keyword
MENU ▼
Read by QxMD icon Read
search

Stat3

keyword
https://www.readbyqxmd.com/read/29679903/isoharringtonine-inhibits-breast-cancer-stem-like-properties-and-stat3-signaling
#1
Wei Chen, Hui Wang, Mei Cheng, Ling Ni, Li Zou, Qin Yang, Xianghai Cai, Baowei Jiao
OBJECTIVES: Breast cancer stem cells (BCSCs) contribute to breast cancer progression, relapse, and treatment resistance. Identification of the natural inhibitory components of BCSCs is therefore critical for clinical treatment. Here, we investigated whether isoharringtonine (IHT) had inhibitory effects on BCSCs in breast cancer cell lines. METHODS: HCC1806, HCC1937, and MCF7 cells were treated with IHT. The proliferation and the migration of cells were detected by MTS assay and wound healing migration assay, respectively...
April 18, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29679238/identification-of-subsets-of-actionable-genetic-alterations-in-kras-mutant-lung-cancers-using-association-rule-mining
#2
Junior Tayou
BACKGROUND: Lung cancer is the leading cause of cancer-related death in both men and women. KRAS mutations occur in ~ 25% of patients with lung cancer, and the presence of these mutations is associated with a poor prognosis. Unfortunately, efforts to directly target KRAS or its associated downstream MAPK or PI3K/AKT/mTOR pathways have seen little or no benefits. Here, I hypothesize that KRAS-mutant tumors do not respond to KRAS pathway therapies due to the co-occurrence of other activated cell survival pathways and/or mechanisms...
April 20, 2018: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29678906/rnai-screening-identifies-a-mechanosensitive-rock-jak2-stat3-network-central-to-myofibroblast-activation
#3
Raymond S Oh, Andrew J Haak, Karry M J Smith, Giovanni Ligresti, Kyoung Moo Choi, Tiao Xie, Shaohua Wang, Paula R Walters, Michael A Thompson, Michelle R Freeman, Logan J Manlove, Vivian M Chu, Carol Feghali-Bostwick, Anja C Roden, Jürgen Schymeinsky, Christina M Pabelick, Y S Prakash, Robert Vassallo, Daniel J Tschumperlin
Myofibroblasts play key roles in wound healing and pathologic fibrosis. Here we undertook an RNAi screen to characterize myofibroblast regulatory genes, using a high-content imaging approach to quantify alpha-smooth muscle actin stress fibers in cultured human fibroblasts. Screen hits were validated on physiological compliance hydrogels, and selected hits tested in primary fibroblasts from patients with idiopathic pulmonary fibrosis. Our RNAi screen led to the identification of STAT3 as an essential mediator of myofibroblast activation and function...
April 20, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29678628/poria-cocos-polysaccharide-attenuates-rankl-induced-osteoclastogenesis-by-suppressing-nfatc1-activity-and-phosphorylation-of-erk-and-stat3
#4
Dezhi Song, Zhen Cao, Jennifer Tickner, Heng Qiu, Chao Wang, Kai Chen, Ziyi Wang, Chunyu Guo, Shiwu Dong, Jiake Xu
Pathological fractures caused by osteolytic lesions seriously threaten the health of patients. Osteoclasts play important roles in bone resorption whose hyperfunction are closely related to osteolytic lesions. Studies on osteoclast differentiation and function assist in the prevention of excessive bone loss associated diseases. We screened a variety of natural compounds with anti-inflammatory effect and found that poria cocos polysaccharide (PCP) inhibited RANKL-induced osteoclast formation and bone resorption via TRAcP staining, immunofluorescence, RT-PCR and western blot...
April 17, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29678569/pbx1-promotes-the-cell-proliferation-via-jak2-stat3-signaling-in-clear-cell-renal-carcinoma
#5
Xin Wei, Lili Yu, Yi Li
PBX1 was abnormally overexpressed and its intracellular localization was found to be frequently amplified in many types of cancer, including renal clear cell carcinoma. PBX1 displays oncogenic activity in several different types of cells, but little is known about how signaling pathways are altered, and the function of PBX1 in renal clear cell carcinoma has not been well investigated. In this study, we demonstrate that the expression of PBX1 was significantly upregulated in 30 pairs of human tissues compared to adjacent normal tissues and the overall survival rate of PBX1-high group was significantly worse than that of PBX1-low group...
April 17, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29677549/mir-375-prevents-nasal-mucosa-cells-from-apoptosis-and-ameliorates-allergic-rhinitis-via-inhibiting-jak2-stat3-pathway
#6
Tao Wang, Dong Chen, Peihua Wang, Zhou Xu, Ying Li
This study aims to explore the roles, and related mechanisms of miR-375 in nasal mucosa cells apoptosis and allergic rhinitis. Here, miR-375 was found to be decreased in the epithelia of nasal mucosa of allergic rhinitis mice and TNF-α-stimulated nasal mucosa cells, while JAK2 expression exhibited an opposite effect. Mechanistically, miR-375/JAK2 regulatory axis was identified in nasal mucosa cells via luciferase reporter, qRT-PCR, western blot and RNA immune co-precipitation (RIP) assays. Pre- or post-injection of miR-375 agomir following ovalbumin (OVA) treatment attenuated OVA-induced allergic rhinitis, characterized as the downregulation of IL-6 and TNF-α secretion, and upregulation of IL-10 secretion, these effects were attenuated by infection with JAK2 adenovirus through nasal cavity inhalation...
April 17, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29677532/sulforaphane-promotes-apoptosis-and-inhibits-proliferation-and-self-renewal-of-nasopharyngeal-cancer-cells-by-targeting-stat-signal-through-mirna-124-3p
#7
Xiqing Li, Zunlan Zhao, Ming Li, Mingyue Liu, Andres Bahena, Yaping Zhang, Yuwei Zhang, Chetan Nambiar, Guangzhi Liu
Sulforaphane (SF) exhibits an anti-tumor effect in a variety of cancers, but little is known about its function in nasopharyngeal carcinoma. SF could decrease the expression of stem cell markers, β-catenin, Nanog, c-Myc, Oct3/4 and Sox2 in nasopharyngeal cancer cells (HONE1 and SUN1), and inhibit the formation of tumor spheres. Moreover, SF inhibits proliferation and induces apoptosis decreasing the stemness of nasopharyngeal cancer cells through a mechanism related to STAT3 signaling in vitro. We found that SF inhibits total STAT3 expression level and STAT3 phosphorylation (troy 704 and troy 705) by upregulation of miRNA-124-3p...
April 16, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29675106/overexpression-of-klotho-inhibits-helf-fibroblasts-sasp-related-protumoral-effects-on-non-small-cell-lung-cancer-cells
#8
Bo Chen, Yan Liang, Liben Chen, Yunyan Wei, Yue Li, Weihong Zhao, Jianqing Wu
Lung cancer (LC) is the most common cause of death from cancer worldwide, and it is also a closely aging-related disease. Klotho, a new anti-aging gene, has been proven to play a critical role in regulating aging and the development of age-related diseases including LC. However, whether Klotho is a key link between aging and LC is still unknown. Here we report that Klotho can indirectly inhibit LC growth and development through regulating senescence-associated secretory phenotype (SASP). We found that senescent lung fibroblasts (SLF) can promote production of IL-6 and IL-8, which can be effectively inhibited by overexpressing Klotho...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29674644/aggressive-natural-killer-cell-leukemia-mutational-landscape-and-drug-profiling-highlight-jak-stat-signaling-as-therapeutic-target
#9
Olli Dufva, Matti Kankainen, Tiina Kelkka, Nodoka Sekiguchi, Shady Adnan Awad, Samuli Eldfors, Bhagwan Yadav, Heikki Kuusanmäki, Disha Malani, Emma I Andersson, Paavo Pietarinen, Leena Saikko, Panu E Kovanen, Teija Ojala, Dean A Lee, Thomas P Loughran, Hideyuki Nakazawa, Junji Suzumiya, Ritsuro Suzuki, Young Hyeh Ko, Won Seog Kim, Shih-Sung Chuang, Tero Aittokallio, Wing C Chan, Koichi Ohshima, Fumihiro Ishida, Satu Mustjoki
Aggressive natural killer-cell (NK-cell) leukemia (ANKL) is an extremely aggressive malignancy with dismal prognosis and lack of targeted therapies. Here, we elucidate the molecular pathogenesis of ANKL using a combination of genomic and drug sensitivity profiling. We study 14 ANKL patients using whole-exome sequencing (WES) and identify mutations in STAT3 (21%) and RAS-MAPK pathway genes (21%) as well as in DDX3X (29%) and epigenetic modifiers (50%). Additional alterations include JAK-STAT copy gains and tyrosine phosphatase mutations, which we show recurrent also in extranodal NK/T-cell lymphoma, nasal type (NKTCL) through integration of public genomic data...
April 19, 2018: Nature Communications
https://www.readbyqxmd.com/read/29674294/discovery-of-new-benzensulfonamide-derivatives-as-tripedal-stat3-inhibitors
#10
Jianpeng Guo, Wenying Yu, Guiping Cai, Wenda Zhang, Shanshan Li, Jiawen Zhu, Dongmei Song, Lingyi Kong
Persistent activated STAT3 has a striking correlation with cancer development and inhibition of STAT3 signaling pathway is a novel therapeutic way for human cancers. Among STAT family, STAT1 and STAT3 play opposite roles in tumorigenesis. However, the discovery of selective STAT3 inhibitors is still challenging to date. In this study, a series of small-molecular (MW < 500) benzensulfanilamide derivatives were designed to selectively suppress STAT3 activation for anti-cancer treatment. The most potent compound 11 inhibited both overexpressed and IL-6 induced STAT3 phosphorylation, whereas 11 displayed little effect on the phosphorylation of other STAT isoforms STAT1, STAT5, demonstrating 11 was a selective STAT3 inhibitor...
April 4, 2018: European Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/29673911/targeting-the-rhoa-rock-pathway-to-regulate-t-cell-homeostasis-in-hypoxia-induced-pulmonary-arterial-hypertension
#11
Cheng Li, Ping-Ping Liu, Dou-Dou Tang, Rong Song, Yi-Qing Zhang, Si Lei, Shang-Jie Wu
BACKGROUND: Hypoxic pulmonary arterial hypertension (PAH) is a crippling disease with limited therapeutic methods. The imbalance of T helper 17 cell (Th17)/regulatory T cell (Treg) plays an important role in the development of Hypoxic PAH. However, whether targeting the ras homolog family member A-Rho kinase (RhoA-ROCK) pathway (activation and inhibition) by lysophosphatidic acid (LPA) and fasudil (FSD) regulate T-cell homeostasis in Hypoxic PAH remain unknown. OBJECTIVE: To examine the effects of LPA and FSD on hypoxic pulmonary vascular remodeling and homeostasis of Th17/Treg cells in Hypoxic PAH...
April 16, 2018: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/29673861/il-6-knockout-mice-are-protected-from-cocaine-induced-kindling-behaviors-possible-involvement-of-jak2-stat3-and-pacap-signalings
#12
Huynh Nhu Mai, Yoon Hee Chung, Eun-Joo Shin, Naveen Sharma, Ji Hoon Jeong, Choon-Gon Jang, Kuniaki Saito, Toshitaka Nabeshima, Dora Reglodi, Hyoung-Chun Kim
IL-6 has been recognized as an anticonvulsant against certain neuroexcitotoxicities. We aimed to investigate on the interactive role between IL-6 and PACAP in cocaine-induced kindling behaviors. Although we found that cocaine (45 mg/kg, i.p./day x 5) significantly increased IL-6 and TNF-α expression, it resulted in a decrease in IFN-γ expression. We observed that the cocaine-induced increase in IL-6 expression was more pronounced than that in TNF-α expression. Genetic depletion of IL-6 significantly activated cocaine kindling behaviors...
April 16, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29673456/-intracerebroventricular-injection-of-mir-7-inhibits-secondary-brain-injury-induced-by-intracerebral-hemorrhage-via-egfr-stat3-pathway-in-rats
#13
Hong Qian, Ke Hu, Ming Xie, Heng Wu, Wenjun Li, Bin Wu, Rongyong Man, Meng Nie
Objective To observe the effects of microRNA-7 (miR-7) on intracerebral hemorrhage-induced brain injury in rats and explore the underlying mechanism. Methods Seventy-five SD rats were used to establish intracerebral hemorrhage model through injection of VII collagenase into the pallidum. These rats were then divided into control, miR-7 agomir, agomir control, miR-7 antagomir and antagomir control groups, containing 15 animals in each group. On day 2 after modeling, rats were injected with 10 μL of physiological saline, miR-7 agomir, agomir control, miR-7 antagomir and antagomir control via the lateral ventricle, respectively...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29673453/-knockdown-of-stat3-inhibits-proliferation-and-migration-of-hepg2-hepatoma-cells-induced-by-ifn1
#14
Xiaofang Li, Yuqi Wang, Ben Yan, Peipei Fang, Chao Ma, Ning Xu, Xiaoyan Fu, Shujuan Liang
Objective To prepare lentiviruses expressing shRNA sequences targeting human signal transducer and activator of transcription 3 (STAT3) and detect the effect of STAT3 knockdown on type I interferon (IFN1)-induced proliferation and migration in HepG2 cells. Methods Four STAT3-targeting shRNA sequences (shRNA1-shRNA4) and one control sequence (Ctrl shRNA) were selected and cloned respectively into pLKO.1-sp6-pgk-GFP to construct shRNA-expressing vectors. Along with backbone psPAX2 and pMD2.G vectors, they were separately transfected into HEK293T cells to prepare lentiviruses...
February 2018: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/29672888/a-novel-fibroblast-growth-factor-15-dependent-and-bile-acid-independent-promotion-of-liver-regeneration-in-mice
#15
Bo Kong, Runbin Sun, Mingxing Huang, Monica D Chow, Xiao-Bo Zhong, Wen Xie, Yi-Horng Lee, Grace L Guo
The role of intestine-derived factors in promoting liver regeneration after partial hepatectomy (PHx) are not entirely known, but bile acids (BAs) and fibroblast growth factor 15 (Fgf15) that is highly expressed in the mouse ileum could promote hepatocyte proliferation. Fgf15 strongly suppresses the synthesis of BAs and emerging evidence indicates that Fgf15 is important for liver regeneration. The mechanisms by which Fgf15 promotes liver regeneration are unclear, but Fgf15 may do so indirectly by reducing BA levels and/or directly by promoting cell proliferation...
April 19, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/29670920/methotrexate-combined-with-4-hydroperoxycyclophosphamide-downregulates-multidrug-resistance-p-glycoprotein-expression-induced-by-methotrexate-in-rheumatoid-arthritis-fibroblast-like-synoviocytes-via-the-jak2-stat3-pathway
#16
Kaili Qin, Kailin Chen, Wenpeng Zhao, Xiangcong Zhao, Jing Luo, Qun Wang, Chong Gao, Xiaofeng Li, Caihong Wang
Objective: Rheumatoid arthritis (RA) multidrug resistance is associated with P-glycoprotein (P-gp) overexpression. We investigated the effects of methotrexate (MTX) alone and combined with 4-hydroperoxycyclophosphamide (4-HC) on P-gp expression in fibroblast-like synoviocytes (FLSs) from patients with RA and examined the signaling pathway involved. Methods: RA-FLSs were treated with MTX, MTX + 4-HC, AG490 + MTX, or AG490 + MTX + 4-HC for 72 h. Proliferation inhibition rates were determined by MTT assay; P-gp expression was measured by flow cytometry and real-time polymerase chain reaction (RT-PCR); JAK2 and STAT3 were measured by RT-PCR and cell-based ELISA to assess STAT3 signaling...
2018: Journal of Immunology Research
https://www.readbyqxmd.com/read/29670389/fgf23-modulates-the-effects-of-erythropoietin-on-gene-expression-in-renal-epithelial-cells
#17
Mitsuru Yashiro, Masaki Ohya, Toru Mima, Yumi Ueda, Yuri Nakashima, Kazuki Kawakami, Yohei Ishizawa, Shuto Yamamoto, Sou Kobayashi, Takurou Yano, Yusuke Tanaka, Kouji Okuda, Tomohiro Sonou, Tomohiro Shoshihara, Yuko Iwashita, Yu Iwashita, Kouichi Tatsuta, Ryo Matoba, Shigeo Negi, Takashi Shigematsu
Background: FGF23 plays an important role in calcium-phosphorus metabolism. Other roles of FGF23 have recently been reported, such as commitment to myocardium enlargement and immunological roles in the spleen. In this study, we aimed to identify the roles of FGF23 in the kidneys other than calcium-phosphorus metabolism. Methods: DNA microarrays and bioinformatics tools were used to analyze gene expression in mIMCD3 mouse renal tubule cells following treatment with FGF23, erythropoietin and/or an inhibitor of ERK...
2018: International Journal of Nephrology and Renovascular Disease
https://www.readbyqxmd.com/read/29669962/renal-protective-effect-of-paeoniflorin-by-inhibition-of-jak2-stat3-signaling-pathway-in-diabetic-mice
#18
Xinyu Li, Yan Wang, Kun Wang, Yonggui Wu
Paeoniflorin is the main bioactive components of the root of P.lactiflora Pall., and has been widely used as an anti-inflammation and immunomodulatory agent. However, the effect and mechanisms of Paeoniflorin in diabetic nephropathy (DN) remains to be elucidated. In the present study, streptozotocin (STZ)-induced type 1 diabetic mice model was used to investigate the protective effect of Paeoniflorin and the role of the Janus kinase (JAK) 2/signal transducer (STAT) 3 signaling pathway on DN. After treatment with Paeoniflorin at a dose of 25, 50 and 100 mg/kg once a day for 12 weeks, both the functional and histological damage to diabetic mice kidney had been attenuated significantly...
April 19, 2018: Bioscience Trends
https://www.readbyqxmd.com/read/29669350/periplaneta-americana-extract-may-attenuate-renal-fibrosis-through-inhibiting-janus-tyrosine-kinase-2-signal-transducer-and-activator-of-transcription-3-pathway
#19
Jingsong Liu, Lin Zhou, Liyu He, Ying Zhong, Xiaobai Zhang, Bofei Xiao, Guoyong Liu
BACKGROUND: Periplaneta americana is one of the ancient insect groups with the strongest vitality. Periplaneta americana extract (PAE) has been explored as an alternative remedy for many diseases. Although much progress has been made in the study about PAE, the role of the drug in renal disease is rarely reported, especially in renal fibrosis. This study was designed to evaluate the renoprotective effect of PAE treatment to renal fibrosis. METHOD: An in vivo, unilateral ureteral obstruction (UUO) mouse model was built...
April 17, 2018: Pharmacology
https://www.readbyqxmd.com/read/29669207/ros-inducing-micelles-sensitize-tumor-associated-macrophages-to-tlr3-stimulation-for-potent-immunotherapy
#20
Lanlan Liu, Huamei He, Ruijing Liang, Huqiang Yi, Xiaoqing Meng, Zhikuan Chen, Hong Pan, Yifan Ma, Lintao Cai
One approach to cancer immunotherapy is the repolarization of immunosuppressive tumor-associated macrophages (TAMs) to anti-tumor M1 macrophages. The present study developed galactose-functionalized zinc protoporphyrin IX (ZnPP) grafted poly(L-lysine)-b-poly(ethyleneglycol) polypeptide micelles (ZnPP PM ) for TAM-targeted immunopotentiator delivery, which aimed at in vivo repolarization of TAMs to anti-tumor M1 macrophages. The outcomes revealed that ROS-inducing ZnPP PM demonstrated specificity for the in vitro and in vivo targeting of macrophages, elevated the level of ROS, and lowered STAT3 expression in BM-TAMs...
April 18, 2018: Biomacromolecules
keyword
keyword
14617
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"