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Intervertebral disc degeneration

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https://www.readbyqxmd.com/read/29338815/correlation-between-the-expression-of-high-temperature-requirement-serine-protease-a1-in-nucleus-pulposus-tissue-and-the-degree-of-intervertebral-disc-degeneration
#1
Fan Hao Meng, Xiao Lin Shao, Yu Song, Tao Zhang
Objective To investigate the correlation between the expression level of high temperature requirement serine protease A1 (HtrA1) in nucleus pulposus and the degree of intervertebral disc degeneration (Pfirrmann grade).Methods Thirty-six patients who underwent excision of nucleus pulposus were examined by magnetic resonance imaging (MRI) before operation,and the Pfirrmann grading of all patients was performed according to the sagittal T2 weighted MRI.The expression of HtrA1 in nucleus pulposus tissue was detected by reverse transcription polymerase chain reaction and Western blot...
December 20, 2017: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
https://www.readbyqxmd.com/read/29332499/examination-of-an-in-vitro-methodology-to-evaluate-the-biomechanical-performance-of-nucleus-augmentation-in-axial-compression
#2
Sebastien Nf Sikora, Danielle E Miles, Sami Tarsuslugil, Marlène Mengoni, Ruth K Wilcox
Intervertebral disc degeneration is one of the leading causes of back pain, but treatment options remain limited. Recently, there have been advances in the development of biomaterials for nucleus augmentation; however, the testing of such materials preclinically has proved challenging. The aim of this study was to develop methods for fabricating and testing bone-disc-bone specimens in vitro for examining the performance of nucleus augmentation procedures. Control, nucleotomy and treated intervertebral disc specimens were fabricated and tested under static load...
January 1, 2018: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/29323102/propionibacterium-acnes-induces-intervertebral-disc-degeneration-by-promoting-nucleus-pulposus-cell-apoptosis-via-the-tlr2-jnk-mitochondrial-mediated-pathway
#3
Yazhou Lin, Yucheng Jiao, Ye Yuan, Zezhu Zhou, Yuehuan Zheng, Jiaqi Xiao, Changwei Li, Zhe Chen, Peng Cao
Evidence suggests that intervertebral disc degeneration (IVDD) can be induced by Propionibacterium acnes (P. acnes), although the underlying mechanisms are unclear. In this study, we analyzed the pathological changes in degenerated human intervertebral discs (IVDs) infected with P. acnes. Compared with P. acnes-negative samples, P. acnes-positive IVDs showed increased apoptosis of nucleus pulposus cells (NPCs) concomitant with severe IVDD. Then, a P. acnes-inoculated IVD animal model was established, and severe IVDD was induced by P...
January 10, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29320615/an-intervertebral-disc-whole-organ-culture-system-to-investigate-proinflammatory-and-degenerative-disc-disease-condition
#4
Gernot Lang, Yishan Liu, Janna Geries, Zhiyu Zhou, David Kubosch, Norbert Südkamp, R Geoff Richards, Mauro Alini, Sibylle Grad, Zhen Li
OBJECTIVE: The aim of this study was to compare the effect of different disease initiators of degenerative disc disease (DDD) within an intervertebral disc (IVD) organ culture system, and to understand the interplay between inflammation and degeneration in the early stage of DDD. DESIGN: Bovine caudal IVDs were cultured within a bioreactor for up to 11 days. Control group was cultured under physiological loading (0.02-0.2 MPa; 0.2 Hz; 2h/day) and high glucose (4...
January 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29319363/expression-of-serglycin-in-human-disc-is-increased-in-degenerated-discs-and-up-regulated-in-vitro-by-exposure-to-il-1%C3%A3-or-tnf-%C3%AE
#5
H E Gruber, E N Hanley
We investigated whether the multifunctional intercellular proteoglycan, serglycin, is expressed in human intervertebral disc cells and assessed its localization. We also investigated expression levels of serglycin in human annulus fibrosus (annulus) cells exposed to IL-1ß and TNF-α, which are two proinflammatory cytokines that are expressed during disc degeneration. Immunolocalization of serglycin was common in many cells of the human annulus, but less common in the nucleus pulposus (nucleus). Both intracellular and cell membrane localization were observed...
January 10, 2018: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/29316495/vdr-and-gc-gene-polymorphisms-modulate-the-risk-of-lumbar-disc-degeneration-in-iran
#6
Sohail Mashayekhi, Alia Saberi, Zivar Salehi, Gelareh Biazar, Roghayeh Mehrdel
OBJECTIVE: Lumbar disc degeneration (LDD) occurs commonly in humans. Vitamin D metabolic and signaling pathway plays a significant role in intervertebral disc degeneration. The aim of this study was to evaluate the influence of the genetic polymorphism in the two key genes of 1,25-(OH)2-D3 pathway, VDR (vitamin D receptor) and GC (group-specific component), in LDD development. PATIENTS AND METHODS: Two single-nucleotide polymorphisms, VDR rs2228570 (FokI) and GC rs7041, were genotyped in 180 patients with LDD and 230 healthy individuals...
December 29, 2017: Clinical Neurology and Neurosurgery
https://www.readbyqxmd.com/read/29315127/tidemark-avulsions-are-a-predominant-form-of-endplate-irregularity
#7
Britta Berg-Johansen, Deeptee Jain, Ellen C Liebenberg, Aaron J Fields, Thomas M Link, Conor W O'Neill, Jeffrey C Lotz
STUDY DESIGN: Descriptive histologic and MRI study of human cadaveric spines. OBJECTIVE: To identify and characterize common endplate pathologies to form a histologic foundation for an etiology-based classification system. SUMMARY OF BACKGROUND DATA: Irregularities at the spinal disc-vertebra interface are associated with back pain and intervertebral disc herniation injuries. However, there is currently a lack of consensus regarding terminology for classification...
January 8, 2018: Spine
https://www.readbyqxmd.com/read/29314724/genipin-cross-linked-type-ii-collagen-scaffold-promotes-the-differentiation-of-adipose-derived-stem-cells-into-nucleus-pulposus-like-cells
#8
Xiaopeng Zhou, Yiqing Tao, Erman Chen, Jingkai Wang, Weijing Fang, Tengfei Zhao, Chengzhen Liang, Fangcai Li, Qixin Chen
Adipose-derived stem cells (ADSCs)-based tissue engineering was a promising method to treat intervertebral disc degeneration. Type II collagen is a native component in the nucleus pulposus (NP), and has the ability to promote ADSCs to differentiate into NP-like cells. In this article, we aimed to establish a genipin-cross-linked three-dimensional (3D) type II collagen scaffold, and determine the biological effects of the scaffold on ADSCs differentiating into a NP-like phenotype. Different concentrations of genipin were used to cross-link the 3D type II collagen scaffold...
January 3, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29314237/nondestructive-indirect-assessment-of-the-biomechanical-properties-of-the-rat-intervertebral-disc-using-contrast-enhanced-%C3%A2%C2%B5ct
#9
Michael D Newton, Samantha E Hartner, Karissa Gawronski, Erik J Davenport, Shannon C Timmons, Kevin C Baker, Tristan Maerz
Mechanical characterization of the intervertebral disc involves labor-intensive and destructive experimental methodology. Contrast-enhanced micro-computed tomography is a nondestructive imaging modality for high-resolution visualization and glycosaminoglycan quantification of cartilaginous tissues. The purpose of this study was to determine whether anionic and cationic contrast-enhanced micro-computed tomography of the intervertebral disc can be used to indirectly assess disc mechanical properties in an ex vivo model of disc degeneration...
January 4, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/29312639/progranulin-derived-engineered-protein-atsttrin-suppresses-tnf-%C3%AE-mediated-inflammation-in-intervertebral-disc-degenerative-disease
#10
Hong Ding, Jianlu Wei, Yunpeng Zhao, Yi Liu, Lian Liu, Lei Cheng
Atsttrin, an engineered molecule composed of three fragments of progranulin(PGRN), exerts comparable anti-inflammation ability. Intervertebral disc degeneration (IDD) is involved in inflammation in which TNF-α plays a key role. This study aims to examine the effect and the mechanism of Atsttrin in the pathogenesis of intervertebral disc degeneration. For this purpose, we took advantage of murine and human intervertebral disc (IVD) and examined the expression of TNF-α in IVD tissues using immunohistochemistry and TNF-α level in peripheral sera by ELISA assay...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29307048/biomechanical-analysis-of-a-new-lumbar-interspinous-device-with-optimized-topology
#11
Chen-Sheng Chen, Shih-Liang Shih
Interspinous spacers used stand-alone preserve joint movement but provide little protection for diseased segments of the spine. Used as adjuncts with fusion, interspinous spacers offer rigid stability but may accelerate degeneration on adjacent levels. Our new device is intended to balance the stability and preserves motion provided by the implant. A new interspinous spacer was devised according to the results of topology optimization studies. Four finite element (FE) spine models were created that consisted of an intact spine without an implant, implantation of the novel, the device for intervertebral assisted motion (DIAM system), and the Dynesys system...
January 6, 2018: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29304802/evaluation-of-the-influence-of-kyphosis-and-scoliosis-on-intervertebral-disc-extrusion-in-french-bulldogs
#12
Maria Claudia C M Inglez de Souza, Richard Ryan, Gert Ter Haar, Rowena M A Packer, Holger A Volk, Steven De Decker
BACKGROUND: Although thoracic vertebral malformations with kyphosis and scoliosis are often considered incidental findings on diagnostic imaging studies of screw-tailed brachycephalic breeds, they have been suggested to interfere with spinal biomechanics and intervertebral disc degeneration. It is however unknown if an abnormal spinal curvature also predisposes dogs to develop clinically relevant intervertebral disc herniations. The aim of this study was to evaluate if the occurrence of thoracic vertebral malformations, kyphosis or scoliosis would be associated with a higher prevalence of cervical or thoracolumbar intervertebral disc extrusion in French bulldogs...
January 5, 2018: BMC Veterinary Research
https://www.readbyqxmd.com/read/29301464/intervertebral-disc-degeneration-in-warmblood-horses-morphology-grading-and-distribution-of-lesions
#13
Wilhelmina Bergmann, Niklas Bergknut, Stefanie Veraa, Andrea Gröne, Hans Vernooij, Inge D Wijnberg, Willem Back, Guy C M Grinwis
Equine intervertebral disc degeneration is thought to be rare and of limited clinical relevance, although research is lacking. To objectively assess pathological changes of the equine intervertebral disc and their clinical relevance, description of the normal morphology and a practical, biologically credible grading scheme are needed. The objectives of this study are to describe the gross and histological appearance of the equine intervertebral discs and to propose a grading scheme for macroscopic degeneration...
January 1, 2018: Veterinary Pathology
https://www.readbyqxmd.com/read/29299204/downregulation-of-microrna-125a-is-involved-in-intervertebral-disc-degeneration-by-targeting-pro-apoptotic-bcl-2-antagonist-killer-1
#14
Ping Liu, Feng Chang, Ting Zhang, Gang Gao, Chen Yu, Sheng-Qiang Ding, Gen-Le Zuo, Xin-Hu Huang
Objectives: To investigate the role of the microRNA-125a (miR-125a) and BAK1 in intervertebral disc degeneration (IDD). Materials and Methods: Degenerative lumbar nucleus pulposus (NP) tissues were obtained from 193 patients who underwent resection, and normal controls consisted of normal NP tissues from 32 patients with traumatic lumbar fracture in our hospital. All patients were graded according to the Pfirrmann criteria. QRT-PCR was used to detect the expression of miR-125a and BAK1, and apoptosis of NP tissues detected by TUNEL staining...
November 2017: Iranian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/29284457/trail-gene-1595c-t-polymorphisms-contribute-to-the-susceptibility-and-severity-of-intervertebral-disc-degeneration-a-data-synthesis
#15
Qi-Ling Yuan, Liang Liu, Yong-Song Cai, Yin-Gang Zhang
BACKGROUND: Studies have investigated the correlation between tumor necrosis factor related apoptosis-inducing ligand (TRAIL) gene polymorphisms and the susceptibility and severity of intervertebral disc degeneration (IDD), but the results were inconsistent. To evaluate the specific relationship, we performed a meta-analysis to clarify the controversies. METHODS: Four databases were searched, and the pooled results were presented as odds ratios (ORs) with 95% confidence intervals (CIs)...
December 29, 2017: BMC Musculoskeletal Disorders
https://www.readbyqxmd.com/read/29284234/chemometric-evaluation-of-concentrations-of-trace-elements-in-intervertebral-disc-tissue-in-patient-with-degenerative-disc-disease
#16
Łukasz Kubaszewski, Anetta Zioła-Frankowska, Zuzanna Gasik, Marcin Frankowski, Mikołaj Dąbrowski, Bartłomiej Molisak, Jacek Kaczmarczyk, Robert Gasik
BACKGROUND: The work is designed to uncover the pattern of mutual relation among trace elements and epidemiological data in the degenerated intervertebral disk tissue in humans. Hitherto the reason of the degenerative process is not fully understood. Trace elements are the basic components of the biological compound related both its metabolism as well as environmental exposure. The relation pattern among elements occurs gives new perspective in solving the cause of the disease. MATERIAL AND METHODS: We have analysed trace elements content in the 30 intervertebral disc from 22 patients with degenerative disc disease...
December 23, 2017: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/29279740/detection-of-o-linked-n-acetylglucosamine-modification-and-its-associated-enzymes-in-human-degenerated-intervertebral-discs
#17
Georgios Nikolaou, Aristeidis H Zibis, Apostolos H Fyllos, Antonios Katsioulis, Sotirios Sotiriou, Anastasios Kotrotsios, Markos Sgantzos, Aikaterini Vassiou, Dimitrios L Arvanitis
Study Design: Human herniated discs were obtained from discectomy specimens for the immunohistochemical detection of O-GlcNAc and O-GlcNAcase (OGA)/O-GlcNAc transferase (OGT). Purpose: This study aimed to quantify the extent of O-GlcNAcylation and its associated enzymes (OGT/OGA) in human degenerated intervertebral discs. Overview of Literature: The O-GlcNAcylation of nuclear, cytoplasmic, and mitochondrial proteins as well as the effects of such post-translational modifications are currently the focus of extensive research...
December 2017: Asian Spine Journal
https://www.readbyqxmd.com/read/29259641/icariin-prevents-il-1%C3%AE-induced-apoptosis-in-human-nucleus-pulposus-via-the-pi3k-akt-pathway
#18
Xiangyu Deng, Wei Wu, Hang Liang, Donghua Huang, Doudou Jing, Dong Zheng, Zengwu Shao
Purpose: To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that was applied to IL-1β pretreated human nucleus pulposus (NP) cells. Methods: Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis. The cells were divided into five groups: A (blank control); B (20 ng/ml IL-1β); C (20 ng/ml IL-1β + 20 μM icariin); D (20 μM icariin + 20 ng/ml IL-1β + 25 μM LY294002); E (20 ng/ml IL-1β + 25 μM LY294002)...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29258934/lentivirus-is-an-efficient-and-stable-transduction-vector-for-intervertebral-disc-cells
#19
Yue-Hui Zhang, Yang-Lu Zhao, Bo Li, Jia Song, Jing Zhang, Jiang Shao
OBJECTIVES: To evaluate the transduction efficacy and sustainability of lentiviral vector for intervertebral disc cells, both in vitro and in vivo. METHORDS: Human nuclear pulposus and annulus fibrosus cells isolated from disc tissue of 28 patients during surgical disc procedures were cultured, and subsequently transduced using recombinant lentivirus carrying a gene for enhanced green fluorescent protein (EGFP) at multiplicities of infection (MOI) of 0, 15, 30, 60, 90, and 150...
December 16, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29250209/spine-on-a-chip-human-annulus-fibrosus-degeneration-model-for-simulating-the-severity-of-intervertebral-disc%C3%A2-degeneration
#20
Min Ho Hwang, Dong Hyun Cho, Seung Min Baek, Jae Won Lee, Jeong Hun Park, Chang Min Yoo, Jae Hee Shin, Hyo Geun Nam, Hyeong Guk Son, Hyun Jung Lim, Han Sang Cho, Hong Joo Moon, Joo Han Kim, Jong Kwang Lee, Hyuk Choi
The aetiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no effective fundamental therapies. Symptomatic IVD is known to be associated with nerve root compression. However, even in the absence of nerve compression, LBP occurs in patients with IVD degeneration. We hypothesize that this phenomenon is associated with a concentration of pro-inflammatory cytokines such as interleukin (IL)-1β and tumour necrosis factor-alpha (TNF-α), which can lead to altered histologic features and cellular phenotypes observed during IVD degeneration...
November 2017: Biomicrofluidics
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