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https://www.readbyqxmd.com/read/28647792/regeneration-of-hyaline-cartilage-promoted-by-xenogeneic-mesenchymal-stromal-cells-embedded-within-elastin-like-recombinamer-based-bioactive-hydrogels
#1
David Pescador, Arturo Ibáñez-Fonseca, Fermín Sánchez-Guijo, Jesús G Briñón, Francisco Javier Arias, Sandra Muntión, Cristina Hernández, Alessandra Girotti, Matilde Alonso, María Consuelo Del Cañizo, José Carlos Rodríguez-Cabello, Juan Francisco Blanco
Over the last decades, novel therapeutic tools for osteochondral regeneration have arisen from the combination of mesenchymal stromal cells (MSCs) and highly specialized smart biomaterials, such as hydrogel-forming elastin-like recombinamers (ELRs), which could serve as cell-carriers. Herein, we evaluate the delivery of xenogeneic human MSCs (hMSCs) within an injectable ELR-based hydrogel carrier for osteochondral regeneration in rabbits. First, a critical-size osteochondral defect was created in the femora of the animals and subsequently filled with the ELR-based hydrogel alone or with embedded hMSCs...
August 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28646121/at1-receptor-blockade-attenuates-outward-aortic-remodeling-associated-with-diet-induced-obesity-in-mice
#2
Friedrich Krueger, Kai Kappert, Anna Foryst-Ludwig, Frederike Kramer, Markus Clemenz, Aleksandra Grzesiak, Manuela Sommerfeld, Jan Paul Bernhard Frese, Andreas Greiner, Ulrich Kintscher, Thomas Unger, Elena Kaschina
The renin-angiotensin-system and obesity have been implicated in vascular outward remodeling, including aneurysms, but the precise mechanisms are yet not understood. We investigated the effect of the angiotensin AT1-receptor antagonist telmisartan on aortic outward remodeling in a diet-induced obesity-model in mice. C57/Black6J mice were fed with either a low-fat diet (LFD), or a high-fat diet (HFD) for 14 weeks. One group of HFD-mice was additionally exposed to telmisartan (3mg/kg/d) for the last 4 weeks. HFD led to aortic outward remodeling, characterized by increased proteolysis, along with structural changes, such as fragmentation of elastic fibres, and decreased elastin content...
June 23, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28642715/elastin-like-protein-with-statherin-derived-peptide-controls-fluorapatite-formation-and-morphology
#3
Kseniya Shuturminska, Nadezda V Tarakina, Helena S Azevedo, Andrew J Bushby, Alvaro Mata, Paul Anderson, Maisoon Al-Jawad
The process of enamel biomineralization is multi-step, complex and mediated by organic molecules. The lack of cells in mature enamel leaves it unable to regenerate and hence novel ways of growing enamel-like structures are currently being investigated. Recently, elastin-like protein (ELP) with the analog N-terminal sequence of statherin (STNA15-ELP) has been used to regenerate mineralized tissue. Here, the STNA15-ELP has been mineralized in constrained and unconstrained conditions in a fluoridated solution...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28641567/biomarkers-in-acute-aortic-syndromes
#4
Mustafa Yildiz, Dogac Oksen, Michael Behnes, Ibrahim Akin
BACKGROUND: Acute aortic syndromes, being mostly underdiagnosed due to unspecific symptoms, are associated with high morbidity and mortality. Diagnosis carried out by transesophageal echocardiography, computed tomography and magnetic resonance imaging. However, there are lots of biochemical assays being investigated, but none of them used reliably to identify acute aortic syndromes. Biomarkers could accelerate the diagnostic time with cost effective way and could get place in definitive diagnostic algorithm of acute aortic syndrome...
June 14, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28639381/elastin-like-polypeptide-switches-a-design-strategy-to-detect-multimeric-proteins
#5
Jugal Dhandhukia, Dab Brill, Aida Kouhi, Martha Pastuszka, John Andrew MacKay
Elastin-Like Polypeptides (ELPs) reversibly phase separate in response to changes in temperature, pressure, concentration, pH, and ionic species. While powerful triggers, biological microenvironments present a multitude of more specific biological cues, such as antibodies, cytokines, and cell-surface receptors. To develop better biosensors and bioresponsive drug carriers, rational strategies are required to sense and respond to these target proteins. We recently reported that non-covalent association of two ELP fusion proteins to a 'chemical inducer of dimerization' small molecule (1...
June 22, 2017: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/28637775/increased-proximal-aortic-diameter-is-associated-with-risk-of-cardiovascular-events-and-all-cause-mortality-in-blacks-the-jackson-heart-study
#6
Daisuke Kamimura, Takeki Suzuki, Solomon K Musani, Michael E Hall, Tandaw E Samdarshi, Adolfo Correa, Ervin R Fox
BACKGROUND: Enlargement of the proximal aorta is associated with aortic wall tissue remodeling, including fragmentation of the elastin fibers, increased synthesis of collagen, and calcification, all of which are associated with aortic wall stiffening. We hypothesized that the proximal aortic diameter (AoD) is associated with cardiovascular events in a community-based cohort of blacks. METHODS AND RESULTS: We investigated the associations between AoD and cardiovascular events among 3018 black participants (mean age, 55...
June 21, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28629091/polyurethane-silica-hybrid-foams-from-a-one-step-foaming-reaction-coupled-with-a-sol-gel-process-for-enhanced-wound-healing
#7
Eun-Ho Song, Seol-Ha Jeong, Ji-Ung Park, Sukwha Kim, Hyoun-Ee Kim, Juha Song
Polyurethane (PU)-based dressing foams have been widely used due to their excellent water absorption capability, optimal mechanical properties, and unequaled economic advantage. However, the low bioactivity and poor healing capability of PU limit the applications of PU dressings in complex wound healing cases. To resolve this problem, this study was carried out the hybridization of bioactive silica nanoparticles with PU through a one-step foaming reaction that is coupled with the sol-gel process. The hybridization with silica did not affect the intrinsically porous microstructure of PU foams with silica contents of up to 10wt% and where 5-60nm silica nanoparticles were well dispersed in the PU matrix, despite slight agglomerations...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28626595/williams-beuren-syndrome-and-congenital-lobar-emphysema-uncommon-association-with-common-pathology
#8
Timothy Andrew Walsh, Krishna Revanna Gopagondanahalli, Atul Malhotra
INTRODUCTION: Congenital lobar emphysema (CLE) and Williams-Beuren Syndrome are two rare conditions that have only been reported together in a single case study. CASE PRESENTATION: We report another case of a male Caucasian newborn with nonspecific initial respiratory distress, with detection of CLE on repeat chest X-ray on Day 25 of life and concurrent ventricular septal defect, supravalvular aortic stenosis, and branch pulmonary stenosis, in whom a 7q11.23 deletion consistent with Williams-Beuren Syndrome was made...
2017: Case Reports in Pediatrics
https://www.readbyqxmd.com/read/28624929/latex-peptidases-of-calotropis-procera-for-dehairing-of-leather-as-an-alternative-to-environmentally-toxic-sodium-sulfide-treatment
#9
Laura M I Lopéz, Carolina A Viana, María E Errasti, María L Garro, José E Martegani, Germán A Mazzilli, Cléverson D T Freitas, Ídila M S Araújo, Rafaela O da Silva, Márcio V Ramos
Dehairing of crude leather is a critical stage performed at the beginning of its processing to obtain industrially useful pieces. Tanneries traditionally apply a chemical process based on sodium sulfide. Since this chemical reactive is environmentally toxic and inefficiently recycled, innovative protocols for reducing or eliminating its use in leather depilation are welcomed. Therefore, latex peptidases from Calotropis procera (CpLP) and Cryptostegia grandiflora (CgLP) were assayed for this purpose. Enzyme activity on substrates representative of skin such as hide powder azure (UHPA), elastin (UE), azocollagen (UAZOCOL), keratin (UK), and epidermis (UEP) was determined, while depilation activity was assayed on cow hide...
June 17, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28624377/evaluation-of-a-biomimetic-3d-substrate-based-on-the-human-elastin-like-polypeptides-helps-model-system-for-elastolytic-activity-detection
#10
Lucia Corich, Marina Busetti, Vincenzo Petix, Sabina Passamonti, Antonella Bandiera
Elastin is a fibrous protein that confers elasticity to tissues such as skin, arteries and lung. It is extensively cross-linked, highly hydrophobic and insoluble. Nevertheless, elastin can be hydrolysed by bacterial proteases in infectious diseases, resulting in more or less severe tissue damage. Thus, development of substrates able to reliably and specifically detect pathogen-secreted elastolytic activity is needed to improve the in vitro evaluation of the injury that bacterial proteases may provoke. In this work, two human biomimetic elastin polypeptides, HELP and HELP1, as well as the matrices derived from HELP, have been probed as substrates for elastolytic activity detection...
June 14, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28624291/vascular-lysyl-oxidase-over-expression-alters-extracellular-matrix-structure-and-induces-oxidative-stress
#11
Saray Varona, Ana B García-Redondo, Jose Martínez-González, Mercedes Salaices, Ana M Briones, Cristina Rodríguez
INTRODUCTION: Lysyl oxidase (LOX) participates in the assembly of collagen and elastin fibres. The impact of vascular LOX over-expression on extracellular matrix (ECM) structure and its contribution to oxidative stress has been analysed. METHODS: Studies were conducted on mice over-expressing LOX (Tg), specifically in smooth muscle cells (VSMC). Gene expression was assessed by real-time PCR analysis. Sirius Red staining, H2O2 production and NADPH oxidase activity were analysed in different vascular beds...
June 14, 2017: Clínica e Investigación en Arteriosclerosis
https://www.readbyqxmd.com/read/28624131/studying-arterial-stiffness-using-high-frequency-ultrasound-in-mice-with-alzheimer-disease
#12
Chin-Chia Huang, Hsiang-Fan Cheng, Ben-Peng Zhu, Pei-Yu Chen, Suet Theng Beh, Yu-Min Kuo, Chih-Chung Huang
Alzheimer disease (AD) is an irreversible, progressive brain disorder that causes slow loss of memory and thinking skills, normally leading to death in 3-9 y. The etiology of AD is not fully understood but is widely believed to be induced by the production and deposition of amyloid-β peptide in the brain. Recently, a correlation was discovered between amyloid-β deposition and atherosclerosis in the cerebral arteries of postmortem brains, indicating that amyloid-β promotes atherogenesis and that in turn atherosclerosis promotes brain amyloid-β accumulation...
June 14, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28619995/codependence-of-bone-morphogenetic-protein-receptor-2-and-transforming-growth-factor-%C3%AE-in-elastic-fiber-assembly-and-its-perturbation-in-pulmonary-arterial-hypertension
#13
Nancy F Tojais, Aiqin Cao, Ying-Ju Lai, Lingli Wang, Pin-I Chen, Miguel A Alejandre Alcazar, Vinicio de Jesus Perez, Rachel K Hopper, Christopher J Rhodes, Matthew A Bill, Lynn Y Sakai, Marlene Rabinovitch
OBJECTIVE: We determined in patients with pulmonary arterial (PA) hypertension (PAH) whether in addition to increased production of elastase by PA smooth muscle cells previously reported, PA elastic fibers are susceptible to degradation because of their abnormal assembly. APPROACH AND RESULTS: Fibrillin-1 and elastin are the major components of elastic fibers, and fibrillin-1 binds bone morphogenetic proteins (BMPs) and the large latent complex of transforming growth factor-β1 (TGFβ1)...
June 15, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28608428/fusion-of-plectasin-derivative-nz2114-with-hydrophilic-random-coil-polypeptide-recombinant-production-in-pichia-pastoris-and-antimicrobial-activity-against-clinical-strain-mrsa
#14
L A Lima, R de Vries, L S Biswaro, I M Vasconcelos, O L Franco, S C Dias
One of the roadblocks towards the practical use of antimicrobial peptides for medical use is their relatively high cost when synthesized chemically. Effective recombinant production has only been successful in some cases, such as the previously reported production in Pichia pastoris of the antimicrobial plectasin derivative peptide NZ2114. The same production host has also been used extensively to produce so-called protein-polymers: sequences that consist of repetitions of simple amino acid motifs found in structural proteins such as collagen and elastin, and that can be designed to self-assemble in micelles, fibers and hydrogels...
June 12, 2017: Biopolymers
https://www.readbyqxmd.com/read/28608217/multiplex-cathepsin-zymography-to-detect-amounts-of-active-cathepsins-k-l-s-and-v
#15
Manu O Platt
Cysteine cathepsins are powerful proteases that can degrade other proteins, among which are the extracellular matrix proteins collagen and elastin. Multiplex cathepsin zymography is an assay that can quantify the amount of active cathepsins in a cell or tissue preparation. This method works for measuring the amounts of active cathepsins K, L, S, and V in a cell or tissue preparation without requiring the use of antibodies for specific identification which tremendously reduces cost. This chapter will demonstrate the utility and interpretation of this method with mammalian cells and tissue to quantify amounts of active cathepsins K, L, S, and V without complicating signals of the procathepsin...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28608200/mmp-activity-detection-in-zymograms
#16
Péter Bencsik, Monika Bartekova, Anikó Görbe, Krisztina Kiss, János Pálóczi, Jana Radosinska, Gergő Szűcs, Péter Ferdinandy
Matrix metalloproteinases (MMP) belong to a distinguished class of zinc-dependent endopeptidases. Zymography is a semi-quantitative tool for determining the activity of different MMP isoenzymes in a variety of biological samples. In substrate gel zymography, protein samples of different origin (tissue, cell lysates, plasma/serum, perfusates, other liquids) are separated in sodium dodecyl sulfate (SDS) polyacrylamide gels containing copolymerized substrate (gelatin, casein, elastin, etc.), and after incubation-enabling substrate cleavage by MMPs, MMP activities are detected after the gel staining as transparent bands against a dark-blue background...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28602894/remnant-epitope-autoimmunity-in-human-abdominal-aortic-aneurysm-a-pilot-study-with-elastin-peptides
#17
Jelle Verhoeven, Alix Lambrecht, Peter Verbrugghe, Paul Herijgers, Inge Fourneau
INTRODUCTION: Abdominal aortic aneurysm (AAA) is a prevalent disease affecting around 5% of the population over 65 years of age. The exact etiology and physiopathology of AAA still raises questions and elective surgery is currently the only treatment option for this often progressive disease. In this study we hypothesized and tested a pathophysiological model that depicts AAA as an inflammation triggered autoimmune disease with remnant vessel wall peptide fragments as the antigen. MATERIAL AND METHODS: A pilot study with male AAA patients (n=14) and male controls (n=8) was conducted...
June 7, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28591514/elastin-like-polypeptide-linkers-for-single-molecule-force-spectroscopy
#18
Wolfgang Ott, Markus A Jobst, Magnus S Bauer, Ellis Durner, Lukas F Milles, Michael A Nash, Hermann E Gaub
Single-molecule force spectroscopy (SMFS) is by now well established as a standard technique in biophysics and mechanobiology. In recent years, the technique has benefitted greatly from new approaches to bioconjugation of proteins to surfaces. Indeed, optimized immobilization strategies for biomolecules and refined purification schemes are being steadily adapted and improved, which in turn has enhanced data quality. In many previously reported SMFS studies, poly(ethylene glycol) (PEG) was used to anchor molecules of interest to surfaces and/or cantilever tips...
June 12, 2017: ACS Nano
https://www.readbyqxmd.com/read/28587197/resveratrol-4-acetoxy-resveratrol-r-equol-racemic-equol-or-s-equol-as-cosmeceuticals-to-improve-dermal-health
#19
REVIEW
Edwin D Lephart
Phytochemicals are botanical compounds used in dermatology applications as cosmeceuticals to improve skin health. Resveratrol and equol are two of the best-known polyphenolic or phytoestrogens having similar chemical structures and some overlapping biological functions to 17β-estradiol. Human skin gene expression was reviewed for 28 different biomarkers when resveratrol, 4' acetoxy resveratrol (4AR), R-equol, racemic equol or S-equol were tested. Sirtuin 1 activator (SIRT 1) was stimulated by resveratrol and 4AR only...
June 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28584589/computerized-tomography-use-in-williams-beuren-syndrome-aortopathy
#20
Neale Nicola Kalis, Leena Khalifa Sulaibikh, Saud Rashid Al Amer, Haya Yousif Al Amer
Williams-Beuren syndrome is a multisystem genetic disorder caused by hemizygous deletion on chromosome 7q11.23, encompassing about 28 genes including the elastin gene, ELN. Cardiovascular abnormalities are frequent and are related to elastin insufficiency. These abnormalities include supravalvular aortic stenosis (SVAS) in 70% of case, pulmonic valve stenosis, and renal artery stenosis. Definitive therapy for supravalvar aortic stenosis consists of surgical correction of the arteriopathies. Outcomes after surgical correction of SVAS depend on the extent of the arteriopathy and the presence of other associated lesions...
January 2017: Heart Views: the Official Journal of the Gulf Heart Association
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