Peipei Zhang, Hang Zhang, Chongyang Li, Baigao Yang, Xiaoyi Feng, Jianhua Cao, Weihua Du, Muhammad Shahzad, Adnan Khan, Shao-Chen Sun, Xueming Zhao
The improvement of in vitro embryo development is a gateway to enhance the output of assisted reproductive technologies. The Wnt and Hippo signaling pathways are crucial for the early development of bovine embryos. This study investigated the development of bovine embryos under the influence of a Hippo signaling agonist (LPA) and a Wnt signaling inhibitor (DKK1). In this current study, embryos produced in vitro were cultured in media supplemented with LPA and DKK1. We comprehensively analyzed the impact of LPA and DKK1 on various developmental parameters of the bovine embryo, such as blastocyst formation, differential cell counts, YAP fluorescence intensity and apoptosis rate...
March 31, 2024: International Journal of Molecular Sciences