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https://www.readbyqxmd.com/read/28645101/co-localization-of-endogenous-arf6-and-its-activator-efa6d-in-the-granular-convoluted-tubule-cells-of-mouse-submandibular-glands-under-normal-conditions-and-when-stimulated-by-isoproterenol-noradrenaline-and-carbachol
#1
Apussara Tachow, Wipawee Thoungseabyoun, Laorrat Phuapittayalert, Kanoktip Petcharat, Hiroyuki Sakagami, Hisatake Kondo, Wiphawi Hipkaeo
OBJECTIVE: This study proposed to investigate the localization at light and electron microscopic levels of Arf6 and its activator EFA6D in the mouse submandibular gland (SMG) under normal conditions and when stimulated by adrenergic or cholinergic agonists. MATERIALS AND METHODS: SMGs of male adult mice were utilized for immunoblotting and immuno-light and -electron microscopic analyses. Isoproterenol and noradrenalin were used as adrenergics, while carbachol was used for the cholinergic stimulant...
June 13, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28645000/manipulating-cues-in-mind-wandering-verbal-cues-affect-the-frequency-and-the-temporal-focus-of-mind-wandering
#2
Manila Vannucci, Claudia Pelagatti, Igor Marchetti
Our understanding of mind wandering (MW) has dramatically increased over the past decade. A key challenge still facing research is the identification of the processes and events that directly cause and control its occurrence. In the present study we sought to shed light on this question, by investigating the effects of verbal cues on the frequency and temporal focus of MW. To this aim, we experimentally manipulated the presence of irrelevant verbal cues during a vigilance task, in two independent groups (Verbal-cues group vs...
June 20, 2017: Consciousness and Cognition
https://www.readbyqxmd.com/read/28644828/a-mutant-of-chlamydomonas-without-lhcsr-maintains-high-rates-of-photosynthesis-but-has-reduced-cell-division-rates-in-sinusoidal-light-conditions
#3
Michael Cantrell, Graham Peers
The LHCSR protein belongs to the light harvesting complex family of pigment-binding proteins found in oxygenic photoautotrophs. Previous studies have shown that this complex is required for the rapid induction and relaxation of excess light energy dissipation in a wide range of eukaryotic algae and moss. The ability of cells to rapidly regulate light harvesting between this dissipation state and one favoring photochemistry is believed to be important for reducing oxidative stress and maintaining high photosynthetic efficiency in a rapidly changing light environment...
2017: PloS One
https://www.readbyqxmd.com/read/28644177/a-case-of-intravesical-bacillus-calmette-guerin-related-endophthalmitis-and-retinitis-confirmed-with-retinal-biopsy
#4
Alison Huggins, Murtaza Adam, David Ehmann, Ralph C Eagle, Barry Malloy, Sunir J Garg
PURPOSE: To present a rare case of bilateral endogenous chorioretinitis and unilateral endophthalmitis due to Mycobacterium bovis in a patient who received intravesical bacillus Calmette-Guerin (BCG) treatment. METHODS: We present a case of a single male patient with bilateral endogenous chorioretinitis due to Mycobacterium bovis in a patient who received intravesical BCG, an attenuated strain of M. bovis widely used to treat superficial bladder cancer. The patient underwent intravitreal tap, vitrectomy, and chorioretinal biopsy with histologic examination...
June 20, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28643541/effects-of-ph-and-concentration-of-sodium-citrate-anticoagulant-on-platelet-aggregation-measured-by-light-transmission-aggregometry-induced-by-adenosine-diphosphate
#5
Ksenia Germanovich, Eti Alessandra Femia, Chun Yan Cheng, Natalia Dovlatova, Marco Cattaneo
The 2013 ISTH-SSC guidelines for the standardization of light transmission aggregometry (LTA) were largely based on expert consensus, as studies directly comparing LTA methodologies were lacking. We experimentally tested the cogency of ISTH-SSC recommendations pertaining to use of anticoagulant, in particular whether: (1) buffered citrate (BC) is preferable to unbuffered citrate (C); (2) the two recommended concentrations of sodium citrate (109 and 129 mM) are equivalent in terms of platelet aggregation (PA)...
June 23, 2017: Platelets
https://www.readbyqxmd.com/read/28642758/time-and-antigen-stimulation-history-influence-memory-cd8-t-cell-bystander-responses
#6
Matthew D Martin, Qiang Shan, Hai-Hui Xue, Vladimir P Badovinac
Memory CD8 T cells can be activated and induced to produce cytokines and increase stores of cytolytic proteins not only in response to cognate antigen (Ag) but also in response to inflammatory cytokines (bystander responses). Importantly, bystander memory CD8 T cell functions have been shown to be dependent upon memory CD8 T cell fitness, since exhausted CD8 T cells have diminished capacity to respond to inflammatory cues. While it is known that memory CD8 T cell functional abilities, including ability to produce cytokines in response to cognate Ag, change with time after initial Ag encounter and upon multiple Ag stimulations (e...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28642690/autonomy-in-depressive-patients-undergoing-dbs-treatment-informed-consent-freedom-of-will-and-dbs-potential-to-restore-it
#7
Timo Beeker, Thomas E Schlaepfer, Volker A Coenen
According to the World Health Organization, depression is one of the most common and most disabling psychiatric disorders, affecting at any given time approximately 325 million people worldwide. As there is strong evidence that depressive disorders are associated with a dynamic dysregulation of neural circuits involved in emotional processing, recently several attempts have been made to intervene directly in these circuits via deep brain stimulation (DBS) in patients with treatment-resistant major depressive disorder (MDD)...
2017: Frontiers in Integrative Neuroscience
https://www.readbyqxmd.com/read/28642339/establishment-of-a-novel-three-dimensional-primary-culture-model-for-hippocampal-neurogenesis
#8
Tatsuya Usui, Masashi Sakurai, Hideyoshi Kawasaki, Takashi Ohama, Hideyuki Yamawaki, Koichi Sato
New neurons are generated in the adult hippocampus throughout life and contribute to the functions of learning and memory. Nevertheless, the mechanisms by which disrupted neurogenesis regulates central nervous system (CNS) disorders are not fully understood. Here, we established a novel 3D culture system of hippocampal neurogenesis using air liquid interface (ALI) culture and Matrigel culture from mouse hippocampus tissues. After isolated mouse hippocampus tissue fragments were seeded into ALI wells and cultured in stemness-stimulated media containing Wnt, EGF, Noggin and R-spondin for 7 days, small spheres gradually appeared in the tissues...
June 2017: Physiological Reports
https://www.readbyqxmd.com/read/28642285/state-dependent-tms-reveals-representation-of-affective-body-movements-in-the-anterior-intraparietal-cortex
#9
Noemi Mazzoni, Christianne Jacobs, Paola Venuti, Juha Silvanto, Luigi Cattaneo
In humans, recognition of others' actions involves a cortical network that comprises, among other cortical regions, the posterior superior temporal sulcus (pSTS), where biological motion is coded and the anterior intraparietal suclus (aIPS), where movement information is elaborated in terms of meaningful goal-directed actions. This action observation system (AOS) is thought to encode neutral voluntary actions, and possibly some aspects of affective motor repertoire, but the role of the AOS' areas in processing affective kinematic information has never been examined...
June 22, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28641988/wavy-multistratified-amacrine-cells-in-the-monkey-retina-contain-immunoreactive-secretoneurin
#10
Andrea S Bordt, Ye Long, Nobuo Kouyama, Elizabeth S Yamada, Jens Hannibal, David W Marshak
The goals of this study were to describe the morphology, neurotransmitter content and synaptic connections of neurons in primate retinas that contain the neuropeptide secretoneurin. Amacrine cells were labeled with antibodies to secretoneurin in macaque and baboon retinas. Their processes formed three distinct plexuses in the inner plexiform layer: one in the outermost stratum, one in the center and one in the innermost stratum. In light microscopic double immunolabeling experiments, GABA was colocalized with secretoneurin in these cells, but glycine transporter 1 and Substance P were not...
June 19, 2017: Peptides
https://www.readbyqxmd.com/read/28641178/stimulatory-effect-of-aggregatibacter-actinomycetemcomitans-dna-on-proinflammatory-cytokine-expression-by-human-gingival-fibroblasts
#11
Uriel Soto-Barreras, Gabriela Cortés-Sandoval, Ruben Dominguez-Perez, Alejandra Loyola-Leyva, Panfilo-Raymundo Martinez-Rodriguez, Juan Pablo Loyola-Rodriguez
OBJECTIVE: While different virulence factors have been reported of Aggregatibacter actinomycetemcomitans (Aa), there is little information about the stimulatory effect of its DNA. The main purpose of this study was to assess the inflammatory response of human gingival fibroblasts (HGFs) stimulated with A. actinomycetemcomitans DNA. DESIGN: Cytokine levels of IL-6, IL-1α and TNF-α were measured on the supernatant of HGFs activated with 10, 25, 50 and 100μg/ml DNA of Aa during 24h...
June 15, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28640532/nonvolatile-perovskite-based-photomemory-with-a-multilevel-memory-behavior
#12
Jung-Yao Chen, Yu-Cheng Chiu, Yen-Ting Li, Chu-Chen Chueh, Wen-Chang Chen
Solution-processable organic-inorganic hybrid perovskite materials with a wealth of exotic semiconducting properties have appeared as the promising front-runners for next-generation electronic devices. Further, regarding its well photoresponsibility, various perovskite-based photosensing devices are prosperously developed in recent years. However, most exploited devices to date only transiently transduce the optical signals into electrical circuits while under illumination, which necessitates using additional converters to further store the output signals for recording the occurrence of light stimulation...
June 22, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28638915/-optogenetic-activation-of-dorsal-hippocampal-astrocytic-rac1-blocks-the-learning-of-associative-memory
#13
Xiao-Mu Guo, Zhao-Hui Liao, Ye-Zheng Tao, Fei-Fei Wang, Lan Ma
Rac1 belongs to the family of Rho GTPases, and plays important roles in the brain function. It affects the cell migration and axon guidance via regulating the cytoskeleton and cellular morphology. However, the effect of its dynamic activation in regulating physiological function remains unclear. Recently, a photoactivatable analogue of Rac1 (PA-Rac1) has been developed, allowing the activation of Rac1 by the specific wavelength of light in living cells. Thus, we constructed recombinant adeno-associated virus (AAV) of PA-Rac1 and its light-insensitive mutant PA-Rac1-C450A under the control of the mouse glial fibrillary acidic protein (mGFAP) promoter to manipulate Rac1 activity in astrocytes by optical stimulation...
June 25, 2017: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/28637840/promoting-sleep-oscillations-and-their-functional-coupling-by-transcranial-stimulation-enhances-memory-consolidation-in-mild-cognitive-impairment
#14
Julia Ladenbauer, Josef Ladenbauer, Nadine Külzow, Rebecca de Boor, Elena Avramova, Ulrike Grittner, Agnes Flöel
Alzheimer's disease (AD) not only involves loss of memory functions but also prominent deterioration of sleep physiology, already evident in the stage of mild cognitive impairment (MCI). Cortical slow oscillations (SO, 0.5-1 Hz) and thalamo-cortical spindle activity (12-15 Hz) during sleep, and their temporal coordination, are considered critical for memory formation. We investigated the potential of slow oscillatory transcranial direct current stimulation (so-tDCS), applied during a daytime nap in a sleep state-dependent manner, to modulate these activity patterns and sleep-related memory consolidation in 9 male and 7 female human patients with MCI...
June 21, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28637830/a-light-harvesting-complex-like-protein-in-maintenance-of-photosynthetic-components-in-chlamydomonas
#15
Lei Zhao, Dongmei Cheng, Xiahe Huang, Mei Chen, Luca Dall'Osto, Jiale Xing, Liyan Gao, Lingyu Li, Yale Wang, Roberto Bassi, Lianwei Peng, Yingchun Wang, Jean-David Rochaix, Fang Huang
Using a genetic approach we have identified and characterized a novel protein, named Msf1 (Maintenance Factor for PSI), which is required for the maintenance of specific components of the photosynthetic apparatus in the green alga Chlamydomonas reinhardtii. Msf1 belongs to the superfamily of light-harvesting complex (LHC) proteins with three trans-membrane domains and consensus chlorophyll binding sites. Loss of Msf1 leads to reduced accumulation of PSI and chlorophyll-binding proteins/complexes. Msf1 is a component of a thylakoid complex containing key enzymes of the tetrapyrrole biosynthetic pathway thus revealing a possible link between Msf1 and chlorophyll biosynthesis...
June 21, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28636880/behavioral-evidence-of-oxidative-stress-by-hematophagy-in-the-ocellar-system-of-rhodnius-prolixus-st%C3%A3-l-1859-red-eyed-mutants
#16
Carlos Alberto Vija Suarez, Mario Iván Ortiz, Jorge Molina
The photonegative response to light stimulation in Rhodnius prolixus (Reduviidae, Triatominae) is modulated by compound eyes and ocelli. Screening pigments in the visual system have been shown to protect the cellular structures from the oxidative stress damage produced by blood ingestion and light stimulation. Red-eyed mutants of Rhodnius prolixus lack screening pigments in their compound eyes and ocelli and are exposed to more oxidative stress. Experiments with Rhodnius prolixus and Triatoma infestans red-eyed mutants reared from the first nymphal stage show damage in the retinas of the compound eyes and a decrease in photonegative responses due to light stimulation...
June 18, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28633807/differential-induction-of-atf3-and-ho-1-in-myeloid-cells-and-keratinocytes-via-dimethylfumarate-or-cyclosporine-a
#17
Stefanie Müller, Nikola Smatlik, Marc Burian, Kamran Ghoreschi, Martin Röcken, Amir S Yazdi
BACKGROUND: Chronic inflammatory skin diseases are characterized by controlled proliferation of keratinocytes. Here, activating transcription factor 3 (ATF3) might play a fundamental role. In these inflammatory diseases, proliferation is controlled and only rarely leads to cancer development which can be supported by an inflammatory microenvironment. ATF3 is a dual function protein as it suppresses pro-inflammatory IL-6 and IL-8, but also acts as a pro-oncogenic factor by the suppression of p53...
June 12, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28632461/consuming-ecstasy-representations-of-male-and-female-orgasm-in-mainstream-pornography
#18
Léa J Séguin, Carl Rodrigue, Julie Lavigne
Social representations, which appear in a variety of media, can influence the way sexual experiences are perceived and understood. While pornography is not the only medium in which orgasm is portrayed, it is the most explicit, and it is widespread and easily accessible. As such, pornography is an ideal medium for examining representations of male and female orgasm. PornHub's 50 most viewed videos of all time were viewed and coded for the frequency of male and female orgasm, orgasm-inducing sex acts (and whether activity inducing female orgasms included some form of clitoral stimulation), and auditory (verbal, vocal) and visual (bodily) indicators of orgasm...
June 20, 2017: Journal of Sex Research
https://www.readbyqxmd.com/read/28632030/ultraviolet-b-radiation-stimulates-the-interaction-between-nuclear-factor-of-activated-t-cells-5-nfat5-and-nuclear-factor-kappa-b-nf-%C3%AE%C2%BAb-in-human-lens-epithelial-cells
#19
Inyoung Chung, Young-Sool Hah, SunMi Ju, Ji-Hye Kim, Woong-Sun Yoo, Hee-Young Cho, Ji-Myong Yoo, Seong-Wook Seo, Wan-Sung Choi, Seong-Jae Kim
PURPOSE: Nuclear factor-kappa B (NF-κB) has been proposed as a therapeutic target for the treatment of cataracts. The authors investigated the relationship between nuclear factor of activated T cells 5 (NFAT5) and NF-κB in ultraviolet B (UVB)-irradiated human lens epithelial (HLE) cells. METHODS: Human lens epithelial B-3 (HLE-B3) cells were exposed to UVB light at a dose of 10 mJ/cm(2) and then incubated for 24 h. Cell viability was assessed by using the Cell Counting Kit-8 (CCK-8) assay...
February 26, 2017: Current Eye Research
https://www.readbyqxmd.com/read/28629963/environmental-enrichment-reduces-innate-anxiety-with-no-effect-on-depression-like-behaviour-in-mice-lacking-the-serotonin-transporter
#20
Jake Rogers, Shanshan Li, Laurence Lanfumey, Anthony J Hannan, Thibault Renoir
Along with being the main target of many antidepressant medications, the serotonin transporter (5-HTT) is known to be involved in the pathophysiology of depression and anxiety disorders. In line with this, mice with varying 5-HTT genotypes are invaluable tools to study depression- and anxiety-like behaviours as well as the mechanisms mediating potential therapeutics. There is clear evidence that both genetic and environmental factors play a role in the aetiology of psychiatric disorders. In that regard, housing paradigms which seek to enhance cognitive stimulation and physical activity have been shown to exert beneficial effects in animal models of neuropsychiatric disorders...
June 16, 2017: Behavioural Brain Research
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