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phosphatidyl serine

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https://www.readbyqxmd.com/read/29665555/hesperidin-promotes-programmed-cell-death-by-downregulation-of-nongenomic-estrogen-receptor-signalling-pathway-in-endometrial-cancer-cells
#1
Z B Cincin, B Kiran, Y Baran, B Cakmakoglu
Endometrial carcinoma (EC) is the most common malignant gynecologic tumor in women. EC is thought to be caused by increasing estrogen levels relative to progesterone in the body. Hesperidin (Hsd), a biologically active flavonoid, could be extracted from Citrus species. It has been recently shown that Hsd could exert anticarcinogenic properties in different cancer types. However, the effects of Hsd and its molecular mechanisms on EC remain unclear. In this study, the antiproliferative, apoptotic and genomic effects of Hsd in EC and its underlying mechanisms were identified...
April 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29622550/14-3-3-proteins-in-platelet-biology-and-glycoprotein-ib-ix-signaling
#2
Yunfeng Chen, Zaverio M Ruggeri, Xiaoping Du
Members of the 14-3-3 family of proteins function as adapters/modulators that recognize phosphoserine/phosphothreonine-based binding motifs in many intracellular proteins and play fundamental roles in signal transduction pathways of eukaryotic cells. In platelets, 14-3-3 plays a wide range of regulatory roles in phosphorylation-dependent signaling pathways, including G-protein signaling, cAMP signaling, agonist-induced phosphatidyl-serine (PS) exposure and regulation of mitochondrial function. In particular, 14-3-3 inter-acts with several phosphoserine-dependent binding sites in the major platelet adhesion re-ceptor, the glycoprotein Ib-IX complex (GPIb-IX), regulating its interaction with von Wil-lebrand factor (VWF) and mediating VWF/GPIb-IX-dependent mechano-signal transduc-tion leading to platelet activation...
April 5, 2018: Blood
https://www.readbyqxmd.com/read/29486557/dual-functions-of-lip6-and-its-regulation-of-lipid-metabolism-in-the-oleaginous-fungus-mucor-circinelloides
#3
Xinyi Zan, Xin Tang, Linfang Chu, Yuanda Song
Although the multiple roles of lipases have been reported in yeasts and microalgae, the functions of lipases have not been studied in oleaginous filamentous fungi. Lipase Lip6 has been reported in the oleaginous filamentous fungus Mucor circinelloides with the consensus lipase motif GXSXG and the typical acyltransferase motif of H-(X)4-D. To demonstrate that Lip6 might play dual roles as a lipase and an acyltransferase, we performed site-directed mutagenesis in the lipase motif and the acyltransferase motif of Lip6...
February 28, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29474406/the-role-of-the-folate-pathway-in-pancreatic-cancer-risk
#4
Shirisha Chittiboyina, Zhongxue Chen, E Gabriela Chiorean, Lisa M Kamendulis, Barbara A Hocevar
BACKGROUND: Pancreatic cancer is the third leading cause of cancer related deaths in the United States. Several dietary factors have been identified that modify pancreatic cancer risk, including low folate levels. In addition to nutrition and lifestyle determinants, folate status may be influenced by genetic factors such as single nucleotide polymorphisms (SNPs). In the present study, we investigated the association between folate levels, genetic polymorphisms in genes of the folate pathway, and pancreatic cancer...
2018: PloS One
https://www.readbyqxmd.com/read/29462602/6-shogaol-induces-caspase-independent-paraptosis-in-cancer-cells-via-proteasomal-inhibition
#5
Divya Nedungadi, Anupama Binoy, Nanjan Pandurangan, Sanjay Pal, Bipin G Nair, Nandita Mishra
An α, β-unsaturated carbonyl compound of ginger, 6-Shogaol (6S), induced extensive cytoplasmic vacuolation and cell death in breast cancer cell (MDA-MB-231) and non-small lung cancer (A549) cells. In the presence of autophagic inhibitors the cells continued to exhibit cytoplasmic vacuolation and cell death clearly distinguishing it from the classic autophagic process. 6S induced death did not exhibit the characteristic apoptotic features like caspase cleavage, phosphatidyl serine exposure and DNA fragmentation...
February 17, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29427248/fabrication-of-3-o-sn-phosphatidyl-l-serine-anchored-plga-nanoparticle-bearing-amphotericin-b-for-macrophage-targeting
#6
Pankaj K Singh, Anil K Jaiswal, Vivek K Pawar, Kavit Raval, Animesh Kumar, Himangsu K Bora, Anuradha Dube, Manish K Chourasia
PURPOSE: To fabricate, characterize and evaluate 3-O-sn-Phosphatidyl-L-serine (PhoS) anchored PLGA nanoparticles for macrophage targeted therapeutic intervention of VL. MATERIALS AND METHODS: PLGA-AmpB NPs were prepared by well-established nanoprecipitation method and decorated with Phos by thin film hydration method. Physico-chemical characterization of the formulation was done by Zetasizer nano ZS and atomic force microscopy. RESULTS: The optimized formulation (particle size, 157...
February 9, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29381395/differential-expression-of-mirnas-are-associated-with-the-insulin-signaling-pathway-in-preeclampsia-and-gestational-hypertension
#7
Olive P Khaliq, Saravanakumar Murugesan, Jagidesa Moodley, Irene Mackraj
OBJECTIVES: The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients. STUDY DESIGN: Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH were examined from normotensive (n = 32), PE (n = 32) and GH (n = 28) in South African women. Western blot analysis of protein expressions of AKT and PI3K was performed in the placental tissue of all three groups...
January 30, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29372532/n-acetyl-tryptophan-glucoside-natg-protects-j774a-1-murine-macrophages-against-gamma-radiation-induced-cell-death-by-modulating-oxidative-stress
#8
Poonam Malhotra, Ashutosh K Gupta, Darshana Singh, Saurabh Mishra, Shravan K Singh, Raj Kumar
Immune system is amongst the most radiosensitive system to radiation-induced cellular and molecular damage. Present study was focused on the evaluation of radioprotective efficacy of a novel secondary metabolite, N-acetyl tryptophan glucoside (NATG), isolated from a radioresistant bacterium Bacillus sp. INM-1 using murine macrophage J774A.1 cells experimental model. Radioprotective efficacy of NATG against radiation-induced DNA damage and apoptosis was estimated using phosphatidyl-serine-externalization Annexin V-PI and Comet assay analysis...
January 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29341369/structural-factors-affecting-binding-of-platinum-anticancer-agents-with-phospholipids-influence-of-charge-and-phosphate-clamp-formation
#9
Anil Kumar Gorle, Junyong Zhang, Qin Liu, Susan J Berners-Price, Nicholas P Farrell
We report a detailed NMR and DFT study of the interaction of polynuclear platinum anticancer agents (PPCs) with negatively charged phospholipids as a mechanism for their cellular uptake. The reactions of fully 15N-labelled [{trans-PtCl(NH3)2}2(-trans-Pt(NH3)2{NH2(CH2)6NH2}2)]4+ (15N-1, 1,0,1/t,t,t) and the dinuclear [{trans-PtCl(NH3)2}2{-H2N(CH2)6NH2}]2+ (15N-2, 1,1/t,t) with the sodium salt of 1,2-dihexanoyl-sn-glycero-3-phosphate (DHPA) were studied at 298 K, pH ~5.4, by [1H,15N] HSQC 2D NMR spectroscopy...
January 17, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29295901/assessment-of-apoptosis-programmed-cell-death-by-flow-cytometry
#10
Thomas D Duensing, Susan R Watson
A useful feature of therapeutic antibodies is the ability to kill the cells to which they bind. Antibodies are capable of mediating cell killing in a variety of ways. Apoptosis, complement-mediated mechanisms, and antibody-dependent cellular cytotoxicity are all effects that can be assayed to characterize lead antibody candidates. Extensive, multidose characterizations of a series of candidates can be performed in a short amount of time using assays developed for high-throughput flow cytometry systems. Here, we describe a simple multiplexed flow assay performed using Annexin V and propidium iodide that measures an early marker of apoptosis...
January 2, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29225136/in-vitro-apoptotic-mechanism-of-a-novel-synthetic-quinazolinyl-derivative-induces-caspase-dependent-intrinsic-pathway-on-thp-1-leukemia-cell-line
#11
Sridhar Vakamullu, S K Arepalli, L R Velatooru, Venkateswara Rao J, Kavin Kennedy P, Narsaiah B
Several quinazoline derivatives have been found to possess a broad spectrum of biological activities. Previously our research group has synthesized and studied the anti-proliferative effects of N-Decyl-N-(2-Methyl-4-Quinazolinyl) Amine (DMQA). The current study evaluated the cytotoxic and apoptotic properties of DMQA in THP-1 cells. The cytotoxic potential of DMQA was assessed using MTT assay on a panel of cancer cell lines which include HeLa, Mia PaCa-2, A 375, B16-F10, A 549,A 431, U937, THP-1, HL-60 and peripheral blood mononuclear cells (PBMC's)...
January 25, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29157470/therapeutic-potential-of-hiv-nosode-30c-as-evaluated-in-a549-lung-cancer-cells
#12
Anisur Rahman Khuda-Bukhsh, Jesmin Mondal, Rajesh Shah
OBJECTIVES: To examine if HIV nosode in 30c dilution (HIV 30c) has therapeutic potential against lung cancer cells (A549) as compared to WRL-68 normal cells and to elucidate its possible molecular mechanism of action on DNA replication and apoptosis. METHODS: Effects of HIV 30c were thoroughly tested for its possible anticancer potential on A549 cells (lung cancer); WRL-68 normal liver cells served as control. Three doses, one at LD50 and two below LD-50, were used...
November 2017: Homeopathy: the Journal of the Faculty of Homeopathy
https://www.readbyqxmd.com/read/29135861/in-vitro-and-in-vivo-antimelanoma-effect-of-ethyl-ester-cyclohexyl-analog-of-ethylenediamine-dipropanoic-acid
#13
Andjelka M Isakovic, Sasa M Petricevic, Slavica M Ristic, Dusan M Popadic, Tamara K Kravic-Stevovic, Nevena S Zogovic, Jelena M Poljarevic, Tatjana V Zivanovic Radnic, Tibor J Sabo, Aleksandra J Isakovic, Ivanka D Markovic, Vladimir S Trajkovic, Sonja T Misirlic-Dencic
Melanoma, an aggressive skin tumor with high metastatic potential, is associated with high mortality and increasing morbidity. Multiple available chemotherapeutic and immunotherapeutic modalities failed to improve survival in advanced disease, and the search for new agents is ongoing. The aim of this study was to investigate antimelanoma effects of O,O-diethyl-(S,S)-ethylenediamine-N,N'di-2-(3-cyclohexyl) propanoate dihydrochloride (EE), a previously synthesized and characterized organic compound. Mouse melanoma B16 cell viability was assessed using acid phosphatase, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, sulforhodamine B, and lactate dehydrogenase assays...
February 2018: Melanoma Research
https://www.readbyqxmd.com/read/29098899/alteration-of-murine-duodenal-morphology-and-redox-signalling-events-by-reactive-oxygen-species-generated-after-whole-body-%C3%AE-irradiation-and-its-prevention-by-ferulic-acid
#14
Ujjal Das, Aaveri Sengupta, Sushobhan Biswas, Arghya Adhikary, Rakhi Dey Sharma, Anindita Chakraborty, Sanjit Dey
Radiation-induced gastrointestinal syndrome occurs due to the clonogenic loss of crypt cells and villi depopulation, resulting in disruption of the mucosal barrier, bacterial invasion, inflammation, and sepsis. In this study, we investigated the role of ferulic acid (FA) against ionising radiation-induced duodenal injury and subsequent alterations in redox signalling events in wild type male Swiss albino mice. Mice were administered with FA at a dose of 50 mg/kg body weight for 5 consecutive days prior to exposure of 2...
December 2017: Free Radical Research
https://www.readbyqxmd.com/read/29028073/polyphenol-epigallocatechin-3-gallate-alleviates-high-glucose-induced-h9c2-cell-damage-through-pi3k-akt-pathway
#15
Z-M Wang, C-Y Zhong, G-J Zhao
OBJECTIVE: The aim of this work was to study the protective effect of polyphenol epigallocatechin-3-gallate (EGCG) on high glucose-induced oxidative damage of H9C2 cells and to investigate the relationship between this effect and phosphatidyl inositol 3 kinase-serine/threonine kinase (P13K/Akt) signal transduction pathway. MATERIALS AND METHODS: H9C2 cells were used as objects of study, 350 mM glucose serum-free medium was used as the high glucose molding condition, and LY294002 (10 μM) was used as the PI3K/Akt inhibitor...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28936738/vascular-calcification-regulation-by-exosomes-in-the-vascular-wall
#16
REVIEW
Marcel Liberman, Luciana Cavalheiro Marti
Vascular calcification is a tightly regulated process that increases during ageing and occurs mainly in patients with diabetes and chronic renal failure. Exosomes are small membrane vesicles that are synthesized in a particular population of endosomes, also called multivesicular bodies, by inside budding into the lumen of the compartment. After fusion of exosome with the plasma membrane, these internal vesicles are secreted. Exosomes have a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28821470/design-of-the-drepagreffe-trial-a-prospective-controlled-multicenter-study-evaluating-the-benefit-of-genoidentical-hematopoietic-stem-cell-transplantation-over-chronic-transfusion-in-sickle-cell-anemia-children-detected-to-be-at-risk-of-stroke-by-transcranial
#17
Sylvie Chevret, Suzanne Verlhac, Elisabeth Ducros-Miralles, Jean-Hugues Dalle, Regis Peffault de Latour, Mariane de Montalembert, Malika Benkerrou, Corinne Pondarré, Isabelle Thuret, Corinne Guitton, Emmanuelle Lesprit, Maryse Etienne-Julan, Gisèle Elana, Jean-Pierre Vannier, Patrick Lutz, Bénédicte Neven, Claire Galambrun, Catherine Paillard, Camille Runel, Charlotte Jubert, Cécile Arnaud, Annie Kamdem, Valentine Brousse, Florence Missud, Marie Petras, Lydia Doumdo-Divialle, Claire Berger, Françoise Fréard, Olivier Taieb, Elise Drain, Monique Elmaleh, Manuela Vasile, Yacine Khelif, Myriam Bernaudin, Philippe Chadebech, France Pirenne, Gérard Socié, Françoise Bernaudin
BACKGROUND: Children with sickle cell anemia (SCA) have an 11% risk of stroke by the age of 18. Chronic transfusion applied in patients detected to be at risk by transcranial Doppler allows a significant reduction of stroke risk. However, chronic transfusion exposes to several adverse events, including alloimmunization and iron overload, and is not curative. Hematopoietic stem cell transplantation allows termination of the transfusion program, but its benefit has not been demonstrated...
November 2017: Contemporary Clinical Trials
https://www.readbyqxmd.com/read/28802974/characteristics-of-thrombin-generation-in-a-fulminant-porcine-sepsis-model
#18
Judit Tóth, Ildikó Beke Debreceni, Ádám Deák, Katalin Pető, Mariann Berhés, Endre Hajdú, Judit Szabó, Norbert Németh, Béla Fülesdi, János Kappelmayer
INTRODUCTION: The activation of blood coagulation has been demonstrated in most cases of sepsis, however previous studies in humans could not detect hypercoagulability with global hemostasis assays. In a fulminant porcine sepsis model we analysed coagulation screening tests and thrombin generation to evaluate hemostatic alterations. MATERIALS AND METHODS: Live Escherichia coli bacteria were inoculated to female pigs and prothrombin time, activated partial thromboplastin time, thrombin time and fibrinogen were measured by coagulometry...
October 2017: Thrombosis Research
https://www.readbyqxmd.com/read/28739368/andrographolide-induces-oxidative-stress-dependent-cell-death-in-unicellular-protozoan-parasite-trypanosoma-brucei
#19
Malabika Banerjee, Debaprasad Parai, Pranab Dhar, Manab Roy, Rajib Barik, Subrata Chattopadhyay, Samir Kumar Mukherjee
African sleeping sickness is a parasitic disease in humans and livestock caused by Trypanosoma brucei throughout sub-Saharan Africa. Absence of appropriate vaccines and prevalence of drug resistance proclaim that a new way of therapeutic interventions is essential against African trypanosomiasis. In the present study, we have looked into the effect of andrographolide (andro), a diterpenoid lactone from Andrographis paiculata on Trypanosoma brucei PRA 380. Although andro has been recognized as a promosing anti-cancer drug, its usefulness against Trypanosoma spp remained unexplored...
December 2017: Acta Tropica
https://www.readbyqxmd.com/read/28730954/evaluation-of-in-vitro-anti-psoriatic-activity-of-a-novel-polyherbal-formulation-by-multiparametric-analysis
#20
Nadav Shraibom, Alka Madaan, Vidushi Joshi, Ritu Verma, Anika Chaudhary, Gautam Mishra, Anshumali Awasthi, Anu Taneja Singh, Manu Jaggi
BACKGROUND: We have developed a novel aqueous polyherbal formulation (SIRB-001) consisting of 3 herbs; Rheum palmatum L., Lonicera Japonica and Rehmannia glutinosa Libosch in the ratio 1:1:3. SIRB-001 has demonstrated efficacious effects in psoriasis patients. OBJECTIVE: This study was aimed at scientifically evaluating the in vitro antipsoriatic activity of SIRB-001. METHOD: The in vitro anti-psoriatic properties of SIRB-001 were assessed in human keratinocyte cell line; HaCaT...
2017: Anti-inflammatory & Anti-allergy Agents in Medicinal Chemistry
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