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Adipose derived regenerative cells

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https://www.readbyqxmd.com/read/28932116/silk-fibroin-cartilage-extracellular-matrix-scaffolds-with-sequential-delivery-of-tgf-%C3%AE-3-for-chondrogenic-differentiation-of-adipose-derived-stem-cells
#1
Qiang Yang, Bin-Hong Teng, Li-Na Wang, Kun Li, Chen Xu, Xin-Long Ma, Yang Zhang, De-Ling Kong, Lian-Yong Wang, Yan-Hong Zhao
A 3-D scaffold that simulates the microenvironment in vivo for regenerating cartilage is ideal. In this study, we combined silk fibroin and decellularized cartilage extracellular matrix by temperature gradient-guided thermal-induced phase separation to produce composite scaffolds (S/D). Resulting scaffolds had remarkable mechanical properties and biomimeticstructure, for a suitable substrate for attachment and proliferation of adipose-derived stem cells (ADSCs). Moreover, transforming growth factor β3 (TGF-β3) loaded on scaffolds showed a controlled release profile and enhanced the chondrogenic differentiation of ADSCs during the 28-day culture...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28928907/adipose-derived-stromal-cell-in-regenerative-medicine-a-review
#2
REVIEW
Reza Tabatabaei Qomi, Mohsen Sheykhhasan
The application of appropriate cell origin for utilizing in regenerative medicine is the major issue. Various kinds of stem cells have been used for the tissue engineering and regenerative medicine. Such as, several stromal cells have been employed as treat option for regenerative medicine. For example, human bone marrow-derived stromal cells and adipose-derived stromal cells (ADSCs) are used in cell-based therapy. Data relating to the stem cell therapy and processes associated with ADSC has developed remarkably in the past 10 years...
August 26, 2017: World Journal of Stem Cells
https://www.readbyqxmd.com/read/28927930/biodegradable-and-adjustable-sol-gel-glass-based-hybrid-scaffolds-from-multi-armed-oligomeric-building-blocks
#3
Christian Kascholke, Stephan Hendrikx, Tobias Flath, Dzmitry Kuzmenka, Hans-Martin Dörfler, Dirk Schumann, Mathias Gressenbuch, F Peter Schulze, Michaela Schulz-Siegmund, Michael C Hacker
Biodegradability is a crucial characteristic to improve the clinical potential of sol-gel-derived glass materials. To this end, a set of degradable organic/inorganic class II hybrids from a tetraethoxysilane(TEOS)-derived silica sol and oligovalent cross-linker oligomers containing oligo(D,L-lactide) domains was developed and characterized. A series of 18 oligomers (Mn: 1100-3200 Da) with different degrees of ethoxylation and varying length of oligoester units was established and chemical composition was determined...
September 16, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28919507/tissue-engineered-magnetic-cell-sheet-patches-for-advanced-strategies-in-tendon-regeneration
#4
Ana I Gonçalves, Márcia T Rodrigues, Manuela E Gomes
Tendons are powerful 3D biomechanically structures combining a few cells in an intrincated and highly hierarchical niche environment. When tendon homeostasis is compromised, restoration of functionality upon injury is limited and requires alternatives to current augmentation or replacement strategies. Cell sheet technologies are a powerful tool for the fabrication of living extracellular-rich patches towards regeneration of tenotopic defects. Thus, we originally propose the development of magnetically responsive tenogenic patches through magnetic cell sheet (magCSs) technology that enable the remote control upon implantation of the tendon-mimicking constructs...
September 14, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28917626/ultrasound-assisted-liposuction-provides-a-source-for-functional-adipose-derived-stromal-cells
#5
Dominik Duscher, Zeshaan N Maan, Anna Luan, Matthias M Aitzetmüller, Elizabeth A Brett, David Atashroo, Alexander J Whittam, Michael S Hu, Graham G Walmsley, Khosrow S Houschyar, Arndt F Schilling, Hans-Guenther Machens, Geoffrey C Gurtner, Michael T Longaker, Derrick C Wan
BACKGROUND AIMS: Regenerative medicine employs human mesenchymal stromal cells (MSCs) for their multi-lineage plasticity and their pro-regenerative cytokine secretome. Adipose-derived mesenchymal stromal cells (ASCs) are concentrated in fat tissue, and the ease of harvest via liposuction makes them a particularly interesting cell source. However, there are various liposuction methods, and few have been assessed regarding their impact on ASC functionality. Here we study the impact of the two most popular ultrasound-assisted liposuction (UAL) devices currently in clinical use, VASER (Solta Medical) and Lysonix 3000 (Mentor) on ASCs...
September 13, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28905449/lull-pgm-system-a-suitable-technique-to-improve-the-regenerative-potential-of-autologous-fat-grafting
#6
Paolo G Morselli, Federico A Giorgini, Claudia Pazzini, Claudio Muscari
Autologous fat grafting and methods of purification of harvested tissue have become one of the most current themes in regenerative medicine. The aim of this study was to evaluate the in vitro regenerative potential of abdomen lipoaspirates subjected to a combined washing-decantation purifying procedure, the Lull pgm System (Lull). Blood cells and stromal-vascular fraction (SVF) cells contained in the aspirates were investigated and compared with those obtained through more conventional fat-processing methods, i...
September 14, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28904532/the-use-of-adipose-derived-stem-cells-in-selected-skin-diseases-vitiligo-alopecia-and-nonhealing-wounds
#7
REVIEW
Agnieszka Owczarczyk-Saczonek, Anna Wociór, Waldemar Placek, Wojciech Maksymowicz, Joanna Wojtkiewicz
The promising results derived from the use of adipose-derived stem cells (ADSCs) in many diseases are a subject of observation in preclinical studies. ADSCs seem to be the ideal cell population for the use in regenerative medicine due to their easy isolation, nonimmunogenic properties, multipotential nature, possibilities for differentiation into various cell lines, and potential for angiogenesis. This article reviews the current data on the use of ADSCs in the treatment of vitiligo, various types of hair loss, and the healing of chronic wounds...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28894288/altered-adipose-derived-stem-cell-characteristics-in-macrodactyly
#8
Xi Yang, Yongkang Jiang, Gang Han, Yuan Shi, Shengbo Zhou, Feng Ni, Bin Wang
Macrodactyly is a congenital disease characterized by aggressive overgrowth of adipose tissue in digits or limbs frequently accompanied with hyperostosis and nerve enlargement; its pathological mechanism is poorly understood. Adipose-derived stem cells (ASCs) have been extensively studied in tissue engineering and regenerative medicine as an ideal alternative substitute for bone marrow-derived mesenchymal stem cells (BM-MSCs), but their pathological role is largely unknown. In this study, ASCs from macrodactyly adipose tissues (Mac-ASCs) were isolated and compared to ASCs derived from the normal abdominal subcutaneous adipose tissue (Sat-ASCs) for cell morphology, surface marker expression, proliferation rate, and tri-lineage differentiation potential...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28893630/engineered-axially-vascularized-osteogenic-grafts-from-human-adipose-derived-cells-to-treat-avascular-necrosis-of-bone-in-a-rat-model
#9
Tarek Ismail, Rik Osinga, Atanas Todorov, Alexander Haumer, Laurent A Tchang, Christian Epple, Nima Allafi, Nadia Menzi, René D Largo, Alexandre Kaempfen, Ivan Martin, Dirk J Schaefer, Arnaud Scherberich
BACKGROUND: Avascular necrosis of bone (AVN) leads to sclerosis and collapse of bone and joints. The standard of care, vascularized bone grafts, is limited by donor site morbidity and restricted availability. The aim of this study was to generate and test engineered, axially vascularized SVF cells-based bone substitutes in a rat model of AVN. METHODS: SVF cells were isolated from lipoaspirates and cultured onto porous hydroxyapatite scaffolds within a perfusion-based bioreactor system for 5 days...
September 8, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28891216/the-cd45-fraction-in-murine-adipose-tissue-derived-stromal-cells-harbor-immune-inhibitory-inflammatory-cells
#10
Alessandro Nasti, Yoshio Sakai, Akihiro Seki, Geraldine Belen Buffa, Takuya Komura, Hatsune Mochida, Masatoshi Yamato, Keiko Yoshida, Tuyen T B Ho, Masayuki Takamura, Soichiro Usui, Takashi Wada, Masao Honda, Shuichi Kaneko
Stromal cells in adipose tissue are useful for repair/regenerative therapy as they harbor a substantial number of mesenchymal stem cells; therefore, freshly isolated autologous uncultured adipose tissue-derived stromal cells (u-ADSCs) are useful for regenerative therapy, and obviate the need for mesenchymal stem cells. We evaluated the therapeutic effect of murine u-ADSCs and sorted subsets of u-ADSCs in a concanavalin A (ConA)-induced murine model of hepatitis, as well as their characteristics. We found that 10% to 20% of u-ADSCs expressed the CD45 leukocyte-related antigen...
September 11, 2017: European Journal of Immunology
https://www.readbyqxmd.com/read/28888046/gap-junctional-intercellular-communication-in-adipose-derived-stromal-stem-cells-is-cell-density-dependent-and-positively-impacts-adipogenic-differentiation
#11
Wiesner Miriam, Berberich Oliver, Hoefner Christiane, Blunk Torsten, Bauer-Kreisel Petra
Adipose-derived stromal/stem cells (ASCs) represent a widely used cell source with multi-lineage differentiation capacity in approaches for tissue engineering and regenerative medicine. Despite the multitude of literature on their differentiation capacity, little is reported about the physiological properties contributing to and controlling the process of lineage differentiation. Direct intercellular communication between adjacent cells via gap junctions has been shown to modulate differentiation processes in other cell types, with connexin 43 (Cx43) being the most abundant isoform of the gap junction-forming connexins...
September 9, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28872452/retraction
#12
(no author information available yet)
Since the following case report was found not to meet the ethical standards of Juntendo University, as previously stated by the authors, the article has been retracted from Regenerative Medicine: Kantake M, Hirano A, Sano M, Urushihata N, Tanemura H, Oki K, Suzaki E. Transplantation of allogeneic adipose-derived mesenchymal stem cells in a cerebral palsy patient. Regenerative Medicine doi:10.2217/rme-2017-0043 (2017) (Epub ahead of print). The authors and editors of Regenerative Medicine regret any negative consequences this publication might have caused in the scientific and medical communities...
September 5, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28872416/retraction
#13
(no author information available yet)
Since the following case report was found not to meet the ethical standards of Juntendo University, as previously stated by the authors, the article has been retracted from Regenerative Medicine: Kantake M, Hirano A, Sano M, Urushihata N, Tanemura H, Oki K, Suzaki E. Transplantation of allogeneic adipose-derived mesenchymal stem cells in a cerebral palsy patient. Regenerative Medicine doi:10.2217/rme-2017-0043 (2017) (Epub ahead of print). The authors and editors of Regenerative Medicine regret any negative consequences this publication might have caused in the scientific and medical communities...
July 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28869459/modulation-of-the-mesenchymal-stem-cell-migration-capacity-via-preconditioning-with-topographic-microstructure
#14
Zhengdong Li, Xun Xu, Weiwei Wang, Karl Kratz, Xianlei Sun, Jie Zou, Zijun Deng, Friedrich Jung, Manfred Gossen, Nan Ma, Andreas Lendlein
Controlling mesenchymal stem cells (MSCs) behavior is necessary to fully exploit their therapeutic potential. Various approaches are employed to effectively influence the migration capacity of MSCs. Here, topographic microstructures with different microscale roughness were created on polystyrene (PS) culture vessel surfaces as a feasible physical preconditioning strategy to modulate MSC migration. By analyzing trajectories of cells migrating after reseeding, we demonstrated that mobilization velocity of human adipose derived mesenchymal stem cells (hADSCs) could be promoted and persisted by brief preconditioning with the appropriate microtopography...
August 28, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28855688/maximizing-non-enzymatic-methods-for-harvesting-adipose-derived-stem-from-lipoaspirate-technical-considerations-and-clinical-implications-for-regenerative-surgery
#15
Barbara Bellei, Emilia Migliano, Marinella Tedesco, Silvia Caputo, Mauro Picardo
In the past decade, adipose tissue has become a highly interesting source of adult stem cells for plastic surgery and regenerative medicine. The adipose source offers two options for the isolation of regenerative cells: the enzymatic digestion an expensive time-consuming procedure lacking a common standard operating protocol, or the non-enzymatic dissociation methods based on mechanical forces to break the processed adipose tissue. Here, we propose innovative inexpensive non-enzymatic protocols to collect and concentrate clinically useful regenerative cells from adipose tissue by centrifugation of the infranatant fraction of lipoaspirate as first step, usually discarded as a byproduct of the surgical procedure, and by fat shaking and wash as second enrichment step...
August 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28851519/bioactive-glass-ceramic-nanoparticles-coated-poly-l-lactic-acid-scaffold-improved-osteogenic-differentiation-of-adipose-stem-cells-in-equine
#16
Fatemeh Sadat Mahdavi, Abdolreza Salehi, Ehsan Seyedjafari, Abdollah Mohammadi-Sangcheshmeh, Abdolreza Ardeshirylajimi
Horses with big bone fractures have low chance to live mainly due to the lake of a proper treatment strategy. We believe that further attempts in equine bone tissue engineering will probably be required to meet all the needs for the lesion therapies. Therefore in this study we aimed to investigate the osteogenic differentiation capacity of equine adipose-derived stem cells (e-ASCs) on nano-bioactive glass (nBGs) coated poly(l-lactic acid) (PLLA) nanofibers scaffold (nBG-PLLA). Using electrospinning technique, PLLA scaffold was prepared successfully and coated with nBGs...
July 20, 2017: Tissue & Cell
https://www.readbyqxmd.com/read/28843066/deriving-vascular-smooth-muscle-cells-from-mesenchymal-stromal-cells-evolving-differentiation-strategies-and-current-understanding-of-their-mechanisms
#17
REVIEW
Xiaoqing Zhang, Michelle P Bendeck, Craig A Simmons, J Paul Santerre
Vascular smooth muscle cells (VSMCs) play essential roles in regulating blood vessel form and function. Regeneration of functional vascular smooth muscle tissue to repair vascular diseases is an area of intense research in tissue engineering and regenerative medicine. For functional vascular smooth muscle tissue regeneration to become a practical therapy over the next decade, the field will need to have access to VSMC sources that are effective, robust and safe. While pluripotent stem cells hold good future promise to this end, more immediate translation is expected to come from approaches that generate functional VSMCs from adult sources of multipotent adipose-derived and bone marrow-derived mesenchymal stromal cells (ASCs and BMSCs)...
August 15, 2017: Biomaterials
https://www.readbyqxmd.com/read/28841158/mesenchymal-stem-cell-secretome-toward-cell-free-therapeutic-strategies-in-regenerative-medicine
#18
REVIEW
Francisco J Vizoso, Noemi Eiro, Sandra Cid, Jose Schneider, Roman Perez-Fernandez
Earlier research primarily attributed the effects of mesenchymal stem cell (MSC) therapies to their capacity for local engrafting and differentiating into multiple tissue types. However, recent studies have revealed that implanted cells do not survive for long, and that the benefits of MSC therapy could be due to the vast array of bioactive factors they produce, which play an important role in the regulation of key biologic processes. Secretome derivatives, such as conditioned media or exosomes, may present considerable advantages over cells for manufacturing, storage, handling, product shelf life and their potential as a ready-to-go biologic product...
August 25, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28836202/isolation-and-culture-of-adipose-derived-stromal-cells-from-subcutaneous-fat
#19
Lawrence J Liew, Huan Ting Ong, Rodney J Dilley
Cells with mesenchymal stem cell characteristics can be isolated from human adipose tissue stroma. Relative ease of isolation in large numbers and their ability for expansion and differentiation means that they are becoming a preferred cell type for mesenchymal regenerative medicine applications. In addition to expansion and differentiation capacity, they also express valuable paracrine activities which promote tissue formation and wound healing, including pro- and anti-fibrotic mediators. Here we describe a method for routine isolation of adipose stromal cells, culture expansion, and characterization by differentiation and then production of conditioned media...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28834402/concise-review-fetal-membranes-in-regenerative-medicine-new-tricks-from-an-old-dog
#20
REVIEW
Rebecca Lim
The clinical application of the fetal membranes dates back to nearly a century. Their use has ranged from superficial skin dressings to surgical wound closure. The applications of the fetal membranes are constantly evolving, and key to this is the uncovering of multiple populations of stem and stem-like cells, each with unique properties that can be exploited for regenerative medicine. In addition to pro-angiogenic and immunomodulatory properties of the stem and stem-like cells arising from the fetal membranes, the dehydrated and/or decellularized forms of the fetal membranes have been used to support the growth and function of other cells and tissues, including adipose-derived mesenchymal stem cells...
September 2017: Stem Cells Translational Medicine
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