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Intervertebral disc degeneration

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https://www.readbyqxmd.com/read/28544701/intradiscal-application-of-a-pcla-peg-pcla-hydrogel-loaded-with-celecoxib-for-the-treatment-of-back-pain-in-canines-what-s-in-it-for-humans
#1
Anna R Tellegen, Nicole Willems, Martijn Beukers, Guy C M Grinwis, Saskia G M Plomp, Clemens Bos, Maarten van Dijk, Mike de Leeuw, Laura B Creemers, Marianna A Tryfonidou, Björn P Meij
Chronic low back pain is a common clinical problem in both the human and canine population. Current pharmaceutical treatment often consists of oral anti-inflammatory drugs to alleviate pain. Novel treatments for degenerative disc diseases focus on local application of sustained released drug formulations. The aim of this study was to determine safety and feasibility of intradiscal application of a PCLA-PEG-PCLA hydrogel releasing celecoxib, a COX-2 inhibitor. Biocompatibility was evaluated after subcutaneous injection in mice and safety of intradiscal injection of the hydrogel was evaluated in experimental dogs with early spontaneous intervertebral disc (IVD) degeneration...
May 19, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28543639/lncrna-rp11-296a18-3-mir-138-hif1a-pathway-regulates-the-proliferation-ecm-synthesis-of-human-nucleus-pulposus-cells-hnpcs-%C3%A2
#2
Xiaobin Wang, Guohua Lv, Jing Li, Bing Wang, Qianshi Zhang, Chang Lu
BACKGROUND: During the process of Intervertebral disc degeneration (IDD), nucleus pulposus apoptosis increases, extracellular matrix (ECM) alters and/or degrades, abnormal proliferation of cells forms cell clusters, and the expression of various inflammatory factors increases. Thus, regulation of human nucleus pulposus cell (HNPC) proliferation and ECM synthesis present promising strategies for IDD treatment. Accumulating evidence indicates that non-coding RNAs are involved in various cellular processes, including cell proliferation, differentiation, apoptosis and metastasis...
May 24, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28542098/comparison-of-oxygen-consumption-rates-of-nondegenerate-and-degenerate-human-ivd-cells
#3
Sarah E Cisewski, Yongren Wu, Brooke J Damon, Barton L Sachs, Michael J Kern, Hai Yao
STUDY DESIGN: In vitro measurements of the oxygen consumption rates (OCR) of human intervertebral disc (IVD) cells. OBJECTIVE: To determine the differences in the OCR of nondegenerate and degenerate human annulus fibrosus (AF), nucleus pulposus (NP), and cartilage endplate (CEP) cells at different glucose concentrations. SUMMARY OF BACKGROUND DATA: The avascular nature of the IVD creates a delicate balance between rate of nutrient transport through the matrix and rate of disc cell consumption necessary to maintain tissue health...
May 24, 2017: Spine
https://www.readbyqxmd.com/read/28541819/model-of-disc-degeneration-in-rat-tail-induced-through-a-vascular-isolation-of-vertebral-endplates
#4
Héctor Fernández-Susavila, Juan Pablo Pardo-Seco, Ramón Iglesias-Rey, Tomás Sobrino, Francisco Campos, Máximo Alberto Díez-Ulloa
Back pain is a major health problem. The degenerative cascade of the spine begins in the intervertebral disc, due to an impairment in the blood supply through the vertebral endplates. Our objective was to develop a novel disc degeneration model based on these premises, akin to the process in humans, in contrast to other proposed models (puncture, enzyme injection, aberrant loads,…) Material and methods: 37 Sprague-Dawley rats, 2 arms: (a) histological (n = 17, one died), en- bloc sections, Van Gieson staining, (Nisimura-Mochida criteria) and also collagen VI staining (tissue oxidative stress), four animals were euthanized every 2 weeks (2-8); and (b) imaging (n = 20, six wound sloughs), MRI 9...
May 25, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28537499/vitamin-d-improves-the-content-of-tgf-%C3%AE-and-igf-1-in-intervertebral-disc-of-diabetic-rats
#5
Ji-Long An, Wei Zhang, Jian Zhang, Li-Chao Lian, Yong Shen, Wen-Yuan Ding
OBJECTIVE: The contents of transforming growth factor-β and insulin-like growth factor-1 in disc of diabetic rats were measured at three different periods after injected with 1,25-Dihydroxyvitamin D3, and compared with that in normal rats. The significance of content changes was also discussed. METHODS: Fourty-five Sprague-Dawley (SD) rats were divided into three groups, namely the experimental group (STZ+calcitriol), control group (STZ+citrate buffer), and normal group (citrate buffer)...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28526405/hypoxia-inducible-factor-l%C3%AE-mediates-aggrecan-and-collagen-%C3%AE-expression-via-notch1-signaling-in-nucleus-pulposus-cells-during-intervertebral-disc-degeneration
#6
Zhuochao Liu, Changwei Li, Xiangchao Meng, Yunting Bai, Jin Qi, Jun Wang, Qi Zhou, Weibin Zhang, Xingkai Zhang
Although hypoxia-inducible factor-lα (HIF-lα) has been reported to have an important role in the metabolism and synthesis of the extracellular matrix (ECM) of nucleus pulposus cells (NPCs), the underlying mechanism has not been fully clarified. Here, we show for the first time that NOTCH1 expression is decreased in NPs isolated from degenerated human intervertebral discs (IVDs), as well as in the NPs of NP-specific HIF-1α(-/-) mice. Our study reveals that overexpression of HIF-1α leads to increased expression of NOTCH1, the NOTCH1 ligand JAGGED1, and its target gene hairy and enhancer of split-1 (HES1), while also upregulating collagen Π and aggrecan expression in human NPCs...
May 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28525292/comparison-of-adjacent-segment-degeneration-after-nonrigid-fixation-system-and-posterior-lumbar-interbody-fusion-for-single-level-lumbar-disc-herniation-a-new-method-of-mri-analysis-of-lumbar-nucleus-pulposus-volume
#7
Shaofeng Yang, Yanan Liu, Zhaohua Bao, Jun Zou, Huilin Yang
PURPOSE: To evaluate the influence of a nonrigid fixation system and posterior lumbar interbody fusion on adjacent intervertebral disc degeneration by using MRI analysis of lumbar nucleus pulposus volume for single-level lumbar disc herniation. MATERIALS AND METHODS: We selected 112 patients who underwent nonrigid fixation (17 men and 44 women) or posterior lumbar interbody fusion (13 men and 38 women) for this retrospective study. Based on the T2-weighted magnetic resonance imaging (MRI) scans taken preoperatively, and 6, 12, and 24 months after surgery, the nucleus pulposus in the upper segments of the operated level was considered an ellipsoid, and their volumes were measured respectively and then compared between the two groups...
May 19, 2017: Journal of Investigative Surgery: the Official Journal of the Academy of Surgical Research
https://www.readbyqxmd.com/read/28514734/inflammatory-microrna-194-and-515-attenuate-the-biosynthesis-of-chondroitin-sulfate-during-human-intervertebral-disc-degeneration
#8
Bo Hu, Chen Xu, Ye Tian, Changgui Shi, Ying Zhang, Lianfu Deng, Hongyu Zhou, Peng Cao, Huajiang Chen, Wen Yuan
Intervertebral disc degeneration (IDD) is characterized by dehydration and loss of extracellular matrixes in the nucleus pulposus region. Chondroitin sulfate has been found to be the water-binding molecule that played a key role in IDD. Although investigators have reported that inflammatory cytokines are involved in the reduction of chondroitin sulfate in IDD, but the underlying mechanism is unrevealed. Since chondroitin sulfate synthesis is controlled by chondroitin sulfate glycosyltransferases CHSY-1/2/3 and CSGALNACT-1/2, their functional role and regulatory mechanism in IDD is not fully studied...
May 2, 2017: Oncotarget
https://www.readbyqxmd.com/read/28512264/gallic-acid-inhibits-the-release-of-adamts4-in-nucleus-pulposus-cells-by-inhibiting-p65-phosphorylation-and-acetylation-of-the-nf-%C3%AE%C2%BAb-signaling-pathway
#9
Yao Huang, Jian Chen, Tao Jiang, Zheng Zhou, Bin Lv, Guoyong Yin, Jin Fan
This study investigated the inhibitory effect of gallic acid (GA) on the release of A Disintegrin and Metalloproteinase with Thrombospondin motifs 4 (ADAMTS4) through the regulation of the NF-κB signaling pathway, which is closely related to the matrix metalloproteinases in nucleus pulposus cells. Different concentrations of GA were added to TNF-α-induced human nucleus pulposus cells (hNPCs) and intervertebral disc degeneration rat model. ADAMTS-4 expression increased both in the TNF-α-induced nucleus pulposus cells and intervertebral disc degeneration rat model...
April 28, 2017: Oncotarget
https://www.readbyqxmd.com/read/28509587/link-protein-n-terminal-peptide-and-fullerol-promote-matrix-production-and-decrease-degradation-enzymes-in-rabbit-annulus-cells
#10
Ching-Hua Yeh, Dennis Chen, Bayan Aghdasi, Li Xiao, Mengmeng Ding, Li Jin, Xudong Li
PURPOSE: Intervertebral disc degeneration is a major cause of back pain. Novel therapies for prevention or reversal of disc degeneration are needed. It is desirable for potential therapies to target both inflammation and matrix degeneration. MATERIALS AND METHODS: The combined regenerative potential of link protein N-terminal peptide (LN) and fullerol on annulus fibrosus (AF) cells was evaluated in a 3D culture model. RESULTS: IL-1α-induced AF cell degeneration was counteracted by fullerol, LN, and fullerol + LN, with the latter having the greatest effect on matrix production as evaluated by real-time PCR and GAG assay...
May 16, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28507882/aquaporin-1-expression-in-herniated-human-lumbar-intervertebral-discs
#11
Haydn Hoffman, Aaron W Choi, Victor Chang, Jon Kimball, Alan S Verkman, Rubeen Virani, Brian Kim, Tianyi Niu, Daniel C Lu
STUDY DESIGN: Case series. OBJECTIVE: Intervertebral disc (IVD) degeneration is the cause of spondylosis. The pathogenesis is poorly understood, but disc dehydration often plays a role. In this study, we aim to identify and quantify aquaporin-1 (AQP1) in ex vivo human degenerated IVDs obtained intraoperatively and to investigate the relationship between AQP1 levels and magnetic resonance imaging (MRI) T2 intensity of the disc. METHODS: Ex vivo samples of nucleus pulposus (NP) tissue from lumbar IVDs were obtained from 18 consecutive patients who underwent surgery for disc herniation at L4/5 and L5/S1 level...
April 2017: Global Spine Journal
https://www.readbyqxmd.com/read/28505960/normal-aging-of-the-lumbar-spine-in-women
#12
Hulya Kurtul Yildiz, Elif Evrim Ekin
BACKGROUND: Lumbar lordosis is required for bipedalism. OBJECTIVES: To investigate age-related changes in lumbar lordosis and to clarify the relationships between lumbar lordosis and vertebral wedging and disc degeneration. METHODS: A total of 300 women were included in this retrospective study, 50 in each of six age groups (20-, 30-, 40-, 50-, 60- and 70-year-olds). Patients with vertebral collapse, instable fracture or disc sequestration were excluded...
May 5, 2017: Journal of Back and Musculoskeletal Rehabilitation
https://www.readbyqxmd.com/read/28501720/characterization-and-restoration-of-degenerated-ivd-function-with-an-injectable-in-situ-gelling-alginate-hydrogel-an-in-vitro-and-ex-vivo-study
#13
Emily A Growney Kalaf, Meghana Pendyala, J Gary Bledsoe, Scott A Sell
Intervertebral disc (IVD) degeneration is a naturally, irreparable process that causes loss in IVD hydration, cellularity, and, mechanical stability. Invasive surgical attempts to ease back pain and, radiculopathy have shown to cause increased degeneration along the rest, of the spine. Due to its highly tunable mechanical and degradation, properties, alginate is a viable option for a less-invasive injectable, repair for IVD degeneration. This study centers on the characterization, of in situ gelling alginate and subsequent injection into enzymatically, degraded motion segments...
May 7, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28500930/micro-computed-tomography-scanning-electron-microscopy-and-energy-x-ray-spectroscopy-studies-of-facet-joint-degeneration-a-comparison-to-clinical-imaging
#14
Felix Goehre, Christopher Ludtka, Melanie Hamperl, Andrea Friedmann, Anja Straube, Thomas Mendel, Andreas Heilmann, Hans Jörg Meisel, Stefan Schwan
Segmental degeneration in the human lumbar spine affects both the intervertebral discs and facet joints. Facet joint degeneration not only affects the cartilage surface, but also alters the cellular properties of the cartilage tissue and the structure of the subchondral bone. The primary focus of this study is the investigation of these microstructural changes that are caused by facet joint degeneration. Microstructural analyses of degenerated facet joint samples, obtained from patients following operative lumbar interbody fusion, have not previously been extensively investigated...
May 1, 2017: Micron: the International Research and Review Journal for Microscopy
https://www.readbyqxmd.com/read/28498809/follistatin-like-protein-1-promotes-inflammatory-reactions-in-nucleus-pulposus-cells-by-interacting-with-the-mapk-and-nf%C3%AE%C2%BAb-signaling-pathways
#15
Yi Liu, Jianlu Wei, Yunpeng Zhao, Yuanqiang Zhang, Yingguang Han, Bin Chen, Kaiyuan Cheng, Jialin Jia, Lin Nie, Lei Cheng
OBJECTIVE: Follistatin-like protein 1 (FSTL1) is a well-known mediator of inflammation. Intervertebral disc disease is an inflammatory disorder. Here, we investigated the role of FSTL1 in the intervertebral discs inflammation. METHODS: Expression of FSTL1 in nucleus pulposus tissues from rats and human was determined by immunohistochemistry staining and western blot analysis. The expression levels of tumor necrosis factor-α (TNF-α), interleukin1-β (IL-1β) and matrix metalloproteinase 13 (MMP-13) in human and rat nucleus pulposus tissues were measured by immunohistochemistry staining...
April 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28498326/a-histopathological-scheme-for-the-quantitative-scoring-of-intervertebral-disc-degeneration-and-the-therapeutic-utility-of-adult-mesenchymal-stem-cells-for-intervertebral-disc-regeneration
#16
Cindy C Shu, Margaret M Smith, Susan M Smith, Andrew J Dart, Christopher B Little, James Melrose
The purpose of this study was to develop a quantitative histopathological scoring scheme to evaluate disc degeneration and regeneration using an ovine annular lesion model of experimental disc degeneration. Toluidine blue and Haematoxylin and Eosin (H&E) staining were used to evaluate cellular morphology: (i) disc structure/lesion morphology; (ii) proteoglycan depletion; (iii) cellular morphology; (iv) blood vessel in-growth; (v) cell influx into lesion; and (vi) cystic degeneration/chondroid metaplasia...
May 12, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28498027/asthma-and-early-herniated-intervertebral-disc-disease
#17
Cheng-Di Chiu, Hsuan-Ju Chen, Hean-Pat Saw, Nai-Wei Yao, Hung-Rong Yen, Chia-Hung Kao
BACKGROUND: The etiology of herniated intervertebral disc (HIVD) disease in children and adolescents is multifactorial and not merely related to disc degeneration. Therefore, in the present study, we investigated the relationship between young asthma patients and the risk of early HIVD disease in a population under 30 years of age. MATERIALS AND METHODS: Data from the National Health Insurance Research Database (NHIRD) of Taiwan were used to conduct a retrospective longitudinal cohort study...
May 12, 2017: Current Medical Research and Opinion
https://www.readbyqxmd.com/read/28497435/genetic-dimension-of-intervertebral-disc-degeneration-polymorphism-of-matrix-metalloproteinase-1-and-3-in-the-north-iranian-population
#18
Alia Saberi, Zivar Salehi, Bahram Naderinabi, Sahar Hojaat Ansari, Sohail Mashayekhi
AIM: Exploring the relationship between two polymorphisms (MMP-1-755 T/G [rs498186] and MMP-3 A/C [rs632478]) and disc degeneration. MATERIAL AND METHODS: We performed a case-control study on 130 cases with intervertebral disc degeneration confirmed by Magnetic Resonance Imaging (MRI) and 210 healthy individuals. Schneiderman criterion was used to determine the severity of the disc degeneration. Blood samples were collected from the participants. The genotyping was performed by polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method...
April 4, 2017: Turkish Neurosurgery
https://www.readbyqxmd.com/read/28495754/phd3-is-a-transcriptional-coactivator-of-hif-1%C3%AE-in-nucleus-pulposus-cells-independent-of-the-pkm2-jmjd5-axis
#19
Zachary R Schoepflin, Elizabeth S Silagi, Irving M Shapiro, Makarand V Risbud
The role of prolyl hydroxylase (PHD)-3 as a hypoxia inducible factor (HIF)-1α cofactor is controversial and remains unknown in skeletal tissues. We investigated whether PHD3 controls HIF-1 transcriptional activity in nucleus pulposus (NP) cells through the pyruvate kinase muscle (PKM)-2-Jumonji domain--containing protein (JMJD5) axis. PHD3(-/-) mice (12.5 mo old) showed increased incidence of intervertebral disc degeneration with a concomitant decrease in expression of the HIF-1α targets VEGF-A, glucose transporter-1, and lactate dehydrogenase A...
May 11, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28486958/exosomes-as-potential-alternatives-to-stem-cell-therapy-for-intervertebral-disc-degeneration-in-vitro-study-on-exosomes-in-interaction-of-nucleus-pulposus-cells-and-bone-marrow-mesenchymal-stem-cells
#20
Kang Lu, Hai-Yin Li, Kuang Yang, Jun-Long Wu, Xiao-Wei Cai, Yue Zhou, Chang-Qing Li
BACKGROUND: The stem cell-based therapies for intervertebral disc degeneration have been widely studied. However, the mechanisms of mesenchymal stem cells interacting with intervertebral disc cells, such as nucleus pulposus cells (NPCs), remain unknown. Exosomes as a vital paracrine mechanism in cell-cell communication have been highly focused on. The purpose of this study was to detect the role of exosomes derived from bone marrow mesenchymal stem cells (BM-MSCs) and NPCs in their interaction with corresponding cells...
May 10, 2017: Stem Cell Research & Therapy
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