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Hon Yan Kelvin Yip, Chin Wee Tan, Yumiko Hirokawa, Antony Wilks Burgess
Although small intestinal epithelial stem cells form crypts when using intestinal culture conditions, colon stem cells usually form colonospheres. Colon mesenchymal cell feeder layers can stimulate colon crypts to form organoids and produce crypts. We have investigated whether conditioned medium from colon mesenchymal cells can also stimulate colonosphere and organoid cryptogenesis. We prepared conditioned medium (CM) from WEHI-YH2 cells (mouse colon myofibroblasts); the CM stimulated both colonosphere formation and organoid cryptogenesis in vitro...
2018: PloS One
Daniel Niederer, Tobias Engeroff, Jan Wilke, Lutz Vogt, Winfried Banzer
OBJECTIVES: A media-based collection and further analysis of relative return to play (RTP) rates and the corresponding quality of play after anterior cruciate ligament (ACL) rupture in top level football was the aim of our study. METHODS: In the 5-year case-control study, male players from the first two leagues of the five European countries top leagues, who sustained a total ACL rupture during the season 2010/11 and/or 2011/12, were included. For them and a matched control sample (ratio 1:2), data were retrieved from the publicly available and validated media-based platforms (transfermarkt...
June 21, 2018: Scandinavian Journal of Medicine & Science in Sports
Bahaa Alshawaf, Hans-Peter Weber, Matthew Finkelman, Khaled El Rafie, Yukio Kudara, Panos Papaspyridakos
OBJECTIVES: The aim of this in vitro study was to compare the accuracy of printed implant casts from digital impressions with two intra-oral scanners (IOS) to stone casts from conventional impressions. The hypothesis was that printed casts would be more accurate than stone casts from conventional impressions. MATERIALS AND METHODS: A mandibular stone cast with Kennedy class II edentulism was fabricated using two internal connection tissue-level implants at 30 degrees to each other (Replace Select RP, Nobel Biocare) to serve as master...
June 21, 2018: Clinical Oral Implants Research
Michal Wilk, Artur Golas, Petr Stastny, Monika Nawrocka, Michal Krzysztofik, Adam Zajac
Volume and intensity of exercise are the basic components of training loads, having a direct impact on adaptive patterns. Exercise volume during resistance training has been conventionally evaluated as a total number of repetitions performed in each set, regardless of the time and speed of performing individual exercises. The aim of this study was to evaluate the effect of varied tempos i.e. regular (REG) 2/0/2/0, medium (MED) 5/0/3/0 and slow (SLO) 6/0/4/0 during resistance exercise on training volume, based on the total number of performed repetitions (REPsum1-5 ) and time under tension (TUTsum1-5 )...
June 2018: Journal of Human Kinetics
Shahla Momeni Danaei, Shabnam Ajami, Hamideh Etemadi, Niloofar Azadeh
Background: Literature is controversial in regard with alterations in pharyngeal airway dimensions subsequent to maxillary protraction. The correlation between maxillary protraction and sagittal airway dimension was investigated in association with tongue and soft palate position in skeletal Class III children. The results were compared with those of an untreated Class III and a Class I malocclusion control group. Materials and Methods: In this cross-sectional study pre- and post-treatment cephalometric radiographs of 19 Class III patients (6 males, 13 females; mean age, 7...
May 2018: Dental Research Journal
Claudio Poggio, Matteo Viola, Maria Mirando, Marco Chiesa, Riccardo Beltrami, Marco Colombo
Background: Acidic beverages, such as soft drinks (orange juice and cola), can produce erosion of resin composites. The aim of this in vitro study was to evaluate the effect of immersion in acidic drink on the Vickers microhardness (VK) of different esthetic restorative materials (one nanohybrid Ormocer-based composite, one nanoceramic composite, one nanofilled composite, and one microfilled hybrid composite). Materials and Methods: In this in vitro study, thirty specimens of each esthetic restorative material were divided into three subgroups ( n = 10): specimens of group 1 were used as control, specimens of group 2 were immersed in 50 ml of acidic drink for 1 day, specimens of group 3 were immersed in 50 ml of acidic drink for 7 days...
May 2018: Dental Research Journal
Juan Sebastián Celis, Daniel Wibberg, Catalina Ramírez-Portilla, Oliver Rupp, Alexander Sczyrba, Anika Winkler, Jörn Kalinowski, Thomas Wilke
Background: Many cnidarians, including stony corals, engage in complex symbiotic associations, comprising the eukaryotic host, photosynthetic algae, and highly diverse microbial communities-together referred to as holobiont. This taxonomic complexity makes sequencing and assembling coral host genomes extremely challenging. Therefore, previous cnidarian genomic projects were based on symbiont-free tissue samples. However, this approach may not be applicable to the majority of cnidarian species for ecological reasons...
June 18, 2018: GigaScience
Jonathan M Davis, Jeffrey Shenberger, Norma Terrin, Janis L Breeze, Mark Hudak, Elisha M Wachman, Peter Marro, Erica L Oliveira, Karen Harvey-Wilkes, Adam Czynski, Barbara Engelhardt, Karen D'Apolito, Debra Bogen, Barry Lester
Importance: Although opioids are used to treat neonatal abstinence syndrome (NAS), the best pharmacologic treatment has not been established. Objective: To compare the safety and efficacy of methadone and morphine in NAS. Design, Setting, and Participants: In this randomized, double-blind, intention-to-treat trial, term infants from 8 US newborn units whose mothers received buprenorphine, methadone, or opioids for pain control during pregnancy were eligible...
June 18, 2018: JAMA Pediatrics
Sezen Çimen Polat, Celal Bulgay, İmdat Yarım, Halil İbrahim Cicioğlu, Ebru Çetin
The purpose of this study is to examine the correlation between leg power and balance performance in elite wrestlers and injury history. In the research group, there are 18 elite freestyle male wrestlers at the ages of 24.27 ± 3.18 years, with a height of 171.86 ± 5.44 cm and a body weight of 79.27 ± 11.16 kg. Information on the injury history of the athletes’ upper legs for the past year was collected via interviews with the club’s physiotherapist. Laboratory tests to measure performance assessed height, body weight, Y balance and isokinetic leg strength...
April 17, 2018: Sports
Artur Golas, Adam Maszczyk, Petr Stastny, Michal Wilk, Krzysztof Ficek, Robert George Lockie, Adam Zajac
BACKGROUND: The bench press (BP) is a complex exercise demanding high neuromuscular activity. Therefore, the main objective of this study was to identify the patterns of muscular activity of the prime movers on both sides of an elite powerlifter. METHODS: A World Champion (RAW PR 320 kg) participated in the study (age: 34 years; body mass: 103 kg; body height 1.72 m; one-repetition maximum (1 RM) flat bench press: 220 kg). The subject performed one repetition of the flat bench press with: 70% 1 RM (150 kg) and 90% 1 RM (200 kg) in tempos: 2 s eccentric and 1 s concentric phase; 6 s eccentric and 1 s concentric phase)...
March 28, 2018: Sports
Eszter Szabo, Reka Mizsei, Piotr Wilk, Zsofia Zambo, Beata Torocsik, Manfred S Weiss, Vera Adam-Vizi, Attila Ambrus
We report the crystal structures of the human (dihydro)lipoamide dehydrogenase (hLADH, hE3) and its disease-causing homodimer interface mutant D444V-hE3 at 2.27 and 1.84Å resolution, respectively. The wild type structure is a unique uncomplexed, unliganded hE3 structure with the true canonical sequence. Based on the structural information a novel molecular pathomechanism is proposed for the impaired catalytic activity and enhanced capacity for reactive oxygen species generation of the pathogenic mutant. The mechanistic model involves a previously much ignored solvent accessible channel leading to the active site that might be perturbed also by other disease-causing homodimer interface substitutions of this enzyme...
June 13, 2018: Free Radical Biology & Medicine
Lucia Wilke, Francisca E Abellan Schneyder, Markus Roskopf, Andreas C Jenke, Andreas Heusch, Kai O Hensel
Speckle tracking echocardiography (STE) is increasingly used during functional assessments. However, reproducibility and dependence on echocardiographic image quality for speckle tracking stress echocardiography in pediatric patients have not been studied to date. 127 consecutive normotensive children without structural heart disease (mean age 13.4 ± 3.0 years, 50.4% female) underwent a stepwise semisupine cycle ergometric protocol. Left ventricular (LV) myocardial peak strain and strain rate were assessed at rest and during exercise...
June 15, 2018: Scientific Reports
C Kachulis, K Abe, C Bronner, Y Hayato, M Ikeda, K Iyogi, J Kameda, Y Kato, Y Kishimoto, Ll Marti, M Miura, S Moriyama, M Nakahata, Y Nakano, S Nakayama, Y Okajima, A Orii, G Pronost, H Sekiya, M Shiozawa, Y Sonoda, A Takeda, A Takenaka, H Tanaka, S Tasaka, T Tomura, R Akutsu, T Kajita, K Kaneyuki, Y Nishimura, K Okumura, K M Tsui, L Labarga, P Fernandez, F D M Blaszczyk, J Gustafson, E Kearns, J L Raaf, J L Stone, L R Sulak, S Berkman, S Tobayama, M Goldhaber, M Elnimr, W R Kropp, S Mine, S Locke, P Weatherly, M B Smy, H W Sobel, V Takhistov, K S Ganezer, J Hill, J Y Kim, I T Lim, R G Park, A Himmel, Z Li, E O'Sullivan, K Scholberg, C W Walter, T Ishizuka, T Nakamura, J S Jang, K Choi, J G Learned, S Matsuno, S N Smith, J Amey, R P Litchfield, W Y Ma, Y Uchida, M O Wascko, S Cao, M Friend, T Hasegawa, T Ishida, T Ishii, T Kobayashi, T Nakadaira, K Nakamura, Y Oyama, K Sakashita, T Sekiguchi, T Tsukamoto, K E Abe, M Hasegawa, A T Suzuki, Y Takeuchi, T Yano, T Hayashino, T Hiraki, S Hirota, K Huang, M Jiang, K E Nakamura, T Nakaya, B Quilain, N D Patel, R A Wendell, L H V Anthony, N McCauley, A Pritchard, Y Fukuda, Y Itow, M Murase, F Muto, P Mijakowski, K Frankiewicz, C K Jung, X Li, J L Palomino, G Santucci, C Vilela, M J Wilking, C Yanagisawa, S Ito, D Fukuda, H Ishino, A Kibayashi, Y Koshio, H Nagata, M Sakuda, C Xu, Y Kuno, D Wark, F Di Lodovico, B Richards, R Tacik, S B Kim, A Cole, L Thompson, H Okazawa, Y Choi, K Ito, K Nishijima, M Koshiba, Y Totsuka, Y Suda, M Yokoyama, R G Calland, M Hartz, K Martens, C Simpson, Y Suzuki, M R Vagins, D Hamabe, M Kuze, T Yoshida, M Ishitsuka, J F Martin, C M Nantais, H A Tanaka, A Konaka, S Chen, L Wan, Y Zhang, R J Wilkes, A Minamino
A search for boosted dark matter using 161.9 kt yr of Super-Kamiokande IV data is presented. We search for an excess of elastically scattered electrons above the atmospheric neutrino background, with a visible energy between 100 MeV and 1 TeV, pointing back to the Galactic center or the Sun. No such excess is observed. Limits on boosted dark matter event rates in multiple angular cones around the Galactic center and Sun are calculated. Limits are also calculated for a baseline model of boosted dark matter produced from cold dark matter annihilation or decay...
June 1, 2018: Physical Review Letters
Eleonora Sementino, Craig W Menges, Yuwaraj Kadariya, Suraj Peri, Jinfei Xu, Zemin Liu, Richard G Wilkes, Kathy Q Cai, Frank J Rauscher, Andres J Klein-Szanto, Joseph R Testa
Malignant mesothelioma (MM) is a therapy-resistant cancer arising primarily from the lining of the pleural and peritoneal cavities. The most frequently altered genes in human MM are cyclin-dependent kinase inhibitor 2A (CDKN2A), which encodes components of the p53 (p14ARF) and RB (p16INK4A) pathways, BRCA1-associated protein 1 (BAP1), and neurofibromatosis 2 (NF2). Furthermore, the p53 gene (TP53) itself is mutated in ~15% of MMs. In many MMs, the PI3K-PTEN-AKT-mTOR signaling node is hyperactivated, which contributes to tumor cell survival and therapeutic resistance...
June 15, 2018: Journal of Cellular Physiology
Patrik Nordeman, Ulrika Yngve, Helena Wilking, Sven Åke Gustavsson, Jonas Eriksson, Gunnar Antoni
The radiosynthesis and GMP validation of [11 C] AMT for human use is described. Three consecutive batches were produced giving 940-3790 MBq (4-17% RCY, decay corrected, based on [11 C]CO2 ). The molar activity at the end of synthesis was 19-35 GBq/μmol, the radiochemical purity was ≥98% and the enantiomeric purity was >99%. While the synthesis method was automated using a new generation of synthesis equipment, Tracer Production System (TPS) developed in house, the method should be readily applicable to other synthesis platforms with minor modifications...
June 14, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
Benjamin Wilke, Anna Cooper, C Parker Gibbs, Andre Spiguel
Allograft reconstruction of large segmental intercalary bone defects after tumor resection is a well-accepted surgical technique. Although results generally have been satisfactory, nonunion at the allograft-host bone junction site remains a notable concern. Various reports have described attempts to enhance junctional healing with a variety of complex osteotomies, often complicating an already complex procedure. The use of an innovative reverse reaming technique can decrease the level of intraoperative difficulty mating the allograft-host junction and theoretically may improve junctional healing by enhancing stability and increasing the contact surface area...
June 11, 2018: Journal of the American Academy of Orthopaedic Surgeons
Maria C Spriggs, Richard W Gerhold, Rebecca P Wilkes, Paul Keenlance, Robert L Sanders, Jill Witt, Eric Clark, Debra Miller
  To better understand the clinical pathology, diseases, and causes of mortality of reintroduced American martens ( Martes americana) in Michigan, a study was conducted from 2011 to 2015 in the Upper and Lower Peninsulas of Michigan. Samples obtained from live trapping ( n = 58) or harvested carcasses ( n = 34) were serologically tested for select pathogens. Antibodies against Toxoplasma gondii and canine distemper virus were detected in 58 and 3.4% of samples, respectively. All samples were seronegative for Leptospira spp...
June 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Coleen Crouch, Justin M Curley, Jamie T Carreno, Joshua E Wilk
Introduction: While combat readiness is a top priority for the U.S. Army, there is concern that behavioral health (BH) return to duty (RTD) practices may under-represent the number of soldiers available for deployment. Profiling, the official administrative process by which medical duty limitations are communicated to commanders, was recently found to be significantly under-reporting BH readiness levels in one Army Division. This is a safety issue in addition to a readiness problem, and underscores the importance of better understanding RTD practices in order to offer solutions...
June 11, 2018: Military Medicine
Yalda Baradaran-Heravi, Lubina Dillen, Hung Phuoc Nguyen, Sara Van Mossevelde, Jonathan Baets, Peter De Jonghe, Sebastiaan Engelborghs, Peter P De Deyn, Mathieu Vandenbulcke, Rik Vandenberghe, Philip Van Damme, Patrick Cras, Eric Salmon, Matthis Synofzik, Peter Heutink, Carlo Wilke, Javier Simon-Sanchez, Ricard Rojas-Garcia, Janina Turon-Sans, Alberto Lleó, Ignacio Illán-Gala, Jordi Clarimón, Barbara Borroni, Alessandro Padovani, Pau Pastor, Monica Diez-Fairen, Miquel Aguilar, Ellen Gelpi, Raquel Sanchez-Valle, Sergi Borrego-Ecija, Radoslav Matej, Eva Parobkova, Benedetta Nacmias, Sandro Sorbi, Silvia Bagnoli, Alexandre de Mendonça, Catarina Ferreira, Matthew J Fraidakis, Janine Diehl-Schmid, Panagiotis Alexopoulos, Maria Rosário Almeida, Isabel Santana, Christine Van Broeckhoven, Julie van der Zee
We evaluated the genetic contribution of the T cell-restricted intracellular antigen-1 gene (TIA1) in a European cohort of frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS) patients. Exonic resequencing of TIA1 in 1120 patients (693 FTD, 341 ALS, 86 FTD-ALS) and 1039 controls identified in total 5 rare heterozygous missense variants, affecting the TIA1 low-complexity domain (LCD). Only 1 missense variant, p.Met290Thr, identified in a familial FTD patient with disease onset at 64 years, was absent from controls yet received a combined annotation-dependent depletion score of 11...
May 23, 2018: Neurobiology of Aging
Vrushali V Agashe, Ewa Jankowska-Gan, Melissa Keller, Jeremy A Sullivan, Lynn D Haynes, John F Kernien, Jose R Torrealba, Drew Roenneburg, Melanie Dart, Marco Colonna, David S Wilkes, William J Burlingham
Leukocyte-associated Ig-like receptor 1 (LAIR1) is an ITIM-bearing collagen receptor expressed by leukocytes and is implicated in immune suppression. However, using a divalent soluble LAIR1/Fc recombinant protein to block interaction of cell surface LAIR1 with matrix collagen, we found that whereas Th 1 responses were enhanced as predicted, Th 17 responses were strongly inhibited. Indeed, LAIR1 on both T cells and monocytes was required for optimal Th 17 responses to collagen type (Col)V. For pre-existing "natural" Th 17 response to ColV, the LAIR1 requirement was absolute, whereas adaptive Th 17 and Th 1/17 immune responses in both mice and humans were profoundly reduced in the absence of LAIR1...
June 8, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
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