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Intervertebral disc degeneration

Andrew J Teichtahl, Donna M Urquhart, Yuanyuan Wang, Anita E Wluka, Richard O'Sullivan, Graeme Jones, Flavia M Cicuttini
BACKGROUND: Degenerative disc disease of the lumbar spine is common, with severe disease increasing the risk for chronic low back pain. This cross-sectional study examined whether disc degeneration is representative of a 'whole-organ' pathology, by examining its association with bone (vertebral endplate) and soft tissue (paraspinal muscle fat) abnormalities. METHODS: Seventy-two community-based individuals unselected for low back pain, had Magnetic Resonance Imaging (MRI)...
October 21, 2016: BMC Musculoskeletal Disorders
Ryan J Campbell, Ralph J Mobbs, Kevin Phan
No abstract text is available yet for this article.
September 2016: J Spine Surg
Bin Yue, Yazhou Lin, Xuexiao Ma, Guoqing Zhang, Bohua Chen
The aim of the current study was to use gene therapy to attenuate or reverse the degenerative process within the intervertabral disc. The effect of survivin gene therapy via lentiviral vector transfection on the course of intervertebral disc degeneration was investigated in the current study in an in vivo rabbit model. A total of 15 skeletally mature female New Zealand White rabbits were randomly divided into three groups: Punctured blank control group (group A, n=5), punctured empty vector control group (group B, n=5) and the treatment group (group C, n=5)...
October 12, 2016: Molecular Medicine Reports
Sally Roberts, Pauline Colombier, Aneka Sowman, Claire Mennan, Jan H D Rölfing, Jérôme Guicheux, James R Edwards
The extent of ageing in the musculoskeletal system during the life course affects the quality and length of life. Loss of bone, degraded articular cartilage, and degenerate, narrowed intervertebral discs are primary features of an ageing skeleton, and together they contribute to pain and loss of mobility. This review covers the cellular constituents that make up some key components of the musculoskeletal system and summarizes discussion from the 2015 Aarhus Regenerative Orthopaedic Symposium (AROS) (Regeneration in the Ageing Population) about how each particular cell type alters within the ageing skeletal microenvironment...
October 17, 2016: Acta Orthopaedica
Feng Cai, Feng Wang, Xin Hong, Xin-Hui Xie, Rui Shi, Zhi-Yang Xie, Xiao-Tao Wu
OBJECTIVES: Activation of acid-sensing ion channel 1a (ASIC1a) is responsible for tissue injury caused by acidosis in nervous systems. But its physiological and pathological roles in nucleus pulposus cells (NPCs) are unclear. The aim of this study is to investigate whether ASIC1a regulates the survival of NPCs in the acidic environment of degenerated discs. MATERIALS AND METHODS: NPCs were isolated and cultured followed by immunofluorescent staining and Western-blot analysis for ASIC1a...
August 2016: Iranian Journal of Basic Medical Sciences
Zemin Li, Xiaolin Wang, Hehai Pan, Hao Yang, Xiang Li, Kuibo Zhang, Hua Wang, Zhaomin Zheng, Hui Liu, Jianru Wang
OBJECTIVE: This study was to investigate whether resistin induces the expression of CCL4 during IVDD and whether Toll-like receptor-4 (TLR-4) and the NF-κB signaling pathway are involved in this process. METHODS: The expression pattern of resistin and CCL4 in different degenerated human NP tissues were measured by qPCR; Effect of resistin on the migration of macrophages was measured by cell migration assay. Resistin-induced CCL4 expression were analyzed by qPCR, ELISA and cell immunofluorescence...
October 10, 2016: Osteoarthritis and Cartilage
Carla Cunha, Catarina R Almeida, Maria Inês Almeida, Andreia M Silva, Maria Molinos, Sofia Lamas, Catarina L Pereira, Graciosa Q Teixeira, António T Monteiro, Susana G Santos, Raquel M Gonçalves, Mário A Barbosa
: : Cell therapies for intervertebral disc (IVD) regeneration presently rely on transplantation of IVD cells or stem cells directly to the lesion site. Still, the harsh IVD environment, with low irrigation and high mechanical stress, challenges cell administration and survival. In this study, we addressed systemic transplantation of allogeneic bone marrow mesenchymal stem cells (MSCs) intravenously into a rat IVD lesion model, exploring tissue regeneration via cell signaling to the lesion site...
October 11, 2016: Stem Cells Translational Medicine
Geoffrey J Kerr, Matthew R McCann, Jennifer K Branch, Anusha Ratneswaran, Michael A Pest, David W Holdsworth, Frank Beier, S Jeffrey Dixon, Cheryle A Séguin
OBJECTIVE: Whole-body vibration (WBV) platforms are commercially available devices that are used clinically as potential treatments for numerous musculoskeletal conditions based on their reported ability to increase bone mineral density and muscle strength. Despite widespread use, there is an alarming lack of understanding of the direct effects of WBV on joint health. Previous work by our lab demonstrated that repeated exposure to WBV using protocols that model those used clinically, induces intervertebral disc degeneration and osteoarthritis-like damage in the knee of skeletally mature, male mice of a single outbred strain (CD-1)...
October 6, 2016: Osteoarthritis and Cartilage
Bin Wu, Chunyang Meng, Haibin Wang, Cunling Jia, Yifeng Zhao
OBJECTIVE: Post-operation of cervical decompression fusion and internal fixation (CDF) accelerated adjacent segment disc degeneration (ASD). It is not clear that whether instability of one single segmental accelerates the degeneration of adjacent segment disc. This study aims to explore the effect of cervical instability on the change of morphology and biochemistry in adjective segment (above) in the L5/6 cervical instability rabbit models. METHODS: Thirty-two mature New Zealand white rabbits (3000±250g) were randomly divided into two groups, control group (n=8) and model group (n=24)...
October 4, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Nadja A Farshad-Amacker, Alexander Hughes, Richard J Herzog, Burkhardt Seifert, Mazda Farshad
OBJECTIVES: The association of disc degeneration (DD) and vertebral endplate degeneration (EPD) is still not well understood. This study aimed to find segmental predictive risk factors for DD and EPD and to illuminate associations of the disc, endplate and bone marrow changes in the process of degeneration. METHODS: After institutional review board approval, 450 lumbar levels, followed up with MRI for at least 4 years, were retrospectively graded for DD according to Pfirrmann (PFG), for EPD according to the endplate score (EPS) and according to the presence, extension and type of Modic changes (MC)...
October 5, 2016: European Radiology
Urška Potočar, Samo Hudoklin, Mateja Erdani Kreft, Janja Završnik, Krešimir Božikov, Mirjam Fröhlich
Cell therapies present a feasible option for the treatment of degenerated cartilaginous and intervertebral disc (IVD) tissues. Microenvironments of these tissues are specific and often differ from the microenvironment of cells that, could be potentially used for therapy, e.g. human adipose-derived stem cells (hASC). To ensure safe and efficient implantation of hASC, it is important to evaluate how microenvironmental conditions at the site of implantation affect the implanted cells. This study has demonstrated that cartilaginous tissue-specific osmolarities ranging from 400-600 mOsm/L affected hASC in a dose- and time-dependent fashion in comparison to 300 mOsm/L...
2016: PloS One
Ann Ouyang, Alec E Cerchiari, Xinyan Tang, Ellen Liebenberg, Tamara Alliston, Zev J Gartner, Jeffrey C Lotz
Tissue engineering constructs to treat intervertebral disc degeneration must adapt to the hypoxic and inflammatory degenerative disc microenvironment. The objective of this study was to determine the effects of two key design factors, cell type and cell configuration, on the regenerative potential of nucleus pulposus cell (NPC) and mesenchymal stem cell (MSC) constructs. Anabolic and catabolic activity was quantified in constructs of varying cell type (NPCs, MSCs, and a 50:50 coculture) and varying configuration (individual cells and micropellets)...
October 4, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Christoph Schleich, Falk Miese, Anja Müller-Lutz, Johannes Boos, Joel Aissa, Adrian Nasca, Frank Zaucke, Tamara Bostelmann, Lars Schimmöller, Bernd Bittersohl, Hans-Jakob Steiger, Gerald Antoch, Richard Bostelmann
The study was performed to preoperatively assess the cartilage integrity of cervical intervertebral discs (IVDs) using Delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC). Therefore, 53 cervical intervertebral discs of nine preoperative patients with neck and shoulder/arm pain scheduled for discectomy (five females, four males; mean age: 47.1 ± 8.4 years; range: 36-58 years) were included for biochemical analysis in this retrospective study. The patients underwent 3T magnetic resonance imaging (MRI) including biochemical imaging with dGEMRIC and morphological, sagittal T2 weighted (T2w) imaging...
October 4, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Shady Elmasry, Shihab Asfour, Juan Pablo de Rivero Vaccari, Francesco Travascio
Insulin-like growth factor-1 (IGF-1) is well-known for upregulating cell proliferation and biosynthesis of the extracellular matrix in the intervertebral disc (IVD). Pathological conditions, such as obesity or chronic kidney disease cause IGF-1 deficiency in plasma. How this deficiency impacts disc homeostasis remains unknown. Pro-anabolic approaches for the treatment of disc degeneration based on enhancing IGF-1 bioavailability to tissue-cells are considered, but knowledge of their effectiveness in enhancing cellular anabolism of a degenerated disc is limited...
September 22, 2016: Computers in Biology and Medicine
Naimisha Beeravolu, Jared Brougham, Irfan Khan, Christina McKee, Mick Perez-Cruet, Rasul G Chaudhry
Intervertebral disc (IVD) degeneration is characterized by the loss of nucleus pulposus (NP), which is a common cause for lower back pain. Although, currently, there is no cure for the degenerative disc disease (DDD), stem cell therapy is increasingly being considered for its treatment. In this study, we investigated the feasibility and efficacy of human umbilical cord mesenchymal stem cells (UC-MSCs) and chondroprogenitor cells (CPCs) derived from those cells to regenerate damaged IVD in a rabbit model. Transplanted cells survived, engrafted and dispersed into nucleus pulposus (NP) in situ...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
Gauri Tendulkar, Phillip Grau, Patrick Ziegler, Alfred Buck, Alfred Buck, Andreas Badke, Hans-Peter Kaps, Sabrina Ehnert, Andreas K Nussler
Intervertebral disc degeneration and disc herniation is one of the major causes of lower back pain. Depletion of extracellular matrix, culminating in nucleus pulposus (NP) extrusion leads to intervertebral disc destruction. Currently available surgical treatments reduce the pain but do not restore the mechanical functionality of the spine. In order to preserve mechanical features of the spine, total disc or nucleus replacement thus became a wide interest. However, this arthroplasty era is still in an immature state, since none of the existing products have been clinically evaluated...
2016: Journal of Visualized Experiments: JoVE
Simon Wan, Samantha Borland, Stephen M Richardson, Catherine L R Merry, Alberto Saiani, Julie E Gough
: Cell-based therapies for regeneration of intervertebral discs are regarded to hold promise for degenerative disc disease treatment, a condition that is strongly linked to lower back pain. A de novo self-assembling peptide hydrogel (SAPH), chosen for its biocompatibility, tailorable properties and nanofibrous architecture, was investigated as a cell carrier and scaffold for nucleus pulposus (NP) tissue engineering. Oscillatory rheology determined that the system would likely be deliverable via minimally invasive procedure and mechanical properties could be optimised to match the stiffness of the native human NP...
September 24, 2016: Acta Biomaterialia
Nizar Algarni, Michael Grant, Laura M Epure, Omar Salem, Rakan Farouk Bokhari, John Antoniou, Fackson Mwale
Link N (DHLSDNYTLDHDRAIH), is a naturally occurring peptide that can stimulate matrix production in vitro, in vivo and in intact ex vivo human intervertebral discs (IVDs). We have recently discovered that annulus fibrosus cells (AF) cells have the ability to proteolytically process Link N resulting in a fragment spanning amino acid residues 1-8 short Link N (sLink N). Separately, we recently developed and validated a large animal disc organ culture model that maintains long-term cell viability while retaining the bony endplates and vertebral bone (vIVDs; IVD with intact vertebrae)...
September 27, 2016: Tissue Engineering. Part A
S Ye, B Ju, H Wang, K-B Lee
OBJECTIVES: Interleukin 18 (IL-18) is a regulatory cytokine that degrades the disc matrix. Bone morphogenetic protein-2 (BMP-2) stimulates synthesis of the disc extracellular matrix. However, the combined effects of BMP-2 and IL-18 on human intervertebral disc degeneration have not previously been reported. The aim of this study was to investigate the effects of the anabolic cytokine BMP-2 and the catabolic cytokine IL-18 on human nucleus pulposus (NP) and annulus fibrosus (AF) cells and, therefore, to identify potential therapeutic and clinical benefits of recombinant human (rh)BMP-2 in intervertebral disc degeneration...
September 2016: Bone & Joint Research
Michael F Coscia, Gerald A Denys, Matthew F Wack
STUDY DESIGN: Patients scheduled for spinal surgery were screened prospectively for a microbial presence associated with intervertebral disc specimens. Inclusion was limited to patients requiring surgery for any of 5 conditions: study patients with cervical spine intervertebral herniation (IVH), lumbar spine IVH, lumbar spine discogenic pain, and control patients with idiopathic scoliosis/Scheurermann's kyphosis or trauma/neuromuscular deformity. Exclusion criteria included ongoing systemic infection, abnormal pre-operative white cell counts, documented or suspected spinal infection, or previous surgery to the involved disc...
September 23, 2016: Spine
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