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https://www.readbyqxmd.com/read/28893179/conservation-and-diversification-of-small-rna-pathways-within-flatworms
#1
Santiago Fontenla, Gabriel Rinaldi, Pablo Smircich, Jose F Tort
BACKGROUND: Small non-coding RNAs, including miRNAs, and gene silencing mediated by RNA interference have been described in free-living and parasitic lineages of flatworms, but only few key factors of the small RNA pathways have been exhaustively investigated in a limited number of species. The availability of flatworm draft genomes and predicted proteomes allowed us to perform an extended survey of the genes involved in small non-coding RNA pathways in this phylum. RESULTS: Overall, findings show that the small non-coding RNA pathways are conserved in all the analyzed flatworm linages; however notable peculiarities were identified...
September 11, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28882798/modulatory-in-vitro-effect-of-stress-hormones-on-the-cytokine-response-of-rainbow-trout-and-gilthead-sea-bream-head-kidney-stimulated-with-vibrio-anguillarum-bacterin
#2
Ali Reza Khansari, David Parra, Felipe E Reyes-López, Lluís Tort
In fish, the stress response and their consequences in the immune system have been widely described. Recently, a differential cytokine regulation between rainbow trout (Oncorhynchus mykiss) and gilthead sea bream (Sparus aurata) was reported after treatment with stress hormones together with their receptor antagonists. Nevertheless, there is no evidence of whether antagonists for stress hormone receptors may influence the interaction between hormones and cytokines after bacterial administration. Thus, the aim of our study was to evaluate the cytokine expression in the presence of stress hormones (cortisol, ACTH, adrenaline), hormone receptor antagonists and inactivated Vibrio anguillarum bacterin in rainbow trout and gilthead sea bream head kidney primary cell culture (HKPCC)...
September 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28857959/ovarian-torsion-in-a-pediatric-patient-the-importance-of-repeat-imaging
#3
Christopher Skappak, Jackie L Thurston, April J Kam
A previously healthy 10-year-old girl presented to the emergency department with a 1-day history of abdominal pain. She was afebrile with an elevated white blood cell count and a negative pelvic ultrasound. The pain resolved while in hospital, and the patient was discharged home. The patient returned 10 days later, and the pain now migrated from the left flank to the subumbilical region. The patient now had an elevated white blood cell count and C-reactive protein. On pelvic ultrasound, the patient had a large midline mass and a normal right ovary...
July 3, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/28833629/olfactory-bulb-drives-respiration-coupled-beta-oscillations-in-the-rat-hippocampus
#4
André Lv Lockmann, Diego A Laplagne, Adriano Bl Tort
The synchronization of neuronal oscillations has been suggested as a mechanism to coordinate information flow between distant brain regions. In particular, the olfactory bulb (OB) and the hippocampus (HPC) have been shown to exhibit oscillations in the beta frequency range (10-20 Hz) that are likely to support communication between these structures. Here we further characterize features of beta oscillations in OB and HPC of rats anesthetized with urethane. We find that beta oscillations simultaneously appear in HPC and OB and phase-lock across structures...
August 22, 2017: European Journal of Neuroscience
https://www.readbyqxmd.com/read/28819301/asymmetry-of-the-temporal-code-for-space-by-hippocampal-place-cells
#5
Bryan C Souza, Adriano B L Tort
Hippocampal place cells convey spatial information through spike frequency ("rate coding") and spike timing relative to the theta phase ("temporal coding"). Whether rate and temporal coding are due to independent or related mechanisms has been the subject of wide debate. Here we show that the spike timing of place cells couples to theta phase before major increases in firing rate, anticipating the animal's entrance into the classical, rate-based place field. In contrast, spikes rapidly decouple from theta as the animal leaves the place field and firing rate decreases...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809585/patient-autonomy-and-doctor-s-concern-a-case-of-intravenous-drug-abuser
#6
Alok Atreya, Tanuj Kanchan, Rajeev K Shah
Intravenous substance abuse is often associated with physical and psychological harm, and often requires urgent medical attention. We report a case of a young male who presented with life-threatening complications of intravenous drug abuse, but refused to give details or agree to the proposed treatment, raising concerns for the team of doctors. A compos mentis patient has a right to decide what should be done to his body. Medical management or surgical interventions cannot be initiated without the patient's consent even if these are lifesaving procedures, or a procedure meant to benefit the patient...
January 1, 2017: Medico-legal Journal
https://www.readbyqxmd.com/read/28743008/wastewater-contamination-in-antarctic-melt-water-streams-evidenced-by-virological-and-organic-molecular-markers
#7
L F L Tort, K Iglesias, C Bueno, A Lizasoain, M Salvo, J Cristina, N Kandratavicius, L Pérez, R Figueira, M C Bícego, S Taniguchi, N Venturini, E Brugnoli, R Colina, M Victoria
Human activities in the Antarctica including tourism and scientific research have been raised substantially in the last century with the concomitant impact on the Antarctic ecosystems through the release of wastewater mainly from different scientific stations activities. The aim of this study was to assess the wastewater contamination of surface waters and sediments of three melt-water streams (11 sites) by leaking septic tanks located in the vicinity of the Uruguayan Scientific Station in the Fildes Peninsula, King George Island, Antarctica, during summer 2015...
December 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28726141/transoral-robotic-thyroidectomy-lessons-learned-from-an-initial-consecutive-series-of-24-patients
#8
Hoon Yub Kim, Young Jun Chai, Gianlorenzo Dionigi, Angkoon Anuwong, Jeremy D Richmon
BACKGROUND: Transoral thyroid surgery is an ideal method for minimally invasive thyroidectomy, as there is less flap dissection during the procedure and no postoperative scars. Nonetheless, technical obstacles have precluded the wide dissemination of this procedure. We present the surgical procedures and outcomes of transoral robotic thyroidectomy (TORT). METHODS: From September 2012 to June 2016, we performed TORT at Korea University Hospital. We used three intraoral ports and a single axillary port for the system's four robotic arms...
July 19, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28713701/transoral-robotic-thyroidectomy-tort-procedures-and-outcomes
#9
REVIEW
Jeremy D Richmon, Hoon Yub Kim
Remote-access approaches to the thyroid gland have seen a rapid evolution recently with the development of various techniques to minimize the cosmetic and quality-of-life impact of a visible scar. Most approaches have required significantly more tissue dissection and have been limited in their extent to treat contralateral disease. The transoral approach is the most recent iteration of "scarless" thyroidectomy and offers various advantages over other techniques including less tissue dissection, equal access to both sides of the neck, and a superb midline view of the anatomy...
June 2017: Gland Surgery
https://www.readbyqxmd.com/read/28705624/evaluation-of-three-layered-doxycycline-collagen-loaded-nanofiber-wound-dressing
#10
Serdar Tort, Füsun Acartürk, Arzu Beşikci
Nanofiber wound dressings have great potential for both acute and chronic wound healing. The aim of this study is to develop a wound dressing by the electrospinning method and to determine its in vitro characteristics. The viscosity and the surface tension values of the polymer solutions used in the electrospinning were measured and their suitability for electrospinning was evaluated. Nanofiber wound dressing consists of three layers. The first and the second layers are sodium alginate and chitosan nanofibers, respectively...
July 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28701481/place-and-grid-cells-in-a-loop-implications-for-memory-function-and-spatial-coding
#11
César Rennó-Costa, Adriano B L Tort
Place cells in the hippocampus and grid cells in the medial entorhinal cortex have different codes for space. However, how one code relates to the other is ill understood. Based on the anatomy of the entorhinal-hippocampal circuitry, we constructed a model of place and grid cells organized in a loop to investigate their mutual influence in the establishment of their codes for space. Using computer simulations, we first replicated experiments in rats that measured place and grid cell activity in different environments, and then assessed which features of the model account for different phenomena observed in neurophysiological data, such as pattern completion and pattern separation, global and rate remapping of place cells, and realignment of grid cells...
August 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28697819/functional-characterization-of-single-domain-cystatin-like-cysteine-proteinase-inhibitors-expressed-by-the-trematode-fasciola-hepatica
#12
Martín Cancela, Ileana Corvo, Edileuza DA Silva, Aline Teichmann, Leda Roche, Alvaro Díaz, José Fransisco Tort, Henrique B Ferreira, Arnaldo Zaha
Cystatins are small, phylogenetically conserved proteins that are tight-binding inhibitors of cysteine proteinases. The liver fluke Fasciola hepatica uses a diverse set of cysteine proteinases of the papain superfamily for host invasion, immune evasion and nutrition, but little is known about the regulation of these enzymes. The aim of this work is to characterize the cystatin repertoire of F. hepatica. For this purpose, we first surveyed the available sequence databases, identifying three different F. hepatica single-domain cystatins...
July 12, 2017: Parasitology
https://www.readbyqxmd.com/read/28697119/when-guild-interests-and-professional-obligations-collide
#13
Howard Minkoff, Jeffrey Ecker
Physicians who work in medical organizations are called on to fulfill two roles: guild members who work to advance physician interests (eg, lobbying for tort reform) and professional society members who work to advance patient interests (eg, developing clinical guidelines). Most often, physicians' self-interest and their interest in patient well-being align. When they do not, members of a guild or profession may justify the prioritization of self-interest with a form of motivated reasoning (a process wherein physicians weigh data differently depending on whether it supports their a priori beliefs)...
August 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28683971/tort-reform-and-the%C3%A2-cardiologist
#14
EDITORIAL
Hadley Wilson, Joseph S Wilson, Frank Ryan
No abstract text is available yet for this article.
July 11, 2017: Journal of the American College of Cardiology
https://www.readbyqxmd.com/read/28657871/trial-of-electrical-direct-current-therapy-versus-escitalopram-for-depression
#15
RANDOMIZED CONTROLLED TRIAL
Andre R Brunoni, Adriano H Moffa, Bernardo Sampaio-Junior, Lucas Borrione, Marina L Moreno, Raquel A Fernandes, Beatriz P Veronezi, Barbara S Nogueira, Luana V M Aparicio, Lais B Razza, Renan Chamorro, Luara C Tort, Renerio Fraguas, Paulo A Lotufo, Wagner F Gattaz, Felipe Fregni, Isabela M Benseñor
BACKGROUND: We compared transcranial direct-current stimulation (tDCS) with a selective serotonin-reuptake inhibitor for the treatment of depression. METHODS: In a single-center, double-blind, noninferiority trial involving adults with unipolar depression, we randomly assigned patients to receive tDCS plus oral placebo, sham tDCS plus escitalopram, or sham tDCS plus oral placebo. The tDCS was administered in 30-minute, 2-mA prefrontal stimulation sessions for 15 consecutive weekdays, followed by 7 weekly treatments...
June 29, 2017: New England Journal of Medicine
https://www.readbyqxmd.com/read/28652134/effects-of-thermal-stress-on-the-expression-of-glucocorticoid-receptor-complex-linked-genes-in-senegalese-sole-solea-senegalensis-acute-and-adaptive-stress-responses
#16
Vanessa Benítez-Dorta, María J Caballero, Mónica B Betancor, Manuel Manchado, Lluis Tort, Silvia Torrecillas, María J Zamorano, Marisol Izquierdo, Daniel Montero
The present study examined the short and mid-term effects of a rise in temperature from 18°C to 24°C on the expression of genes related to the stress response regulation in juveniles of Senegalese sole, Solea senegalensis. The animals were exposed to a temperature increase of 6°C, after 1month of acclimation at 18°C. After this process, samples of different tissues were collected from a total of 96 fish at four sampling points: 1h, 24h, 3days and 1week. The transcript levels of a set of genes involved in the stress response such as glucocorticoid receptors 1 and 2, corticotrophin-releasing factor, corticotrophin-releasing factor binding proteins, proopiomelanocortin A and B, and cellular stress defense (heat shock protein 70, 90AA and 90AB) were quantified at these sampling points...
October 1, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28647634/more-successful-results-with-less-energy-in-endovenous-laser-ablation-treatment-long-term-comparison-of-bare-tip-fiber980nm-laser-and-radial-tip-fiber1470nm-laser-application
#17
Ümit Arslan, Eyüpserhat Çalık, Mehmet Tort, Ziya Yıldız, Ali İhsan Tekin, Hüsnü Kamil Limandal, Mehmet Ali Kaygın, Özgür Dağ, Bilgehan Erkut
BACKGROUND: Varices and venous insufficiency are common and serious health problems in the general population which affect quality of life. Endothermal treatment of the great saphenous vein has become the first line of treatment for superficial venous reflux, and the endovenous laser ablation (EVLA) method has been widely accepted all over the world. In this method, ablation is provided by a laser fiber inserted into the lumen of the vein. Initially, the fibers were 810 nm, but today the fibers are usually 940, 980, or 1470 nm...
June 21, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28634082/cytokine-modulation-by-stress-hormones-and-antagonist-specific-hormonal-inhibition-in-rainbow-trout-oncorhynchus-mykiss-and-gilthead-sea-bream-sparus-aurata-head-kidney-primary-cell-culture
#18
Ali Reza Khansari, David Parra, Felipe E Reyes-López, Lluís Tort
A tight interaction between endocrine and immune systems takes place mainly due to the key role of head kidney in both hormone and cytokine secretion, particularly under stress situations in which the physiological response promotes the synthesis and release of stress hormones which may lead into immunomodulation as side effect. Although such interaction has been previously investigated, this study evaluated for the first time the effect of stress-associated hormones together with their receptor antagonists on the expression of cytokine genes in head kidney primary cell culture (HKPCC) of the freshwater rainbow trout (Oncorhynchus mykiss) and the seawater gilthead sea bream (Sparus aurata)...
June 19, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28599612/mycobacterioses-in-dogs-and-cats-from-buenos-aires-argentina
#19
Soledad Barandiaran, Marcela Martínez Vivot, Elvira Falzoni, María Jimena Marfil, Gabriela Pérez Tort, Paula Rovatti, Mónica Fernández, Ricardo Iachini, Fernanda Satek, Adriana Duchene, Martín José Zumárraga
Mycobacterioses can produce nonspecific clinical signs in dogs and cats that make diagnosis difficult. Furthermore, the full characterization of mycobacterial agents is not always possible or practical. We characterized mycobacteria detected through cytology in 12 dogs and 7 cats with generalized clinical signs from the province of Buenos Aires in Argentina. In dogs, molecular testing confirmed the presence of Mycobacterium avium subsp. hominissuis (MAH) in 8 cases and M. fortuitum in 1 case. All dogs were Miniature Schnauzers, suggesting that this breed may be more susceptible to M...
June 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28536507/dopamine-modulates-delta-gamma-phase-amplitude-coupling-in-the-prefrontal-cortex-of-behaving-rats
#20
Victoria Andino-Pavlovsky, Annie C Souza, Robson Scheffer-Teixeira, Adriano B L Tort, Roberto Etchenique, Sidarta Ribeiro
Dopamine release and phase-amplitude cross-frequency coupling (CFC) have independently been implicated in prefrontal cortex (PFC) functioning. To causally investigate whether dopamine release affects phase-amplitude comodulation between different frequencies in local field potentials (LFP) recorded from the medial PFC (mPFC) of behaving rats, we used RuBiDopa, a light-sensitive caged compound that releases the neurotransmitter dopamine when irradiated with visible light. LFP power did not change in any frequency band after the application of light-uncaged dopamine, but significantly strengthened phase-amplitude comodulation between delta and gamma oscillations...
2017: Frontiers in Neural Circuits
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