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https://www.readbyqxmd.com/read/29352487/disaccharide-combinations-and-the-expression-of-enolase3-and-plasma-membrane-ca2-atpase-isoform-in-sturgeon-sperm-cryopreservation
#1
M D Xi, P Li, H Du, X M Qiao, Z G Liu, Q W Wei
Acipenser sinensis and Acipenser dabryanus are critically endangered species, so germplasm conservation via cryopreservation of sperm is necessary. Disaccharides can act as membrane-impermeable cryoprotectants, and enolase3 (ENO3) and plasma membrane Ca2+ ATPase isoform (PMCA2) are proteins associated with sperm quality. We considered seven characteristics of sperm quality in cultured brood stock from A. sinensis and A. dabryanus. We tested use of sucrose or trehalose alone and in combination at different concentrations for cryopreservation of A...
January 19, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29351899/web-based-survey-application-to-collect-contextually-relevant-geographic-data-with-exposure-times-application-development-and-feasibility-testing
#2
Abby Rudolph, Karin Tobin, Jonathan Rudolph, Carl Latkin
BACKGROUND: Although studies that characterize the risk environment by linking contextual factors with individual-level data have advanced infectious disease and substance use research, there are opportunities to refine how we define relevant neighborhood exposures; this can in turn reduce the potential for exposure misclassification. For example, for those who do not inject at home, injection risk behaviors may be more influenced by the environment where they inject than where they live...
January 19, 2018: JMIR Public Health and Surveillance
https://www.readbyqxmd.com/read/29351878/kinetic-studies-of-the-aop-radical-based-oxidative-and-reductive-destruction-of-pesticides-and-model-compounds-in-water
#3
Kristin K Clark, Stephen P Mezyk, Amberashley Abbott, James J Kiddle
Absolute second-order rate constants for hydroxyl radical (HO) reaction with four organophosphorus pesticides, malathion, parathion, fenthion and ethion, and a suite of model compounds of structure (EtO)2P(S)-X (where X = Cl, F, SH, SEt, OCH2CF3, OEt, NH2, and CH3) were measured using electron pulse radiolysis and transient absorption techniques. Specific values were determined for these four pesticides as k = (3.89 ± 0.28) x 109, (2.20 ± 0.15) x 109, (2.02 ± 0.15) x 109 and (2.93 ± 0...
December 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/29351864/prolonging-the-response-movement-inhibits-the-feed-forward-motor-program-in-the-sustained-attention-to-response-task
#4
Kyle M Wilson, Neil R de Joux, Kristin M Finkbeiner, Paul N Russell, Jenny R Retzler, William S Helton
Despite widespread use in clinical and experimental contexts, debate continues over whether or not the Sustained Attention to Response Task (SART) successfully measures sustained attention. Altering physical aspects of the response movement required to SART stimuli may help identify whether performance is a better measure of perceptual decoupling, or response strategies and motor inhibition. Participants completed a SART where they had to manually move a mouse cursor to respond to stimuli, and another SART where this extra movement was not required, as in a typical SART...
January 16, 2018: Acta Psychologica
https://www.readbyqxmd.com/read/29351668/evaluation-of-the-rapid-carbapenem-inactivation-method-rcim-a-phenotypic-screening-test-for-carbapenemase-producing-enterobacteriaceae
#5
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29351574/self-reported-knowledge-correct-knowledge-and-use-of-uk-drinking-guidelines-among-a-representative-sample-of-the-english-population
#6
Penny Buykx, Jessica Li, Lucy Gavens, Lucie Hooper, Elena Gomes de Matos, John Holmes
Aims: Promotion of lower risk drinking guidelines is a commonly used public health intervention with various purposes, including communicating alcohol consumption risks, informing drinkers' decision-making and, potentially, changing behaviour. UK drinking guidelines were revised in 2016. To inform potential promotion of the new guidelines, we aimed to examine public knowledge and use of the previous drinking guidelines, including by population subgroup. Methods: A demographically representative, cross-sectional online survey of 2100 adults living in England in July 2015 (i...
January 17, 2018: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
https://www.readbyqxmd.com/read/29350689/safety-of-intravitreal-injection-of-ranibizumab-in-early-pregnancy-a-series-of-three-cases
#7
P Fossum, C Couret, B Briend, M Weber, L Lagarce
PurposeRanibizumab is used in the treatment of choroidal neovascularization (CNV). Although systemic exposure to ranibizumab is low after ocular administration, its mechanism of action must be regarded as potentially teratogenic and embryo-fetotoxic. Women are advised to wait 3 months after the last dose of treatment with ranibizumab before conceiving. Little is known about the fetal side-effects of this drug.MethodsThree pregnant women were treated with ranibizumab. One patient had idiopathic CNV.ResultsAfter receiving injections at 10 and 21 weeks after her last menstrual period (LMP), she gave birth to a healthy child...
January 19, 2018: Eye
https://www.readbyqxmd.com/read/29349777/in-bed-cycling-in-the-icu-patient-safety-and-recollections-with-motivational-effects
#8
M Ringdal, M Warren Stomberg, K Egnell, E Wennberg, R Zätterman, C Rylander
BACKGROUND: In-bed cycling (IBC) is gaining interest for implementation in intensive care units. Our main objective was to explore patient recollections and experiences of early mobilization, including IBC. Secondly, we aimed to examine if IBC was safe and feasible. METHODS: Eleven participants were interviewed about their experiences during their critical illnesses and active mobilization in the intensive care unit. The interviews were analyzed thematically. Six participants were also monitored for physiological reactions and adverse events during IBC while mechanically ventilated...
January 18, 2018: Acta Anaesthesiologica Scandinavica
https://www.readbyqxmd.com/read/29349773/progestin-only-and-combined-oral-contraceptives-and-receptor-defined-premenopausal-breast-cancer-risk-the-norwegian-women-and-cancer-study
#9
Marit Busund, Nora S Bugge, Tonje Braaten, Marit Waaseth, Charlotta Rylander, Eiliv Lund
Receptor-defined subtypes of breast cancer represent distinct cancer types and have differences in risk factors. Whether the two main hormonal forms of oral contraceptives (OCs); i.e. progestin-only (POC) and combined oral contraceptives (COC), are differentially associated with these subtypes are not well known. The aim of this study was to assess the effect of POC and COC use on hormone receptor-defined breast cancer risk in premenopausal women in a prospective population-based cohort - The Norwegian Women and Cancer study (NOWAC)...
January 19, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29348673/quantification-of-massively-parallel-sequencing-libraries-a-comparative-study-of-eight-methods
#10
Christian Hussing, Marie-Louise Kampmann, Helle Smidt Mogensen, Claus Børsting, Niels Morling
Quantification of massively parallel sequencing libraries is important for acquisition of monoclonal beads or clusters prior to clonal amplification and to avoid large variations in library coverage when multiple samples are included in one sequencing analysis. No gold standard for quantification of libraries exists. We assessed eight methods of quantification of libraries by quantifying 54 amplicon, six capture, and six shotgun fragment libraries. Chemically synthesized double-stranded DNA was also quantified...
January 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29347917/does-different-information-disclosure-on-placebo-control-affect-blinding-and-trial-outcomes-a-case-study-of-participant-information-leaflets-of-randomized-placebo-controlled-trials-of-acupuncture
#11
Soyeon Cheon, Hi-Joon Park, Younbyoung Chae, Hyangsook Lee
BACKGROUND: While full disclosure of information on placebo control in participant information leaflets (PILs) in a clinical trial is ethically required during informed consent, there have been concerning voices such complete disclosures may increase unnecessary nocebo responses, breach double-blind designs, and/or affect direction of trial outcomes. Taking an example of acupuncture studies, we aimed to examine what participants are told about placebo controls in randomized, placebo-controlled trials, and how it may affect blinding and trial outcomes...
January 18, 2018: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/29346721/emulsion-electrospinning-of-polytetrafluoroethylene-ptfe-nanofibrous-membranes-for-high-performance-triboelectric-nanogenerators
#12
Pengfei Zhao, Navneet Soin, K Prashanthi, Jinkai Chen, Shurong Dong, Erping Zhou, Zhigang Zhu, Anand Arcot Narasimulu, Carlo D Montemagno, Liyang Yu, Jikui Luo
Electrospinning is a simple, versatile technique for fabricating fibrous nanomaterials with the desirable features of extremely high porosities and large surface areas. Using emulsion electrospinning, polytetrafluoroethylene/polyethene oxide (PTFE/PEO) membranes were fabricated followed by a sintering process to obtain pure PTFE fibrous membranes, which were further utilised against a polyamide 6 (PA6) membrane for vertical contact-mode triboelectric nanogenerators (TENGs). Electrostatic force microscopy (EFM) measurements of the sintered electrospun PTFE membranes revealed the presence of both positive and negative surface charges owing to the transfer of positive charge from PEO which further corroborated by FTIR measurements...
January 18, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29345993/socioeconomic-outcomes-of-women-who-receive-and-women-who-are-denied-wanted-abortions-in-the-united-states
#13
Diana Greene Foster, M Antonia Biggs, Lauren Ralph, Caitlin Gerdts, Sarah Roberts, M Maria Glymour
OBJECTIVES: To determine the socioeconomic consequences of receipt versus denial of abortion. METHODS: Women who presented for abortion just before or after the gestational age limit of 30 abortion facilities across the United States between 2008 and 2010 were recruited and followed for 5 years via semiannual telephone interviews. Using mixed effects models, we evaluated socioeconomic outcomes for 813 women by receipt or denial of abortion care. RESULTS: In analyses that adjusted for the few baseline differences, women denied abortions who gave birth had higher odds of poverty 6 months after denial (adjusted odds ratio [AOR] = 3...
January 18, 2018: American Journal of Public Health
https://www.readbyqxmd.com/read/29345967/reclassifying-acute-respiratory-distress-syndrome
#14
Giorgia Maiolo, Francesca Collino, Francesco Vasques, Francesca Rapetti, Tommaso Tonetti, Federica Romitti, Massimo Cressoni, Davide Chiumello, Onnen Mörer, Peter Herrmann, Tim Friede, Michael Quintel, Luciano Gattinoni
RATIONALE: The ratio of PaO2 to FiO2 (P/F) defines ARDS severity and suggests appropriate therapies. OBJECTIVES: We investigated 1) whether a 150 mmHg P/F threshold within the range of moderate ARDS (100-200 mmHg) would define two, more homogeneous subgroups, 2) which criteria led the clinicians to apply ECMO in severe ARDS. Methods & Measurements: At 150 mmHg P/F threshold, moderate patients were split in mild-moderate (n=50) and moderate-severe (n=55). Severe ARDS patients (FiO2 not available in 3 patients ) were split in higher (n=63) and lower-FiO2 (n=18) at 80% FiO2 threshold...
January 18, 2018: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/29345843/does-the-use-of-epiduroscopic-lysis-of-adhesions-reduce-the-need-for-spinal-cord-stimulation-in-failed-back-surgery-syndrome-a-short-term-pilot-study
#15
S J Tuijp, J Van Zundert, P De Vooght, M Puylaert, R Mestrum, R Heylen, P Vanelderen
BACKGROUND AND AIMS: Persistent low back pain after initially successful surgery that is not attributed to structural deficits, is called failed back surgery syndrome (FBSS). When conservative and minimal invasive therapy fail, the recommended treatment is spinal cord stimulation (SCS). Because epidural fibrosis can be a contributing factor in the majority of FBSS patients, lumbosacral epiduroscopic lysis of adhesions may be considered as a less invasive alternative treatment option. We hypothesized that the use of epiduroscopic lysis of adhesions could reduce the need for SCS...
January 18, 2018: Pain Practice: the Official Journal of World Institute of Pain
https://www.readbyqxmd.com/read/29345800/haplotype-based-genotyping-by-sequencing-in-oat-genome-research
#16
Wubishet A Bekele, Charlene P Wight, Shiaoman Chao, Catherine J Howarth, Nicholas A Tinker
In a de-novo genotyping-by-sequencing (GBS) analysis of short, 64-base tag-level haplotypes in 4657 accessions of cultivated oat, we discovered 164741 tag-level (TL) genetic variants containing 241224 SNPs. From this, the marker-density of an oat consensus map was increased by the addition of more than 70000 loci. The mapped TL genotypes of a 635-line diversity panel were used to infer chromosome-level (CL) haplotype maps. These maps revealed differences in the number and size of haplotype blocks, as well as differences in haplotype diversity between chromosomes and subsets of the diversity panel...
January 18, 2018: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/29345492/cigdem-kagitcibasi-1940-2017
#17
Zeynep Aycan, Zeynep Cemalcilar
Presents an obituary for Cigdem Kagitcibasi. Kagitcibasi is considered one of the founders of cross-cultural psychology. Her theoretical and applied research focused on human development in socio-cultural context, leading her to question the applicability of mainstream psychology to the majority (non-Western) world. Her emphasis on culture, social change, and the family as important aspects of the context of human development gave birth to Family Change Theory and the Theory of Autonomous-Related Self. Kagitcibasi was the first female president of the International Association for Cross-Cultural Psychology, the vice president of the International Union of Psychological Science and the International Social Science Council...
January 2018: American Psychologist
https://www.readbyqxmd.com/read/29345392/who-may-benefit-from-on-demand-control-of-deep-brain-stimulation-noninvasive-evaluation-of-parkinson-patients
#18
Daniel Graupe, Nivedita Khobragade, Daniela Tuninetti, Ishita Basu, Konstantin V Slavin, Leo Verhagen Metman
BACKGROUND: In closed-loop on-demand control (ODC) of deep brain stimulation (DBS), stimulation is applied only when symptoms appear. Following stimulation of a fixed duration, DBS is switched off until the symptoms reappear. By repeating these demand-driven cycles, the amount of stimulation delivered can be decreased, thereby reducing DBS side-effects and improving battery-life of the pulse-generator. This article introduces Ro metric for quantification of degree of benefit of ODC and explores candidate selection in tremor-dominant Parkinson's disease (PD)...
January 18, 2018: Neuromodulation: Journal of the International Neuromodulation Society
https://www.readbyqxmd.com/read/29345081/screening-for-latent-tuberculosis-infection-among-patients-with-rheumatoid-arthritis-in-the-era-of-biologics-and-targeted-synthetic-disease-modifying-anti-rheumatic-drugs-in-india-a-high-burden-tb-country-the-importance-of-mantoux-and-quantiferon-tb-gold-tests
#19
Anand N Malaviya, Vishal K Aggarwal, Roopa Rawat, Sadhana Baghel, Ravita Thakran, Qamar Zaheer, Shriram Garg, Sanjiv Kapoor
AIM: To test the validity of an augmented tuberculosis skin test (a-TST) combined with Quantiferon TB-gold® (QFTG) test for the screening of latent tuberculosis infection (LTBI) in patients with rheumatoid arthritis (RA) being considered for treatment with biologic disease-modifying anti-rheumatic drugs or targeted synthetic disease-modifying anti-rheumatic drugs. METHOD: Standard TST using 1 tuberculin unit (TU) of purified protein derivative (PPD, RT23 strain) was carried out...
January 17, 2018: International Journal of Rheumatic Diseases
https://www.readbyqxmd.com/read/29344776/dawning-of-a-new-era-in-trp-channel-structural-biology-by-cryo-electron-microscopy
#20
REVIEW
M Gregor Madej, Christine M Ziegler
Cryo-electron microscopy (cryo-EM) permits the determination of atomic protein structures by averaging large numbers of individual projection images recorded at cryogenic temperatures-a method termed single-particle analysis. The cryo-preservation traps proteins within a thin glass-like ice layer, making literally a freeze image of proteins in solution. Projections of randomly adopted orientations are merged to reconstruct a 3D density map. While atomic resolution for highly symmetric viruses was achieved already in 2009, the development of new sensitive and fast electron detectors has enabled cryo-EM for smaller and asymmetrical proteins including fragile membrane proteins...
January 17, 2018: Pflügers Archiv: European Journal of Physiology
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