keyword
MENU ▼
Read by QxMD icon Read
search

F-actin

keyword
https://www.readbyqxmd.com/read/29222746/cadmium-favors-f-actin-depolymerization-in-rat-renal-mesangial-cells-by-site-specific-disulfide-based-dimerization-of-the-cap1-protein
#1
Ying Liu, Weiqun Xiao, Manasi Shinde, Jeffrey Field, Douglas M Templeton
Cadmium is a toxic metal that produces oxidative stress and has been shown to disrupt the actin cytoskeleton in rat renal mesangial cells (RMC). In a survey of proteins that might undergo Cd2+-dependent disulfide crosslinking, we identified the adenylyl cyclase-associated protein, CAP1, as undergoing a dimerization in response to Cd2+ (5-40 µM) that was sensitive to disulfide reducing agents, was reproduced by the disulfide crosslinking agent diamide, and was shown by site-directed mutagenesis to involve the Cys29 residue of the protein...
December 8, 2017: Archives of Toxicology
https://www.readbyqxmd.com/read/29222169/nanoscale-tuning-of-vcam-1-determines-vla-4-dependent-melanoma-cell-plasticity-on-rgd-motifs
#2
Katharina Amschler, Eugen Kossmann, Luise Erpenbeck, Sebastian Kruss, Tillmann Schill, Margarete Schön, Sigrid M C Möckel, Joachim P Spatz, Michael P Schön
The biophysical fine-tuning of cancer cell plasticity is crucial for tumor progression but remains largely enigmatic. Although Vascular Cell Adhesion Molecule-1 (VCAM-1/CD106) has been implicated in melanoma progression, here its presentation on endothelial cells was associated with diminished melanoma cell spreading. Using a specific nanoscale-modulation of VCAM-1 (tunable from 70 to 670 ligands/µm²) next to integrin ligands (RGD motifs) in a bifunctional system, reciprocal regulation of integrin α4 (ITGA4/VLA-4/CD49d)-dependent adhesion and spreading of melanoma cells was found...
December 8, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29220720/induction-of-thymic-stromal-lymphopoietin-by-a-steroid-alkaloid-derivative-in-mouse-keratinocytes
#3
Yan Weng, Natsumi Mizuno, Jiangxu Dong, Ryosuke Segawa, Takayuki Yonezawa, Byung Yoon Cha, Je-Tae Woo, Takahiro Moriya, Masahiro Hiratsuka, Noriyasu Hirasawa
Thymic stromal lymphopoietin (TSLP) plays critical roles in inducing and exacerbating allergic diseases. Chemical compounds that induce TSLP production can enhance sensitization to antigens and exacerbate allergic inflammation. Hence, identifying such chemicals will be important to prevent an increase in allergic diseases. In the present study, we found, for the first time, that a steroid alkaloid derivative, code no. 02F04, concentration and time dependently induced mRNA expression and production of TSLP in a mouse keratinocyte cell line, PAM212...
December 5, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29218606/pi3k-is-a-linker-between-l-selectin-and-psgl-1-signaling-to-il-18-transcriptional-activation-at-the-promoter-level
#4
Jixian Luo
L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are adhesion molecules which induce similar physiological events. Our previous paper showed that phosphatidylinositol 3-kinase (PI3K) played a crucial role in L-selectin- and PSGL-1-mediated F-actin redistribution and assembly during neutrophil rolling on E-selectin. However, it is not clear whether L-selectin and PSGL-1 induce other similar physiology events by PI3K. Here, we investigated the possibility of PI3K linking the signaling pathways of L-selectin and PSGL-1 to IL-18 transcription...
December 7, 2017: Inflammation
https://www.readbyqxmd.com/read/29217089/fully-coupled-mathematical-modeling-of-actomyosin-cytosolic-two-phase-flow-in-a-highly-deformable-moving-keratocyte-cell
#5
M R Nikmaneshi, B Firoozabadi, M S Saidi
Interaction between intracellular dynamics and extracellular matrix (ECM) generally occurred into very thin fragment of moving cell, namely lamellipodia, enables all movable cells to crawl on ECM. In fast-moving cells such as fish Keratocytes, Lamellipodia including most cell area finds a fan-like shape during migration, with a variety of aspect ratio function of fish type. In this work, our purpose is to present a novel and more complete two-dimensional continuum mathematical model of actomyosin-cytosolic two-phase flow of a self-deforming Keratocyte with circular spreaded to steady fan-like shape...
December 2, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29216499/vitamin-d3-decreases-glycolysis-and-invasiveness-and-increases-cellular-stiffness-in-breast-cancer-cells
#6
Julianna Maria Santos, Zeina Shereen Khan, Maliha Tabassum Munir, Kaiser Tarafdar, Shaikh Mizanoor Rahman, Fazle Hussain
Breast cancer is one of the major causes of death in the USA. Cancer cells, including breast, have high glycolysis rates to meet their energy demands for survival and growth. Vitamin D3 (VD3) is important for many important physiological processes such as bone mineralization, but its anticancer role is yet to be proven. We find that VD3 treatment significantly down-regulates glycolytic enzymes and genes and decreases glucose uptake - for both lowly metastatic MCF-7 and highly metastatic MDA-MB-231 (MB231) breast cancer cells...
November 2, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29215055/impairing-the-function-of-mlck-myosin-va-or-myosin-vb-disrupts-rhinovirus-b14-replication
#7
Antonio Real-Hohn, D William Provance, Rafael Braga Gonçalves, Caio Bidueira Denani, Andréa Cheble de Oliveira, Verônica P Salerno, Andre Marco Oliveira Gomes
Together, the three human rhinovirus (RV) species are the most frequent cause of the common cold. Because of their high similarity with other viral species of the genus Enterovirus, within the large family Picornaviridae, studies on RV infectious activities often offer a less pathogenic model for more aggressive enteroviruses, e.g. poliovirus or EV71. Picornaviruses enter via receptor mediated endocytosis and replicate in the cytosol. Most of them depend on functional F-actin, Rab proteins, and probably motor proteins...
December 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29213129/deep-tissue-analysis-of-distal-aqueous-drainage-structures-and-contractile-features
#8
Jose M Gonzalez, Minhee K Ko, Young-Kwon Hong, Robert Weigert, James C H Tan
Outflow resistance in the aqueous drainage tract distal to trabecular meshwork is potentially an important determinant of intraocular pressure and success of trabecular bypass glaucoma surgeries. It is unclear how distal resistance is modulated. We sought to establish: (a) multimodal 2-photon deep tissue imaging and 3-dimensional analysis of the distal aqueous drainage tract (DT) in transgenic mice in vivo and ex vivo; (b) criteria for distinguishing the DT from blood and lymphatic vessels; and (c) presence of a DT wall organization capable of contractility...
December 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29211998/tropomyosin-must-interact-weakly-with-actin-to-effectively-regulate-thin-filament-function
#9
Michael J Rynkiewicz, Thavanareth Prum, Stephen Hollenberg, Farooq A Kiani, Patricia M Fagnant, Steven B Marston, Kathleen M Trybus, Stefan Fischer, Jeffrey R Moore, William Lehman
Elongated tropomyosin, associated with actin-subunits along the surface of thin filaments, makes electrostatic interactions with clusters of conserved residues, K326, K328, and R147, on actin. The association is weak, permitting low-energy cost regulatory movement of tropomyosin across the filament during muscle activation. Interestingly, acidic D292 on actin, also evolutionarily conserved, lies adjacent to the three-residue cluster of basic amino acids and thus may moderate the combined local positive charge, diminishing tropomyosin-actin interaction and facilitating regulatory-switching...
December 5, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/29210641/dynamic-localization-of-hepatocellular-transporters-role-in-biliary-excretion-and-impairment-in-cholestasis
#10
Marcelo Gabriel Roma, Ismael R Barosso, Gisel S Miszczuk, Fernando A Crocenzi, Enrique J Sanchez Pozzi
Bile flow generation is driven by the vectorial transfer of osmotically active compounds from sinusoidal blood into a confined space, the bile canaliculus. Hence, localization of hepatocellular transporters relevant to bile formation is crucial for bile secretion. Hepatocellular transporters are localized either in the plasma membrane or in recycling endosomes, from where they can be relocated to the plasma membrane on demand, or endocytosed when the demand decreases. The balance between endocytic internalization/exocytic targeting to/from this recycling compartment is therefore the main determinant of the hepatic capability to generate bile, and to dispose endo- and xenobiotics...
December 5, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29209287/erm-proteins-play-distinct-roles-in-cell-invasion-by-extracellular-amastigotes-of-trypanosoma-cruzi
#11
Éden R Ferreira, Alexis Bonfim-Melo, Esteban M Cordero, Renato A Mortara
The protozoan parasite Trypanosoma cruzi is the causative agent of Chagas' disease. In mammalian hosts, T. cruzi alternates between trypomastigote and amastigote forms. Additionally, trypomastigotes can differentiate into amastigotes in the extracellular environment generating infective extracellular amastigotes (EAs). Ezrin-radixin-moesin (ERM) are key proteins linking plasma membrane to actin filaments, the major host cell component responsible for EA internalization. Our results revealed that depletion of host ezrin and radixin but not moesin inhibited EAs invasion in HeLa cells...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29208528/heterochromatin-protein-hp-1%C3%AE-is-not-only-in-the-nucleus-but-also-in-the-cytoplasm-interacting-with-actin-in-both-cell-compartments
#12
Nancy L Charó, Natalia M Galigniana, Graciela Piwien-Pilipuk
Confocal and electron microscopy images, and WB analysis of cellular fractions revealed that HP1γ is in the nucleus but also in the cytoplasm of C2C12 myoblasts, myotubes, skeletal and cardiac muscles, N2a, HeLa and HEK293T cells. Signal specificity was tested with different antibodies and by HP1γ knockdown. Leptomycin B treatment of myoblasts increased nuclear HP1γ, suggesting that its nuclear export is Crm-1-dependent. HP1γ exhibited a filamentous pattern of staining partially co-localizing with actin in the cytoplasm of myotubes and myofibrils...
December 2, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29199952/cryo-em-structures-reveal-specialization-at-the-myosin-vi-actin-interface-and-a-mechanism-of-force-sensitivity
#13
Pinar S Gurel, Laura Y Kim, Paul V Ruijgrok, Tosan Omabegho, Zev Bryant, Gregory M Alushin
Despite extensive scrutiny of the myosin superfamily, the lack of high-resolution structures of actin-bound states has prevented a complete description of its mechanochemical cycle and limited insight into how sequence and structural diversification of the motor domain gives rise to specialized functional properties. Here we present cryo-EM structures of the unique minus-end directed myosin VI motor domain in rigor (4.6 Å) and Mg-ADP (5.5 Å) states bound to F-actin. Comparison to the myosin IIC-F-actin rigor complex reveals an almost complete lack of conservation of residues at the actin-myosin interface despite preservation of the primary sequence regions composing it, suggesting an evolutionary path for motor specialization...
December 4, 2017: ELife
https://www.readbyqxmd.com/read/29199880/exposure-to-cigarette-smoke-extract-and-lipopolysaccharide-modifies-cytoskeleton-organization-in-bronchial-epithelial-cells
#14
Claudia D'Anna, Diego Cigna, Caterina Di Sano, Serena Di Vincenzo, Paola Dino, Maria Ferraro, Luca Bini, Laura Bianchi, Francesca Di Gaudio, Mark Gjomarkaj, Elisabetta Pace
The integrity of the respiratory epithelium is crucial for airway homeostasis. Tobacco smoke exposure and recurrent infections of the airways play a crucial role in the progression and in the decline of the respiratory function in chronic obstructive pulmonary disease (COPD). The aim of this study was to detect differentially expressed proteins in a bronchial epithelial cell line (16-HBE) stimulated with cigarette smoke extract (CSE) and lipopolysaccharide (LPS), a constituent of gram-negative bacteria, alone and/or in combination, by using two-dimensional electrophoresis (2DE) analysis coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
December 4, 2017: Experimental Lung Research
https://www.readbyqxmd.com/read/29196604/camkii-regulates-the-de-palmitoylation-and-synaptic-removal-of-akap79-150-to-mediate-structural-ltd
#15
Kevin M Woolfrey, Heather O'Leary, Dayton J Goodell, Holly R Robertson, Eric A Horne, Steven J Coultrap, Mark L Dell'Acqua, K Ulrich Bayer
Both long-term potentiation (LTP) and depression (LTD) of excitatory synapse strength require the Ca2+/calmodulin (CaM)-dependent protein kinase II (CaMKII) and its autonomous activity generated by T286 autophosphorylation. Additionally, LTP and LTD are correlated with dendritic spine enlargement and shrinkage that are accompanied by the synaptic accumulation or removal, respectively, of the AMPA-receptor regulatory scaffold protein A-kinase anchoring protein (AKAP) 79/150. We show here that the spine shrinkage associated with LTD indeed requires synaptic AKAP79/150 removal, which in turn requires CaMKII activity...
December 1, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29195976/the-effect-of-cacl2-marination-on-the-tenderizing-pathway-of-goose-meat-during-conditioning
#16
Xuan Li, Yangying Sun, Daodong Pan, Ying Wang, Jinxuan Cao
In order to figure out the effect of CaCl2 on the tenderizing pathway of goose meat, breast muscles of thirty-two Eastern Zhejiang White Geese were divided into three treatments: the control, 150 and 300mM CaCl2. Shear force, myofibrillar fraction index (MFI), actin filaments and F-actin, G-actin and tropomodulins (Tmods) levels were investigated during 168h. Results showed that 300mM treatment had lower shear force at 48, 96 and 168h and higher MFI at 24, 48, 96 and 168h than the control. The rate of actin filaments disruption, the decrease of F-actin, the degradation of Tmods, the increase of G-actin in 300mM treatment was faster than 150mM treatment; the rate in the control was the slowest among treatments...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29192233/kaempferide-prevents-titanium-particle-induced-osteolysis-by-suppressing-jnk-activation-during-osteoclast-formation
#17
Zixian Jiao, Weifeng Xu, Jisi Zheng, Pei Shen, An Qin, Shanyong Zhang, Chi Yang
Kaempferide (KF) is an O-methylated flavonol, a natural plant extract, which is often found in Kaempferia galanga. It has a variety of effects including anti-carcinogenic, anti-inflammatory, anti-oxidant, anti-bacterial and anti-viral properties. In this study, we aimed to investigate whether KF effectively inhibits titanium particle induced calvarial bone loss via down regulation of the JNK signaling pathway. In the mice with titanium particle induced calvarial osteolysis, the Low dose of KF mildly reduced the resorption pits while in the high dose group, fewer scattered pits were observed on the surface of calvarium...
November 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29191847/wasp-family-proteins-and-formins-compete-in-pseudopod-and-bleb-based-migration
#18
Andrew J Davidson, Clelia Amato, Peter A Thomason, Robert H Insall
Actin pseudopods induced by SCAR/WAVE drive normal migration and chemotaxis in eukaryotic cells. Cells can also migrate using blebs, in which the edge is driven forward by hydrostatic pressure instead of actin. In Dictyostelium discoideum, loss of SCAR is compensated by WASP moving to the leading edge to generate morphologically normal pseudopods. Here we use an inducible double knockout to show that cells lacking both SCAR and WASP are unable to grow, make pseudopods or, unexpectedly, migrate using blebs. Remarkably, amounts and dynamics of actin polymerization are normal...
November 30, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29191834/three-mammalian-tropomyosin-isoforms-have-different-regulatory-effects-on-nonmuscle-myosin-2b-and-filamentous-beta-actin-in-vitro
#19
Salma Pathan-Chhatbar, Manuel H Taft, Theresia Reindl, Nikolas Hundt, Sharissa L Latham, Dietmar J Manstein
The metazoan actin cytoskeleton supports a wide range of contractile and transport processes. Recent studies have shown how the dynamic association with specific tropomyosin isoforms generates actin filament populations with distinct functional properties. However, critical details of the associated molecular interactions remain unclear. Here, we report the properties of actomyosin-tropomyosin complexes containing filamentous β-actin, nonmuscle myosin-2B (NM-2B) constructs and either tropomyosin isoform Tpm1...
November 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29188873/p49-strap-a-serum-response-factor-binding-protein-srfbp1-is-involved-in-the-redistribution-of-cytoskeletal-f-actin-proteins-during-glucose-deprivation
#20
E D Williams, S C Rogers, X Zhang, G Azhar, J Y Wei
The functional decline that usually accompanies adult aging also encompasses cellular changes including cytoplasmic architecture. In addition to their role in cytoskeletal structure, actin microfilaments have important roles in various cellular processes, including cell-to-cell communication and intracellular signaling. Age-related diseases and late-stage cellular morphological appearances often correlate with altered f-actin structure, which has been observed most notably in cancer. What remains less clear are the molecular pathways that may be involved in normal and premature aging-induced f-actin changes...
2017: Journal of Nutrition, Health & Aging
keyword
keyword
14400
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"