Tomas Kanholm, Uzma Rentia, Melissa Hadley, Jennifer A Karlow, Olivia L Cox, Noor Diab, Matthew L Bendall, Tyson Dawson, James I McDonald, Wenbing Xie, Keith A Crandall, Kathleen H Burns, Stephen B Baylin, Hariharan Easwaran, Katherine B Chiappinelli
Transposable elements (TEs) are typically silenced by DNA methylation and repressive histone modifications in differentiated healthy human tissues. However, TE expression increases in a wide range of cancers and is correlated with global hypomethylation of cancer genomes. We assessed expression and DNA methylation of TEs in fibroblast cells that were serially transduced with hTERT, SV40, and HRASR24C to immortalize and then transform them, which models the different steps of the tumorigenesis process. RNA-sequencing and whole-genome bisulfite sequencing were performed at each stage of transformation...
May 30, 2023: Cancer Research