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https://www.readbyqxmd.com/read/28026817/-a-possibility-to-use-the-dna-based-probes-as-internal-standards-for-agilent-technologies-microarray-transcriptomic-analysis
#1
L K Kurbatov, V G Zgoda
Accuracy of the microarray technology results is raised by using the multi-stage normalization of results. One of the principal requirements of such normalization is usage of internal standards. The routine Agilent microarray-based gene expression analysis protocol utilizes a Spike-In Kit during preparation of the samples representing a mixture of RNA fragments in different ratios. RNA probes which were synthesized in vitro conditions could be also used to establish how the magnitude of the fluorescent signal reflects the presence of RNA in the sample...
November 2016: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28004117/a-blood-based-gene-expression-and-signaling-pathway-analysis-to-differentiate-between-high-and-low-grade-gliomas
#2
Stephen N Ponnampalam, Nor Rizan Kamaluddin, Zubaidah Zakaria, Vickneswaran Matheneswaran, Dharmendra Ganesan, Mohammed Saffari Haspani, Mina Ryten, John A Hardy
The aims of the present study were to undertake gene expression profiling of the blood of glioma patients to determine key genetic components of signaling pathways and to develop a panel of genes that could be used as a potential blood-based biomarker to differentiate between high and low grade gliomas, non-gliomas and control samples. In this study, blood samples were obtained from glioma patients, non-glioma and control subjects. Ten samples each were obtained from patients with high and low grade tumours, respectively, ten samples from non-glioma patients and twenty samples from control subjects...
January 2017: Oncology Reports
https://www.readbyqxmd.com/read/28001018/advanced-practice-registered-nurses-final-rule-with-comment-period
#3
(no author information available yet)
The Department of Veterans Affairs (VA) is amending its medical regulations to permit full practice authority of three roles of VA advanced practice registered nurses (APRN) when they are acting within the scope of their VA employment. Certified Registered Nurse Anesthetists (CRNA) will not be included in VA's full practice authority under this final rule, but comment is requested on whether there are access issues or other unconsidered circumstances that might warrant their inclusion in a future rulemaking...
14, 2016: Federal Register
https://www.readbyqxmd.com/read/27978824/unspecific-binding-of-crna-probe-to-plaques-in-two-mouse-models-for-alzheimer-s-disease
#4
Anne Schaarschuch, Christoph Redies, Nicole Hertel
BACKGROUND: Alzheimer's disease (AD) is characterized by the pathological deposition of amyloid-β (Aβ) protein-containing plaques. Microglia and astrocytes are commonly attracted to the plaques by an unknown mechanism that may involve cell adhesion. One cell adhesion family of proteins, the cadherins, are widely expressed in the central nervous system. Therefore, our study was designed to map the expression of cadherins in AD mouse brains. A particular focus was on plaques because diverse mRNA-species were found in plaques and their surrounding area in brains of AD patients...
December 16, 2016: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/27966593/subtype-specific-structural-constraints-in-the-evolution-of-influenza-a-virus-hemagglutinin-genes
#5
Alexander P Gultyaev, Monique I Spronken, Mathilde Richard, Eefje J A Schrauwen, René C L Olsthoorn, Ron A M Fouchier
The influenza A virus genome consists of eight RNA segments. RNA structures within these segments and complementary (cRNA) and protein-coding mRNAs may play a role in virus replication. Here, conserved putative secondary structures that impose significant evolutionary constraints on the gene segment encoding the surface glycoprotein hemagglutinin (HA) were investigated using available sequence data on tens of thousands of virus strains. Structural constraints were identified by analysis of covariations of nucleotides suggested to be paired by structure prediction algorithms...
December 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27941311/%C3%AE-klotho-as-a-negative-regulator-of-the-peptide-transporters-pept1-and-pept2
#6
Abeer Abousaab, Jamshed Warsi, Madhuri S Salker, Florian Lang
BACKGROUND/AIMS: β-Klotho, a transmembrane protein expressed in several tissues including the brain and the kidney, is critically important for inhibition of 1,25(OH)2D3 formation by FGF23. The extracellular domain of Klotho protein could be cleaved off, thus being released into blood or cerebrospinal fluid. Soluble klotho is a β-glucuronidase participating in the regulation of several ion channels and carriers. The present study explored the effect of β-Klotho protein on the peptide transporters PEPT1 and PEPT2...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27935778/anesthesia-involvement-in-palliative-care
#7
Amanda C Faircloth
Palliative care teams require multidisciplinary support. While this is an emerging area in anesthesia practice, there are many avenues for certified registered nurse anesthetists (CRNAs) to share their unrivaled clinical knowledge. CRNAs may become involved with or consult on palliative sedation, medical management, interventional pain management, terminal wean/extubation, and organ donation. Additionally, CRNAs need to understand the unique needs of this patient population so that they can appropriately care for palliative care patients presenting to the operating room for palliative surgery...
January 2017: Annual Review of Nursing Research
https://www.readbyqxmd.com/read/27918405/certified-registered-nurse-anesthetist-perceptions-of-factors-impacting-patient-safety
#8
Susan P McMullan, Charlotte Thomas-Hawkins, Maria R Shirey
Certified registered nurse anesthetists (CRNAs) provide more than 40 million anesthetics each year in the United States. This article describes a study that investigates relationships among CRNA organizational structures (CRNA practice models, work setting, workload, level of education, work experience), CRNA ratings of patient safety culture, and CRNA adverse anesthesia-related event (ARE) reporting. This is a cross-sectional survey study of 336 CRNAs randomly selected from American Association of Nurse Anesthetists database...
January 2017: Nursing Administration Quarterly
https://www.readbyqxmd.com/read/27898416/sgk3-sensitivity-of-large-conductance-ca2-activated-k-channel
#9
Musaab Ahmed, Myriam Fezai, Florian Lang
BACKGROUND/AIMS: Large conductance Ca2+-activated K+ channels (maxi K+ channels or BK channels) are rapidly activated by increase of cytosolic Ca2+ activity. The channels participate in the regulation of diverse functions including neuronal excitation and cell volume. The BK channels may be modified by kinases. Channel regulating kinases include the serum & glucocorticoid inducible kinase 3 (SGK3). The present study explored whether SGK3 modifies the activity of BK channels. METHODS: cRNA encoding the Ca2+ insensitive BK channel mutant BKM513I+Δ899-903 was injected into Xenopus laevis oocytes without or with additional injection of cRNA encoding wild-type SGK3, constitutively active S419DSGK3, or catalytically inactive K191NSGK3...
November 30, 2016: Neuro-Signals
https://www.readbyqxmd.com/read/27889648/pb2-substitutions-v598t-i-increase-the-virulence-of-h7n9-influenza-a-virus-in-mammals
#10
Meng Hu, Shuofeng Yuan, Ke Zhang, Kailash Singh, Qiang Ma, Jie Zhou, Hin Chu, Bo-Jian Zheng
PB2 is one of the subunits of the influenza A virus (IAV) polymerase complex. By bioinformatics analysis we identified PB2 substitutions at positions 389 and 598 among IAV isolates from humans, which might associate with viral pathogenicity. To evaluate the biological significance of these substitutions, PB2-K389R and -V598T/I mutant viruses of avian H7N9 IAVs were generated by reverse genetics. Compared to the wild type, the mutant viruses displayed an enhanced growth capacity in human and mammalian cells...
January 15, 2017: Virology
https://www.readbyqxmd.com/read/27886255/amino-acid-substitutions-v63i-or-a37s-i61t-v63i-v100a-in-the-pa-n-terminal-domain-increase-the-virulence-of-h7n7-influenza-a-virus
#11
Meng Hu, Hin Chu, Ke Zhang, Kailash Singh, Cun Li, Shuofeng Yuan, Billy K C Chow, Wenjun Song, Jie Zhou, Bo-Jian Zheng
The PA N-terminal domain (PA-Nter) is essential for viral transcription and replication. Here we identified PA-Nter substitutions A37S, I61T, V63I and V100A in recently emerged avian influenza A viruses (IAVs) with potential effect on virus pathogenicity and/or host adaptation. We introduced the identified PA-Nter substitutions into avian H7N7 IAV by reverse genetics. Our results showed that single substitution V63I and combined substitutions, I61T/V63I and A37S/I61T/V63I/V100A (Mfour), significantly increased virus growth capacity in mammalian cells...
November 25, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27855402/up-regulation-of-the-excitatory-amino-acid-transporters-eaat1-and-eaat2-by-mammalian-target-of-rapamycin
#12
Abeer Abousaab, Nestor Luis Uzcategui, Bhaeldin Elsir, Florian Lang
BACKGROUND: The excitatory amino-acid transporters EAAT1 and EAAT2 clear glutamate from the synaptic cleft and thus terminate neuronal excitation. The carriers are subject to regulation by various kinases. The EAAT3 isoform is regulated by mammalian target of rapamycin (mTOR). The present study thus explored whether mTOR influences transport by EAAT1 and/or EAAT2. METHODS: cRNA encoding wild type EAAT1 (SLC1A3) or EAAT2 (SLC1A2) was injected into Xenopus oocytes without or with additional injection of cRNA encoding mTOR...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27852861/pre-mrna-processing-factor-prp18-is-a-stimulatory-factor-of-influenza-virus-rna-synthesis-and-possesses-nucleoprotein-chaperone-activity
#13
M Minakuchi, K Sugiyama, Y Kato, T Naito, M Okuwaki, A Kawaguchi, K Nagata
: The genome of influenza virus (viral RNA [vRNA]) is associated with the nucleoprotein (NP) and viral RNA-dependent RNA polymerases and forms helical viral ribonucleoprotein (vRNP) complexes. The NP-vRNA complex is the biologically active template for RNA synthesis by the viral polymerase. Previously, we identified human pre-mRNA processing factor 18 (Prp18) as a stimulatory factor for viral RNA synthesis using a Saccharomyces cerevisiae replicon system and a single-gene deletion library of Saccharomyces cerevisiae (T...
February 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/27752882/gene-expression-signal-transduction-pathways-and-functional-networks-associated-with-growth-of-sporadic-vestibular-schwannomas
#14
Hjalte C R Sass, Rehannah Borup, Mikkel Alanin, Finn Cilius Nielsen, Per Cayé-Thomasen
The objective of this study was to determine global gene expression in relation to Vestibular schwannomas (VS) growth rate and to identify signal transduction pathways and functional molecular networks associated with growth. Repeated magnetic resonance imaging (MRI) prior to surgery determined tumor growth rate. Following tissue sampling during surgery, mRNA was extracted from 16 sporadic VS. Double stranded cDNA was synthesized from the mRNA and used as template for in vitro transcription reaction to synthesize biotin-labeled antisense cRNA, which was hybridized to Affymetrix HG-U133A arrays and analyzed by dChip software...
October 17, 2016: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/27702419/expression-of-the-t85a-mutant-of-zebrafish-aquaporin-3b-improves-post-thaw-survival-of-cryopreserved-early-mammalian-embryos
#15
Sylvia J Bedford-Guaus, François Chauvigné, Eva Mejía-Ramírez, Mercè Martí, Antoni Ventura-Rubio, Ángel Raya, Joan Cerdà, Anna Veiga
While vitrification has become the method of choice for preservation of human oocytes and embryos, cryopreservation of complex tissues and of large yolk-containing cells, remains largely unsuccessful. One critical step in such instances is appropriate permeation while avoiding potentially toxic concentrations of cryoprotectants. Permeation of water and small non-charged solutes, such as those used as cryoprotectants, occurs largely through membrane channel proteins termed aquaporins (AQPs). Substitution of a Thr by an Ala residue in the pore-forming motif of the zebrafish (Dario rerio) Aqp3b paralog resulted in a mutant (DrAqp3b-T85A) that when expressed in Xenopus or porcine oocytes increased their permeability to ethylene glycol at pH 7...
December 2016: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/27694620/single-molecule-fret-reveals-the-pre-initiation-and-initiation-conformations-of-influenza-virus-promoter-rna
#16
Nicole C Robb, Aartjan J W Te Velthuis, Ralph Wieneke, Robert Tampé, Thorben Cordes, Ervin Fodor, Achillefs N Kapanidis
Influenza viruses have a segmented viral RNA (vRNA) genome, which is replicated by the viral RNA-dependent RNA polymerase (RNAP). Replication initiates on the vRNA 3' terminus, producing a complementary RNA (cRNA) intermediate, which serves as a template for the synthesis of new vRNA. RNAP structures show the 3' terminus of the vRNA template in a pre-initiation state, bound on the surface of the RNAP rather than in the active site; no information is available on 3' cRNA binding. Here, we have used single-molecule Förster resonance energy transfer (smFRET) to probe the viral RNA conformations that occur during RNAP binding and initial replication...
December 1, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27681127/ubiquitination-up-regulates-influenza-virus-polymerase-function
#17
James Kirui, Arindam Mondal, Andrew Mehle
: The influenza A virus polymerase plays an essential role in the virus lifecycle, directing synthesis of viral mRNAs and genomes. It is a trimeric complex composed of subunits PA, PB1, and PB2 and associates with viral RNAs and nucleoprotein (NP) to form higher order ribonucleoprotein (RNP) complexes. The polymerase is regulated temporally over the course of infection to ensure coordinated expression of viral genes as well as replication of the viral genome. Various host factors and processes have been implicated in regulation of the IAV polymerase function, including post-translational modifications, however the mechanisms are not fully understood...
September 28, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27637823/value-based-care-and-bundled-payments-anesthesia-care-costs-for-outpatient-oncology-surgery-using-time-driven-activity-based-costing
#18
Katy E French, Alexis B Guzman, Augustin C Rubio, John C Frenzel, Thomas W Feeley
BACKGROUND: With the movement towards bundled payments, stakeholders should know the true cost of the care they deliver. Time-driven activity-based costing (TDABC) can be used to estimate costs for each episode of care. In this analysis, TDABC is used to both estimate the costs of anesthesia care and identify the primary drivers of those costs of 11 common oncologic outpatient surgical procedures. METHODS: Personnel cost were calculated by determining the hourly cost of each provider and the associated process time of the 11 surgical procedures...
September 2016: Healthcare
https://www.readbyqxmd.com/read/27616585/microarray-gene-expression-during-early-healing-of-gbr-treated-calvarial-critical-size-defects
#19
R Al-Kattan, M Retzepi, E Calciolari, N Donos
OBJECTIVES: To investigate the gene expression and molecular pathways implicated in the regulation of the osseous healing process following guided bone regeneration (GBR). MATERIAL AND METHODS: Six 6-month-old Wistar male rats were used. Standardized 5-mm critical size defects were created in the parietal bones of each animal and treated with an extracranial and intracranial ePTFE membrane, according to the GBR principle. Three animals were randomly sacrificed after 7 and 15 days of healing...
September 11, 2016: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/27587391/the-european-eel-ncc%C3%AE-gene-encodes-a-thiazide-resistant-na-cl-cotransporter
#20
Erika Moreno, Consuelo Plata, Alejandro Rodríguez-Gama, Eduardo R Argaiz, Norma Vázquez, Karla Leyva-Ríos, León Islas, Christopher Cutler, Diana Pacheco-Alvarez, Adriana Mercado, Raquel Cariño-Cortés, María Castañeda-Bueno, Gerardo Gamba
The thiazide-sensitive Na-Cl cotransporter (NCC) is the major pathway for salt reabsorption in the mammalian distal convoluted tubule. NCC plays a key role in the regulation of blood pressure. Its inhibition with thiazides constitutes the primary baseline therapy for arterial hypertension. However, the thiazide-binding site in NCC is unknown. Mammals have only one gene encoding for NCC. The eel, however, contains a duplicate gene. NCCα is an ortholog of mammalian NCC and is expressed in the kidney. NCCβ is present in the apical membrane of the rectum...
October 21, 2016: Journal of Biological Chemistry
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