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https://www.readbyqxmd.com/read/28739210/bile-salt-stimulated-lipase-inhibition-by-phospholipids-and-relief-by-phospholipase-a2
#1
Elena Venuti, Dmitry Shishmarev, Philip W Kuchel, Shoma Dutt, Caron S Blumenthal, Kevin J Gaskin
INTRODUCTION: Bile salt stimulated lipase (BSSL; Enzyme Commission (EC) number 3.1.1.13) has been a candidate triglyceridase for improving enzyme therapy for pancreatic insufficiency; however, its efficacy is near absent. We hypothesise that similarly to pancreatic lipase, BSSL is inhibited by phospholipids and this inhibition is relieved by Phospholipase A2 (PLA2; EC 3.1.1.4), and the present study was undertaken to explore this possibility. MATERIALS AND METHODS: Synthetic emulsions of triglyceride and phosphatidylcholine (PC) or lysophosphatidylcholine (LPC)/bile salt mixed micelles were used as a model of intestinal digestion-media...
July 21, 2017: Journal of Cystic Fibrosis: Official Journal of the European Cystic Fibrosis Society
https://www.readbyqxmd.com/read/28738484/-correlation-between-serum-inflammatory-cytokine-levels-and-fibrous-cap-thickness-of-fibrofatty-plaque-in-coronary-culprit-lesions
#2
Y Zhong, F Ye, W You, Z M Wu
Objective: To identify the correlation between serum inflammatory cytokine levels including high sensitive C reactive protein (hs-CRP) and lipoprotein associated phospholipase (Lp-PLA2) and the fibrous cap thickness of fibrofatty plaque in coronary culprit lesions. Methods: Clinical data of 117 patients with selective coronary artery angiography diagnosed coronary artery disease admitted to our hospital from January 2015 to January 2016 were retrospective analyzed. According to type of coronary disease, patients were divided into 3 subgroups: SAP group (containing 47 stable angina patients), UAP group (containing 52 unstable angina patients), and NSTEMI group(containing 18 acute non ST segment elevation myocardial infarction patients)...
July 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/28731347/integrated-venomics-and-venom-gland-transcriptome-analysis-of-juvenile-and-adult-mexican-rattlesnakes-crotalus-simus-c-tzabcan-and-c-culminatus-revealed-mirna-modulated-ontogenetic-shifts
#3
Jordi Durban, Libia Sanz, Dilza Trevisan-Silva, Edgar Neri-Castro, Alejandro Alagón, Juan Jose Calvete
Adult rattlesnakes within genus Crotalus express one of two distinct venom phenotypes, type I (haemorrhagic) and type II (neurotoxic). In Costa Rican Central American rattlesnake, ontogenetic changes in the concentration of miRNAs modulate venom type II to type I transition. Venomics and venom gland transcriptome analyses showed that adult C. simus and C. tzabcan expressed intermediate patterns between type II and type I venoms, whereas C. culminatus had a canonical type I venom. Neonate/juvenile and adult Mexican rattlesnakes showed notable inter- and intraspecific variability in the number, type, abundance and ontogenetic shifts of the transcriptional and translational venom gland activities...
July 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28728446/hpma-copolymer-phospholipase-c-and-dextrin-phospholipase-a2-as-model-triggers-for-polymer-enzyme-liposome-therapy-pelt
#4
Elaine L Ferguson, Anna Scomparin, Hanna Hailu, Ronit Satchi-Fainaro
'Polymer Enzyme Liposome Therapy' (PELT) is a two-step anticancer approach in which a liposomal drug and polymer-phospholipase conjugate are administered sequentially to target the tumour interstitium by the enhanced permeability and retention effect, and trigger rapid, local, drug release. To date, however, the concept has only been described theoretically. We synthesised two polymer conjugates of phospholipase C (PLC) and A2 (PLA2) and evaluated their ability to trigger anthracycline release from the clinically-used liposomes, Caelyx® and DaunoXome(®)...
July 21, 2017: Journal of Drug Targeting
https://www.readbyqxmd.com/read/28726649/lipoprotein-associated-phospholipase-a2-as-a-marker-of-vulnerable-atherosclerotic-plaque-in-patients-with-internal-carotid-artery-stenosis
#5
P Štefanič, I Kopolovets, Z Hertelyová, Š Tóth, M Frankovičová
The aim of this study was to compare the concentration of inflammatory vascular markers and morphological structure of atherosclerotic plaque in symptomatic and asymptomatic patients with the stenosis of internal carotid artery (ICA). The research was carried out in 70 patients with hemodynamically significant stenosis of ICA out of which 40 (57%) were asymptomatic patients and 30 (43%) were symptomatic patients, of which 20 patients (66%) have had a stroke, or transient ischemic attack (TIA), 10 patients (33%)...
June 2017: Georgian Medical News
https://www.readbyqxmd.com/read/28715731/metabolomics-analysis-reveals-that-benzo-a-pyrene-a-component-of-pm2-5-promotes-pulmonary-injury-by-modifying-lipid-metabolism-in-a-phospholipase-a2-dependent-manner-in-vivo-and-in-vitro
#6
Song-Yang Zhang, Danqing Shao, Huiying Liu, Juan Feng, Baihuan Feng, Xiaoming Song, Qian Zhao, Ming Chu, Changtao Jiang, Wei Huang, Xian Wang
Particulate matter with an aerodynamic diameter less than 2.5μM (PM2.5) is one of the major environmental pollutants in China. In this study, we carried out a metabolomics profile study on PM2.5-induced inflammation. PM2.5 from Beijing, China, was collected and given to rats through intra-tracheal instillation in vivo. Acute pulmonary injury were observed by pulmonary function assessment and H.E. staining. The lipid metabolic profile was also altered with increased phospholipid and sphingolipid metabolites in broncho-alveolar lavage fluid (BALF) after PM2...
July 4, 2017: Redox Biology
https://www.readbyqxmd.com/read/28712914/ige-cross-reactivity-of-phospholipase-a2-and-hyaluronidase-of-apis-dorsata-giant-asian-honeybee-and-apis-mellifera-western-honeybee-venom-possible-use-of-a-%C3%A2-mellifera-venom-for-diagnosis-of-patients-allergic-to-a-%C3%A2-dorsata-venom
#7
Peshala Gunasekara, S M Handunnetti, Sunil Premawansa, E W R A Witharana, W M D K Dasanayake, Indra P Ratnayake, Suranjith L Seneviratne, R K S Dias, G A S Premakumara, Rajiva de Silva
Diagnostic and therapeutic reagents are unavailable for anaphylaxis arising from stings by Apis dorsata. Venom profiles and cross-reactivity of A. dorsata and Apis mellifera were compared, to ascertain whether venom of A. mellifera can be used for diagnosis in A. dorsata allergy. Both venom profiles were similar by High Performance Liquid Chromatography and SDS-PAGE. Sera of 29 of 30 (96.7%) patients with anaphylaxis to A. dorsata stings had IgE to the phospholipase-2 (PLA2) doublet (15 and 16 kDa) of A...
July 14, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28710425/naja-sputatrix-venom-preconditioning-attenuates-neuroinflammation-in-a-rat-model-of-surgical-brain-injury-via-pla2-5-lox-ltb4-cascade-activation
#8
Yuechun Wang, Prativa Sherchan, Lei Huang, Onat Akyol, Devin W McBride, John H Zhang
Inflammatory preconditioning is a mechanism in which exposure to small doses of inflammatory stimuli prepares the body against future massive insult by activating endogenous protective responses. Phospholipase A2/5-lipoxygenase/leukotriene-B4 (PLA2/5-LOX/LTB4) axis is an important inflammatory signaling pathway. Naja sputatrix (Malayan spitting cobra) venom contains 15% secretory PLA2 of its dry weight. We investigated if Naja sputatrix venom preconditioning (VPC) reduces surgical brain injury (SBI)-induced neuroinflammation via activating PLA2/5-LOX/LTB4 cascade using a partial frontal lobe resection SBI rat model...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28708892/cross-recognition-of-a-pit-viper-crotalinae-polyspecific-antivenom-explored-through-high-density-peptide-microarray-epitope-mapping
#9
Mikael Engmark, Bruno Lomonte, José María Gutiérrez, Andreas H Laustsen, Federico De Masi, Mikael R Andersen, Ole Lund
Snakebite antivenom is a 120 years old invention based on polyclonal mixtures of antibodies purified from the blood of hyper-immunized animals. Knowledge on antibody recognition sites (epitopes) on snake venom proteins is limited, but may be used to provide molecular level explanations for antivenom cross-reactivity. In turn, this may help guide antivenom development by elucidating immunological biases in existing antivenoms. In this study, we have identified and characterized linear elements of B-cell epitopes from 870 pit viper venom protein sequences by employing a high-throughput methodology based on custom designed high-density peptide microarrays...
July 14, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28704853/rbp4-lp-pla2-netrin-1-signaling-regulation-of-cognitive-dysfunction-in-diabetic-nephropathy-complicated-with-silent-cerebral-infarction
#10
Danyan Chen, Xiaolong Huang, Song Lu, Huacong Deng, Hua Gan, Xiaogang Du, Tang Weixue
Aim To investigate the RBP4/Lp-PLA2/Netrin-1 signal regulation of cognitive function impairment in diabetic nephropathy patients with silent cerebral infarction (SCI). Methods One-hundred patients newly diagnosed with diabetic nephropathy patients were included. The patients were divided into SCI group and NSCI group according to the radiological data. The degrees of cognitive dysfunction were evaluated. Serum RBP4 concentrations were determined by ELISA and protein expressions of Lp-PLA2 and Netrin-1 were determined by Western Blot...
July 13, 2017: Experimental and Clinical Endocrinology & Diabetes
https://www.readbyqxmd.com/read/28698554/chondroprotection-by-urocortin-involves-blockade-of-the-mechanosensitive-ion-channel-piezo1
#11
K M Lawrence, R C Jones, T R Jackson, R L Baylie, B Abbott, B Bruhn-Olszewska, T N Board, I C Locke, S M Richardson, P A Townsend
Osteoarthritis (OA) is characterised by progressive destruction of articular cartilage and chondrocyte cell death. Here, we show the expression of the endogenous peptide urocortin1 (Ucn1) and two receptor subtypes, CRF-R1 and CRF-R2, in primary human articular chondrocytes (AC) and demonstrate its role as an autocrine/paracrine pro-survival factor. This effect could only be removed using the CRF-R1 selective antagonist CP-154526, suggesting Ucn1 acts through CRF-R1 when promoting chondrocyte survival. This cell death was characterised by an increase in p53 expression, and cleavage of caspase 9 and 3...
July 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28688916/venom-proteome-of-the-yellow-lipped-sea-krait-laticauda-colubrina-from-bali-insights-into-subvenomic-diversity-venom-antigenicity-and-cross-neutralization-by-antivenom
#12
Choo Hock Tan, Kin Ying Wong, Kae Yi Tan, Nget Hong Tan
The venom proteome of Laticauda colubrina (Bali, Indonesia) was elucidated by nano-ESI-LCMS/MS of the venom reverse-phase HPLC fractions. Altogether 31 distinct forms of proteins were identified and clustered into three toxin families: three-finger toxin (3FTX, 66.12% of total venom proteins), phospholipase A2 (PLA2, 33.26%) and cysteine-rich secretory protein (CRiSP, 0.05%). The 3FTX were α-neurotoxins (five long neurotoxins, LNTX, 48.87%; two short neurotoxins, SNTX, 16.70%) and a trace amount of two cytotoxins (CTX, 0...
July 5, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28680124/refining-long-term-prediction-of-cardiovascular-risk-in-diabetes-the-vildia-score
#13
Georg Goliasch, Günther Silbernagel, Marcus E Kleber, Tanja B Grammer, Stefan Pilz, Andreas Tomaschitz, Philipp E Bartko, Gerald Maurer, Wolfgang Koenig, Alexander Niessner, Winfried März
Cardiovascular risk assessment in patients with diabetes relies on traditional risk factors. However, numerous novel biomarkers have been found to be independent predictors of cardiovascular disease, which might significantly improve risk prediction in diabetic patients. We aimed to improve prediction of cardiovascular risk in diabetic patients by investigating 135 evolving biomarkers. Based on selected biomarkers a clinically applicable prediction algorithm for long-term cardiovascular mortality was designed...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28673363/a-red-yeast-rice-olive-extract-supplement-reduces-biomarkers-of-oxidative-stress-oxldl-and-lp-pla2-in-subjects-with-metabolic-syndrome-a-randomised-double-blind-placebo-controlled-trial
#14
Nina Hermans, Anastasia Van der Auwera, Annelies Breynaert, Annelies Verlaet, Tess De Bruyne, Luc Van Gaal, Luc Pieters, Veronique Verhoeven
BACKGROUND: Metabolic syndrome (MetS) refers to clustered cardiovascular risk factors (abdominal obesity, pre-diabetes, high blood pressure, dyslipidaemia). Therapies targeting oxidative stress may delay progression to atherosclerosis and diabetes. We investigated the anti-oxidative effect of a supplement combining red yeast rice and olive extract in patients with MetS. METHODS: A double-blind, placebo-controlled, randomised trial was conducted with 50 patients with MetS as defined by National Cholesterol Education Program Adult Treatment Panel III criteria...
July 3, 2017: Trials
https://www.readbyqxmd.com/read/28672255/lipidomics-to-investigate-the-pharmacologic-mechanisms-of-ginkgo-folium-in-the-hyperuricemic-rat-model
#15
Shuli Zhang, Jingcong Zhuang, Guijuan Yue, Yiping Wang, Mingyu Liu, Bing Zhang, Zhenxia Du, Qun Ma
Hyperuricemia caused by purine metabolic abnormalities is reported to have close correlation with lipid metabolic disorders. Ginkgo folium, a frequently-used lipid-lowering medicine, has significant anti-hyperuricemia effects. However, it is poorly known about the interaction between lowering uric acid and regulation of lipid metabolic disorders. In this study, hyperuricemic rat model was induced by orally administration with fructose. Ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) combined with pattern recognition approaches were used to determine different lipid metabolites in serum of control group, model group, and different doses of ginkgo folium groups...
June 20, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28669075/origin-and-evolution-of-group-xi-secretory-phospholipase-a2-from-flax-linum-usitatissimum-based-on-phylogenetic-analysis-of-conserved-domains
#16
Payal Gupta, Raman Saini, Prasanta K Dash
Phospholipase A2 (PLA2) belongs to class of lipolytic enzymes (EC 3.1.1.4). Lysophosphatidic acid (LPA) and free fatty acids (FFAs) are the products of PLA2 catalyzed hydrolysis of phosphoglycerides at sn-2 position. LPA and FFA that act as second mediators involved in the development and maturation of plants and animals. Mining of flax genome identified two phospholipase A2 encoding genes, viz., LusPLA 2 I and LusPLA 2 II (Linum usitatissimum secretory phospholipase A2). Molecular simulation of LusPLA2s with already characterized plant sPLA2s revealed the presence of conserved motifs and signature domains necessary to classify them as secretory phospholipase A2...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28656267/epoxyeicosatrienoic-acid-ameliorates-cerebral-ischemia-reperfusion-injury-by-inhibiting-inflammatory-factors-and-pannexin-1
#17
Zhigang Liu, Yongfang Liu, Haixiao Zhou, Xiangyun Fu, Gang Hu
Epoxyeicosatrienoic acid (EET) has wide applications due to the unique biological effects of anti‑hyperlipidemia, inhibition of platelet aggregation, anti‑inflammation, anti‑cancer, anti‑lipid oxidation and the promotion of brain tissue development. The present study investigated whether EET ameliorates cerebral ischemia‑reperfusion injury (CIRI) by inhibiting inflammatory factors and pannexin. Specific pathogen‑free 7‑week‑old male Sprague‑Dawley rats were randomly divided into three groups: Sham, CIRI and EET...
August 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28651842/sulodexide-prevents-activation-of-the-pla2-cox-2-vegf-inflammatory-pathway-in-human-retinal-endothelial-cells-by-blocking-the-effect-of-age-rage
#18
Giovanni Giurdanella, Francesca Lazzara, Nunzia Caporarello, Gabriella Lupo, Carmelina Daniela Anfuso, Chiara M Eandi, Gian Marco Leggio, Filippo Drago, Claudio Bucolo, Salvatore Salomone
Diabetic retinopathy is characterized by the breakdown of endothelial blood-retinal barrier. We tested the hypothesis that sulodexide (SDX), a highly purified glycosaminoglycan composed of 80% iduronylglycosaminoglycan sulfate and 20% dermatan sulfate, protects human retinal endothelial cells (HREC) from high glucose (HG)-induced damage, through the suppression of inflammatory ERK/cPLA2/COX-2/PGE2 pathway, by blocking the effect of advanced glycation end-products (AGEs). HREC were treated with HG (25mM) or AGEs (glycated-BSA, 2mg/ml) for 48h, with or without SDX (60μg/ml) or aflibercept (AFL, 40μg/ml), a VEGF-trap...
June 23, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28651698/an-association-between-pla2g6-and-pla2g4c-gene-polymorphisms-and-schizophrenia-risk-and-illness-severity-in-a-croatian-population
#19
Sergej Nadalin, Alena Buretić-Tomljanović
We investigated the allele and genotype frequency of the rs4375 and rs1549637 polymorphisms in phospholipase A2 (PLA2)G6 and PLA2G4C genes in 203 patients with schizophrenia and 191 controls in a Croatian population. We hypothesized that these polymorphic variations might influence the age of schizophrenia onset and Positive and Negative Syndrome Scale psychopathology (PANSS) data. We detected a significant overrepresentation of the PLA2G6-CT and PLA2G4C-AT genotype combination in patients compared with controls (14...
June 2017: Prostaglandins, Leukotrienes, and Essential Fatty Acids
https://www.readbyqxmd.com/read/28644968/serum-lipoprotein-associated-phospholipase-a2-predicts-the-formation-of-carotid-artery-plaque-and-its-vulnerability-in-anterior-circulation-cerebral-infarction
#20
Yuping Yang, Tao Xue, Juehua Zhu, Jiayi Xu, Xiaowei Hu, Penghao Wang, Tao Kong, Yan Yan, Lihui Yang, Shouru Xue
OBJECTIVE: Circulation inflammation markers such as high-sensitive C-reactive protein (hsCRP) and lipoprotein-associated phospholipase A2 (Lp-PLA2) are considered as predictors of cerebral and cardiac vascular diseases. However, the role of hsCRP and Lp-PLA2 in the anterior circulation cerebral infarction (ACI) is to be elaborated. PATIENTS AND METHODS: We included 100 patients with acute anterior circulation cerebral infarction (AaCI group) and 50 non-infarction subjects (control group)...
June 12, 2017: Clinical Neurology and Neurosurgery
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