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https://www.readbyqxmd.com/read/29780521/translating-molecular-detections-into-a-simple-temperature-test-using-a-target-responsive-smart-thermometer
#1
Jingjing Zhang, Hang Xing, Yi Lu
While it has been well recognized that affordable and pocket-size devices play a major role in environmental monitoring, food safety and medical diagnostics, it often takes a tremendous amount of resources to develop such devices. Devices that have been developed are often dedicated devices that can detect only one or a few targets. To overcome these limitations, we herein report a novel target-responsive smart thermometer for translating molecular detection into a temperature test. The sensor system consists of a functional DNA-phospholipase A2 (PLA2 ) enzyme conjugate, a liposome-encapsulated NIR dye, and a thermometer interfaced with a NIR-laser device...
April 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29765452/effects-and-potential-mechanism-of-atorvastatin-treatment-on-lp-pla2-in-rats-with-dyslipidemia
#2
Dongdan Zheng, Anping Cai, Rulin Xu, Zhuocheng Mai, Yingling Zhou, Fanfang Zeng, Liwen Li, Weiyi Mai
Introduction: The effects of statins on lipoprotein-associated phospholipase A2 (Lp-PLA2) are controversial, and the present study aimed to investigate whether atorvastatin could reduce Lp-PLA2 in rats with dyslipidemia. Material and methods: A high-fat and high-cholesterol diet was prescribed to produce a dyslipidemia model. Thereafter, low-dose atorvastatin (5 mg/kg/day), high-dose atorvastatin (20 mg/kg/day) or saline (without-treatment group) was prescribed for 14 days...
April 2018: Archives of Medical Science: AMS
https://www.readbyqxmd.com/read/29751071/protective-effects-of-a-natural-herbal-compound-quercetin-against-snake-venom-induced-hepatic-and-renal-toxicities-in-rats
#3
Abdulrahman K Al-Asmari, Haseeb A Khan, Rajamohamed A Manthiri, Ahmad A Al-Khlaiwi, Bayan A Al-Asmari, Khalid E Ibrahim
Echis pyramidum is a highly poisonous viper snake. Previous studies have shown acute phase hepatic and renal toxicities of Echis pyramidum venom (EPV) in rats. This study reports the protective effects of a natural herbal compound quercetin (QRC) on EPV-induced hepatic and renal toxicities in rats. A singly injection of EPV (4.76 mg/kg) caused significant increase in serum biomarkers of liver and kidney function. Pre-treatment of QRC (10 mg/kg) significantly reduced the toxic effects of EPV on functional impairment in liver and kidneys of rats...
May 8, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29737567/impact-of-a-non-synonymous-q281r-polymorphism-on-structure-of-human-lipoprotein-associated-phospholipase-a-2-lp-pla-2
#4
Arun B Gurung, Atanu Bhattacharjee
Non-synonymous single nucleotide polymorphisms (nsSNPs) are genetic variations at single base resulting in an amino acid change which have been associated with various complex human diseases. The human Lipoprotein-associated phospholipase A2 (Lp-PLA2 ) gene harbours a rare Q281R polymorphism which was previously reported to cause loss of enzymatic function. Lp-PLA2 is an important enzyme which catalyzes the hydrolysis of polar phospholipids releasing pro-atherogenic and pro-inflammatory mediators involved in the pathogenesis of atherosclerosis...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29729992/proteomics-functional-characterization-and-antivenom-neutralization-of-the-venom-of-pakistani-russell-s-viper-daboia-russelii-from-the-wild
#5
Tasnim Faisal, Kae Yi Tan, Si Mui Sim, Naeem Quraishi, Nget Hong Tan, Choo Hock Tan
The venom proteome of wild Pakistani Russell's viper (Daboia russelii) was investigated through nano-ESI-LCMS/MS of the reverse-phase HPLC fractions. A total of 54 venom proteins were identified and clustered into 11 protein families. Phospholipase A2 (PLA2 , 63.8%) and Kunitz-type serine protease inhibitor (KSPI, 16.0%) were most abundant, followed by snake venom serine protease (SVSP, 5.5%, mainly Factor V activating enzyme), vascular endothelial growth factor (VEGF, 4.3%), snake venom metalloprotease (SVMP, 2...
May 3, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29715185/darapladib-inhibits-atherosclerosis-development-in-type-2-diabetes-mellitus-sprague-dawley-rat-model
#6
Titin Andri Wihastuti, Teuku Heriansyah, Hanifa Hanifa, Sri Andarini, Zuhrotus Sholichah, Yuni Hendrati Sulfia, Aditya Angela Adam, Jeki Refialdinata, Nurul Cholifah Lutfiana
OBJECTIVE: Increase in the low-density lipoprotein (LDL) level in diabetes mellitus and atherosclerosis is related to lipoprotein associated phospholipase A2 (Lp-PLA2). Lp-PLA2 is an enzyme that produces lysophosphatidylcholine (LysoPC) and oxidized nonesterified fatty acids (oxNEFA). LysoPC regulates inflammation mediators, including intra-cellular adhesion molecule-1 (ICAM-1). Darapladib is known as a Lp-PLA2 specific inhibitor. The aim of this study was to reveal the effect of darapladib on the foam cell number, inducible nitric oxide synthase (iNOS), and ICAM-1 expression in aorta at early stages of the atherosclerosis in type 2 diabetes mellitus Sprague-Dawley rat model...
April 1, 2018: Endocrine Regulations
https://www.readbyqxmd.com/read/29710018/heat-stress-affects-prostaglandin-synthesis-in-bovine-endometrial-cells
#7
Shunsuke Sakai, Natsumi Hagihara, Mariko Kuse, Koji Kimura, Kiyoshi Okuda
Heat stress (HS) negatively affects reproduction in cattle; however, its effect on endocrine function in bovine endometrial cells remains unclear. In this study, we examined the effects of HS on the production of prostaglandin (PG)E2 and PGF2α in the cultured bovine endometrial epithelial and stromal cells separately. To evaluate the effect of HS on endocrine function, the cells were cultured at 38.5°C (control) or 40.5°C (HS). After treatment, PGE2 and PGF2α levels were measured via enzyme immunoassay (EIA) and mRNA expressions of enzymes involved in PG synthesis were examined via quantitative reverse transcription polymerase chain reaction (RT-PCR)...
April 30, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29709480/effects-of-enzymes-on-elastic-modulus-of-low-density-lipoproteins-were-investigated-using-atomic-force-microscopy
#8
Seiji Takeda, Toshihiro Sakurai, Shu-Ping Hui, Hirotoshi Fuda, Hitoshi Chiba
Oxidation of low-density lipoproteins (LDLs) induces development of cardiovascular disease. Recently, reports of studies using atomic force microscopy (AFM) have described that the elastic modulus of metal-induced oxidized LDLs is lower than the modulus before oxidation. However, the mechanisms of change of the elastic modulus have not been well investigated. We postulated that disorder of the LDL structure might decrease the elastic modulus. This study measured the elastic modulus of LDLs before and after enzyme treatment with V8 protease, α-chymotrypsin, and phospholipase A2 ...
April 27, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29709018/phospholipase-a2-activity-during-the-replication-cycle-of-the-flavivirus-west-nile-virus
#9
Susann Liebscher, Rebecca L Ambrose, Turgut E Aktepe, Andrea Mikulasova, Julia E Prier, Leah K Gillespie, Adam J Lopez-Denman, Thusitha W T Rupasinghe, Dedreia Tull, Malcolm J McConville, Jason M Mackenzie
Positive-sense RNA virus intracellular replication is intimately associated with membrane platforms that are derived from host organelles and comprised of distinct lipid composition. For flaviviruses, such as West Nile virus strain Kunjin virus (WNVKUN) we have observed that these membrane platforms are derived from the endoplasmic reticulum and are rich in (at least) cholesterol. To extend these studies and identify the cellular lipids critical for WNVKUN replication we utilized a whole cell lipidomics approach and revealed an elevation in phospholipase A2 (PLA2) activity to produce lyso-phosphatidylcholine (lyso-PChol)...
April 30, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/29705150/a-myotoxic-lys49-phospholipase-a-2-homologue-is-the-major-component-of-the-venom-of-bothrops-cotiara-from-misiones-argentina
#10
Adolfo de Roodt, Julián Fernández, Daniela Solano, Bruno Lomonte
Bothrops cotiara is a pitviper found in Southeastern Brazil and, scarcely, in the Misiones province of Argentina. In contrast to considerable information available on the venom of the Brazilian snake population, that of Misiones has received little attention. While exploring the chromatographic venom profile of Argentinean B. cotiara, a major protein peak was found which, according to a previous study, is not present in the venom of Brazilian origin. The corresponding protein was isolated by RP-HPLC, and characterized by electrophoresis, mass spectrometry, phospholipase A2 (PLA2 ) assay, and myotoxic activities...
April 26, 2018: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/29702100/signaling-pathways-activated-by-resolvin-e1-to-stimulate-mucin-secretion-and-increase-intracellular-ca-2-in-cultured-rat-conjunctival-goblet-cells
#11
Marit Lippestad, Robin R Hodges, Tor P Utheim, Charles N Serhan, Darlene A Dartt
Glycoconjugate mucin secretion from conjunctival goblet cells is tightly regulated by nerves and specialized pro-resolving mediators (SPMs) to maintain ocular surface health. Here we investigated the actions of the SPM resolvin E1 (RvE1) on cultured rat conjunctival goblet cell glycoconjugate secretion and intracellular [Ca2+ ] ([Ca2+ ]i ) and the signaling pathways used by RvE1. Goblet cells were cultured from rat conjunctiva in RPMI medium. The amount of RvE1-stimulated glycoconjugate mucin secretion was determined using an enzyme-linked lectin assay with Ulex Europaeus Agglutinin 1 lectin...
April 24, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29701904/trpv4-activates-cytosolic-phospholipase-a-2-via-ca-2-dependent-pkc-erk1-2-signaling-in-controlling-hypertensive-contraction
#12
Hongjuan Li, Hao Kan, Xiaodong Zhang, Zhenyu Yang, Jian Jin, Peng Zhang, Xin Ma
Activation of TRPV4 (transient receptor potential vanilloid 4) has been reported to result in endothelium-dependent contraction in the aortae of hypertensive mice. This contraction involved increased cPLA2 (cytosolic phospholipase A2 ) activity. The mechanism by which TRPV4 regulates cPLA2 activity to induce contraction in hypertension, however, is unknown. Through measurements of arterial tension and protein level, we showed that high salt diet induced hypertension increases activity of PKC (protein kinase C) and ERK1/2 (extracellular signal-regulated kinase 1/2)...
April 27, 2018: Clinical and Experimental Pharmacology & Physiology
https://www.readbyqxmd.com/read/29692802/hemolytic-anticancer-and-antigiardial-activity-of-palythoa-caribaeorum-venom
#13
Fernando Lazcano-Pérez, Ariana Zavala-Moreno, Yadira Rufino-González, Martha Ponce-Macotela, Alejandro García-Arredondo, Miguel Cuevas-Cruz, Saúl Gómez-Manzo, Jaime Marcial-Quino, Barbarín Arreguín-Lozano, Roberto Arreguín-Espinosa
Background: Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis ...
2018: Journal of Venomous Animals and Toxins Including Tropical Diseases
https://www.readbyqxmd.com/read/29690731/-association-of-serum-lipoprotein-associated-phospholipase-a2-with-vascular-dementia-after-ischemic-stroke
#14
Q L Zhang, P Lu, J W Zhang
Objective: To study the relationship of serum lipoprotein-associated phospholipase A2 (Lp-PLA2) with vascular dementia (VD) after ischemic stroke. Methods: A total of 226 ischemic stroke patients who visited the People's Hospital of Zhengzhou University from June.2013 to Oct.2016 were included and divided into the VD group and the non-dementia group according to the degree of cognitive impairment.The non-dementia group were further divided into the normal cognition group and the vascular cognitive impairment no dementia(VCIND) group...
April 17, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29675946/plasma-proteomics-identifies-a-chemokine-storm-in-idiopathic-multicentric-castleman-disease
#15
Sheila K Pierson, Aaron J Stonestrom, Dustin Shilling, Jason Ruth, Christopher S Nabel, Amrit Singh, Yue Ren, Katie Stone, Hongzhe Li, Frits van Rhee, David C Fajgenbaum
Human Herpesvirus-8 (HHV-8)-negative/idiopathic multicentric Castleman disease (iMCD) is a poorly understood disease involving polyclonal lymphoproliferation with dysmorphic germinal centers, constitutional symptoms, and multi-organ failure. Patients can experience thrombocytopenia, anasarca, reticulin fibrosis, renal dysfunction, organomegaly, and normal immunoglobulin levels, - iMCD-TAFRO. Others experience thrombocytosis, milder effusions, and hypergammaglobulinemia, -iMCD-Not Otherwise Specified (iMCD-NOS)...
April 20, 2018: American Journal of Hematology
https://www.readbyqxmd.com/read/29673602/a-comparison-of-accuracy-and-computational-feasibility-of-two-record-linkage-algorithms-in-retrieving-vital-status-information-from-hiv-aids-patients-registered-in-brazilian-public-databases
#16
Adelzon Assis de Paula, Denise Franqueira Pires, Pedro Alves Filho, Kátia Regina Valente de Lemos, Eduardo Barçante, Antonio Guilherme Pacheco
BACKGROUND AND OBJECTIVE: While cross-referencing information from people living with HIV/AIDS (PLWHA) to the official mortality database is a critical step in monitoring the HIV/AIDS epidemic in Brazil, the accuracy of the linkage routine may compromise the validity of the final database, yielding to biased epidemiological estimates. We compared the accuracy and the total runtime of two linkage algorithms applied to retrieve vital status information from PLWHA in Brazilian public databases...
June 2018: International Journal of Medical Informatics
https://www.readbyqxmd.com/read/29656017/proteomic-and-toxinological-characterization-of-the-venom-of-the-south-african-ringhals-cobra-hemachatus-haemachatus
#17
Andrés Sánchez, María Herrera, Mauren Villalta, Daniela Solano, Álvaro Segura, Bruno Lomonte, José María Gutiérrez, Guillermo León, Mariángela Vargas
The protein composition and toxinological profile of the venom of the African spitting elapid Hemachatus haemachatus (Ringhals) were characterized by bottom-up proteomics and functional in vitro and in vivo assays. Venom is composed of abundant three-finger toxins (3FTxs; 63.3%), followed by phospholipases A2 (PLA2 s; 22.8%), snake venom metalloproteinases (SVMPs 7.1%), cysteine-rich secretory proteins (CRISPs; 4.1%) and Kunitz type protease inhibitors (KTPIs; 1.5%). 3FTxs are the main responsible for lethality and myotoxicity in mice and in vitro anticoagulant activity...
April 12, 2018: Journal of Proteomics
https://www.readbyqxmd.com/read/29649452/serum-secretory-phospholipase-a2-group-ib-correlates-with-the-severity-of-membranous-nephropathy
#18
Weihao Li, Mingming Zhang, Yaping Guo, Xiaomei Liu, Xin Ji, Junhua Su, Zhiping Zhang, Feifei Zhang
BACKGROUND: Serum secretory phospholipase A2 group IB (sPLA2-IB) is involved in the pathological processes of membranous nephropathy (MN). To date, there is no large-scale study validating the usefulness of circulating sPLA2-IB in the follow-up of patients with MN. This study investigated the role of circulating sPLA2-IB in the evaluation of severity of MN. METHODS: A total of 158 patients with primary membranous nephropathy (pMN), 34 with secondary membranous nephropathy (sMN) and 53 healthy controls were enrolled...
April 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29623345/lp-pla-2-activity-is-associated-with-increased-risk-of-diabetic-retinopathy-a-longitudinal-disease-progression-study
#19
Moneeza K Siddiqui, Gwen Kennedy, Fiona Carr, Alexander S F Doney, Ewan R Pearson, Andrew D Morris, Toby Johnson, Megan M McLaughlin, Rachel E Williams, Colin N A Palmer
AIMS/HYPOTHESIS: The aim of the study was to examine the association between lipoprotein-associated phospholipase A2 (Lp-PLA2 ) activity levels and incident diabetic retinopathy and change in retinopathy grade. METHODS: This was a cohort study of diabetic participants with serum collected at baseline and routinely collected diabetic retinal screening data. Participants with type 2 diabetes from the GoDARTS (Genetics of Diabetes Audit and Research in Tayside Scotland) cohort were used...
April 6, 2018: Diabetologia
https://www.readbyqxmd.com/read/29621954/lpar2-and-lpar4-are-the-main-receptors-responsible-for-lpa-actions-in-ovarian-endometriotic-cysts
#20
Ilona Kowalczyk-Zieba, Izabela Woclawek-Potocka, Tomasz Wasniewski, Dorota Boruszewska, Katarzyna Grycmacher, Emilia Sinderewicz, Joanna Staszkiewicz, Slawomir Wolczynski
Endometriosis has been considered as an estrogen (E2)-dependent and progesterone (P4)-resistant disease. On the other hand, lysophosphatidic acid (LPA) has been suggested as a significant modulator of ovarian pathology, acting via both LPA levels and LPA receptor (LPAR) upregulation. Therefore, the objective of the present study was to evaluate LPA concentration as well as LPARs, autotaxin (ATX), and phospholipase A2 (PLA2) expression in ovarian endometriotic cysts and normal endometrium with correlation of the expression of E2 and P4 receptors in endometriotic cysts...
January 1, 2018: Reproductive Sciences
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