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Cell migration

Hongyan Lin, Xiaohong Zhang, Nannan Feng, Ruoyang Wang, Weituo Zhang, Xiaobei Deng, Yu Wang, Xiao Yu, Xiaofei Ye, Lei Li, Ying Qian, Herbert Yu, Biyun Qian
BACKGROUND/AIMS: Ecological studies have shown that air pollution and prevalence of cigarette smoking are positively correlated. Evidence also suggests a synergistic effect of cigarette smoking and PM2.5 exposure (Environmental Particulate Matter ≤ 2.5 µm in diameter) on lung cancer risk. We aimed to evaluate the interaction between smoking prevalence and PM2.5 pollution in relation to lung cancer mortality and determine its underlying mechanisms in vitro. METHODS: "MOVER" method was used to analyze the interaction between smoking prevalence and PM2...
June 15, 2018: Cellular Physiology and Biochemistry
Meriem Echbarthi, Josefine Vallin, Julie Grantham
Chaperonin containing tailless complex polypeptide 1 (CCT) is a molecular chaperone consisting of eight distinct protein subunits, that when oligomeric is essential for the folding of newly synthesized tubulin and actin. In addition to folding, CCT activity includes functions of individual subunits in their monomeric form. For example, when CCTδ monomer levels are increased in cultured mammalian cells, numerous cell surface protrusions are formed from retraction fibres, indicating that an underlying function for the CCTδ monomer exists...
June 15, 2018: Experimental Cell Research
Li Shen, Qing Ke, Jingjing Chai, Chuanyi Zhang, Li Qiu, Feng Peng, Xinzhou Deng, Zhiguo Luo
Phosphoprotein associated with glycosphingolipid-enriched microdomains 1(PAG1) is a ubiquitous protein that is essential for the development and progression of various malignancies. A previous study in our laboratory confirmed that PAG1 plays an important role in modulating the inherent radioresistance of laryngeal cancer cells, but the underlying mechanisms are still poorly defined. In this study, we found that PAG1 was significantly increased in laryngeal cancer tissues compared to adjacent non-tumor tissues (P < 0...
June 15, 2018: Experimental Cell Research
Mayrim Machado Gomes Smaul, Alexandre Budu, Georgina Nuri Montagna, Taíse Fernanda da Silva Ferrara, Sarah El Chamy Maluf, Piero Bagnaresi, Marcelo Marcondes Ferreira Machado, Fellipe Bronze Dos Santos, Mauro Ferreira de Azevedo, Adriana Karaoglanovic Carmona, Marcos Leoni Gazarini
Calcium signaling has an essential role in fundamental processes of Plasmodium life cycle, including migration, cell invasion and parasite development. Two important players in calcium homeostasis, the Histidine Triad (HIT) protein that is implicated in calcium signaling in mammalian cells and calmodulin, which is a classic calcium sensor in eukaryotes are present in Plasmodium falciparum, however theirs function is unknown in the parasite. Here, we investigated the involvement of the P. falciparum Histidine Triad protein (PfHint-1) and calmodulin (PfCaM) in calcium signaling and intracellular proteolysis...
June 15, 2018: Biochemical and Biophysical Research Communications
Ying Liu, Hongyue Zhang, Lixin Yan, Wei Du, Min Zhang, He Chen, Lixin Zhang, Guangqun Li, Jijin Li, Yinchu Dong, Daling Zhu
Matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) are the predominant gelatinases in the developing lung. Studies have shown that the expression of MMP-2 and MMP-9 is upregulated in hypoxic fibroblasts, 15-hydroxyeicosatetraenoic acid (15-HETE) regulated fibroblasts migration via modulating MMP-2 or MMP-9, and that hypoxia/15-HETE is a predominant contributor to the development of pulmonary arterial hypertension (PAH) through increased angiogenesis. However, the roles of MMP-2 and MMP-9 in pulmonary arterial endothelial cells (PAECs) angiogenesis as well as the molecular mechanism of hypoxia-regulated MMP-2 and MMP-9 expression have not been identified...
June 15, 2018: Journal of Molecular and Cellular Cardiology
Steven C Eastlack, Shengli Dong, Yin Y Mo, Suresh K Alahari
Malat1 is a long noncoding RNA with a wide array of functions, including roles in regulating cancer cell migration and metastasis. However, the nature of its involvement in control of these oncogenic processes is incompletely understood. In the present study, we investigate the role of Malat1 and the effects of Malat1 KO in a breast cancer cell model. Our selection of Malat1 as the subject of inquiry followed initial screening experiments seeking to identify lncRNAs which are altered in the presence or absence of Nischarin, a gene of interest previously discovered by our lab...
2018: PloS One
Bei Zhang, Zhenghui Deng, Baishuang Zeng, Shilong Yang, Xin Chen, Xueqing Xu, Jiguo Wu
Voltage-gated sodium channel activity enhances the motility and oncogene expression of metastasic cancer cells that express a neonatal alternatively spliced form of the NaV1.5 isoform. We reported previously that FS50, a salivary protein from Xenopsylla cheopis, showed inhibitory activity against the NaV1.5 channel when assayed in HEK 293T cells and antiarrhythmia effects on rats and monkeys after induction of arrhythmia by BaCl2. This study aims to identify the effect of FS50 on voltage-gated sodium channel activity and the motility of MDA-MB-231 human breast cancer cells in vitro...
June 16, 2018: Anti-cancer Drugs
Zeqi Zheng, Lijuan Liu, Yuliang Zhan, Songping Yu, Ting Kang
OBJECTIVE: To explore the potential mechanism of miRNA released from adipose-derived stem cell (ADSC)-derived micro vesicle (MV) on the modulation of proliferation, migration and invasion of endothelial cells. MATERIALS AND METHODS: miR-210 level was detected by qT-PCR. Alix, VEGF and RUNX3 expressions were detected by Western blot. The proliferation, migration and invasion of human umbilical vein endothelial cells (HUVECs) were observed by MTT assay and Transwell assay...
June 18, 2018: Cell Cycle
Judit Symmank, Cathrin Bayer, Christiane Schmidt, Anne Hahn, Daniel Pensold, Geraldine Zimmer
Epigenetic mechanisms of gene regulation, including DNA methylation and histone modifications, call increasing attention in the context of development and human health. Thereby, interactions between DNA methylating enzymes and histone modifications tremendously multiply the spectrum of potential regulatory functions. Epigenetic networks are critically involved in the establishment and functionality of neuronal circuits that are composed of gamma-aminobutyric acid (GABA)-positive inhibitory interneurons and excitatory principal neurons in the cerebral cortex...
June 18, 2018: Epigenetics: Official Journal of the DNA Methylation Society
Jian Chen, Qian Wang, Wei Zhou, Zheng Zhou, Peng-Yu Tang, Tao Xu, Wei Liu, Lin-Wei Li, Lin Cheng, Zhi-Min Zhou, Jin Fan, Guo-Yong Yin
GPCR kinase 2-interacting protein-1 (GIT1) is a scaffold protein that plays an important role in cell adaptation, proliferation, migration, and differentiation; however, the role of GIT1 in the regulation of neuronal death after spinal cord injury remains obscure. Here, we demonstrate that GIT1 deficiency remarkably increased neuronal apoptosis and enhanced JNK/p38 signaling, which resulted in stronger motor deficits by ischemia-reperfusion in vivo, consistent with the finding of oxygen-glucose deprivation/reoxygenation-induced neuronal injury in vitro...
June 18, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Yue Li, Xiaoyan Wang, Xishuang Wang, Lu Wan, Yanping Liu, Yongyu Shi, Lining Zhang, Zhenghui Fang, Zengtao Wei
Endometriosis (EM) is a kind of estrogen-dependent disease in reproductive-age women. Ovarian endometriosis is the most common type. Although endometriosis is a benign disease, it shares many similar features with cancers. Programmed cell death 4 (PDCD4), a newly identified tumor suppressor, plays an important role in inhibiting tumorigenesis and tumor progression at the transcriptional and translational levels. To explore the roles of PDCD4 in endometriosis, we detected the expression of PDCD4 in control endometrium and eutopic/ectopic endometrium of ovarian endometriosis patients, and analyzed the effects of PDCD4 on the biological behaviors of endometrial cell lines and primary endometrial cells...
June 15, 2018: Biology of Reproduction
Maida Vartanian, Pilar de la Cruz, Subhayan Biswas, Ganesh D Sharma, Fernando Langa
In an effort to improve the short-circuit current and fill factor, organic solar cells have been developed with ternary blending in a single bulk heterojunction active layer. We report here several all small molecule organic solar cells based on ternary bulk heterojunction active layers. These layers consist of two small molecule porphyrin donors (MV71 and MV72), which have the same backbone but different end-capping acceptor units, and PC71BM as the acceptor. The organic solar cells showed overall power conversion efficiencies of 3...
June 18, 2018: Nanoscale
Nadine Santana-Magal, Diana Rasoulouniriana, Corey Saperia, Amit Gutwillig, Peleg Rider, Edgar G Engleman, Yaron Carmi
Dendritic cells (DC) are heterogeneous cell populations that differ in their cell membrane markers, migration patterns and distribution, and in their antigen presentation and T cell activation capacities. Since most vaccinations of experimental tumor models require millions of DC, they are widely isolated from the bone marrow or spleen. However, these DC significantly differ from blood and tumor DC in their responses to immune complexes (IC), and presumably to other Syk-coupled lectin receptors. Importantly, given the sensitivity of DC to danger-associated molecules, the presence of endotoxins or antibodies that crosslink activation receptors in one of the isolating steps could result in the priming of DC and thus affect the parameters, or at least the dosage, required to activate them...
May 31, 2018: Journal of Visualized Experiments: JoVE
Tzu-Yu Huang, Yi-Hsin Lin, Heng-Ai Chang, Tzu-Ying Yeh, Ya-Han Chang, Yi-Fan Chen, Ying-Chi Chen, Chun-Chun Li, Wen-Tai Chiu
Platelet-derived growth factor (PDGF) has mitogenic and chemotactic effects on fibroblasts. An increase in intracellular Ca2+ is one of the first events that occurs following the stimulation of PDGF receptors (PDGFRs). PDGF activates Ca2+ elevation by activating the phospholipase C gamma (PLC&gamma;)-signaling pathway, resulting in ER Ca2+ release. Store-operated Ca2+ entry (SOCE) is the major form of extracellular Ca2+ influx following depletion of ER Ca2+ stores and stromal interaction molecule 1 (STIM1) is a key molecule in the regulation of SOCE...
June 18, 2018: International Journal of Molecular Sciences
Francesca Luongo, Giuseppe Pietropaolo, Mathieu Gautier, Isabelle Dhennin-Duthille, Halima Ouadid-Ahidouch, Federica I Wolf, Valentina Trapani
Intestinal magnesium (Mg) uptake is essential for systemic Mg homeostasis. Colon cells express the two highly homologous transient receptor potential melastatin type (TRPM) 6 and 7 Mg2+ channels, but their precise function and the consequences of their mutual interaction are not clear. To explore the functional role of TRPM6 and TRPM7 in the colon, we used human colon cell lines that innately express both channels and analyzed the functional consequences of genetic knocking-down, by RNA interference, or pharmacological inhibition, by NS8593, of either channel...
June 18, 2018: Nutrients
Li Fan, Yichao Lu, Xiulian Shen, Hong Shao, Lun Suo, Qiang Wu
Diverse clustered protocadherins are thought to function in neurite morphogenesis and neuronal connectivity in the brain. Here we report that the protocadherin alpha ( Pcdha ) gene cluster regulates neuronal migration during cortical development and cytoskeletal dynamics in primary cortical culture through the WAVE (Wiskott-Aldrich syndrome family verprolin homologous protein, also known as WASP or Wasf) complex. In addition, overexpression of proline-rich tyrosine kinase 2 (Pyk2, also known as Ptk2b, Cakb, Raftk, Fak2, and Cadtk), a non-receptor cell-adhesion kinase and scaffold protein downstream of Pcdha, impairs cortical neuron migration via inactivation of the small GTPase Rac1...
June 18, 2018: ELife
Kyle Crassini, Tahni Pyke, Yandong Shen, William S Stevenson, Richard I Christopherson, Stephen P Mulligan, Oliver Giles Best
The Raf-1 kinase inhibitory protein (RKIP) is an important regulatory element in multiple signaling pathways, including MAPK-ERK1/2. We investigated whether targeted disruption of RKIP is a therapeutic option for chronic lymphocytic leukemia (CLL). The RKIP inhibitor locostatin-induced apoptosis of CLL cells, irrespective of poor prognostic indications or treatment history. Locostatin down-regulated MAPK-ERK1/2 and AKT phosphorylation, decreased expression of the chemokine receptor CXCR4 (p = .04) and reduced the migratory capacity of CLL cells toward stroma-derived factor 1α (SDF-1α, p = ...
June 18, 2018: Leukemia & Lymphoma
Karthik M Kodigepalli, Serena Bonifati, Nagaraja Tirumuru, Li Wu
Sterile alpha motif and HD domain-containing protein 1 (SAMHD1) is a mammalian dNTP hydrolase that acts as a negative regulator in the efficacy of cytarabine treatment against acute myeloid leukemia (AML). However, the role of SAMHD1 in AML development and progression remains unknown. We have reported that SAMHD1 knockout (KO) in the AML-derived THP-1 cells results in enhanced proliferation and reduced apoptosis, but the underlying mechanisms are unclear. Here we show that SAMHD1 KO in THP-1 cells increased PI3K activity and reduced expression of the tumor suppressor PTEN...
June 18, 2018: Cell Cycle
Heonjae Jeong, Edmund G Seebauer, Elif Ertekin
Properties related to transport such as self-diffusion coefficients are relevant to fuel cells, electrolysis cells, and chemical/gas sensors. Prediction of self-diffusion coefficients from first-principles involves precise determination of both enthalpy and entropy contributions for point defect formation and migration. We use first-principles density functional theory to estimate the self-diffusion coefficient for neutral O0i and doubly ionized Oi2- interstitial oxygen in rutile TiO2 and compare the results to prior isotope diffusion experiments...
June 18, 2018: Physical Chemistry Chemical Physics: PCCP
Kamyar Esmaeili Pourfarhangi, Edgar Cardenas De La Hoz, Andrew R Cohen, Bojana Gligorijevic
Cancer cell migration is essential for metastasis, during which cancer cells move through the tumor and reach the blood vessels. In vivo , cancer cells are exposed to contact guidance and chemotactic cues. Depending on the strength of such cues, cells will migrate in a random or directed manner. While similar cues may also stimulate cell proliferation, it is not clear whether cell cycle progression affects migration of cancer cells and whether this effect is different in random versus directed migration. In this study, we tested the effect of cell cycle progression on contact guided migration in 2D and 3D environments, in the breast carcinoma cell line, FUCCI-MDA-MB-231...
September 2018: APL bioengineering
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