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https://www.readbyqxmd.com/read/28322991/thyroid-hormone-induce-a-p53-dependent-dna-damage-through-pi3k-akt-activation-in-sperm
#1
Hong Xian, Fang Wang, Wending Teng, Dong Yang, Ming Zhang
Accumulating evidences showed that thyroid hormone was participated in the functioning of the reproductive system, and an elevated level of thyroid hormones had a negative impact on reproductive system. However, the molecular basis for this observation still remains to be fully understood. Here, we show that l-Thyroxine significantly impaired human sperm motility. The molecular basis showed that thyroxine receptor stimulation triggers Phosphatidyl Inositol 3-kinase (PI3K)/Akt signaling activation leading to the E3 ligase MDM2 phosphorylation at serine 166, which directly interacted with p53 for degradation...
June 5, 2017: Gene
https://www.readbyqxmd.com/read/28306533/ros-mediated-induction-of-apoptosis-by-benzoquinone-embelin-in-human-colon-adenocarcinoma-cells-ht-29
#2
Kodandaram Sumalatha, Mohan Gowda, Sreepriya Meenakshisundaram
Background Embelin is a benzoquinone reported to possess anticancer activity in several in vivo and in vitro models of carcinogenesis, especially hematopoietic and prostate malignancy. A detailed investigation on the influence of embelin on epithelial malignancy model system, especially colon adenocarcinoma, is lacking. The objective of the current study is to investigate the antiproliferative, antiinvasive and proapoptotic potential of embelin on colon adenocarcinoma cell line HT-29. Methods The effect of embelin (35 µg/mL for 24 h) on cell proliferation was assessed by Sulforhodamine B assay and bromodeoxyuridine incorporation test, antiinvasive effect by Boyden chamber assay and scratch assay...
March 17, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28286882/a-study-to-investigate-the-role-of-gulp-ced-6-gene-in-eat-me-signaling-in-cellular-efferocytosis-and-immunosurveillance
#3
H Banerjee, B Bazemore, A Barfield, D Crummity, C Krauss, G Payne, J Rousch, V Manglik
In this report, investigations were done to study human GULP/ CED 6 genes role in presenting cancer cells to scavenger cells. CED 6 SiRNA was used to knock out the gene in Astrocytoma (HTB-12) cell lines to study its effects on expression of various "eat me" signals on these cells including Phosphatidyl serine (PtdSer) expression, nitric oxide (NO) signaling and Leukotrine B4 (LTB4) expression and Caspase 3 activation. Investigations were done by fluorescence microscopy techniques, ELISA assay and colorimetric assays using a standard microplate reader and spectrophotometer...
December 2016: Immunological Disorders & Immunotherapy
https://www.readbyqxmd.com/read/28286004/computational-modeling-reveals-that-signaling-lipids-modulate-the-orientation-of-k-ras4a-at-the-membrane-reflecting-protein-topology
#4
Zhen-Lu Li, Matthias Buck
The structural, dynamical, and functional characterization of the small GTPase K-Ras has become a research area of intense focus due to its high occurrence in human cancers. Ras proteins are only fully functional when they interact with the plasma membrane. Here we present all-atom molecular dynamics simulations (totaling 5.8 μs) to investigate the K-Ras4A protein at membranes that contain anionic lipids (phosphatidyl serine or phosphatidylinositol bisphosphate). We find that similarly to the homologous and highly studied K-Ras4B, K-Ras4A prefers a few distinct orientations at the membrane...
April 4, 2017: Structure
https://www.readbyqxmd.com/read/28273904/methionine-aminopeptidase-2-is-a-key-regulator-of-apoptotic-like-cell-death-in-leishmania-donovani
#5
Ritesh Kumar, Kartikeya Tiwari, Vikash Kumar Dubey
We investigate the role of methionine aminopeptidase 2 (MAP2) in miltefosine induced programmed cell death (PCD) in promastigote form of L. donovani. We report that TNP-470, an inhibitor of MAP2, inhibits programmed cell death in miltefosine treated promastigotes. It inhibits the biochemical features of metazoan apoptosis, including caspase3/7 protease like activity, oligonucleosomal DNA fragmentation, collapse of mitochondrial transmembrane potential, and increase in cytosolic pool of calcium ions but did not prevent the cell death and phosphatidyl serine externalization...
December 2017: Scientific Reports
https://www.readbyqxmd.com/read/28123531/goniothalamin-induces-mitochondria-mediated-apoptosis-associated-with-endoplasmic-reticulum-stress-induced-activation-of-jnk-in-hela-cells
#6
Thanet Sophonnithiprasert, Wilawan Mahabusarakam, Yukio Nakamura, Ramida Watanapokasin
Goniothalamin, a natural occurring styryl-lactone isolated from Goniothalamus macrophyllus (Blume) Hook. f. & Thomson var. macrophyllus, can trigger cancer cell death in various types of cancer cell. The present study focused on elucidation of the mitochondria-mediated apoptosis associated with endoplasmic reticulum (ER) stress-induced activation of c-Jun NH2-terminal kinase (JNK) by goniothalamin in HeLa cervical cancer cells. Cell viability was determined using an MTT assay, and DNA condensation and loss of mitochondrial membrane potential were determined using Hoechst 33342 and JC-1 staining, respectively...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/27896225/the-urokinase-urokinase-receptor-system-in-mast-cells-effects-of-its-functional-interaction-with-fmlf-receptors
#7
Francesca Wanda Rossi, Nella Prevete, Felice Rivellese, Filomena Napolitano, Nunzia Montuori, Loredana Postiglione, Carmine Selleri, Amato de Paulis
Mast cell and basophils express the high affinity receptor for IgE (FcɛRI) and are primary effector cells of allergic disorders. The urokinase (uPA)-mediated plasminogen activation system is involved in physiological and pathological events based on cell migration and tissue remodelling, such as inflammation, wound healing, angiogenesis and metastasis. uPA is a serine protease that binds uPAR, a high affinity glycosyl-phosphatidyl-inositol (GPI)-anchored receptor. uPAR focuses uPA activity at the cell surface and activates intracellular signaling through lateral interactions with integrins, receptor tyrosine kinases and the G-protein-coupled family of fMLF chemotaxis receptors (FPRs)...
November 2016: Translational Medicine @ UniSa
https://www.readbyqxmd.com/read/27869259/the-effect-of-top-layer-chemistry-on-the-formation-of-supported-lipid-bilayers-on-polyelectrolyte-multilayers-primary-versus-quaternary-amines
#8
E Diamanti, P Andreozzi, R Anguiano, L Yate, D Gregurec, N Politakos, R F Ziolo, E Donath, S E Moya
The influence of the surface chemistry of polyelectrolyte multilayers (PEMs) on the formation of lipid bilayers is studied here for PEMs with either polyallylamine hydrochloride (PAH) or polydiallyldimethylammonium chloride (PDADMAC) as a polycation as a top layer, and polystyrene sulfonate (PSS) as a polyanion. Small unilamellar vesicles (SUVs) composed of phosphatidyl choline and phosphatidyl serine at a 50 : 50 molar ratio are deposited on top of the PEM films. The assembly of the SUVs into bilayers is studied via a quartz crystal microbalance with dissipation (QCM-D) and fluorescence recovery after photobleaching (FRAP)...
November 30, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27864176/insights-into-the-biosynthesis-of-duramycin
#9
Liujie Huo, Ayşe Ökesli, Ming Zhao, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are characterized by the thioether cross-linked bisamino acids lanthionine (Lan) and methyllanthionine (MeLan). Duramycin contains 19 amino acids, including one Lan and two MeLans, an unusual lysinoalanine (Lal) bridge formed from the ε-amino group of lysine 19 and a serine residue at position 6, and an erythro-3-hydroxy-l-aspartic acid at position 15. These modifications are important for the interactions of duramycin with its biological target, phosphatidylethanolamine (PE)...
February 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27836661/phosphorylation-of-poly-rc-binding-protein-1-pcbp1-contributes-to-stabilization-of-mu-opioid-receptor-mor-mrna-via-interaction-with-au-rich-element-rna-binding-protein-1-auf1-and-poly-a-binding-protein-pabp
#10
Cheol Kyu Hwang, Yadav Wagley, Ping-Yee Law, Li-Na Wei, Horace H Loh
Gene regulation at the post-transcriptional level is frequently based on cis- and trans-acting factors on target mRNAs. We found a C-rich element (CRE) in mu-opioid receptor (MOR) 3'-untranslated region (UTR) to which poly (rC) binding protein 1 (PCBP1) binds, resulting in MOR mRNA stabilization. RNA immunoprecipitation and RNA EMSA revealed the formation of PCBP1-RNA complexes at the element. Knockdown of PCBP1 decreased MOR mRNA half-life and protein expression. Stimulation by forskolin increased cytoplasmic localization of PCBP1 and PCBP1/MOR 3'-UTR interactions via increased serine phosphorylation that was blocked by protein kinase A (PKA) or (phosphatidyl inositol-3) PI3-kinase inhibitors...
January 20, 2017: Gene
https://www.readbyqxmd.com/read/27824861/identification-of-biomarkers-of-impaired-sensory-profiles-among-autistic-patients
#11
Afaf El-Ansary, Wail M Hassan, Hanan Qasem, Undurti N Das
BACKGROUND: Autism is a neurodevelopmental disorder that displays significant heterogeneity. Comparison of subgroups within autism, and analyses of selected biomarkers as measure of the variation of the severity of autistic features such as cognitive dysfunction, social interaction impairment, and sensory abnormalities might help in understanding the pathophysiology of autism. METHODS AND PARTICIPANTS: In this study, two sets of biomarkers were selected. The first included 7, while the second included 6 biomarkers...
2016: PloS One
https://www.readbyqxmd.com/read/27712999/evaluation-of-the-cytotoxic-apoptosis-inducing-activity-and-molecular-docking-of-spiroquinazolinone-benzamide-derivatives-in-mcf-7-breast-cancer-cells
#12
Majid Mahdavi, Malihe Mohseni Lavi, Reza Yekta, Mohammad Amin Moosavi, Mahnaz Nobarani, Saeed Balalaei, Sanam Arami, Mohammad Reza Rashidi
Previous studies have suggested that quinazolinone derivatives are potent apoptosis-inducing agents in various cancer cell lines. In the present study, we have investigated cytotoxic, apoptosis induction, and molecular docking activities of the spiroquinazolinone benzamide derivatives family on MCF-7 human breast cancer cells. The MTT cytotoxicity assays and docking studies showed that 4t-CHQB was the most active compound among the prepared spiroquinazolinone benzamide compounds with IC50 of 50 ± 1.2 μM and was selected for further assessments...
December 25, 2016: Chemico-biological Interactions
https://www.readbyqxmd.com/read/27624766/total-%C3%AE-synuclein-levels-in-human-blood-cells-csf-and-saliva-determined-by-a-lipid-elisa
#13
Suaad Abd-Elhadi, Misericordia Basora, Dolores Vilas, Eduardo Tolosa, Ronit Sharon
The validity of α-synuclein (α-Syn) as a biomarker for Parkinson's disease (PD) is still under investigation. Conventional methods for capture and quantitation of α-Syn protein in human samples are primarily based on anti-α-Syn antibodies. Specific and competent antibodies were raised against α-Syn. However, capture by anti-α-Syn antibodies may be limited to specific epitope recognition, attributed to protein structure or post-translational modifications. Hence, antibody-based methods for α-Syn capture raise a concern regarding their efficacy to detect the intracellular, unfolded α-Syn pool...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27502061/detection-of-myocarditis-with-molecular-echo-imaging-another-potential-application-for-the-phosphatidyl-serine-microbubble
#14
EDITORIAL
Thomas R Porter
No abstract text is available yet for this article.
August 2016: Circulation. Cardiovascular Imaging
https://www.readbyqxmd.com/read/27473012/identification-of-a-secretory-phospholipase-a2-from-papaver-somniferum-l-that-transforms-membrane-phospholipids
#15
Veronika Jablonická, Johanna Mansfeld, Ingo Heilmann, Marek Obložinský, Mareike Heilmann
The full-length sequence of a new secretory phospholipase A2 was identified in opium poppy seedlings (Papaver somniferum L.). The cDNA of poppy phospholipase A2, denoted as pspla2, encodes a protein of 159 amino acids with a 31 amino acid long signal peptide at the N-terminus. PsPLA2 contains a PLA2 signature domain (PA2c), including the Ca(2+)-binding loop (YGKYCGxxxxGC) and the catalytic site motif (DACCxxHDxC) with the conserved catalytic histidine and the calcium-coordinating aspartate residues. The aspartate of the His/Asp dyad playing an important role in animal sPLA2 catalysis is substituted by a serine residue...
September 2016: Phytochemistry
https://www.readbyqxmd.com/read/27449044/sperm-associated-antigen-9-spag9-promotes-the-survival-and-tumor-growth-of-triple-negative-breast-cancer-cells
#16
Nirmala Jagadish, Namita Gupta, Sumit Agarwal, Deepak Parashar, Aditi Sharma, Rukhsar Fatima, Amos Prashant Topno, Vikash Kumar, Anil Suri
Recently, we demonstrated the association of sperm-associated antigen 9 (SPAG9) expression with breast cancer. Among breast cancer, 15 % of the cancers are diagnosed as triple-negative breast cancers (TNBC) based on hormone receptor status and represent an important clinical challenge because of lack of effective available targeted therapy. Therefore, in the present investigation, plasmid-based small hairpin (small hairpin RNA (shRNA)) approach was used to ablate SPAG9 in aggressive breast cancer cell line model (MDA-MB-231) in order to understand the role of SPAG9 at molecular level in apoptosis, cell cycle, and epithelial-to-mesenchymal transition (EMT) signaling...
October 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27431587/flat-oyster-follows-the-apoptosis-pathway-to-defend-against-the-protozoan-parasite-bonamia-ostreae
#17
Ophélie Gervais, Bruno Chollet, Tristan Renault, Isabelle Arzul
The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O...
September 2016: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/27316541/synthetic-sulfoglycolipids-targeting-the-serine-threonine-protein-kinase-akt
#18
Barbara Costa, Milind Dangate, Maria Vetro, Giulia Donvito, Luca Gabrielli, Loredana Amigoni, Giuliana Cassinelli, Cinzia Lanzi, Michela Ceriani, Luca De Gioia, Giulia Filippi, Laura Cipolla, Nadia Zaffaroni, Paola Perego, Diego Colombo
The serine-threonine protein kinase Akt, also known as protein kinase B, is a key component of the phosphoinositide 3-kinase (PI3K)-Akt-mTOR axis. Deregulated activation of this pathway is frequent in human tumors and Akt-dependent signaling appears to be critical in cell survival. PI3K activation generates 3-phosphorylated phosphatidylinositols that bind Akt pleckstrin homology (PH) domain. The blockage of Akt PH domain/phosphoinositides interaction represents a promising approach to interfere with the oncogenic potential of over-activated Akt...
August 15, 2016: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/27160815/the-fungicide-mancozeb-induces-metacaspase-dependent-apoptotic-cell-death-in-saccharomyces-cerevisiae-by4741
#19
F J Scariot, L M Jahn, J P Maianti, A P L Delamare, S Echeverrigaray
Mancozeb (MZ), a mixture of ethylene-bis-dithiocarbamate manganese and zinc salts, is one of the most widely used fungicides in agriculture. Toxicologic studies in mammals and mammalian cells indicate that this fungicide can cause neurological and cytological disorders, putatively associated with pro-oxidant and apoptotic effects. Yeast adaptation to sub-inhibitory concentrations of MZ has been correlated with oxidative response, proteins degradation, and energy metabolism, and its main effect on yeast has been attributed to its high reactivity with thiol groups in proteins...
July 2016: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27084510/cytotoxicity-and-cell-cycle-arrest-induced-by-andrographolide-lead-to-programmed-cell-death-of-mda-mb-231-breast-cancer-cell-line
#20
Malabika Banerjee, Subrata Chattopadhyay, Tathagata Choudhuri, Rammohan Bera, Sanjay Kumar, Biswajit Chakraborty, Samir Kumar Mukherjee
BACKGROUND: Breast cancer is considered as an increasing major life-threatening concern among the malignancies encountered globally in females. Traditional therapy is far from satisfactory due to drug resistance and various side effects, thus a search for complementary/alternative medicines from natural sources with lesser side effects is being emphasized. Andrographis paniculata, an oriental, traditional medicinal herb commonly available in Asian countries, has a long history of treating a variety of diseases, such as respiratory infection, fever, bacterial dysentery, diarrhea, inflammation etc...
April 16, 2016: Journal of Biomedical Science
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