keyword
MENU ▼
Read by QxMD icon Read
search

Cdc42

keyword
https://www.readbyqxmd.com/read/28811522/the-lichen-secondary-metabolite-atranorin-suppresses-lung-cancer-cell-motility-and-tumorigenesis
#1
Rui Zhou, Yi Yang, So-Yeon Park, Thanh Thi Nguyen, Young-Woo Seo, Kyung Hwa Lee, Jae Hyuk Lee, Kyung Keun Kim, Jae-Seoun Hur, Hangun Kim
Lichens are symbiotic organisms that produce various secondary metabolites. Here, different lichen extracts were examined to identify secondary metabolites with anti-migratory activity against human lung cancer cells. Everniastrum vexans had the most potent inhibitory activity, and atranorin was identified as an active subcomponent of this extract. Atranorin suppressed β-catenin-mediated TOPFLASH activity by inhibiting the nuclear import of β-catenin and downregulating β-catenin/LEF and c-jun/AP-1 downstream target genes such as CD44, cyclin-D1 and c-myc...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805312/the-dock-and-coalesce-mechanism-for-the-association-of-a-wasp-disordered-region-with-the-cdc42-gtpase
#2
Li Ou, Megan Matthews, Xiaodong Pang, Huan-Xiang Zhou
Intrinsically disordered proteins (IDPs) play key roles in signaling and regulation. Many IDPs undergo folding upon binding to their targets. We have proposed that coupled folding and binding of IDPs generally follow a dock-and-coalesce mechanism, whereby a segment of the IDP, through diffusion, docks to its cognate subsite and, subsequently, the remaining segments coalesce around their subsites. Here, by a combination of experiment and computation, we determined the precise form of dock-and-coalesce operating in the association between the intrinsically disordered GTPase binding domain (GBD) of the Wiskott-Aldrich Syndrome protein (WASP) and the Cdc42 GTPase...
August 14, 2017: FEBS Journal
https://www.readbyqxmd.com/read/28800782/effects-of-chinese-medicinal-herbs-on-expression-of-brain-derived-neurotrophic-factor-bdnf-and-its-interaction-with-human-breast-cancer-mda-mb-231-cells-and-endothelial-huvecs
#3
Jen-Hwey Chiu, Fang-Pey Chen, Yi-Fang Tsai, Man-Ting Lin, Ling-Ming Tseng, Yi-Ming Shyr
BACKGROUND: Our previous study demonstrated that an up-regulation of the Brain-Derived Neurotrophic Factor (BDNF) signaling pathway is involved the mechanism causing the recurrence of triple negative breast cancer. The aim of this study is to investigate the effects of commonly used Chinese medicinal herbs on MDA-MB-231 and HUVEC cells and how they interact with BDNF. METHODS: Human TNBC MDA-MB-231 cells and human endothelial HUVEC cells were used to explore the effect of commonly used Chinese herbal medicines on cancer cells alone, on endothelial cells alone and on cancer cell/endothelial cell interactions; this was done via functional studies, including migration and invasion assays...
August 12, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28776765/s-cerevisiae-gle2-rae1-is-involved-in-septin-organization-essential-for-cell-cycle-progression
#4
Gesa Zander, Wilfried Kramer, Anika Seel, Heike Krebber
Gle2/Rae1 is highly conserved from yeast to humans and described as an mRNA export factor. Additionally, it is implicated in the anaphase-promoting complex (APC)-mediated cell cycle regulation in higher eukaryotes. Here we identified an involvement for S. cerevisiae Gle2 in septin organization, which is crucial for cell cycle progression and cell division. Gle2 genetically and physically interacts with components of the septin-ring. Importantly, deletion of GLE2 leads to elongated buds, severe defects in septin-assembly and their cellular mislocalization...
August 4, 2017: Yeast
https://www.readbyqxmd.com/read/28765773/astaxanthin-induces-migration-in-human-skin-keratinocytes-via-rac1-activation-and-rhoa-inhibition
#5
Dakanda Ritto, Supita Tanasawet, Sawana Singkhorn, Wanwimol Klaypradit, Pilaiwanwadee Hutamekalin, Varomyalin Tipmanee, Wanida Sukketsiri
BACKGROUND/OBJECTIVES: Re-epithelialization has an important role in skin wound healing. Astaxanthin (ASX), a carotenoid found in crustaceans including shrimp, crab, and salmon, has been widely used for skin protection. Therefore, we investigated the effects of ASX on proliferation and migration of human skin keratinocyte cells and explored the mechanism associated with that migration. MATERIAL/METHOD: HaCaT keratinocyte cells were exposed to 0.25-1 µg/mL of ASX...
August 2017: Nutrition Research and Practice
https://www.readbyqxmd.com/read/28759015/peptidomimetic-inhibitors-of-apc-asef-interaction-block-colorectal-cancer-migration
#6
Haiming Jiang, Rong Deng, Xiuyan Yang, Jialin Shang, Shaoyong Lu, Yanlong Zhao, Kun Song, Xinyi Liu, Qiufen Zhang, Yu Chen, Y Eugene Chinn, Geng Wu, Jian Li, Guoqiang Chen, Jianxiu Yu, Jian Zhang
The binding of adenomatous polyposis coli (APC) to its receptor Asef relieves the negative intramolecular regulation of Asef and leads to aberrant cell migration in human colorectal cancer. Because of its crucial role in metastatic dissemination, the interaction between APC and Asef is an attractive target for anti-colorectal-cancer therapy. We rationally designed a series of peptidomimetics that act as potent inhibitors of the APC interface. Crystal structures and biochemical and cellular assays showed that the peptidomimetics in the APC pocket inhibited the migration of colorectal cells by disrupting APC-Asef interaction...
July 24, 2017: Nature Chemical Biology
https://www.readbyqxmd.com/read/28754687/cells-lay-their-own-tracks-optogenetic-cdc42-activation-stimulates-fibronectin-deposition-supporting-directed-migration
#7
Seth P Zimmerman, Sreeja B Asokan, Brian Kuhlman, James E Bear
Rho GTPase family members are known regulators of directed migration and therefore play key roles in processes including development, immune response and cancer metastasis. However, their individual contributions to these processes are complex. Here, we regulate the activity of two family members, Rac and Cdc42, by optogenetically recruiting specific GEF DH/PH domains to defined regions on the cell membrane. We find that the localized activation of both GTPases produce lamellipodia in cells plated on a fibronectin substrate...
July 28, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28753202/targeting-the-cxcr4-cxcl12-axis-in-treating-epithelial-ovarian-cancer
#8
T L Mao, K F Fan, C L Liu
Ovarian carcinoma is the most crucial and difficult target for available therapeutic treatments among gynecological malignancies, and great efforts are required to find an effective solution. Molecular studies showed that the chemokine stromal cell-derived factor (SDF)-1 (also known as CXCL12) and its receptor, CXCR4, are key determinants of tumor initiation, progression, and metastasis in ovarian carcinomas. Hence, it is generally believed that blocking the CXCR4/CXCL12 pathway could serve as a potential therapy for patients with ovarian cancer...
July 28, 2017: Gene Therapy
https://www.readbyqxmd.com/read/28751779/mir-199a-inhibits-secondary-envelopment-of-herpes-simplex-virus-1-through-the-downregulation-of-cdc42-specific-gtpase-activating-protein-localized-in-golgi-apparatus
#9
Kyousuke Kobayashi, Fumiko Suemasa, Hiroshi Sagara, Shinya Nakamura, Yasushi Ino, Kazuyoshi Kobayashi, Hiroaki Hiramatsu, Takeshi Haraguchi, Kazuo Kurokawa, Tomoki Todo, Akihiko Nakano, Hideo Iba
Because several studies have shown that exogenous miR-199a has antiviral effects against various viruses, including herpesviruses, we examined how miR-199a exerts its antiviral effects using epithelial tumour cell lines infected with herpes simplex virus-1 (HSV-1). We found that both miR-199a-5p and -3p impair the secondary envelopment of HSV-1 by suppressing their common target, ARHGAP21, a Golgi-localized GTPase-activating protein for Cdc42. We further found that the trans-cisternae of the Golgi apparatus are a potential membrane compartment for secondary envelopment...
July 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28749339/pten-controls-glandular-morphogenesis-through-a-juxtamembrane-%C3%AE-arrestin1-arhgap21-scaffolding-complex
#10
Arman Javadi, Ravi K Deevi, Emma Evergren, Elodie Blondel-Tepaz, George S Baillie, Mark Gh Scott, Frederick Charles Campbell
PTEN controls three-dimensional (3D) glandular morphogenesis by coupling juxtamembrane signalling to mitotic spindle machinery. While molecular mechanisms remain unclear, PTEN interacts through its C2 membrane-binding domain with the scaffold protein β-Arrestin1. Because β-Arrestin1 binds and suppresses the Cdc42 GTPase-activating protein ARHGAP21, we hypothesize that PTEN controls Cdc42-dependent morphogenic processes through a β-Arrestin1-ARHGAP21 complex. Here we show that PTEN knockdown (KD) impairs β-Arrestin1 membrane localization, β-Arrestin1-ARHGAP21 interactions, Cdc42 activation, mitotic spindle orientation and 3D glandular morphogenesis...
July 27, 2017: ELife
https://www.readbyqxmd.com/read/28748534/mir-130b-directly-targets-arhgap1-to-drive-activation-of-a-metastatic-cdc42-pak1-ap1-positive-feedback-loop-in-ewing-sarcoma
#11
Laura Satterfield, Ryan Shuck, Lyazat Kurenbekova, Wendy Allen-Rhoades, Dean Edwards, Shixia Huang, Kimal Rajapakshe, Cristian Coarfa, Lawrence A Donehower, Jason T Yustein
Ewing Sarcoma (ES) is a highly aggressive bone tumor with peak incidence in the adolescent population. It has a high propensity to metastasize, which is associated with dismal survival rates of approximately 25%. To further understand mechanisms of metastasis we investigated microRNA regulatory networks in ES. Our studies focused on miR-130b due to our analysis that enhanced expression of this microRNA has clinical relevance in multiple sarcomas, including ES. Our studies provide insights into a novel positive feedback network involving the direct regulation of miR-130b and activation of downstream signaling events contributing towards sarcoma metastasis...
July 27, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28747344/the-small-rho-gtpase-tc10-modulates-b-cell-immune-responses
#12
Marianne Burbage, Selina J Keppler, Beatriz Montaner, Pieta K Mattila, Facundo D Batista
Rho family GTPases regulate diverse cellular events, such as cell motility, polarity, and vesicle traffic. Although a wealth of data exists on the canonical Rho GTPases RhoA, Rac1, and Cdc42, several other family members remain poorly studied. In B cells, we recently demonstrated a critical role for Cdc42 in plasma cell differentiation. In this study, we focus on a close homolog of Cdc42, TC10 (also known as RhoQ), and investigate its physiological role in B cells. By generating a TC10-deficient mouse model, we show that despite reduced total B cell numbers, B cell development in these mice occurs normally through distinct developmental stages...
July 26, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28742002/cdc42-activation-state-affects-its-localization-and-protein-levels-in-fission-yeast
#13
Miguel Estravís, Sergio Antonio Rincón, Elvira Portales, Pilar Pérez, Beatriz Santos
Rho GTPases control polarized cell growth and are well-known regulators of exocytic and endocytic processes. Cdc42 is an essential GTPase, conserved from yeast to humans, that is critical for cell polarization. Cdc42 is negatively regulated by the GTPase-activating proteins (GAPs) and the GDP dissociation inhibitors (GDIs), and positively regulated by guanine nucleotide exchange factors (GEFs). Cdc42 GTPase can be found in a GTP- or GDP-bound state, which determines the ability to bind downstream effector proteins and activate signalling pathways...
July 26, 2017: Microbiology
https://www.readbyqxmd.com/read/28739799/lgr5-receptor-promotes-cell-cell-adhesion-in-stem-cells-and-colon-cancer-cells-via-the-iqgap1-rac1-pathway
#14
Kendra S Carmon, Xing Gong, Jing Yi, Ling Wu, Anthony Thomas, Catherine M Moore, Ikuo Masuho, David J Timson, Kirill A Martemyanov, Qingyun J Liu
Leucine rich repeat containing G protein coupled receptor 5 (LGR5) is a bona fide marker of adult stem cells in several epithelial tissues, most notably in the intestinal crypts and is highly upregulated in many colorectal, hepatocellular, and ovarian cancers. LGR5 activation by R-spondin (RSPO) ligands potentiates Wnt/β-catenin signaling in vitro, yet deletion of LGR5 in stem cells has little or no effect on Wnt/β-catenin signaling or cell proliferation in vivo. Remarkably, modulation of LGR5 expression has a major impact on the actin cytoskeletal structure and cell adhesion in the absence of RSPO stimulation, but the molecular mechanism is unclear...
July 24, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28739485/hsp90-is-necessary-for-the-ack1-dependent-phosphorylation-of-stat1-and-stat3
#15
Nisintha Mahendrarajah, Marina E Borisova, Sigrid Reichardt, Maren Godmann, Andreas Sellmer, Siavosh Mahboobi, Andrea Haitel, Katharina Schmid, Lukas Kenner, Thorsten Heinzel, Petra Beli, Oliver H Krämer
Signal transducers and activators of transcription (STATs) are latent, cytoplasmic transcription factors. Janus kinases (JAKs) and activated CDC42-associated kinase-1 (ACK1/TNK2) catalyse the phosphorylation of STAT1 and the expression of its target genes. Here we demonstrate that catalytically active ACK1 promotes the phosphorylation and nuclear accumulation of STAT1 in transformed kidney cells. These processes are associated with STAT1-dependent gene expression and an interaction between endogenous STAT1 and ACK1...
July 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28739484/protein-kinase-c%C3%AE-%C3%AE-is-dispensable-for-platelet-function-in-thrombosis-and-hemostasis-in-mice
#16
Sarah Beck, Michael Leitges, David Stegner
Platelet activation at sites of vascular injury is crucial for hemostasis, but it may also cause myocardial infarction or ischemic stroke. Upon platelet activation, cytoskeletal reorganization is essential for platelet secretion and thrombus formation. Members of the protein kinase C family, which includes 12 isoforms, are involved in most platelet responses required for thrombus formation. The atypical protein kinase Cι/λ (PKCι/λ) has been implicated as an important mediator of cell polarity, carcinogenesis and immune cell responses...
July 21, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28720386/cdc42-interacting-protein-4-silencing-relieves-pulmonary-fibrosis-in-stz-induced-diabetic-mice-via-the-wnt-gsk-3%C3%AE-%C3%AE-catenin-pathway
#17
Xiaoping Zhang, Ying Liu, Runxia Shao, Wei Li
Cdc42-interacting protein-4 (CIP4) has been reported to be closely associated with diabetic nephropathy in rat. However, little is known about the correlation between CIP4 and diabetic pulmonary fibrosis (PF) in mice. Here, diabetes was induced by streptozotocin (STZ), and later lung tissue was collected and subjected to hematoxylin and eosin (H & E) staining for morphological examination. The distinct up-regulation of CIP4 was observed in diabetic PF mice. CIP4 silencing increased overall weight and decreased lung weight...
July 15, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28707808/cytotoxic-necrotizing-factor-1-promotes-prostate-cancer-progression-through-activating-the-cdc42-pak1-axis
#18
Yaxiu Guo, Zhisong Zhang, Huiting Wei, Jingyu Wang, Junqiang Lv, Kai Zhang, Evan T Keller, Zhi Yao, Quan Wang
Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections and plays a role in prostatic carcinogenesis and prostate cancer (PCa) progression. However, the mechanisms through which UPEC promotes PCa development and progression are unclear. Cytotoxic necrotizing factor 1 (CNF1) is one of the most important UPEC toxins and its role in PCa progression has never been studied. We found that UPEC-secreted CNF1 promoted the migration and invasion of PCa cells and PCa metastasis. In vitro studies showed that CNF1 promotes pro-migratory and pro-invasive activity through entering PCa cells and activating Cdc42, which subsequently induced PAK1 phosphorylation and upregulation of MMP-9 expression...
July 14, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/28701658/drosophila-dock-family-protein-zizimin-involves-in-pigment-cell-differentiation-in-pupal-retinae
#19
Fumito Ozasa, Kazushige Morishita, Ngoc Anh Suong Dang, Seiji Miyata, Hideki Yoshida, Masamitsu Yamaguchi
The dedicator of cytokinesis (DOCK) family proteins are known as one of guanine nucleotide exchange factors (GEFs), that contribute to cellular signaling processes by activating small G proteins. Although mammalian Zizimin is known to be a GEF for Cdc42 of Rho family small GTPase, its role in vivo is not well understood. Here we studied in vivo function of Drosophila Zizimin (Ziz). Knockdown of Ziz in eye imaginal discs induced the rough eye phenotype accompanied with fusion of ommatidia, loss of bristles and loss of pigments...
July 13, 2017: Cell Structure and Function
https://www.readbyqxmd.com/read/28697804/ang-ii-at2r-increases-mesenchymal-stem-cell-migration-by-signaling-through-the-fak-and-rhoa-cdc42-pathways-in-vitro
#20
Xiu-Ping Xu, Hong-Li He, Shu-Ling Hu, Ji-Bin Han, Li-Li Huang, Jing-Yuan Xu, Jian-Feng Xie, Ai-Ran Liu, Yi Yang, Hai-Bo Qiu
BACKGROUND: Mesenchymal stem cells (MSCs) migrate via the bloodstream to sites of injury and are possibly attracted by inflammatory factors. As a proinflammatory mediator, angiotensin II (Ang II) reportedly enhances the migration of various cell types by signaling via the Ang II receptor in vitro. However, few studies have focused on the effects of Ang II on MSC migration and the underlying mechanisms. METHODS: Human bone marrow MSCs migration was measured using wound healing and Boyden chamber migration assays after treatments with different concentrations of Ang II, an AT1R antagonist (Losartan), and/or an AT2R antagonist (PD-123319)...
July 12, 2017: Stem Cell Research & Therapy
keyword
keyword
14398
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"