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https://www.readbyqxmd.com/read/28737182/micro-and-nano-patterned-elastin-like-polypeptide-hydrogels-for-stem-cell-culture
#1
A Paul, M Stührenberg, S Chen, D Rhee, W-K Lee, T W Odom, S C Heilshorn, A Enejder
We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy...
July 24, 2017: Soft Matter
https://www.readbyqxmd.com/read/28736601/classification-review-of-dental-adhesive-systems-from-the-iv-generation-to-the-universal-type
#2
REVIEW
Eshrak Sofan, Afrah Sofan, Gaspare Palaia, Gianluca Tenore, Umberto Romeo, Guido Migliau
Adhesive dentistry has undergone great progress in the last decades. In light of minimal-invasive dentistry, this new approach promotes a more conservative cavity design, which relies on the effectiveness of current enamel-dentine adhesives. Adhesive dentistry began in 1955 by Buonocore on the benefits of acid etching. With changing technologies, dental adhesives have evolved from no-etch to total-etch (4(th) and 5(th) generation) to self-etch (6(th), 7(th) and 8(th) generation) systems. Currently, bonding to dental substrates is based on three different strategies: 1) etch-and-rinse, 2) self-etch and 3) resin-modified glass-ionomer approach as possessing the unique properties of self-adherence to the tooth tissue...
January 2017: Annali di Stomatologia
https://www.readbyqxmd.com/read/28735626/differentiation-of-hmsc-and-hpdlsc-induced-by-pge2-or-bmp-7-in-3d-models
#3
Christina Ern, Tamara Berger, Iris Frasheri, Richard Heym, Reinhard Hickel, Matthias Folwaczny
Regenerative therapies of pathogenic tissue defects are gaining increasing importance in periodontology. Among others, the osteogenic effect of BMP-7 seems to play a major role in the development of teeth and alveolar bone. Human periodontal ligament stem cells (hPDLSC), as well as human mesenchymal stem cells (hMSC), show the ability to differentiate into various types of tissues. Regarding prostaglandin E2, many studies have confirmed that it is involved in the inflammation associated to periodontitis stimulating osteoclasts, which ultimately leads to resorption of tooth supporting bone...
July 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28735148/mechanically-competent-and-cytocompatible-polycaprolactone-borophosphosilicate-hybrid-biomaterials
#4
Dibakar Mondal, S Jeffrey Dixon, Kibret Mequanint, Amin S Rizkalla
Organic-inorganic class II hybrid materials have domain sizes at the molecular level and chemical bonding between the organic and inorganic phases. We have previously reported the synthesis of class II hybrid biomaterials from alkoxysilane-functionalized polycaprolactone (PCL) and borophosphosilicate (B2O3-P2O5-SiO2) glass (BPSG) through a non-aqueous sol-gel process. In the present study, the mechanical properties and degradability of these PCL/BPSG hybrid biomaterials were studied and compared to those of their conventional composite counterparts...
July 5, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28735029/peptide-modification-of-polyimide-insulated-microwires-towards-improved-biocompatibility-through-reduced-glial-scarring
#5
Sangita Sridar, Matthew A Churchward, Vivian K Mushahwar, Kathryn G Todd, Anastasia L Elias
The goal of this study is to improve the integration of implanted microdevices with tissue in the central nervous system (CNS). The long-term utility of neuroprosthetic devices implanted in the CNS is affected by the formation of a scar by resident glial cells (astrocytes and microglia), limiting the viability and functional stability of the devices. Reduction in the proliferation of glial cells is expected to enhance the biocompatibility of devices. We demonstrate the modification of polyimide-insulated microelectrodes with a bioactive peptide KHIFSDDSSE...
July 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28734973/minimally-invasive-approaches-to-myoma-management
#6
REVIEW
Julian A Gingold, Ndeye-Aicha Gueye, Tommaso Falcone
Patients affected by the presence of leiomyomas may incur a substantial physical, emotional, social and financial toll as well as losses in their quality of life. Although many myomas are not amenable to medical therapy or hysteroscopic resection, many others are amenable to minimally invasive surgical approaches. In patients who prefer to retain their fertility, laparoscopic myomectomy should be considered the intervention of choice. In this review we expand on the surgical techniques of both conventional laparoscopic and robotic-assisted myomectomies...
July 19, 2017: Journal of Minimally Invasive Gynecology
https://www.readbyqxmd.com/read/28734346/hap-nanoparticle-and-substrate-surface-electrical-potential-towards-bone-cells-adhesion-recent-results-review
#7
REVIEW
Vladimir Bystrov, Anna Bystrova, Yuri Dekhtyar
Nanostructured hydroxyapatite (HAP) and its nanoparticles are widely used for implantation into the human organism. The biocompatibility of the implants depends very much on the interaction between the implant and the cells regenerating tissue to be connected to the implant. An implant surface electrical charged density plays an important role in these processes. Possible instruments managing the surface electrical potential of HAP are in the focus of this paper. Both theoretical and experimental results evidence that: - the surface electrical charge density of the nanoparticle depends on its size and shape; - the electrical charge density of HAP could be engineered by contact less technique because of deposition of the electrical charge from the external radiation source, surface couples reconstruction...
May 3, 2017: Advances in Colloid and Interface Science
https://www.readbyqxmd.com/read/28733347/ultrastructural-characteristics-and-immune-profile-of-equine-mscs-from-fetal-adnexa
#8
Eleonora Iacono, Luisa Pascucci, Barbara Rossi, Cinzia Bazzucchi, Aliai Lanci, Monica Ceccoli, Barbara Merlo
Both in human and equine species, mesenchymal stem cells (MSCs) from amniotic membrane (AM) and Wharton's jelly (WJ), may be particularly useful for immediate use or in later stages of life, after cryopreservation in cell bank. The aim of this study was to compare equine AM- and WJ-MSCs in vitro features, that may be relevant for their clinical employment. MSCs were more easily isolated from WJ, even if MSCs derived from AM exhibited more rapid proliferation (P<0.05). Osteogenic and chondrogenic differentiation were more prominent in MSCs derived from WJ...
July 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28733146/resveratrol-efficiently-improves-pulmonary-function-via-stabilizing-mast-cells-in-a-rat-intestinal-injury-model
#9
Xiaolei Huang, Weicheng Zhao, Dan Hu, Xue Han, Hanbin Wang, Jianyu Yang, Yang Xu, Yuantao Li, Weifeng Yao, Chaojin Chen
BACKGROUND: Intestinal ischemia/reperfusion (IIR) leads to acute lung injury (ALI) distally by aggravating pulmonary oxidative stress. Resveratrol is effective in attenuating ALI through its antioxidant capacity. This study aimed to determine the effects of resveratrol on IIR-induced ALI and to explore the role of mast cells (MCs) activation in a rat model of IIR. METHODS: Adult Sprague-Dawley rats were subjected to IIR by occluding the superior mesenteric artery for 60min followed by 4-hour reperfusion...
July 18, 2017: Life Sciences
https://www.readbyqxmd.com/read/28732919/injectable-thermosensitive-hydrogel-containing-hyaluronic-acid-and-chitosan-as-a-barrier-for-prevention-of-postoperative-peritoneal-adhesion
#10
Chih-Hao Chen, Shih-Hsien Chen, Shih-Hsuan Mao, Ming-Jin Tsai, Pang-Yun Chou, Chien-Hung Liao, Jyh-Ping Chen
Peritoneal adhesion is one of the common complications after abdominal surgery. Injectable thermosensitive hydrogel could serve as an ideal barrier to prevent this postoperative tissue adhesion. In this study, poly(N-isopropylacrylamide) (PNIPAm) was grafted to chitosan (CS) and the polymer was further conjugated with hyaluronic acid (HA) to form thermosensitive HA-CS-PNIPAm hydrogel. Aqueous solutions of PNIPAm and HA-CS-PNIPAm at 10%(w/v) are both free-flowing and injectable at room temperature and exhibit sol-gel phase transition around 31°C; however, HA-CS-PNIPAm shows less volume shrinkage after gelation and higher complex modulus than PNIPAm...
October 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28731483/the-role-of-laminins-in-cartilaginous-tissues-from-development-to-regeneration
#11
Y Sun, T L Wang, W S Toh, M Pei
As a key molecule of the extracellular matrix, laminin provides a delicate microenvironment for cell functions. Recent findings suggest that laminins expressed by cartilage-forming cells (chondrocytes, progenitor cells and stem cells) could promote chondrogenesis. However, few papers outline the effect of laminins on providing a favorable matrix microenvironment for cartilage regeneration. In this review, we delineated the expression of laminins in hyaline cartilage, fibrocartilage and cartilage-like tissue (nucleus pulposus) throughout several developmental stages...
July 21, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/28731325/bioinspired-and-biomimetic-agnps-gentamicin-embedded-silk-fibroin-coatings-for-robust-antibacterial-and-osteogenetic-applications
#12
Wenhao Zhou, Zhaojun Jia, Pan Xiong, Jianglong Yan, Yangyang Li, Ming Li, Yan Cheng, Yufeng Zheng
With the progressively increasing demand for orthopedic Ti implants, the balance realization between two primary complications restricting implants applications is imperative needed to be solved that the lack of bone tissue integration and biomedical device-associated infections (BAI), where emergence of multi-resistance bacteria make it worse. Notably, a combination of silver nanoparticles (AgNPs) and a kind of antibiotic can synergistically inhibit bacterial growth, where low concentration of AgNPs has been confirmed to promote the proliferation and osteogenesis of osteoblasts...
July 21, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28730545/platelet-first-responders-in-wound-response-cancer-and-metastasis
#13
REVIEW
David G Menter, Scott Kopetz, Ernest Hawk, Anil K Sood, Jonathan M Loree, Paolo Gresele, Kenneth V Honn
Platelets serve as "first responders" during normal wounding and homeostasis. Arising from bone marrow stem cell lineage megakaryocytes, anucleate platelets can influence inflammation and immune regulation. Biophysically, platelets are optimized due to size and discoid morphology to distribute near vessel walls, monitor vascular integrity, and initiate quick responses to vascular lesions. Adhesion receptors linked to a highly reactive filopodia-generating cytoskeleton maximizes their vascular surface contact allowing rapid response capabilities...
July 20, 2017: Cancer Metastasis Reviews
https://www.readbyqxmd.com/read/28730511/intermediate-molecular-mass-hyaluronan-and-cd44-receptor-interactions-enhance-neutrophil-phagocytosis-and-il-8-production-via-p38-and-erk1-2-mapk-signalling-pathways
#14
Cheng-Hsun Lu, Chia-Huei Lin, Ko-Jen Li, Chieh-Yu Shen, Cheng-Han Wu, Yu-Min Kuo, Ting-Syuan Lin, Chia-Li Yu, Song-Chou Hsieh
CD44 is a common leukocyte adhesion molecule expressed on the surface of various cells. Hyaluronan (HA), the natural ligand of CD44, is a simple repeated disaccharide with variable molecular mass that is widely distributed on cell surfaces and the connective tissue matrix. The binding of small molecular mass HA (SMM-HA, MW < 80 kDa) to CD44 on immune-related cells elicits cell proliferation, differentiation, and cytokine production. However, the effects and molecular basis of intermediate molecular mass HA (IMM-HA, MW ≈ 500 kDa)-CD44 interactions on polymorphonuclear neutrophil (PMN) functions have not been elucidated...
July 20, 2017: Inflammation
https://www.readbyqxmd.com/read/28730091/aberrant-expression-of-genes-and-proteins-in-pterygium-and-their-implications-in-the-pathogenesis
#15
REVIEW
Qing-Yang Feng, Zi-Xuan Hu, Xi-Ling Song, Hong-Wei Pan
Pterygium is a common ocular surface disease induced by a variety of factors. The exact pathogenesis of pterygium remains unclear. Numbers of genes and proteins are discovered in pterygium and they function differently in the occurrence and development of this disease. We searched the Web of Science and PubMed throughout history for literatures about the subject. The keywords we used contain pterygium, gene, protein, angiogenesis, fibrosis, proliferation, inflammation, pathogenesis and therapy. In this review, we summarize the aberrant expression of a range of genes and proteins in pterygium compared with normal conjunctiva or cornea, including growth factors, matrix metalloproteinases and tissue inhibitors of metalloproteinases, interleukins, tumor suppressor genes, proliferation related proteins, apoptosis related proteins, cell adhesion molecules, extracellular matrix proteins, heat shock proteins and tight junction proteins...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28729806/evidence-provided-for-the-use-of-oscillating-instruments-in-restorative-dentistry-a-systematic-review
#16
REVIEW
Panagiotis Ntovas, Spyridon Doukoudakis, John Tzoutzas, Panagiotis Lagouvardos
Oscillating diamond instruments are considered gentle sources for the removal of demineralized tooth hard tissues and the preparation of cavity angles and margins needed in minimally invasive dentistry. However, there is a question if literature provides enough evidence for their efficacy in restorative dentistry procedures. A literature search until May 2016 was conducted, using PubMed, Scopus, and The Cochrane Central Register of Controlled Trials databases. The quality of the studies was assessed using the recommendation of the Oxford Centre for Evidence-based Medicine...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28729803/anterosuperior-rehabilitation-with-metal-free-fixed-prosthesis-based-on-zirconia
#17
Sthelen Nayara Cenci, Igor Alessandro Gontarsky, Marcella Goetz Moro, Lidia Olga Bach Pinheiro, Adriana Postiglione Bührer Samra
The loss of upper front dental elements causes functional and psychosocial problems to the affected individuals. In this case report, the treatment planning considered hard and soft tissue loss for a complex fixed partial denture (FPD) rehabilitation. The six-element, all-ceramic FPD was manufactured using a computer-aided design/computer-aided manufacturing system with zirconia framework, veneered with leucite-reinforced ceramic. Ceramic artificial gum was also produced to guarantee tooth-facial proportions as well as lip support, promoting both esthetics and phonetics...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28729217/preventing-postoperative-tissue-adhesion-using-injectable-carboxymethyl-cellulose-pullulan-hydrogels
#18
Sumi Bang, Young-Gwang Ko, Won Il Kim, Donghwan Cho, Won Ho Park, Oh Hyeong Kwon
An injectable adhesive hydrogel composed of carboxymethyl cellulose (CMC) and pullulan is developed and evaluated as a postoperative anti-adhesion barrier. CMC was modified with tyramine to introduce crosslinking via an EDC-NHS reaction. The in situ hydrogel was developed by an enzyme-mediated reaction of tyramine-immobilized CMC with horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Pullulan was added to the hydrogel solution to improve adhesiveness to the wound area and accelerate biodegradation...
July 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28728969/a-nanoporous-titanium-surface-promotes-the-maturation-of-focal-adhesions-and-the-formation-of-filopodia-with-distinctive-nanoscale-protrusions-by-osteogenic-cells
#19
Dainelys Guadarrama Bello, Aurélien Fouillen, Antonella Badia, Antonio Nanci
While topography is a key determinant of the cellular response to biomaterials the mechanisms implicated in the cell-surface interactions are complex and still not fully elucidated. In this context, we have examined the effect of nanoscale topography on the formation of filopodia, focal adhesions, and on gene expression of proteins associated with cell adhesion and sensing. Commercially pure titanium discs were treated for oxidative nanopatterning with a solution of H2SO4/H2O2 50:50 (v/v). Scanning electron microscopy and atomic force microscopy characterizations showed that this facile chemical treatment efficiently creates a unique nanoporous surface with a root-mean-square roughness of 11...
July 17, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28727052/rapid-communication-subclinical-bovine-respiratory-disease-loci-and-pathogens-associated-with-lung-lesions-in-feedlot-cattle
#20
J N Kiser, T E Lawrence, M Neupane, C M Seabury, J F Taylor, J E Womack, H L Neibergs
Bovine respiratory disease (BRD) is an economically important disease of feedlot cattle that is caused by viral and bacterial pathogen members of the BRD complex. Many cases of subclinical BRD go untreated and are not detected until slaughter, when lung lesions are identified. The objectives of this study were to identify which BRD pathogens were associated with the presence of lung lesions at harvest and to identify genomic loci that were associated with susceptibility to lung lesions as defined by consolidation of the lung and/or the presence of fibrin tissue...
June 2017: Journal of Animal Science
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