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https://www.readbyqxmd.com/read/28822854/involvement-of-cyclic-nucleotide-gated-channels-in-spontaneous-activity-generated-in-isolated-interstitial-cells-of-cajal-from-the-rabbit-urethra
#1
Maria Sancho, Eamonn Bradley, Angeles Garcia-Pascual, Domingo Triguero, Keith D Thornbury, Mark A Hollywood, Gerard P Sergeant
Cyclic nucleotide-gated (CNG) channels are non-selective cation channels that mediate influx of extracellular Na(+) and Ca(2+) in various cell types. L-cis-Diltiazem, a CNG channel blocker, inhibits contraction of urethral smooth muscle (USM), however the mechanisms underlying this effect are still unclear. We investigated the possibility that CNG channels contribute to spontaneous pacemaker activity in freshly isolated interstitial cells of Cajal (ICC) isolated from the rabbit urethra (RUICC). Using immunocytochemistry, we found intense CNG1-immunoreactivity in vimentin-immunoreactive RUICC, mainly within patches of the cellular body and processes...
August 16, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28822586/intravenous-mannitol-versus-placebo-during-partial-nephrectomy-in-patients-with-normal-kidney-function-a-double-blind-clinically-integrated-randomized-trial
#2
Massimiliano Spaliviero, Nicholas E Power, Katie S Murray, Daniel D Sjoberg, Nicole E Benfante, Melanie L Bernstein, James Wren, Paul Russo, Jonathan A Coleman
BACKGROUND: Mannitol is currently used as a renal protective agent to mitigate the effects of renal ischemia during nephron-sparing surgery (NSS). This routine practice lacks rigorous methodological study. OBJECTIVE: To assess the effect on renal function outcomes after surgery of mannitol infusion prior to renal ischemia during NSS. DESIGN, SETTING, PARTICIPANTS: This prospective, randomized, placebo-controlled, double-blind trial included 199 patients with a preoperative estimated glomerular filtration rate (eGFR) >45ml/min/1...
August 16, 2017: European Urology
https://www.readbyqxmd.com/read/28821660/contribution-of-astroglial-cx43-hemichannels-to-the-modulation-of-glutamatergic-currents-by-d-serine-in-the-mouse-prefrontal-cortex
#3
Claire Meunier, Nan Wang, Chenju Yi, Glenn Dallerac, Pascal Ezan, Annette Koulakoff, Luc Leybaert, Christian Giaume
Astrocytes interact dynamically with neurons by modifying synaptic activity and plasticity. This interplay occurs through a process named gliotransmission, meaning that neuroactive molecules are released by astrocytes. Acting as a gliotransmitter, D-serine co-agonist of the NMDA receptor at the glycine binding site, can be released by astrocytes in a calcium [Ca(2+)]i-dependent manner. A typical feature of astrocytes is their high expression level of connexin43 (Cx43), a protein forming gap junction channels and hemichannels associated with dynamic neuroglial interactions...
August 11, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28815011/genomic-upregulation-of-cardiac-cav1-2%C3%AE-and-ncx1-by-estrogen-in-women
#4
Rita Papp, Glenna C L Bett, Agnieszka Lis, Randall L Rasmusson, István Baczkó, András Varró, Guy Salama
BACKGROUND: Women have a higher risk of lethal arrhythmias than men in long QT syndrome type 2 (LQTS2), but the mechanisms remain uncertain due to the limited availability of healthy control human tissue. We have previously reported that in female rabbits, estrogen increases arrhythmia risk in drug-induced LQTS2 by upregulating L-type Ca(2+) (ICa,L) and sodium-calcium exchange (INCX) currents at the base of the epicardium by a genomic mechanism. This study investigates if the effects of estrogen on rabbit ICa,L and INCX apply to human hearts...
2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28810599/effects-of-niflumic-acid-on-%C3%AE-aminobutyric-acid-induced-currents-in-isolated-dorsal-root-ganglion-neurons-of-neuropathic-pain-rats
#5
Li-Jie Wang, Yang Wang, Meng-Jie Chen, Zhen-Pu Tian, Bi-Han Lu, Ke-Tao Mao, Liang Zhang, Lei Zhao, Li-Ya Shan, Li Li, Jun-Qiang Si
Niflumic acid (NFA) is a type of non-steroidal anti-inflammatory drug. Neuropathic pain is caused by a decrease in presynaptic inhibition mediated by γ-aminobutyric acid (GABA). In the present study, a whole-cell patch-clamp technique and intracellular recording were used to assess the effect of NFA on GABA-induced inward current in dorsal root ganglion (DRG) neurons of a chronic constriction injury (CCI) model. It was observed that 1-1,000 µmol/l GABA induced a concentration-dependent inward current in DRG neurons...
August 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28809765/cyclin-dependent-kinase-5-modulates-the-p2x2a-receptor-channel-gating-through-phosphorylation-of-c-terminal-threonine-372
#6
Claudio Coddou, Rodrigo Sandoval, Patricio Castro, Pablo Lazcano, Maria José Hevia, Milos Rokic, Bradford Hall, Anita Terse, Christian Gonzalez-Billault, Ashok B Kulkarni, Stanko S Stojilkovic, Elias Utreras
The purinergic P2X2 receptor (P2X2R) is an adenosine triphosphate-gated ion channel widely expressed in the nervous system. Here, we identified a putative cyclin-dependent kinase 5 (Cdk5) phosphorylation site in the full-size variant P2X2aR (TPKH), which is absent in the splice variant P2X2bR. We therefore investigated the effects of Cdk5 and its neuronal activator, p35, on P2X2aR function. We found an interaction between P2X2aR and Cdk5/p35 by co-immunofluorescence and co-immunoprecipitation in HEK293 cells...
July 17, 2017: Pain
https://www.readbyqxmd.com/read/28809642/fibular-strut-graft-for-posterior-pelvic-ring-nonunion-a-case-report
#7
M Frank, J R McCallum, T Dědek
Posttraumatic pelvic nonunions in combination with malposition are uncommon in the present-day era of modern pelvic surgery. The case describes a new surgical technique for treatment of the nonunion localized to iliosacral joint. A 42-year-old polytraumatized male presented with a pelvic fracture (type 61-C2.3) associated with a complex acetabular fracture. The patient was treated and the pelvis stabilized according to damage control principles (external fixation and pelvic C-clamp) and subsequently definitively treated according to principles of current pelvic surgery...
2017: Acta Chirurgiae Orthopaedicae et Traumatologiae Cechoslovaca
https://www.readbyqxmd.com/read/28808020/kcne1-and-kcne3-modulate-kcnq1-channels-by-affecting-different-gating-transitions
#8
Rene Barro-Soria, Rosamary Ramentol, Sara I Liin, Marta E Perez, Robert S Kass, H Peter Larsson
KCNE β-subunits assemble with and modulate the properties of voltage-gated K(+) channels. In the heart, KCNE1 associates with the α-subunit KCNQ1 to generate the slowly activating, voltage-dependent potassium current (IKs) in the heart that controls the repolarization phase of cardiac action potentials. By contrast, in epithelial cells from the colon, stomach, and kidney, KCNE3 coassembles with KCNQ1 to form K(+) channels that are voltage-independent K(+) channels in the physiological voltage range and important for controlling water and salt secretion and absorption...
August 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28807015/genomic-upregulation-of-cardiac-cav1-2%C3%AE-and-ncx1-by-estrogen-in-women
#9
Rita Papp, Glenna C L Bett, Agnieszka Lis, Randall L Rasmusson, István Baczkó, András Varró, Guy Salama
BACKGROUND: Women have a higher risk of lethal arrhythmias than men in long QT syndrome type 2 (LQTS2), but the mechanisms remain uncertain due to the limited availability of healthy control human tissue. We have previously reported that in female rabbits, estrogen increases arrhythmia risk in drug-induced LQTS2 by upregulating L-type Ca(2+) (ICa,L) and sodium-calcium exchange (INCX) currents at the base of the epicardium by a genomic mechanism. This study investigates if the effects of estrogen on rabbit ICa,L and INCX apply to human hearts...
August 14, 2017: Biology of Sex Differences
https://www.readbyqxmd.com/read/28805971/anticonvulsive-evaluation-of-thip-in-the-murine-pentylenetetrazole-kindling-model-lack-of-anticonvulsive-effect-of-thip-despite-functional-%C3%AE-subunit-containing-gabaa-receptors-in-dentate-gyrus-granule-cells
#10
Charlotte Simonsen, Kim Boddum, Nadia L von Schoubye, Alissa Kloppenburg, Kasper Sønderskov, Suzanne L Hansen, Uffe Kristiansen
THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) is a GABAA receptor agonist with varying potencies and efficacies at γ-subunit-containing receptors. More importantly, THIP acts as a selective superagonist at δ-subunit-containing receptors (δ-GABAA Rs) at clinically relevant concentrations. Evaluation of THIP as a potential anticonvulsant has given contradictory results in different animal models and for this reason, we reevaluated the anticonvulsive properties of THIP in the murine pentylenetetrazole (PTZ) kindling model...
August 2017: Pharmacology Research & Perspectives
https://www.readbyqxmd.com/read/28805023/onion-allium-cepa-l-peel-extract-ope-regulates-human-sperm-motility-via-protein-kinase-c-mediated-activation-of-the-human-voltage-gated-proton-channel
#11
M R Chae, S J Kang, K P Lee, B R Choi, H K Kim, J K Park, C Y Kim, S W Lee
Onion (Allium cepa L.) and quercetin protect against oxidative damage and have positive effects on multiple functional parameters of spermatozoa, including viability and motility. However, the associated underlying mechanisms of action have not yet been identified. The aim of this study was to investigate the effect of onion peel extract (OPE) on voltage-gated proton (Hv1) channels, which play a critical role in rapid proton extrusion. This process underlies a wide range of physiological processes, particularly male fertility...
August 13, 2017: Andrology
https://www.readbyqxmd.com/read/28802903/role-of-canonical-transient-receptor-potential-channel-3-in-acetylcholine-induced-mouse-airway-smooth-muscle-cell-proliferation
#12
Xiao-Xu Chen, Jia-Hua Zhang, Bin-Hua Pan, Hui-Li Ren, Xiu-Ling Feng, Jia-Ling Wang, Jun-Hua Xiao
AIMS: Canonical transient receptor potential channel-3 (TRPC3)-encoded Ca(2+)-permeable nonselective cation channel (NSCC) has been proven to be an important native constitutively active channel in airway smooth muscle cell (ASMC), which plays significant roles in physiological and pathological conditions by controlling Ca(2+) homeostasis in ASMC. Acetylcholine (ACh) is generally accepted as a contractile parasympathetic neurotransmitter in the airway. Recently studies have revealed the pathological role of ACh in airway remodeling, however, the mechanisms remain unclear...
August 9, 2017: Life Sciences
https://www.readbyqxmd.com/read/28801600/cav1-3-l-type-ca-2-channel-contributes-to-the-heartbeat-by-generating-a-dihydropyridine-sensitive-persistent-na-current
#13
Futoshi Toyoda, Pietro Mesirca, Stefan Dubel, Wei-Guang Ding, Joerg Striessnig, Matteo E Mangoni, Hiroshi Matsuura
The spontaneous activity of sinoatrial node (SAN) pacemaker cells is generated by a functional interplay between the activity of ionic currents of the plasma membrane and intracellular Ca(2+) dynamics. The molecular correlate of a dihydropyridine (DHP)-sensitive sustained inward Na(+) current (I st), a key player in SAN automaticity, is still unknown. Here we show that I st and the L-type Ca(2+) current (I Ca,L) share CaV1.3 as a common molecular determinant. Patch-clamp recordings of mouse SAN cells showed that I st is activated in the diastolic depolarization range, and displays Na(+) permeability and minimal inactivation and sensitivity to I Ca,L activators and blockers...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800884/ionic-mechanisms-of-the-action-of-anaesthetics-on-sinoatrial-node-automaticity
#14
REVIEW
Akiko Kojima, Hiroshi Matsuura
Although various general anaesthetics affect the heart rate in clinical settings, their precise mechanisms remain to be fully elucidated. Because the heart rate is determined by automaticity of the cardiac pacemaker sinoatrial node and its regulation by autonomic nervous system, it is important to clarify the effect of anaesthetics on sinoatrial node automaticity. The spontaneous electrical activity of sinoatrial node is generated by a complex but coordinated interaction of multiple ionic currents, such as the hyperpolarisation-activated cation current (If), T-type and L-type Ca(2+) currents (ICa,T and ICa,L), Na(+)/Ca(2+) exchange current (INCX), and rapidly and slowly activating delayed rectifier K(+) currents (IKr and IKs)...
August 8, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28798669/receptor-mechanisms-mediating-the-pro-nociceptive-action-of-hydrogen-sulfide-in-rat-trigeminal-neurons-and-meningeal-afferents
#15
Kseniya Koroleva, Alsu Mustafina, Aleksey Yakovlev, Anton Hermann, Rashid Giniatullin, Guzel Sitdikova
Hydrogen sulfide (H2S), a well-established member of the gasotransmitter family, is involved in a variety of physiological functions, including pro-nociceptive action in the sensory system. Although several reports have shown that H2S activates sensory neurons, the molecular targets of H2S action in trigeminal (TG) nociception, implicated in migraine, remains controversial. In this study, using suction electrode recordings, we investigate the effect of the H2S donor, sodium hydrosulfide (NaHS), on nociceptive firing in rat meningeal TG nerve fibers...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28780056/chemokine-ligand-2-chemokine-receptor-2-signaling-in-the-trigeminal-ganglia-contributes-to-inflammatory-hyperalgesia-in-rats
#16
Mamoru Takeda, Masanori Nasu, Takuya Kanazawa, Masayuki Takahashi, Yoshihito Shimazu
This study investigated the functional significance of hyperalgesia in the CCL2/CCR2 signaling system in trigeminal ganglion (TG) neurons following inflammation. Inflammation was induced by injection of complete Freund's adjuvant (CFA) into the whisker pad of rats. The escape threshold from mechanical stimulation applied to the whisker pad 2days later was significantly lower in CFA-treated rats than in naïve rats. Fluorogold (FG) labeling was used to identify the TG neurons innervating the whisker pad. FG-labeled TG neurons were immunoreactive for CCL2/CCR2...
August 2, 2017: Neuroscience Research
https://www.readbyqxmd.com/read/28774158/regulation-of-intracellular-calcium-by-endoplasmic-reticulum-proteins-in-small-intestinal-interstitial-cells-of-cajal
#17
Chan Guk Park, Mei Jin Wu, Chansik Hong, Ju Yeon Jo, Han Yi Jiao, Hyun Park, Jae Yeoul Jun, Seok Choi
Background/Aims: We investigated the role of representative endoplasmic reticulum proteins, stromal interaction molecule 1 (STIM1), and store-operated calcium entry-associated regulatory factor (SARAF) in pacemaker activity in cultured interstitial cells of Cajal (ICCs) isolated from mouse small intestine. Methods: The whole-cell patch clamp technique applied for intracellular calcium ions ([Ca(2+)]i) analysis with STIM1 or SARAF overexpressed cultured ICCs from mouse small intestine...
August 4, 2017: Journal of Neurogastroenterology and Motility
https://www.readbyqxmd.com/read/28771534/neural-stem-cell-mediated-recovery-is-enhanced-by-chondroitinase-abc-pretreatment-in-chronic-cervical-spinal-cord-injury
#18
Hidenori Suzuki, Christopher S Ahuja, Ryan P Salewski, Lijun Li, Kajana Satkunendrarajah, Narihito Nagoshi, Shinsuke Shibata, Michael G Fehlings
Traumatic spinal cord injuries (SCIs) affect millions of people worldwide; the majority of whom are in the chronic phase of their injury. Unfortunately, most current treatments target the acute/subacute injury phase as the microenvironment of chronically injured cord consists of a well-established glial scar with inhibitory chondroitin sulfate proteoglycans (CSPGs) which acts as a potent barrier to regeneration. It has been shown that CSPGs can be degraded in vivo by intrathecal Chondroitinase ABC (ChABC) to produce a more permissive environment for regeneration by endogenous cells or transplanted neural stem cells (NSCs) in the subacute phase of injury...
2017: PloS One
https://www.readbyqxmd.com/read/28769585/%C3%AE-lipoic-acid-suppresses-neuronal-excitability-and-attenuates-colonic-hypersensitivity-to-colorectal-distention-in-diabetic-rats
#19
Yan Sun, Pan-Pan Yang, Zhen-Yuan Song, Yu Feng, Duan-Min Hu, Ji Hu, Guang-Yin Xu, Hong-Hong Zhang
AIM: Patients with long-standing diabetes often demonstrate intestinal dysfunction, characterized as constipation or colonic hypersensitivity. Our previous studies have demonstrated the roles of voltage-gated sodium channels NaV1.7 and NaV1.8 in dorsal root ganglion (DRG) in colonic hypersensitivity of rats with diabetes. This study was designed to determine roles of antioxidant α-lipoic acid (ALA) on sodium channel activities and colonic hypersensitivity of rats with diabetes. METHODS: Streptozotocin was used to induce diabetes in adult female rats...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28768052/cardioprotective-effects-of-5-hydroxymethylfurfural-mediated-by-inhibition-of-l-type-ca-2-current
#20
G Wölkart, A Schrammel, C N Koyani, S Scherübel, K Zorn-Pauly, E Malle, B Pelzmann, M Andrä, A Ortner, B Mayer
BACKGROUND AND PURPOSE: The antioxidant 5-hydroxymethylfurfural (5-HMF) exerts documented beneficial effects in several experimental pathologies and is currently tested as antisickling drug in clinical trials. In the present study we examined the cardiovascular effects of 5-HMF and elucidated the mode of action of the drug. EXPERIMENTAL APPROACH: The cardiovascular effects of 5-HMF were studied with precontracted porcine coronary arteries and isolated normoxic perfused rat hearts...
August 2, 2017: British Journal of Pharmacology
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