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https://www.readbyqxmd.com/read/29447015/optogenetics-in-cancer-drug-discovery
#1
Michał Kiełbus, Jakub Czapiński, Adrian Odrzywolski, Grażyna Stasiak, Kamila Szymańska, Joanna Kałafut, Michał Kos, Krzysztof Giannopoulos, Andrzej Stepulak, Adolfo Rivero-Müller
The discovery and domestication of biomolecules that respond to light has taken a light of its own, providing new molecular tools with incredible spatio-temporal resolution to manipulate cellular behavior. Areas covered: The authors herein analyze the current optogenetic tools in light of their current, and potential, uses in cancer drug discovery, biosafety and cancer biology. Expert opinion: The pipeline from drug discovery to the clinic is plagued with drawbacks, where most drugs fail in either efficacy or safety...
February 15, 2018: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/29445138/non-toxic-engineered-carbon-nanodiamond-concentrations-induce-oxidative-nitrosative-stress-imbalance-of-energy-metabolism-and-mitochondrial-dysfunction-in-microglial-and-alveolar-basal-epithelial-cells
#2
Claudia G Fresta, Aishik Chakraborty, Manjula B Wijesinghe, Angela M Amorini, Giacomo Lazzarino, Giuseppe Lazzarino, Barbara Tavazzi, Susan M Lunte, Filippo Caraci, Prajnaparamita Dhar, Giuseppe Caruso
Engineered nanoparticles are finding a wide spectrum of biomedical applications, including drug delivery and capacity to trigger cytotoxic phenomena, potentially useful against tumor cells. The full understanding of their biosafety and interactions with cell processes is mandatory. Using microglial (BV-2) and alveolar basal epithelial (A549) cells, in this study we determined the effects of engineered carbon nanodiamonds (ECNs) on cell viability, nitric oxide (NO) and reactive oxygen species (ROS) production, as well as on energy metabolism...
February 14, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29436217/a-stealth-and-fully-laden-drug-carrier-self-assembled-nanogel-encapsulated-with-epigallocatechin-gallate-and-sirna-for-drug-resistant-breast-cancer-therapy
#3
Jie Ding, Tingxizi Liang, Qianhao Min, Li-Ping Jiang, Jun-Jie Zhu
For co-delivery of therapeutic genes and chemical agents in combined therapy, the ideal drug delivery system (DDS) entails high-capacity and low-body toxicity carriers, allowing adequate drug dose for tumor region while yielding low residue in normal tissue. To augment the gene/drug load capacity and circumvent the potential toxicity brought by traditional inorganic and polymeric nanocarriers, a "stealth" carrier was herein designed in a simple self-assembly of (-)-epigallocatechin-3-O-gallate (EGCG) and small interfering RNA (siRNA) by recruiting protamine as biodegradable medium for the treatment of drug-resistant triple-negative breast cancer (TNBC)...
February 13, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29434059/certification-validation-of-biosafety-level-2-biosafety-level-3-laboratories-in-indian-settings-common-issues
#4
Devendra T Mourya, Pragya D Yadav, Ajay Khare, Anwar H Khan
With increasing awareness regarding biorisk management worldwide, many biosafety laboratories are being setup in India. It is important for the facility users, project managers and the executing agencies to understand the process of validation and certification of such biosafety laboratories. There are some international guidelines available, but there are no national guidelines or reference standards available in India on certification and validation of biosafety laboratories. There is no accredited government/private agency available in India to undertake validation and certification of biosafety laboratories...
October 2017: Indian Journal of Medical Research
https://www.readbyqxmd.com/read/29422865/dietary-safety-assessment-of-flk1-transgenic-fish
#5
Yalan Wei, Ling Huang, Jinghui Cao, Chenghui Wang, Jizhou Yan
Genetic engineering, also called genetic modification, is facing with growing demands of aquaculture and aquatic products. Although various genetically modified (GM) aquatics have been generated, it is important to evaluate biosafety of GM organisms on the human health before entering into our food chain. For this purpose, we establish a zebrafish wild adult feeding Flk1-transgenic larvae model to examine the predatory fish's histology in multiple tissues, and the global gene expression profile in the liver...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29422098/screening-of-intact-yeasts-and-cell-extracts-to-reduce-scrapie-prions-during-biotransformation-of-food-waste
#6
David Huyben, Sofia Boqvist, Volkmar Passoth, Lena Renström, Ulrika Allard Bengtsson, Olivier Andréoletti, Anders Kiessling, Torbjörn Lundh, Ivar Vågsholm
Yeasts can be used to convert organic food wastes to protein-rich animal feed in order to recapture nutrients. However, the reuse of animal-derived waste poses a risk for the transmission of infectious prions that can cause neurodegeneration and fatality in humans and animals. The aim of this study was to investigate the ability of yeasts to reduce prion activity during the biotransformation of waste substrates-thereby becoming a biosafety hurdle in such a circular food system. During pre-screening, 30 yeast isolates were spiked with Classical Scrapie prions and incubated for 72 h in casein substrate, as a waste substitute...
February 8, 2018: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/29421551/suppress-orthotopic-colon-cancer-and-its-metastasis-through-exact-targeting-and-highly-selective-drug-release-by-a-smart-nanomicelle
#7
Chunqi Zhu, Hanbo Zhang, Wei Li, Lihua Luo, Xiaomeng Guo, Zuhua Wang, Fenfen Kong, Qingpo Li, Jie Yang, Yongzhong Du, Jian You
The treatment of metastatic cancer is a huge challenge at the moment. Highly precise targeting delivery and drug release in tumor have always been our pursuit in cancer therapy, especially to advance cancer with metastasis, for increasing the efficacy and biosafety. We established a smart nanosized micelle, formed by tocopherol succinate (TOS) conjugated hyaluronic acid (HA) using a disulfide bond linker. The micelle (HA-SS-TOS, HSST) can highly specifically bind with CD44 receptor over-expressed tumor, and response selectively to high GSH level in the cells, inducing disulfide bond breakage and the release of the payload (paclitaxel, PTX)...
February 1, 2018: Biomaterials
https://www.readbyqxmd.com/read/29417054/aujeszky-s-disease-and-hepatitis-e-viruses-transmission-between-domestic-pigs-and-wild-boars-in-corsica-evaluating-the-importance-of-wild-domestic-interactions-and-the-efficacy-of-management-measures
#8
François Charrier, Sophie Rossi, Ferran Jori, Oscar Maestrini, Céline Richomme, François Casabianca, Christian Ducrot, Johan Jouve, Nicole Pavio, Marie-Frédérique Le Potier
Wildlife species as reservoirs of infectious pathogens represent a serious constraint in the implementation of disease management strategies. In the Mediterranean island of Corsica, the dynamics of hepatitis E virus (HEV) and Aujeszky's disease virus (ADV) are suspected to be influenced by interactions between wild and domestic pigs. To improve our understanding of these influences, we first compared the seroprevalences of both viruses in domestic pig populations from different locations with contrasted levels of wild-domestic interactions, ADV vaccination, biosafety, and farm husbandry...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29415767/swinepox-virus-vector-based-vaccines-attenuation-and-biosafety-assessments-following-subcutaneous-prick-inoculation
#9
Xiaomin Yuan, Huixing Lin, Bin Li, Kongwang He, Hongjie Fan
Swinepox virus (SPV) has several advantages as a potential clinical vector for a live vector vaccine. In this study, to obtain a safer and more efficient SPV vector, three SPV mutants, Δ003, Δ010, and ΔTK were successfully constructed. A virus replication experiment showed that these SPV mutants had lower replication abilities compared to wtSPV in 10 different host-derived cell lines. Animal experiments with mouse and rabbit models demonstrate that these three mutants and wtSPV did not cause any clinical signs of dermatitis...
February 7, 2018: Veterinary Research
https://www.readbyqxmd.com/read/29415704/a-new-approach-for-detecting-adventitious-viruses-shows-sf-rhabdovirus-negative-sf-rvn-cells-are-suitable-for-safe-biologicals-production
#10
Christoph Geisler
BACKGROUND: Adventitious viral contamination in cell substrates used for biologicals production is a major safety concern. A powerful new approach that can be used to identify adventitious viruses is a combination of bioinformatics tools with massively parallel sequencing technology. Typically, this involves mapping or BLASTN searching individual reads against viral nucleotide databases. Although extremely sensitive for known viruses, this approach can easily miss viruses that are too dissimilar to viruses in the database...
February 7, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/29406800/rapid-molecular-diagnostics-for-multi-drug-resistant-tuberculosis-in-india
#11
Rajeswari Ramachandran, M Muniyandi
Rapid molecular diagnostic methods help in the detection of TB and Rifampicin resistance. These methods detect TB early, are accurate and play a crucial role in reducing the burden of drug resistant tuberculosis. Areas covered: This review analyses rapid molecular diagnostic tools used in the diagnosis of MDR-TB in India, such as the Line Probe Assay and GeneXpert. We have discussed the burden of MDR-TB and the impact of recent diagnostic tools on case detection and treatment outcomes. This review also discusses the costs involved in establishing these new techniques in India...
February 6, 2018: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/29406119/intranasal-administration-of-tetrabromobisphenol-a-bis-2-hydroxyethyl-ether-induces-neurobehavioral-changes-in-neonatal-sprague-dawley-rats
#12
Qian S Liu, Na Liu, Zhendong Sun, Qunfang Zhou, Guibin Jiang
Tetrabromobisphenol A (TBBPA) and its derivatives are now being highly concerned due to their emerging environmental occurrence and deleterious effects on non-target organisms. Considering the potential neurotoxicity of TBBPA derivatives which has been demonstrated in vitro, what could happen in vivo is worthy of being studied. Tetrabromobisphenol A bis(2-hydroxyethyl ether) (TBBPA-BHEE), a representative TBBPA derivative, was selected for a 21-day exposure experiment on neonatal Sprague Dawley (SD) rats through intranasal administration...
January 2018: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/29405649/2d-ultrathin-mxene-based-drug-delivery-nanoplatform-for-synergistic-photothermal-ablation-and-chemotherapy-of-cancer
#13
Xiaoxia Han, Ju Huang, Han Lin, Zhigang Wang, Pan Li, Yu Chen
Two-dimensional (2D) MXenes, as a new 2D functional material nanosystem, have been extensively explored for broad applications. However, their specific performance and applications in theranostic nanomedicine have still rarely been explored. This work reports on the drug-delivery performance and synergistic therapeutic efficiency of Ti3 C2 MXenes for highly efficient tumor eradication. These Ti3 C2 MXenes not only possess high drug-loading capability of as high as 211.8%, but also exhibit both pH-responsive and near infrared laser-triggered on-demand drug release...
February 5, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29403275/development-of-biocompatible-and-vegf-targeted-paclitaxel-nanodrugs-on-albumin-and-graphene-oxide-dual-carrier-for-photothermal-triggered-drug-delivery-in-vitro-and-in-vivo
#14
Wentao Deng, Juhui Qiu, Shaoting Wang, Zhi Yuan, Yuefeng Jia, Hailin Tan, Jiru Lu, Ruqiang Zheng
In this study, we performed the characterization and synthesis of biocompatible and targeted albumin and graphene oxide (GO) dual-carrier paclitaxel (PTX) nanoparticles for photothermal-triggered tumor therapy. PTX absorbed on GO nanosheets as cores were coated with human serum albumin (HSA), following surface conjugation with monoclonal antibodies (mAb) against vascular endothelial growth factor (VEGF; denoted as mAbVEGF) via polyethylene glycol linker to form targeted nanoparticles (PTX-GHP-VEGF). The spherical nanoparticles were 191±5 nm in size with good stability and biocompatibility...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29396297/in-vitro-regeneration-of-patient-specific-ear-shaped-cartilage-and-its-first-clinical-application-for-auricular-reconstruction
#15
Guangdong Zhou, Haiyue Jiang, Zongqi Yin, Yu Liu, Qingguo Zhang, Chen Zhang, Bo Pan, Jiayu Zhou, Xu Zhou, Hengyun Sun, Dan Li, Aijuan He, Zhiyong Zhang, Wenjie Zhang, Wei Liu, Yilin Cao
Microtia is a congenital external ear malformation that can seriously influence the psychological and physiological well-being of affected children. The successful regeneration of human ear-shaped cartilage using a tissue engineering approach in a nude mouse represents a promising approach for auricular reconstruction. However, owing to technical issues in cell source, shape control, mechanical strength, biosafety, and long-term stability of the regenerated cartilage, human tissue engineered ear-shaped cartilage is yet to be applied clinically...
January 13, 2018: EBioMedicine
https://www.readbyqxmd.com/read/29374599/ph-triggered-charge-reversible-of-glycol-chitosan-conjugated-carboxyl-graphene-for-enhancing-photothermal-ablation-of-focal-infection
#16
Wei Qian, Chang Yan, Danfeng He, Yunzhou Yu, Long Yuan, Menglong Liu, Gaoxing Luo, Jun Deng
Subcutaneous abscesses infected by multidrug-resistant bacteria are becoming an increasing challenge to human health. To address this challenge, a surface-adaptive and biocompatible glycol chitosan conjugated carboxyl graphene (GCS-CG) is developed, which exhibits unique self-adaptive target to the acidic microenvironment of abscess (∼ pH 6.3) and no damage to the healthy tissue (pH 7.4) around the abscess. Originally, following conjugated with GCS, the absorbance of CG obviously increased in the near-infrared (NIR) region, enabling GCS-CG to generate an increment amount of heat...
January 24, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29364521/biosafety-in-handling-gene-transfer-vectors
#17
Scott Swindle
This unit is devoted to safety issues that must be considered when generating and working with the most common vectors under development for human gene therapy today. © 2018 by John Wiley & Sons, Inc.
January 24, 2018: Current Protocols in Human Genetics
https://www.readbyqxmd.com/read/29361752/crispr-cas9-advancing-orthopoxvirus-genome-editing-for-vaccine-and-vector-development
#18
REVIEW
Arinze Okoli, Malachy I Okeke, Morten Tryland, Ugo Moens
The clustered regularly interspaced short palindromic repeat (CRISPR)/associated protein 9 (Cas9) technology is revolutionizing genome editing approaches. Its high efficiency, specificity, versatility, flexibility, simplicity and low cost have made the CRISPR/Cas9 system preferable to other guided site-specific nuclease-based systems such as TALENs (Transcription Activator-like Effector Nucleases) and ZFNs (Zinc Finger Nucleases) in genome editing of viruses. CRISPR/Cas9 is presently being applied in constructing viral mutants, preventing virus infections, eradicating proviral DNA, and inhibiting viral replication in infected cells...
January 22, 2018: Viruses
https://www.readbyqxmd.com/read/29359993/the-economic-and-environmental-cost-of-delayed-gm-crop-adoption-the-case-of-australia-s-gm-canola-moratorium
#19
Scott Biden, Stuart J Smyth, David Hudson
Incorporating socio-economic considerations (SECs) into national biosafety regulations regarding genetically modified (GM) crops have opportunity costs. Australia approved the cultivation of GM canola through a science-based risk assessment in 2003, but allowed state moratoria to be instituted based on potential trade impacts over the period 2004 to 2008 and 2010 in the main canola growing states. This analysis constructs a counterfactual assessment using Canadian GM canola adoption data to create an S-Curve of adoption in Australia to measure the environmental and economic opportunity costs of Australia's SEC-based moratoria between 2004 and 2014...
January 23, 2018: GM Crops & Food
https://www.readbyqxmd.com/read/29350584/developing-standard-operating-procedures-for-gene-drive-research-in-disease-vector-mosquitoes
#20
Zach N Adelman, David Pledger, Kevin M Myles
The biosafety landscape for studies involving gene drive in arthropods is at a critical juncture. Numerous arthropod species represent potential targets for gene-drive-based population suppression or replacement, due to the large number of arthropods that transmit diseases, damage crops, or act as deleterious invasive species. Containment measures for gene drive research in arthropods have been discussed in the literature, but the importance of developing safe and effective standard operating procedures (SOPs) for these types of experiments has not been adequately addressed...
January 19, 2018: Pathogens and Global Health
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