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Yupeng Cai, Li Chen, Xiujie Liu, Chen Guo, Shi Sun, Cunxiang Wu, Bingjun Jiang, Tianfu Han, Wensheng Hou
Flowering is an indication of the transition from vegetative growth to reproductive growth and has considerable effects on the life cycle of soybean (Glycine max). In this study, we employed the CRISPR/Cas9 system to specifically induce targeted mutagenesis of GmFT2a, an integrator in the photoperiod flowering pathway in soybean. The soybean cultivar Jack was transformed with three sgRNA/Cas9 vectors targeting different sites of endogenous GmFT2a via Agrobacterium tumefaciens-mediated transformation. Site-directed mutations were observed at all targeted sites by DNA sequencing analysis...
May 16, 2017: Plant Biotechnology Journal
Jeum Kyu Hong, In Sun Hwang, Byung Kook Hwang
Pepper leucine-rich repeat protein (CaLRR1) interacts with defense response proteins to regulate plant cell death and immunity. This review highlights the current understanding of the molecular functions of CaLRR1 and its interactor proteins. Plant cell death and immune responses to microbial pathogens are controlled by complex and tightly regulated molecular signaling networks. Xanthomonas campestris pv. vesicatoria (Xcv)-inducible pepper (Capsicum annuum) leucine-rich repeat protein 1 (CaLRR1) serves as a molecular marker for plant cell death and immunity signaling...
May 15, 2017: Planta
Aref Takiden, Francisco Velazquez-Escobar, Jovan Dragelj, Anna Lena Woelke, Ernst-Walter Knapp, Patrick Piwowarski, Franz Bart, Peter Hildebrandt, Maria Andrea Mroginski
Agp1 is a prototypical bacterial phytochrome from Agrobacterium fabrum harboring a biliverdin cofactor which reversibly photoconverts between a red-light-absorbing (Pr) and a far-red-light-absorbing (Pfr) states. The reaction mechanism involves the isomerization of the bilin-chromophore followed by large structural changes of the protein matrix that are coupled to protonation dynamics at the chromophore binding site. Histidines His250 and His280 participate in this process. Although the three-dimensional structure of Agp1 has been solved at high resolution, the precise position of hydrogen atoms and protonation pattern in the chromophore binding pocket has not been investigated yet...
May 2017: Photochemistry and Photobiology
Francisco Velázquez Escobar, David Buhrke, Norbert Michael, Luisa Sauthof, Svea Wilkening, Neslihan N Tavraz, Johannes Salewski, Nicole Frankenberg-Dinkel, Maria Andrea Mroginski, Patrick Scheerer, Thomas Friedrich, Friedrich Siebert, Peter Hildebrandt
Phytochromes are bimodal photoreceptors which, upon light absorption by the tetrapyrrole chromophore, can be converted between a red-absorbing state (Pr) and far-red-absorbing state (Pfr). In bacterial phytochromes, either Pr or Pfr are the thermally stable states, thereby constituting the classes of prototypical and bathy phytochromes, respectively. In this work, we have employed vibrational spectroscopies to elucidate the origin of the thermal stability of the Pfr states in bathy phytochromes. Here, we present the first detailed spectroscopic analysis of RpBphP6 (Rhodopseudomas palustris), which together with results obtained for Agp2 (Agrobacterium tumefaciens) and PaBphP (Pseudomonas aeruginosa) allows identifying common structural properties of the Pfr state of bathy phytochromes, which are (1) a homogenous chromophore structure, (2) the protonated ring C propionic side chain of the chromophore and (3) a retarded H/D exchange at the ring D nitrogen...
May 2017: Photochemistry and Photobiology
Tilman Lamparter, Norbert Krauß, Patrick Scheerer
Agrobacterium fabrum is a widely used model bacterium for gene transfer from pro- to eukaryote, for genetics and metabolism. The phytochrome system of Agrobacterium, encompassing the two phytochromes Agp1 and Agp2, has provided deep insight into phytochrome action in a bacterial organism. This review summarizes recent results on phytochrome evolution, phytochrome regulation of conjugation and plant infection and biochemical studies including the crystal structure of Agp1-PCM, the photosensory core module of Agp1...
May 2017: Photochemistry and Photobiology
Diogo Queirós, Alexandre Fonseca, Simona Rossetti, Luísa S Serafim, Paulo C Lemos
Mixed microbial cultures (MMC) and waste/surplus substrates, as hardwood spent sulfite liquor, are being used to decrease polyhydroxyalkanoates' (PHA) production costs. The process involves two or three steps, being the selection step a crucial one. For the industrial implementation of this strategy, reactor stability in terms of both performance and microbial community presence has to be considered. A long-term operation of a sequencing batch reactor under feast/famine conditions was performed along with microbial community identification/quantification using FISH and DGGE...
May 11, 2017: Journal of Industrial Microbiology & Biotechnology
Jeremiah Olorunjuwon Olugbami, Robert Damoiseaux, Bryan France, Michael A Gbadegesin, Adam Z Stieg, Shivani Sharma, Oyeronke A Odunola, James K Gimzewski
OBJECTIVE: The fatality of cancer is mostly dependent on the possibility of occurrence of metastasis. Thus, if the development of metastasis can be prevented through novel therapeutic strategies targeted against this process, then the success of cancer treatment will drastically increase. In this study, therefore, we evaluated the antimetastatic potentials of an extract of Khaya senegalensis and curcumin on the metastatic liver cell line HepG2, and also assessed the anticancer property of the extract...
May 2017: Journal of Integrative Medicine
Camila Sampaio Mangolim, Thamara Thaiane da Silva, Vanderson Carvalho Fenelon, Adriane do Nascimento, Francielle Sato, Graciette Matioli
Curdlan is a linear polysaccharide composed of glucose units joined by β-(1,3) bonds that possesses unique gelation properties. This study aimed to characterize the structure and evaluate the gelling properties of curdlan produced by Agrobacterium sp. IFO 13140 and its gels, as well as apply it in food. FT-Raman analysis highlighted the structural changes that occurred during the formation of gels, with variations related to the hydrogen bonds and hydrophobic interactions, which occur with the formation of the low-set and high-set gels, respectively...
October 1, 2017: Food Chemistry
Eszter Kapusi, Maria Corcuera-Gómez, Stanislav Melnik, Eva Stoger
Targeted genome editing with the CRISPR/Cas9 system has been used extensively for the selective mutation of plant genes. Here we used CRISPR/Cas9 to disrupt the putative barley (Hordeum vulgare cv. "Golden Promise") endo-N-acetyl-β-D-glucosaminidase (ENGase) gene. Five single guide RNAs (sgRNAs) were designed for different target sites in the upstream part of the ENGase coding region. Targeted fragment deletions were induced by co-bombarding selected combinations of sgRNA with wild-type cas9 using separate plasmids, or by co-infection with separate Agrobacterium tumefaciens cultures...
2017: Frontiers in Plant Science
Mukesh Kumar Awasthi, Zengqiang Zhang, Quan Wang, Feng Shen, Ronghua Li, Dong-Sheng Li, Xiuna Ren, Meijing Wang, Hongyu Chen, Junchao Zhao
In this work, the 16S rRNA gene was used to compare the bacterial diversity at thermophilic stage of six different biochar dosage applied SS composting, while compared without biochar added treatment or control. The results showed that biochar amendment affected the structure and succession of bacteria diversity in different ways, and there were 35 prominent genera among all samples displayed in the species abundance heat-map. Among them, Proteobacteria, Firmicutes and Chloroflexi were the main phyla found in genomic libraries from each treatment...
April 27, 2017: Bioresource Technology
Chunling Wang, Guoqing Lu, Yuqiong Hao, Huiming Guo, Yan Guo, Jun Zhao, Hongmei Cheng
ABP9 , encoding a bZIP transcription factor from maize, enhances tolerance to multiple stresses and may participate in the ABA signaling pathway in transgenic cotton by altering physiological and biochemical processes and stress-related gene expression. Abiotic stresses, such as soil salinity and drought, negatively affect growth, development, and yield in cotton. Gene ABP9, which encodes a bZIP transcription factor, binds to the abscisic acid (ABA)-responsive-element (ABRE2) motif of the maize catalase1 gene...
May 4, 2017: Planta
Hanyu Zhu, Dongmei Liu, Yuanyuan Wang, Danfeng Ren, Liesheng Zheng, Liguo Chen, Aimin Ma
OBJECTIVES: To obtain hydrophobin, a Class I hydrophobin gene, Po.hyd from Pleurotus ostreatus, was transformed into the yeast-like cells of Tremella fuciformis using Agrobacterium tumefaciens. RESULTS: The hydrophobin Po.HYD from P. ostreatus was heterogeneously expressed by the yeast-like cells of T. fuciformis. Plasmids harboring the Po.hyd gene driven by endogenous glyceraldehyde-3-phosphate dehydrogenase promoter were transformed by A. tumefaciens. The integration and expression of the rPo...
May 3, 2017: Biotechnology Letters
Wei-Ting Liu, Peng-Wen Chen, Li-Chi Chen, Chia-Chun Yang, Shu-Yun Chen, GuanFu Huang, Tzu Che Lin, Hsin-Mei Ku, Jeremy J W Chen
KEY MESSAGE: miR319 was identified as a dwarf-inducing gene from Shiokari and its dwarf near isogenic line, and its transgenic rice showed a reduced plant height. This finding reveals the potential application of miR319 in future molecular breeding. It is well known that microRNAs (miRNAs) play important roles in plant physiology, especially in development and stress responses. However, little is known about the role of miRNAs in plant height. In this study, the rice cultivar Shiokari and its dwarf near isogenic line Shiokari-d6 were analysed to identify and characterize plant height-associated miRNAs...
May 3, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Xia An, Jingyu Zhang, Yiwen Liao, Lijun Liu, Dingxiang Peng, Bo Wang
Ramie is an economically important industrial fiber crop widely planted in China, India, and other Southeast Asian and Pacific Rim countries. It plays an important role in China's economy, where ramie farming, industry, and trade provide livelihood support to about five million people. However, poor fiber production resulting from leaf senescence and leaf abscission is a significant problem. In this study, we report the successful production of transgenic ramie plants which delayed leaf senescence and enhanced biomass...
May 2017: FEBS Open Bio
Khuyen Thi Nguyen, Quynh Ngoc Ho, Loc Thi Binh Xuan Do, Linh Thi Dam Mai, Duc-Ngoc Pham, Huyen Thi Thanh Tran, Diep Hong Le, Huy Quang Nguyen, Van-Tuan Tran
Aspergillus oryzae is a filamentous fungus widely used in food industry and as a microbial cell factory for recombinant protein production. Due to the inherent resistance of A. oryzae to common antifungal compounds, genetic transformation of this mold usually requires auxotrophic mutants. In this study, we show that Agrobacterium tumefaciens-mediated transformation (ATMT) method is very efficient for deletion of the pyrG gene in different Aspergillus oryzae wild-type strains to generate uridine/uracil auxotrophic mutants...
June 2017: World Journal of Microbiology & Biotechnology
Gaojie Li, Shiqi Hu, Jingjing Yang, Elizabeth A Schultz, Kurtis Clarke, Hongwei Hou
The semi-aquatic plant Water-Wisteria is suggested as a new model to study heterophylly due to its many advantages and typical leaf phenotypic plasticity in response to environmental factors and phytohormones. Water-Wisteria, Hygrophila difformis (Acanthaceae), is a fast growing semi-aquatic plant that exhibits a variety of leaf shapes, from simple leaves to highly branched compound leaves, depending on the environment. The phenomenon by which leaves change their morphology in response to environmental conditions is called heterophylly...
May 2, 2017: Plant Cell Reports
Fang Tai, Sulian Lv, Ping Jiang, Jinhui Wang, Juanjuan Feng, Yinxin Li
A Salicornia europaea L. in vitro cell transformation system was developed and further applied to SeNHX1 function investigation. The exploration of salt-tolerant genes from halophyte has seriously been limited by the lack of self-dependent transformation system. Here, an Agrobacterium tumefaciens-mediated in vitro cell transformation system of euhalophyte Salicornia europaea L. was developed. Calli derived from hypocotyl of S. europaea were co-cultured for 3 days with Agrobacterium at OD600 ranging from 1...
May 2, 2017: Plant Cell Reports
Qingchang Liu
Sweetpotato is the seventh most important food crop in the world. It is mainly used for human food, animal feed, and for manufacturing starch and alcohol. This crop, a highly heterozygous, generally self-incompatible, outcrossing polyploidy, poses numerous challenges for the conventional breeding. Its productivity and quality are often limited by abiotic and biotic stresses. Gene engineering has been shown to have the great potential for improving the resistance to these stresses as well as the nutritional quality of sweetpotato...
January 2017: Breeding Science
Muhammad Imran, Shaheen Asad, Andre Luiz Barboza, Esteban Galeano, Helaine Carrer, Zahid Mukhtar
Glyphosate quashes the synthesis of 5-enolpyruvylshikimate-3- phosphate synthase (EPSPS) enzyme which intercedes the functioning of shikimate pathway for the production of aromatic amino acids. Herbicide resistant crops are developed using glyphosate insensitive EPSPS gene isolated from Agrobacterium sp. strain CP4, which give farmers a sustainable weed control option. Intentions behind this study were to design and characterize the synthetic herbicide resistant CP4-EPSPS gene in a model plant system and check the effectiveness of transformed tobacco against application of glyphosate...
April 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Marie-Christine Hoffmann, Yvonne Pfänder, Marc Tintel, Bernd Masepohl
Rhodobacter capsulatus synthesizes the high-affinity ABC transporters CysTWA and ModABC to specifically import the chemically related oxyanions sulfate and molybdate, respectively. In addition, R. capsulatus has the low-affinity permease PerO acting as a general oxyanion transporter, whose elimination increases tolerance to molybdate and tungstate. Although widespread in bacteria, the function of PerO-like permeases has not been examined in any other species to date. Here, we present evidence that PerO permeases from the Alphaproteobacteria Agrobacterium tumefaciens, Dinoroseobacter shibae, Rhodobacter sphaeroides, and Sinorhizobium meliloti, and the Gammaproteobacterium Pseudomonas stutzeri functionally substitute for R...
May 1, 2017: Journal of Bacteriology
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