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Adipose derived regenerative cells

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https://www.readbyqxmd.com/read/28718680/development-of-emu-oil-loaded-pcl-collagen-bioactive-nanofibers-for-proliferation-and-stemness-preservation-of-human-adipose-derived-stem-cell-possible-application-in-regenerative-medicine
#1
Kazem Nejati-Koshki, Younes Pilehvar-Soltanahmadi, Effat Alizadeh, Abbas Ebrahimi-Kalan, Yousef Mortazavi, Nosratollah Zarghami
Adipose tissue-derived stem cells (ASCs) are promising candidate in stem cell therapies, and maintaining their stemness potential is vital to achieve effective treatment. Natural based scaffolds have been recently attracted increasing attention in nanomedicine and drug delivery. In the present study, a polymeric nanofibrous scaffold was developed based on the polycaprolactone/Collagen (PCL/Coll) containing Emu oil as a bioactive material to induce the proliferation of ASCs, while simultaneously preserve the stemness property of those cells...
July 18, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28707770/comparison-of-the-paracrine-activity-of-mesenchymal-stem-cells-derived-from-human-umbilical-cord-amniotic-membrane-and-adipose-tissue
#2
Filip A Dabrowski, Anna Burdzinska, Agnieszka Kulesza, Anna Sladowska, Aleksandra Zolocinska, Kamila Gala, Leszek Paczek, Miroslaw Wielgos
AIM: The study was conducted to investigate secretory activity and define the paracrine potential of mesenchymal stem cells from human umbilical cord and amniotic membrane (UC-MSCs and AM-MSCs, respectively). METHODS: UC-MSCs (n = 6) were obtained from tissue explants using an adherent method after two weeks of incubation. AM-MSCs (n = 6) were obtained by digestion with tripsin and collagenase. MSC phenotype was confirmed in vitro by performing flow cytometry, differentiation assays and vimentin staining...
July 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/28705238/interleukin-3-enhances-the-migration-of-human-mesenchymal-stem-cells-by-regulating-expression-of-cxcr4
#3
Amruta Barhanpurkar-Naik, Suhas T Mhaske, Satish T Pote, Kanupriya Singh, Mohan R Wani
BACKGROUND: Mesenchymal stem cells (MSCs) represent an important source for cell therapy in regenerative medicine. MSCs have shown promising results for repair of damaged tissues in various degenerative diseases in animal models and also in human clinical trials. However, little is known about the factors that could enhance the migration and tissue-specific engraftment of exogenously infused MSCs for successful regenerative cell therapy. Previously, we have reported that interleukin-3 (IL-3) prevents bone and cartilage damage in animal models of rheumatoid arthritis and osteoarthritis...
July 14, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28705162/the-effect-of-autologous-adipose-derived-mesenchymal-stem-cell-therapy-in-the-treatment-of-a-large-osteochondral-defect-of-the-knee-following-unsuccessful-surgical-intervention-of-osteochondritis-dissecans-a-case-study
#4
Julien Freitag, Kiran Shah, James Wickham, Richard Boyd, Abi Tenen
BACKGROUND: A prospective analysis of the effect of autologous adipose derived mesenchymal stem cell (MSC) therapy in the treatment of an osteochondral defect of the knee with early progressive osteoarthritis following unsuccessful surgical intervention of osteochondritis dissecans (OCD). CASE PRESENTATION: After failed conventional management of OCD a patient undergoes intra-articular MSC therapy. Patient outcome measures included the Numeric Pain Rating Scale (NPRS), the Western Ontario and McMaster Universities Arthritis Index (WOMAC) and the Knee Injury and Osteoarthritis Outcome Score (KOOS)...
July 14, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/28702186/the-biological-and-clinical-basis-for-the-use-of-adipose-derived-stem-cells-in-the-field-of-wound-healing
#5
REVIEW
Nicolò Bertozzi, Francesco Simonacci, Michele Pio Grieco, Eugenio Grignaffini, Edoardo Raposio
Worldwide, hard-to-heal lower limb wounds are estimated to affect 1.5-3% of the adult population with a treatment-related annual cost of $10 billion. Thus, chronic skin ulcers of the lower limb are a matter of economic and public concern. Over the years, multiple medical and surgical approaches have been proposed but they are still inadequate, and no effective therapy yet exists. Regenerative medicine and stem cell-based therapies hold great promise for wound healing. Recently, many plastic surgeons have studied the potential clinical application of adipose-derived stem cells (ASCs), which are a readily available adult stem cell population that can undergo multilineage differentiation and secrete growth factors that can enhance wound-healing processes by promoting angiogenesis, and hence increase local blood supply...
August 2017: Annals of Medicine and Surgery
https://www.readbyqxmd.com/read/28697880/comparison-of-autologous-bone-marrow-and-adipose-tissue-derived-mesenchymal-stem-cells-and-platelet-rich-plasma-for-treating-surgically-induced-lesions-of-the-equine-superficial-digital-flexor-tendon
#6
A Romero, L Barrachina, B Ranera, A R Remacha, B Moreno, I de Blas, A Sanz, F J Vázquez, A Vitoria, C Junquera, P Zaragoza, C Rodellar
Several therapies have been investigated for equine tendinopathies, but satisfactory long term results have not been achieved consistently and a better understanding of the healing mechanism elicited by regenerative therapies is needed. The aim of this study was to assess the separate effects of autologous bone marrow (BM) and adipose tissue (AT) derived mesenchymal stem cells (MSCs), and platelet rich plasma (PRP), for treating lesions induced in the superficial digital flexor tendon (SDFT) of horses. Lesions were created surgically in both SDFTs of the forelimbs of 12 horses and were treated with BM-MSCs (six tendons), AT-MSCs (six tendons) or PRP (six tendons)...
June 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28695751/watercress-based-electrospun-nanofibrous-scaffolds-enhance-proliferation-and-stemness-preservation-of-human-adipose-derived-stem-cells
#7
Mehdi Dadashpour, Younes Pilehvar-Soltanahmadi, Seyed Abolghasem Mohammadi, Nosratollah Zarghami, Mohammad Pourhassan-Moghaddam, Effat Alizadeh, Mohammad Jafar Maleki, Akram Firouzi-Amandi, Mohammad Nouri
The present study describes the effects of Watercress extract (WE) based electrospun nanofibrous mats on the regulation of adhesion, proliferation, cytoprotection and stemness preservation of adipose-derived stem cells (ADSCs). Watercress (Nasturtium officinale) is one of the most important medicinal plant with a board spectrum of biological functions. For this purpose, WE-loaded PCL-PEG nanofibers were fabricated by electrospinning and characterized using FE-SEM and FTIR. Adhesion, proliferation and cytoprotection of ADSCs on the nanofibers was investigated using FE-SEM and MTT assays...
July 11, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28686790/adipose-stem-cells-enhance-myoblast-proliferation-via-acetylcholine-and-erk1-2-signaling
#8
Roine El-Habta, Paul J Kingham, Ludvig J Backman
Introduction We investigated the interaction between adipose tissue derived stem cells (ASCs) and myoblasts in co-culture experiments. Methods Specific inductive media were used to differentiate ASCs in vitro into a Schwann cell-like phenotype (dASCs), and subsequently the expression of acetylcholine (ACh) related machinery was determined. Furthermore, the expression of muscarinic ACh receptors (mAChRs) was examined in denervated rat gastrocnemius muscles. Results In contrast to undifferentiated ASCs, dASCs expressed more choline acetyltransferase (ChAT) and vesicular acetylcholine transporter (VAChT)...
July 7, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/28683107/human-bone-marrow-harbors-cells-with-neural-crest-associated-characteristics-like-human-adipose-and-dermis-tissues
#9
Cécile Coste, Virginie Neirinckx, Anil Sharma, Gulistan Agirman, Bernard Rogister, Jacques Foguenne, François Lallemend, André Gothot, Sabine Wislet
Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC...
2017: PloS One
https://www.readbyqxmd.com/read/28678424/the-metabolic-syndrome-alters-the-mirna-signature-of-porcine-adipose-tissue-derived-mesenchymal-stem-cells
#10
Yu Meng, Alfonso Eirin, Xiang-Yang Zhu, Hui Tang, Pritha Chanana, Amir Lerman, Andre J Van Wijnen, Lilach O Lerman
Autologous transplantation of mesenchymal stem cells (MSCs) is a viable option for the treatment of several diseases. Evidence indicates that MSCs release extracellular vesicles (EVs) and that EVs shuttle miRNAs to damaged parenchymal cells to activate an endogenous repair program. We hypothesize that comorbidities may interfere with the packaging of cargo in MSC-derived EVs. Therefore, we examined whether metabolic syndrome (MetS) modulates the miRNA content packed within MSC-derived EVs. MSCs were collected from swine abdominal adipose tissue after 16 weeks of lean or obese diet (n = 7 each)...
July 5, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28653440/treatment-of-breast-cancer-related-lymphedema-with-adipose-derived-regenerative-cells-and-fat-grafts-a-feasibility-and-safety-study
#11
Navid Mohamadpour Toyserkani, Charlotte Harken Jensen, Ditte Caroline Andersen, Søren Paludan Sheikh, Jens Ahm Sørensen
Breast cancer-related lymphedema (BCRL) is a debilitating late complication with a lack of treatment opportunities. Recent studies have suggested that mesenchymal stromal cells can alleviate lymphedema. Herein, we report the results from the first human pilot study with freshly isolated adipose-derived regenerative cells (ADRC) for treating lymphedema with 6 months follow-up. Ten BCRL patients were included. ADRC was injected directly into the axillary region, which was combined with a scar-releasing fat graft procedure...
June 27, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28622604/tissue-engineered-hypertrophic-chondrocyte-grafts-enhanced-long-bone-repair
#12
Jonathan Bernhard, James Ferguson, Bernhard Rieder, Patrick Heimel, Thomas Nau, Stefan Tangl, Heinz Redl, Gordana Vunjak-Novakovic
Bone has innate ability to regenerate following injury. However, large and complex fractures exceed bone's natural repair capacity and result in non-unions, requiring external intervention to facilitate regeneration. One potential treatment solution, tissue-engineered bone grafts, has been dominated by recapitulating intramembranous ossification (bone formation by osteoblasts), although most serious bone injuries heal by endochondral ossification (bone formation by remodeling of hypertrophic cartilaginous anlage)...
September 2017: Biomaterials
https://www.readbyqxmd.com/read/28619097/adipose-tissue-derived-stromal-vascular-fraction-in-regenerative-medicine-a-brief-review-on-biology-and-translation
#13
REVIEW
Pablo Bora, Anish S Majumdar
Adipose/fat tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration and can also give rise to a substantial number of cultured, multipotent adipose-derived stromal cells (ADSCs). Recently, both SVF and ADSCs have gained wide-ranging translational significance in regenerative medicine. Initially used for cosmetic breast enhancement, this mode of treatment has found use in many diseases involving immune disorders, tissue degeneration, and ischaemic conditions. In this review, we try to address several important aspects of this field, outlining the biology, technology, translation, and challenges related to SVF- and ADSC-based therapies...
June 15, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28618254/platelet-derived-growth-factor-bb-influences-muscle-regeneration-in-duchenne-muscle-dystrophy
#14
Patricia Piñol-Jurado, Eduard Gallardo, Noemi de Luna, Xavier Suárez-Calvet, Carles Sánchez-Riera, Esther Fernández-Simón, Clara Gomis, Isabel Illa, Jordi Díaz-Manera
Duchenne muscular dystrophy (DMD) is characterized by a progressive loss of muscle fibers, and their substitution by fibrotic and adipose tissue. Many factors contribute to this process, but the molecular pathways related to regeneration and degeneration of muscle are not completely known. Platelet-derived growth factor (PDGF)-BB belongs to a family of growth factors that regulate proliferation, migration, and differentiation of mesenchymal cells. The role of PDGF-BB in muscle regeneration in humans has not been studied...
June 12, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28610961/histone-acetylation-favours-the-cardiovascular-commitment-of-adipose-tissue-derived-stromal-cells
#15
Elena De Falco, Antonella Bordin, Eleonora Scaccia, Francesca Pagano, Mohsen Ibrahim, Leonardo Schirone, Francesco Angelini, Silvia Palmerio, Michele Madonna, Luca Fianchini, Isotta Chimenti, Sebastiano Sciarretta, Giacomo Frati
BACKGROUND: Although adipose stromal cells (ASCs) retain the ability to transdifferentiate at low rate towards the cardiac lineage, the potential mechanisms underlying such process have still to be elucidated. METHODS: Since chromatin state modifications are involved in several processes regulating the cellular cell fate commitment, we aimed at evaluating the role of histone protein acetylation in the cardiovascular-like transdifferentiation of ASCs. RESULTS: We found a clear increase of histone 3 acetylation status paralleled by a significant upregulation of cardiac TnI gene expression, in ASCs treated with the conditioned medium of primary cardiomyocyte cell cultures for 72h...
June 2, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28595566/a-systemic-review-of-the-sources-of-adult-mesenchymal-stem-cells-and-their-suitability-in-musculoskeletal-applications
#16
Rhiannon Nancarrow-Lei, Pouya Mafi, Reza Mafi, Wasim Khan
Background: Adult mesenchymal stem cells (MSCs) were first isolated from bone marrow by Friedenstein in 1976. These cells were clonogenic, non-haematopoietic, and able to replicate extensively in vitro. The fields of regenerative medicine and tissue engineering have grown dramatically since their inception. In the decades since, MSCs have been identified from mesoderm-, endoderm- and ectoderm-derived tissues. In light of our ageing population, the need for effective cell-based therapies for tissue repair and regeneration is ever-expanding...
June 8, 2017: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28593712/human-adipose-tissue-derived-tenomodulin-positive-subpopulation-of-stem-cells-a-promising-source-of-tendon-progenitor-cells
#17
A I Gonçalves, P M Gershovich, M T Rodrigues, R L Reis, M E Gomes
Cell-based therapies are of particular interest for tendon and ligament regeneration given the low regenerative potential of these tissues. Adipose tissue is an abundant source of stem cells, which may be employed for the healing of tendon lesions. However, human adult multipotent adipose-derived stem cells (hASCs) isolated from the stromal vascular fraction (SVF) of adipose tissue originate highly heterogeneous cell populations that hinder their use in specific tissue-oriented applications. In this study, distinct subpopulations of hASCs were immunomagnetic separated and their tenogenic differentiation capacity evaluated in the presence of several growth factors (GFs), namely EGF, bFGF, TGF-β1 and PDGF-BB, that are well-known regulators of tendon development, growth and healing...
June 7, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28592806/platelet-rich-plasma-respectively-reduces-and-promotes-adipogenic-and-myofibroblastic-differentiation-of-human-adipose-derived-stromal-cells-via-the-tgf%C3%AE-signalling-pathway
#18
Bérengère Chignon-Sicard, Magali Kouidhi, Xi Yao, Audrey Delerue-Audegond, Phi Villageois, Pascal Peraldi, Patricia Ferrari, Yves Rival, David Piwnica, Jérôme Aubert, Christian Dani
Autologous fat grafting is a gold standard therapy for soft tissue defects, but is hampered by unpredictable postoperative outcomes. Fat graft enrichment with adipose-derived stromal cell (ASCs) was recently reported to enhance graft survival. Platelet-rich plasma (PRP) has also emerged as a biologic scaffold that promotes fat graft viability. Combined ASC/PRP fat grafting enrichment is thus a promising new regenerative medicine approach. The effects of PRP on ASC proliferation are well documented, but the impact of PRP on ASC differentiation has yet to be investigated in depth to further elucidate the PRP clinical effects...
June 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28592171/next-generation-human-skin-constructs-as-advanced-tools-for-drug-development
#19
H E Abaci, Zongyou Guo, Yanne Doucet, Joanna Jacków, Angela Christiano
Many diseases, as well as side effects of drugs, manifest themselves through skin symptoms. Skin is a complex tissue that hosts various specialized cell types and performs many roles including physical barrier, immune and sensory functions. Therefore, modeling skin in vitro presents technical challenges for tissue engineering. Since the first attempts at engineering human epidermis in 1970s, there has been a growing interest in generating full-thickness skin constructs mimicking physiological functions by incorporating various skin components, such as vasculature and melanocytes for pigmentation...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28589682/expansion-of-adipose-tissue-derived-stromal-cells-at-physiologic-hypoxia-attenuates-replicative-senescence
#20
Andrey Ratushnyy, Margarita Lobanova, Ludmila B Buravkova
Multipotent mesenchymal stromal cells are considered as a perspective tool in cell therapy and regenerative medicine. Unfortunately, autologous cell therapy does not always provide positive outcomes in elder donors, perhaps as a result of the alterations of stem cell compartments. The mechanisms of stem and progenitor cell senescence and the factors engaged are investigated intensively. In present paper, we elucidated the effects of tissue-related O2 on morphology, functions, and transcriptomic profile of adipose tissue-derived stromal cells (ASCs) in replicative senescence in vitro model...
June 2017: Cell Biochemistry and Function
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