Biao Yuan, Athina G Portaliou, Rinky Parakra, Jochem H Smit, Jiri Wald, Yichen Li, Bindu Srinivasu, Maria S Loos, Harveer Singh Dhupar, Dirk Fahrenkamp, Charalampos G Kalodimos, Franck Duong van Hoa, Thorben Cordes, Spyridoula Karamanou, Thomas C Marlovits, Anastassios Economou
Type III protein secretion is widespread in Gram-negative pathogens. It comprises the injectisome with a surface-exposed needle and an inner membrane translocase. The translocase contains the SctRSTU export channel enveloped by the export gate subunit SctV that binds chaperone/exported clients and forms a putative ante-chamber. We probed the assembly, function, structure and dynamics of SctV from enteropathogenic E.coli (EPEC). In both EPEC and E.coli lab strains, SctV forms peripheral oligomeric clusters that are detergent-extracted as homo-nonamers...
August 26, 2021: Journal of Molecular Biology