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https://www.readbyqxmd.com/read/28721831/transient-production-of-recombinant-pharmaceutical-proteins-in-plants-evolution-and-perspectives
#1
Lilya Kopertekh, Joachim Schiemann
During the last two decades the production of pharmaceutical proteins in plants evolved from proof of concept to established technology adopted by several biotechnological companies. This progress is particularly based on intensive research starting stable genetic transformation and moving to transient expression. Due to its advantages in yield and speed of protein production transient expression platforms became leading plant-based manufacturing technology. Current transient expression methods rely on Agrobacterium-mediated delivery of expression vectors into plant cells...
July 18, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28720727/impact-of-paracoccin-gene-silencing-on-paracoccidioides-brasiliensis-virulence
#2
Fabrício F Fernandes, Aline F Oliveira, Taise N Landgraf, Cristina Cunha, Agostinho Carvalho, Patrícia E Vendruscolo, Relber A Gonçales, Fausto Almeida, Thiago A da Silva, Fernando Rodrigues, Maria Cristina Roque-Barreira
Among the endemic deep mycoses in Latin America, paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the Paracoccidioides genus, is a major cause of morbidity. Disease development and its manifestations are associated with both host and fungal factors. Concerning the latter, several recent studies have employed the methodology of gene modulation in P. brasiliensis using antisense RNA (AsRNA) and Agrobacterium tumefaciens-mediated transformation (ATMT) to identify proteins that influence fungus virulence...
July 18, 2017: MBio
https://www.readbyqxmd.com/read/28718508/development-of-ifox-hunting-as-a-functional-genomics-tool-and-demonstration-of-its-use-to-identify-early-senescence-related-genes-in-the-polyploid-brassica-napus
#3
Juan Ling, Renjie Li, Chinedu Charles Nwafor, Junluo Cheng, Maoteng Li, Qing Xu, Jian Wu, Lu Gan, Qingyong Yang, Chao Liu, Ming Chen, Yongming Zhou, Edgar B Cahoon, Chunyu Zhang
Functional genomics studies of many polyploid crops, including rapeseed (Brassica napus) are constrained by limited tool sets. Here we report development of a gain-of-function platform, termed "iFOX (inducible Full-length cDNA OvereXpressor gene)-Hunting", for inducible expression of B. napus seed cDNAs in Arabidopsis. A Gateway-compatible plant gene expression vector containing a methoxyfenozide-inducible constitutive promoter for transgene expression was developed. This vector was used for cloning of random cDNAs from developing B...
July 17, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28715654/generation-of-stable-infectious-clones-of-plant-viruses-by-using-rhizobium-radiobacter-for-both-cloning-and-inoculation
#4
Decai Tuo, Lanlan Fu, Wentao Shen, Xiaoying Li, Peng Zhou, Pu Yan
A novel Rhizobium radiobacter (synonym Agrobacterium tumefaciens)-mediated approach was developed to generate stable infectious clones of plant viruses. This method uses R. radiobacter for both cloning and inoculation of infectious clones, bypassing the requirement of cloning in E. coli to avoid the instability. Only three steps are included in this method: (i) construct viral genome-encoding plasmids in vitro by one-step Gibson assembly; (ii) transform the assembled DNA products into R. radiobacter; (iii) inoculate plants with the R...
July 14, 2017: Virology
https://www.readbyqxmd.com/read/28713400/overexpression-of-e3-ubiquitin-ligase-gene-adbil-contributes-to-resistance-against-chilling-stress-and-leaf-mold-disease-in-tomato
#5
Shuangchen Chen, Hongjiao Zhao, Mengmeng Wang, Jidi Li, Zhonghong Wang, Fenghua Wang, Airong Liu, Golam J Ahammed
Ubiquitination is a common regulatory mechanism, playing a critical role in diverse cellular and developmental processes in eukaryotes. However, a few reports on the functional correlation between E3 ubiquitin ligases and reactive oxygen species (ROS) or reactive nitrogen species (RNS) metabolism in response to stress are currently available in plants. In the present study, the E3 ubiquitin ligase gene AdBiL (Adi3 Binding E3 Ligase) was introduced into tomato line Ailsa Craig via Agrobacterium-mediated method...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28709614/generation-of-recombinant-protein-shells-of-johnson-grass-chlorotic-stripe-mosaic-virus-in-tobacco-plants-and-their-use-as-drug-carrier
#6
E Alemzadeh, K Izadpanah, F Ahmadi
The development and use of virus-like particles (VLPs) is a growing field with a powerful potential in generation of nanoparticles. In the present study we have attempted to generate and use empty shells of Johnson grass chlorotic stripe mosaic virus (JgCSMV, a member of the genus Aureusvirus, family Tombusviridae) as VLP nanoparticles for drug loading. In order to successfully produce recombinant JgCSMV-derived VLPs, we followed an approach based on cloning of the JgCSMV CP gene into pBI121 vectorand introduction of the latter into Agrobacterium rhizogenes and transformation of tobacco cells for coat protein expression...
July 11, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28705394/melanization-and-ageing-are-not-drawbacks-for-successful-agro-transformation-of-dark-septate-endophytes
#7
Charlotte Berthelot, Yoann Perrin, Corinne Leyval, Damien Blaudez
Dark septate endophytes (DSEs) are melanin-enriched ascomycetous fungi that are abundant in stressed environments. However, little is known about their physiology and metabolism, and DSE genes have not been functionally characterized yet. Therefore developing molecular genetic tools to investigate the biological function of genes of interest in DSEs is of major significance. We investigated Agrobacterium tumefaciens-mediated transformation (ATMT) efficiency in eight DSE strains belonging to Cadophora sp., Cadophora malorum, Leptodontidium sp...
August 2017: Fungal Biology
https://www.readbyqxmd.com/read/28701886/overexpression-of-ginseng-ugt72al1-causes-organ-fusion-in-the-axillary-leaf-branch-of-arabidopsis
#8
Ngoc Quy Nguyen, Ok Ran Lee
BACKGROUND: Glycosylation of natural compounds increases the diversity of secondary metabolites. Glycosylation steps are implicated not only in plant growth and development, but also in plant defense responses. Although the activities of uridine-dependent glycosyltransferases (UGTs) have long been recognized, and genes encoding them in several higher plants have been identified, the specific functions of UGTs in planta remain largely unknown. METHODS: Spatial and temporal patterns of gene expression were analyzed by quantitative reverse transcription (qRT)-polymerase chain reaction (PCR) and GUS histochemical assay...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28701884/cis-prenyltransferase-interacts-with-a-nogo-b-receptor-homolog-for-dolichol-biosynthesis-in-panax-ginseng-meyer
#9
Ngoc Quy Nguyen, Sang-Choon Lee, Tae-Jin Yang, Ok Ran Lee
BACKGROUND: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed...
July 2017: Journal of Ginseng Research
https://www.readbyqxmd.com/read/28698212/erratum-for-the-research-article-agrobacterium-counteracts-host-induced-degradation-of-its-effector-f-box-protein-by-s-magori-and-v-citovsky
#10
(no author information available yet)
No abstract text is available yet for this article.
July 11, 2017: Science Signaling
https://www.readbyqxmd.com/read/28694815/co-overexpression-of-the-constitutively-active-form-of-osbzip46-and-aba-activated-protein-kinase-sapk6-improves-drought-and-temperature-stress-resistance-in-rice
#11
Yu Chang, Ba Hoanh Nguyen, Yongjun Xie, Benze Xiao, Ning Tang, Wenliu Zhu, Tongmin Mou, Lizhong Xiong
Drought is one of the major abiotic stresses threatening rice (Oryza sativa) production worldwide. Drought resistance is controlled by multiple genes, and therefore, a multi-gene genetic engineering strategy is theoretically useful for improving drought resistance. However, the experimental evidence for such a strategy is still lacking. In this study, a few drought-responsive genes from rice were assembled by a multiple-round site-specific assembly system, and the constructs were introduced into the rice cultivar KY131 via Agrobacterium-mediated transformation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28688010/quorum-sensing-molecules-production-by-nosocomial-and-soil-isolates-acinetobacter-baumannii
#12
Demet Erdönmez, Abbas Yousefi Rad, Nilüfer Aksöz
Acinetobacter species remain alive in hospitals on various surfaces, both dry and moist, forming an important source of hospital infections. These bacteria are naturally resistant to many antibiotic classes. Although the role of the quorum sensing system in regulating the virulence factors of Acinetobacter species has not been fully elucidated, it has been reported that they play a role in bacterial biofilm formation. The biofilm formation helps them to survive under unfavorable growth conditions and antimicrobial treatments...
July 7, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28687763/a-tyrosine-aminotransferase-involved-in-rosmarinic-acid-biosynthesis-in-prunella-vulgaris-l
#13
Mei Ru, Kunru Wang, Zhenqing Bai, Liang Peng, Shaoxuan He, Yong Wang, Zongsuo Liang
Rosmarinic acid (RA) and its derivants are medicinal compounds that comprise the active components of several therapeutics. We isolated and characterised a tyrosine aminotransferase of Prunella vulgaris (PvTAT). Deduced PvTAT was markedly homologous to other known/putative plant TATs. Cytoplasmic localisation of PvTAT was observed in tobacco protoplasts. Recombinantly expressed and purified PvTAT had substrates preference for L-tyrosine and phenylpyruvate, with apparent K m of 0.40 and 0.48 mM, and favoured the conversion of tyrosine to 4-hydroxyphenylpyruvate...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28684751/genomic-and-transcriptomic-analyses-of-agrobacterium-tumefaciens-s33-reveal-the-molecular-mechanism-of-a-novel-hybrid-nicotine-degrading-pathway
#14
Haiyan Huang, Wenjun Yu, Rongshui Wang, Huili Li, Huijun Xie, Shuning Wang
Agrobacterium tumefaciens S33 is able to degrade nicotine via a novel hybrid of the pyridine and pyrrolidine pathways. It can be utilized to remove nicotine from tobacco wastes and transform nicotine into important functionalized pyridine precursors for some valuable drugs and insecticides. However, the molecular mechanism of the hybrid pathway is still not completely clear. Here we report the genome analysis of strain S33 and its transcriptomes grown in glucose-ammonium medium and nicotine medium. The complete gene cluster involved in nicotine catabolism was found to be located on a genomic island composed of genes functionally similar but not in sequences to those of the pyridine and pyrrolidine pathways, as well as genes encoding plasmid partitioning and replication initiation proteins, conjugal transfer proteins and transposases...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28681159/selectable-marker-independent-transformation-of-recalcitrant-maize-inbred-b73-and-sorghum-p898012-mediated-by-morphogenic-regulators-baby-boom-and-wuschel2
#15
Muruganantham Mookkan, Kimberly Nelson-Vasilchik, Joel Hague, Zhanyuan J Zhang, Albert P Kausch
Discriminatory co-expression of maize BBM and WUS transcriptional factor genes promoted somatic embryogenesis and efficient Agrobacterium -mediated transformation of recalcitrant maize inbred B73 and sorghum P898012 genotypes without use of a selectable marker gene. The use of morphogenic regulators to overcome barriers in plant transformation is a revolutionary breakthrough for basic plant science and crop applications. Current standard plant transformation systems are bottlenecks for genetic, genomic, and crop improvement studies...
July 5, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28676963/knocking-out-of-carotenoid-catabolic-genes-in-rice-fails-to-boost-carotenoid-accumulation-but-reveals-a-mutation-in-strigolactone-biosynthesis
#16
Xiaoyu Yang, Lei Chen, Junxian He, Weichang Yu
Targeted mutations in five carotenoid catabolism genes failed to boost carotenoid accumulation in rice seeds, but produced dwarf and high tillering mutants when OsCCD7 gene was knocked out. Carotenoids play an important role in human diet as a source of vitamin A. Rice is a major staple food in Asia, but does not accumulate carotenoids in the endosperm because of the low carotenoid biosynthesis or the degradation in metabolism. In this study, the CRISPR/Cas9 system was investigated in the targeted knockout of five rice carotenoid catabolic genes (OsCYP97A4, OsDSM2, OsCCD4a, OsCCD4b and OsCCD7) and in an effort to increase β-carotene accumulation in rice endosperm...
July 4, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/28676913/co-expression-of-beta-glucosidase-and-laccase-in-trichoderma-reesei-by-random-insertion-with-enhanced-filter-paper-activity
#17
Ruowen Wang, Jing Yang, Guoqing Zhang, Yapeng Chao, Zhimin Li, Qin Ye, Shijun Qian
Trichoderma reesei strain Rut-C30 was modified with enhanced beta-glycosidase (BGL) activity to balance the cellulase system and generated laccase (LAC) protein for lignin degradation. Initially, the binary plasmid p1300-w1 was constructed to express T. reesei bgl2 under the control of promoter P pki and T-nos terminator. Random insertion was performed via Agrobacterium tumefaciens-mediated transformation. A total of 353 mutants were obtained, and 34PTrb2 was exceptionally stable with increased FPA and BGL activity after screening for extracellular enzyme activity...
August 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28673485/high-resolution-crystal-structure-of-a-fluoride-inhibited-organophosphate-degrading-metallohydrolase
#18
Christopher Selleck, Luke W Guddat, David L Ollis, Gerhard Schenk, Marcelo Monteiro Pedroso
Metal ion-dependent, organophosphate-degrading enzymes (OP hydrolases) have received increasing attention due to their ability to degrade and thus detoxify commonly used pesticides and nerve agents such as sarin and VX. These enzymes thus garner strong potential as bioremediators. The OP hydrolase from Agrobacterium radiobacter (OpdA) is one of the most efficient members of this group of enzymes. Previous studies have indicated that the choice of the hydrolysis-initiating nucleophile may depend on the pH of the reaction, with a metal ion-bridging hydroxide being preferred at lower pH (i...
June 27, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28670824/a-3d-digital-atlas-of-the-nicotiana-tabacum-root-tip-and-its-use-to-investigate-changes-in-the-root-apical-meristem-induced-by-the-agrobacterium-6b-oncogene
#19
Taras Pasternak, Thomas Haser, Thorsten Falk, Olaf Ronneberger, Klaus Palme, Léon Otten
Using the intrinsic Root Coordinate System (iRoCS) Toolbox, a digital atlas at cellular resolution has been constructed for Nicotiana tabacum roots. Mitotic cells and cells labeled for DNA replication with 5-ethynyl-2'-deoxyuridine (EdU) were mapped. The results demonstrate that iRoCS analysis can be applied to thicker roots than those of Arabidopsis thaliana without histological sectioning. A three-dimensional (3-D) analysis of the root tip showed that tobacco roots undergo several irregular periclinal and tangential divisions...
July 3, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28670322/a-genetic-transformation-method-for-cadmium-hyperaccumulator-sedum-plumbizincicola-and-non-hyperaccumulating-ecotype-of-sedum-alfredii
#20
Huan Liu, Haixia Zhao, Longhua Wu, Wenzhong Xu
The present study demonstrates the development of an Agrobacterium-mediated genetic transformation method for species of the Sedum genus, which includes the Cd/Zn hyperaccumulator Sedum plumbizincicola and the non-hyperaccumulating ecotype of S. alfredii. Multiple shoots were induced from stem nodes of two Sedum plants using Murashige and Skoog (MS) medium containing 0.1 mg/L cytokinin 6-benzyladenine (6-BA) and 1.0 mg/L auxin 1-naphthaleneacetic acid (NAA). The shoot primordia were used as direct targets for Agrobacterium infection...
2017: Frontiers in Plant Science
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