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https://www.readbyqxmd.com/read/28728294/-depletion-of-gp73-inhibits-invasion-and-metastasis-of-hepatocellular-carcinoma-cells
#1
L P Liu, J K Chen, Y M Liu, D H Zhang, J Zhang, X L Yang
Objective: To explore the in vitro and in vivo effect of GP73 on the proliferation, invasion and metastasis in hepatocellular carcinoma. Methods: GP73 gene was knocked out using CRISPR/Cas9 gene editing system in H22 and HepG2 cells, and stable knock out strains were constructed. The knockout efficiency was measured by western blot. Colony formation assay was used to detect the effect of GP73 on long-term survival ability. Cells were then highly synchronized in G(1) phase upon treatment with cell synchronization reagents (mimosine), and the percentage of cells in G(2)/M phase at different time points was detected by flow cytometry...
July 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28727830/the-receptor-tyrosine-kinase-axl-promotes-migration-and-invasion-in-colorectal-cancer
#2
Diana J Uribe, Edward K Mandell, Adam Watson, Jesse D Martinez, Jonathan A Leighton, Sourav Ghosh, Carla V Rothlin
The receptor tyrosine kinases (RTKs) TYRO3, AXL and MERTK (TAM) have well-described oncogenic functions in a number of cancers. Notwithstanding, TAM RTKs are also potent and indispensable inhibitors of inflammation. The combined deletion of Axl and Mertk in mice enhances chronic inflammation and autoimmunity, including increased inflammation in the gut and colitis-associated cancer. On the other hand, deletion of Tyro3 increases the risk of allergic responses. Therefore, the indiscriminate inhibition of these TAM RTKs could result in undesirable immunological diseases...
2017: PloS One
https://www.readbyqxmd.com/read/28727754/dysregulation-of-endothelial-colony-forming-cell-function-by-a-negative-feedback-loop-of-circulating-mir-146a-and-146b-in-cardiovascular-disease-patients
#3
Ting-Yu Chang, Wei-Chi Tsai, Tse-Shun Huang, Shu-Han Su, Chih-Young Chang, Hsiu-Yen Ma, Chun-Hsien Wu, Chih-Yung Yang, Chi-Hung Lin, Po-Hsun Huang, Cheng-Chung Cheng, Shu-Meng Cheng, Hsei-Wei Wang
Functional impairment of endothelial colony-forming cells (ECFCs), a specific cell lineage of endothelial progenitor cells (EPCs) is highly associated with the severity of coronary artery disease (CAD), the most common type of cardiovascular disease (CVD). Emerging evidence show that circulating microRNAs (miRNAs) in CAD patients' body fluid hold a great potential as biomarkers. However, our knowledge of the role of circulating miRNA in regulating the function of ECFCs and the progression of CAD is still in its infancy...
2017: PloS One
https://www.readbyqxmd.com/read/28727734/microrna-200c-inhibits-epithelial-mesenchymal-transition-invasion-and-migration-of-lung-cancer-by-targeting-hmgb1
#4
Po-Len Liu, Wei-Lun Liu, Jia-Ming Chang, Yung-Hsiang Chen, Yu-Peng Liu, Hsuan-Fu Kuo, Chong-Chao Hsieh, Yu-Sian Ding, Wei-Wei Chen, Inn-Wen Chong
MicroRNAs (miRs) play critical roles in cancer development, proliferation, epithelial-mesenchymal transition (EMT), invasion, and migration through regulating the expression of oncogenes and tumour suppressor genes. Previous studies have indicated that miR-200c acts as a tumour suppressor in various cancers by downregulating high-mobility group box 1 (HMGB1) and thereby suppressing EMT and metastasis. In addition, miR-200c was reported to be downregulated and correlated with poor outcomes in non-small cell lung cancer (NSCLC)...
2017: PloS One
https://www.readbyqxmd.com/read/28727496/modulation-of-vegf-induced-migration-and-network-formation-by-lymphatic-endothelial-cells-roles-of-platelets-and-podoplanin
#5
Stacey A Langan, Leyre Navarro-Núñez, Steve P Watson, Gerard B Nash
Lymphatic endothelial cells (LEC) express the transmembrane receptor podoplanin whose only known endogenous ligand CLEC-2 is found on platelets. Both podoplanin and CLEC-2 are required for normal lymphangiogenesis as mice lacking either protein develop a blood-lymphatic mixing phenotype. We investigated the roles of podoplanin and its interaction with platelets in migration and tube formation by LEC. Addition of platelets or antibody-mediated crosslinking of podoplanin inhibited LEC migration induced by vascular endothelial growth factors (VEGF-A or VEGF-C), but did not modify basal migration or the response to basic fibroblast growth factor or epidermal growth factor...
July 20, 2017: Platelets
https://www.readbyqxmd.com/read/28727228/actin-gamma-1-a-new-skin-cancer-pathogenic-gene-identified-by-the-biological-feature-based-classification
#6
Xinqian Dong, Yingsheng Han, Zhen Sun, Junlong Xu
Skin cancer is the most common form of cancer that accounting for at least 40% of cancer cases around the world. This study aimed to identify skin cancer-related biological features and predict skin cancer candidate genes by employing machine learning based on biological features of known skin cancer genes. The known skin cancer-related genes were fetched from database and encoded by the enrichment scores of gene ontology and pathways. The optimal features of the skin cancer related genes were selected with a series of feature selection methods, such as mRMR, IFS, and Random Forest algorithm...
July 20, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28726876/bioceramic-nanocomposite-thiol-acrylate-polyhipe-scaffolds-for-enhanced-osteoblastic-cell-culture-in-3d
#7
Aaron Lee, Caitlin R Langford, Luis M Rodriguez-Lorenzo, Helmut Thissen, Neil R Cameron
Emulsion-templated (polyHIPE) scaffolds for bone tissue engineering were produced by photopolymerisation of a mixture of trimethylolpropane tris(3-mercaptopropionate) and dipentaerythritol penta-/hexa-acrylate in the presence of hydroxyapatite (HA) or strontium-modified hydroxyapatite (SrHA) nanoparticles. Porous and permeable polyHIPE materials were produced regardless of the type or incorporation level of the bioceramic, although higher loadings resulted in a larger average pore diameter. Inclusion of HA and SrHA into the scaffolds was confirmed by EDX-SEM, FTIR and XPS and quantified by thermogravimetry...
July 20, 2017: Biomaterials Science
https://www.readbyqxmd.com/read/28726786/calcium-binding-protein-s100a14-induces-differentiation-and-suppresses-metastasis-in-gastric-cancer
#8
Min Zhu, Hongyi Wang, Jiantao Cui, Wenmei Li, Guo An, Yuanming Pan, Qingying Zhang, Rui Xing, Youyong Lu
S100A14 is a calcium-binding protein involved in cell proliferation and differentiation as well as the metastasis of human tumors. In this study, we characterized the regulation of S100A14 expression between biological signatures and clinical pathological features in gastric cancer (GC). Our data demonstrated that S100A14 induced the differentiation of GC by upregulating the expression of E-cadherin and PGII. Moreover, S100A14 expression negatively correlated with cell migration and invasion in in vitro and in vivo experimental models...
July 20, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28726783/kmt2a-promotes-melanoma-cell-growth-by-targeting-htert-signaling-pathway
#9
Changlin Zhang, Chen Song, Tianze Liu, Ranran Tang, Miao Chen, Fan Gao, Binyi Xiao, Ge Qin, Fen Shi, Wenbin Li, Yixin Li, Xiaoyan Fu, Dingbo Shi, Xiangsheng Xiao, Lan Kang, Wenlin Huang, Xiaojun Wu, Bing Tang, Wuguo Deng
Melanoma is an aggressive cutaneous malignancy, illuminating the exact mechanisms and finding novel therapeutic targets are urgently needed. In this study, we identified KMT2A as a potential target, which promoted the growth of human melanoma cells. KMT2A knockdown significantly inhibited cell viability and cell migration and induced apoptosis, whereas KMT2A overexpression effectively promoted cell proliferation in various melanoma cell lines. Further study showed that KMT2A regulated melanoma cell growth by targeting the hTERT-dependent signal pathway...
July 20, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28726720/tgf-%C3%AE-dependent-growth-arrest-and-cell-migration-in-benign-and-malignant-breast-epithelial-cells-are-antagonistically-controlled-by-rac1-and-rac1b
#10
Catharina Melzer, Juliane von der Ohe, Ralf Hass, Hendrik Ungefroren
Despite improvements in diagnosis and treatment, breast cancer is still the most common cancer type among non-smoking females. TGF-β can inhibit breast cancer development by inducing cell cycle arrest in both, cancer cells and, as part of a senescence program in normal human mammary epithelial cells (HMEC). Moreover, TGF-β also drives cell migration and invasion, in part through the small GTPases Rac1 and Rac1b. Depletion of Rac1b or Rac1 and Rac1b in MDA-MB-231 or MDA-MB-435s breast cancer cells by RNA interference enhanced or suppressed, respectively, TGF-β1-induced migration/invasion...
July 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28726396/the-potent-anti-inflammatory-and-anti-adipogenic-properties-of-bamboo-sasa-coreana-nakai-leaves-extract-and-its-major-constituent-flavonoids
#11
Ji Hye Yang, Moon-Hee Choi, Seung Hwa Yang, Sam Seok Cho, Su Jung Park, Hyun-Jae Shin, Sung Hwan Ki
The pro-inflammatory response and recruitment of macrophages into adipose tissue contribute to metabolic dysfunction. Here, we reported the anti-inflammatory and anti-adipogenic effects of the methanol (MeOH) extract and ethylacetate (EtOAc) fraction of bamboo leaf and its molecular mechanism in RAW264.7 cells and 3T3-L1 adipocytes, respectively. Functional macrophage migration assays also were performed. Surprisingly, EtOAc fraction of MeOH extracts from native Korean plant species Sasa coreana Nakai (SCN) has shown potent anti-inflammatory properties; SCN pretreatment inhibited nitric oxide (NO) production (p<0...
July 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28726057/macrophage-migration-inhibitory-factor-mif-modulates-trophic-signaling-through-interaction-with-serine-protease-htra1
#12
Åsa Fex Svenningsen, Svenja Löring, Anna Lahn Sørensen, Ha Uyen Buu Huynh, Simone Hjæresen, Nellie Martin, Jesper Bonnet Moeller, Maria Louise Elkjær, Uffe Holmskov, Zsolt Illes, Malin Andersson, Solveig Beck Nielsen, Eirikur Benedikz
Macrophage migration inhibitory factor (MIF), a small conserved protein, is abundant in the immune- and central nervous system (CNS). MIF has several receptors and binding partners that can modulate its action on a cellular level. It is upregulated in neurodegenerative diseases and cancer although its function is far from clear. Here, we report the finding of a new binding partner to MIF, the serine protease HTRA1. This enzyme cleaves several growth factors, extracellular matrix molecules and is implicated in some of the same diseases as MIF...
July 19, 2017: Cellular and Molecular Life Sciences: CMLS
https://www.readbyqxmd.com/read/28725659/explant-methodology-for-analyzing-neuroblast-migration
#13
Kirsty J Dixon, Alisa Turbic, Ann M Turnley, Daniel J Liebl
The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we describe the isolation of SVZ-derived RMS explants from the neonatal mouse brain, and the conditions required to culture and evaluate their migration.
May 5, 2017: Bio-protocol
https://www.readbyqxmd.com/read/28725484/logarithmic-sensing-in-bacillus-subtilis-aerotaxis
#14
Filippo Menolascina, Roberto Rusconi, Vicente I Fernandez, Steven Smriga, Zahra Aminzare, Eduardo D Sontag, Roman Stocker
Aerotaxis, the directed migration along oxygen gradients, allows many microorganisms to locate favorable oxygen concentrations. Despite oxygen's fundamental role for life, even key aspects of aerotaxis remain poorly understood. In Bacillus subtilis, for example, there is conflicting evidence of whether migration occurs to the maximal oxygen concentration available or to an optimal intermediate one, and how aerotaxis can be maintained over a broad range of conditions. Using precisely controlled oxygen gradients in a microfluidic device, spanning the full spectrum of conditions from quasi-anoxic to oxic (60 n mol/l-1 m mol/l), we resolved B...
2017: NPJ Systems Biology and Applications
https://www.readbyqxmd.com/read/28725241/mir-378-suppresses-the-proliferation-migration-and-invasion-of-colon-cancer-cells-by-inhibiting-sdad1
#15
Mingxi Zeng, Linlin Zhu, Liangping Li, Changming Kang
BACKGROUND: MicroRNAs (miRNAs) play important roles in the growth and metastasis of colon cancer. It is known that one set of miRNAs are dysregulated in colon cancer cells, but the mechanism of their role in cancer development is still largely unknown. Our study focuses on the role of miR-378 in colon cancer cells. METHODS: Human colon cancer tissues and adjacent non-tumor tissues were collected from patients diagnosed in pathological examinations. In addition, human colon cancer cell lines LoVo, CaCo2, SW1116, SW480 and HCT-116, and a normal colonic mucosa cell line NCM460 were included...
2017: Cellular & Molecular Biology Letters
https://www.readbyqxmd.com/read/28725043/mucin-2-silencing-promotes-colon-cancer-metastasis-through-interleukin-6-signaling
#16
Hui-Ping Hsu, Ming-Derg Lai, Jenq-Chang Lee, Meng-Chi Yen, Tzu-Yang Weng, Wei-Ching Chen, Jung-Hua Fang, Yi-Ling Chen
Downregulation of Mucin 2 (MUC2) expression is associated with early carcinogenesis events in colon cancer. MUC2 plays a role in the progression of colon cancer, and reduced MUC2 protein expression correlates with increased interleukin-6 (IL-6) expression. However, the interaction between MUC2 and IL-6 in colorectal cancer metastasis remains unclear. We systematically analyzed MUC2 and IL-6 expression and determined the survival of cancer patients with high or low MUC2 and IL-6 expression using the Oncomine and PrognoScan databases, respectively...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724995/the-expression-of-formyl-peptide-receptor-1-is-correlated-with-tumor-invasion-of-human-colorectal-cancer
#17
Shu-Qin Li, Ning Su, Ping Gong, Hai-Bo Zhang, Jin Liu, Ding Wang, Yan-Ping Sun, Yan Zhang, Feng Qian, Bo Zhao, Yang Yu, Richard D Ye
Formyl peptide receptors (FPRs) are G protein-coupled chemoattractant receptors expressed mainly in phagocytic leukocytes. High expression of FPRs has also been detected in several cancers but the functions of FPR1 in tumor invasion and metastasis is poorly understood. In this study, we investigated the expression of FPRs in primary human colorectal cancer (CRC) and analyzed the association of FPRs expression with clinicopathological parameters. The levels of FPRs mRNA, especially those of FPR1, were significantly higher in colorectal tumors than in distant normal tissues and adjacent non-tumor tissues...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724890/centrosomal-protein-55-activates-nf-%C3%AE%C2%BAb-signalling-and-promotes-pancreatic-cancer-cells-aggressiveness
#18
Tao Peng, Wei Zhou, Feng Guo, He-Shui Wu, Chun-You Wang, Li Wang, Zhi-Yong Yang
Centrosomal protein 55 (CEP55) is a microtubule-bundling protein that participants in cell mitosis. It is overexpressed in several solid tumours and promotes the growth and invasion of cancer cells. However, the role of CEP55 in pancreatic cancer (PANC) remains unclear. Herein, upregulated expression of CEP55 (associated with poor prognosis) was detected in PANC using quantitative real-time reverse transcription PCR, western blotting, and immunohistochemistry. Cell migration, colony formation, wound-healing, and Transwell matrix penetration assays, revealed that upregulation of CEP55 promoted PANC cells proliferation, migration, and invasion in vitro, whereas knockdown of CEP55 attenuated it...
July 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28724635/heterodimers-of-the-transcriptional-factors-nfatc3-and-fosb-mediate-tissue-factor-expression-for-15-s-hydroxyeicosatetraenoic-acid-induced-monocyte-trafficking
#19
Sivareddy Kotla, Nikhlesh K Singh, Daniel Kirchhofer, Gadiparthi N Rao
Tissue factor (TF) is expressed in vascular and nonvascular tissues and functions in several pathways, including embryonic development, inflammation, and cell migration. Many risk factors for atherosclerosis, including hypertension, diabetes, obesity, and smoking, increase TF expression. To better understand the TF-related mechanisms in atherosclerosis, here we investigated the role of 12/15-lipoxygenase (12/15-LOX) in TF expression. 15(S)-hydroxyeicosatetraenoic acid (15(S)-HETE), the major product of human 15-LOXs 1 and 2, induced TF expression and activity in a time-dependent manner in the human monocytic cell line THP1...
July 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28724603/effects-of-microrna-136-on-melanoma-cell-proliferation-apoptosis-and-epithelial-mesenchymal-transition-by-targeting-pmel-through-the-wnt-signaling-pathway
#20
Jiu-Jiang Wang, Zhi-Feng Li, Xiao-Jing Li, Zhao Han, Ling Zhang, Zhi-Jun Liu
The study aims to evaluate the effects of miR-136 on the proliferation, apoptosis and epithelial mesenchymal transition (EMT) of melanoma cells by targeting PMEL through the Wnt signaling pathway. After establishment of melanoma mouse models, melanoma (model group) and normal tissues (normal group) were collected. Immunohistochemistry was performed to determine PMEL protein concentration. Mouse melanoma cells were assigned into control, blank, negative control (NC), miR-136 mimics, miR-136 inhibitors, siRNA-PMEL, and miR-136 inhibitors + siRNA-PMEL, LiC1 (Wnt signaling pathway activator) and siRNA-PMEL+ LiCl groups...
July 19, 2017: Bioscience Reports
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