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Phospholipase A2

Bhargab Kalita, Sudeepa Singh, Aparup Patra, Ashis K Mukherjee
The proteome composition of Russell's viper venom (RVV) from southern India (SI) was investigated by 1D-SDS-PAGE of venom followed by tandem mass spectrometry analysis of protein bands. A total of 66 proteins belonging to 14 snake venom protein families were identified by LC-MS/MS analysis against Viperidae (taxid 8689) protein entries from the non-redundant NCBI database. Phospholipase A2 (43.25%) and snaclec (14.57%) represented the most abundant enzymatic and non-enzymatic proteins, respectively. SI RVV was characterized as containing a higher quantity of PLA2 and a lower amount of Kunitz-type serine protease inhibitors, in comparison to RVV from other regions of the Indian subcontinent...
June 17, 2018: International Journal of Biological Macromolecules
Xu-Jie Zhou, Fu-De Zhou, Su-Xia Wang, Ming-Hui Zhao
RATIONALE: As suggested by the 2012 KDIGO guidelines, persistent elevation of serum creatinine > 3.5 mg/dl (> 309 μmol/l) (or an estimated glomerular filtration rate < 30 ml/min per 1.73 m is one of contradictions for the use of immunosuppressive therapy in membranous nephropathy. PATIENT CONCERNS: A 45-year-old man with membranous nephropathy negative for serum anti-phospholipase-A2-receptor antibody, showed no response to corticosteroids and cyclophosphamide...
June 2018: Medicine (Baltimore)
Wolfgang Stremmel, Simone Staffer, Nicole Stuhrmann, Hongying Gan-Schreier, Annika Gauss, Nina Burger, Daniel Hornuss
Background: Attack by commensal microbiota is one component of induction of inflammatory episodes in ulcerative colitis (UC). In UC, the mucus layer is intrinsically devoid of phosphatidylcholine (PC) resulting in low hydrophobicity which facilitates bacterial invasion. Colonic ectophospholipase-carrying bacterial strains are likely candidates to further thinning the PC mucus barrier and to precipitate inflammatory episodes. Objective: To evaluate the effect of phospholipase A2 (PLA2 ) inhibitors on inflammation in a genetic UC mouse model...
March 2018: Inflammatory Intestinal Diseases
Bushra Uzair, Nagina Atlas, Sidra Batool Malik, Nazia Jamil, Temitope Ojuolape Salaam, Mujaddad Ur Rehman, Barkat Ali Khan
BACKGROUND: Cancer is considered one of the most predominant causes of morbidity and mortality all over the world and colorectal cancer is the most common fatal cancers, triggering the second cancer related death. Despite progress in understanding carcinogenesis and development in chemotherapeutics, there is an essential need to search for improved treatment. More than the half a century, cytotoxic and cytostatic agents have been examined as a potential treatment of cancer, among these agents; remarkable progresses have been reported by the use of the snake venom...
June 13, 2018: Protein and Peptide Letters
Makswell Almeida Silva, Daiana Silva Lopes, Samuel Cota Teixeira, Sarah Natalie Cirilo Gimenes, Fernanda Van Petten Vasconcelos Azevedo, Lorena Polloni, Bruna Cristina Borges, Marcelo Santos da Silva, Marcelo José Barbosa, Robson José de Oliveira Júnior, Maria Carolina Elias, Claudio Vieira da Silva, Kelly Aparecida Geraldo Yoneyama, Veridiana de Melo Rodrigues, Renata Santos Rodrigues
Herein we evaluated the genotoxic effects of BnSP-6, a Lys-49 phospholipase A2 (PLA2 ) from Bothrops pauloensis, on breast cancer cells. BnSP-6 was able to induce a higher cytotoxic and genotoxic activity in MDA-MB-231 cells, when compared to MCF10A (a non-tumorigenic breast cell line), suggesting that this protein presented a possible preference for cancer cells. BnSP-6 inhibited MDA-MB-231 proliferation at 24, 48 and 72 h. In addition, BnSP-6 induced significant increase in the percentage of TUNEL-positive cells, a marker of DNA damage...
June 16, 2018: International Journal of Biological Macromolecules
Maria C Fernandez, Cristian O'Flaherty
STUDY QUESTION: Are all components of the peroxiredoxins (PRDXs) system important to control the levels of reactive oxygen species (ROS) to maintain viability and DNA integrity in spermatozoa? SUMMARY ANSWER: PRDX6 is the primary player of the PRDXs system for maintaining viability and DNA integrity in human spermatozoa. WHAT IS KNOWN ALREADY: Mammalian spermatozoa are sensitive to high levels of ROS and PRDXs are antioxidant enzymes proven to control the levels of ROS generated during sperm capacitation to avoid oxidative damage in the spermatozoon...
June 15, 2018: Human Reproduction
Vahideh Ghorani, Narges Marefati, Farzaneh Shakeri, Ramin Rezaee, Marzie Boskabady, Mohammad Hossein Boskabady
Antioxidant, antimicrobial, anti-hyperglycaemic, anti-diabetic and anti-inflammatory effects of Allium cepa (A. cepa) have been previously shown. In this study, the effects of A. cepa aqueous-alcoholic extract on tracheal responsiveness, lung inflammatory cells and phospholipase A2 (PLA2) level in bronchoalveolar fluid (BALF) of asthmatic rats were examined. Wistar rats were randomly divided into control group (C), asthmatic group (A), asthmatic group (A) treated with A. cepa extract (AC, 0.175, 0.35, and 0...
June 2018: Iranian Journal of Allergy, Asthma, and Immunology
Adrian Florea, Andrei Patrick Varga, Horea Vladi Matei
We tested the ability of bee venom (BV), melittin (Mlt), and phospholipase A2 (PLA) - used in 5 concentrations each (5, 10, 15, 20 and 40 μg/100 μl) - to promote ultrastructural changes and reorganization of cristae in vitro in mitochondria isolated from rat adrenal cortex after a protocol optimized by us. Thus, apart from two control grups (CI and CS), in which the mitochondria were suspended into saline buffer and isolation medium respectively, 15 more groups of mitochondria were constituted, corresponding to the five different doses of the three substance tested (BV5 to M40; M5 to M40 and P5 to P40)...
June 9, 2018: Micron: the International Research and Review Journal for Microscopy
Jacqueline S Stevens, Mary C Gray, Christophe Morisseau, Alison K Criss
Background: Infection with Neisseria gonorrhoeae (Ngo) is characterized by robust neutrophil influx that is insufficient to clear the bacteria. Sustained neutrophilic inflammation contributes to serious clinical sequelae that particularly affect women, including pelvic inflammatory disease and infertility. Methods: We established a three-component system using Ngo, End1 polarized human endocervical cells, and primary human neutrophils to investigate neutrophil transepithelial migration following infection...
June 13, 2018: Journal of Infectious Diseases
Eva Jarc, Thomas O Eichmann, Robert Zimmermann, Toni Petan
The data presented here is related to the research article entitled "Lipid droplets induced by secreted phospholipase A2 and unsaturated fatty acids protect breast cancer cells from nutrient and lipotoxic stress" by E. Jarc et al., Biochim. Biophys. Acta 1863 (2018) 247-265. Elevated uptake of unsaturated fatty acids and lipid droplet accumulation are characteristic of aggressive cancer cells and have been associated with the cellular stress response. The present study provides lipidomic data on the triacylglycerol (TAG) and phosphatidylcholine (PC) composition of MDA-MB-231 breast cancer cells exposed to docosahexaenoic acid (DHA; 22:6, ω-3)...
June 2018: Data in Brief
Brahim Gargouri, Johanna Carstensen, Harsharan S Bhatia, Michael Huell, Gunnar P H Dietz, Bernd L Fiebich
BACKGROUND: Neuroinflammation is a key factor of Alzheimer's disease (AD) and other neurodegenerative conditions. Microglia are the resident mononuclear immune cells of the central nervous system (CNS). They play an essential role in the maintenance of homeostasis and responses to neuroinflammation. Ginkgo biloba extract EGb 761 is one of the most commonly used natural medicines owing to its established efficacy and remarkable biological activities especially in respect to CNS diseases...
May 15, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Honglu Chao, Yinlong Liu, Chao Lin, Xiupeng Xu, Zheng Li, Zhongyuan Bao, Liang Fan, Chao Tao, Lin Zhao, Yan Liu, Xiaoming Wang, Yongping You, Ning Liu, Jing Ji
Phospholipase A2 is a known aggravator of inflammation and deteriorates neurological outcomes after traumatic brain injury (TBI), however the exact inflammatory mechanisms remain unknown. This study investigated the role of bradykinin and its receptor, which are known initial mediators within inflammation activation, as well as the mechanisms of the cytosolic phospholipase A2 (cPLA2 )-related inflammatory responses after TBI. We found that cPLA2 and bradykinin B2 receptor were upregulated after a TBI. Rats treated with the bradykinin B2 receptor inhibitor LF 16-0687 exhibited significantly less cPLA2 expression and related inflammatory responses in the brain cortex after sustaining a controlled cortical impact (CCI) injury...
June 9, 2018: Biochimica et Biophysica Acta
Cai-Yun Ma, Zhen-Ye Xu, Shao-Ping Wang, Hong-Yu Peng, Fang Liu, Jing-Hua Liu, Feng-Xue Ren
Background: Acute coronary syndrome (ACS) is closely related to unstable plaques and secondary thrombosis. The inflammatory cells in plaques and their inflammatory products may be the cause for plaque instability and ruptures. The study aimed to disclose the changes of inflammatory factors including serum intracellular adhesion molecule-1 (ICAM-1), chitinase-3-like protein 1 (YKL-40), and lipoprotein-associated phospholipase A2 (Lp-PLA2) in patients with ACS and its clinical significance...
June 20, 2018: Chinese Medical Journal
Limin Huang, Dazhun Zhang, Yan Wang, Qiao Zhang, Wei Liang, Yongding Zhang, Zuying Xiong
To develop and evaluate an ELISA kit for Anti-PLA2R IgG. Recombinant M-type phospholipase A2 receptor (PLA2R) protein expressed in HEK293 was taken as coating antigen, HRP-labeled rabbit anti-human IgG was taken as a tracer, to test the anti-PLA2R IgG on the basis of the principle of ELISA. The detection linear range, accuracy, linear correlation, repeatability, and stability were evaluated. In addition, we made a comparison with enrolled anti-PLA2R IgG kit. The detection linear range of the kit is no more than 2 RU/mL...
May 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Juan Bai, Ying Zhu, Ying Dong
Obesity is known to induce pathological changes in the gut and diets rich in complex carbohydrates that resist digestion in the small bowel can alter large bowel ecology. The purposes of this study were to identify the effects of bitter melon powder (BMP) on the global gene expression pattern in the colon mucosa of obese rats. Obese rats were fed a high-fat diet and treated without or with BMP for 8 weeks. Genome-wide expression profiles of the colon mucosa were determined by RNA sequencing (RNA-Seq) analysis at the end of experiment...
June 2018: Genes & Genomics
Vittorio Caprio, Lina Badimon, Mario Di Napoli, Wen-Hui Fang, Glenn R Ferris, Baoqiang Guo, Rocco S Iemma, Donghui Liu, Yasmin Zeinolabediny, Mark Slevin
Circulating C-reactive protein (CRP) is a key acute-phase protein and one of the main clinical biomarkers for inflammation and infection. CRP is an important upstream mediator of inflammation and is associated with the onset of a number of important disease states including cardiovascular disease and neurodegenerative disorders such as Alzheimer's disease. This pentraxin exerts pro-inflammatory properties via dissociation of the pentamer (pCRP) to a monomeric form (mCRP). This dissociation is induced by binding of pCRP to cell surface phosphocholine residues exposed by the action of phospholipase A2 (PLA2 )...
2018: Frontiers in Immunology
Julio Vindas, Yarina Carrera, Bruno Lomonte, José María Gutiérrez, Juan J Calvete, Libia Sanz, Julián Fernández
The first toxin isolated from the venomous pit viper Porthidium ophryomegas is a basic pentameric phospholipase A2 (PophPLA2 ). Elucidation of its amino acid sequence showed that it belongs to the group IIA of secreted PLA2 s, with the presence of all 14 conserved cysteine positions. The toxin displayed catalytic activity, in agreement with the presence of Asp49 in its sequence of 121 residues. SDS-PAGE analysis revealed that this toxin is pentameric in non-reducing conditions, a structural organization that has not been described for any viperid PLA2 ...
June 7, 2018: International Journal of Biological Macromolecules
Abir Ben Bacha, Mona Awad Alonazi, Mohamed Solman Elshikh, Aida Karray
A novel non-toxic phospholipase A2 was purified to homogeneity in a single chromatography step from the venom of Walterinnesia aegyptia, a monotypic elapid snake caught in Saudi Arabia, and its antimicrobial and hemolytic properties were evaluated as well. This enzyme, namely WaPLA2 , is a homodimer with an estimated molecular mass of 30 kDa, and its NH2 -terminal sequence exhibits a significant degree of similarity with PLA2 group-I. At optimal pH (8.5) and temperature (45 °C), the purified PLA2 exhibited a specific activity of 2100 U/mg, and it requires bile salts and Ca2+ for its activity...
June 6, 2018: International Journal of Biological Macromolecules
Lei Liu, Baochao Chang, Xueping Wu, Yaling Guo, Yan Pan, Lijuan Yang
Objectives: The aims of this study were to detect the expression of M phospholipase A2 receptor (PLA2R) in the kidney tissue of patients with idiopathic membranous nephropathy (IMN), secondary membranous nephropathy (SMN), and the nonmembranous nephropathy (non-MN), to evaluate the value of PLA2R in the kidney tissue and serum anti-PLA2R antibody in the diagnosis of membranous nephropathy (MN), and to explore the relationship between PLA2R of the kidney tissue or serum anti-PLA2R antibody and clinical features of MN...
2018: Vascular Health and Risk Management
Wenli Wu, Jin Shang, Chenyang Tao, Shuai Wang, Xiaoru Hu, Shiyi Zhang, Zhanzheng Zhao
BACKGROUND: The presence of antibodies against phospholipase A2 receptor (PLA2R-Abs) in serum at diagnosis is reported to be related to the rate of spontaneous remission in patients with idiopathic membranous nephropathy (IMN); however, there is still lack of enough samples to illustrate this problem. Here, we conducted a comprehensive meta-analysis to investigate the prognostic value of PLA2R-Abs on spontaneous remission for IMN patients in the absence of immunosuppressive therapy. METHODS: A systematic search of the PubMed, EMBASE, and Cochrane Central databases was performed for relevant original articles published until October 2017...
June 2018: Medicine (Baltimore)
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