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Phospholipase A2

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https://www.readbyqxmd.com/read/29442225/feeder-cell-independent-culture-of-the-pig-embryonic-stem-cell-derived-exocrine-pancreatic-cell-line-picm-31
#1
Neil C Talbot, Amy E Shannon, Caitlin E Phillips, Wesley M Garrett
The adaptation to feeder-independent growth of a pig embryonic stem cell-derived pancreatic cell line is described. The parental PICM-31 cell line, previously characterized as an exocrine pancreas cell line, was colony-cloned two times in succession resulting in the derivative cell line, PICM-31A1. PICM-31A1 cells were adapted to growth on a polymerized collagen matrix using feeder cell-conditioned medium and were designated PICM-31FF. Like the parental cells, the PICM-31FF cells were small and grew relatively slowly in closely knit colonies that eventually coalesced into a continuous monolayer...
February 13, 2018: In Vitro Cellular & Developmental Biology. Animal
https://www.readbyqxmd.com/read/29442044/microrna-3188-targets-ets-domain-protein-4-and-participates-in-rhoa-rock-pathway-to-regulate-the-development-of-atherosclerosis
#2
Na Li, Jinghong Chen, Jingru Zhao, Tianjun Wang
We aimed to elucidate the roles and regulatory mechanism of miR-3188 in oxidized low-density lipoprotein (ox-LDL)-induced cell injury in THP-1 derived macrophages, thus providing a new insight for the treatment of atherosclerosis (AS). A total of 85 AS patients and 45 healthy controls were enrolled. The levels of miR-3188 and lipoprotein-associated phospholipase A2 (Lp-PLA2) in AS patients and healthy controls were detected. Then ox-LDL was used to treat human THP-1 derived macrophages. The effects of overexpression and suppression of miR-3188 on regulating ox-LDL-induced cell injury in THP-1 derived macrophages were investigated...
November 1, 2017: Die Pharmazie
https://www.readbyqxmd.com/read/29441742/the-effect-of-mycophenolate-mofetil-versus-cyclosporine-as-combination-therapy-with-low-dose-corticosteroids-in-high-risk-patients-with-idiopathic-membranous-nephropathy-a-multicenter-randomized-trial
#3
Ji Young Choi, Dong Ki Kim, Yang Wook Kim, Tae Hyun Yoo, Jung Pyo Lee, Hyun Chul Chung, Kyu Hyang Cho, Won Suk An, Duk Hyun Lee, Hee Yeon Jung, Jang Hee Cho, Chan Duck Kim, Yong Lim Kim, Sun Hee Park
BACKGROUND: Appropriate immunosuppressive therapy for patients with idiopathic membranous nephropathy (MN) remains controversial. The effect of mycophenolate mofetil (MMF) versus cyclosporine (CsA) combined with low-dose corticosteroids was evaluated in patients with idiopathic MN in a multi-center randomized trial (www.ClinicalTrials.gov NCT01282073). METHODS: A total of 39 biopsy-proven idiopathic MN patients with severe proteinuria were randomly assigned to receive MMF combined with low-dose corticosteroids (MMF group) versus CsA combined with low-dose corticosteroids (CsA group), respectively, and followed up for 48 weeks...
February 26, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29433083/inhibition-of-phospholipases-influences-the-metabolism-of-wound-induced-benzylisoquinoline-alkaloids-in-papaver-somniferum-l
#4
Veronika Jablonická, Jörg Ziegler, Zuzana Vatehová, Desana Lišková, Ingo Heilmann, Marek Obložinský, Mareike Heilmann
Benzylisoquinoline alkaloids (BIAs) are important secondary plant metabolites and include medicinally relevant drugs, such as morphine or codeine. As the de novo synthesis of BIA backbones is (still) unfeasible, to date the opium poppy plant Papaver somniferum L. represents the main source of BIAs. The formation of BIAs is induced in poppy plants by stress conditions, such as wounding or salt treatment; however, the details about regulatory processes controlling BIA formation in opium poppy are not well studied...
January 31, 2018: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29428619/effect-of-dietary-n-3-polyunsaturated-fatty-acid-rich-fish-oil-on-the-endometrial-prostaglandin-production-in-the-doe-capra-hircus
#5
Ravjibhai K Chaudhari, Ajit Singh Mahla, Amit Kumar Singh, Sanjay Kumar Singh, Abhijit M Pawde, G V P P S Ravi Kumar, GyanendraSingh, Mihir Sarkar, Harendra Kumar, Krishnaswamy Narayanan
Recently, we showed that dietary supplementation of n-3 PUFA rich fish oil (FO) decreased the metabolites of serum prostaglandin (PG) F 2α and E 2 during the window of pregnancy recognition in the doe. In this study, we investigated its effect on the changes on endometrial PG production in vitro. Cycling does (n = 12) of Rohilkhand region were divided into two equal groups and fed a concentrate diet supplemented with either FO containing 26% n-3 PUFA (TRT; n = 6) or palm oil (CON; n = 6) @ 0.6 mL/kg body weight for 57 days...
February 8, 2018: Prostaglandins & Other Lipid Mediators
https://www.readbyqxmd.com/read/29427679/met117-oxidation-leads-to-enhanced-flexibility-of-cardiovascular-biomarker-lipoprotein-associated-phospholipase-a2-and-reduced-substrate-binding-affinity-with-platelet-activating-factor
#6
Arun Bahadur Gurung, Atanu Bhattacharjee
Human Lipoprotein-associated phospholipase A2 (Lp-PLA2) is an important biomarker for cardiovascular diseases and a therapeutically important drug target against Atherosclerosis. It has the ability to hydrolyze various oxidized low density lipoproteins (LDL) and generates potent pro-inflammatory signaling molecules. Both physiological and non-physiological oxidants have been reported to inhibit Lp-PLA2 activity. The mechanism of the enzyme inhibition due to oxidation of surface exposed Met117 at the structural level is not clearly understood...
February 7, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29427237/differential-expression-of-lp-pla2-in-obesity-and-type-2-diabetes-and-the-influence-of-lipids
#7
Laura Jackisch, Warunee Kumsaiyai, Jonathan D Moore, Nasser Al-Daghri, Ioannis Kyrou, Thomas M Barber, Harpal Randeva, Sudhesh Kumar, Gyanendra Tripathi, Philip G McTernan
AIMS/HYPOTHESIS: Lipoprotein-associated phospholipase A2 (Lp-PLA2) is a circulatory macrophage-derived factor that increases with obesity and leads to a higher risk of cardiovascular disease (CVD). Despite this, its role in adipose tissue and the adipocyte is unknown. Therefore, the aims of this study were to clarify the expression of Lp-PLA2 in relation to different adipose tissue depots and type 2 diabetes, and ascertain whether markers of obesity and type 2 diabetes correlate with circulating Lp-PLA2...
February 9, 2018: Diabetologia
https://www.readbyqxmd.com/read/29424477/association-of-lipoprotein-associated-phospholipase-a2-mass-with-asymptomatic-cerebral-artery-stenosis
#8
Youxin Wang, Bin Zhou, Pingan Zhou, Yan Yao, Qinghua Cui, Yingping Liu, Jichun Yang, Shouling Wu, Xingquan Zhao, Yong Zhou
Cerebral artery stenosis (CAS) is the most important causes of ischaemic stroke. Lipoprotein-associated phospholipase A2 (Lp-PLA2) plays 2 diverse roles in atherosclerosis (pro-inflammatory and anti-inflammatory), and the association between Lp-PLA2 mass and cardiovascular or cerebrovascular events is inconsistent among previous studies. A cross-sectional study including 2012 North Chinese adults aged ≥40 years was performed in 2010-2011 to investigate whether Lp-PLA2 mass is associated with asymptomatic cerebral artery stenosis (ACAS)...
February 9, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29420993/glucagon-like-peptide-2-improves-intestinal-immune-function-and-diminishes-bacterial-translocation-in-a-mouse-model-of-parenteral-nutrition
#9
Qiucheng Lei, Jingcheng Bi, Huanwei Chen, Feng Tian, Xuejin Gao, Ning Li, Xinying Wang
Parenteral nutrition (PN) is associated with increased infectious risks due to impaired intestinal immunity. Although glucagon-like peptide-2 (GLP-2) enhances the gut barrier function, it is uncertain whether it improves mucosal immunologic barrier function. We hypothesized that injecting the PN mouse model with GLP-2 improved innate and acquired immunity, and prevented bacterial translocation. Forty-eight hours after venous cannulation, male Institute of Cancer Research mice were randomly divided into 3 groups based on their diet: chow with saline (n = 10), PN (n = 9), or PN + GLP-2 (30 μg bid per mouse, n = 10) provided for 5 days...
January 2018: Nutrition Research
https://www.readbyqxmd.com/read/29411531/aspirin-and-clopidogrel-high-on-treatment-platelet-reactivity-and-genetic-predictors-in-peripheral-arterial-disease
#10
Khung-Keong Yeo, Ehrin J Armstrong, Javier E López, Debbie C Chen, Gregory G Westin, Chin-Shang Li, David Anderson, Amy Hua, Anil Singapuri, Ezra A Amsterdam, Nipavan Chiamvimonvat, John R Laird
OBJECTIVES: Our aims were to examine the prevalence and genetic predictors of aspirin and clopidogrel high on-treatment platelet reactivity (HoTPR), and associated adverse cardiovascular outcomes in patients with peripheral arterial disease (PAD). BACKGROUND: The association of aspirin and clopidogrel HoTPR with outcomes in PAD remains unclear. METHODS: This is a prospective cohort study of patients with angiographically documented PAD involving carotid and lower extremity arteries...
February 7, 2018: Catheterization and Cardiovascular Interventions
https://www.readbyqxmd.com/read/29403303/the-clinicopathological-features-of-patients-with-membranous-nephropathy
#11
Hanyu Zhu, Qiuxia Han, Dong Zhang, Yong Wang, Jing Gao, Xiaoli Yang, Wenjia Geng, Xiangmei Chen
Background: Membranous nephropathy (MN) represents a distinct glomerular disease which has been considered as a major cause of nephrotic syndrome (NS) in adults. Evidences show that the clinicopathological features of MN are various among MN cases. This study aimed to summarize and analyze the clinicopathological features of patients with MN. Methods: A total of 231 MN patients were recruited in this study. Their clinical and pathological features were collected and analyzed according to their age, gender, pathological stages, and anti-phospholipase A2 receptor (anti-PLA2R) antibodies tests...
2018: International Journal of Nephrology and Renovascular Disease
https://www.readbyqxmd.com/read/29401359/anti-phospholipase-a2-receptor-antibodies-in-primary-membranous-nephropathy-10-key-points
#12
Emily P McQuarrie
No abstract text is available yet for this article.
February 1, 2018: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/29391226/antiparasitic-effects-induced-by-polyclonal-igy-antibodies-anti-phospholipase-a2-from-bothrops-pauloensis-venom
#13
Isabela Pacheco Borges, Mariana Ferreira Silva, Fernanda Maria Santiago, Lucas Silva de Faria, Álvaro Ferreira Júnior, Rafaela José da Silva, Mônica Costa Soares, Vitor de Freitas, Kelly Aparecida Geraldo Yoneyama, Eloísa Amália Vieira Ferro, Daiana Silva Lopes, Renata Santos Rodrigues, Veridiana de Melo Rodrigues
Activities of phospholipases (PLAs) have been linked to pathogenesis in various microorganisms, and implicated in cell invasion and so the interest in these enzymes as potential targets that could contribute to the control of parasite survival and proliferation. Chicken eggs immunized with BnSP-7, a Lys49 phospholipase A2 (PLA2) homologue from Bothrops pauloensis snake venom, represent an excellent source of polyclonal antibodies with potential inhibitory activity on parasite PLAs. Herein, we report the production, characterization and anti-parasitic effect of IgY antibodies from egg yolks of hens immunized with BnSP-7...
January 29, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29391000/computational-investigations-of-physicochemical-pharmacokinetic-toxicological-properties-and-molecular-docking-of-betulinic-acid-a-constituent-of-corypha-taliera-roxb-with-phospholipase-a2-pla2
#14
Mohammad Firoz Khan, Nusrat Nahar, Ridwan Bin Rashid, Akhtaruzzaman Chowdhury, Mohammad A Rashid
BACKGROUND: Betulinic acid (BA) is a natural triterpenoid compound and exhibits a wide range of biological and medicinal properties including anti-inflammatory activity. Therefore, this theoretical investigation is performed to evaluate (a) physicochemical properties such as acid dissociation constant (pKa), distribution coefficient (logD), partition coefficient (logP), aqueous solubility (logS), solvation free energy, dipole moment, polarizability, hyperpolarizability and different reactivity descriptors, (b) pharmacokinetic properties like human intestinal absorption (HIA), cellular permeability, skin permeability (PSkin), plasma protein binding (PPB), penetration of the blood brain barrier (BBB), (c) toxicological properties including mutagenicity, carcinogenicity, risk of inhibition of hERG gene and (d) molecular mechanism of anti-inflammatory action which will aid the development of analytical method and the synthesis of BA derivatives...
February 2, 2018: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/29390564/the-associations-of-stroke-transient-ischemic-attack-and-or-stroke-related-recurrent-vascular-events-with-lipoprotein-associated-phospholipase-a2-a-systematic-review-and-meta-analysis
#15
Ye Tian, Huan Jia, Sichen Li, Yanmin Wu, Li Guo, Guojun Tan, Bin Li
BACKGROUND: Studies on stroke and lipoprotein-associated phospholipase A2 (Lp-PLA2) have produced conflicting results. OBJECTIVE: The aim of the study was to assess the associations of Lp-PLA2 levels (mass and activity) with recurrent vascular events in patients with transient ischemic attack (TIA) and/or first ischemic stroke and with stroke in the general population. METHODS: The MEDLINE, Embase, the Cochrane Library, Web of Science, Science Direct, China National Knowledge Infrastructure, China Biology Medical Disc (CBMdisc), and WanFang were searched for prospective observational studies reported until January 2017...
December 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29388171/combined-membranous-nephropathy-and-tubulointerstitial-nephritis-as-a-rare-renal-manifestation-of-igg4-related-disease-a-case-based-literature-review
#16
Wei Zhang, Jeffrey H Glaze, David Wynne
IgG4-related disease (IgG4-RD) is a newly recognized immune-mediated multisystemic disease characterized by a fibro-inflammatory condition with tissue infiltration of IgG4-positive plasma cells and often associated with elevated serum IgG4 levels. Typical renal involvement of IgG4-RD presents as tubulointerstitial nephritis (TIN), membranous or membranoproliferative nephropathy. We are presenting a case with combined IgG4 membranous nephropathy and TIN, as well as a literature review on pathophysiology, diagnosis and treatment of IgG4-RD...
February 1, 2018: CEN Case Reports
https://www.readbyqxmd.com/read/29386447/-underlying-mechanisms-of-methamphetamine-induced-self-injurious-behavior-and-lethal-effects-in-mice
#17
Tomohisa Mori, Toshiko Sawaguchi
Relatively high doses of psychostimulants induce neurotoxicity on the dopaminergic system and self-injurious behavior (SIB) in rodents. However the underlying neuronal mechanisms of SIB remains unclear. Dopamine receptor antagonists, N-methyl-D-aspartic acid (NMDA) receptor antagonists, Nitric Oxide Synthase (NOS) inhibitors and free radical scavengers significantly attenuate methamphetamine-induced SIB. These findings indicate that activation of dopamine as well as NMDA receptors followed by radical formation and oxidative stress, especially when mediated by NOS activation, is associated with methamphetamine-induced SIB...
2018: Nihon Eiseigaku Zasshi. Japanese Journal of Hygiene
https://www.readbyqxmd.com/read/29374817/differential-effects-of-spla2-gv-and-gx-on-cellular-proliferation-and-lipid-accumulation-in-ht29-colon-cancer-cells
#18
Wei Hsum Yap, Su Wen Phang, Nafees Ahmed, Yang Mooi Lim
Secretory phospholipase A2 (sPLA2) group of enzymes have been shown to hydrolyze phospholipids, among which sPLA2 Group V (GV) and Group X (GX) exhibit high selectivity towards phosphatidylcholine-rich cellular plasma membranes. The enzymes have recently emerged as key regulators in lipid droplets formation and it is hypothesized that sPLA2-GV and GX enhanced cell proliferation and lipid droplet accumulation in colon cancer cells (HT29). In this study, cell viability and lipid droplet accumulation were assessed by Resazurin assay and Oil-Red-O staining...
January 27, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29371621/molecular-mechanism-of-resveratrol-s-lipid-membrane-protection
#19
Qinqin Fei, David Kent, Wesley M Botello-Smith, Fariah Nur, Saadia Nur, Abdelaziz Alsamarah, Payal Chatterjee, Maria Lambros, Yun Luo
Resveratrol, a natural compound found in red wine and various vegetables, has drawn increasing interest due to its reported benefit in cardiovascular protection, neurodegenerative disorders, and cancer therapy. The mechanism by which resveratrol exerts such pleiotropic effects remains unclear. It remains as one of the most discussed polyphenol compounds in the debating "French Paradox". In this study, using molecular dynamics simulations of dipalmitoyl phosphatidylcholine (DPPC) bilayer with resveratrol, we generated a free energy map of resveratrol's location and orientation of inside the lipid bilayer...
January 25, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29366279/secretory-phospholipase-a2-modified-high-density-lipoprotein-potently-suppresses-platelet-activation
#20
Gunther Marsche, Michael Holzer, Sanja Curcic
No abstract text is available yet for this article.
August 2017: Atherosclerosis
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