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Adipose derived regenerative cells

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https://www.readbyqxmd.com/read/29776537/comparative-study-on-metabolite-level-in-tissue-specific-human-mesenchymal-stem-cells-by-an-ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#1
Seul Ji Lee, TacGhee Yi, Soo Hyun Ahn, Dong Kyu Lim, Si-Na Kim, Hyun-Joo Lee, Yun-Kyoung Cho, Jae-Yol Lim, Jong-Hyuk Sung, Jeong-Ho Yun, Johan Lim, Sun U Song, Sung Won Kwon
Mesenchymal stem cells (MSCs) are a promising therapeutic option for cell-based therapy due to their immunomodulatory and regenerative properties. They can be isolated from various adult tissues, including bone marrow, fat, dental tissue, and glandular tissue. Although they share common characteristics, little is known about the biological differences between MSC populations derived from different tissues. In this study, we used MS to compare the endogenous metabolite level in the human MSCs originating from the bone marrow, adipose tissue, periodontal ligaments, and salivary glands...
September 18, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29760738/dental-mesenchymal-stem-stromal-cells-and-their-exosomes
#2
REVIEW
Peter Stanko, Ursula Altanerova, Jana Jakubechova, Vanda Repiska, Cestmir Altaner
Stem cells derived from human dental pulp tissue (DP-MSC) differ from the other mesenchymal stem cells prepared from bone marrow or adipose tissue due to their embryonic origin from the neural crest and are of special interest because of their neurotropic character. Furthermore, the therapeutic potential of DP-MSCs is realized through paracrine action of extracellularly released components, for which exosomes play an important role. In this review, we intend to explore the properties of these cells with an emphasis on exosomes...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29760737/injection-laryngoplasty-using-autologous-fat-enriched-with-adipose-derived-regenerative-stem-cells-a-safe-therapeutic-option-for-the-functional-reconstruction-of-the-glottal-gap-after-unilateral-vocal-fold-paralysis
#3
José M Lasso, Daniel Poletti, Batolomé Scola, Pedro Gómez-Vilda, Ana I García-Martín, María Eugenia Fernández-Santos
Background: Paralysis of one vocal fold leads to glottal gap and vocal fold insufficiency that has significant impact upon a patient's quality of life. Fillers have been tested to perform intracordal injections, but they do not provide perdurable results. Early data suggest that enriching fat grafts with adipose-derived regenerative cells (ADRCs) promote angiogenesis and modulate the immune response, improving graft survival. The aim of this study is to propose ADRC-enriched adipose tissue grafts as effective filler for the paralyzed vocal fold to use it for functional reconstruction of the glottal gap...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29755530/the-effect-of-age-on-the-regenerative-potential-of-human-eyelid-adipose-derived-stem-cells
#4
Mingqi Zhang, Zhuoshi Wang, Yan Zhao, Lirong Zhang, Ling Xu, Liu Cao, Wei He
Human eyelid adipose-derived stem cells (HEASCs) are a new source of autologous mesenchymal stem cells, which are derived from neuroectoderm and potentially applied in the tissue regeneration and cell therapies. Based on the prevalence of blepharoplasty in Asia and the availability of HEASCs, we investigated the effect of donor age on their characteristics and regenerative potential of HEASCs in vitro. The HEASCs were isolated from patients of three groups: (1) <20 years ( n = 4), (2) >20 years, <45 years ( n = 5), and (3) >55 years ( n = 4)...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29751821/improved-gmp-compliant-approach-to-manipulate-lipoaspirates-to-cryopreserve-stromal-vascular-fraction-and-to-expand-adipose-stem-cells-in-xeno-free-media
#5
Francesco Agostini, Francesca Maria Rossi, Donatella Aldinucci, Monica Battiston, Elisabetta Lombardi, Stefania Zanolin, Samuele Massarut, Pier Camillo Parodi, Alessandro Da Ponte, Giovanni Tessitori, Barbara Pivetta, Cristina Durante, Mario Mazzucato
BACKGROUND: The stromal vascular fraction (SVF) derived from adipose tissue contains adipose-derived stromal/stem cells (ASC) and can be used for regenerative applications. Thus, a validated protocol for SVF isolation, freezing, and thawing is required to manage product administration. To comply with Good Manufacturing Practice (GMP), fetal bovine serum (FBS), used to expand ASC in vitro, could be replaced by growth factors from platelet concentrates. METHODS: Throughout each protocol, GMP-compliant reagents and devices were used...
May 11, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29747379/magnesium-deprivation-potentiates-human-mesenchymal-stem-cell-transcriptional-remodeling
#6
Azzurra Sargenti, Sara Castiglioni, Elena Olivi, Francesca Bianchi, Alessandra Cazzaniga, Giovanna Farruggia, Concettina Cappadone, Lucia Merolle, Emil Malucelli, Carlo Ventura, Jeanette A M Maier, Stefano Iotti
Magnesium plays a pivotal role in energy metabolism and in the control of cell growth. While magnesium deprivation clearly shapes the behavior of normal and neoplastic cells, little is known on the role of this element in cell differentiation. Here we show that magnesium deficiency increases the transcription of multipotency markers and tissue-specific transcription factors in human adipose-derived mesenchymal stem cells exposed to a mixture of natural molecules, i.e., hyaluronic, butyric and retinoid acids, which tunes differentiation...
May 9, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29743810/auricular-cartilage-regeneration-with-adipose-derived-stem-cells-in-rabbits
#7
Se-Joon Oh, Hee-Young Park, Kyung-Un Choi, Sung-Won Choi, Sung-Dong Kim, Soo-Keun Kong, Kyu-Sup Cho
Tissue engineering cell-based therapy using induced pluripotent stem cells and adipose-derived stem cells (ASCs) may be promising tools for therapeutic applications in tissue engineering because of their abundance, relatively easy harvesting, and high proliferation potential. The purpose of this study was to investigate whether ASCs can promote the auricular cartilage regeneration in the rabbit. In order to assess their differentiation ability, ASCs were injected into the midportion of a surgically created auricular cartilage defect in the rabbit...
2018: Mediators of Inflammation
https://www.readbyqxmd.com/read/29738484/human-macrophages-preferentially-infiltrate-the-superficial-adipose-tissue
#8
Giuseppe Cappellano, Evi M Morandi, Johannes Rainer, Philipp Grubwieser, Katharina Heinz, Dolores Wolfram, David Bernhard, Susanne Lobenwein, Gerhard Pierer, Christian Ploner
Human abdominal subcutaneous adipose tissue consists of two individual layers&mdash;the superficial adipose tissue (SAT) and deep adipose tissue (DAT)&mdash;separated by the Scarpa&rsquo;s fascia. The present study focuses on the analysis of morphological and immunological differences of primary adipocytes, adipose-derived stem cells (ASC), and tissue-infiltrating immune cells found in SAT and DAT. Adipocytes and stromal vascular fraction (SVF) cells were isolated from human SAT and DAT specimens and phenotypically characterized by in vitro assays...
May 8, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29732151/fetal-hepatocyte-derived-culture-medium-elicits-adipocyte-differentiation-to-bile-duct-cell-lineages-in-a-mouse-model
#9
Hisataka Ogawa, Masamitsu Konno, Koichi Kawamoto, Naohiro Nishida, Jun Koseki, Tsunekazu Mizushima, Taroh Satoh, Hidetoshi Eguchi, Yuichiro Doki, Masaki Mori, Hideshi Ishii
Fetal cells in the developmental stages function with a distinct mechanism in comparison to adult tissues, which may be a useful source for regenerative medicine in postnatal medicine; however, the precise molecular mechanism remains to be elucidated fully. The present study investigated murine fetal hepatocytes, which were cultured in vitro , and the supernatants were used for the culture with murine adipose tissue-derived cells. Notably, the results indicated that fetal hepatocyte-derived culture medium elicits the induction of differentiation of adipose tissue-derived cells to bile duct cell lineages, but not to hepatocyte lineages in mice...
May 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29722764/platelet-rich-plasma-and-mesenchymal-stem-cells-exciting-but-%C3%A2-are-we-there-yet
#10
Isabel Andia, Jose I Martin, Nicola Maffulli
Joint conditions incapacitate free movement driving to a sedentary lifestyle, a major risk factor for chronic diseases. Regenerative procedures, involving the use of mesenchymal stem/stromal cells along with platelet-rich plasma (PRP), can help patients with these conditions. We describe the main characteristics of cellular products (bone marrow concentrate, stromal vascular fraction of adipose tissue, and mesenchymal stem/stromal cells derived from these tissues), and the potential benefits of combination with PRP in 3 scenarios: PRP lysates used during laboratory cell expansion; PRP to prime cellular products or the host tissue before cell implantation; PRP used as a vehicle for cell transplantation and to provide trophic signals...
June 2018: Sports Medicine and Arthroscopy Review
https://www.readbyqxmd.com/read/29722292/angiogenic-potential-of-spheroids-from-umbilical-cord-and-adipose-derived-multipotent-mesenchymal-stromal-cells-within-fibrin-gel
#11
Anastasiya Gorkun, Anastasia Shpichka, Irina Zurina, Anastasia V Koroleva, Nastasia V Kosheleva, Denis Aleksandrovich Nikishin, Denis Butnaru, Peter S Timashev, Vadim S Repin, Irina Saburina
One of the essential goals in regenerative medicine is microvascularization which enables an effective blood supply within de novo constructed tissues and organs. In our study, we used two common multipotent mesenchymal stromal cell sources (subcutaneous adipose tissue and Wharton's jelly of the umbilical cord) where is a subpopulation of endothelial precursors. In the medium supplemented with VEGF, the 3D cultures of UC MMSCs and ADSCs promoted the endothelial cell differentiation. To evaluate their ability to form a capillary-like network, we encapsulated spheroids within non-modified and PEGylated fibrin hydrogels...
May 3, 2018: Biomedical Materials
https://www.readbyqxmd.com/read/29717634/notch-signaling-enhances-stemness-by-regulating-metabolic-pathways-through-modifying-p53-nf-%C3%AE%C2%BAb-and-hif-1%C3%AE
#12
Hiroyuki Moriyama, Mariko Moriyama, Toshiyuki Ozawa, Daisuke Tsuruta, Taro Iguchi, Satoshi Tamada, Tatsuya Nakatani, Koichi Nakagawa, Takao Hayakawa
Human adipose-derived stromal cells (hASCs) are attractive for regenerative medicine, but their limited in vitro lifespan limits their therapeutic applicability. Recent data demonstrate that hypoxia may benefit the ex vivo culture of stem cells. Such cells exhibit a high level of glycolytic metabolism under hypoxic conditions. However, the physiological role of glycolytic activation and its underlying regulatory mechanism are incompletely understood. We have shown that when activated under conditions of 5% O<sub>2</sub>, Notch signaling dramatically increases the rate of glycolysis, improves proliferation efficiency, prevents senescence, and maintains the multipotency of hASCs...
May 2, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29715649/allogeneic-mesenchymal-stem-cell-transplantation-in-healthy-equine-superficial-digital-flexor-tendon-a-study-of-the-local-inflammatory-response
#13
Jaqueline Souza Brandão, Marina Landim Alvarenga, João Pedro Hubbe Pfeifer, Vitor Hugo Dos Santos, Carlos Eduardo Fonseca-Alves, Mirian Rodrigues, Renée Laufer-Amorim, José Antonio Lucas Castillo, Ana Liz Garcia Alves
The superficial digital flexor tendon (SDFT) is a structure frequently affected by injuries in high-performance athletic horses, and there are limited therapeutic options. Regenerative medicine has evolved significantly in treating different illnesses. However, understanding the cellular behaviour during mesenchymal stem cell (MSC) transplantation in healthy tissues is not fully known yet. To address the inflammatory response induced by allogeneic MSC transplantation, this study evaluated the local inflammatory response after the application of allogeneic adipose tissue-derived mesenchymal stem cells (AT-MSCs) in the equine tendon compared to an autologous transplant and the control group...
March 21, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29710394/adipose-derived-stem-cells-reduce-fibrosis-and-promote-nerve-regeneration-in-rats
#14
Pietro G di Summa, Luigi Schiraldi, Mario Cherubino, Carlo M Oranges, Daniel F Kalbermatten, Wassim Raffoul, Srinivas Madduri
Peripheral nerve regeneration is critical and challenging in the adult humans. High level of collagen infiltration (i.e., scar tissue), in the niche of injury, impedes axonal regeneration and path finding. Unfortunately, studies focusing on the modulation of scar tissue in the nerves are scarce. To address part of this problem, we have evaluated the differentiated adipose derived stem cells (dASCs) for their anti-fibrotic and regenerative effects in a 10 mm nerve gap model in rats. Three different animal groups (n=5) were treated with fibrin nerve conduits (empty), or seeded with dASCs (F+dASCs) and autograft respectively...
April 30, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29705993/directing-adipose-derived-stem-cells-into-keratinocyte-like-cells-impact-of-medium-composition-and-culture-condition
#15
L Petry, S Kippenberger, M Meissner, J Kleemann, R Kaufmann, U M Rieger, S Wellenbrock, G Reichenbach, N Zöller, E Valesky
BACKGROUND: Adipose-derived stem cells (ASC) are known to transdifferentiate into a wide range of different cell species in vitro including along the epidermal lineage. This property makes them a promising tool for regenerative medicine in order to restore the epidermal barrier. OBJECTIVE: The present study is dedicated to identify in vitro conditions enabling transdifferentiation to a keratinocyte-like phenotype. Especially, the impact of different culture conditions (media compositions, 2D-, 3D-cultures) and extracellular matrix (ECM) molecules was evaluated...
April 28, 2018: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29699385/local-mesenchymal-stem-cell-therapy-in-experimentally-induced-colitis-in-the-rat
#16
Fernando de la Portilla, Yaiza Yuste, Sheila Pereira, Carolina Olano, Maria Victoria Maestre, Francisco Javier Padillo
Background: Multipotent mesenchymal stem cells (MSCs) have been used in inflammatory bowel diseases because of their immunomodulatory and regenerative properties. We investigated their local use in an experimental model of colitis in the rat. Materials and Methods: Colitis was induced into 20 Wistar rats with local TNBS instillation. Allogeneic stem cells were derived from rat adipose tissue and labeled with PKH2 linker dye with creation of a control and a second group treated by a local injection into the rectal wall of 2×10⁶ allogeneic adipose tissue-derived stem cells (ADSCs)...
April 30, 2018: International Journal of Stem Cells
https://www.readbyqxmd.com/read/29692732/adipose-derived-mesenchymal-stem-cell-application-combined-with-fibrin-matrix-promotes-structural-and-functional-recovery-following-spinal-cord-injury-in-rats
#17
Yana O Mukhamedshina, Elvira R Akhmetzyanova, Alexander A Kostennikov, Elena Y Zakirova, Luisa R Galieva, Ekaterina E Garanina, Alexander A Rogozin, Andrey P Kiassov, Albert A Rizvanov
The use of stem and progenitor cells to restore damaged organs and tissues, in particular, the central nervous system, is currently considered a most promising therapy in regenerative medicine. At the same time, another approach aimed at stimulating regeneration with the use of stem cells encapsulated into a biopolymer matrix and capable of creating a specific microenvironment for the implanted cells similar to the natural extracellular matrix is under active development. Here, we study effects of the application of adipose-derived mesenchymal stem cells (AD-MSCs) combined with a fibrin matrix on post-traumatic reactions in the spinal cord in rats...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29687389/isolation-of-murine-adipose-derived-stromal-stem-cells-using-an-explant-culture-method
#18
Jie Li, Hui Li, Weidong Tian
Adipose tissue provides valuable cell source for tissue engineering, regenerative medicine, and adipose tissue biology studies. The most widely used adipose-derived stromal/stem cells (ASCs) isolation protocol involves enzymatic digestion with collagenase. However, the yield of the method is poor even impossible to collect enough stromal vascular fraction (SVF) for expansion when the sample size is small, for instance only new born mice is available for cell culture. Here, we describe an efficient protocol for the isolation and expansion of ASCs using explant culture...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29687388/isolation-of-human-adipose-derived-stem-cells-from-lipoaspirates
#19
Jie Li, J Lowry Curley, Z Elizabeth Floyd, Xiying Wu, Yuan Di C Halvorsen, Jeffrey M Gimble
Adipose tissue is as an abundant and accessible source of stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Here, we describe methods from our own laboratory and the literature for the isolation and expansion of adipose-derived stem cells (ASCs). We present a large scale procedure suitable for processing >100 mL volumes of lipoaspirate tissue specimens by collagenase digestion and a related procedure suitable for processing adipose tissue aspirates without digestion...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29687384/adipose-derived-stromal-vascular-fraction-cells-and-platelet-rich-plasma-basic-and-clinical-implications-for-tissue-engineering-therapies-in-regenerative-surgery
#20
Pietro Gentile, Valerio Cervelli
Cell-based therapy and regenerative medicine offer a paradigm shift in regard to various diseases causing loss of substance or volume and tissue or organ damage. Recently, many authors have focused their attention on mesenchymal stem cells for their capacity to differentiate into many cell lineages. The most widely studied types are bone marrow mesenchymal stem cells and adipose derived stem cells (ADSCs), which display similar results. Based on the literature, we believe that the ADSCs offer advantages because of lower morbidity during the harvesting procedure...
2018: Methods in Molecular Biology
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