keyword
MENU ▼
Read by QxMD icon Read
search

Phospholipase A2

keyword
https://www.readbyqxmd.com/read/28645780/salvia-miltiorrhiza-bunge-danshen-extract-attenuates-permanent-cerebral-ischemia-through-inhibiting-platelet-activation-in-rats
#1
Yu-Xiang Fei, Si-Qi Wang, Li-Jian Yang, Yan-Ying Qiu, Yi-Ze Li, Wen-Yuan Liu, Tao Xi, Wei-Rong Fang, Yun-Man Li
ETHNOPHARMACOLOGICAL RELEVANCE: Danshen is a crude herbal drug isolated from dried roots of Salvia miltiorrhiza Bunge. This plant is widely used in oriental medicine for the treatment of cardiovascular and cerebrovascular diseases. The supercritical CO2 extract from Danshen (SCED) (57.85%, 5.67% and 4.55% for tanshinone IIA, tanshinone I and cryptotanshinone respectively) was studied in this article, whose potential molecular mechanism remains unclear, especially in anti-thrombosis. AIM OF THE STUDY: The present study was designed to observe the protective effect of SCED on ischemic stroke in rats and to explore the underlying anti-thrombosis mechanism...
June 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28644968/serum-lipoprotein-associated-phospholipase-a2-predicts-the-formation-of-carotid-artery-plaque-and-its-vulnerability-in-anterior-circulation-cerebral-infarction
#2
Yuping Yang, Tao Xue, Juehua Zhu, Jiayi Xu, Xiaowei Hu, Penghao Wang, Tao Kong, Yan Yan, Lihui Yang, Shouru Xue
OBJECTIVE: Circulation inflammation markers such as high-sensitive C-reactive protein (hsCRP) and lipoprotein-associated phospholipase A2 (Lp-PLA2) are considered as predictors of cerebral and cardiac vascular diseases. However, the role of hsCRP and Lp-PLA2 in the anterior circulation cerebral infarction (ACI) is to be elaborated. PATIENTS AND METHODS: We included 100 patients with acute anterior circulation cerebral infarction (AaCI group) and 50 non-infarction subjects (control group)...
June 12, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/28639618/htlv-1-induced-leukotriene-b4-secretion-by-t-cells-promotes-t-cell-recruitment-and-virus-propagation
#3
Florent Percher, Céline Curis, Eléonore Pérès, Maria Artesi, Nicolas Rosewick, Patricia Jeannin, Antoine Gessain, Olivier Gout, Renaud Mahieux, Pierre-Emmanuel Ceccaldi, Anne Van den Broeke, Madeleine Duc Dodon, Philippe V Afonso
The human T-lymphotropic virus type 1 (HTLV-1) is efficiently transmitted through cellular contacts. While the molecular mechanisms of viral cell-to-cell propagation have been extensively studied in vitro, those facilitating the encounter between infected and target cells remain unknown. In this study, we demonstrate that HTLV-1-infected CD4 T cells secrete a potent chemoattractant, leukotriene B4 (LTB4). LTB4 secretion is dependent on Tax-induced transactivation of the pla2g4c gene, which encodes the cytosolic phospholipase A2 gamma...
June 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28637652/gm-csf-and-m-csf-primed-m%C3%AF-s-present-similar-resolving-but-distinct-inflammatory-lipid-mediator-signatures
#4
Ana Lukic, Pia Larssen, Alexander Fauland, Bengt Samuelsson, Craig E Wheelock, Susanne Gabrielsson, Olof Radmark
M1 and M2 activated Mϕs have different roles in inflammation. Because pathogens may first encounter resting cells, we investigated lipid mediator profiles prior to full activation. Human monocytes were differentiated with granulocyte Mϕ colony-stimulating factor (GM-CSF) or Mϕ colony-stimulating factor (M-CSF), which are known to prime toward M1 or M2 phenotypes, respectively. Lipid mediators released during resting conditions and produced in response to bacterial stimuli (LPS/N-formylmethionyl-leucyl-phenylalanine or peptidoglycan) were quantified by liquid chromatography-mass spectrometry...
June 21, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28633930/phospholipase-a2-in-the-venom-of-three-cottonmouth-snakes
#5
Ying Jia, Boris Ermolinsky, Aryana Garza, Daniele Provenzano
Two phospholipase A2s (PLA2s), Asp49 (D49) and Lys49 (K49), were purified by one-step reverse-phase high performance liquid chromatography (RP-HPLC) from the venom of each of the three subspecies of cottonmouth snake, Western cottonmouth (Agkistrodon piscivorus leucostoma; Apl), Eastern cottonmouth (Agkistrodon piscivorus piscivorus; App) and Florida cottonmouth (Agkistrodon piscivorus conanti; Apc). Venom protein profiles and PLA2s elution pattern of the three cottonmouth snakes were remarkably similar displaying four similar sharp and two wide peaks; in all cases K49 PLA2 eluted first followed by D49 PLA2...
June 17, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28627471/phospholipid-drug-conjugates-as-a-novel-oral-drug-targeting-approach-for-the-treatment-of-inflammatory-bowel-disease
#6
Arik Dahan, Milica Markovic, Svetlana Epstein, Noa Cohen, Ellen M Zimmermann, Aaron Aponick, Shimon Ben-Shabat
The enzyme phospholipase A2 (PLA2) is overexpressed in the inflamed intestine in inflammatory bowel disease (IBD) patients, and in this work we aimed to exploit PLA2 as a prodrug-activating enzyme for a novel PL-drug conjugate, thereby liberating the free drug specifically in the targeted diseased tissue(s). The proposed prodrug contains a drug moiety covalently bound through a linker to the sn-2 position of a phospholipid (PL). The NSAID diclofenac was used as model molecule, and four different linker lengths (2, 4, 6 and 8 -CH2 units) were studied...
June 13, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28625888/combined-venom-profiling-and-cytotoxicity-screening-of-the-radde-s-mountain-viper-montivipera-raddei-and-mount-bulgar-viper-montivipera-bulgardaghica-with-potent-cytotoxicity-against-human-a549-lung-carcinoma-cells
#7
Ayse Nalbantsoy, Benjamin-Florian Hempel, Daniel Petras, Paul Heiss, Bayram Göçmen, Nasit Iğci, Mehmet Zülfü Yildiz, Roderich D Süssmuth
Here we report the first characterization of the endemic Mount Bulgar Viper (Montivipera bulgardaghica) and Radde's mountain viper (Montivipera raddei) venom by a combined approach using intact mass profiling and bottom-up proteomics. The cytotoxicity screening of crude venom as well as isolated serine proteases revealed a high activity against A549 human lung carcinoma cells. By means of intact mass profiling of native and reduced venom we observed basic and acidic phospholipases type A2. Moreover, the analysis revealed snake venom metalloproteases, cysteine-rich secretory proteins, disintegrins, snake venom serine proteases, C-type lectins, a vascular endothelial growth factor and an L-amino acid oxidase...
June 16, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28625237/-study-on-mechanisms-and-myocardial-protective-effect-of-qishen-yiqi-dropping-pills-on-rats-with-myocardial-infarction
#8
Quan Yang, Yunshan Cao
OBJECTIVE: To approach the mechanisms and myocardial protective effect of Qishen Yiqi dropping pills on rats with myocardial infarction. METHODS: Sixty clean healthy male Sprague-Dawley (SD) rats were randomly divided into sham operation group, model group and observation group (each n = 20). The rat model of acute myocardial infarction (AMI) was established by ligation of left anterior descent (LAD) branch of coronary artery. After modeling, the rats in observation group were given 0...
June 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28624308/efficient-heterologous-expression-functional-characterization-and-molecular-modeling-of-annular-seabream-digestive-phospholipase-a2
#9
Nabil Smichi, Houcemeddine Othman, Neila Achouri, Alexandre Noiriel, Soumaya Triki, Vincent Arondel, Najet Srairi-Abid, Abdelkarim Abousalham, Youssef Gargouri, Nabil Miled, Ahmed Fendri
Here we report the cDNA cloning of a phospholipase A2 (PLA2) from five Sparidae species. The deduced amino acid sequences show high similarity with pancreatic PLA2. In addition, a phylogenetic tree derived from alignment of various available sequences revealed that Sparidae PLA2 are closer to avian PLA2 group IB than to mammals' ones. In order to understand the structure-function relationships of these enzymes, we report here the recombinant expression in E.coli, the refolding and characterization of His-tagged annular seabream PLA2 (AsPLA2)...
June 14, 2017: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/28623974/increased-placental-phospholipase-a2-gene-expression-and-free-f2-isoprostane-levels-in-response-to-oxidative-stress-in-preeclampsia
#10
M Brien, J Larose, K Greffard, P Julien, J F Bilodeau
Vasoactive eicosanoids such as thromboxane (TX) A2 and F2-isoprostanes (F2-isoPs) were shown to be increased in the preeclamptic placenta. Only one of the 64 possible isomers of F2-isoPs derived from the oxidation of arachidonic acid was investigated in the placenta so far. F2-isoPs are released from membrane phospholipids by phospholipases A2 (PLA2) and were shown to act on the TXA2 receptor (TBXA2R). However, the PLA2 deregulated in preeclampsia (PE) remains to be determined. In this study, we analyzed the concentrations of six isomers of F2-isoPs; 8-iso-PGF2α, 8-iso-15(R)-PGF2α, 15(R)-PGF2α, iPF2α-IV, iPF2α-VI, 5-iPF2α-VI and the concentrations of the stable metabolites of TXA2, TXB2, by high performance liquid chromatography coupled with tandem mass spectrometry in placentas of PE (n = 17) and normotensive (n = 15) pregnancies according to the biopsy site: peri-insertion or periphery...
July 2017: Placenta
https://www.readbyqxmd.com/read/28618256/cigarette-smoke-regulates-calcium-independent-phospholipase-a2-metabolic-pathways-in-breast-cancer
#11
Shannon Kispert, Theresa Schwartz, Jane McHowat
Phospholipase A2 (PLA2)-dependent pathways are important in the regulation of cell proliferation, differentiation, motility, and immune responses, and can be dysregulated during tumor development and progression. We show herein, for the first time, that cigarette smoking leads to an increase in platelet-activating factor (PAF) content and PAF receptor expression in human breast cancer cells and tissue. PAF production could be abrogated in triple-negative breast cancer cells by inhibition of calcium-independent PLA2 (iPLA2)...
June 12, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28616616/lipoprotein-associated-phospholipase-a2-activity-and-its-diagnostic-potential-in-patients-with-acute-coronary-syndrome-and-acute-ischemic-stroke
#12
Sedat Kocak, Birsen Ertekin, Abdullah Sadik Girisgin, Zerrin Defne Dundar, Mehmet Ergin, Idris Mehmetoglu, Said Bodur, Basar Cander
BACKGROUND: The study examined the Lp-PLA2 activity at the patients presented to the emergency department with acute coronary syndrome (ACS) or acute ischemic stroke (AIS), as well as its diagnostic value. METHODS: The prospective study included consecutive male and female patients aged >18 years that presented to the our emergency department with ACS or AIS between November 2009 and January 2010. Blood samples were obtained immediately following diagnosis in the ACS and AIS groups...
June 2017: Turkish Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28614271/serum-anti-pla2r-antibody-and-glomerular-pla2r-deposition-in-chinese-patients-with-membranous-nephropathy-a-cross-sectional-study
#13
Lu Pang, Ai-Min Zhang, Hai-Xia Li, Jia-Lin Du, Li-Li Jiao, Nan Duan, Yi Liu, Dan Yu
M-type phospholipase A2 receptor (PLA2R) is the major target antigen in primary membranous nephropathy (PMN). Previous studies have evaluated the diagnostic value of serum anti-PLA2R antibody. However, the correlation of serum anti-PLA2R antibody and glomerular PLA2R deposition, and their association with clinical characteristics need to be further evaluated.A total of 136 patients were involved as inception group because serum anti-PLA2R antibody and glomerular PLA2R antigen were simultaneously measured. We examined serum anti-PLA2R antibody by ELISA and glomerular PLA2R deposition by immunofluorescence assay...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28613145/two-novel-dromedary-camel-bocaparvoviruses-from-dromedaries-in-the-middle-east-with-unique-genomic-features
#14
Patrick C Y Woo, Susanna K P Lau, Hoi-Wah Tsoi, Nissy Georgy Patteril, Hazel C Yeung, Sunitha Joseph, Emily Y M Wong, Rubeena Muhammed, Franklin W N Chow, Ulrich Wernery, Kwok-Yung Yuen
The recent emergence of Middle East respiratory syndrome (MERS) coronavirus and its discovery from dromedary camels has boosted interest in the search for novel viruses in dromedaries. While bocaparvoviruses are known to infect various animals, it was not known that they exist in dromedaries. In this study, we describe the discovery of two novel dromedary camel bocaparvoviruses (DBoVs), DBoV1 and DBoV2, from dromedary faecal samples in Dubai. Among 667 adult dromedaries and 72 dromedary calves, 13.9 % of adult dromedaries and 33...
June 14, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28609844/an-overview-on-immunoregulatory-and-anti-inflammatory-properties-of-chrysin-and-flavonoids-substances
#15
REVIEW
Majid Zeinali, Seyed Abdolrahim Rezaee, Hossein Hosseinzadeh
Inflammation and the pro-inflammatory cytokines are associated with numerous chronic diseases. Studies suggest that flavonoids, plant polyphenolic compound derivatives from natural origin, have a wide range of putative biological activities. Similar to other flavonoids, chrysin (CH) by its anti-oxidative and anti-inflammatory effects is a potential prophylactic agent in immunopathological and physicochemical injuries. This is an overview on putative immunomodulatory activities of flavonoids and beneficial health effects of these substances particularly, CH in the immune system...
June 9, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28608598/purification-and-partial-characterization-of-an-anticoagulant-pla2-from-the-venom-of-indian-daboia-russelii-that-induces-inflammation-through-upregulation-of-proinflammatory-mediators
#16
Archana Deka, Maitreyee Sharma, Manoj Sharma, Rupak Mukhopadhyay, Robin Doley
The present study describes the purification and partial characterization of a basic anticoagulant PLA2 enzyme named as Rv(i) PLA2 from the venom of Indian Daboia russelii. The molecular mass of the protein was found to be 13,659.65 Da, and peptide mass fingerprinting revealed that it belongs to group II PLA2 family. The peptide sequence showed similarity to uncharacterized basic PLA2 enzyme having an accession no. of P86368 reported from Sri Lankan D. russelii. Rv(i) PLA2 exhibited strong phospholipase A2 and anticoagulant activity...
June 13, 2017: Journal of Biochemical and Molecular Toxicology
https://www.readbyqxmd.com/read/28608449/resveratrol-suppresses-lipoprotein-associated-phospholipase-a2-expression-by-reducing-oxidative-stress-in-macrophages-and-animal-models
#17
Shengnan Sun, Mingjun Zhang, Qiangbing Yang, Ziying Shen, Jiahuan Chen, Biao Yu, He Wang, Jiali Qu, Daxin Pang, Wenzhi Ren, Hongsheng Ouyang, Xiaochun Tang
SCOPE: Resveratrol is a naturally occurring polyphenolic compound with known cardioprotective, anti-inflammatory and antioxidant properties. Lipoprotein-associated phospholipase A2 (Lp-PLA2 ) is associated with the risk of cardiovascular disease. Here, we investigated the effects of resveratrol on Lp-PLA2 expression in vitro and in vivo and explored the underlying mechanisms. METHODS AND RESULTS: Human monocytic cells (THP-1) were induced to differentiate into macrophages for an in vitro experimental model...
June 13, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28603593/markers-of-inflammation-and-cardiovascular-disease-in-recently-diagnosed-celiac-disease-patients
#18
Walter F Tetzlaff, Tomás Meroño, Martin Menafra, Maximiliano Martin, Eliana Botta, Maria D Matoso, Patricia Sorroche, Juan A De Paula, Laura E Boero, Fernando Brites
AIM: To evaluate novel risk factors and biomarkers of cardiovascular disease in celiac disease (CD) patients compared with healthy controls. METHODS: Twenty adult patients with recent diagnosis of CD and 20 sex, age and body mass index-matched healthy controls were recruited during a period of 12 mo. Indicators of carbohydrate metabolism, hematological parameters and high sensitive C reactive protein were determined. Moreover, lipoprotein metabolism was also explored through evaluation of the lipid profile and the activity of cholesteryl ester transfer protein and lipoprotein associated phospholipase A2, which is also considered a specific marker of vascular inflammation...
May 26, 2017: World Journal of Cardiology
https://www.readbyqxmd.com/read/28602829/comparison-of-proteomic-profiles-of-the-venoms-of-two-of-the-big-four-snakes-of-india-the-indian-cobra-naja-naja-and-the-common-krait-bungarus-caeruleus-and-analyses-of-their-toxins
#19
Manisha Choudhury, Ryan J R McCleary, Manish Kesherwani, R Manjunatha Kini, Devadasan Velmurugan
Snake venoms are mixtures of biologically-active proteins and peptides, and several studies have described the characteristics of some of these toxins. However, complete proteomic profiling of the venoms of many snake species has not yet been done. The Indian cobra (Naja naja) and common krait (Bungarus caeruleus) are elapid snake species that are among the 'Big Four' responsible for the majority of human snake envenomation cases in India. As understanding the composition and complexity of venoms is necessary for successful treatment of envenomation in humans, we utilized three different proteomic profiling approaches to characterize these venoms: i) one-dimensional SDS-PAGE coupled with in-gel tryptic digestion and electrospray tandem mass spectrometry (ESI-LC-MS/MS) of individual protein bands; ii) in-solution tryptic digestion of crude venoms coupled with ESI-LC-MS/MS; and iii) separation by gel-filtration chromatography coupled with tryptic digestion and ESI-LC-MS/MS of separated fractions...
June 8, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28602828/individual-variability-of-venom-from-the-european-adder-vipera-berus-berus-from-one-locality-in-eastern-hungary
#20
Tamás Malina, László Krecsák, Alexander Westerström, Gábor Szemán-Nagy, Gyöngyi Gyémánt, Márta M-Hamvas, Edward G Rowan, Alan L Harvey, David A Warrell, Balázs Pál, Zoltán Rusznák, Gábor Vasas
We have revealed intra-population variability among venom samples from several individual European adders (Vipera berus berus) within a defined population in Eastern Hungary. Individual differences in venom pattern were noticed, both gender-specific and age-related, by one-dimensional electrophoresis. Gelatin zymography demonstrated that these individual venoms have different degradation profiles indicating varying protease activity in the specimens from adders of different ages and genders. Some specimens shared a conserved region of substrate degradation, while others had lower or extremely low protease activity...
June 9, 2017: Toxicon: Official Journal of the International Society on Toxinology
keyword
keyword
14557
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"