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https://www.readbyqxmd.com/read/29338693/in-vitro-antimicrobial-susceptibility-testing-of-human-brucella-melitensis-isolates-from-ulanqab-of-inner-mongolia-china
#1
Zhi-Guo Liu, Dong-Dong Di, Miao Wang, Ri-Hong Liu, Hong-Yan Zhao, Dong-Ri Piao, Zhong-Zhi Zhao, Yong-Qing Hao, Ya-Nan Du, Hai Jiang, Bu-Yun Cui, Xian-Zhu Xia
BACKGROUND: Brucellosis is an endemic disease in the Inner Mongolia Autonomous Region of China and Ulanqab exhibits the highest prevalence of brucellosis in this region. Due to the complex nature of Brucellosis, a cure for this disease has proven to be elusive. Furthermore, the reduced susceptibility of Brucella spp. to antimicrobial agents has been reported as a potential cause of therapeutic failure. However, detailed in vitro antimicrobial susceptibility patterns pertaining to Brucella isolates from this region have not yet been published...
January 16, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29332311/otolaryngology-head-and-neck-region-manifestations-of-brucella
#2
Anna Lazutkin, Maya Korem, Jeffrey M Weinberger, Ron Eliashar, Nir Hirshoren
OBJECTIVES/HYPOTHESIS: Patients with Brucella infection present with nonspecific symptoms originating from different organs. In this study, we investigated the manifestations involving principally the otolaryngology/head and neck region. STUDY DESIGN: Retrospective cohort chart review. METHODS: A retrospective analysis of patients diagnosed with brucellosis in a tertiary medical center. Medical records of 55 patients treated for positive Brucella blood cultures between 2007 and 2016 were analyzed...
January 14, 2018: Laryngoscope
https://www.readbyqxmd.com/read/29331367/molecular-characterization-and-differential-expression-analysis-of-interleukin-1%C3%AE-from-ovis-aries
#3
Yi-Ming Shui, Shi-Ying Lu, Xing Guo, Xi-Lin Liu, Bao-Quan Fu, Pan Hu, Lin-Lin Qu, Nan-Nan Liu, Yan-Song Li, Lu-Lu Wang, Fei-Fei Zhai, Dan-Di Ju, Zeng-Shan Liu, Yu Zhou, Hong-Lin Ren
The interleukin-1 family is an important component of the innate immune system and plays an important role in regulating immune responses on the invasion of intracellular parasites in the acquired immune system. Interleukin 1β (IL-1β) is one of the members of the IL-1 family that predominantly activates downstream signaling pathways to play immunological functions of stimulating T and B lymphocyte activation and promoting the various syntheses of inflammatory substances in conjunction with other cytokines...
January 10, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29329166/hematologic-manifestations-of-brucellosis-in-children
#4
Naphtali Justman, Yariv Fruchtman, David Greenberg, Ben-Shimol Shalom
BACKGROUND: Brucellosis is a common zoonosis in the Bedouin population of southern Israel. Limited data exist for the rate and risk factors of hematologic complication of brucellosis in children. We assessed anemia, leukopenia, thrombocytopenia and pancytopenia in childhood brucellosis in southern Israel. METHODS: Our medical center is the sole hospital in southern-Israel. All medical files of brucellosis, 2005-2014, identified through positive blood cultures or International Classification of Diseases 9 revision (ICD9) coding with positive serology, were reviewed retrospectively...
January 11, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29326214/whole-genome-sequences-of-brucella-melitensis-strain-qh61-isolated-from-yak-in-qinghai-china
#5
Xiaoan Cao, Zhaocai Li, Zhongzi Lou, Baoquan Fu, Yongsheng Liu, Youjun Shang, Jizhang Zhou, Zhizhong Jing
The facultative intracellular Gram-negative bacterium Brucella melitensis causes brucellosis in domestic and wild mammals. Brucella melitensis QH61 was isolated from a yak suffering from abortion in 2015 in Qinghai, China. Here, we report the whole-genome sequence of B. melitensis strain QH61.
January 11, 2018: Genome Announcements
https://www.readbyqxmd.com/read/29325516/brucellosis-remains-a-neglected-disease-in-the-developing-world-a-call-for-interdisciplinary-action
#6
K A Franc, R C Krecek, B N Häsler, A M Arenas-Gamboa
BACKGROUND: Brucellosis is an endemic zoonotic disease in most of the developing world that causes devastating losses to the livestock industry and small-scale livestock holders. Infected animals exhibit clinical signs that are of economic significance to stakeholders and include reduced fertility, abortion, poor weight gain, lost draught power, and a substantial decline in milk production. In humans, brucellosis typically manifests as a variety of non-specific clinical signs. Chronicity and recurring febrile conditions, as well as devastating complications in pregnant women are common sequelae...
January 11, 2018: BMC Public Health
https://www.readbyqxmd.com/read/29319583/the-infectious-and-non-infectious-etiology-clinical-picture-and-outcome-of-neutropenia-in-immunocompetent-hospitalized-children
#7
Odeya David, Yariv Fruchtman, Ruslan Sergienko, Joseph Kapelushnik, Eugene Leibovitz
BACKGROUND: Acquired neutropenia in immunocompetent children is common and its differential diagnosis ranges from benign causes to life-threatening diseases. We described the etiology, clinical picture and outcome of new onset neutropenia in immunocompetent children assessed in the emergency department and hospitalized at our medical center. PATIENTS AND METHODS: Previously healthy children admitted with neutropenia (absolute neutrophil count [ANC] <1.5 x 10/L) were included...
January 9, 2018: Pediatric Infectious Disease Journal
https://www.readbyqxmd.com/read/29310845/serological-study-of-brucellosis-in-argentine-creole-sheep
#8
Gustavo E López, Sabrina Peña, Gabriela I Escobar, Déborah B Hasan, Nidia E Lucero
Ovine cattle was introduced into America during the Spanish conquest with the second journey of Columbus to the Antilles and was disseminated throughout the region. In 1587, sheep were introduced into Argentina, later developing into the "Creole" breed. We selected 486 animals from different Argentine provinces with the aim of determining the serological status of brucellosis caused by Brucella melitensis and Brucella ovis. For the detection of antibodies against smooth Brucella spp., the Rose Bengal test (RBT) was performed as screening test while the serum agglutination test (SAT) and 2 mercapto-ethanol (2ME) were run as a confirmatory technique...
January 5, 2018: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29305897/cloning-expression-and-purification-of-virb10-protein-of-brucella-melitensis-and-to-evaluate-its-role-as-a-serological-marker-for-brucella-infection-in-experimental-and-natural-hosts
#9
Prachi Pathak, Ashu Kumar, Prabhu Prasad Sarangi, Sameer Bhagyawant, Duraipandian Thavaselvam
Brucellosis is a zoonotic disease caused by various species of the genus Brucella. The control of disease mainly depends on its accurate and early diagnosis. Culture methods employed for diagnosis are time consuming and require well equipped biosafety level 3 laboratories and hence serological tests are favored alternative for brucellosis diagnosis. At present serological diagnosis is based on LPS (lipopolysaccharide) which is less specific as the LPS antigen of Brucella species shows cross reactivity with other gram-negative bacteria...
January 3, 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29302824/brucella-abortus-phosphoglyceromutase-and-dihydrodipicolinate-reductase-induce-th1-and-th2-related-immune-responses
#10
Zhiqiang Li, Hui Zhang, Jinliang Zhang, Li Xi, Guangli Yang, Shuli Wang, Qingfeng Zhou, Xiaogen Zhang, Junbo Zhang
Brucellae are intracellular bacterial pathogens that cause Brucellosis, bringing great economic burdens to developing countries. The pathogenic mechanisms of Brucella are still poorly understood. Earlier immune response plays an important role in the Brucella infection. Phosphoglyceromutase (PGM) and dihydrodipicolinate reductase (DapB) were cloned, expressed, purified, and their immunocompetence was analyzed. Cytokines were detected by murine macrophages (RAW 264.7) and splenocytes that stimulated with the two recombinant proteins...
January 4, 2018: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/29302776/epidemiological-situation-of-transboundary-animal-diseases-in-north-african-countries-proposition-of-a-regional-control-strategy
#11
REVIEW
Moustafa Kardjadj
The Food and Agricultural Organization (FAO) defined transboundary animal diseases (TADs) as those that are of significant economic, trade, and food security importance for a considerable number of countries. TADs can easily spread to other countries, reach epidemic proportions, and where control, management, or exclusion is required cooperation between several countries. The North African countries are vulnerable to several TADs by virtue of its geographical location, its borders with the Sahel region, and peculiar control constraints on the budgets of the national veterinary services of each country and on the livelihoods of livestock owners across the region...
January 5, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29294525/-a-case-report-on-occupational-acute-mild-organophosphorus-insecticides-poisoning-caused-by-brucellosis-outbreak-disposal
#12
(no author information available yet)
No abstract text is available yet for this article.
September 20, 2017: Chinese Journal of Industrial Hygiene and Occupational Diseases
https://www.readbyqxmd.com/read/29290297/evaluation-of-three-serological-tests-for-diagnosis-of-bovine-brucellosis-in-smallholder-farms-in-pakistan-by-estimating-sensitivity-and-specificity-using-bayesian-latent-class-analysis
#13
Shumaila Arif, Jane Heller, Marta Hernandez-Jover, David M McGill, Peter C Thomson
The present study aimed to estimate the herd-level sensitivity (Se) and specificity (Sp) of three commonly used serological tests in naturally-infected cattle and buffalo in smallholder farms in Pakistan. Between February and June 2015, a cross-sectional study was carried out in five districts of Punjab (Kasur, Okara, Pakpattan, Jhelum, and Bhakkar) and two districts of Sindh (Badin and Thatta). Serum samples from mixed farms of cattle (n=441) and buffalo (n=621) were collected and tested using the Rose Bengal test (RBT), indirect ELISA (I-ELISA) and competitive ELISA (C-ELISA)...
January 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29287984/alternative-strategies-for-vaccination-to-brucellosis
#14
David W Pascual, Xinghong Yang, Hongbin Wang, Zakia Goodwin, Carol Hoffman, Beata Clapp
Brucellosis remains burdensome for livestock and humans worldwide. Better vaccines for protection are needed to reduce disease incidence. Immunity to brucellosis and barriers to protection are discussed. The benefits and limitations of conventional and experimental brucellosis vaccines are outlined, and novel vaccination strategies needed to ultimately protect against brucellosis are introduced.
December 26, 2017: Microbes and Infection
https://www.readbyqxmd.com/read/29287761/brucellosis-in-west-and-central-africa-a-review-of-the-current-situation-in-a-changing-landscape-of-dairy-cattle-systems
#15
REVIEW
L Craighead, A Meyer, B Chengat, I Musallam, J Akakpo, P Kone, J Guitian, B Häsler
Brucellosis is a neglected endemic zoonosis in West and Central Africa. In this narrative review, evidence of livestock and human infection is presented along with details of past and current control strategies in 14 selected countries. Data from available literature is combined with expert opinion elicited during a regional workshop on brucellosis diagnostics. Demographic changes that affect both the epidemiology of brucellosis and the success of control or surveillance are also considered. The evidence suggests that brucellosis prevalence in emerging peri-urban dairy cattle systems may be higher than that found in traditional transhumant extensive systems...
December 26, 2017: Acta Tropica
https://www.readbyqxmd.com/read/29278754/genotyping-of-brucella-melitensis-and-brucella-abortus-strains-in-kazakhstan-using-mlva-15
#16
Аida Daugaliyeva, Akhmetzhan Sultanov, Bekbolat Usserbayev, Sholpan Baramova, Paola Modesto, Akmaral Adambayeva, Pier Luigi Acutis, Simone Peletto
Currently, although the prevalence of brucellosis in Kazakhstan remains high, there are limited data available on the genetic diversity of circulating Brucella strains. Here, MLVA was employed to genotype a panel of 102 Brucella isolates collected from eight Kazakh regions and neighboring countries (Russia, Kyrgyzstan) during the period 1935-2017. MLVA-11 analysis classified 64 B. abortus strains into genotypes 72, 82, 331, 71, 341 and 69, while one genotype was novel, having no correspondence within the MLVA international database...
December 23, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/29277251/-characterization-of-the-genetic-variability-of-field-strains-of-brucella-canis-isolated-in-antioquia
#17
Juana L Vidal Arboleda, Luisa F Ortiz Roman, Martha Olivera Angel
Brucella canis is a facultative intracellular pathogen responsible for canine brucellosis, a zoonotic disease that affects canines, causing abortions and reproductive failure; and the production of non-specific symptoms in humans. In 2005 the presence of B. canis in Antioquia was demonstrated and the strains were identified as type 2. The sequencing of the genome of a field strain denoted Brucella canis str. Oliveri, showed species-specific indel events, which led us to investigate the genomic characteristics of the B...
December 22, 2017: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/29274056/documenting-the-absence-of-brucellosis-in-cattle-goats-and-dogs-in-a-one-health-interface-in-the-mnisi-community-limpopo-south-africa
#18
Gregory Simpson, Tanguy Marcotty, Elodie Rouille, Nelson Matekwe, Jean-Jacques Letesson, Jacques Godfroid
This study shows the absence of the world's most common bacterial zoonoses caused by Brucella abortus and Brucella melitensis in cattle, goats and dogs in an agro-pastoral community in South Africa, where heifer vaccination against brucellosis with the live Strain 19 vaccine is compulsory. The study site is bordering wildlife reserves with multiple wildlife species infected with brucellosis. The results showed a low seroprevalence (1.4%) in cattle. Seroprevalence in cattle decreased with age after 4 years in females, males were less positive than females and a tissue culture from a brucellin skin test-positive male was negative...
December 23, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29241098/under-ascertainment-under-reporting-and-timeliness-of-iranian-communicable-disease-surveillance-system-for-zoonotic-diseases
#19
P A Kazerooni, M Fararouei, M Nejat, M Akbarpoor, Z Sedaghat
OBJECTIVES: The important steps in controlling infectious diseases are fast detection, proper treatment and on-time reporting of cases to the appropriate authorities. This study was conducted to evaluate the quality of Iranian communicable diseases surveillance system (CDSS) for zoonotic diseases in rural areas of Fars province (Iran's third largest province). STUDY DESIGN: The three most important evaluation indices of CDSS, namely under-ascertainment, under-reporting and timeliness, for the three most common zoonotic diseases were measured using independent data source obtained from door-to-door interviews and patients' medical records...
December 11, 2017: Public Health
https://www.readbyqxmd.com/read/29238329/relative-quantitative-proteomic-analysis-of-brucella-abortus-reveals-metabolic-adaptation-to-multiple-environmental-stresses
#20
Xiaodong Zai, Qiaoling Yang, Ying Yin, Ruihua Li, Mengying Qian, Taoran Zhao, Yaohui Li, Jun Zhang, Ling Fu, Junjie Xu, Wei Chen
Brucella spp. are facultative intracellular pathogens that cause chronic brucellosis in humans and animals. The virulence of Brucella primarily depends on its successful survival and replication in host cells. During invasion of the host tissue, Brucella is simultaneously subjected to a variety of harsh conditions, including nutrient limitation, low pH, antimicrobial defenses, and extreme levels of reactive oxygen species (ROS) via the host immune response. This suggests that Brucella may be able to regulate its metabolic adaptation in response to the distinct stresses encountered during its intracellular infection of the host...
2017: Frontiers in Microbiology
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