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Join infection

Jianhua Wang, Yang Lu, Hong Xia, Qingshui Yin
PURPOSE: To investigate a unique procedure of joined transoral and retropharyngeal high cervical approach (JTRC) without mandibulectomy for treating upper cervical neoplasm involving both C2 and C3. METHOD: A 23-year-old male patient had neoplasma involving C2 and C3 that caused pathologic fracture of C2 and bony destruction of C3. The neoplasm excision and cervical spine reconstruction were performed through JTRC approach without splitting up the mandible. In this approach, there were two surgery windows that could be applied in turns by closing or opening the mouth to gain an ideal exposure...
October 18, 2016: European Spine Journal
Ismail Soner Koltas, Fadime Eroglu
Blastocystis is a common and emerging parasite often seen in many studies conducted in urban population, with scanty reports on rural communities. However, little is known about the public health significance of Blastocystis infection. A total of 28 Blastocystis isolates from 17 (17/28, 60.71%) patients living in rural area and 11 (11/28, 39.29%) patients living in urban area were screened with seven kinds of sequenced-tagged site primers for identification of subtype. PCR products were sequenced with same combination of primers using the BigDye Terminator V 3...
October 7, 2016: Experimental Parasitology
Marc Heincelman, Nithin Karakala, Don C Rockey
Acute lymphoblastic leukemia (ALL) in adults is a relatively rare malignancy. The typical presentation includes signs and symptoms associated with bone marrow failure, including fevers, infections, fatigue, and excessive bruising. In this article, we report an unusual systemic presentation of ALL in a previously healthy 18-year-old man. He initially presented with several-day history of nausea and vomiting, 10-pound weight loss, and right upper quadrant abdominal pain with evidence of acute hepatocellular liver injury (elevations in aspartate aminotransferase/alanine aminotransferase) and elevation in serum creatinine...
July 2016: Journal of Investigative Medicine High Impact Case Reports
Shinjiro Kobayashi, Horoyuki Hoshino, Kouhei Segami, Satoshi Koizumi, Nobuyuki Ooike, Takehito Otsubo
The patient was a 56-year-old woman who had experienced epigastralgia and dorsal pain several times over the last 20 years. She was admitted for a diagnosis of acute cholecystitis, and severe intra- and extrahepatic bile duct dilatation with inner air density was noted. No papilla of Vater was present in the descending duodenum, and 2 small holes were present in the pyloric ring. Bile excretion from one of the small holes was observed under forward-viewing endoscope. It was considered that the pancreatic and bile ducts separately opened into the pyloric ring...
May 2016: Case Reports in Gastroenterology
Catherine Su Hui Teo, Remigiusz A Serwa, Peter O'Hare
We used pulse-labeling with the methionine analogue homopropargylglycine (HPG) to investigate spatiotemporal aspects of protein synthesis during herpes simplex virus (HSV) infection. In vivo incorporation of HPG enables subsequent selective coupling of fluorochrome-capture reagents to newly synthesised proteins. We demonstrate that HPG labeling had no effect on cell viability, on accumulation of test early or late viral proteins, or on overall virus yields. HPG pulse-labeling followed by SDS-PAGE analysis confirmed incorporation into newly synthesised proteins, while parallel processing by in situ cycloaddition revealed new insight into spatiotemporal aspects of protein localisation during infection...
October 2016: PLoS Pathogens
Youfang Gu, Xiaolong Wang, Cefan Zhou, Peiying Li, Qianming Xu, Changcheng Zhao, Wei Liu, Wenlong Xu
To assess Cryptosporidium infections among wild animals in a zoo located in Anhui province, we conducted an investigation on the fecal samples collected from 44 primates, 41 herbivores, 44 carnivores and omnivores, and 103 birds in the zoo with the use of Sheather's sugar flotation technique and modified acid-fast staining. Cryptosporidium oocysts were detected in the fecal samples from six primates, two herbivores, four carnivores and omnivores, and seven birds by using Sheather's sugar flotation technique; the prevalence of Cryptosporidium infection in primates, herbivores, carnivores and omnivores and birds was 13...
September 2016: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Alberto C Guardo, Patrick Tjok Joe, L Miralles, M E Bargalló, B Mothe, A Krasniqi, C Heirman, F García, Kris Thielemans, Christian Brander, Joeri L Aerts, Montserrat Plana
BACKGROUND: The development of a prophylactic vaccine against HIV-1 has so far not been successful. Therefore, attention has shifted more and more towards the development of novel therapeutic vaccines. Here, we evaluated a new mRNA based therapeutic vaccine against HIV-1 encoding activation signals (TriMix: CD40L+CD70+caTLRA4) combined with rationally selected antigenic sequences (HTI sequence: comprised of 16 joined fragments from Gag, Pol, Vif and Nef). METHODS: For this purpose, PBMC from HIV-1-infected individuals on cART, lymph node explants from non-infected humans and splenocytes from immunized mice were collected and several immune functions were measured...
September 24, 2016: AIDS
Federica Del Chierico, David Di Cave, Cristel Accardi, Maristella Santoro, Andrea Masotti, Rossella D'Alfonso, Federica Berrilli, Andrea Urbani, Lorenza Putignani
Over the years, the potential pathogenicity of Acanthamoeba for humans and animals has gained increasing attention from the scientific community. More than 24 species belong to this genus, however only some of them are causative agents of keratitis and encephalitis in humans. Due to technical difficulties in diagnosis, these infections are likely to be under-detected. The introduction of 18S rDNA amplification for the identification of Acanthamoeba has dramatically enhanced diagnosis performances, but the attestation of genotyping requires supplementary sequencing-based procedures...
September 20, 2016: Experimental Parasitology
Hyeong-Kyu Jeon, Hansol Park, Dongmin Lee, Seongjun Choe, Woon-Mok Sohn, Keeseon S Eom
The genus Spirometra belongs to the family Diphyllobothriidae and order Pseudophyllidea, and includes intestinal parasites of cats and dogs. In this study, a plerocercoid labeled as Spirometra mansonoides from the USA was examined for species identification and phylogenetic analysis using 2 complete mitochondrial genes, cytochrome c oxidase I (cox1) and NADH dehydrogenase subunit 3 (nad3). The cox1 sequences (1,566 bp) of the plerocercoid specimen (USA) showed 99.2% similarity to the reference sequences of the plerocercoid of Korean Spirometra decipiens (GenBank no...
August 2016: Korean Journal of Parasitology
Attakorn Palasuwan, Duangdao Palasuwan, Aongart Mahittikorn, Rachatawan Chiabchalard, Valery Combes, Supaluk Popruk
Blastocystis is a common zoonotic enteric protozoan that has been classified into 17 distinct subtypes (STs). A cross-sectional study was conducted to determine the prevalence and subtype distributions of Blastocystis in villagers living along the Chao Phraya River, Ayutthaya Province, Thailand, and to assess the risk of zoonotic infection. In total, 220 stool samples were collected, and DNA was extracted. PCR and sequencing were performed with primers targeting the small-subunit ribosomal RNA (SSU rRNA) genes...
August 2016: Korean Journal of Parasitology
Suellen A Zatti, Sarah Arana, Antônio A M Maia, Edson A Adriano
Myxobolus prochilodus and Myxobolus porofilus are parasites of Prochilodus lineatus, an economically important South American fish found in La Plata and Paraiba do Sul river basins. This study focusing on parasite-host interaction provides an ultrastructural and phylogenetic analysis, the latter based on ssrDNA sequencing of these parasites respectively infecting the gill filaments and fins of P. lineatus taken from the Mogi Guaçu River, São Paulo, Brazil. A total of 13 adult specimens were examined in this study...
September 14, 2016: Parasitology Research
Caroline Bouter, Isabell Braune, Birgit Meller, Carsten Sahlmann, Carsten Ritter, Johannes Meller
: The diagnostic strategy in patients with fever or inflammation of unknown origin remains a major clinical challenge. The aim of this study was to evaluate the role of 18F-FDG-PET/CT in patients with unexplained elevated C-reactive protein with or without fever. Contribution of 18F-FDG-PET/CT to the final diagnosis was evaluated. In addition we determined whether a differentiation between patients with or without fever is clinically reasonable. PATIENTS, METHODS: We retrospectively analyzed 72 consecutive patients with unexplained elevated C-reactive protein levels (above 8mg/l) that underwent 18F-FDG-PET/CT in our facility between 10/2009 and 11/2012...
September 12, 2016: Nuklearmedizin. Nuclear Medicine
Douglas G Wright, Reneau Castore, Runhua Shi, Amrita Mallick, Don G Ennis, Lynn Harrison
Mycobacterium tuberculosis and Mycobacterium smegmatis express a Ku protein and a DNA ligase D and are able to repair DNA double strand breaks (DSBs) by non-homologous end-joining (NHEJ). This pathway protects against DNA damage when bacteria are in stationary phase. Mycobacterium marinum is a member of this mycobacterium family and like M. tuberculosis is pathogenic. M. marinum lives in water, forms biofilms and infects fish and frogs. M. marinum is a biosafety level 2 (BSL2) organism as it can infect humans, although infections are limited to the skin...
September 9, 2016: Mutagenesis
Julie A Thomas, Andrea Denisse Benítez Quintana, Martine A Bosch, Adriana Coll De Peña, Elizabeth Aguilera, Assitan Coulibaly, Weimin Wu, Michael V Osier, André O Hudson, Susan T Weintraub, Lindsay W Black
: Giant tailed bacterial viruses or phages, such as Pseudomonas aeruginosa phage φKZ, have long genomes packaged into large, atypical virions. Many aspects of φKZ and related phage biology are poorly understood mostly due to the fact that the functions of the majority of their proteins are unknown. We hypothesized that the Salmonella enterica phage SPN3US could be a useful model phage to address this gap in knowledge. The 240 kb SPN3US genome shares a core set of 91 genes with φKZ and related phages, of which ∼61 are virion genes, consistent with the expectation that virion complexity is an ancient, conserved feature...
September 7, 2016: Journal of Virology
Lindan Sun, Lizhu Yi, Chi Zhang, Xiaodan Liu, Shuangshuang Feng, Wenjie Chen, Jiangfeng Lan, Lijuan Zhao, Jiagang Tu, Li Lin
Snakehead fish vesiculovirus (SHVV), a member of the family Rhabdoviridae, has caused mass mortality to snakehead fish culture in China. Previous transcriptomic sequencing of SHVV-infected and non-infected striped snakehead fish cells (SSN-1) showed that glutaminase (GLS), the critical enzyme of glutamine metabolism, was up regulated upon SHVV infection. It therefore drew our attention to investigate the role of glutamine on SHVV propagation. Glutamine deprivation significantly reduced the expression of the mRNAs and proteins of SHVV, and the production of virus particles, indicating that glutamine was required for SHVV propagation...
September 6, 2016: Journal of General Virology
Dominik M Meinel, Richard Kuehl, Reinhard Zbinden, Veronika Boskova, Christian Garzoni, Davide Fadini, Marisa Dolina, Benjamin Blümel, Thomas Weibel, Sarah Tschudin-Sutter, Andreas F Widmer, Julia A Bielicki, Alexa Dierig, Ulrich Heininger, Regina Konrad, Anja Berger, Vladimira Hinic, Daniel Goldenberger, Annette Blaich, Tanja Stadler, Manuel Battegay, Andreas Sing, Adrian Egli
OBJECTIVES: Toxigenic Corynebacterium diphtheriae is an important and potential fatal threat to patients and public health. During the current dramatic influx of refugees in Europe, our objective was to use whole genome sequencing for the characterisation of a suspected outbreak of C. diphtheriae wound infections among refugees. METHODS: After conventional culture, we identified C. diphtheriae using MALDI-TOF mass-spectrometry and investigated toxigenicity by PCR...
August 29, 2016: Clinical Microbiology and Infection
Wei Zhen Chow, Abdul Hamid Bon, Sheila Keating, Fread Anderios, Hazwan Abdul Halim, Yutaka Takebe, Adeeba Kamarulzaman, Michael P Busch, Kok Keng Tee
Transfusion-transmissible infections including HIV-1 continue to pose major risks for unsafe blood transfusions due to both window phase infections and divergent viruses that may not be detected by donor screening assays. Given the recent emergence of several HIV-1 circulating recombinant forms (CRFs) in high-risk populations in the Southeast Asia region, we investigated the genetic diversity of HIV-1 among the blood donors in Kuala Lumpur, Malaysia. A total of 211 HIV-positive plasma samples detected among 730,188 donations to the National Blood Centre between 2013 and 2014 were provided (90...
2016: PloS One
Shuxian Li, Florent Bonneu, Joël Chadoeuf, Delphine Picart, Anne Gégout Petit, Lucia Guerin-Dubrana
To assess the capacity of esca to spread within vineyards of the Bordeaux region, over 8 years of annual records, containing between 1,200 and 2,300 contiguous Cabernet Sauvignon vines from 15 mature vineyards, were used for spatial statistical analyses. A group of non-parametric tests, based on join count statistics and on permutation methods, was developed to characterize the spatial structure of esca-symptomatic vines in terms of spread in any direction or within-row only. Among vineyards, a large range of spatial patterns, from random to strongly structured, associated with various prevalence rates that increased over time, were observed...
August 29, 2016: Phytopathology
Blair J Wylie, Marissa Hauptman, Alan D Woolf, Rose H Goldman
Health care providers must be equipped to provide appropriate advice to reproductive-aged patients for protection against the potentially devastating consequences of prenatal Zika virus exposure. The goal of this commentary is to summarize what is known about the safety and toxicity of N,N-diethyl-meta-toluamide (DEET) as a topical insect repellant and the pyrethroid permethrin for treatment of fabric, endorsed in the fight against Zika virus. Reviews assessing the safety and toxicity of DEET conducted by the U...
August 19, 2016: Obstetrics and Gynecology
Mattia Dall'Ara, Claudio Ratti, Salah E Bouzoubaa, David Gilmer
Viruses possessing a non-segmented genome require a specific recognition of their nucleic acid to ensure its protection in a capsid. A similar feature exists for viruses having a segmented genome, usually consisting of viral genomic segments joined together into one viral entity. While this appears as a rule for animal viruses, the majority of segmented plant viruses package their genomic segments individually. To ensure a productive infection, all viral particles and thereby all segments have to be present in the same cell...
2016: Viruses
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