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https://www.readbyqxmd.com/read/28432940/a-sandwich-immunoassay-for-brucellosis-diagnosis-based-on-immune-magnetic-beads-and-quantum-dots
#1
Li Li, Dehui Yin, Kun Xu, Yushen Liu, Dandan Song, Juan Wang, Chao Zhao, Xiuling Song, Juan Li
Brucellosis is a serious zoonosis with a rapid increase in incidence across epidemic regions. Currently, there are numerous methods for diagnosing brucellosis. However, these studies often have a few defects, such as low sensitivity, poor specificity, time consuming, laborious, and even potential biological risk. To overcome these shortcomings, we have developed a rapid, sensitive and accurate diagnosis procedure for brucellosis based on the immune magnetic beads (IMB) probe and quantum dots (QDs) - staphylococcal protein A (SPA) probe...
April 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28411163/development-of-a-multi-epitope-peptide-vaccine-inducing-robust-t-cell-responses-against-brucellosis-using-immunoinformatics-based-approaches
#2
Mahdiye Saadi, Ahmad Karkhah, Hamid Reza Nouri
Current investigations have demonstrated that a multi-epitope peptide vaccine targeting multiple antigens could be considered as an ideal approach for prevention and treatment of brucellosis. According to the latest findings, the most effective immunogenic antigens of brucella to induce immune responses are included Omp31, BP26, BLS, DnaK and L7-L12. Therefore, in the present study, an in silico approach was used to design a novel multi-epitope vaccine to elicit a desirable immune response against brucellosis...
April 11, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28389465/acquired-brucella-bacteraemia-in-a-young-infant
#3
Ambar Bhatnagar, Daisy Khera, Kuldeep Singh, Anuradha Sharma
An infant aged 6 weeks presented with failure to thrive since birth, fever and lethargy for 4 days. The child was fed on unpasteurised diluted goat's milk after birth. She was diagnosed to have brucellosis both by blood culture and serology. Her mother's Brucella serology was negative. She was successfully treated with injection amikacin and tablet rifampicin and had satisfactory weight gain thereafter. Acquired brucellosis should be considered in the differential diagnosis of failure to thrive in young infants...
April 7, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28388689/whole-genome-sequencing-of-brucella-melitensis-isolated-from-57-patients-in-germany-reveals-high-diversity-in-strains-from-middle-east
#4
Enrico Georgi, Mathias C Walter, Marie-Theres Pfalzgraf, Bernd H Northoff, Lesca M Holdt, Holger C Scholz, Lothar Zoeller, Sabine Zange, Markus H Antwerpen
Brucellosis, a worldwide common bacterial zoonotic disease, has become quite rare in Northern and Western Europe. However, since 2014 a significant increase of imported infections caused by Brucella (B.) melitensis has been noticed in Germany. Patients predominantly originated from Middle East including Turkey and Syria. These circumstances afforded an opportunity to gain insights into the population structure of Brucella strains. Brucella-isolates from 57 patients were recovered between January 2014 and June 2016 with culture confirmed brucellosis by the National Consultant Laboratory for Brucella...
2017: PloS One
https://www.readbyqxmd.com/read/28388625/poor-performance-of-the-rapid-test-for-human-brucellosis-in-health-facilities-in-kenya
#5
William A de Glanville, Raquel Conde-Álvarez, Ignacio Moriyón, John Njeru, Ramón Díaz, Elizabeth A J Cook, Matilda Morin, Barend M de C Bronsvoort, Lian F Thomas, Samuel Kariuki, Eric M Fèvre
Human brucellosis is considered to be an important but typically under-diagnosed cause of febrile illness in many low and middle-income countries. In Kenya, and throughout East Africa, laboratory diagnosis for the disease is based primarily on the febrile antigen Brucella agglutination test (FBAT), yet few studies of the diagnostic accuracy of this test exist. Assessment of the performance of the FBAT is essential for its appropriate clinical use, as well as for evaluating surveillance data reported by public health systems...
April 7, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28386600/novel-solutions-for-vaccines-and-diagnostics-to-combat-brucellosis
#6
Satadru Sekhar Mandal, Lucy Duncombe, N Vijaya Ganesh, Susmita Sarkar, Laurence Howells, Philip J Hogarth, David R Bundle, John McGiven
Brucellosis is diagnosed by detection of antibodies in the blood of animals and humans that are specific for two carbohydrate antigens, termed A and M, which are present concurrently in a single cell wall O-polysaccharide. Animal brucellosis vaccines contain these antigenic determinants, and consequently infected and vaccinated animals cannot be differentiated as both groups produce A and M specific antibodies. We hypothesized that chemical synthesis of a pure A vaccine would offer unique identification of infected animals by a synthetic M diagnostic antigen that would not react with antibodies generated by this vaccine...
March 22, 2017: ACS Central Science
https://www.readbyqxmd.com/read/28386446/early-animal-farming-and-zoonotic-disease-dynamics-modelling-brucellosis-transmission-in-neolithic-goat-populations
#7
Guillaume Fournié, Dirk U Pfeiffer, Robin Bendrey
Zoonotic pathogens are frequently hypothesized as emerging with the origins of farming, but evidence of this is elusive in the archaeological records. To explore the potential impact of animal domestication on zoonotic disease dynamics and human infection risk, we developed a model simulating the transmission of Brucella melitensis within early domestic goat populations. The model was informed by archaeological data describing goat populations in Neolithic settlements in the Fertile Crescent, and used to assess the potential of these populations to sustain the circulation of Brucella...
February 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28384918/an-uncommon-presentation-of-brucella-endocarditis-masquerading-as-neurobrucellosis
#8
Satish Venkata Pendela, Neha Agrawal, Thomas Mathew, Sudha Vidyasagar, Pujitha Kudaravalli
Brucella endocarditis is a rare but a severe complication of brucellosis, observed in less than 2% of cases. It is the main cause responsible for up to 80% of deaths in brucellosis. Herein, we present a case of brucella endocarditis that developed on a native aortic valve, but presented to us with fever for several months and acute neurological symptoms. This case report signifies the importance of considering brucella endocarditis as one of the differentials in patients presenting with Pyrexia of Unknown Origin (PUO) and Central Nervous System (CNS) manifestations...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28384245/imported-human-brucellosis-in-belgium-bio-and-molecular-typing-of-bacterial-isolates-1996-2015
#9
Delphine Hanot Mambres, Samira Boarbi, Patrick Michel, Nora Bouker, Luisa Escobar-Calle, Damien Desqueper, Tiziano Fancello, Marjan Van Esbroeck, Jacques Godfroid, David Fretin, Marcella Mori
OBJECTIVES: The aim of this study was to characterize by classical biotyping and Multi-Locus variable number tandem repeats (VNTR) Analysis (MLVA) all Brucella spp. derived from human cases in Belgium from 1996 to 2015. Final goals were to determine the species and biovar, to trace-back on genetic grounds the origin of each strain when patient history and risk factors were missing, and to survey for particular trends at the national level. METHODS: A total of 37 Brucella strains, isolated from 37 patients in Belgium, were analyzed by both classical biotyping and MLVA, and the genetic patterns compared to those of human strains isolated worldwide...
2017: PloS One
https://www.readbyqxmd.com/read/28382472/omp31-plays-an-important-role-on-outer-membrane-properties-and-intracellular-survival-of-brucella-melitensis-in-murine-macrophages-and-hela-cells
#10
L Verdiguel-Fernández, R Oropeza-Navarro, Francisco J Basurto-Alcántara, A Castañeda-Ramírez, Antonio Verdugo-Rodríguez
Brucellosis is an infectious disease that affects practically all species of mammals, including human, and is a major zoonosis worldwide. Brucella spp. are facultative intracellular pathogens that have the ability to survive and multiply in phagocytic and nonphagocytic cells such as trophoblast and epithelial cells. Among the six recognized species of the genus Brucella, Brucella melitensis is the main etiological agent involved in goat brucellosis and is also the most pathogenic for human. It causes significant losses in livestock production as a result of abortions, metritis, infertility, and birth of weak animals...
April 5, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28381135/detection-of-brucella-canis-infection-in-dogs-by-blood-culture-and-bacterial-identification-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#11
Tanya J Purvis, Donna Krouse, Dawn Miller, Julia Livengood, Nagaraja R Thirumalapura, Deepanker Tewari
Brucella canis was recovered from dogs that were canine brucellosis suspect by blood culture using a modified lysis method. Organism identity was established by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The instrument-provided security library identified the isolates as Brucella species. The isolates were further identified as B. canis with the help of phenotypic and genotypic characteristics. The mass spectral profiles from characterized B. canis isolates, when added to the MALDI-TOF MS standard reference library, allowed successful presumptive identification of B...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28381128/bovine-leukemia-virus-seroprevalence-among-cattle-presented-for-slaughter-in-the-united-states
#12
Fernando V Bauermann, Julia F Ridpath, David A Dargatz
Infection with bovine leukemia virus (BLV) results in economic loss because of reduced productivity, especially reduced milk production, and early culling. In the United States, studies in 1996, 1999, and 2007 showed BLV infection to be widespread, especially in dairy herds. We updated information herein on BLV seroprevalence in the United States, using samples submitted for testing and found negative for antibodies for Brucella by the Kentucky Eastern Regional Federal Brucellosis Laboratory. From October 2014 through August 2015, 2,000 samples from all regions of the contiguous United States were selected and tested for BLV antibodies by enzyme-linked immunosorbent assay...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28376905/characterization-of-brucella-abortus-mutant-strain-%C3%AE-22915-a-potential-vaccine-candidate
#13
Yanqing Bao, Mingxing Tian, Peng Li, Jiameng Liu, Chan Ding, Shengqing Yu
Brucellosis, caused by Brucella spp., is an important zoonosis worldwide. Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted...
April 4, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28367469/evaluation-of-regulatory-t-cells-in-patients-with-acute-and-chronic-brucellosis
#14
Ali Ganji, Ghasem Mosayebi, Ehsanollah Ghaznavi-Rad, Khadije Khosravi, Nader Zarinfar
BACKGROUND: Brucellosis is one of the most common chronic diseases, with widespread distribution. In spite of cell-mediated immunity (CMI) modulated mainly via activated T-helper type 1 (Th1) cells, brucellosis can advance to chronic disease in about 10-30% of cases. Regulatory T cells (Treg cells) are involved the immune response to brucellosis; however, their role, particularly in the change from the acute to the chronic phase, have not yet been elucidated. The main hypothesis of this study was that Treg cells play critical roles in the progression of brucellosis from the acute to the chronic phase...
April 2017: Reports of Biochemistry & Molecular Biology
https://www.readbyqxmd.com/read/28348782/an-unusual-case-of-seronegative-16s-pcr-positive-brucella-infection
#15
Binutha Bharathan, Lucy Backhouse, David Rawat, Sandhia Naik, Michael Millar
INTRODUCTION: Brucella is a zoonotic infection commonly diagnosed by isolation of the organism from blood culture or positive serological testing. It is an uncommon cause of a pyrexia of unknown origin in the United Kingdom. CASE PRESENTATION: We describe the case of a 14-year-old girl with no history of travel who presented with pyrexia, weight loss, arthralgia, multiple splenic abscesses and a subsequent pleural effusion, the latter of which isolated a Brucella species on 16S rRNA PCR...
October 2016: JMM Case Reports
https://www.readbyqxmd.com/read/28340558/a-novel-real-time-pcr-assay-for-specific-detection-of-brucella-melitensis
#16
Rene Kaden, Sevinc Ferrari, Erik Alm, Tara Wahab
BACKGROUND: Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for Brucella melitensis. Whilst culture of Brucella species can be used as a diagnostic tool, real-time PCR approaches provide a much faster result...
March 24, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28337351/a-rare-form-of-brucella-bursitis-with-negative-serology-a-case-report-and-literature-review
#17
F M Almajid
Brucellosis is still endemic in certain parts of the world including the Mediterranean, the Middle East, Latin America, and African regions. Osteoarticular manifestations are common presenting features. Brucellosis presenting as prepatellar bursitis has already been reported. We present a case of seronegative olecranon bursitis with positive blood and aspirate cultures. The patient improved remarkably by treatment with streptomycin and doxycycline with no evidence or relapse.
2017: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/28333986/building-the-road-to-a-regional-zoonoses-strategy-a-survey-of-zoonoses-programmes-in-the-americas
#18
Melody J Maxwell, Mary H Freire de Carvalho, Armando E Hoet, Marco A N Vigilato, Julio C Pompei, Ottorino Cosivi, Victor J Del Rio Vilas
BACKGROUND: In recent years, global public health security has been threatened by zoonotic disease emergence as exemplified by outbreaks of H5N1 and H1N1 influenza, SARS, and most recently Ebola and Zika. Additionally, endemic zoonoses, such as rabies, burden countries year after year, placing demands on limited finances and personnel. To survey the baseline status of the emerging and endemic zoonoses programmes of the Latin American and the Caribbean (LAC) countries, the Pan American Health Organization (PAHO) conducted a survey of priority emerging and endemic zoonoses, countries´ prioritization criteria and methodologies, and suggestions to strengthen countries capacities and regional approaches to zoonoses control...
2017: PloS One
https://www.readbyqxmd.com/read/28325071/a-rare-case-of-anterior-mediastinal-mass-caused-by-brucella-infection
#19
Feridoun Sabzi, Reza Faraji
A previously healthy man, who had undergone coronary artery bypass 10 years earlier and had been diagnosed with brucellosis due to Brucella septicemia after Brucella arthritis, presented with chest pain and high fever. Anti- Brucella antibiotics were started, but after 4 weeks, his high fever remained. An infected mass was confirmed by computed tomography, and surgical intervention was performed via a median sternotomy. A large amount of thick pus gushed from an abscess in the upper mediastinum. The abscess cavity had a thick granulation wall, and cultured pus was positive for Brucella only...
March 2017: Asian Cardiovascular & Thoracic Annals
https://www.readbyqxmd.com/read/28320594/epidemiological-survey-of-human-brucellosis-in-inner-mongolia-china-2010-2014-a-high-risk-groups-based-survey
#20
Cao Ning, Guo Shuyi, Yan Tao, Zhu Hao, Xingguang Zhang
Brucellosis, a zoonosis which can seriously harm public health. Inner Mongolia is known as an endemic region of human brucellosis in China. The aim of this study is to present the epidemic of human brucellosis in Inner Mongolia from 2010 to 2014, and to provide the evidence of preventing from brucellosis in endemic areas. The study included 838,956 participants by cluster random sampling from 2010 to 2014. The contents of this study included questionnaire survey and serological testing. The seropositive rate was calculated based on the serological testing results, tested to determine whether the participants were infected by brucellosis...
March 17, 2017: Journal of Infection and Public Health
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