keyword
MENU ▼
Read by QxMD icon Read
search

phosphatidyl serine

keyword
https://www.readbyqxmd.com/read/28542284/haemocytes-collected-from-experimentally-infected-pacific-oysters-crassostrea-gigas-detection-of-ostreid-herpesvirus-1-dna-rna-and-proteins-in-relation-with-inhibition-of-apoptosis
#1
Claire Martenot, Ophélie Gervais, Bruno Chollet, Maryline Houssin, Tristan Renault
Recent transcriptomic approaches focused on anti-viral immunity in molluscs lead to the assumption that the innate immune system, such as apoptosis, plays a crucial role against ostreid herpesvirus type 1 (OsHV-1), infecting Pacific cupped oyster, Crassostrea gigas. Apoptosis constitutes a major mechanism of anti-viral response by limiting viral spread and eliminating infected cells. In this way, an OsHV-1 challenge was performed and oysters were monitored at three times post injection to investigate viral infection and host response: 2h (early after viral injection in the adductor muscle), 24h (intermediate time), and 48h (just before first oyster mortality record)...
2017: PloS One
https://www.readbyqxmd.com/read/28490634/inflammatory-cytokines-down-regulate-the-barrier-protective-prostasin-matriptase-proteolytic-cascade-early-in-experimental-colitis
#2
Marguerite S Buzza, Tierra A Johnson, Gregory D Conway, Erik W Martin, Subhradip Mukhopadhyay, Terez Shea-Donohue, Toni M Antalis
Compromised gastrointestinal barrier function is strongly associated with the progressive and destructive pathologies of the two main forms of IBD, Ulcerative Colitis (UC) and Crohns disease (CD). Matriptase is a membrane-anchored serine protease encoded by Suppression of Tumorigenicity-14 (ST14) gene, which is critical for epithelial barrier development and homeostasis. Matriptase barrier protective activity is linked with the glycosyl-phosphatidyl-inositol (GPI)-anchored serine protease prostasin, which is a co-factor for matriptase zymogen activation...
May 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28472616/stereochemistry-and-concentration-dependent-effects-of-phosphatidylserine-enrichment-on-platelet-function
#3
Audrey F Meyer, Sarah M Gruba, Donghyuk Kim, Ben M Meyer, Secil Koseoglu, Joseph J Dalluge, Christy L Haynes
Platelets are small (1-2μm in diameter), circulating anuclear cell fragments with important roles in hemostasis and thrombosis that provide an excellent platform for studying the role of membrane components in cellular communication. Platelets use several forms of communication including exocytosis of three distinct granule populations, formation of bioactive lipid mediators, and shape change (allowing for adhesion). This work explores the role of stereochemistry and concentration of exogenous phosphatidylserine (PS) on platelet exocytosis and adhesion...
May 1, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28448535/alpha-1-antitrypsin-supplementation-improves-alveolar-macrophages-efferocytosis-and-phagocytosis-following-cigarette-smoke-exposure
#4
Karina A Serban, Daniela N Petrusca, Andrew Mikosz, Christophe Poirier, Angelia D Lockett, Lauren Saint, Matthew J Justice, Homer L Twigg, Michael A Campos, Irina Petrache
Cigarette smoking (CS), the main risk factor for COPD (chronic obstructive pulmonary disease) in developed countries, decreases alveolar macrophages (AM) clearance of both apoptotic cells and bacterial pathogens. This global deficit of AM engulfment may explain why active smokers have worse outcomes of COPD exacerbations, episodes characterized by airway infection and inflammation that carry high morbidity and healthcare cost. When administered as intravenous supplementation, the acute phase-reactant alpha-1 antitrypsin (A1AT) reduces the severity of COPD exacerbations in A1AT deficient (AATD) individuals and of bacterial pneumonia in murine models, but the effect of A1AT on AM scavenging functions has not been reported...
2017: PloS One
https://www.readbyqxmd.com/read/28322991/thyroid-hormone-induce-a-p53-dependent-dna-damage-through-pi3k-akt-activation-in-sperm
#5
Hong Xian, Fang Wang, Wending Teng, Dong Yang, Ming Zhang
Accumulating evidences showed that thyroid hormone was participated in the functioning of the reproductive system, and an elevated level of thyroid hormones had a negative impact on reproductive system. However, the molecular basis for this observation still remains to be fully understood. Here, we show that l-Thyroxine significantly impaired human sperm motility. The molecular basis showed that thyroxine receptor stimulation triggers Phosphatidyl Inositol 3-kinase (PI3K)/Akt signaling activation leading to the E3 ligase MDM2 phosphorylation at serine 166, which directly interacted with p53 for degradation...
June 5, 2017: Gene
https://www.readbyqxmd.com/read/28306533/ros-mediated-induction-of-apoptosis-by-benzoquinone-embelin-in-human-colon-adenocarcinoma-cells-ht-29
#6
Kodandaram Sumalatha, Mohan Gowda, Sreepriya Meenakshisundaram
Background Embelin is a benzoquinone reported to possess anticancer activity in several in vivo and in vitro models of carcinogenesis, especially hematopoietic and prostate malignancy. A detailed investigation on the influence of embelin on epithelial malignancy model system, especially colon adenocarcinoma, is lacking. The objective of the current study is to investigate the antiproliferative, antiinvasive and proapoptotic potential of embelin on colon adenocarcinoma cell line HT-29. Methods The effect of embelin (35 µg/mL for 24 h) on cell proliferation was assessed by Sulforhodamine B assay and bromodeoxyuridine incorporation test, antiinvasive effect by Boyden chamber assay and scratch assay...
March 17, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28286882/a-study-to-investigate-the-role-of-gulp-ced-6-gene-in-eat-me-signaling-in-cellular-efferocytosis-and-immunosurveillance
#7
H Banerjee, B Bazemore, A Barfield, D Crummity, C Krauss, G Payne, J Rousch, V Manglik
In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various "eat me" signals on these cells including Phosphatidyl serine (PtdSer) expression, nitric oxide (NO) signaling and Leukotrine B4 (LTB4) expression and Caspase 3 activation. Investigations were done by fluorescence microscopy techniques, ELISA assay and colorimetric assays using a standard microplate reader and spectrophotometer...
December 2016: Immunological Disorders & Immunotherapy
https://www.readbyqxmd.com/read/28286004/computational-modeling-reveals-that-signaling-lipids-modulate-the-orientation-of-k-ras4a-at-the-membrane-reflecting-protein-topology
#8
Zhen-Lu Li, Matthias Buck
The structural, dynamical, and functional characterization of the small GTPase K-Ras has become a research area of intense focus due to its high occurrence in human cancers. Ras proteins are only fully functional when they interact with the plasma membrane. Here we present all-atom molecular dynamics simulations (totaling 5.8 μs) to investigate the K-Ras4A protein at membranes that contain anionic lipids (phosphatidyl serine or phosphatidylinositol bisphosphate). We find that similarly to the homologous and highly studied K-Ras4B, K-Ras4A prefers a few distinct orientations at the membrane...
April 4, 2017: Structure
https://www.readbyqxmd.com/read/28273904/methionine-aminopeptidase-2-is-a-key-regulator-of-apoptotic-like-cell-death-in-leishmania-donovani
#9
Ritesh Kumar, Kartikeya Tiwari, Vikash Kumar Dubey
We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28123531/goniothalamin-induces-mitochondria-mediated-apoptosis-associated-with-endoplasmic-reticulum-stress-induced-activation-of-jnk-in-hela-cells
#10
Thanet Sophonnithiprasert, Wilawan Mahabusarakam, Yukio Nakamura, Ramida Watanapokasin
Goniothalamin, a natural occurring styryl-lactone isolated from Goniothalamus macrophyllus (Blume) Hook. f. & Thomson var. macrophyllus, can trigger cancer cell death in various types of cancer cell. The present study focused on elucidation of the mitochondria-mediated apoptosis associated with endoplasmic reticulum (ER) stress-induced activation of c-Jun NH2-terminal kinase (JNK) by goniothalamin in HeLa cervical cancer cells. Cell viability was determined using an MTT assay, and DNA condensation and loss of mitochondrial membrane potential were determined using Hoechst 33342 and JC-1 staining, respectively...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/27896225/the-urokinase-urokinase-receptor-system-in-mast-cells-effects-of-its-functional-interaction-with-fmlf-receptors
#11
Francesca Wanda Rossi, Nella Prevete, Felice Rivellese, Filomena Napolitano, Nunzia Montuori, Loredana Postiglione, Carmine Selleri, Amato de Paulis
Mast cell and basophils express the high affinity receptor for IgE (FcɛRI) and are primary effector cells of allergic disorders. The urokinase (uPA)-mediated plasminogen activation system is involved in physiological and pathological events based on cell migration and tissue remodelling, such as inflammation, wound healing, angiogenesis and metastasis. uPA is a serine protease that binds uPAR, a high affinity glycosyl-phosphatidyl-inositol (GPI)-anchored receptor. uPAR focuses uPA activity at the cell surface and activates intracellular signaling through lateral interactions with integrins, receptor tyrosine kinases and the G-protein-coupled family of fMLF chemotaxis receptors (FPRs)...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27869259/the-effect-of-top-layer-chemistry-on-the-formation-of-supported-lipid-bilayers-on-polyelectrolyte-multilayers-primary-versus-quaternary-amines
#12
E Diamanti, P Andreozzi, R Anguiano, L Yate, D Gregurec, N Politakos, R F Ziolo, E Donath, S E Moya
The influence of the surface chemistry of polyelectrolyte multilayers (PEMs) on the formation of lipid bilayers is studied here for PEMs with either polyallylamine hydrochloride (PAH) or polydiallyldimethylammonium chloride (PDADMAC) as a polycation as a top layer, and polystyrene sulfonate (PSS) as a polyanion. Small unilamellar vesicles (SUVs) composed of phosphatidyl choline and phosphatidyl serine at a 50 : 50 molar ratio are deposited on top of the PEM films. The assembly of the SUVs into bilayers is studied via a quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP)...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27864176/insights-into-the-biosynthesis-of-duramycin
#13
Liujie Huo, Ayşe Ökesli, Ming Zhao, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are characterized by the thioether cross-linked bisamino acids lanthionine (Lan) and methyllanthionine (MeLan). Duramycin contains 19 amino acids, including one Lan and two MeLans, an unusual lysinoalanine (Lal) bridge formed from the ε-amino group of lysine 19 and a serine residue at position 6, and an erythro-3-hydroxy-l-aspartic acid at position 15. These modifications are important for the interactions of duramycin with its biological target, phosphatidylethanolamine (PE)...
February 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27836661/phosphorylation-of-poly-rc-binding-protein-1-pcbp1-contributes-to-stabilization-of-mu-opioid-receptor-mor-mrna-via-interaction-with-au-rich-element-rna-binding-protein-1-auf1-and-poly-a-binding-protein-pabp
#14
Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, Horace H Loh
Gene regulation at the post-transcriptional level is frequently based on cis- and trans-acting factors on target mRNAs. We found a C-rich element (CRE) in mu-opioid receptor (MOR) 3'-untranslated region (UTR) to which poly (rC) binding protein 1 (PCBP1) binds, resulting in MOR mRNA stabilization. RNA immunoprecipitation and RNA EMSA revealed the formation of PCBP1-RNA complexes at the element. Knockdown of PCBP1 decreased MOR mRNA half-life and protein expression. Stimulation by forskolin increased cytoplasmic localization of PCBP1 and PCBP1/MOR 3'-UTR interactions via increased serine phosphorylation that was blocked by protein kinase A (PKA) or (phosphatidyl inositol-3) PI3-kinase inhibitors...
January 20, 2017: Gene
https://www.readbyqxmd.com/read/27824861/identification-of-biomarkers-of-impaired-sensory-profiles-among-autistic-patients
#15
Afaf El-Ansary, Wail M Hassan, Hanan Qasem, Undurti N Das
BACKGROUND: Autism is a neurodevelopmental disorder that displays significant heterogeneity. Comparison of subgroups within autism, and analyses of selected biomarkers as measure of the variation of the severity of autistic features such as cognitive dysfunction, social interaction impairment, and sensory abnormalities might help in understanding the pathophysiology of autism. METHODS AND PARTICIPANTS: In this study, two sets of biomarkers were selected. The first included 7, while the second included 6 biomarkers...
2016: PloS One
https://www.readbyqxmd.com/read/27712999/evaluation-of-the-cytotoxic-apoptosis-inducing-activity-and-molecular-docking-of-spiroquinazolinone-benzamide-derivatives-in-mcf-7-breast-cancer-cells
#16
Majid Mahdavi, Malihe Mohseni Lavi, Reza Yekta, Mohammad Amin Moosavi, Mahnaz Nobarani, Saeed Balalaei, Sanam Arami, Mohammad Reza Rashidi
Previous studies have suggested that quinazolinone derivatives are potent apoptosis-inducing agents in various cancer cell lines. In the present study, we have investigated cytotoxic, apoptosis induction, and molecular docking activities of the spiroquinazolinone benzamide derivatives family on MCF-7 human breast cancer cells. The MTT cytotoxicity assays and docking studies showed that 4t-CHQB was the most active compound among the prepared spiroquinazolinone benzamide compounds with IC50 of 50 ± 1.2 μM and was selected for further assessments...
December 25, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27624766/total-%C3%AE-synuclein-levels-in-human-blood-cells-csf-and-saliva-determined-by-a-lipid-elisa
#17
Suaad Abd-Elhadi, Misericordia Basora, Dolores Vilas, Eduardo Tolosa, Ronit Sharon
The validity of α-synuclein (α-Syn) as a biomarker for Parkinson's disease (PD) is still under investigation. Conventional methods for capture and quantitation of α-Syn protein in human samples are primarily based on anti-α-Syn antibodies. Specific and competent antibodies were raised against α-Syn. However, capture by anti-α-Syn antibodies may be limited to specific epitope recognition, attributed to protein structure or post-translational modifications. Hence, antibody-based methods for α-Syn capture raise a concern regarding their efficacy to detect the intracellular, unfolded α-Syn pool...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27502061/detection-of-myocarditis-with-molecular-echo-imaging-another-potential-application-for-the-phosphatidyl-serine-microbubble
#18
EDITORIAL
Thomas R Porter
No abstract text is available yet for this article.
August 2016: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/27473012/identification-of-a-secretory-phospholipase-a2-from-papaver-somniferum-l-that-transforms-membrane-phospholipids
#19
Veronika Jablonická, Johanna Mansfeld, Ingo Heilmann, Marek Obložinský, Mareike Heilmann
The full-length sequence of a new secretory phospholipase A2 was identified in opium poppy seedlings (Papaver somniferum L.). The cDNA of poppy phospholipase A2, denoted as pspla2, encodes a protein of 159 amino acids with a 31 amino acid long signal peptide at the N-terminus. PsPLA2 contains a PLA2 signature domain (PA2c), including the Ca(2+)-binding loop (YGKYCGxxxxGC) and the catalytic site motif (DACCxxHDxC) with the conserved catalytic histidine and the calcium-coordinating aspartate residues. The aspartate of the His/Asp dyad playing an important role in animal sPLA2 catalysis is substituted by a serine residue...
September 2016: Phytochemistry
https://www.readbyqxmd.com/read/27449044/sperm-associated-antigen-9-spag9-promotes-the-survival-and-tumor-growth-of-triple-negative-breast-cancer-cells
#20
Nirmala Jagadish, Namita Gupta, Sumit Agarwal, Deepak Parashar, Aditi Sharma, Rukhsar Fatima, Amos Prashant Topno, Vikash Kumar, Anil Suri
Recently, we demonstrated the association of sperm-associated antigen 9 (SPAG9) expression with breast cancer. Among breast cancer, 15 % of the cancers are diagnosed as triple-negative breast cancers (TNBC) based on hormone receptor status and represent an important clinical challenge because of lack of effective available targeted therapy. Therefore, in the present investigation, plasmid-based small hairpin (small hairpin RNA (shRNA)) approach was used to ablate SPAG9 in aggressive breast cancer cell line model (MDA-MB-231) in order to understand the role of SPAG9 at molecular level in apoptosis, cell cycle, and epithelial-to-mesenchymal transition (EMT) signaling...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
keyword
keyword
14393
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"