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Adipose derived regenerative cells

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https://www.readbyqxmd.com/read/27920882/paroxetine-can-enhance-neurogenesis-during-neurogenic-differentiation-of-human-adipose-derived-stem-cells
#1
Maliheh Jahromi, Shahnaz Razavi, Nushin Amirpour, Zahra Khosravizadeh
BACKGROUND: Some antidepressant drugs can promote neuronal cell proliferation in vitro as well as hippocampal neurogenesis in human and animal models. Furthermore, adipose tissue is an available source of adult stem cells with the ability to differentiate in to multiple lineages. Therefore, human Adipose-Derived Stem Cells (hAD-SCs) may be a suitable source for regenerative medical applications. Since there is no evidence for the effect of Paroxetine as the most commonly prescribed antidepressant drug for neurogenic potential of hADSCs, an attempt was made to determine the effect of Paroxetine on proliferation and neural differentiation of hADSCs...
October 2016: Avicenna Journal of Medical Biotechnology
https://www.readbyqxmd.com/read/27893630/a-comprehensive-in-vitro-comparison-of-preparation-techniques-for-fat-grafting
#2
Libor Streit, Josef Jaros, Veronika Sedlakova, Miroslava Sedlackova, Lubos Drazan, Michal Svoboda, Jakub Pospisil, Tomas Vyska, Jiri Vesely, Ales Hampl
BACKGROUND: Lipomodelling is a technique that uses patient's own fat for tissue regeneration and augmentation. The extent of regenerative effect is reported to be determined by the numbers of adipose tissue-derived stem cells (ASCs) and the viability of cells in processed adipose tissue, which, together with other factors, influence the degree of graft retention. This study addresses whether differences exist in properties of fat graft obtained by three commonly used techniques. METHODS: Adipose tissue harvested from the hypo-gastric regions of 14 patients was processed by decantation, centrifugation, and membrane-based tissue filtration...
November 21, 2016: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/27883074/adipose-stem-cells-display-higher-regenerative-capacities-and-more-adaptable-electro-kinetic-properties-compared-to-bone-marrow-derived-mesenchymal-stromal-cells
#3
Ahmed El-Badawy, Marwa Amer, Reda Abdelbaset, Sameh N Sherif, Marwan Abo-Elela, Yehya H Ghallab, Hamdy Abdelhamid, Yehea Ismail, Nagwa El-Badri
Adipose stem cells (ASCs) have recently emerged as a more viable source for clinical applications, compared to bone-marrow mesenchymal stromal cells (BM-MSCs) because of their abundance and easy access. In this study we evaluated the regenerative potency of ASCs compared to BM-MSCs. Furthermore, we compared the dielectric and electro-kinetic properties of both types of cells using a novel Dielectrophoresis (DEP) microfluidic platform based on a printed circuit board (PCB) technology. Our data show that ASCs were more effective than BM-MSCs in promoting neovascularization in an animal model of hind-limb ischemia...
November 24, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27869309/roles-of-notch-signaling-in-adipocyte-progenitor-cells-and-mature-adipocytes
#4
REVIEW
Tizhong Shan, Jiaqi Liu, Weiche Wu, Ziye Xu, Yizhen Wang
Adipose tissues, composed with mature adipocytes and preadipocytic stromal/stem cells, play crucial roles in whole body energy metabolism and regenerative medicine. Mature adipocytes are derived and differentiated from mesenchymal stem cells (MSCs) or preadipocytes. This differentiation process, also called adipogenesis, is regulated by several signaling pathways and transcription factors. Notch1 signaling is a highly conserved pathway that is indispensable for stem cell hemostasis and tissue development. In adipocyte progenitor cells, Notch1 signaling regulates the adipogenesis process including proliferation and differentiation of the adipocyte progenitor cells in vitro...
November 21, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27865937/lipopolysaccharide-induces-proliferation-and-osteogenic-differentiation-of-adipose-derived-mesenchymal-stromal-cells-in-vitro-via-tlr4-activation
#5
Nicole Herzmann, Achim Salamon, Tomas Fiedler, Kirsten Peters
Multipotent mesenchymal stromal cells (MSC) are capable of multi-lineage differentiation and support regenerative processes. In bacterial infections, resident MSC can come intocontact with and need to react to bacterial components. Lipopolysaccharide (LPS), a typical structure of Gram-negative bacteria, increases the proliferation and osteogenic differentiation of MSC. LPS is usually recognized by the toll-like receptor (TLR) 4 and induces pro-inflammatory reactions in numerous cell types. In this study, we quantified the protein expression of TLR4 and CD14 on adipose-derived MSC (adMSC) in osteogenic differentiation and investigated the effect of TLR4 activation by LPS on NF-κB activation, proliferation and osteogenic differentiation of adMSC...
November 16, 2016: Experimental Cell Research
https://www.readbyqxmd.com/read/27855539/mouse-p2y4-nucleotide-receptor-is-a-negative-regulator-of-cardiac-adipose-derived-stem-cell-differentiation-and-cardiac-fat-formation
#6
Anne Lemaire, Marion Vanorlé, Michael Horckmans, Larissa di Pietrantonio, Sophie Clouet, Bernard Robaye, Jean-Marie Boeynaems, Didier Communi
Cardiac adipose-derived stem cells (cASCs) have the ability to differentiate into multiple cell lineages giving them a high potential for use in regenerative medicine. Cardiac fat tissue still raises many unsolved questions related to its formation and features. P2Y nucleotide receptors have already been described as regulators of differentiation of bone-marrow derived stem cells but remain poorly investigated in cASCs. We defined here the P2Y4 nucleotide receptor as a negative regulator of cardiac fat formation and cASC differentiation...
November 17, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27855196/age-related-changes-in-the-regenerative-potential-of-adipose-derived-stem-cells-isolated-from-the-prominent-fat-pads-in-human-lower-eyelids
#7
Xinhai Ye, Caihe Liao, Guangpeng Liu, Yipin Xu, Jian Tan, Zhenshun Song
The existence of multipotent adipose-derived stem cells isolated from human orbital fat (OF) tissue has shown great therapeutic potential in tissue engineering and regenerative medicine. But the use of stem cells for therapeutic applications is influenced by their proliferative and differentiation potentials, which may be affected by the age of the donor. So far there is little knowledge about the effects of donor age on the biological properties of human orbital adipose-derived stem cells (OASCs). The intraorbital fat protrusion in the lower eyelids occurs as an aging process, and the protruded fat is routinely removed during aesthetic surgeries...
2016: PloS One
https://www.readbyqxmd.com/read/27852267/culture-expansion-of-adipose-derived-stromal-cells-a-closed-automated-quantum-cell-expansion-system-compared-with-manual-flask-based-culture
#8
Mandana Haack-Sørensen, Bjarke Follin, Morten Juhl, Sonja K Brorsen, Rebekka H Søndergaard, Jens Kastrup, Annette Ekblond
BACKGROUND: Adipose derived stromal cells (ASCs) are a rich and convenient source of cells for clinical regenerative therapeutic approaches. However, applications of ASCs often require cell expansion to reach the needed dose. In this study, cultivation of ASCs from stromal vascular fraction (SVF) over two passages in the automated and functionally closed Quantum Cell Expansion System (Quantum system) is compared with traditional manual cultivation. METHODS: Stromal vascular fraction was isolated from abdominal fat, suspended in α-MEM supplemented with 10% Fetal Bovine Serum and seeded into either T75 flasks or a Quantum system that had been coated with cryoprecipitate...
November 16, 2016: Journal of Translational Medicine
https://www.readbyqxmd.com/read/27837561/banking-of-adipose-and-cord-tissue-derived-stem-cells-technical-and-regulatory-issues
#9
David T Harris
Stem cells are found in all multicellular organisms and are defined as cells that can differentiate into specialized mature cells as well as divide to produce more stem cells. Mesenchymal stem cells (MSC) were among the first stem cell types to be utilized for regenerative medicine. Although initially isolated from bone marrow, based on ease and costs of procurement, MSC derived from adipose tissue (AT-MSC) and umbilical cord tissue (CT-MSC) are now preferred stem cell sources for these applications. Both adipose tissues and cord tissue present unique problems for biobanking however, in that these are whole tissues, not cellular suspensions...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27837560/cryopreserved-adipose-tissue-derived-stromal-stem-cells-potential-for-applications-in-clinic-and-therapy
#10
Fabiana Zanata, Shahensha Shaik, Ram V Devireddy, Xiying Wu, Lydia Masako Ferreira, Jeffrey M Gimble
Adipose-Derived Stromal/Stem Cells (ASC) have considerable potential for regenerative medicine due to their abilities to proliferate, differentiate into multiple cell lineages, high cell yield, relative ease of acquisition, and almost no ethical concerns since they are derived from adult tissue. Storage of ASC by cryopreservation has been well described that maintains high cell yield and viability, stable immunophenotype, and robust differentiation potential post-thaw. This ability is crucial for banking research and for clinical therapeutic purposes that avoid the morbidity related to repetitive liposuction tissue harvests...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27830734/vegf-released-by-deferoxamine-preconditioned-mesenchymal-stem-cells-seeded-on-collagen-gag-substrates-enhances-neovascularization
#11
Elizabeth A Wahl, Thilo L Schenck, Hans-Günther Machens, Elizabeth R Balmayor
Hypoxia preconditioning of mesenchymal stem cells (MSCs) has been shown to promote wound healing through HIF-1α stabilization. Preconditioned MSCs can be applied to three-dimensional biomaterials to further enhance the regenerative properties. While environmentally induced hypoxia has proven difficult in clinical settings, this study compares the wound healing capabilities of adipose derived (Ad) MSCs seeded on a collagen-glycosaminoglycan (GAG) dermal substrate exposed to either environmental hypoxia or FDA approved deferoxamine mesylate (DFO) to stabilize HIF-1α for wound healing...
November 10, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27798372/safety-studies-for-use-of-adipose-tissue-derived-mesenchymal-stromal-stem-cells-in-a-rabbit-model-for-osteoarthritis-to-support-a-phase-i-clinical-trial
#12
Scott M Riester, Janet M Denbeigh, Yang Lin, Dakota L Jones, Tristan de Mooij, Eric A Lewallen, Hai Nie, Christopher R Paradise, Darcie J Radel, Amel Dudakovic, Emily T Camilleri, Dirk R Larson, Wenchun Qu, Aaron J Krych, Matthew A Frick, Hee-Jeong Im, Allan B Dietz, Jay Smith, Andre J van Wijnen
: : Adipose-derived mesenchymal stem cells (AMSCs) offer potential as a therapeutic option for clinical applications in musculoskeletal regenerative medicine because of their immunomodulatory functions and capacity for trilineage differentiation. In preparation for a phase I clinical trial using AMSCs to treat patients with osteoarthritis, we carried out preclinical studies to assess the safety of human AMSCs within the intra-articular joint space. Culture-expanded human AMSCs grown in human platelet-lysate were delivered via intra-articular injections into normal healthy rabbit knees and knees at risk for the development of osteoarthritis after bilateral medial anterior hemimeniscectomy...
October 26, 2016: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/27789947/effects-of-nanoporous-anodic-titanium-oxide-on-human-adipose-derived-stem-cells
#13
Katarzyna Malec, Joanna Góralska, Magdalena Hubalewska-Mazgaj, Paulina Głowacz, Magdalena Jarosz, Pawel Brzewski, Grzegorz D Sulka, Marian Jaskuła, Iwona Wybrańska
The aim of current bone biomaterials research is to design implants that induce controlled, guided, successful, and rapid healing. Titanium implants are widely used in dental, orthopedic, and reconstructive surgery. A series of studies has indicated that cells can respond not only to the chemical properties of the biomaterial, but also, in particular, to the changes in surface topography. Nanoporous materials remain in focus of scientific queries due to their exclusive properties and broad applications. One such material is nanostructured titanium oxide with highly ordered, mutually perpendicular nanopores...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27788905/biocellular-regenerative-medicine-use-of-adipose-derived-stem-stromal-cells-and-it-s-native-bioactive-matrix
#14
REVIEW
Robert W Alexander
"Using your own tissues to heal" represents a major health care paradigm change and is one of the most exciting minimally invasive options currently available. Biocellular regenerative therapies are rapidly improving in documentation and cellular analyses and are gaining good safety and efficacy profiles. Once considered purely experimental, they have entered into an accepted, translational period to clinical providers, backed by improving science supporting the basic hypotheses. It is a well-recognized and reported alternative to many traditional medical/surgical interventions...
November 2016: Physical Medicine and Rehabilitation Clinics of North America
https://www.readbyqxmd.com/read/27761667/co2-laser-increases-the-regenerative-capacity-of-human-adipose-derived-stem-cells-by-a-mechanism-involving-the-redox-state-and-enhanced-secretion-of-pro-angiogenic-molecules
#15
Alina Constantin, Madalina Dumitrescu, Maria Cristina Mihai Corotchi, Dana Jianu, Maya Simionescu
CO2 laser has a beneficial effect on stem cells by mechanisms that are not clearly elucidated. We hypothesize that the effect of fractional CO2 laser on human adipose-derived stem cells (ADSC) could be due to changes in redox homeostasis and secretion of factors contributing to cellular proliferation and angiogenic potential. ADSC incubated in medium containing 0.5 or 10 % FBS were exposed to a single irradiation of a 10,600-nm fractional CO2 laser; non-irradiated ADSC were used as control. Viability/proliferation of ADSC was assessed by MTT assay; the intracellular reactive oxygen species (ROS) levels and the mitochondrial membrane potential (∆Ψm) were determined with DCFH-DA and JC-1 fluorescent probes, respectively...
October 20, 2016: Lasers in Medical Science
https://www.readbyqxmd.com/read/27760382/regeneration-patterns-influence-hindlimb-automutilation-after-sciatic-nerve-repair-using-stem-cells-in-rats
#16
Daniel Haselbach, Wassim Raffoul, Lorenz Larcher, Mathias Tremp, Daniel F Kalbermatten, Pietro G di Summa
INTRODUCTION: Hindlimb autophagy is common after rat sciatic total axotomy and is considered as a sign of neuropathic pain. We applied adult stem cells in a fibrin conduit in a total sciatic axotomy model to improve nerve regeneration, investigating whether a correlation could be detected between stem cells effects on regeneration and limb autophagy. MATERIAL AND METHODS: After sciatic nerve section, a 1-cm sciatic gap was crossed using fibrin conduits. Experimental groups included empty fibrin conduits, fibrin conduits seeded with primary Schwann cells, and fibrin conduits seeded with Schwann cell-like differentiated mesenchymal or adipose-derived stem cells (dMSCs and dASCs)...
October 17, 2016: Neuroscience Letters
https://www.readbyqxmd.com/read/27757364/microcirculatory-response-in-vivo-on-local-intraarterial-infusion-of-autogenic-adipose-derived-stem-cells-or-stromal-vascular-fraction
#17
Wei Z Wang
: Both adipose-derived stem cells (ASCs) and stromal vascular fraction (SVF) have been demonstrated to have regenerative properties with therapeutic potential for numerous diseases through local or topical applications. However, it is unclear whether ASC or SVF can be delivered systemically through an intra-arterial infusion. The purpose of this study was to examine the microcirculatory response in vivo on local intraarterial infusion of autogenic ASCs or SVF in a vascular pedicle isolated rat cremaster microcirculation model...
September 2016: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/27756443/human-adipose-derived-mesenchymal-stem-cell-spheroids-improve-recovery-in-a-mouse-model-of-elastase-induced-emphysema
#18
RyeonJin Cho, You-Sun Kim, Ji-Young Kim, Yeon-Mok Oh
BACKGROUND/PURPOSE: Emphysema, a pathologic component of chronic obstructive pulmonary disease, causes irreversible destruction. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. METHODS: We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in elastase-induced emphysema mice. ASCs were administered via an intrapleural route. RESULTS: Mice injected with spheroid ASCs showed improved regeneration of lung tissue, increased expression of growth factors such as FGF2 and HGF, and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs...
October 19, 2016: BMB Reports
https://www.readbyqxmd.com/read/27733784/evaluation-of-adipose-derived-stromal-vascular-fraction-from-the-lateral-tailhead-inguinal-region-and-mesentery-of-horses
#19
Garrett L Metcalf, Scott R McClure, Jesse M Hostetter, Rudy F Martinez, Chong Wang
Use of mesenchymal stem cells (MSCs) found in the stromal vascular fraction (SVF) of equine adipose tissue has promising applications for regenerative therapies. The most commonly used source of equine adipose tissue is the subcutaneous tailhead. The objective of this study was to compare 3 adipose depot sites in horses and determine the viability and cellular yield, capillary density, gene expression for selected markers, and colony-forming unit fibroblasts (CFU-Fs) in adipose tissue taken from these sites...
October 2016: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
https://www.readbyqxmd.com/read/27733094/cell-co-transplantation-strategies-for-vascularized-craniofacial-bone-tissue-engineering-a-systematic-review-and-meta-analysis-of-pre-clinical-in-vivo-studies
#20
Siddharth Shanbhag, Kamal Mustafa, Nikolaos Pandis, Jens R Nyengaard, Andreas Stavropoulos
The regenerative potential of tissue engineered bone constructs may be enhanced by in vitro co-culture and in vivo co-transplantation of vasculogenic and osteogenic (progenitor) cells. The objective of this study was to systematically review the literature to answer the focused question: in animal models, does co-transplantation of osteogenic and vasculogenic cells enhance bone regeneration in craniofacial defects, compared to solely osteogenic cell-seeded constructs? Following PRISMA guidelines, electronic databases were searched for controlled animal studies reporting co-transplantation of endothelial cells (EC) with mesenchymal stem cells (MSC) or osteoblasts (OB) in craniofacial critical-size bone defect (CSD) models...
October 12, 2016: Tissue Engineering. Part B, Reviews
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