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https://www.readbyqxmd.com/read/29334206/decreased-expression-of-arginine-phenylalanine-amide-related-peptide-3-gene-in-dorsomedial-hypothalamic-nucleus-of-constant-light-exposure-model-of-polycystic-ovarian-syndrome
#1
Zahra Shaaban, Mohammad Reza Jafarzadeh Shirazi, Mohammad Hossein Nooranizadeh, Amin Tamadon, Farhad Rahmanifar, Somayeh Ahmadloo, Amin Ramezani, Mohammad Javad Zamiri, Iman Razeghian Jahromi, Fatemeh Sabet Sarvestani, Omid Koohi Hosseinabadi
Background: An abnormality in pulse amplitude and frequency of gonadotropin releasing hormone (GnRH) secretion is the most characteristics of polycystic ovarian syndrome (PCOS). On the other hand, arginine-phenylalanine-amide (RFamide)-related peptide-3 (RFRP3) inhibits the secretion of GnRH in mammalian hypothalamus. The current study performed in order to investigate the expression of RFRP3 mRNA in the dorsomedial hypothalamic nucleus (DMH) after the induction of PCOS in a rat model of constant light exposure, and the possible role of parity on occurrence of PCOS...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29332274/callitriche-cophocarpa-water-starwort-proteome-under-chromate-stress-evidence-for-induction-of-a-quinone-reductase
#2
Paweł Kaszycki, Aleksandra Dubicka-Lisowska, Joanna Augustynowicz, Barbara Piwowarczyk, Wojciech Wesołowski
Chromate-induced physiological stress in a water-submerged macrophyte Callitriche cophocarpa Sendtn. (water starwort) was tested at the proteomic level. The oxidative stress status of the plant treated with 1 mM Cr(VI) for 3 days revealed stimulation of peroxidases whereas catalase and superoxide dismutase activities were similar to the control levels. Employing two-dimensional electrophoresis, comparative proteomics enabled to detect five differentiating proteins subjected to identification with mass spectrometry followed by an NCBI database search...
January 13, 2018: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29332258/photodynamic-antimicrobial-chemotherapy-pact-using-toluidine-blue-inhibits-both-growth-and-biofilm-formation-by-candida-krusei
#3
Bruna Graziele Marques da Silva, Moisés Lopes Carvalho, Isabela Bueno Rosseti, Stella Zamuner, Maricilia Silva Costa
Among non-albicans Candida species, the opportunistic pathogen Candida krusei emerges because of the high mortality related to infections produced by this yeast. The Candida krusei is an opportunistic pathogen presenting an intrinsic resistance to fluconazol. In spite of the reduced number of infections produced by C. krusei, its occurrence is increasing in some groups of patients submitted to the use of fluconazol for prophylaxis. Photodynamic antimicrobial chemotherapy (PACT) is a potential antimicrobial therapy that combines visible light and a nontoxic dye, known as a photosensitizer, producing reactive oxygen species (ROS) that can kill the treated cells...
January 13, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29332121/pattern-onset-ergs-and-veps-produced-by-patterns-arising-from-light-increment-and-decrement
#4
Dennis M Fritsch, Jane C Sowden, Dorothy A Thompson
Purpose: Our aim was to elaborate how on and off signals contribute to pattern ERGs and pattern visual evoked potentials (VEPs) by using pedestal patterns arising from incremental and decremental onset stimulation. Methods: Pattern onset/offset ERGs and VEPs were produced by black and white checks of 60' side length and 88% spatial contrast appearing in a 16° field for 200 ms from white (110 cd/m2), black (7 cd/m2), and gray (48 cd/m2) backgrounds and disappeared for 1000 ms...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29330151/interleukin-22-reverses-human-islet-dysfunction-and-apoptosis-triggered-by-hyperglycemia-and-light
#5
Shadab Abadpour, Bente Halvorsen, Afaf Sahraoui, Olle Korsgren, Pål Aukrust, Hanne Scholz
Interleukin (IL)-22 has recently been suggested as an anti-inflammatory cytokine that could protect the islet cells from inflammation- and glucose-induced toxicity. We have previously shown that the tumor necrosis factor family member, LIGHT can impair human islet function at least partly via pro-apoptotic effects. Herein, we aimed to investigate the protective role of IL-22 on human islets exposed to the combination of hyperglycemia and LIGHT. First, we found up-regulation of LIGHT receptors (LTβR and HVEM) in engrafted human islets exposed to hyperglycemia (>11 mM) for 17 days post transplantation by using a double islet transplantation mouse model as well as in human islets cultured with high glucose (HG) (20mM glucose) + LIGHT in vitro and this latter effect was attenuated by IL-22...
January 12, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29329537/the-hiv-1-accessory-proteins-nef-and-vpu-downregulate-total-and-cell-surface-cd28-in-cd4-t-cells
#6
Emily N Pawlak, Brennan S Dirk, Rajesh Abraham Jacob, Aaron L Johnson, Jimmy D Dikeakos
BACKGROUND: The HIV-1 accessory proteins Nef and Vpu alter cell surface levels of multiple host proteins to modify the immune response and increase viral persistence. Nef and Vpu can downregulate cell surface levels of the co-stimulatory molecule CD28, however the mechanism of this function has not been completely elucidated. RESULTS: Here, we provide evidence that Nef and Vpu decrease cell surface and total cellular levels of CD28. Moreover, using inhibitors we implicate the cellular degradation machinery in the downregulation of CD28...
January 12, 2018: Retrovirology
https://www.readbyqxmd.com/read/29328453/aldosterone-is-involved-in-the-pathogenesis-of-obesity-related-glomerulopathy-through-activation-of-wnt-%C3%AE-catenin-signaling-in-podocytes
#7
Jia-Jia Zhu, Yi-Pu Chen, Min Yang, Bao-Li Liu, Jing Dong, Hong-Rui Dong, Hong-Liang Rui, Hong Cheng
Obesity-related glomerulopathy (ORG) is morphologically characterized by glomerulomegaly with or without observable focal segmental glomerulosclerosis under light microscope, with decreased podocyte density and number, and with increased foot‑process width observed under electron microscope. The severity of podocyte injury is correlated with the degree of proteinuria and renal dysfunction. However, the pathogenesis of ORG is not well understood. The aim of the present study was to explore the possible pathogenic role of aldosterone (ALDO) in ORG...
January 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29327383/large-scale-noniridescent-structural-color-printing-enabled-by-infiltration-driven-nonequilibrium-colloidal-assembly
#8
Ling Bai, Van Cuong Mai, Yun Lim, Shuai Hou, Helmuth Möhwald, Hongwei Duan
Structural colors originating from interaction of light with intricately arranged micro-/nanostructures have stimulated considerable interest because of their inherent photostability and energy efficiency. In particular, noniridescent structural color with wide viewing angle has been receiving increasing attention recently. However, no method is yet available for rapid and large-scale fabrication of full-spectrum structural color patterns with wide viewing angles. Here, infiltration-driven nonequilibrium assembly of colloidal particles on liquid-permeable and particle-excluding substrates is demonstrated to direct the particles to form amorphous colloidal arrays (ACAs) within milliseconds...
January 12, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29327206/brain-photobiomodulation-therapy-a-narrative-review
#9
REVIEW
Farzad Salehpour, Javad Mahmoudi, Farzin Kamari, Saeed Sadigh-Eteghad, Seyed Hossein Rasta, Michael R Hamblin
Brain photobiomodulation (PBM) therapy using red to near-infrared (NIR) light is an innovative treatment for a wide range of neurological and psychological conditions. Red/NIR light is able to stimulate complex IV of the mitochondrial respiratory chain (cytochrome c oxidase) and increase ATP synthesis. Moreover, light absorption by ion channels results in release of Ca2+ and leads to activation of transcription factors and gene expression. Brain PBM therapy enhances the metabolic capacity of neurons and stimulates anti-inflammatory, anti-apoptotic, and antioxidant responses, as well as neurogenesis and synaptogenesis...
January 11, 2018: Molecular Neurobiology
https://www.readbyqxmd.com/read/29325558/systematic-target-function-annotation-of-human-transcription-factors
#10
Yong Fuga Li, Russ B Altman
BACKGROUND: Transcription factors (TFs), the key players in transcriptional regulation, have attracted great experimental attention, yet the functions of most human TFs remain poorly understood. Recent capabilities in genome-wide protein binding profiling have stimulated systematic studies of the hierarchical organization of human gene regulatory network and DNA-binding specificity of TFs, shedding light on combinatorial gene regulation. We show here that these data also enable a systematic annotation of the biological functions and functional diversity of TFs...
January 10, 2018: BMC Biology
https://www.readbyqxmd.com/read/29325490/allocating-less-attention-to-central-vision-during-vection-is-correlated-with-less-motion-sickness
#11
Yue Wei, Jiayue Zheng, Richard H Y So
Visually induced motion sickness (VIMS) is a common discomfort response associated with vection-provoking stimuli. It is suggested that VIMS susceptibility depends on the ability to regulate visual performance during vection. To test this, in total 29 participants, with VIMS susceptibility assessed by Motion Sickness Susceptibility Questionnaire, were recruited in three successive experiments to perform sustained attention to response tests (SARTs) while watching dot pattern stimuli known to provoke roll-vection...
January 11, 2018: Ergonomics
https://www.readbyqxmd.com/read/29325101/fauna-ecological-characteristics-and-checklist-of-the-mosquitoes-in-mazandaran-province-northern-iran
#12
Seyed Hassan Nikookar, Mahmoud Fazeli-Dinan, Shahyad Azari-Hamidian, Seyed Nouraddin Mousavi Nasab, Mohsen Aarabi, Seyyed Payman Ziapour, Ahmadali Enayati, Janet Hemingway
Mosquitoes are important vectors of human and animal diseases. This study updates current knowledge on fauna, dominance, and distribution of mosquitoes in Mazandaran Province, Northern Iran, to inform disease control effort. Larval collections, using standard dippers or droppers, and adult collections, using total catches, shelter pits, CDC light traps, and human landing catches, were performed monthly in 30 villages across 16 counties, from May to December 2014. Ovitraps, baited with hay infusion as oviposition attractants or stimulants for Aedes (Stegomyia) mosquitoes, were installed in each village and inspected weekly for eggs...
January 6, 2018: Journal of Medical Entomology
https://www.readbyqxmd.com/read/29323787/inhibition-of-uvssa-ubiquitination-suppresses-transcription-coupled-nucleotide-excision-repair-deficiency-caused-by-dissociation-from-usp7
#13
Mitsuru Higa, Kiyoji Tanaka, Masafumi Saijo
Transcription-coupled nucleotide excision repair (TC-NER) is a subpathway of nucleotide excision repair that efficiently removes transcription-blocking DNA damage from the transcribed strands of active genes. UVSSA is a causative gene for UV-sensitive syndrome (UVS S), which is an autosomal recessive disorder characterized by hypersensitivity to UV light and deficiency in TC-NER. UV-stimulated scaffold protein A (UVSSA), the product of UVSSA, forms a complex with ubiquitin-specific peptidase 7 (USP7) and is stabilized by interaction with USP7...
January 11, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29322749/time-reversed-ultrasonically-encoded-optical-focusing-through-highly-scattering-ex-vivo-human-cataractous-lenses
#14
Yan Liu, Yuecheng Shen, Haowen Ruan, Frank L Brodie, Terence T W Wong, Changhuei Yang, Lihong V Wang
Normal development of the visual system in infants relies on clear images being projected onto the retina, which can be disrupted by lens opacity caused by congenital cataract. This disruption, if uncorrected in early life, results in amblyopia (permanently decreased vision even after removal of the cataract). Doctors are able to prevent amblyopia by removing the cataract during the first several weeks of life, but this surgery risks a host of complications, which can be equally visually disabling. Here, we investigated the feasibility of focusing light noninvasively through highly scattering cataractous lenses to stimulate the retina, thereby preventing amblyopia...
January 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29319733/biomechanic-and-histologic-analysis-of-fibroblastic-effects-of-tendon-to-bone-healing-by-transforming-growth-factor-%C3%AE-1-tgf-%C3%AE-1-in-rotator-cuff-tears
#15
Chong Zhang, Yu-Jie Liu
PURPOSE: To evaluate the effect of transforming growth factor β1 (TGF-β1) on tendon-to-bone reconstruction of rotator cuff tears. METHODS: Seventy-two rat supraspinatus tendons were transected and reconstructed in situ. At 8 and 16 weeks, specimens of three groups; that is control, L-dose (low dose), and H-dose (high dose) were harvested and underwent a biomechanical test to evaluate the maximum load and stiffness values. Histology sections of the tendon-to-bone interface were identified by hematoxylin-eosin or Masson trichrome stain...
December 2017: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/29316895/daily-repetitive-sensory-stimulation-of-the-paretic-hand-for-the-treatment-of-sensorimotor-deficits-in-patients-with-subacute-stroke-reset-a-randomized-sham-controlled-trial
#16
Jan C Kattenstroth, Tobias Kalisch, Matthias Sczesny-Kaiser, Wolfgang Greulich, Martin Tegenthoff, Hubert R Dinse
BACKGROUND: Repetitive sensory stimulation (RSS) adapts the timing of stimulation protocols used in cellular studies to induce synaptic plasticity. In healthy subjects, RSS leads to widespread sensorimotor cortical reorganization paralleled by improved sensorimotor behavior. Here, we investigated whether RSS reduces sensorimotor upper limb impairment in patients with subacute stroke more effectively than conventional therapy. METHODS: A single-blinded sham-controlled clinical trial assessed the effectiveness of RSS in treating sensorimotor deficits of the upper limbs...
January 9, 2018: BMC Neurology
https://www.readbyqxmd.com/read/29316607/secondary-metabolites-of-mangrove-associated-strains-of-talaromyces
#17
REVIEW
Rosario Nicoletti, Maria Michela Salvatore, Anna Andolfi
Boosted by the general aim of exploiting the biotechnological potential of the microbial component of biodiversity, research on the secondary metabolite production of endophytic fungi has remarkably increased in the past few decades. Novel compounds and bioactivities have resulted from this work, which has stimulated a more thorough consideration of various natural ecosystems as conducive contexts for the discovery of new drugs. Thriving at the frontier between land and sea, mangrove forests represent one of the most valuable areas in this respect...
January 6, 2018: Marine Drugs
https://www.readbyqxmd.com/read/29315208/exogenous-24-epibrassinolide-interacts-with-light-to-regulate-anthocyanin-and-proanthocyanidin-biosynthesis-in-cabernet-sauvignon-vitis-vinifera-l
#18
Yali Zhou, Chunlong Yuan, Shicheng Ruan, Zhenwen Zhang, Jiangfei Meng, Zhumei Xi
Anthocyanins and proanthocyanidins (PAs) are crucial factors that affect the quality of grapes and the making of wine, which were stimulated by various stimuli and environment factors (sugar, hormones, light, and temperature). The aim of the study was to investigate the influence of exogenous 24-Epibrassinolide (EBR) and light on the mechanism of anthocyanins and PAs accumulation in grape berries. Grape clusters were sprayed with EBR (0.4 mg/L) under light and darkness conditions (EBR + L, EBR + D), or sprayed with deionized water under light and darkness conditions as controls (L, D), at the onset of veraison...
January 9, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29314005/dynamics-of-oxygen-and-carbon-dioxide-in-rhizospheres-of-lobelia-dortmanna-a-planar-optode-study-of-belowground-gas-exchange-between-plants-and-sediment
#19
Nikola Lenzewski, Peter Mueller, Robert Johannes Meier, Gregor Liebsch, Kai Jensen, Ketil Koop-Jakobsen
Root-mediated CO2 uptake, O2 release and their effects on O2 and CO2 dynamics in the rhizosphere of Lobelia dortmanna were investigated. Novel planar optode technology, imaging CO2 and O2 distribution around single roots, provided insights into the spatiotemporal patterns of gas exchange between roots, sediment and microbial community. In light, O2 release and CO2 uptake were pronounced, resulting in a distinct oxygenated zone (radius: c. 3 mm) and a CO2 -depleted zone (radius: c. 2 mm) around roots. Simultaneously, however, microbial CO2 production was stimulated within a larger zone around the roots (radius: c...
January 4, 2018: New Phytologist
https://www.readbyqxmd.com/read/29313982/layer-specific-e-i-balances-during-neuronal-synchronization-in-the-visual-cortex
#20
Hillel Adesnik
Rhythmic activity can synchronize neural ensembles within and across cortical layers. While gamma band rhythmicity has been observed in all layers, the laminar sources and functional impacts of neuronal synchronization in the cortex remain incompletely understood. Here, layer-specific optogenetic stimulation demonstrates that populations of excitatory neurons in any cortical layer of the mouse's primary visual cortex are sufficient to powerfully entrain neuronal oscillations in the gamma band. Within each layer, inhibition balances excitation and keeps activity in check...
January 5, 2018: Journal of Physiology
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