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https://www.readbyqxmd.com/read/28369513/identification-and-characterization-of-aurora-kinase-b-and-c-variants-associated-with-maternal-aneuploidy
#1
Alexandra L Nguyen, Diego Marin, Anbo Zhou, Amanda S Gentilello, Evan M Smoak, Zubing Cao, Anastasia Fedick, Yujue Wang, Deanne Taylor, Richard T Scott, Jinchuan Xing, Nathan Treff, Karen Schindler
STUDY QUESTION: Are single nucleotide variants (SNVs) in Aurora kinases B and C associated with risk of aneuploid conception? SUMMARY ANSWER: Two SNVs were found in patients with extreme aneuploid concepti rates with respect to their age; one variant, AURKC p.I79V, is benign, while another, AURKB p.L39P, is a potential gain-of-function mutant with increased efficiency in promoting chromosome alignment. WHAT IS KNOWN ALREADY: Maternal age does not always predict aneuploidy risk, and rare gene variants can be drivers of disease...
March 25, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28364522/spindlin-1-is-essential-for-metaphase-ii-stage-maintenance-and-chromosomal-stability-in-porcine-oocytes
#2
Jeong-Woo Choi, Ming-Hui Zhao, Shuang Liang, Jing Guo, Zi-Li Lin, Ying-Hua Li, Yu-Jin Jo, Nam-Hyung Kim, Xiang-Shun Cui
Study question: What is the function of Spindlin 1 (Spin1) in metaphase II stage oocytes in pigs? Summary answer: Depletion of Spin1 induces spontaneous oocyte activation and overexpression of Spin1 causes multinuclear formation through induction of DNA damage in porcine oocytes. What is known already: Little is known about the function of Spin1 in oocytes and embryos. In mouse oocytes, Spin1 is specifically expressed during gametogenesis and is essential for meiotic resumption...
March 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28333931/differential-gene-expression-in-patients-with-subsyndromal-symptomatic-depression-and-major-depressive-disorder
#3
Chengqing Yang, Guoqin Hu, Zezhi Li, Qingzhong Wang, Xuemei Wang, Chengmei Yuan, Zuowei Wang, Wu Hong, Weihong Lu, Lan Cao, Jun Chen, Yong Wang, Shunying Yu, Yimin Zhou, Zhenghui Yi, Yiru Fang
BACKGROUND: Subsyndromal symptomatic depression (SSD) is a subtype of subthreshold depressive and can lead to significant psychosocial functional impairment. Although the pathogenesis of major depressive disorder (MDD) and SSD still remains poorly understood, a set of studies have found that many same genetic factors play important roles in the etiology of these two disorders. Nowadays, the differential gene expression between MDD and SSD is still unknown. In our previous study, we compared the expression profile and made the classification with the leukocytes by using whole-genome cRNA microarrays among drug-free first-episode subjects with SSD, MDD and matched healthy controls (8 subjects in each group), and finally determined 48 gene expression signatures...
2017: PloS One
https://www.readbyqxmd.com/read/28288089/complication-rates-for-fluoroscopic-guided-interlaminar-lumbar-epidural-steroid-injections-performed-by-certified-registered-nurse-anesthetists-in-diverse-practice-settings
#4
Donald E Beissel
STUDY QUESTION: What is the complication rate for Certified Registered Nurse Anesthetists (CRNAs) performing fluoroscopic guided lumbar epidural steroid injections (LESIs)? METHODS: CRNA pain practitioners responded to questionnaires regarding their education level, experience, and practice locations. Participants also collected data on number of fluoroscopic guided LESIs performed and each of 20 complications for a 6-month period, and answered a poll regarding satisfaction with the data collection process...
November 2016: Journal for Healthcare Quality: Official Publication of the National Association for Healthcare Quality
https://www.readbyqxmd.com/read/28270562/male-infertility-linked-point-mutation-reveals-a-vital-binding-role-for-the-c2-domain-of-sperm-plc%C3%AE
#5
Michail Nomikos, Panagiotis Stamatiadis, Jessica R Sanders, Konrad Beck, Brian L Calver, Luke Buntwal, Morgan Lofty, Zili Sideratou, Karl Swann, F Anthony Lai
Sperm-specific phospholipase C zeta (PLCζ) is widely considered to be the physiological stimulus that evokes intracellular calcium (Ca(2+)) oscillations that are essential for the initiation of egg activation during mammalian fertilisation. A recent genetic study reported a male infertility case that was directly associated with a point mutation in the PLCζ C2 domain, where an isoleucine residue had been substituted with a phenylalanine (I489F). Here, we have analysed the effect of this mutation on the in vivo Ca(2+) oscillation-inducing activity and the in vitro biochemical properties of human PLCζ...
March 7, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/28235172/exploring-strategies-to-increase-and-sustain-membership-in-the-american-association-of-nurse-anesthetists
#6
Cynthia Ann Farina, Wanda Wilson, Kim FitzSimmons
The total numbers of Certified Registered Nurse Anesthetists (CRNAs) in the United States and members of the American Association of Nurse Anesthetists (AANA) have risen to an all-time high. However, the percentage of CRNAs who are AANA members has been slowly declining since 2006, particularly among newly certified nurse anesthetists. To develop new strategies to increase and sustain CRNA membership, the AANA conducted a translational research project that explored evidence-based determinants of individuals' decisions to join professional associations, current membership practices and patterns, results of a recent member needs survey, and socialization of student registered nurse anesthetists (SRNAs) into the AANA...
December 2016: AANA Journal
https://www.readbyqxmd.com/read/28232820/influenza-virus-infection-induces-host-pyruvate-kinase-m-which-interacts-with-viral-rna-dependent-rna-polymerase
#7
Yukari Miyake, Kosuke Ishii, Ayae Honda
Influenza virus RNA-dependent RNA polymerase (RdRp) is a heterotrimer of three viral proteins, PB1, PB2, and PA and is involved in both transcription and replication of the negative strand of the viral RNA (vRNA) genome. RdRp is multifunctional, possessing RNA polymerase, cap binding, and endonuclease activities. The enzyme synthesizes three different RNAs, complementary RNA (cRNA) and messenger RNA (mRNA) from vRNA, and vRNA from cRNA. To synthesize these three RNAs, RdRp requires conversion of its function by host factor...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28209435/contribution-of-equilibrative-nucleoside-transporter-ent-2-to-fluorouracil-transport-in-rat-placental-trophoblast-cells
#8
Akinori Takagi, Tomohiro Nishimura, Tomoya Akashi, Masatoshi Tomi, Emi Nakashima
Fluorouracil is used for treatment of breast cancer even in pregnant women, except during fetal organogenesis. The purpose of this study was to clarify the transport mechanism of fluorouracil at the rat placental barrier. Maternal-to-fetal transfer of [(3)H]fluorouracil in rats at gestational day 19.5 was saturable and much higher than that of [(14)C]sucrose. The uptake of [(3)H]fluorouracil was also saturable in rat placental trophoblast TR-TBT 18d-1 cells, which express both equilibrative nucleoside transporter (ENT) 1 and ENT2...
December 8, 2016: Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/28143838/cost-related-non-adherence-to-prescribed-medicines-among-older-adults-a-cross-sectional-analysis-of-a-survey-in-11-developed-countries
#9
Steven G Morgan, Augustine Lee
OBJECTIVES: To assess the effects of costs on access to medicines in 11 developed countries offering different levels of prescription drug coverage for their populations. DESIGN: Cross-sectional study of data from the Commonwealth Fund 2014 International Health Policy Survey of Older Adults. SETTING: Telephone survey conducted in 11 high-income countries: Australia, Canada, France, Germany, the Netherlands, New Zealand, Norway, Sweden, Switzerland, the UK and the USA...
January 31, 2017: BMJ Open
https://www.readbyqxmd.com/read/28126917/an-in-vitro-fluorescence-based-study-of-initiation-of-rna-synthesis-by-influenza-b-polymerase
#10
Stefan Reich, Delphine Guilligay, Stephen Cusack
Influenza polymerase replicates, via a complementary RNA intermediate (cRNA), and transcribes the eight viral RNA (vRNA) genome segments. To initiate RNA synthesis it is bound to the conserved 5΄ and 3΄ extremities of the vRNA or cRNA (the 'promoter'). 5΄-3΄ base-pairing in the distal promoter region is essential to position the template RNA at the polymerase active site, as shown by a new crystal structure with the 3΄ end threading through the template entry tunnel. We develop fluorescence polarization assays to quantify initiation of cap-primed (transcription) or unprimed (replication) RNA synthesis by recombinant influenza B polymerase bound to the vRNA or cRNA promoter...
April 7, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28122973/role-of-the-pb2-627-domain-in-influenza-a-virus-polymerase-function
#11
Benjamin E Nilsson, Aartjan J W Te Velthuis, Ervin Fodor
The RNA genome of influenza A viruses is transcribed and replicated by the viral RNA-dependent RNA polymerase, composed of the subunits PA, PB1, and PB2. High-resolution structural data revealed that the polymerase assembles into a central polymerase core and several auxiliary highly flexible, protruding domains. The auxiliary PB2 cap-binding and the PA endonuclease domains are both involved in cap snatching, but the role of the auxiliary PB2 627 domain, implicated in host range restriction of influenza A viruses, is still poorly understood...
April 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28117773/xenopus-oocytes-optimized-methods-for-microinjection-removal-of-follicular-cell-layers-and-fast-solution-changes-in-electrophysiological-experiments
#12
Maria C Maldifassi, Nisa Wongsamitkul, Roland Baur, Erwin Sigel
The Xenopus oocyte as a heterologous expression system for proteins, was first described by Gurdon et al.(1) and has been widely used since its discovery (References 2 - 3, and references therein). A characteristic that makes the oocyte attractive for foreign channel expression is the poor abundance of endogenous ion channels(4). This expression system has proven useful for the characterization of many proteins, among them ligand-gated ion channels. The expression of GABAA receptors in Xenopus oocytes and their functional characterization is described here, including the isolation of oocytes, microinjections with cRNA, the removal of follicular cell layers, and fast solution changes in electrophysiological experiments...
December 31, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28115197/structural-insights-into-rna-synthesis-by-the-influenza-virus-transcription-replication-machine
#13
REVIEW
Alexander Pflug, Maria Lukarska, Patricia Resa-Infante, Stefan Reich, Stephen Cusack
Influenza virus is a segmented, negative strand RNA virus with each genome segment being packaged in a distinct ribonucleoprotein particle (RNP). The RNP consists of the heterotrimeric viral RNA-dependent RNA polymerase bound to the conserved 5' and 3' ends of the genome segment (the viral promoter) with the rest of the viral RNA (vRNA) being covered by multiple copies of nucleoprotein. This review focusses on the new insights that recent crystal structures have given into the detailed molecular mechanisms by which the polymerase performs both transcription and replication of the vRNA genome...
January 20, 2017: Virus Research
https://www.readbyqxmd.com/read/28026817/-a-possibility-to-use-the-dna-based-probes-as-internal-standards-for-agilent-technologies-microarray-transcriptomic-analysis
#14
L K Kurbatov, V G Zgoda
Accuracy of the microarray technology results is raised by using the multi-stage normalization of results. One of the principal requirements of such normalization is usage of internal standards. The routine Agilent microarray-based gene expression analysis protocol utilizes a Spike-In Kit during preparation of the samples representing a mixture of RNA fragments in different ratios. RNA probes which were synthesized in vitro conditions could be also used to establish how the magnitude of the fluorescent signal reflects the presence of RNA in the sample...
November 2016: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
https://www.readbyqxmd.com/read/28004117/a-blood-based-gene-expression-and-signaling-pathway-analysis-to-differentiate-between-high-and-low-grade-gliomas
#15
Stephen N Ponnampalam, Nor Rizan Kamaluddin, Zubaidah Zakaria, Vickneswaran Matheneswaran, Dharmendra Ganesan, Mohammed Saffari Haspani, Mina Ryten, John A Hardy
The aims of the present study were to undertake gene expression profiling of the blood of glioma patients to determine key genetic components of signaling pathways and to develop a panel of genes that could be used as a potential blood-based biomarker to differentiate between high and low grade gliomas, non-gliomas and control samples. In this study, blood samples were obtained from glioma patients, non-glioma and control subjects. Ten samples each were obtained from patients with high and low grade tumours, respectively, ten samples from non-glioma patients and twenty samples from control subjects...
January 2017: Oncology Reports
https://www.readbyqxmd.com/read/28001018/advanced-practice-registered-nurses-final-rule-with-comment-period
#16
(no author information available yet)
The Department of Veterans Affairs (VA) is amending its medical regulations to permit full practice authority of three roles of VA advanced practice registered nurses (APRN) when they are acting within the scope of their VA employment. Certified Registered Nurse Anesthetists (CRNA) will not be included in VA's full practice authority under this final rule, but comment is requested on whether there are access issues or other unconsidered circumstances that might warrant their inclusion in a future rulemaking...
December 14, 2016: Federal Register
https://www.readbyqxmd.com/read/27978824/unspecific-binding-of-crna-probe-to-plaques-in-two-mouse-models-for-alzheimer-s-disease
#17
Anne Schaarschuch, Christoph Redies, Nicole Hertel
BACKGROUND: Alzheimer's disease (AD) is characterized by the pathological deposition of amyloid-β (Aβ) protein-containing plaques. Microglia and astrocytes are commonly attracted to the plaques by an unknown mechanism that may involve cell adhesion. One cell adhesion family of proteins, the cadherins, are widely expressed in the central nervous system. Therefore, our study was designed to map the expression of cadherins in AD mouse brains. A particular focus was on plaques because diverse mRNA-species were found in plaques and their surrounding area in brains of AD patients...
December 16, 2016: Journal of Negative Results in Biomedicine
https://www.readbyqxmd.com/read/27966593/subtype-specific-structural-constraints-in-the-evolution-of-influenza-a-virus-hemagglutinin-genes
#18
Alexander P Gultyaev, Monique I Spronken, Mathilde Richard, Eefje J A Schrauwen, René C L Olsthoorn, Ron A M Fouchier
The influenza A virus genome consists of eight RNA segments. RNA structures within these segments and complementary (cRNA) and protein-coding mRNAs may play a role in virus replication. Here, conserved putative secondary structures that impose significant evolutionary constraints on the gene segment encoding the surface glycoprotein hemagglutinin (HA) were investigated using available sequence data on tens of thousands of virus strains. Structural constraints were identified by analysis of covariations of nucleotides suggested to be paired by structure prediction algorithms...
December 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27941311/%C3%AE-klotho-as-a-negative-regulator-of-the-peptide-transporters-pept1-and-pept2
#19
Abeer Abousaab, Jamshed Warsi, Madhuri S Salker, Florian Lang
BACKGROUND/AIMS: β-Klotho, a transmembrane protein expressed in several tissues including the brain and the kidney, is critically important for inhibition of 1,25(OH)2D3 formation by FGF23. The extracellular domain of Klotho protein could be cleaved off, thus being released into blood or cerebrospinal fluid. Soluble klotho is a β-glucuronidase participating in the regulation of several ion channels and carriers. The present study explored the effect of β-Klotho protein on the peptide transporters PEPT1 and PEPT2...
2016: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/27935778/anesthesia-involvement-in-palliative-care
#20
Amanda C Faircloth
Palliative care teams require multidisciplinary support. While this is an emerging area in anesthesia practice, there are many avenues for certified registered nurse anesthetists (CRNAs) to share their unrivaled clinical knowledge. CRNAs may become involved with or consult on palliative sedation, medical management, interventional pain management, terminal wean/extubation, and organ donation. Additionally, CRNAs need to understand the unique needs of this patient population so that they can appropriately care for palliative care patients presenting to the operating room for palliative surgery...
January 2017: Annual Review of Nursing Research
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