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https://www.readbyqxmd.com/read/28696069/hcc-associated-single-nucleotide-variants-and-deletions-identified-using-genome-wide-high-throughput-analysis-of-hbv
#1
Wen-Chun Liu, I-Chin Wu, Yen-Chien Lee, Chih-Peng Lin, Ji-Hong Cheng, Yih-Jyh Lin, Chia-Jui Yen, Pin-Nan Cheng, Pei-Fu Li, Yi-Ting Cheng, Pei-Wen Cheng, Koun-Tem Sun, Shu-Ling Yan, Jia-Jhen Lin, Jui-Chu Yang, Kung-Chao Chang, Cheng-Hsun Ho, Vincent S Tseng, Bill Chia-Han Chang, Jaw-Ching Wu, Ting-Tsung Chang
This study investigated hepatitis B virus (HBV) single nucleotide variants (SNVs) and deletion mutations linked with hepatocellular carcinoma (HCC). Ninety-three HCC patients and 108 non-HCC patients were enrolled for HBV genome-wide next-generation sequencing (NGS) analysis. Systemic literature review and meta-analysis were performed to validate NGS-defined HCC-associated SNVs and deletions. The experimental results identified 60 NGS-defined HCC-associated SNVs, including 41 novel SNVs, and their pathogenic frequencies...
July 11, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28673869/impact-of-deletions-and-mutations-in-hepatitis-b-virus-envelope-proteins-on-serological-profile-and-clinical-evolution
#2
Brice Malve, Marine Eschlimann, Shaunagh Galgey, Honorine Fenaux, Fabien Zoulim, François Goehringer, Christian Rabaud, Thierry May, Hélène Jeulin, Evelyne Schvoerer
The Hepatitis B virus (HBV) envelope glycoproteins are essential for viral entry into the hepatocyte and are also targets for host immune response. The study of these proteins could allow us to highlight molecular hot points influencing HBV fitness, which would subsequently modify the clinical evolution of the disease, both under anti-viral therapy or without treatment. The present short communication underlines the importance of the high variability in HBV envelope proteins, in regard with the literature and in our hands, for HBV-infected patients either on anti-HBV treatment or not...
July 1, 2017: Virus Research
https://www.readbyqxmd.com/read/28513915/impact-of-a-determinant-mutations-on-detection-of-hepatitis-b-surface-antigen-hbsag-in-hbv-strains-from-chinese-patients-with-occult-hepatitis-b
#3
Xiangyan Huang, Chenyun Ma, Qiang Zhang, Qingfen Shi, Tao Huang, Chao Liu, Jie Li, F Blaine Hollinger
This study was designed to detect mutations that occur within the "a" determinant in the S gene of the hepatitis B virus (HBV) in patients with occult hepatitis B (OHB), and to analyze the influence of these mutations on expression and reactivity of the hepatitis B surface antigen (HBsAg). Twenty-three certified OHB samples were compared to 32 HBsAg positive samples from patients with chronic hepatitis B. The median HBV DNA levels in the OHB group were significantly lower than those in the control group (P < 0...
May 17, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28456864/a-randomized-controlled-clinical-trial-to-evaluate-the-immunogenicity-of-a-pres-s-hepatitis-b-vaccine-sci-b-vac%C3%A2-as-compared-to-engerix-b-%C3%A2-among-vaccine-na%C3%A3-ve-and-vaccine-non-responder-dialysis-patients
#4
E Elhanan, M Boaz, I Schwartz, D Schwartz, G Chernin, H Soetendorp, A Gal Oz, A Agbaria, T Weinstein
BACKGROUND: Dialysis patients have a suboptimal response to hepatitis B (HBV) vaccination. This study aimed to compare the immunogenicity of two vaccines: the third-generation Sci-B-Vac™ vs. the second-generation Engerix B(®). The cohort included two groups of dialysis patients: naïve and previously vaccinated non-responders. Primary endpoints were antibody titers ≥10 IU/L at 3 and 7 month post-vaccination. Secondary objectives were seroprotection rates in vaccine-naïve patients and in previously vaccinated non-responders...
April 29, 2017: Clinical and Experimental Nephrology
https://www.readbyqxmd.com/read/28414318/initial-sites-of-hepadnavirus-integration-into-host-genome-in-human-hepatocytes-and-in-the-woodchuck-model-of-hepatitis-b-associated-hepatocellular-carcinoma
#5
R Chauhan, N D Churchill, P M Mulrooney-Cousins, T I Michalak
Hepatitis B virus (HBV) and the closely related woodchuck hepatitis virus (WHV) are potent carcinogens that trigger development of primary hepatocellular carcinoma (HCC). The initial sites of hepadnavirus-host genome integration, their diversity and kinetics of formation can be central to virus persistence and the initiation and progression of HCC. To recognize the nature of the very early virus-host interactions, we explored de novo infection of human hepatocyte-like HepaRG cells with authentic HBV and naive woodchucks with WHV...
April 17, 2017: Oncogenesis
https://www.readbyqxmd.com/read/28373061/sequence-analysis-and-functional-characterization-of-full-length-hepatitis-b-virus-genomes-from-korean-cirrhotic-patients-with-or-without-liver-cancer
#6
Huailiang Zhou, Dina Gewaily, Sang Hoon Ahn, Carina Preskill, Yongxiang Wang, Li Zong, Jing Zhang, Kwang-Hyub Han, Jack Wands, Jisu Li, Shuping Tong
This study aimed to identify and characterize mutations in the hepatitis B virus (HBV) genome associated with advanced liver diseases. The 3.2-kb HBV genome of the C2 subgenotype was amplified from sera of 18 cirrhotic Korean patients with (10) or without (8) hepatocellular carcinoma (HCC), and two clones per patient were characterized by transient transfection experiments in human hepatoma cells. While A1762T/G1764A core promoter mutations were highly prevalent in both groups, the G1896A precore mutation to abolish hepatitis B e antigen (HBeAg) expression was more common in HCC clones (55% vs...
April 1, 2017: Virus Research
https://www.readbyqxmd.com/read/28236303/characterization-of-hepatitis-b-virus-hbv-pres-s-gene-mutations-in-blood-donors-with-occult-hbv-infection-in-the-baoji-area-of-north-china
#7
Hao Liao, Yan Liu, Jianhong Chen, Weiping Ding, Xiaodong Li, Zhihui Xu, Yuan Yang, Rongjuan Chen, Lanlan Si, Xiaoyuan Xu, Jianxun Guo, Dongping Xu
BACKGROUND: Occult hepatitis B virus (HBV) infection (OBI) in blood donors was investigated in the Baoji area of North China, and OBI-related viral mutations in donors were characterized. STUDY DESIGN AND METHODS: In total, 110,843 blood donor samples that were consecutively collected from December 2011 to March 2015 at the Baoji Blood Center were examined. Hepatitis B surface antigen-negative and HBV DNA-positive OBI samples were amplified for sequence analysis of OBI-related mutations in the HBV preS/S region...
March 2017: Transfusion
https://www.readbyqxmd.com/read/27930595/hepatitis-b-viremia-in-completely-immunized-individuals-negative-for-anti-hepatitis-b-core-antibody
#8
Ming-Wei Lai, Tzou-Yien Lin, Kung-Hao Liang, Wey-Ran Lin, Chau-Ting Yeh
The presence of anti-hepatitis B virus (HBV) core antibody (anti-HBc) is considered a sensitive lifetime marker of HBV infection. Here, we examined this dogma by investigating the prevalence of hepatitis B viremia in anti-HBc negative complete vaccines in Taiwan.A total of 795 participants (1.7-20.0 years old) had completed HBV vaccination in infancy and were anti-HBc negative. Serum samples were available for 460 individuals with isolated anti-HBV surface antibodies (anti-HBs) (HBsAg-negative and anti-HBc negative) and for 245 individuals who tested negative for all 3 markers (triple seronegative)...
December 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/27720823/higher-prevalence-of-cancer-related-mutations-1762t-1764a-and-pres-deletions-in-hepatitis-b-virus-hbv-isolated-from-hbv-hiv-co-infected-compared-to-hbv-mono-infected-chinese-adults
#9
Kai-Wen Li, Anna Kramvis, Shujia Liang, Xiang He, Qin-Yan Chen, Chao Wang, Qing-Li Yang, Li-Ping Hu, Hui-Hua Jia, Zhong-Liao Fang
In the era of combination therapy for human immunodeficiency virus (HIV), liver disease including hepatocellular carcinoma (HCC), are the major causes of death for patients co-infected with hepatitis B virus (HBV) and HIV. However, the mechanisms remain obscure. We aimed to determine whether HCC-related HBV mutations including 1762T/1764A double mutation and pre-S deletions occur more frequently in HBV/HIV co-infected individuals compared to HBV mono-infected individuals. In this study, the basic core promoter (BCP) and the preS/S regions of HBV isolated from 61 pairs of HBV/HIV co-infected and HBV mono-infected participants were analyzed...
January 2, 2017: Virus Research
https://www.readbyqxmd.com/read/27568223/immunotherapy-with-the-pres-based-grass-pollen-allergy-vaccine-bm32-induces-antibody-responses-protecting-against-hepatitis-b-infection
#10
Carolin Cornelius, Katrin Schöneweis, Fanny Georgi, Milena Weber, Verena Niederberger, Petra Zieglmayer, Katarzyna Niespodziana, Michael Trauner, Harald Hofer, Stephan Urban, Rudolf Valenta
BACKGROUND: We have constructed and clinically evaluated a hypoallergenic vaccine for grass pollen allergy, BM32, which is based on fusion proteins consisting of peptides from the IgE binding sites of the major grass pollen allergens fused to preS (preS1+preS2), a domain of the hepatitis B virus (HBV) large envelope protein which mediates the viral attachment and entry. Aim of this study was the characterization of the HBV-specific immune response induced by vaccination of allergic patients with BM32 and the investigation of the vaccines' potential to protect against infection with HBV...
September 2016: EBioMedicine
https://www.readbyqxmd.com/read/27458715/molecular-epidemiology-of-co-infection-with-hepatitis-b-virus-and-human-immunodeficiency-virus-hiv-among-adult-patients-in-harare-zimbabwe
#11
Ian Baudi, Sayuki Iijima, Nyasha Chin'ombe, Sekesai Mtapuri-Zinyowera, Shuko Murakami, Masanori Isogawa, Atsuko Hachiya, Yasumasa Iwatani, Yasuhito Tanaka
The objective of this study was to determine the prevalence of co-infection with hepatitis B virus (HBV) and human immunodeficiency virus (HIV) and the genetic characteristics of both viruses among pre-HIV-treatment patients in Harare, Zimbabwe. This cross-sectional survey involved 176 remnant plasma samples collected from consenting HIV patients (median age 35 [18-74]) between June and September 2014. HBV seromarkers were determined by high-sensitivity chemiluminescence assays. Molecular evolutionary analyses were conducted on the basal core promoter/precore (BCP/PC) and S regions of HBV, as well as part of the HIV pol region...
February 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/27415434/pres-deletion-profiles-of-hepatitis-b-virus-hbv-are-associated-with-clinical-presentations-of-chronic-hbv-infection
#12
Xiaodong Li, Yaqun Qin, Yan Liu, Fan Li, Hao Liao, Shanshan Lu, Yan Qiao, Dongping Xu, Jin Li
BACKGROUND: The association of hepatitis B virus (HBV) preS1 and preS2 deletions with progressive liver diseases are not fully understood. OBJECTIVE: The study aimed to investigate characteristics of HBV preS deletion in HBV-infected patients with different illness categories. STUDY DESIGN: Total of 539 HBV-infected patients were enrolled in the study, including 146 with chronic hepatitis B (CHB), 111 with HBV-related liver cirrhosis (LC), 146 with HBV-related acute-on-chronic liver failure (ACLF), and 136 with HBV-related hepatocellular carcinoma (HCC)...
September 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27345250/characterization-of-the-3rd-international-standard-for-hepatitis-b-virus-surface-antigen-hbsag
#13
Pia L Seiz, Christina Mohr, Dianna E Wilkinson, John Ziebuhr, Christian G Schüttler, Wolfram H Gerlich, Dieter Glebe
BACKGROUND: HBsAg is the most important marker for laboratory diagnosis of HBV infection. Validation and quality control of HBsAg tests requires International Standards (IS). Recently the 2nd IS was replaced by the 3rd IS. Both IS are made from plasma-derived hepatitis B vaccines, but production and geographical origin are different. OBJECTIVE: Characterization of the HBsAg in the source material (SM) for the 3rd IS and comparison with the 2nd IS and native HBsAg...
September 2016: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/27321908/a-slow-maturation-process-renders-hepatitis-b-virus-infectious
#14
Stefan Seitz, Caroline Iancu, Tassilo Volz, Walter Mier, Maura Dandri, Stephan Urban, Ralf Bartenschlager
Hepatitis B virus (HBV) replication is strictly limited to the liver. Virions attach to hepatocytes through interactions of the viral PreS envelope protein domain with heparan sulfate proteoglycans (HSPGs). However, HSPG is ubiquitously present on many cell types, suggesting that HBV employs mechanisms to avoid attachment at extrahepatic sites. We demonstrate that HBV particles are released from cells in an inactive form with PreS hidden in the interior. These HSPG-non-binding (N-type) particles develop receptor binding competence by translocating PreS across the envelope onto their surface...
July 13, 2016: Cell Host & Microbe
https://www.readbyqxmd.com/read/27252524/single-molecule-sequencing-reveals-complex-genome-variation-of-hepatitis-b-virus-during-15-years-of-chronic-infection-following-liver-transplantation
#15
B D Betz-Stablein, A Töpfer, M Littlejohn, L Yuen, D Colledge, V Sozzi, P Angus, A Thompson, P Revill, N Beerenwinkel, N Warner, F Luciani
UNLABELLED: Chronic hepatitis B (CHB) is prevalent worldwide. The infectious agent, hepatitis B virus (HBV), replicates via an RNA intermediate and is error prone, leading to the rapid generation of closely related but not identical viral variants, including those that can escape host immune responses and antiviral treatments. The complexity of CHB can be further enhanced by the presence of HBV variants with large deletions in the genome generated via splicing (spHBV variants). Although spHBV variants are incapable of autonomous replication, their replication is rescued by wild-type HBV...
August 15, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27182775/characterization-of-novel-hepatitis-b-virus-pres-s-gene-mutations-in-a-patient-with-occult-hepatitis-b-virus-infection
#16
Jianhong Chen, Yan Liu, Jun Zhao, Zhihui Xu, Rongjuan Chen, Lanlan Si, Shanshan Lu, Xiaodong Li, Shuai Wang, Kai Zhang, Jin Li, Juqiang Han, Dongping Xu
OBJECTIVE: The impact of hepatitis B virus (HBV) preS/S-gene mutations on occult HBV infection (OBI) is not fully understood. This study characterized multiple novel HBV preS/S-gene mutants obtained from an OBI patient. METHODS: PreS/S-gene mutants were analyzed by clonal sequencing. Viral replication and expression were analyzed by transfecting HBV genomic recombinants into HepG2 cells. RESULTS: Twenty-one preS/S-gene mutants were cloned from four sequential serum samples, including 13 mutants that were not previously documented: (1) sI/T126V+sG145R; (2) preS1 nt 3014-3198 deletion; (3) preS1 nt 3046-3177 deletion; (4) preS1 nt 3046-3177 deletion+s115-116 "INGTST" insertion; (5) preS1 nt 3046-3177 deletion+s115-116 "INGTST" insertion+sG145R; (6) preS1 nt 3115-3123 deletion+sQ129N; (7) preS1 nt 3115-3123 deletion+s126-127 "RPCMNCTI" insertion; (8) s115-116 "INGTST" insertion; (9) s115-116 "INGTST" insertion+sG145R; (10) s126-127 "RPCMNCTI" insertion; (11) preS1 nt 2848-2862 deletion+preS2 initiation codon M→I; (12) s122-123 "KSTGLCK" insertion+sQ129N; and (13) preS2 initiation codon M→I+s131-133TSM→NST...
2016: PloS One
https://www.readbyqxmd.com/read/27084040/mechanisms-of-hbv-induced-hepatocellular-carcinoma
#17
REVIEW
Massimo Levrero, Jessica Zucman-Rossi
Hepatitis B virus (HBV) contributes to hepatocellular carcinoma (HCC) development through direct and indirect mechanisms. HBV DNA integration into the host genome occurs at early steps of clonal tumor expansion and induces both genomic instability and direct insertional mutagenesis of diverse cancer-related genes. Prolonged expression of the viral regulatory protein HBx and/or altered versions of the preS/S envelope proteins dysregulates cell transcription and proliferation control and sensitizes liver cells to carcinogenic factors...
April 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/27084035/overview-of-hepatitis-b-viral-replication-and-genetic-variability
#18
REVIEW
Shuping Tong, Peter Revill
Chronic infection with hepatitis B virus (HBV) greatly increases the risk for liver cirrhosis and hepatocellular carcinoma (HCC). HBV isolates worldwide can be divided into ten genotypes. Moreover, the immune clearance phase selects for mutations in different parts of the viral genome. The outcome of HBV infection is shaped by the complex interplay of the mode of transmission, host genetic factors, viral genotype and adaptive mutations, as well as environmental factors. Core promoter mutations and mutations abolishing hepatitis B e antigen (HBeAg) expression have been implicated in acute liver failure, while genotypes B, C, subgenotype A1, core promoter mutations, preS deletions, C-terminal truncation of envelope proteins, and spliced pregenomic RNA are associated with HCC development...
April 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/26905957/hepatitis-b-virus-full-length-genomic-mutations-and-quasispecies-in-hepatocellular-carcinoma
#19
An-Ye Zhang, Ching-Lung Lai, Ronnie Tung-Ping Poon, Fung-Yu Huang, Wai-Kay Seto, James Fung, Danny Ka-Ho Wong, Man-Fung Yuen
BACKGROUND AND AIM: Hepatitis B virus (HBV) full-length genomic mutations and quasispecies characteristics in hepatocellular carcinoma (HCC) were investigated. METHODS: Hepatitis B virus DNA was extracted from the tumor and non-tumor tissues of 16 HCC patients. Overlapping DNA fragments covering the entire HBV genome were amplified and sequenced. To study HBV sequence at the quasispecies level, the preS region was amplified and clonally sequenced. HBV mutation profiles, quasispecies complexity and diversity, and phylogenetic characteristics were assessed...
September 2016: Journal of Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/26877602/hepatocellular-carcinoma-and-hepatitis-b-surface-protein
#20
REVIEW
Yong-Wei Li, Feng-Cai Yang, Hui-Qiong Lu, Jiong-Shan Zhang
The tumorigenesis of hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) has been widely studied. HBV envelope proteins are important for the structure and life cycle of HBV, and these proteins are useful for judging the natural disease course and guiding treatment. Truncated and mutated preS/S are produced by integrated viral sequences that are defective for replication. The preS/S mutants are considered "precursor lesions" of HCC. Different preS/S mutants induce various mechanisms of tumorigenesis, such as transactivation of transcription factors and an immune inflammatory response, thereby contributing to HCC...
February 14, 2016: World Journal of Gastroenterology: WJG
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