keyword
MENU ▼
Read by QxMD icon Read
search

F-actin

keyword
https://www.readbyqxmd.com/read/29027931/discovering-the-biological-target-of-5-epi-sinuleptolide-using-a-combination-of-proteomic-approaches
#1
Elva Morretta, Roberta Esposito, Carmen Festa, Raffaele Riccio, Agostino Casapullo, Maria Chiara Monti
Sinuleptolide and its congeners are diterpenes with a norcembranoid skeleton isolated from the soft coral genus Sinularia. These marine metabolites are endowed with relevant biological activities, mainly associated with cancer development. 5-epi-sinuleptolide has been selected as a candidate for target discovery studies through the application of complementary proteomic approaches. Specifically, a combination of conventional chemical proteomics based on affinity chromatography, coupled with high-resolution mass spectrometry and bioinformatics, as well as drug affinity responsive target stability (DARTS), led to a clear identification of actins as main targets for 5-epi-sinuleptolide...
October 13, 2017: Marine Drugs
https://www.readbyqxmd.com/read/29027376/adenosine-a2a-receptor-agonist-ameliorates-eae-and-correlates-with-th1-cytokine-induced-blood-brain-barrier-dysfunction-via-suppression-of-mlck-signaling-pathway
#2
Ying Liu, Marwan Alahiri, Bianca Ulloa, Boxun Xie, Saud A Sadiq
INTRODUCTION: Multiple sclerosis (MS) disease activity is associated with blood-brain barrier (BBB) disruption, which is mediated by inflammatory cytokines released by CD4+ lymphocytes. To assess the effects of adenosine A2A receptors on BBB permeability in vitro and in vivo. METHODS: A2A receptor expression was detected by immunostaining in experimental autoimmune encephalomyelitis (EAE) C57BL/6 mice immunized with myelin oligodendrocyte glycoprotein (MOG)35-55 , and human MS brain...
October 12, 2017: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/29025914/dendritic-cytoskeletal-architecture-is-modulated-by-combinatorial-transcriptional-regulation-in-drosophila-melanogaster
#3
Ravi Das, Shatabdi Bhattacharjee, Atit A Patel, Jenna M Harris, Surajit Bhattacharya, Jamin M Letcher, Sarah G Clark, Sumit Nanda, Eswar Prasad R Iyer, Giorgio A Ascoli, Daniel N Cox
Transcription factors (TFs) have emerged as essential cell autonomous mediators of sub-type specific dendritogenesis, however, the downstream effectors of these TFs remain largely unknown, as are the cellular events that TFs control to direct morphological change. As dendritic morphology is largely dictated by the organization of the actin and microtubule (MT) cytoskeletons, elucidating TF-mediated cytoskeletal regulatory programs is key to understanding molecular control of diverse dendritic morphologies. Previous studies in Drosophila melanogaster have demonstrated that the conserved TFs Cut and Knot exert combinatorial control over aspects of dendritic cytoskeleton development, promoting actin- and MT-based arbor morphology, respectively...
October 12, 2017: Genetics
https://www.readbyqxmd.com/read/29024522/in-vitro-safety-evaluation-of-human-nasal-epithelial-cell-monolayers-exposed-to-carrageenan-sinus-wash
#4
Mahnaz Ramezanpour, Jae Murphy, Jason L P Smith, Sarah Vreugde, Alkis James Psaltis
BACKGROUND: Carrageenans have shown to reduce the viral load in nasal secretions and lower the incidence of secondary infections in children with common cold. Despite the widespread use of carrageenans in topical applications, the effect of carrageenans on the sinonasal epithelial barrier has not been elucidated. We investigate the effect of different carrageenans on the sinonasal epithelial barrier and inflammatory response in vitro. METHODS: Iota and Kappa carrageenan delivered in saline irrigation solutions applied to air-liquid interface (ALI) cultures of primary human nasal epithelial cells from chronic rhinosinusitis patients and controls...
October 12, 2017: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/29023577/sevoflurane-protects-rat-brain-endothelial-barrier-structure-and-function-after-hypoxia-reoxygenation-injury
#5
Tanja Restin, Marie-Elisabeth Kajdi, Martin Schläpfer, Birgit Roth Z'graggen, Christa Booy, Claudia Dumrese, Beatrice Beck-Schimmer
BACKGROUND: After cerebral injury blood-brain barrier disruption significantly impairs brain homeostasis. Volatile anesthetics have been shown to be protective in ischemia-reperfusion injury scenarios. Their impact on brain endothelial cells after hypoxia-reoxygenation (H/R) has not yet been studied in detail. METHODS: Rat brain endothelial cells (RBE4) were exposed to severe hypoxia and reoxygenated in air in the presence or absence of sevoflurane. Changes in dextran permeability and architecture of the cellular junctional proteins ZO-1 and β-catenin were measured...
2017: PloS One
https://www.readbyqxmd.com/read/29018992/tenocyte-proliferation-and-migration-promoted-by-rat-bone-marrow-mesenchymal-stem-cell-derived-conditioned-medium
#6
Qiufang Chen, Qingfei Liang, Weixia Zhuang, Jun Zhou, Bingyu Zhang, Pu Xu, Yang Ju, Yasuyuki Morita, Qing Luo, Guanbin Song
OBJECTIVES: To investigate the impact of secreted factors of rat bone marrow mesenchymal stem cells (MSCs) on the proliferation and migration of tenocytes and provide evidence for the development of MSC-based therapeutic methods of tendon injury. RESULTS: Rat bone marrow mesenchymal stem cell-derived conditioned medium (MSC-CM) promoted the proliferation of tenocytes within 24 h and decreased the percentage of tenocytes in G1 phase. MSC-CM activated the extracellular signal-regulated kinase1/2 (ERK1/2) signal molecules, while the ERK1/2 inhibitor PD98059 abrogated the MSC-CM-induced proliferation of tenocytes, decreased the fraction of tenocytes in the G1 phase and elevated p-ERK1/2 expression...
October 10, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/29018403/repulsive-guidance-molecule-a-inhibits-angiogenesis-by-downregulating-vegf-and-phosphorylated-focal-adhesion-kinase-in-vitro
#7
Gang Zhang, Rong Wang, Ke Cheng, Qi Li, Yu Wang, Rongrong Zhang, Xinyue Qin
Repulsive guidance molecule a (RGMa) is a major neuron guidance factor in central nervous systems. We previously found that inhibition of RGMa could greatly enhance neural function rehabilitation in rats after MCAO/reperfusion. Neuron guidance factors are often regulators of angiogenesis. However, the effect of RGMa on angiogenesis and its mechanisms remain to be determined. Here, we investigated the effect of RGMa on endothelial cell (EC) proliferation, migration, tube formation, and cytoskeleton reassembly...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28994298/human-aortic-endothelial-cells-respond-to-shear-flow-in-well-plate-microfluidic-devices
#8
Om Makwana, Hannah Flockton, Gary P Watters, Rizwan Nisar, Gina A Smith, Wanda Fields, Betsy Bombick
Although chronic progressive cardiovascular diseases such as atherosclerosis are often challenging to fully model in vitro, it has been shown that certain in vitro methods can effectively evaluate some aspects of disease progression. This has been demonstrated in in vitro and in vivo studies of endothelial cells that have illustrated the effects of nitric oxide (NO) production, filamentous actin (F-actin) formation, and cell and actin angle alignment on vascular function and homeostasis. Systems utilising shear flow have been established, in order to create a physiologically relevant environment for cells that require shear flow for homeostasis...
September 2017: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/28994005/characterisation-of-human-induced-pluripotent-stem-cell-derived-endothelial-cells-under-shear-stress-using-an-easy-to-use-microfluidic-cell-culture-system
#9
Rsituko Ohtani-Kaneko, Kenjiro Sato, Atsuhiro Tsutiya, Yuka Nakagawa, Kazutoshi Hashizume, Hidekatsu Tazawa
Induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) can contribute to elucidating the pathogenesis of heart and vascular diseases and developing their treatments. Their precise characteristics in fluid flow however remain unclear. Therefore, the aim of the present study is to characterise these features. We cultured three types of ECs in a microfluidic culture system: commercially available human iPS-ECs, human umbilical vein endothelial cells (HUVECs) and human umbilical artery endothelial cells (HUAECs)...
October 9, 2017: Biomedical Microdevices
https://www.readbyqxmd.com/read/28993724/plpp-cin-regulates-seizure-activity-by-the-differential-modulation-of-calsenilin-binding-to-glun1-and-kv4-2-in-mice
#10
Ji-Eun Kim, Hye-Won Hyun, Su-Ji Min, Duk-Shin Lee, A Ran Jeon, Min Ju Kim, Tae-Cheon Kang
Calsenilin (CSEN) binds to Kv4.2 (an A-type K(+) channel) as well as N-methyl-D-aspartate receptor (NMDAR), and modulates their activities. However, the regulatory mechanisms for CSEN-binding to Kv4.2 or NMDAR remain elusive. Here, we demonstrate the novel role of pyridoxal-5'-phosphate phosphatase/chronophin (PLPP/CIN), one of the cofilin-mediated F-actin regulators, in the CSEN binding to Kv4.2 or GluN1 (an NMDAR subunit). PLPP/CIN dephosphorylated CSEN in competition with casein kinase 1, independent of cofilin dephosphorylation...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28993503/myh9-e1841k-mutation-augments-proteinuria-and-podocyte-injury-and-migration
#11
Sylvia Cechova, Fan Dong, Fang Chan, Michael J Kelley, Phillip Ruiz, Thu H Le
Intronic variants of the MYH9 gene that encodes the nonmuscle myosin heavy chain IIA are associated with diabetic nephropathy in European Americans and with sickle cell disease-associated nephropathy. However, the causal functional variants of MYH9 have remained elusive. Rare missense mutations in MYH9 cause macrothrombocytopenia and are occasionally associated with development of nephropathy. The E1841K mutation is among the common MYH9 missense mutations and has been associated with nephropathy in some carriers...
October 9, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/28989119/effects-of-protein-coated-nanofibers-on-conformation-of-gingival-fibroblast-spheroids-potential-utility-for-connective-tissues-regeneration
#12
Gili Kaufman, Ryan Whitescarver, Laiz Nunes, Xavier-Lewis Palmer, Drago Skrtic, Wojtek Tutak
Deep wounds in the gingiva caused by trauma or surgery require a rapid and robust healing of connective tissues. We propose utilizing gas-brushed nanofibers coated with collagen and fibrin for that purpose. Our hypotheses are that protein-coated nanofibers will: (i) attract and mobilize cells in various spatial orientations, and (ii) regulate the expression levels of specific extracellular matrix (ECM)-associated proteins, determining the initial conformational nature of dense and soft connective tissues. Gingival fibroblast monolayers and 3D spheroids were cultured on ECM substrate and covered with gas-blown poly-(DL-lactide-co-glycolide) (PLGA) nanofibers (uncoated/coated with collagen and fibrin)...
October 9, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28976774/proteomic-analysis-of-extracellular-hmgb1-identifies-binding-partners-and-exposes-its-potential-role-in-airway-epithelial-cell-homeostasis
#13
Sharon L I Wong, Joyce To, Jerran Santos, Venkata Sita Rama Raju Allam, John P Dalton, Steven Philip Djordjevic, Sheila Donnelly, Matthew Paul Padula, Maria B Sukkar
The release of damage-associated molecular patterns (DAMPs) by airway epithelial cells is believed to play a crucial role in the initiation and development of chronic airway conditions such as asthma and chronic obstructive pulmonary disease (COPD). Intriguingly, the classic DAMP high-mobility group box-1 (HMGB1) is detected in the culture supernatant of airway epithelial cells under basal conditions, indicating a role for HMGB1 in the regulation of epithelial cellular and immune homeostasis. In order to gain contextual insight into the potential role of HMGB1 in airway epithelial cell homeostasis, we used the orthogonal and complimentary methods of high resolution clear native electrophoresis, immunoprecipitation and pull-downs, coupled to liquid chromatography-tandem mass spectrometry (LC-MS/MS) to profile HMGB1 and its binding partners in the culture supernatant of unstimulated airway epithelial cells...
October 4, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28974722/succinate-promotes-stem-cell-migration-through-the-gpr91-dependent-regulation-of-drp1-mediated-mitochondrial-fission
#14
So Hee Ko, Gee Euhn Choi, Ji Young Oh, Hyun Jik Lee, Jun Sung Kim, Chang Woo Chae, Diana Choi, Ho Jae Han
The role of metabolites produced from stem cell metabolism has been emerged as signaling molecules to regulate stem cell behaviors such as migration. The mitochondrial morphology is closely associated with the metabolic balance and stem cell function. However, the physiological role of succinate on human mesenchymal stem cell (hMSC) migration by regulating the mitochondrial morphology remains unclear. Here, we investigate the effect of succinate on hMSC migration via regulation of mitochondrial dynamics and its related signaling pathway...
October 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28973902/drk-mediated-signaling-to-rho-kinase-is-required-for-anesthesia-resistant-memory-in-drosophila
#15
Vasileia Kotoula, Anastasios Moressis, Ourania Semelidou, Efthimios M C Skoulakis
Anesthesia-resistant memory (ARM) was described decades ago, but the mechanisms that underlie this protein synthesis-independent form of consolidated memory in Drosophila remain poorly understood. Whether the several signaling molecules, receptors, and synaptic proteins currently implicated in ARM operate in one or more pathways and how they function in the process remain unclear. We present evidence that Drk, the Drosophila ortholog of the adaptor protein Grb2, is essential for ARM within adult mushroom body neurons...
September 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28972183/the-actin-related-p41arc-subunit-contributes-to-p21-activated-kinase-1-pak1-mediated-glucose-uptake-into-skeletal-muscle-cells
#16
Ragadeepthi Tunduguru, Jing Zhang, Arianne Aslamy, Vishal A Salunkhe, Joseph T Brozinick, Jeffrey S Elmendorf, Debbie C Thurmond
Defects in translocation of the glucose transporter GLUT4 are associated with peripheral insulin resistance, pre-clinical diabetes, and progression to type 2 diabetes. GLUT4 recruitment to the plasma membrane of skeletal muscle cells requires filamentous (F)-actin remodeling. Insulin signaling in muscle requires p21-activated kinase-1 (PAK1), whose downstream signaling triggers actin remodeling that promotes GLUT4 vesicle translocation and glucose uptake into skeletal muscle cells. Actin remodeling is a cyclic process, and while PAK1 is known to initiate changes to the cortical actin-binding protein cofilin to stimulate the depolymerizing arm of the cycle, how PAK1 might trigger the polymerizing arm of the cycle remains unresolved...
September 25, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28972134/live-imaging-reveals-distinct-modes-of-neutrophil-and-macrophage-migration-within-interstitial-tissues
#17
Francisco Barros-Becker, Pui-Ying Lam, Robert Fisher, Anna Huttenlocher
Cell motility is required for diverse processes during immunity and inflammation. Classically, leukocyte motility is defined as an amoeboid type of migration, however some leukocytes, like macrophages, also employ a more mesenchymal mode. Here, we sought to characterize the mechanisms that regulate neutrophil and macrophage migration in vivo using dual real time imaging of leukocyte motility within interstitial tissues in zebrafish larvae. Neutrophils displayed a rounded morphology and rapid protease independent motility, lacked defined paxillin punctae and had persistent rearward polarization of stable F-actin and the microtubule network...
September 28, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28972078/f-actin-meditated-focusing-of-vesicles-at-the-cell-tip-is-essential-for-polarized-growth
#18
Jeffrey P Bibeau, James L Kingsley, Fabienne Furt, Erkan Tuzel, Luis Vidali
Filamentous actin has been shown to be essential for tip growth in an array of plant models, including Physcomitrella patens. One hypothesis is that diffusion can transport secretory vesicles, while actin plays a regulatory role during secretion. Alternatively, it is possible that actin-based transport is necessary to overcome vesicle transport limitations to sustain secretion. Therefore, a quantitative analysis of diffusion, secretion kinetics and geometry is necessary to clarify the role of actin in polarized growth...
October 2, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28967086/intestinal-mucosal-barrier-dysfunction-in-sap-patients-with-mods-ameliorated-by-continuous-blood-purification
#19
Qing Shen, Zhengrong Li, Shanshan Huang, Liman Li, Hua Gan, Xiao-Gang Du
BACKGROUND: Dysfunction of the intestinal mucosal barrier plays an important role in the pathophysiology of severe acute pancreatitis (SAP). Continuous blood purification (CBP) has been shown to improve the prognosis of SAP patients. In order to investigate the effect of CBP on intestinal mucosal barrier dysfunction in SAP patients with MODS, we conducted in vivo and in vitro experiments to explore the underlying mechanisms. METHODS: The markers for the assessment of intestinal mucosal barrier function including serum diamine oxidase (DAO), endotoxin and intestinal epithelial monolayer permeability were detected during CBP therapy...
September 14, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28962923/vesicular-movements-in-the-growth-cone
#20
REVIEW
Motohiro Nozumi, Michihiro Igarashi
Growth cones, which are the highly motile tips of extending neuronal processes in developing neurons, have many vesicles. These vesicles are likely essential for the membrane expansion that is required for nerve growth, and probably coordinate with rearrangement of the cytoskeletons. Such mechanisms are poorly understood from molecular and cell biological aspects. Recently, we used superresolution microscopic approaches and described new mechanisms that are involved in the interaction between the vesicles and F-actin in the leading edge of the peripheral domain...
September 26, 2017: Neurochemistry International
keyword
keyword
14400
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"