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https://www.readbyqxmd.com/read/27913299/the-inhibitory-effect-of-beta-lapachone-on-rankl-induced-osteoclastogenesis
#1
Dong Ryun Gu, Joon No Lee, Gi-Su Oh, Hyung Jin Kim, Min Seuk Kim, Seoung Hoon Lee
β-lapachone (β-L) is a substrate of reduced nicotinamide adenine dinucleotide (NADH): quinone oxidoreductase 1 (NQO1). NQO1 reduces quinones to hydroquinones using NADH as an electron donor and consequently increases the intracellular NAD+/NADH ratio. The activation of NQO1 by β-L has beneficial effects on several metabolic syndromes, such as obesity, hypertension, and renal injury. However, the effect of β-L on bone metabolism remains unclear. Here, we show that β-L might be a potent inhibitor of receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis...
November 29, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27897222/nadph-oxidase-gp91-phox-contributes-to-rankl-induced-osteoclast-differentiation-by-upregulating-nfatc1
#2
In Soon Kang, Chaekyun Kim
Bone-marrow derived monocyte-macrophages (BMMs) differentiate into osteoclasts by M-CSF along subsequent RANKL stimulation possibly in collaboration with many other unknown cytokines released by pre- or mature osteoblasts. The differentiation process requires receptor activator of nuclear factor kappa-B ligand (RANKL)/RANK signaling and reactive oxygen species (ROS) such as superoxide anion (O2(•-)). Gp91(phox), a plasma membrane subunit of NADPH oxidase (Nox), is constitutively expressed in BMMs and plays a major role in superoxide anion production...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27896363/oxygen-ultra-fine-bubbles-water-administration-prevents-bone-loss-of-glucocorticoid-induced-osteoporosis-in-mice-by-suppressing-osteoclast-differentiation
#3
T Noguchi, K Ebina, M Hirao, T Morimoto, K Koizumi, K Kitaguchi, H Matsuoka, T Iwahashi, H Yoshikawa
: Oxygen ultra-fine bubbles (OUB) saline injection prevents bone loss of glucocorti\coid-induced osteoporosis in mice, and OUB inhibit osteoclastogenesis via RANK-TRAF6-c-Fos-NFATc1 signaling and RANK-p38 MAPK signaling in vitro. INTRODUCTION: Ultra-fine bubbles (<200 nm in diameter) have several unique properties, and they are tested in various medical fields. The purpose of this study was to investigate the effects of oxygen ultra-fine bubbles (OUB) on glucocorticoid-induced osteoporosis (GIO) model mice...
November 28, 2016: Osteoporosis International
https://www.readbyqxmd.com/read/27895178/conserved-region-c-functions-to-regulate-pd-1-expression-and-subsequent-cd8-t-cell-memory
#4
Alexander P R Bally, Yan Tang, Joshua T Lee, Benjamin G Barwick, Ryan Martinez, Brian D Evavold, Jeremy M Boss
Expression of programmed death 1 (PD-1) on CD8 T cells promotes T cell exhaustion during chronic Ag exposure. During acute infections, PD-1 is transiently expressed and has the potential to modulate CD8 T cell memory formation. Conserved region C (CR-C), a promoter proximal cis-regulatory element that is critical to PD-1 expression in vitro, responds to NFATc1, FoxO1, and/or NF-κB signaling pathways. Here, a CR-C knockout mouse was established to determine its role on PD-1 expression and the corresponding effects on T cell function in vivo...
November 28, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27879421/loss-of-notch3-signaling-enhances-osteogenesis-of-mesenchymal-stem-cells-from-mandibular-torus
#5
X W Dou, W Park, S Lee, Q Z Zhang, L R Carrasco, A D Le
Mandibular torus (MT) is a common intraoral osseous outgrowth located on the lingual surface of the mandible. Histologic features include hyperplastic bone consisting of mature cortical and trabecular bone. Some theories on the etiology of MT have been postulated, such as genetic factors, masticatory hyperfunction, trauma, and continued growth, but the underlying mechanism remains largely unknown. In this study, we investigated the potential role of mesenchymal stem cells (MSCs) derived from human MT in the pathogenesis of bone outgrowth...
November 22, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27871781/crocin-inhibits-rankl-induced-osteoclast-formation-and-bone-resorption-by-suppressing-nf-%C3%AE%C2%BAb-signaling-pathway-activation
#6
Lijia Fu, Fang Pan, Yong Jiao
Crocin is a dietary compound with antioxidant and anti-inflammatory properties, but its effects on bone resorption have not been well characterized. Here we address this issue by examining the direct effects of crocin on osteoclast cells in vitro. Osteoclastogenesis was induced by RANKL (receptor activator of NF-κB ligand) in mouse bone marrow-derived macrophages in the absence or presence of crocin at various concentrations. Further, the bone resorption activity of mature osteoclast treated with crocin was assessed by pit assay...
November 15, 2016: Immunobiology
https://www.readbyqxmd.com/read/27858552/fluvastatin-inhibits-osteoclast-differentiation-and-p-gingivalis-lipopolysaccharide-induced-alveolar-bone-erosion-in-mice
#7
Nitin Kumar Pokhrel, Yong-Gun Kim, Jae-Young Kim, Hong-Hee Kim, Youngkyun Lee
BACKGROUND: Statins have been widely used to treat hypercholesterolemia. In addition to the inhibition of cholesterol synthesis, recent reports suggest bone anabolic property of statins. However, little notice has been paid to the direct effect of statins on osteoclastogenesis and bone resorption. METHODS: The effect of fluvastatin on osteoclast differentiation was determined using in vitro culture of mouse bone marrow macrophages (BMMs) in the presence of macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappa B ligand (RANKL)...
November 18, 2016: Journal of Periodontology
https://www.readbyqxmd.com/read/27856256/pulsed-electromagnetic-field-inhibits-rankl-dependent-osteoclastic-differentiation-in-raw264-7-cells-through-the-ca-2-calcineurin-nfatc1-signaling-pathway
#8
Jie Zhang, Haixia Xu, Zhongyu Han, Ping Chen, Qiang Yu, Yutian Lei, Zongze Li, Ming Zhao, Jing Tian
Pulsed electromagnetic field (PEMF) has been reported to improve bone healing in osteoporosis patients. However, the precise mechanism has remained largely unknown. This study aimed to investigate the effects of PEMF on nuclear factor κB ligand (RANKL)-dependent osteoclastic differentiation and the Ca(2+)-calcineurin-NFATc1 signaling pathway in RAW264.7 cells in vitro. Treating RAW264.7 cells with RANKL for 4 days induced osteoclastic differentiation in vitro, and the formation of multinucleated osteoclasts, bone resorption-pit formation, tartrate-resistant acid phosphatase (TRAP) activity and the protein levels of cathepsin K, TRAP, Nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1) and matrix metalloproteinase 9 (MMP-9) were significantly decreased...
November 14, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27836739/docosahexaenoic-acid-signaling-attenuates-the-proliferation-and-differentiation-of-bone-marrow-derived-osteoclast-precursors-and-promotes-apoptosis-in-mature-osteoclasts
#9
Hyun-Ju Kim, Boram Ohk, Hye Jin Yoon, Woo Youl Kang, Sook Jin Seong, Shin-Yoon Kim, Young-Ran Yoon
Docosahexaenoic acid (DHA), a component of omega-3 fatty acids, has been reported to protect against inflammatory bone diseases such as osteoporosis and rheumatoid arthritis. However, its exact mechanism in bone resorbing cells has not been elucidated. In this study, we investigated the effects and the molecular mechanism of DHA on the proliferation, differentiation, and survival of osteoclast lineage cells using mouse bone marrow-derived macrophages (BMMs). DHA suppressed the macrophage colony-stimulating factor (M-CSF)-induced proliferation of osteoclast precursors, BMMs, in a dose-dependent manner...
November 9, 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27829167/atf3-modulates-calcium-signaling-in-osteoclast-differentiation-and-activity-by-associating-with-c-fos-and-nfatc1-proteins
#10
Byung-Chul Jeong, Jung Ha Kim, Kabsun Kim, Inyoung Kim, Semun Seong, Nacksung Kim
Activating transcription factor 3 (ATF3), a member of the ATF/cAMP response element-binding protein family of transcription factors, has been implicated in the regulation of cell proliferation and differentiation. However, whether ATF3 is involved in osteoclast differentiation and activity has not been well-studied. In the present study, we examined the role of ATF3 in osteoclast differentiation and function. ATF3 expression was down-regulated during RANKL-induced osteoclast differentiation. Overexpression of ATF3 in bone marrow-derived monocyte/macrophage lineage cells (BMMs) promoted osteoclast differentiation and activity and strongly induced the expression of osteoclast genes encoding nuclear factor of activated T-cells c1 (NFATc1) and tartrate-resistant acid phosphatase (TRAP) compared to that in the control group...
November 6, 2016: Bone
https://www.readbyqxmd.com/read/27821837/nitidine-chloride-prevents-ovx-induced-bone-loss-via-suppressing-nfatc1-mediated-osteoclast-differentiation
#11
Qian Liu, Tao Wang, Lin Zhou, Fangming Song, An Qin, Hao Tian Feng, Xi Xi Lin, Zhen Lin, Jin Bo Yuan, Jennifer Tickner, Hua Gang Liu, Ming Hao Zheng, Jiake Xu, Jin Min Zhao
Nitidine chloride (NC), a bioactive alkaloid isolated from Zanthoxylum nitidum, has been used as a herbal ingredient in toothpaste that prevents cavities for decades. It also displays potential antitumor and anti-inflammation properties. However, its anticatabolic effect on bone is not known. We investigated the effect of NC on osteoclastogenesis, bone resorption and RANKL-induced NF-κB and NFATc1 signalling. In mouse-derived bone marrow monocytes (BMMs), NC suppressed RANKL-induced multinucleated tartrate-resistant acid phosphatase (TRAP)-positive osteoclast formation and bone resorption in a dose dependent manner...
November 8, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27813153/traf6-mediates-suppression-of-osteoclastogenesis-and-prevention-of-ovariectomy-induced-bone-loss-by-a-novel-prenylflavonoid
#12
E M Tan, L Li, I R Indran, N Chew, E L Yong
Given the limitations of current therapeutic options for postmenopausal osteoporosis, there is a need for alternatives with minimal adverse effects. In this study, we evaluated the effects of icaritin (ICT), a natural prenylflavonoid, on osteoclastogenesis both in vitro and in an ovariectomized (OVX)-rat model, and investigated its underlying molecular mechanism(s) of action. ICT inhibited osteoclast formation in two osteoclast precursor models, RAW 264.7 mouse monocyte cell line and human PBMC. ICT also inhibited sealing zone and resorption pit formation in a dose-dependent manner...
November 4, 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/27807651/comparison-of-the-alendronate-and-irradiation-with-a-light-emitting-diode-led-on-murine-osteoclastogenesis
#13
Hong Moon Sohn, Youngjong Ko, Mineon Park, Bora Kim, Jung Eun Park, Donghwi Kim, Young Lae Moon, Wonbong Lim
Photomodulation therapy (PBMT) using light-emitting diode (LED) has been proposed as an alternative to conventional osteoporosis therapies. Our aim was to determine the effect of irradiation with a light-emitting diode on receptor activator of NF-κB ligand (RANKL)-mediated differentiation of mouse bone marrow macrophages into osteoclasts and compare it to alendronate treatment. The cells were irradiated with LED at 635±10 nm, 9-cm spot size, 5 mW/cm(2), and 18 J for 60 min/day in a CO2 incubator. The differentiation of irradiated and untreated RANKL-stimulated bone marrow macrophages into osteoclasts was evaluated by tartrate-resistant acid phosphatase (TRAP) staining and by molecular methods...
November 2, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27798154/icos-ligand-triggering-impairs-osteoclast-differentiation-and-function-in-vitro-and-in-vivo
#14
Casimiro L Gigliotti, Elena Boggio, Nausicaa Clemente, Yogesh Shivakumar, Erika Toth, Daniele Sblattero, Patrizia D'Amelio, Giovanni C Isaia, Chiara Dianzani, Junji Yagi, Josè M Rojo, Annalisa Chiocchetti, Renzo Boldorini, Michela Bosetti, Umberto Dianzani
Osteoblasts, osteocytes, and osteoclasts (OCs) are involved in the bone production and resorption, which are crucial in bone homeostasis. OC hyperactivation plays a role in the exaggerated bone resorption of diseases such as osteoporosis, rheumatoid arthritis, and osteolytic tumor metastases. This work stems from the finding that OCs can express B7h (ICOS-Ligand), which is the ligand of the ICOS T cell costimulatory molecule. Because recent reports have shown that, in endothelial, dendritic, and tumor cells, B7h triggering modulates several activities of these cells, we analyzed the effect of B7h triggering by recombinant ICOS-Fc on OC differentiation and function...
October 19, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27789216/artesunate-suppresses-rankl-induced-osteoclastogenesis-through-inhibition-of-plc%C3%AE-1-ca-2-nfatc1-signaling-pathway-and-prevents-ovariectomy-induced-bone-loss
#15
Xiangzhou Zeng, Yueyang Zhang, Song Wang, Keng Wang, Lei Tao, Min Zou, Nana Chen, Jiake Xu, Shuwen Liu, Xiaojuan Li
Bone lytic diseases including osteoporosis, rheumatoid arthritis, and bone metastatic tumors affect hundreds of millions people worldwide. Targeting over-activated osteoclasts as an anti-resorptive treatment becomes an important strategy to treat osteolytic diseases. Artesunate is a compound derived from artemisinin (qinghaosu) and has been used to treat malaria and rheumatoid arthritis clinically in China, but its role in osteolysis is unknown. Here, we found that artesunate could suppress RANKL-induced osteoclastogenesis and bone resorption from 1...
October 24, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27785371/vitamin-d-receptor-expression-in-human-bone-tissue-and-dose-dependent-activation-in-resorbing-osteoclasts
#16
Allahdad Zarei, Alireza Morovat, Kassim Javaid, Cameron P Brown
The effects of vitamin D on osteoblast mineralization are well documented. Reports of the effects of vitamin D on osteoclasts, however, are conflicting, showing both inhibition and stimulation. Finding that resorbing osteoclasts in human bone express vitamin D receptor (VDR), we examined their response to different concentrations of 25-hydroxy vitamin D3 [25(OH)D3] (100 or 500 nmol·L(-1)) and 1,25-dihydroxy vitamin D3 [1,25(OH)2D3] (0.1 or 0.5 nmol·L(-1)) metabolites in cell cultures. Specifically, CD14+ monocytes were cultured in charcoal-stripped serum in the presence of receptor activator of nuclear factor kappa-B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF)...
2016: Bone Research
https://www.readbyqxmd.com/read/27775174/clinically-relevant-concentrations-of-ketamine-inhibit-osteoclast-formation-in-vitro-in-mouse-bone-marrow-cultures
#17
Erxia Du, Patrick McAllister, Venugopal Reddy Venna, Liping Xiao
Ketamine has been used safely in clinics for decades for analgesia and anesthesia. It is increasingly popular in clinical practice due to its new uses and importance for emergency procedures. It is known that ketamine is sequestered in the bone marrow and the major receptors for ketamine, noncompetitive N-methyl-d-aspartate receptors (NMDARs), are expressed in osteoclasts (OCs) and osteoblasts. However, the impact of ketamine on OCs or osteoblasts is unknown. In this study, we investigated the effects of ketamine on osteoclastogenesis and regulation of NMDARs expression in vitro...
October 24, 2016: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/27765359/cardioprotective-actions-of-curcumin-on-the-pathogenic-nfat-cox-2-prostaglandin-e2-pathway-induced-during-trypanosoma-cruzi-infection
#18
Matías Hernández, Susana Wicz, Ricardo S Corral
BACKGROUND: Diverse cardiovascular signaling routes have been considered critical for Chagas cardiomyopathy caused by the protozoan parasite Trypanosoma cruzi. Along this line, T. cruzi infection and endothelin-1 (ET-1) have been shown to cooperatively activate the Ca(2+)/NFAT cascade in cardiomyocytes, leading to cyclooxygenase type 2 (COX-2) induction and increased release of prostanoids and prohypertrophic peptides. PURPOSE: To determine whether the well-known cardioprotective and anti-inflammatory effects of curcumin (Cur) could be helpful to interfere with this key machinery for pathogenesis of Chagas myocarditis...
November 15, 2016: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/27748817/release-from-optimal-compressive-force-suppresses-osteoclast-differentiation
#19
Masaaki Ikeda, Yoshitaka Yoshimura, Takashi Kikuiri, Mino Matsuno, Tomokazu Hasegawa, Kumu Fukushima, Takako Hayakawa, Hajime Minamikawa, Kuniaki Suzuki, Junichiro Iida
Bone remodeling is an important factor in orthodontic tooth movement. During orthodontic treatment, osteoclasts are subjected to various mechanical stimuli, and this promotes or inhibits osteoclast differentiation and fusion. It has been previously reported that the release from tensile force induces osteoclast differentiation. However, little is known about how release from compressive force affects osteoclasts. The present study investigated the effects of release from compressive force on osteoclasts. The number of tartrate‑resistant acid phosphatase (TRAP)‑positive multinucleated osteoclasts derived from RAW264...
October 5, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27746842/curcumin-reduces-the-noise-exposed-cochlear-fibroblasts-apoptosis
#20
Tengku Siti Hajar Haryuna, Wibi Riawan, Ardyansyah Nasution, Suprapto Ma'at, Juliandi Harahap, Indri Adriztina
Introduction The structural changes underlying permanent noise-induced hearing loss (NIHL) include loss of the sensory hair cells, damage to their stereocilia, and supporting tissues within the cochlear lateral wall. Objective The objective of this study is to demonstrate curcumin as a safe and effective therapeutic agent in the prevention and treatment for fibroblasts damage within the cochlear supporting tissues and lateral wall through cell death pathway. Methods We divided 24 Rattus norvegicus into 4 groups, Group 1: control; Group 2: noise (+); Group 3: noise (+), 50 mg/day curcumin (+); Group 4: noise (+), 100 mg/day curcumin (+)...
October 2016: International Archives of Otorhinolaryngology
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