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Jean-François Boisclair Lachance, Jemma L Webber, Lu Hong, Aaron Dinner, Ilaria Rebay
Cis -regulatory modules (CRMs) are defined by unique combinations of transcription factor-binding sites. Emerging evidence suggests that the number, affinity, and organization of sites play important roles in regulating enhancer output and, ultimately, gene expression. Here, we investigate how the cis -regulatory logic of a tissue-specific CRM responsible for even-skipped ( eve ) induction during cardiogenesis organizes the competing inputs of two E-twenty-six (ETS) members: the activator Pointed (Pnt) and the repressor Yan...
March 13, 2018: Genes & Development
John M Hickey, Vishal M Toprani, Kawaljit Kaur, Ravi P N Mishra, Akshay Goel, Natalia Oganesyan, Andrew Lees, Robert Sitrin, Sangeeta B Joshi, David B Volkin
CRM197 , a single amino acid mutant of diphtheria toxoid, is a commonly used carrier protein in commercial polysaccharide-protein conjugate vaccines. In this study, CRM197 proteins from three different expression systems and five different manufacturers were obtained for an analytical comparability assessment using a wide variety of physicochemical and in vitro antigenic binding assays. A comprehensive analysis of the five CRM197 molecules demonstrate that recombinant CRM197 's expressed in heterologous systems (E...
March 8, 2018: Journal of Pharmaceutical Sciences
Fabrizio Bracco, Gabriele de Tonetti, Michele Masini, Marcello Passarelli, Francesca Geretto, Danilo Celleno
Human factors are the most relevant issues contributing to adverse events in obstetrics. Specific training of Crisis Resource Management (CRM) skills (i.e., problem solving and team management, resource allocation, awareness of environment, and dynamic decision-making) is now widespread and is often based on High Fidelity Simulation. In order to be used as a guideline in simulated scenarios, CRM skills need to be mapped to specific and observable behavioral markers. For this purpose, we developed a set of observable behaviors related to the main elements of CRM in the delivery room...
March 3, 2018: International Journal of Environmental Research and Public Health
Claudia R Gerstle
Aviation and healthcare are complex industries and share many similarities: the cockpit and the operating theater, the captain and the surgeon. While North American commercial aviation currently enjoys a tremendous safety record, it was not always this way. A spike of accidents in 1973 caused 3214 aviation-related fatalities. Over the past 20years, the rate of fatal accidents per million flights fell by a factor of five, while air traffic increased by more than 86%. There have been no fatalities on a U.S. carrier for over 12years...
February 8, 2018: Journal of Pediatric Surgery
Nomvano Mketo, Philiswa N Nomngongo, J Catherine Ngila
A rapid three-step sequential extraction method was developed under microwave radiation followed by inductively coupled plasma-optical emission spectroscopic (ICP-OES) and ion-chromatographic (IC) analysis for the determination of sulphur forms in coal samples. The experimental conditions of the proposed microwave-assisted sequential extraction (MW-ASE) procedure were optimized by using multivariate mathematical tools. Pareto charts generated from 23 full factorial design showed that, extraction time has insignificant effect on the extraction of sulphur species, therefore, all the sequential extraction steps were performed for 5 min...
May 15, 2018: Talanta
Yasmin Thabet, Dominique Lunter, Joerg Breitkreutz
Various drug therapies require more than one active pharmaceutical ingredient (API) for an effective treatment. There are many advantages, e.g. to improve the compliance or pharmacodynamic response in comparison to a monotherapy or to increase the therapy safety. Until now, there are only a few products available for the paediatric population due to the lack of age appropriate dosage forms or studies proving the efficacy and safety of these products. This study aims to develop orodispersible films (ODFs) in a continuous solvent casting process as child appropriate dosage form containing both enalapril maleate (EM) and hydrochlorothiazide (HCT) separated in different film layers...
February 27, 2018: European Journal of Pharmaceutical Sciences
Laura Freije-Carrelo, Laura Alonso Sobrado, Mariella Moldovan, Jorge Ruiz Encinar, Jose Ignacio Garcia-Alonso
Dissolved inorganic carbon (DIC) is one of the most important parameters to be measured in seawaters for climate change studies. Its quantitative assessment requires analytical methodologies with overall uncertainties around 0.05% RSD for clear evaluation of temporal trends. Herein, two alternative Isotope Dilution Mass Spectrometry (IDMS) methodologies (on-line and species-specific) using an Isotope Ratio Mass Spectrometer (IRMS) and two calculation procedures for each methodology have been compared. As a result, a new method for the determination of DIC in seawaters, based on species-specific IDMS with Isotope Pattern Deconvolution calculation, was developed and validated...
March 2, 2018: Analytical Chemistry
Francesco Pichi, K Bailey Freund, Antonio Ciardella, Mariachiara Morara, Emad B Abboud, Nicola Ghazi, Christine Dackiw, Netan Choudhry, Eduardo Cunha Souza, Leonardo Provetti Cunha, J Fernando Arevalo, T Y Alvin Liu, Adam Wenick, Lingmin He, Guadalupe Villarreal, Piergiorgio Neri, David Sarraf
Importance: Congenital retinal macrovessel (CRM) is a rarely reported venous malformation of the retina that is associated with venous anomalies of the brain. Objective: To study the multimodal imaging findings of a series of eyes with congenital retinal macrovessel and describe the systemic associations. Design, Setting, and Participants: In this cross-sectional multicenter study, medical records were retrospectively reviewed from 7 different retina clinics worldwide over a 10-year period (2007-2017)...
March 1, 2018: JAMA Ophthalmology
Federico Pietrocola, Francesca Castoldi, Maria Markaki, Sylvie Lachkar, Guo Chen, David P Enot, Sylvere Durand, Noelie Bossut, Mingming Tong, Shoaib A Malik, Friedemann Loos, Nicolas Dupont, Guillermo Mariño, Nejma Abdelkader, Frank Madeo, Maria Chiara Maiuri, Romano Kroemer, Patrice Codogno, Junichi Sadoshima, Nektarios Tavernarakis, Guido Kroemer
The age-associated deterioration in cellular and organismal functions associates with dysregulation of nutrient-sensing pathways and disabled autophagy. The reactivation of autophagic flux may prevent or ameliorate age-related metabolic dysfunctions. Non-toxic compounds endowed with the capacity to reduce the overall levels of protein acetylation and to induce autophagy have been categorized as caloric restriction mimetics (CRMs). Here, we show that aspirin or its active metabolite salicylate induce autophagy by virtue of their capacity to inhibit the acetyltransferase activity of EP300...
February 27, 2018: Cell Reports
Marine Lacomme, François Medevielle, Henri-Marc Bourbon, Elodie Thierion, Dirk-Jan Kleinjan, Mélanie Roussat, Fabienne Pituello, Sophie Bel-Vialar
Proper embryonic development relies on a tight control of spatial and temporal gene expression profiles in a highly regulated manner. One good example is the ON/OFF switching of the transcription factor PAX6 that governs important steps of neurogenesis. In the neural tube PAX6 expression is initiated in neural progenitors through the positive action of retinoic acid signaling and downregulated in neuronal precursors by the bHLH transcription factor NEUROG2. How these two regulatory inputs are integrated at the molecular level to properly fine tune temporal PAX6 expression is not known...
February 24, 2018: Developmental Biology
Tomoko Ariga, Yanbei Zhu, Mika Ito, Toshiko Takatsuka, Shinya Terauchi, Akira Kurokawa, Kazumi Inagaki
Area densities of Au/Ni/Cu layers on a Cr-coated quartz substrate were characterized to certify a multiple-metal-layer certified reference material (NMIJ CRM5208-a) that is intended for use in the analysis of the layer area density and the thickness by an X-ray fluorescence spectrometer. The area densities of Au/Ni/Cu layers were calculated from layer mass amounts and area. The layer mass amounts were determined by using wet chemical analyses, namely inductively coupled plasma mass spectrometry (ICP-MS), isotope-dilution (ID-) ICP-MS, and inductively coupled plasma optical emission spectrometry (ICP-OES) after dissolving the layers with diluted mixture of HCl and HNO3 (1:1, v/v)...
February 27, 2018: Analytical and Bioanalytical Chemistry
Khaled Abouelezz, Jianfeng Yuan, Guiping Wang, Guozhi Bian
The cassava root meal (CRM) has been utilized as a cheap energy alternative to replace maize in poultry diets. Recently, the CRM in turn has an increasing demand for starch extraction industry, which renders large amounts of residues. This study evaluated the nutrient composition, amino acid profile, and feeding value of cassava starch extraction residue meal (CReM) for growing ducks. A total of 960, 11-day-old, ducklings were housed in 24 floor pens and allocated randomly into four dietary treatments: (i) 0CReM (control), (ii) 50 g CReM/kg, (iii) 100 g CReM/kg, and (iv) 150 g CReM/kg...
February 26, 2018: Tropical Animal Health and Production
Mesay Mulugeta Wolle, Sean D Conklin
Single laboratory validation of a method for arsenic speciation analysis in seafood and seaweed is presented. The method is based on stepwise extraction of water-soluble and non-polar arsenic with hot water and a mixture of dichloromethane and methanol, respectively. While the water-soluble arsenicals were speciated by anion and cation exchange liquid chromatography inductively coupled plasma mass spectrometry (LC-ICP-MS), the non-polar arsenicals were collectively determined by ICP-MS after digestion in acid...
February 23, 2018: Analytical and Bioanalytical Chemistry
Hong-Peng Jiang, Yan-Sen Li, Bo Wang, Chang Wang, Fan Liu, Zhan-Long Shen, Ying-Jiang Ye, Shan Wang
BACKGROUND: Since 2010, comparative studies on transanal and laparoscopic total mesorectal excision (TME) have been published and it remains unclear about the oncological benefit from transanal total mesorectal excision (taTME). METHODS: We have searched English databases to identify all taTME studies published between January 2010 and August 2017. Pathological outcomes included circumferential resection margin (CRM), positive CRM (< 1 M), length of distal resection margin (DRM), positive DRM, quality of mesorectum (complete mesorectum), harvested lymph node, and length of the specimen...
February 20, 2018: Surgical Endoscopy
Anna Baranik, Rafal Sitko, Anna Gagor, Ignasi Queralt, Eva Margui, Beata Zawisza
Graphene oxide decorated with cerium (IV) oxide (GO/CeO2) was synthesized and applied in adsorption of several metal ions such as As(III), As(V), Se(IV), Cu(II) and Pb(II) from aqueous samples. The important feature of GO/CeO2 nanocomposite is also its selectivity towards selenite in the presence of selenate. The structure of GO/CeO2 has been proven by microscopic and spectroscopic techniques. The maximum adsorption capacities of GO/CeO2 calculated by Langmuir model towards arsenic, selenium, copper and lead ions are between 6 and 30 mg g-1...
February 18, 2018: Analytical Chemistry
Anu Carpelan, J Karvonen, P Varpe, A Rantala, A Kaljonen, J Grönroos, H Huhtinen
PURPOSE: To analyze the results of abdominoperineal excisions (APE) for locally advanced rectal cancer at our institution before and after the adoption of extralevator abdominoperineal excision (ELAPE) with a special reference to long-term survival. METHODS: A retrospective cohort study conducted in a tertiary referral center. All consecutive patients operated for locally advanced (TNM classification T3-4) rectal cancer with APE in 2004-2009 were compared to patients with similar tumors operated with ELAPE in 2009-2016...
February 14, 2018: International Journal of Colorectal Disease
Megan M Wenner, Kumba Adia Hinds, Jeffrey T Howard, Corinne D Nawn, Nina S Stachenfeld, Victor A Convertino
BACKGROUND: The measurement of Compensatory Reserve (CRM) has been established to accurately measure the body's total integrated capacity to compensate for physiological states of reduced central blood volume, and predict hemodynamic decompensation associated with inadequate tissue oxygenation. We previously demonstrated that African American (AA) women have a higher tolerance to reductions in central blood volume. Therefore, we tested the hypothesis that the CRM would identify racial differences during simulated hemorrhage, prior to the onset of traditional signs/symptoms...
February 14, 2018: Journal of Trauma and Acute Care Surgery
Benjamin D Pollock, Emily W Harville, Katherine T Mills, Wan Tang, Wei Chen, Lydia A Bazzano
BACKGROUND: Economic literature shows that a child's future earnings are predictably influenced by parental income, providing an index of "socioeconomic mobility," or the ability of a person to move towards a higher socioeconomic status from childhood to adulthood. We adapted this economic paradigm to examine cardiovascular risk mobility (CRM), or whether there is life course mobility in relative cardiovascular risk. METHODS AND RESULTS: Participants from the BHS (Bogalusa Heart Study) with 1 childhood and 1 adult visit from 1973 to 2016 (n=7624) were considered...
February 6, 2018: Journal of the American Heart Association
Ziwei Zhang, Dominique Jasmin Lunter
The purpose of this study was to investigate the impact of emulsifiers and formulations on intercellular lipids of porcine stratum corneum (SC) and evaluate confocal Raman microscopy (CRM) as an alternative method in this research context. To this end, four different formulations were used: three conventional creams that contained ionic and/or non-ionic emulsifiers and one surfactants-free emulsion stabilized by a polymeric emulsifier. Additionally, all emulsifiers were tested in aqueous solution/dispersion in the respective concentrations as present in the formulations...
February 8, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Simon Olonkwoh Salihu, Nor Kartini Abu Bakar
In this study, a simple sample preparation method was developed for the determination of tri-and hexavalent chromium in water samples. It utilizes a pre-heated customized glass tube (CGT), to supply the heat energy required for the reaction of Cr(III) with ammonium pyrrolidinedithiocarbamate (APDC). The products of the Cr complexes, tris(1-pyrrolidinecarbodithioato)chromium(III) and bis(1-pyrrolidinecarbodithioato)[1-pyrrolidinecarbodithio(thioperoxoato)]chromium(III) were chromatographed with Shimadzu LC-20AT and Zobax Eclipse C18 (150mm × 4...
May 1, 2018: Talanta
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