keyword
MENU ▼
Read by QxMD icon Read
search

Biosafety

keyword
https://www.readbyqxmd.com/read/28731201/a-technological-and-regulatory-outlook-on-crispr-crop-editing
#1
Rea Globus, Udi Qimron
Generating plants with increased yields while maintaining low production and maintenance costs is highly important since plants are the major food source for humans and animals, as well as important producers of chemicals, pharmaceuticals, and fuels. Gene editing approaches, particularly the CRISPR-Cas system, are the preferred methods for improving crops, enabling quick, robust, and accurate gene manipulation. Nevertheless, new breeds of genetically-modified crops have initiated substantial debates concerning their biosafety, commercial use, and regulation...
July 21, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28719818/insights-into-the-unique-functionality-of-inorganic-micro-nanoparticles-for-versatile-ultrasound-theranostics
#2
REVIEW
Xiaoqin Qian, Xiaoxia Han, Yu Chen
The clinical ultrasound (US)-based theranostic biomedicine suffers from the critical issue that traditional microbubbles (MBs) have lots of drawbacks such as low stability, large particle size, difficult structural control, etc. The unique composition, structure and functionality of inorganic micro/nanoplatforms have shown their great prospect for solving these critical issues and drawbacks of traditional organic MBs. This review summarizes and discusses the state-of-art development on exploring inorganic micro/nanoparticles for versatile US-based biomedical applications, ranging from US imaging, photoacoustic imaging, sonodynamic therapy, high intensity-focused US ablation and US-triggered chemotherapy...
July 11, 2017: Biomaterials
https://www.readbyqxmd.com/read/28712134/phenotypic-and-genotypic-characterisation-of-clinically-relevant-bacteria-isolated-from-dental-waste-and-waste-workers-hands-mucosas-and-coats
#3
Thaysa Leite Tagliaferri, Cristina Dutra Vieira, Maria Auxiliadora Roque de Carvalho, Luiz Carlos Duarte Ladeira, Paula Prazeres Magalhães, Luiz de Macêdo Farias, Simone Gonçalves Dos Santos
Infectious wastes are potential sources of pathogenic microorganisms, which may represent a risk to the professionals who manage them. In this study we aimed to characterize the infectious bacteria present in dental waste and waste workers. The dental waste produced over 24 hours was collected and waste workers were sampled by swabbing. Isolate resistance profiles were characterized by Vitek(®) and PCR and biofilm formation by Congo Red agar, string test and microtiter assay. To assess similarity between the waste and the workers' samples, a random amplified polymorphic DNA test was used...
July 16, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28710545/comparative-muscle-proteomics-phosphoproteomics-analysis-provides-new-insight-for-the-biosafety-evaluation-of-fat-1-transgenic-cattle
#4
Xiangbo Xin, Xinfeng Liu, Xin Li, Xiangbin Ding, Shuping Yang, Congfei Jin, Guangpeng Li, Hong Guo
The biosafety of fat-1 transgenic cattle has been a focus of our studies since the first fat-1 transgenic cow was born. In this study, we used tandem mass tag labeling, TiO2 enrichment, and nanoscale liquid chromatography coupled with tandem mass spectrometry (nanol LC-MS/MS) to compare proteomic and phosphoproteomic profiling analyses of muscle between fat-1 transgenic cows and wild-type cows. A total of 1555 proteins and 900 phosphorylation sites in 159 phosphoproteins were identified in the profiling assessments, but only four differentially expressed proteins and nine differentially expressed phosphopeptides were detected in fat-1 transgenic cows relative to wild-type cows...
July 14, 2017: Transgenic Research
https://www.readbyqxmd.com/read/28709136/tetracyclines-as-a-potential-antiviral-therapy-against-crimean-congo-hemorrhagic-fever-virus-docking-and-molecular-dynamic-studies
#5
Amirhossein Sharifi, Arash Amanlou, Faezeh Moosavi-Movahedi, Sahand Golestanian, Massoud Amanlou
Crimean-Congo Hemorrhagic Fever Virus (CCHFV) is one of the deadliest human diseases with mortality rate near 50%. Special attention should be paid to this virus since there is no approved treatment for it. On the other hand, the recent outbreak of Ebola virus which is a member of hemorrhagic fever viruses shows this group of viruses can be extremely dangerous. Previous studies have indicated that nucleoprotein of CCHFV, a pivotal protein in virus replication, is an appropriate target for antiviral drug development...
July 1, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28706297/harmless-effects-of-argon-plasma-on-caudal-fin-regeneration-and-embryogenesis-of-zebrafish-novel-biological-approaches-for-safe-medical-applications-of-bioplasma
#6
Min-Kyung Nam, Goo-Young Kim, Si-Eun Yun, Ja-Young Jang, Yong-Hee Kim, Eun Ha Choi, Seongman Kang, Hyangshuk Rhim
The argon plasma jet (Ar-PJ) is widely used in medical fields such as dermatology and dentistry, and it is considered a promising tool for cancer therapy. However, the in vivo effects of Ar-PJ for medical uses have not yet been investigated, and there are no biological tools to determine the appropriate clinical dosages of Ar-PJ. In this study, we used the caudal fin and embryo of zebrafish as novel in vivo tools to evaluate the biosafety of Ar-PJ. Typically, Ar-PJ is known to induce cell death in two-dimensional (2D) cell culture systems...
July 14, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28705154/phenotypic-and-genomic-comparison-of-mycobacterium-aurum-and-surrogate-model-species-to-mycobacterium-tuberculosis-implications-for-drug-discovery
#7
Amine Namouchi, Mena Cimino, Sandrine Favre-Rochex, Patricia Charles, Brigitte Gicquel
BACKGROUND: Tuberculosis (TB) is caused by Mycobacterium tuberculosis and represents one of the major challenges facing drug discovery initiatives worldwide. The considerable rise in bacterial drug resistance in recent years has led to the need of new drugs and drug regimens. Model systems are regularly used to speed-up the drug discovery process and circumvent biosafety issues associated with manipulating M. tuberculosis. These include the use of strains such as Mycobacterium smegmatis and Mycobacterium marinum that can be handled in biosafety level 2 facilities, making high-throughput screening feasible...
July 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28684913/preparation-and-antioxidant-properties-of-selenium-nanoparticles-loaded-chitosan-microspheres
#8
Kaikai Bai, Bihong Hong, Jianlin He, Zhuan Hong, Ran Tan
Selenium nanoparticles (SeNPs), as a special form of selenium (Se) supplement, have attracted worldwide attention due to their favorable properties and unique bioactivities. Herein, an eco-friendly and economic way to prepare stable SeNPs is introduced. SeNPs were synthesized in aqueous chitosan (CTS) and then embedded into CTS microspheres by spray-drying, forming selenium nanoparticles-loaded chitosan microspheres (SeNPs-M). The physicochemical properties including morphology, elemental state, size distribution and surface potential were investigated...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28674944/a-functional-genomics-approach-to-henipavirus-research-the-role-of-nuclear-proteins-micrornas-and-immune-regulators-in-infection-and-disease
#9
Cameron R Stewart, Celine Deffrasnes, Chwan Hong Foo, Andrew G D Bean, Lin-Fa Wang
Hendra and Nipah viruses (family Paramyxoviridae, genus Henipavirus) are zoonotic RNA viruses that cause lethal disease in humans and are designated as Biosafety Level 4 (BSL4) agents. Moreover, henipaviruses belong to the same group of viruses that cause disease more commonly in humans such as measles, mumps and respiratory syncytial virus. Due to the relatively recent emergence of the henipaviruses and the practical constraints of performing functional genomics studies at high levels of containment, our understanding of the henipavirus infection cycle is incomplete...
July 4, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28668584/the-biotechnology-and-bioeconomy-landscape-in-malaysia
#10
REVIEW
Mahaletchumy Arujanan, Muthu Singaram
Since 1990s Malaysia aspired to make biotechnology and bioeconomy as her engines of economic growth to utlise the abundance of natural resources and biodiversity. The public sector plays an integral role in developing the sector and various incentives are in place for the private sector to be actively involved and to forge collaboration with the public sector. The country launched its National Biotechnology Policy in 2005 and later launched its National Bioeconomy Programme in 2010 to become the first country in South East Asia and second in Asia after China to have such an initiative...
June 28, 2017: New Biotechnology
https://www.readbyqxmd.com/read/28662750/viral-vector-biosafety-in-laboratory-animal-research
#11
Dalis E Collins, Jon D Reuter, Howard G Rush, Jason S Villano
Viral vector research presents unique occupational health and safety challenges to institutions due to the rapid development of both in vivo and in vitro gene-editing technologies. Risks to human and animal health make it incumbent on institutions to appropriately evaluate viral vector usage in research on the basis of available information and governmental regulations and guidelines. Here we review the factors related to risk assessment regarding viral vector usage in animals and the relevant regulatory documents associated with this research, and we highlight the most commonly used viral vectors in research today...
June 1, 2017: Comparative Medicine
https://www.readbyqxmd.com/read/28661192/shedding-light-on-biosafety-of-platelet-rich-plasma
#12
S Padilla, G Orive, E Anitua
No abstract text is available yet for this article.
July 6, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28657725/evaluation-of-phi6-persistence-and-suitability-as-an-enveloped-virus-surrogate
#13
Nathalia Aquino de Carvalho, Elyse N Stachler, Nicole Cimabue, Kyle Bibby
Recent outbreaks involving enveloped viruses, such as Ebola virus, have raised questions regarding the persistence of enveloped viruses in the water environment. Efforts have been made to find enveloped virus surrogates due to challenges investigating viruses that require biosafety-level 3 or 4 handling. In this study, the enveloped bacteriophage Phi6 was evaluated as a surrogate for enveloped waterborne viruses. The persistence of Phi6 was tested in aqueous conditions chosen based on previously published viral persistence studies...
July 13, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28656751/gadolinium-labeled-biodegradable-dendron-hyaluronic-acid-hybrid-and-its-subsequent-application-as-a-safe-and-efficient-magnetic-resonance-imaging-contrast-agent
#14
Chunhua Guo, Ling Sun, Hao Cai, Zhenyu Duan, Shiyong Zhang, Qiyong Gong, Kui Luo, Zhongwei Gu
Novel magnetic resonance imaging (MRI) contrast agents with high sensitivity and good biocompatibility are required for the diagnosis of cancer. Herein, we prepared and characterized the gadolinium [Gd(III)]-labeled peptide dendron-hyaluronic acid (HA) conjugate-based hybrid (dendronized-HA-DOTA-Gd) by combining the advantages of HA and the peptide dendron. The dendronized-HA-DOTA-Gd hybrid with 3.8% Gd(III) as weight percentage showed a negative zeta potential (-35 mV). The in vitro degradation results indicated that the dendronized-HA-DOTA-Gd hybrid degraded into products with low molecular weights in the presence of hyaluronidase...
July 19, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28649247/an-antigen-presenting-and-apoptosis-inducing-polymer-microparticle-prolongs-alloskin-graft-survival-by-selectively-and-markedly-depleting-alloreactive-cd8-t-cells
#15
Wei Wang, Khawar Ali Shahzad, Miaochen Li, Aifeng Zhang, Lei Zhang, Tao Xu, Xin Wan, Chuanlai Shen
Selectively depleting the pathogenic T cells is a fundamental strategy for the treatment of allograft rejection and autoimmune disease since it retains the overall immune function of host. The concept of killer artificial antigen-presenting cells (KaAPCs) has been developed by co-coupling peptide-major histocompatibility complex (pMHC) multimer and anti-Fas monoclonal antibody (mAb) onto the polymeric microparticles (MPs) to induce the apoptosis of antigen-specific T cells. But little information is available about its in vivo therapeutic potential and mechanism...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28636447/emerging-viral-infections-in-pakistan-issues-concerns-and-future-prospects
#16
Ali Talha Khalil, Muhammad Ali, Faouzia Tanveer, Muhammad Ovais, Muhammad Idrees, Zabta Khan Shinwari, James E Hollenbeck
Emerging infectious diseases pose a serious threat to public health security; this is especially true in the underdeveloped world because of limited resources to combat them. These emerging pathogens are characterized by a novel mode of pathogenesis and, in some cases, a broad host range. Over the past few decades, Pakistan has suffered a great deal from infectious diseases such as dengue, Crimean-Congo fever, hepatitis, measles, and polio. Changing climate conditions, environmental degradation, global warming, loss of biodiversity, and other ecological determinants have a direct effect on these diseases and result in the emergence and reemergence of infectious entities...
May 2017: Health Security
https://www.readbyqxmd.com/read/28628661/elimination-of-persistent-vaccine-bacteria-of-salmonella-enterica-serovar-typhimurium-in-the-guts-of-immunized-mice-by-inducible-expression-of-truncated-ynce
#17
Yiran Wang, Jianhua Li, Kun Xiong, Zhijin Chen, Chunping Zheng, Yong Tan, Yanguang Cong
Orally administered vaccine bacteria usually persist for a period of time in the intestinal tracts of immunized individuals, and are excreted in feces to the environment resulting in a potential biosafety issue. The releasing risk can be minimized by immediate elimination of the persistent vaccine bacteria once adequate protective immune responses have been elicited by the vaccine bacteria. In a previous study, inducible expression of truncated yncE gene (yncE*) was found lethal to host bacteria. This feature has an application potential in biosafety control...
2017: PloS One
https://www.readbyqxmd.com/read/28628619/south-african-ebola-diagnostic-response-in-sierra-leone-a-modular-high-biosafety-field-laboratory
#18
Janusz T Paweska, Petrus Jansen van Vuren, Gunther H Meier, Chantel le Roux, Ousman S Conteh, Alan Kemp, Cardia Fourie, Prabha Naidoo, Serisha Naicker, Phumza Ohaebosim, Nadia Storm, Orienka Hellferscee, Lisa K Ming Sun, Busisiwe Mogodi, Nishi Prabdial-Sing, Desiree du Plessis, Deidre Greyling, Shayne Loubser, Mark Goosen, Stewart D McCulloch, Terence P Scott, Alexandra Moerdyk, Wesley Dlamini, Kelfala Konneh, Idrissa L Kamara, Dauda Sowa, Samuel Sorie, Brima Kargbo, Shabir A Madhi
BACKGROUND: In August 2014, the National Institute for Communicable Diseases (NICD) in South Africa established a modular high-biosafety field Ebola diagnostic laboratory (SA FEDL) near Freetown, Sierra Leone in response to the rapidly increasing number of Ebola virus disease (EVD) cases. METHODS AND FINDINGS: The SA FEDL operated in the Western Area of Sierra Leone, which remained a "hotspot" of the EVD epidemic for months. The FEDL was the only diagnostic capacity available to respond to the overwhelming demand for rapid EVD laboratory diagnosis for several weeks in the initial stages of the EVD crisis in the capital of Sierra Leone...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28620600/biosafety-and-biosecurity-in-european-containment-level-3-laboratories-focus-on-french-recent-progress-and-essential-requirements
#19
REVIEW
Boris Pastorino, Xavier de Lamballerie, Rémi Charrel
Even if European Union (EU) Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work, national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a relatively new concept and a few EU Member States are known to have introduced national laboratory biosecurity legislation...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28617420/mirna-mediated-post-transcriptional-silencing-of-transgenes-leads-to-increased-adeno-associated-viral-vector-yield-and-targeting-specificity
#20
C A Reid, S L Boye, W W Hauswirth, D M Lipinski
The production of high titer recombinant adeno-associated virus (rAAV) vector is essential for treatment of genetic diseases affecting the retina and choroid, where anatomical constraints may limit injectable volumes. Problematically, cytotoxicity arising from over-expression of the transgene during vector production frequently leads to a reduction in vector yield. Herein, we evaluate the use of micro-RNA (miRNA) mediated silencing to limit over-expression of cytotoxic transgenes during packaging as a method of increasing vector yield...
June 15, 2017: Gene Therapy
keyword
keyword
14467
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"