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https://www.readbyqxmd.com/read/29899957/stapled-peptides-as-a-new-technology-to-investigate-protein-protein-interactions-in-human-platelets
#1
Jessica Iegre, Niaz S Ahmed, Josephine S Gaynord, Yuteng Wu, Kara M Herlihy, Yaw Sing Tan, Maria E Lopes-Pires, Rupam Jha, Yu Heng Lau, Hannah F Sore, Chandra Verma, Daniel H O' Donovan, Nicholas Pugh, David R Spring
Platelets are blood cells with numerous crucial pathophysiological roles in hemostasis, cardiovascular thrombotic events and cancer metastasis. Platelet activation requires the engagement of intracellular signalling pathways that involve protein-protein interactions (PPIs). A better understanding of these pathways is therefore crucial for the development of selective anti-platelet drugs. New strategies for studying PPIs in human platelets are required to overcome limitations associated with conventional platelet research methods...
May 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29885293/polyhexamethylene-biguanide-and-chloroquine-induce-programmed-cell-death-in-acanthamoeba-castellanii
#2
Eun-Kyung Moon, Hyun-Seo Choi, Hyun-Hee Kong, Fu-Shi Quan
Several chemotherapeutic drugs have been described as amoebicidal agents acting against Acanthamoeba trophozoites and cysts. However, the underlying mechanism of action is poorly characterized. Here, we describe programmed cell death (PCD) in A. castellanii induced by polyhexamethylene biguanide (PHMB) and chloroquine. We used four types of amoebicidal agents including 0.02% PHMB, 0.02% chlorhexidine digluconate, 100 μM chloroquine, and 100 μM 2,6-dichlorobenzonitrile to kill Acanthamoeba trophozoites and cysts...
June 6, 2018: Experimental Parasitology
https://www.readbyqxmd.com/read/29843771/neutrophil-extracellular-traps-induce-aggregation-of-washed-human-platelets-independently-of-extracellular-dna-and-histones
#3
Omar Elaskalani, Norbaini Binti Abdol Razak, Pat Metharom
BACKGROUND: The release of neutrophil extracellular traps (NETs), a mesh of DNA, histones and neutrophil proteases from neutrophils, was first demonstrated as a host defence against pathogens. Recently it became clear that NETs are also released in pathological conditions. NETs released in the blood can activate thrombosis and initiate a cascade of platelet responses. However, it is not well understood if these responses are mediated through direct or indirect interactions. We investigated whether cell-free NETs can induce aggregation of washed human platelets in vitro and the contribution of NET-derived extracellular DNA and histones to platelet activation response...
May 29, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29742069/a-simple-robust-method-of-synthesis-of-copper-silver-core-shell-nano-particle-evaluation-of-its-structural-and-chemical-properties-with-anticancer-potency
#4
Milon Banik, Mousumi Patra, Debanjan Dutta, Riya Mukherjee, Tarakdas Basu
A simple method of synthesis of stable bimetallic copper-silver nanoparticle (Cu@Ag NP) was developed by successive reduction of Cu(NO3)2 and AgNO3, using hydrazine hydrate as the reducing agent and gelatine and Poly-vinyl pyrrolidone (PVP) as the capping agents. The round-shaped particles were of core-shell structure with a core of Cu0 atoms surrounded by a shell of Ag0 atoms. The size and the mol. wt. of the NPs were (100 ± 10) nm and (820 ± 157) Kd respectively; the particles were crystalline in nature and 90% of the precursors Cu(NO3)2 and AgNO3 were converted to the NPs...
May 9, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29665555/hesperidin-promotes-programmed-cell-death-by-downregulation-of-nongenomic-estrogen-receptor-signalling-pathway-in-endometrial-cancer-cells
#5
Z B Cincin, B Kiran, Y Baran, B Cakmakoglu
Endometrial carcinoma (EC) is the most common malignant gynecologic tumor in women. EC is thought to be caused by increasing estrogen levels relative to progesterone in the body. Hesperidin (Hsd), a biologically active flavonoid, could be extracted from Citrus species. It has been recently shown that Hsd could exert anticarcinogenic properties in different cancer types. However, the effects of Hsd and its molecular mechanisms on EC remain unclear. In this study, the antiproliferative, apoptotic and genomic effects of Hsd in EC and its underlying mechanisms were identified...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29622550/14-3-3-proteins-in-platelet-biology-and-glycoprotein-ib-ix-signaling
#6
REVIEW
Yunfeng Chen, Zaverio M Ruggeri, Xiaoping Du
Members of the 14-3-3 family of proteins function as adapters/modulators that recognize phosphoserine/phosphothreonine-based binding motifs in many intracellular proteins and play fundamental roles in signal transduction pathways of eukaryotic cells. In platelets, 14-3-3 plays a wide range of regulatory roles in phosphorylation-dependent signaling pathways, including G-protein signaling, cAMP signaling, agonist-induced phosphatidylserine exposure, and regulation of mitochondrial function. In particular, 14-3-3 interacts with several phosphoserine-dependent binding sites in the major platelet adhesion receptor, the glycoprotein Ib-IX complex (GPIb-IX), regulating its interaction with von Willebrand factor (VWF) and mediating VWF/GPIb-IX-dependent mechanosignal transduction, leading to platelet activation...
May 31, 2018: Blood
https://www.readbyqxmd.com/read/29486557/dual-functions-of-lip6-and-its-regulation-of-lipid-metabolism-in-the-oleaginous-fungus-mucor-circinelloides
#7
Xinyi Zan, Xin Tang, Linfang Chu, Yuanda Song
Although multiple roles of lipases have been reported in yeasts and microalgae, the functions of lipases have not been studied in oleaginous filamentous fungi. Lipase Lip6 has been reported in the oleaginous filamentous fungus Mucor circinelloides with the consensus lipase motif GXSXG and the typical acyltransferase motif of H-(X)4 -D. To demonstrate that Lip6 might play dual roles as a lipase and an acyltransferase, we performed site-directed mutagenesis in the lipase motif and the acyltransferase motif of Lip6...
March 21, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29474406/the-role-of-the-folate-pathway-in-pancreatic-cancer-risk
#8
Shirisha Chittiboyina, Zhongxue Chen, E Gabriela Chiorean, Lisa M Kamendulis, Barbara A Hocevar
BACKGROUND: Pancreatic cancer is the third leading cause of cancer related deaths in the United States. Several dietary factors have been identified that modify pancreatic cancer risk, including low folate levels. In addition to nutrition and lifestyle determinants, folate status may be influenced by genetic factors such as single nucleotide polymorphisms (SNPs). In the present study, we investigated the association between folate levels, genetic polymorphisms in genes of the folate pathway, and pancreatic cancer...
2018: PloS One
https://www.readbyqxmd.com/read/29462602/6-shogaol-induces-caspase-independent-paraptosis-in-cancer-cells-via-proteasomal-inhibition
#9
Divya Nedungadi, Anupama Binoy, Nanjan Pandurangan, Sanjay Pal, Bipin G Nair, Nandita Mishra
An α, β-unsaturated carbonyl compound of ginger, 6-Shogaol (6S), induced extensive cytoplasmic vacuolation and cell death in breast cancer cell (MDA-MB-231) and non-small lung cancer (A549) cells. In the presence of autophagic inhibitors the cells continued to exhibit cytoplasmic vacuolation and cell death clearly distinguishing it from the classic autophagic process. 6S induced death did not exhibit the characteristic apoptotic features like caspase cleavage, phosphatidyl serine exposure and DNA fragmentation...
March 15, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29427248/fabrication-of-3-o-sn-phosphatidyl-l-serine-anchored-plga-nanoparticle-bearing-amphotericin-b-for-macrophage-targeting
#10
Pankaj K Singh, Anil K Jaiswal, Vivek K Pawar, Kavit Raval, Animesh Kumar, Himangsu K Bora, Anuradha Dube, Manish K Chourasia
PURPOSE: To fabricate, characterize and evaluate 3-O-sn-Phosphatidyl-L-serine (PhoS) anchored PLGA nanoparticles for macrophage targeted therapeutic intervention of VL. MATERIALS AND METHODS: PLGA-AmpB NPs were prepared by well-established nanoprecipitation method and decorated with Phos by thin film hydration method. Physico-chemical characterization of the formulation was done by Zetasizer nano ZS and atomic force microscopy. RESULTS: The optimized formulation (particle size, 157...
February 9, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29381395/differential-expression-of-mirnas-are-associated-with-the-insulin-signaling-pathway-in-preeclampsia-and-gestational-hypertension
#11
Olive P Khaliq, Saravanakumar Murugesan, Jagidesa Moodley, Irene Mackraj
OBJECTIVES: The objective of this study was to determine microRNAs (miRNAs) expression levels in placental tissue and serum samples from preeclampsia (PE) and gestational hypertensive (GH) patients. STUDY DESIGN: Using a targeted qPCR method, the selected miRNAs putatively involved in the PE and GH were examined from normotensive (n = 32), PE (n = 32) and GH (n = 28) in South African women. Western blot analysis of protein expressions of AKT and PI3K was performed in the placental tissue of all three groups...
January 30, 2018: Clinical and Experimental Hypertension: CHE
https://www.readbyqxmd.com/read/29372532/n-acetyl-tryptophan-glucoside-natg-protects-j774a-1-murine-macrophages-against-gamma-radiation-induced-cell-death-by-modulating-oxidative-stress
#12
Poonam Malhotra, Ashutosh K Gupta, Darshana Singh, Saurabh Mishra, Shravan K Singh, Raj Kumar
Immune system is amongst the most radiosensitive system to radiation-induced cellular and molecular damage. Present study was focused on the evaluation of radioprotective efficacy of a novel secondary metabolite, N-acetyl tryptophan glucoside (NATG), isolated from a radioresistant bacterium Bacillus sp. INM-1 using murine macrophage J774A.1 cells experimental model. Radioprotective efficacy of NATG against radiation-induced DNA damage and apoptosis was estimated using phosphatidyl-serine-externalization Annexin V-PI and Comet assay analysis...
January 25, 2018: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29341369/structural-factors-affecting-binding-of-platinum-anticancer-agents-with-phospholipids-influence-of-charge-and-phosphate-clamp-formation
#13
Anil Kumar Gorle, Junyong Zhang, Qin Liu, Susan J Berners-Price, Nicholas P Farrell
We report a detailed NMR and DFT study of the interaction of polynuclear platinum anticancer agents (PPCs) with negatively charged phospholipids as a mechanism for their cellular uptake. The reactions of fully 15 N-labelled [{trans-PtCl(NH3 )2 }2 (μ-trans-Pt(NH3 )2 {NH2 (CH2 )6 NH2 }2 )]4+ (15 N-1, 1,0,1/t,t,t) and the dinuclear [{trans-PtCl(NH3 )2 }2 {μ-H2 N(CH2 )6 NH2 }]2+ (15 N-2, 1,1/t,t) with the sodium salt of 1,2-dihexanoyl-sn-glycero-3-phosphate (DHPA) were studied at 298 K, pH ≈5.4, by [1 H,15 N] HSQC 2D NMR spectroscopy...
March 26, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29295901/assessment-of-apoptosis-programmed-cell-death-by-flow-cytometry
#14
Thomas D Duensing, Susan R Watson
A useful feature of therapeutic antibodies is the ability to kill the cells to which they bind. Antibodies are capable of mediating cell killing in a variety of ways. Apoptosis, complement-mediated mechanisms, and antibody-dependent cellular cytotoxicity are all effects that can be assayed to characterize lead antibody candidates. Extensive, multidose characterizations of a series of candidates can be performed in a short amount of time using assays developed for high-throughput flow cytometry systems. Here, we describe a simple multiplexed flow assay performed using Annexin V and propidium iodide that measures an early marker of apoptosis...
January 2, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29225136/in-vitro-apoptotic-mechanism-of-a-novel-synthetic-quinazolinyl-derivative-induces-caspase-dependent-intrinsic-pathway-on-thp-1-leukemia-cell-line
#15
Sridhar Vakamullu, S K Arepalli, L R Velatooru, Venkateswara Rao J, Kavin Kennedy P, Narsaiah B
Several quinazoline derivatives have been found to possess a broad spectrum of biological activities. Previously our research group has synthesized and studied the anti-proliferative effects of N-Decyl-N-(2-Methyl-4-Quinazolinyl) Amine (DMQA). The current study evaluated the cytotoxic and apoptotic properties of DMQA in THP-1 cells. The cytotoxic potential of DMQA was assessed using MTT assay on a panel of cancer cell lines which include HeLa, Mia PaCa-2, A 375, B16-F10, A 549,A 431, U937, THP-1, HL-60 and peripheral blood mononuclear cells (PBMC's)...
January 25, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29157470/therapeutic-potential-of-hiv-nosode-30c-as-evaluated-in-a549-lung-cancer-cells
#16
Anisur Rahman Khuda-Bukhsh, Jesmin Mondal, Rajesh Shah
OBJECTIVES: To examine if HIV nosode in 30c dilution (HIV 30c) has therapeutic potential against lung cancer cells (A549) as compared to WRL-68 normal cells and to elucidate its possible molecular mechanism of action on DNA replication and apoptosis. METHODS: Effects of HIV 30c were thoroughly tested for its possible anticancer potential on A549 cells (lung cancer); WRL-68 normal liver cells served as control. Three doses, one at LD50 and two below LD-50, were used...
November 2017: Homeopathy: the Journal of the Faculty of Homeopathy
https://www.readbyqxmd.com/read/29135861/in-vitro-and-in-vivo-antimelanoma-effect-of-ethyl-ester-cyclohexyl-analog-of-ethylenediamine-dipropanoic-acid
#17
Andjelka M Isakovic, Sasa M Petricevic, Slavica M Ristic, Dusan M Popadic, Tamara K Kravic-Stevovic, Nevena S Zogovic, Jelena M Poljarevic, Tatjana V Zivanovic Radnic, Tibor J Sabo, Aleksandra J Isakovic, Ivanka D Markovic, Vladimir S Trajkovic, Sonja T Misirlic-Dencic
Melanoma, an aggressive skin tumor with high metastatic potential, is associated with high mortality and increasing morbidity. Multiple available chemotherapeutic and immunotherapeutic modalities failed to improve survival in advanced disease, and the search for new agents is ongoing. The aim of this study was to investigate antimelanoma effects of O,O-diethyl-(S,S)-ethylenediamine-N,N'di-2-(3-cyclohexyl) propanoate dihydrochloride (EE), a previously synthesized and characterized organic compound. Mouse melanoma B16 cell viability was assessed using acid phosphatase, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, sulforhodamine B, and lactate dehydrogenase assays...
February 2018: Melanoma Research
https://www.readbyqxmd.com/read/29098899/alteration-of-murine-duodenal-morphology-and-redox-signalling-events-by-reactive-oxygen-species-generated-after-whole-body-%C3%AE-irradiation-and-its-prevention-by-ferulic-acid
#18
Ujjal Das, Aaveri Sengupta, Sushobhan Biswas, Arghya Adhikary, Rakhi Dey Sharma, Anindita Chakraborty, Sanjit Dey
Radiation-induced gastrointestinal syndrome occurs due to the clonogenic loss of crypt cells and villi depopulation, resulting in disruption of the mucosal barrier, bacterial invasion, inflammation, and sepsis. In this study, we investigated the role of ferulic acid (FA) against ionising radiation-induced duodenal injury and subsequent alterations in redox signalling events in wild type male Swiss albino mice. Mice were administered with FA at a dose of 50 mg/kg body weight for 5 consecutive days prior to exposure of 2...
December 2017: Free Radical Research
https://www.readbyqxmd.com/read/29028073/polyphenol-epigallocatechin-3-gallate-alleviates-high-glucose-induced-h9c2-cell-damage-through-pi3k-akt-pathway
#19
Z-M Wang, C-Y Zhong, G-J Zhao
OBJECTIVE: The aim of this work was to study the protective effect of polyphenol epigallocatechin-3-gallate (EGCG) on high glucose-induced oxidative damage of H9C2 cells and to investigate the relationship between this effect and phosphatidyl inositol 3 kinase-serine/threonine kinase (P13K/Akt) signal transduction pathway. MATERIALS AND METHODS: H9C2 cells were used as objects of study, 350 mM glucose serum-free medium was used as the high glucose molding condition, and LY294002 (10 μM) was used as the PI3K/Akt inhibitor...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28936738/vascular-calcification-regulation-by-exosomes-in-the-vascular-wall
#20
REVIEW
Marcel Liberman, Luciana Cavalheiro Marti
Vascular calcification is a tightly regulated process that increases during ageing and occurs mainly in patients with diabetes and chronic renal failure. Exosomes are small membrane vesicles that are synthesized in a particular population of endosomes, also called multivesicular bodies, by inside budding into the lumen of the compartment. After fusion of exosome with the plasma membrane, these internal vesicles are secreted. Exosomes have a defined set of membrane and cytosolic proteins. The physiological function of exosomes is still a matter of debate...
2017: Advances in Experimental Medicine and Biology
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