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https://www.readbyqxmd.com/read/29781506/articular-cartilage-protection-in-ctsk-mice-is-associated-with-cellular-and-molecular-changes-in-subchondral-bone-and-cartilage-matrix
#1
Fabiana N Soki, Ryu Yoshida, David N Paglia, Le T Duong, Marc F Hansen, Hicham Drissi
Osteoarthritis (OA) is a degenerative disease and a major cause of chronic disability in aging individuals. Cathepsin K (CatK), encoded by the Ctsk gene, has been implicated in the pathogenesis of pycnodysostosis and osteoporosis. The use of a selective inhibitor of CatK was recently shown to delay OA progression in rabbits. However, the cellular mechanisms underlying these protective effects remain unexplored. We examined articular cartilage maintenance and joint bone remodeling using Ctsk null mice (Ctsk-/- ) which underwent destabilization of the medial meniscus (DMM)...
May 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29775898/particle-induced-sirt1-downregulation-promotes-osteoclastogenesis-and-osteolysis-through-er-stress-regulation
#2
Liang Zhang, Dongmei Bao, Peng Li, Zhidong Lu, Long Pang, Zhirong Chen, Haohui Guo, Zhihui Gao, Qunhua Jin
BACKGROUND: Sirtuin 1 (SIRT1) downregulation has been found to be induced by wear particles in aseptic prosthesis loosening (APL). Osteoclastogenesis and osteoclast activation are the main pathological factors associated with APL. However, whether SIRT1 downregulation contributes to the formation and activation of osteoclasts through the induction of endoplasmic reticulum (ER) stress is unclear. METHODS: To address this, an osteolysis mouse model was used in which animals were treated with the SIRT1 activator, resveratrol (RES), or an ER stress inhibitor, 4-PBA, for two weeks...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29775222/abca1-inhibits-pdgf-induced-proliferation-and-migration-of-rat-airway-smooth-muscle-cell-through-blocking-tlr2-nf-%C3%AE%C2%BAb-nfatc1-signaling
#3
Wei Cheng, Kun Yan, Yanni Chen, Wen Zhang, Zongzheng Ji, Chengxue Dang
Airway remodeling is a key feature of asthma, characterized by abnormal proliferation and migration of airway smooth muscle cells (ASMCs). ABCA1, a member of the ATP-binding cassette family of active transporters, plays an essential role in the progression of lung diseases. However, the contributions of ABCA1 in ASMCs remain to be explored. The purpose of the present study was to investigate the functional role and potential molecular mechanism of ABCA1 in platelet derived growth factor (PDGF)-induced primary rat ASMC proliferation and migration...
May 18, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29773595/therapeutic-effects-of-the-novel-subtype-selective-histone-deacetylase-hdac-inhibitor-chidamide-on-myeloma-associated-bone-disease
#4
Jingsong He, Qingxiao Chen, Huiyao Gu, Jing Chen, Enfan Zhang, Xing Guo, Xi Huang, Haimeng Yan, DongHua He, Yang Yang, Yi Zhao, Gang Wang, Huang He, Qing Yi, Zhen Cai
Histone deacetylases are promising therapeutic targets in hematological malignancies. In the present work, we investigated the effect of chidamide, a new subtype-selective histone deacetylase inhibitor that was independently produced in China, on multiple myeloma and its associated bone diseases using different models. The cytotoxicity of chidamide toward myeloma is due to its induction of cell apoptosis and cell cycle arrest by increasing the levels of caspase family proteins, including p21 and p27, among others...
May 17, 2018: Haematologica
https://www.readbyqxmd.com/read/29769195/osteocyte-driven-downregulation-of-snail-restrains-effects-of-drd2-inhibitors-on-mammary-tumor-cells
#5
Shengzhi Liu, Yao Fan, Andy Chen, Aydin Jalali, Kazumasa Minami, Kazuhiko Ogawa, Harikrishna Nakshatri, Bai-Yan Li, Hiroki Yokota
While bone is a frequent target of breast cancer-associated metastasis, little is known about the effects of tumor-bone interactions on the efficacy of tumor-suppressing agents. Here we examined the effect of two FDA-approved dopamine modulators, Fluphenazine (FP) and Trifluoperazine (TFP), on mammary tumor cells, osteoclasts, osteoblasts, and osteocytes. These agents suppressed proliferation and migration of mammary tumor cells chiefly by antagonizing dopamine receptor D2 and reduced bone resorption by downregulating nuclear factor of activated T-cells, cytoplasmic 1 (Nfatc1)...
May 16, 2018: Cancer Research
https://www.readbyqxmd.com/read/29763384/the-effects-of-dihydroartemisinin-on-inflammatory-bowel-disease-related-bone-loss-in-a-rat-model
#6
Xingtao Ge, Zhijian Chen, Zhenjie Xu, Fang Lv, Kewei Zhang, Yu Yang
Bone loss is one of the important extra-intestinal manifestations in patients with inflammatory bowel diseases (IBDs). Compounds derived from natural products have been used to treat IBDs. However, the role of natural products on IBD-induced bone loss is not completely clarified. In the present study, we observed the effects of dihydroartemisinin (DHA), an antimalaria drug, on IBD and IBD-induced bone loss in a rat model. Chronic IBD model was established in Sprague-Dawley rats by giving them 2.5% dextran sodium sulfate in drinking water...
May 2018: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29760402/roles-of-enhancer-rnas-in-rankl-induced-osteoclast-differentiation-identified-by-genome-wide-cap-analysis-of-gene-expression-using-crispr-cas9
#7
Yukako Sakaguchi, Keizo Nishikawa, Shigeto Seno, Hideo Matsuda, Hiroshi Takayanagi, Masaru Ishii
Bidirectional transcription has been proposed to play a role associated with enhancer activity. Transcripts called enhancer RNAs (eRNAs) play important roles in gene regulation; however, their roles in osteoclasts are unknown. To analyse eRNAs in osteoclasts comprehensively, we used cap-analysis of gene expression (CAGE) to detect adjacent transcription start sites (TSSs) that were distant from promoters for protein-coding gene expression. When comparing bidirectional TSSs between osteoclast precursors and osteoclasts, we found that bidirectional TSSs were located in the 5'-flanking regions of the Nrp2 and Dcstamp genes...
May 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29753717/trim38-regulates-nf-%C3%AE%C2%BAb-activation-through-tab2-degradation-in-osteoclast-and-osteoblast-differentiation
#8
Kabsun Kim, Jung Ha Kim, Inyoung Kim, Semun Seong, Nacksung Kim
The tripartite motif protein 38 (TRIM38), a member of the TRIM family, is involved in various cellular processes such as cell proliferation, differentiation, apoptosis, and antiviral defense. However, the role of TRIM38 in osteoclast and osteoblast differentiation is not yet known. In this study, we report the involvement of TRIM38 in osteoclast and osteoblast differentiation. Overexpression of TRIM38, in osteoclast precursor cells, attenuated receptor activator of nuclear factor kappa B ligand (RANKL)-induced osteoclast formation, RANKL-triggered NF-κB activation, and expression of osteoclast marker genes, such as NFATc1, osteoclast-associated receptor (OSCAR), and tartrate-resistant acid phosphatase (TRAP); and down-regulation of TRIM38 expression showed the opposite effects...
May 10, 2018: Bone
https://www.readbyqxmd.com/read/29752313/schistosomiasis-induces-persistent-dna-methylation-and-tuberculosis-specific-immune-changes
#9
Andrew R DiNardo, Tomoki Nishiguchi, Emily M Mace, Kimal Rajapakshe, Godwin Mtetwa, Alexander Kay, Gugu Maphalala, W Evan Secor, Rojelio Mejia, Jordan S Orange, Cristian Coarfa, Kapil N Bhalla, Edward A Graviss, Anna M Mandalakas, George Makedonas
Epigenetic mechanisms, such as DNA methylation, determine immune cell phenotype. To understand the epigenetic alterations induced by helminth coinfections, we evaluated the longitudinal effect of ascariasis and schistosomiasis infection on CD4+ T cell DNA methylation and the downstream tuberculosis (TB)-specific and bacillus Calmette-Guérin-induced immune phenotype. All experiments were performed on human primary immune cells from a longitudinal cohort of recently TB-exposed children. Compared with age-matched uninfected controls, children with active Schistosoma haematobium and Ascaris lumbricoides infection had 751 differentially DNA-methylated genes, with 72% hypermethylated...
May 11, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29749669/in-silico-analysis-of-single-cell-rna-sequencing-data-from-3-and-7-days-old-mouse-spermatogonial-stem-cells-to-identify-their-differentially-expressed-genes-and-transcriptional-regulators
#10
Sajjad Sisakhtnezhad
Spermatogonial stem cells (SSCs), which are at the basis of spermatogenesis process, are valuable cells with different applications in biotechnology and regenerative medicine. Understanding the molecular basis of SSC self-renewal and differentiation at various developmental stages of the male organism is crucial to find key factors in the SSCs fate and function. Therefore, this study was aimed to use single-cell RNA-sequencing dataset analysis for identification of differentially expressed genes (DEGs) and their regulators in 3 and 7 days old mouse-derived single SSCs (mSSCs)...
May 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29747758/inhibitory-effect-of-cudratricusxanthone-a-on-osteoclast-differentiation-and-function
#11
Eun-Hwa Choi, Eun-Nam Kim, Gil-Saeong Jeong
BACKGROUND: Cudratricusxanthone A (CTXA) was isolated from Cudrania tricuspidata and its anti-inflammatory, hepatoprotective, and anti-proliferative activities have previously been studied in vitro. However, effects of CTXA on osteoclast differentiation have not been investigated. PURPOSE: In this study, the effect of CTXA from C. tricuspidata on in vitro osteoclastogenesis was studied. DESIGN/METHODS: CTXA was isolated from the roots of C...
April 1, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/29737562/mechanisms-involved-in-enhancement-of-osteoclast-formation-by-activin-a
#12
Tomonari Kajita, Wataru Ariyoshi, Toshinori Okinaga, Sho Mitsugi, Kazuhiro Tominaga, Tatsuji Nishihara
Several growth factors in bone tissues are reported to be associated with osteoclastogenesis. Activin-A, a member of the transforming growth factor-β (TGF-β) family is known to be present in bone tissues and an important regulator in osteoclastogenesis with SMAD-mediated signaling being crucial for inducing osteoclast differentiation. In the present study, we examined the effect and underlying mechanisms of activin-A on osteoclast formation in vitro culture systems. Activin-A enhanced osteoclast formation in both mouse bone marrow cells and monocyte/macrophage cell line RAW 264...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29725523/suppression-of-osteoclastogenesis-via-%C3%AE-2-adrenergic-receptors
#13
Kosuke Hamajima, Kazunori Hamamura, Andy Chen, Hiroki Yokota, Hironori Mori, Shoyoku Yo, Hisataka Kondo, Kenjiro Tanaka, Kyoko Ishizuka, Daisuke Kodama, Takao Hirai, Ken Miyazawa, Shigemi Goto, Akifumi Togari
The sympathetic nervous system is known to regulate osteoclast development. However, the involvement of α2-adrenergic receptors (α2-ARs) in osteoclastogenesis is not well understood. In the present study, their potential role in osteoclastogenesis was investigated. Guanabenz, clonidine and xylazine were used as agonists of α2-ARs, while yohimbine and idazoxan were employed as antagonists. Using RAW264.7 pre-osteoclast and primary bone marrow cells, the mRNA expression of the osteoclast-related genes nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), tartrate-resistant acid phosphatase (TRAP) and cathepsin K was evaluated following induction with receptor activator of nuclear factor κB ligand (RANKL)...
May 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29724825/deletion-of-ferroportin-in-murine-myeloid-cells-increases-iron-accumulation-and-stimulates-osteoclastogenesis-in-vitro-and-in-vivo
#14
Lei Wang, Bin Fang, Toshifumi Fujiwara, Kimberly Krager, Akshita Gorantla, Chaoyuan Li, Jian Q Feng, Michael L Jennings, Jian Zhou, Nukhet Aykin-Burns, Haibo Zhao
Osteoporosis, osteopenia, and pathological bone fractures are frequent complications of iron-overload conditions such as hereditary hemochromatosis, thalassemia, and sickle cell disease. Moreover, animal models of iron overload have revealed increased bone resorption and decreased bone formation. Although systemic iron overload affects multiple organs and tissues, leading to significant changes on bone modeling and remodeling, the cell autonomous effects of excessive iron on bone cells remain unknown. Here, to elucidate the role of cellular iron homeostasis in osteoclasts, we generated two mouse strains in which solute carrier family 40 member 1 (Slc40a1), a gene encoding ferroportin (FPN), the sole iron exporter in mammalian cells, were specifically deleted in myeloid osteoclast precursors or mature cells...
May 3, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29721581/glycyrrhizin-suppresses-rankl-induced-osteoclastogenesis-and-oxidative-stress-through-inhibiting-nf-%C3%AE%C2%BAb-and-mapk-and-activating-ampk-nrf2
#15
Zhikun Li, Chao Chen, Xiaodong Zhu, Yifan Li, Ronghua Yu, Wei Xu
The treatment for osteoporosis involves inhibiting bone resorption and osteoclastogenesis. Glycyrrhizin (GLY) is a triterpenoid saponin glycoside known to be as the most medically efficacious component of the licorice plant. It has strong anti-inflammatory, antioxidant, and antitumor properties. We investigated the effect of GLY on osteoclastogenesis, bone resorption, and intracellular oxidative stress and its molecular mechanisms. In vitro osteoclastogenesis assays were performed using bone marrow monocytes with and without glycyrrhizin...
May 2, 2018: Calcified Tissue International
https://www.readbyqxmd.com/read/29720166/inhibitory-effects-of-sodium-pentosan-polysulfate-on-formation-and-function-of-osteoclasts-derived-from-canine-bone-marrow
#16
H M Suranji Wijekoon, Eugene C Bwalya, Jing Fang, Sangho Kim, Kenji Hosoya, Masahiro Okumura
BACKGROUND: Sodium pentosan polysulfate (NaPPS) was testified as a chondroprotective drug in with a detailed rationale of the disease-modifying activity. This study was undertaken to determine whether anti-osteoarthritis drug, NaPPS inhibited osteoclasts (OC) differentiation and function. Canine bone marrow mononuclear cells (n = 6) were differentiated to OC by maintaining with receptor activator of nuclear factor kappa B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) for up to 7 days with the treatment of NaPPS at concentration of 0, 0...
May 2, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29709064/effects-of-moderate-intensity-static-magnetic-fields-on-osteoclastic-differentiation-in-mouse-bone-marrow-cells
#17
Eun-Cheol Kim, Jaesuh Park, Gunwoo Noh, Su-Jung Park, Kwantae Noh, Il-Keun Kwon, Su-Jin Ahn
Although we recently demonstrated that static magnetic fields (SMFs) of 3, 15, and 50 mT stimulate osteoblastic differentiation, the effects of SMFs on osteoclastogenesis are still poorly understood. This study focused on the suppressive effects of SMFs on receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis and bone resorption. Direct SMFs inhibit RANKL-induced multinucleated osteoclast formation, tartrate-resistant acid phosphatase activity, and bone resorption in mouse bone marrow-derived macrophage cells...
April 30, 2018: Bioelectromagnetics
https://www.readbyqxmd.com/read/29705696/microrna-139-5p-suppresses-myosin-heavy-chain-i-and-iia-expression-via-inhibition-of-the-calcineurin-nfat-signaling-pathway
#18
Meng Xu, Xiaoling Chen, Zhiqing Huang, Daiwen Chen, Bing Yu, Hong Chen, Jun He, Ping Zheng, Junqiu Luo, Jie Yu, Yuheng Luo
MicroRNAs (miRNAs) are a class of small non-coding RNAs that are widely involved in a variety of biological processes. Different skeletal muscle fiber type composition exhibits characteristic differences in functional properties and energy metabolism of skeletal muscle. However, the molecular mechanism by which miRNAs control the different type of muscle fiber formation is still not fully understood. In the present study, we characterized the role of microRNA-139-5p (miR-139-5p) in the regulation of myosin heavy chain (MyHC) isoform expression and its underlying mechanisms...
April 26, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29703642/oleanolic-acid-exerts-bone-protective-effects-in-ovariectomized-mice-by-inhibiting-osteoclastogenesis
#19
Dongfeng Zhao, Xiaofeng Li, Yongjian Zhao, Ping Qiao, Dezhi Tang, Yan Chen, Chunchun Xue, Chenguang Li, Shufen Liu, Jing Wang, Sheng Lu, Qi Shi, Yan Zhang, Yufeng Dong, Yongjun Wang, Bing Shu, Xu Feng
Postmenopausal osteoporosis (POP) is quite prevalent and many new drugs are under development to obtain better therapeutic outcomes. Oleanolic acid (OA) has been reported to prevent bone loss in ovariectomized (OVX) rats by stimulating osteoblastogenesis. One previous study has demonstrated that acetate of OA suppressed lipopolysaccharides (LPS)-induced bone loss in mice. However, the role of OA in the receptor activator of nuclear factor kappa-B ligand (RANKL)-mediated osteoclastogenesis is still not elucidated...
April 6, 2018: Journal of Pharmacological Sciences
https://www.readbyqxmd.com/read/29698379/neogambogic-acid-suppresses-receptor-activator-of-nuclear-factor-%C3%AE%C2%BAb-ligand-rankl-induced-osteoclastogenesis-by-inhibiting-the-jnk-and-nf-%C3%AE%C2%BAb-pathways-in-mouse-bone-marrow-derived-monocyte-macrophages
#20
Gu Jin, Fang-Fang Wang, Tao Li, Dong-Dong Jia, Yong Shen, Hai-Chao Xu
BACKGROUND Neogambogic acid (NGA) is used in traditional Chinese medicine. The aim of this study was to investigate the effects of NGA on gene signaling pathways involved in osteoclastogenesis in mouse bone marrow-derived monocyte/macrophages (BMMs) and on bone resorption in vitro. MATERIAL AND METHODS Primary mouse BMMs were cultured with increasing concentrations of NGA. Real-time polymerase chain reaction was used to study the expression of mRNAs corresponding to gene products specific to receptor activator of NF-κB ligand (RANKL)-induced osteoclast differentiation, including tartrate-resistant acid phosphatase (TRAP), calcitonin receptor (CTR), cathepsin K (CTSK), and nuclear factor of activated T cells c1 (NFATc1)...
April 26, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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