylated surface-exposed proteins - SrpA, SrpB and SrpC - that show characteristics of SRR glycoproteins and are secreted through the accessory SecA2/Y2 system...
July 11, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28693426/evolution-gene-expression-profiling-and-3d-modeling-of-csld-proteins-in-cotton
#10
Yanpeng Li, Tiegang Yang, Dandan Dai, Ying Hu, Xiaoyang Guo, Hongxia Guo
BACKGROUND: Among CESA-like gene superfamily, the cellulose synthase-like D (CSLD) genes are most similar to cellulose synthase genes and have been reported to be involved in tip-growing cell and stem development. However, there has been no genome-wide characterization of this gene subfamily in cotton. We thus sought to analyze the evolution and functional characterization of CSLD proteins in cotton based on fully sequenced cotton genomes. RESULTS: A total of 23 full-length CSLD proteins were identified in Gossypium raimondii, Gossypium arboreum and Gossypium hirsutum...
July 10, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/28690624/transcriptome-wide-identification-of-novel-uv-b-and-light-modulated-flavonol-pathway-genes-controlled-by-vvimybf1
#11
Stefan Czemmel, Janine Höll, Rodrigo Loyola, Patricio Arce-Johnson, José Antonio Alcalde, José Tomás Matus, Jochen Bogs
Flavonols constitute a group of flavonoids with important photoprotective roles in plants. In addition, flavonol content and composition greatly influences fruit quality. We previously demonstrated that the grapevine R2R3-MYB transcription factor (TF) VviMYBF1 promotes flavonol accumulation by inducing the expression of flavonol synthase (VviFLS1/VviFLS4), a key step of the initial flavonol pathway. Despite this, gene networks underlying flavonol modification in grapevine including both structural and regulatory genes remain poorly understood...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28690282/targeted-next-generation-sequencing-newly-identifies-mutations-in-exostosin-1-and-exostosin-2-genes-of-patients-with-multiple-osteochondromas
#12
Xiaoyan Guo, Mingrui Lin, Tengfei Shi, Wei Yan, Wenxu Chen
Multiple osteochondromas (MO) is one of the most common benign bone tumors in humans with an autosomal dominant hereditary mode. MO is a genetic heterogeneity disease with variable number and size of osteochondromas, as well as changeable number and location of diseased bones. Mutations in Exostosin-1/Exostosin-2 (EXT1/EXT2) genes are the main molecular basis of MO. EXT1 and EXT2 genes encode exostosin 1 and exostosin 2, respectively, both of which are transmembrane glycosyltransferases that elongate the chains of heparin sulfate (HS) at HS proteoglycans (HSPGs)...
2017: Tohoku Journal of Experimental Medicine
https://www.readbyqxmd.com/read/28685097/restructuring-of-enterococcus-faecalis-biofilm-architecture-in-response-to-antibiotic-induced-stress
#13
Jennifer L Dale, Jennifer L Nilson, Aaron M T Barnes, Gary M Dunny
Bacterial biofilms are intrinsically resistant to antimicrobial treatment, which contributes to microbial persistence in clinical infections. Enterococcus faecalis is an opportunistic pathogen that readily forms biofilms and is the most prevalent enterococcal species identified in healthcare-associated infections. Since intrinsic resistance to multiple antibiotics is common for enterococci, and antibiotic resistance is elevated in biofilm populations, it is imperative to understand the mechanisms involved. Previously, we identified two glycosyltransferase genes whose disruption resulted in impaired nascent biofilm formation in the presence of antibiotic concentrations subinhibitory for parent growth and biofilm formation...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/28683920/computational-glycobiology-mechanistic-studies-of-carbohydrate-active-enzymes-and-implication-for-inhibitor-design
#14
Andrew P Montgomery, Kela Xiao, Xingyong Wang, Danielle Skropeta, Haibo Yu
Carbohydrate-active enzymes (CAZymes) are families of essential and structurally related enzymes, which catalyze the creation, modification, and degradation of glycosidic bonds in carbohydrates to maintain essentially all kingdoms of life. CAZymes play a key role in many biological processes underpinning human health and diseases (e.g., cancer, diabetes, Alzheimer's diseases, AIDS) and have thus emerged as important drug targets in the fight against pathogenesis. The realization of the full potential of CAZymes remains a significant challenge, relying on a deeper understanding of the molecular mechanisms of catalysis...
2017: Advances in Protein Chemistry and Structural Biology
https://www.readbyqxmd.com/read/28678517/substrate-preference-and-interplay-of-fucosyltransferase%C3%A2-8-and-n-acetylglucosaminyltransferases
#15
Tzu-Hao Tseng, Tzu-Wen Lin, Chien-Yu Chen, Chein-Hung Chen, Jung-Lee Lin, Tsui-Ling Hsu, Chi-Huey Wong
The core fucosylation of N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is involved in various biological functions, such as protein function, cancer progression, and postnatal development. The substrate specificity of FUT8 toward bi-antennary N-glycans has been reported, but it is unclear with regard to tri-antennary and tetra-antennary glycans. Here, we examined the specificity and activity of human FUT8 toward tri- and tetra-antennary N-glycans in the forms of glycopeptides...
July 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28670741/structural-mutagenic-and-in-silico-studies-of-xyloglucan-fucosylation-in-arabidopsis-thaliana-suggest-a-water-mediated-mechanism
#16
Breeanna R Urbanowicz, Vivek S Bharadwaj, Markus Alahuhta, Maria J Peña, Vladimir V Lunin, Yannick J Bomble, Shuo Wang, Jeong-Yeh Yang, Sami T Tuomivaara, Michael E Himmel, Kelley W Moremen, William S York, Michael F Crowley
The mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely due to the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase-1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2-fucosyl residues to xyloglucan side chains - a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28667643/upscale-production-of-a-recombinant-cyclodextrin-glycosyltransferase-from-paenibacillus-macerans-in-escherichia-coli
#17
Yi-Nan Yang, Wen-Xin Shan, Pi-Wu Wang
Cyclodextrin glucanotransferase (CGTase) is an important enzyme with multiple functions in starch utilization. In the present study, a fermentation process for the production of CGTase from Escherichia coli harboring the recombinant plasmid pET28b(+)-CGTase was investigated and optimized. The optimal fermentation and expression conditions were 10.0 g/L glycerol, 20.0 g/L tryptone, and 10.0 g/L yeast extract with an initial pH of 7.0, an IPTG concentration of 0.1 mM and an induction temperature of 28 °C for 10 h...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28667570/molecular-cloning-expression-and-characterization-of-oenococcus-oeni-priming-glycosyltransferases
#18
Maria Dimopoulou, Olivier Claisse, Lucie Dutilh, Cécile Miot-Sertier, Patricia Ballestra, Patrick M Lucas, Marguerite Dols-Lafargue
Oenococcus oeni is the main bacterial species that drives malolactic fermentation in wine. Most O. oeni strains produce capsular exopolysaccharides (EPS) that may contribute to protect them in the wine hostile environment. In O. oeni genome sequences, several genes are predicted to encode priming glycosyltransferases (pGTs). These enzymes are essential for EPS formation as they catalyze the first biosynthetic step through the formation of a phosphoanhydride bond between a hexose-1-phosphate and a lipid carrier undecaprenyl phosphate...
August 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28666720/clinicopathological-implications-to-micropapillary-bladder-urothelial-carcinoma-of-the-presence-of-sialyl-lewis-x-decorated-mucin-1-in-stroma-facing-membranes
#19
Tomochika Shinagawa, Hitomi Hoshino, Minekatsu Taga, Yasuhiro Sakai, Yoshiaki Imamura, Osamu Yokoyama, Motohiro Kobayashi
OBJECTIVES: Bladder urothelial carcinoma (UC) comprises more than 90% of all bladder cancers. Among several UC variants, micropapillary UC (MPUC) is a rare one with high potential for lymphovascular invasion and subsequent lymph node metastasis. Histologically, MPUC is characterized by the presence of small papillary carcinoma cell clusters surrounded by lacunar spaces. Immunohistochemically, the outer circumference of these clusters, that is, the stroma-facing membrane of carcinoma cells, is reportedly almost invariably positive for mucin 1 (MUC1) protein and to a lesser extent for sialyl Lewis X (sLeX) carbohydrates; however, the clinicopathological implications of these expression patterns have not been fully investigated...
June 27, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28666318/autologous-intramuscular-transplantation-of-engineered-satellite-cells-induces-exosome-mediated-systemic-expression-of-fukutin-related-protein-and-rescues-disease-phenotype-in-a-murine-model-of-limb-girdle-muscular-dystrophy-type-2i
#20
Paola Frattini, Chiara Villa, Francesca De Santis, Mirella Meregalli, Marzia Belicchi, Silvia Erratico, Pamela Bella, Manuela Teresa Raimondi, Qilong Lu, Yvan Torrente
α-Dystroglycanopathies are a group of muscular dystrophies characterized by α-DG hypoglycosylation and reduced extracellular ligand-binding affinity. Among other genes involved in the α-DG glycosylation process, fukutin related protein (FKRP) gene mutations generate a wide range of pathologies from mild limb girdle muscular dystrophy 2I (LGMD2I), severe congenital muscular dystrophy 1C (MDC1C), to Walker-Warburg Syndrome and Muscle-Eye-Brain disease. FKRP gene encodes for a glycosyltransferase that in vivo transfers a ribitol phosphate group from a CDP -ribitol present in muscles to α-DG, while in vitro it can be secreted as monomer of 60kDa...
June 29, 2017: Human Molecular Genetics
keyword
keyword
14746
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"