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https://www.readbyqxmd.com/read/28822574/analysis-of-individual-and-total-microcystins-in-surface-water-by-on-line-preconcentration-and-desalting-coupled-to-liquid-chromatography-tandem-mass-spectrometry
#1
Gabriel Munoz, Sung Vo Duy, Audrey Roy-Lachapelle, Barry Husk, Sébastien Sauvé
A fast and high-throughput method is proposed for the determination of total microcystins (ΣMC) in environmental surface waters. After a 1-h Lemieux-von Rudloff oxidation step to yield the 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) moiety, samples were quenched, filtered, and directly analyzed. This was achieved via solid phase extraction (SPE) coupled on-line to ultra-high performance liquid chromatography electrospray ionization triple stage quadrupole mass spectrometry. The choice of on-line SPE settings was conducted using experimental designs...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28822035/identification-and-functional-characterization-of-a-p-coumaroyl-coa-2-hydroxylase-involved-in-the-biosynthesis-of-coumarin-skeleton-from-peucedanum-praeruptorum-dunn
#2
Ruolan Yao, Yucheng Zhao, Tingting Liu, Chuanlong Huang, Sheng Xu, Ziwei Sui, Jun Luo, Lingyi Kong
A p-coumaroyl CoA 2'-hydroxylase responsible for the formation of coumarin lactone ring was identified from Peucedanum praeruptorum Dunn and functionally characterized in vitro. Coumarins are important plant secondary metabolites with a variety of biological activities. Ortho-hydroxylation of cinnamates leads to the formation of coumarin lactone ring and is generally thought to be a key step in coumarin biosynthesis. However, ortho-hydroxylases, especially p-coumaroyl CoA 2'-hydroxylase (C2'H) responsible for the biosynthesis of the most common coumarin skeleton, have received insufficient attention...
August 18, 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/28821843/broadband-polarization-conversion-with-anisotropic-plasmonic-metasurfaces
#3
Wei Cao, Xiaodong Yang, Jie Gao
Metasurfaces offer exciting opportunities that enable precise control of light propagation, optical intensity, phase and polarization. Plasmonic metasurface based quarter-wave plates have been recently studied to realize the conversion between linear polarization and circular polarization. However, it is still quite challenging to directly measure the birefringent phase retardation introduced by metasurface wave plates with a reliable technique. Here, we report a high-performance broadband metasurface quarter-wave plate made of anisotropic T-shaped plasmonic antennas in near-infrared wavelength range, where the achromatic nearly 90° transmitted phase retardation through the metasurface is precisely characterized with an optical vortex based interferometric approach...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821169/predictors-of-pre-procedural-concentrations-of-direct-oral-anticoagulants-a-prospective-multicentre-study
#4
Anne Godier, Anne-Sophie Dincq, Anne-Céline Martin, Adrian Radu, Isabelle Leblanc, Marion Antona, Marc Vasse, Jean-Louis Golmard, François Mullier, Isabelle Gouin-Thibault
Aims: Patients receiving direct oral anticoagulants (DOACs) frequently undergo elective invasive procedures. Their management is challenging. We aimed to determine the optimal duration of DOAC discontinuation that ensures a minimal anticoagulant effect during the procedure. Methods and results: This prospective multicentre study included 422 DOAC-treated patients requiring an invasive procedure. Pre-procedural DOAC concentration ([DOAC]) and routine haemostasis assays were performed to determine i/the proportion of patients who achieved a minimal pre-procedural [DOAC] (≤30 ng/mL) according to the duration of DOAC discontinuation, ii/the predictors of minimal [DOAC] and, iii/the ability of routine assays to predict minimal [DOAC]...
August 14, 2017: European Heart Journal
https://www.readbyqxmd.com/read/28820963/ketone-body-acetoacetate-buffers-methylglyoxal-via-a-non-enzymatic-conversion-during-diabetic-and-dietary-ketosis
#5
Trine Salomón, Christian Sibbersen, Jakob Hansen, Dieter Britz, Mads Vandsted Svart, Thomas Schmidt Voss, Niels Møller, Niels Gregersen, Karl Anker Jørgensen, Johan Palmfeldt, Thomas Bjørnskov Poulsen, Mogens Johannsen
The α-oxoaldehyde methylglyoxal is a ubiquitous and highly reactive metabolite known to be involved in aging- and diabetes-related diseases. If not detoxified by the endogenous glyoxalase system, it exerts its detrimental effects primarily by reacting with biopolymers such as DNA and proteins. We now demonstrate that during ketosis, another metabolic route is operative via direct non-enzymatic aldol reaction between methylglyoxal and the ketone body acetoacetate, leading to 3-hydroxyhexane-2,5-dione. This novel metabolite is present at a concentration of 10%-20% of the methylglyoxal level in the blood of insulin-starved patients...
August 17, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28820858/technical-and-tactical-aspects-in-italian-youth-rugby-union-in-relation-to-different-academies-regional-tournaments-and-outcomes
#6
Alexandru Nicolae Ungureanu, Giancarlo Condello, Simonluca Pistore, Daniele Conte, Corrado Lupo
This study aimed to analyze the technical and tactical aspects of the Italian under-18 Academy Rugby Union in relation to different academies, regional tournaments, and game outcomes. A notational analysis (forty-four indicators) was performed on 16 games (2014-15 season) to evaluate strong differences (P ≤ 0.05; moderate-large effect sizes) according to variables. Among academies, strong differences were showed for defensive breakdown where the defending support is much (range=77-87%), equal (range=11-32%), and less (range=2-12%) numerous than the attacking support, total tackles (range=64-122) and passes (range=72-151), pass to possession ratio (range=6-10), possession lost due to an error (range=28-59%), and ball in play in own (range=8-25%) and opponent (range=7-31%) 22m area indicators...
August 15, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28820431/high-throughput-screening-assay-for-laccase-engineering-toward-lignosulfonate-valorization
#7
David Rodríguez-Escribano, Felipe de Salas, Isabel Pardo, Susana Camarero
Lignin valorization is a pending issue for the integrated conversion of lignocellulose in consumer goods. Lignosulfonates (LS) are the main technical lignins commercialized today. However, their molecular weight should be enlarged to meet application requirements as additives or dispersing agents. Oxidation of lignosulfonates with fungal oxidoreductases, such as laccases, can increase the molecular weight of lignosulfonates by the cross-linking of lignin phenols. To advance in this direction, we describe here the development of a high-throughput screening (HTS) assay for the directed evolution of laccases, with lignosulfonate as substrate and the Folin-Ciocalteau reagent (FCR), to detect the decrease in phenolic content produced upon polymerization of lignosulfonate by the enzyme...
August 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28820195/photoelectron-spectroscopy-of-isolated-luciferin-and-infraluciferin-anions-in-vacuo-competing-photodetachment-photofragmentation-and-internal-conversion
#8
Joanne L Woodhouse, Mariana Assmann, Michael A Parkes, Helen Grounds, Steven J Pacman, James C Anderson, Graham A Worth, Helen H Fielding
The electronic structure and excited-state dynamics of the ubiquitous bioluminescent probe luciferin and its furthest red-shifted analogue infraluciferin have been investigated using photoelectron spectroscopy and quantum chemistry calculations. In our electrospray ionization source, the deprotonated anions are formed predominantly in their phenolate forms and are directly relevant to studies of luciferin and infraluciferin as models for their unstable oxyluciferin and oxyinfraluciferin emitters. Following photoexcitation in the range 357-230 nm, we find that internal conversion from high-lying excited states to the S1(1ππ*) state competes efficiently with electron detachment...
August 18, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28819757/development-of-cyp21a2-genotyping-assay-for-the-diagnosis-of-congenital-adrenal-hyperplasia
#9
Mayara Jorgens Prado, Simone Martins de Castro, Cristiane Kopacek, Maricilda Palandi de Mello, Thaiane Rispoli, Tarciana Grandi, Cláudia Maria Dornelles da Silva, Maria Lucia Rosa Rossetti
BACKGROUND: Steroid 21-hydroxylase deficiency due to CYP21A2 gene mutations represents more than 90% of all congenital adrenal hyperplasia cases. This deficiency is screened by measuring levels of 17-hydroxyprogesterone, which may vary, causing false positive or false negative results. In order to assist the diagnosis, molecular methodologies have been employed. This work aimed to perform genotyping assays to detect mutations in the CYP21A2 gene and compare the findings with other population studies...
August 17, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28819696/what-are-the-treatment-options-for-blister-like-aneurysms
#10
REVIEW
Torstein R Meling
Treatment of blood blister-like aneurysms (BBAs) of the supraclinoidal internal carotid artery (ICA) is challenging and the optimal treatment is yet to be defined. The treatment options for ICA BBAS are microsurgery, endovascular therapy, or a combination of the two. The microsurgical armamentarium consists mainly of direct aneurysm clipping with or without protective EC-IC bypass, clip-wrapping, or trap ligation with ICA sacrifice with or without EC-IC bypass. The endovascular treatment options are mainly endovascular ICA ligation, multiple overlapping stents (≥ 3) with or without coiling, covered stents, or flow diverters...
August 17, 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/28819287/characterization-of-polycrystalline-lead-oxide-for-application-in-direct-conversion-x-ray-detectors
#11
O Semeniuk, O Grynko, G Decrescenzo, G Juska, K Wang, A Reznik
While polycrystalline lead oxide (poly-PbO) is known to be one of the most promising photoconductors for utilization in X-ray detectors, its major performance parameters such as charge yield and mobility-lifetime product (μτ) are still not well established and require further investigation. Combining the conventional X-ray induced photocurrent and pulse height spectroscopy techniques we examine the X-ray photogeneration and recombination processes in poly-PbO. The measurements indicate that the amount of energy required to release a single electron hole pair W ± (inverse of charge yield) strongly depends on applied electric field and at 10 V/μm reaches ~20 eV/ehp...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818798/advances-towards-understanding-and-engineering-direct-interspecies-electron-transfer-in-anaerobic-digestion
#12
REVIEW
Sajib Barua, Bipro Ranjan Dhar
Direct interspecies electron transfer (DIET) is a recently discovered microbial syntrophy where cell-to-cell electron transfer occurs between syntrophic microbial species. DIET between bacteria and methanogenic archaea in anaerobic digestion can accelerate the syntrophic conversion of various reduced organic compounds to methane. DIET-based syntrophy can naturally occur in some anaerobic digester via conductive pili, however, can be engineered via the addition of various non-biological conductive materials...
August 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28818623/leptospira-species-molecular-epidemiology-in-the-genomic-era
#13
K Caimi, S Repetto, V Varni, P Ruybal
Leptospirosis is a zoonotic disease which global burden is increasing often related to climatic change. Hundreds of whole genome sequences from worldwide isolates of Leptospira spp. are available nowadays, together with online tools that permit to assign MLST sequence types (STs) directly from raw sequence data. In this work we have applied R7L-MLST to near 500 genomes and strains collection globally distributed. All 10 pathogenic species as well as intermediate were typed using this MLST scheme. The correlation observed between STs and serogroups in our previous work, is still satisfied with this higher dataset sustaining the implementation of MLST to assist serological classification as a complementary approach...
August 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28818321/influence-of-computed-tomography-contrast-agent-on-radiotherapy-dose-calculation-for-pancreatic-carcinoma-a-dosimetric-study-based-on-tomotherapy-and-volumetric-modulated-arc-therapy-techniques
#14
Feng Zhu, Weizhang Wu, Fuhai Zhu, Yong Wang, Yingjie Wang, Tingyi Xia
The main purpose of our investigation was to quantify the dosimetric influence of intravenous contrast agent for pancreatic cancer radiotherapy treatment. This study focused on complex modulated irradiation techniques of tomotherapy (TOMO) and volumetric-modulated arc therapy (VMAT) to investigate if novel conformal treatment methods could reduce the influence of contrast agent. In our study, patients with pancreatic cancer were enrolled to have 2 computed tomography (CT) scans in the same position without and with intravenous contrast agent for treatment planning...
August 14, 2017: Medical Dosimetry: Official Journal of the American Association of Medical Dosimetrists
https://www.readbyqxmd.com/read/28817722/nicotinamide-alone-accelerates-the-conversion-of-mouse-embryonic-stem-cells-into-mature-neuronal-populations
#15
Síle M Griffin, Mark R Pickard, Rowan P Orme, Clive P Hawkins, Adrian C Williams, Rosemary A Fricker
INTRODUCTION: Vitamin B3 has been shown to play an important role during embryogenesis. Specifically, there is growing evidence that nicotinamide, the biologically active form of vitamin B3, plays a critical role as a morphogen in the differentiation of stem cells to mature cell phenotypes, including those of the central nervous system (CNS). Detailed knowledge of the action of small molecules during neuronal differentiation is not only critical for uncovering mechanisms underlying lineage-specification, but also to establish more effective differentiation protocols to obtain clinically relevant cells for regenerative therapies for neurodegenerative conditions such as Huntington's disease (HD)...
2017: PloS One
https://www.readbyqxmd.com/read/28817290/air-stable-porous-fe2n-encapsulated-in-carbon-microboxes-with-high-volumetric-lithium-storage-capacity-and-long-cycle-life
#16
Yifan Dong, Bingliang Wang, Kangning Zhao, Yanhao Yu, Xudong Wang, Liqiang Mai, Song Jin
The development of inexpensive electrode materials with high volumetric capacity and long cycle-life is a central issue for large-scale lithium-ion batteries. Here, we report a nanostructured porous Fe2N anode fully encapsulated in carbon microboxes (Fe2N@C) prepared through a facile confined anion conversion method. The resulting carbon microboxes could not only protect the air-sensitive Fe2N from oxidation, but also retain thin and stable SEI layer. The appropriate internal voids in the Fe2N cubes help to release the volume expansion during lithiation/delithiation processes and Fe2N is kept inside the carbon microboxes without breaking the shell, resulting in a very low electrode volume expansion (the electrode thickness variation upon lithiation is ~9%)...
August 17, 2017: Nano Letters
https://www.readbyqxmd.com/read/28816605/end-of-life-in-disney-and-pixar-films-an-opportunity-for-engaging-in-difficult-conversation
#17
Kelly E Tenzek, Bonnie M Nickels
This study expanded upon previous scholarship by examining end-of-life (EOL) depictions and messages of death within Disney and Pixar animated films. We argue Disney and Pixar depictions of EOL and death can provide critical opportunities for discussing death and dying processes with children and adults alike. A content analysis of 57 movies resulted in a total of 71 character deaths. These instances of death became the discourse used for analysis. The EOL discourse was coded based on five categories (character status, depiction of death, death status, emotional reaction, and causality)...
January 1, 2017: Omega
https://www.readbyqxmd.com/read/28815764/aligning-digital-cd8-scoring-and-targeted-next-generation-sequencing-with-pd-l1-expression-a-pragmatic-approach-in-early-stage-squamous-cell-lung-carcinoma
#18
Esther Conde, Alejandra Caminoa, Carolina Dominguez, Antonio Calles, Stefan Walter, Barbara Angulo, Elena Sánchez, Marta Alonso, Luis Jimenez, Luis Madrigal, Florentino Hernando, Julian Sanz-Ortega, Beatriz Jimenez, Pilar Garrido, Luis Paz-Ares, Javier de Castro, Susana Hernandez, Fernando Lopez-Rios
AIMS: To study PD-L1 expression, tumour-infiltrating T lymphocytes (TILs) and the molecular context in patients with early-stage squamous cell lung carcinomas (SCCs). METHODS AND RESULTS: The study included samples from 40 patients (discovery cohort) and 29 patients (validation cohort) diagnosed with early-stage SCC. PD-L1 immunohistochemistry (IHC) was performed with three commercially available clones (E1L3N, SP263 and SP142). CD8(+) TILs were scored with a digital algorithm...
August 16, 2017: Histopathology
https://www.readbyqxmd.com/read/28814808/direct-observation-of-multiple-rotational-stacking-faults-coexisting-in-freestanding-bilayer-mos2
#19
Zuocheng Li, Xingxu Yan, Zhenkun Tang, Ziyang Huo, Guoliang Li, Liying Jiao, Li-Min Liu, Miao Zhang, Jun Luo, Jing Zhu
Electronic properties of two-dimensional (2D) MoS2 semiconductors can be modulated by introducing specific defects. One important type of defect in 2D layered materials is known as rotational stacking fault (RSF), but the coexistence of multiple RSFs with different rotational angles was not directly observed in freestanding 2D MoS2 before. In this report, we demonstrate the coexistence of three RSFs with three different rotational angles in a freestanding bilayer MoS2 sheet as directly observed using an aberration-corrected transmission electron microscope (TEM)...
August 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28813538/genome-wide-association-analysis-reveals-genetic-loci-and-candidate-genes-for-feeding-behavior-and-eating-efficiency-in-duroc-boars
#20
Rongrong Ding, Jianping Quan, Ming Yang, Xingwang Wang, Enqin Zheng, Huaqiang Yang, Disheng Fu, Yang Yang, Linxue Yang, Zicong Li, Dewu Liu, Gengyuan Cai, Zhenfang Wu, Jie Yang
Efficient use of feed resources is a challenge in the pork industry because the largest variability in expenditure is attributed to the cost of fodder. Efficiency of feeding is directly related to feeding behavior. In order to identify genomic regions controlling feeding behavior and eating efficiency traits, 338 Duroc boars were used in this study. The Illumina Porcine SNP60K BeadChip was used for genotyping. Data pertaining to individual daily feed intake (DFI), total daily time spent in feeder (TPD), number of daily visits to feeder (NVD), average duration of each visit (TPV), mean feed intake per visit (FPV), mean feed intake rate (FR), and feed conversion ratio (FCR) were collected for these pigs...
2017: PloS One
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