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https://www.readbyqxmd.com/read/28738473/-hoof-genotyping-characteristics-of-brucella-melitensis-strains-isolated-in-ulanqab-of-inner-mongolia-autonomous-region
#1
Z G Liu, M Wang, R H Liu, X J Shang, B Y Cui
Objective: To investigate the HOOF genotyping characteristics of 83 Brucella (B.) melitensis strains isolated in Ulanqab of Inner Mongolia Autonomous Region from 2012 to 2015. Methods: A total of 83 B. melitensis strains were detected by convention identification and AMOS-PCR, then HOOF protocol with eight VNTR locus were used for the genotyping of the strains, and the allelic diversity of each VNTR locus and the discriminatory power of VNTR typing of HOOF were assessed by Hunter-Gaston Discriminatory index...
July 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://www.readbyqxmd.com/read/28737147/brucella-causing-liver-abscess-in-a-child-with-selective-iga-deficiency
#2
Devdeep Mukherjee, Priyankar Pal, Ritabrata Kundu
BACKGROUND: Brucella has been known to cause pyrexia of unknown origin. CASE CHARACTERISTICS: 9-year-old boy with fever and abdominal pain; multiple abscesses within the liver on ultrasonography. OBSERVATIONS: IgM Antibodies against Brucella were raised in his serum sample, and Brucella serum agglutination test was positive. Immunological work-up suggested selective IgA deficiency. Reduction in size following treatment with trimethoprim-sulphamethoxazole, amikacin and doxycycline...
July 15, 2017: Indian Pediatrics
https://www.readbyqxmd.com/read/28735383/brucellosis-causes-alteration-in-trace-elements-and-oxidative-stress-factors
#3
Naser Zanganeh, Ehsan Siahpoushi, Nejat Kheiripour, Sima Kazemi, Mohammad Taghi Goodarzi, Mohammad Yousef Alikhani
Brucellosis is regarded as one of the most common diseases among humans and livestock. In the present study, we aimed to assess the effect of this disease on the level of various cations including copper (Cu), manganese (Mn), zinc (Zn), and magnesium (Mg) as well as oxidative stress status in the serum of people suffering from brucellosis. The present case-study was carried out on 40 patients with brucellosis (case) and 20 healthy people (control). Blood specimens were taken from all the people and the level of essential trace elements and oxidative stress status were measured...
July 22, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28733024/ecohealth-research-in-africa-where-from-where-to
#4
EDITORIAL
Robert Bergquist, Norbert W Brattig, Moses J Chimbari, Jakob Zinsstag, Jürg Utzinger
Epidemiological mapping and risk profiling build on the idea that diseases are tied to social-ecological systems that govern the distribution and abundance of transmissible pathogens, vectors and hosts. This is the heart of the emerging field of ecohealth, which examines how biological, cultural, demographic, economic, physical, political and social environments change can affect the health and wellbeing of humans, animals and ecosystems and the services they provide. This paper is an overview of a special issue of Acta Tropica, whose 15 publications reflect a geographically and epidemiologically diverse landscape of ecohealth in Africa...
July 18, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28719613/visual-detection-of-brucella-in-bovine-biological-samples-using-dna-activated-gold-nanoparticles
#5
Dheeraj Pal, Nongthombam Boby, Satish Kumar, Gurpreet Kaur, Syed Atif Ali, Julien Reboud, Sameer Shrivastava, Praveen K Gupta, Jonathan M Cooper, Pallab Chaudhuri
Brucellosis is a bacterial disease, which, although affecting cattle primarily, has been associated with human infections, making its detection an important challenge. The existing gold standard diagnosis relies on the culture of bacteria which is a lengthy and costly process, taking up to 45 days. New technologies based on molecular diagnosis have been proposed, either through dip-stick, immunological assays, which have limited specificity, or using nucleic acid tests, which enable to identify the pathogen, but are impractical for use in the field, where most of the reservoir cases are located...
2017: PloS One
https://www.readbyqxmd.com/read/28717311/using-real-time-polymerase-chain-reaction-as-an-alternative-rapid-method-for-enumeration-of-colony-count-in-live-brucella-vaccines
#6
Waleed S Shell, Mahmoud L Sayed, A A Samy, Ghada Mohamed Al-Sadek, Gina Mohamed Mohamed Abd El-Hamid, Abdel Hakam M Ali
AIM: Brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. It has economic, public health, and bio-risk importance. Control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. There are three types of animal Brucella live vaccines - Brucella melitensis Rev-1 vaccine, Brucella abortus S19, and B. abortus RB51. Evaluation of these vaccines depends mainly on enumeration of Brucella viable count...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28713359/exploring-the-diversity-of-field-strains-of-brucella-abortus-biovar-3-isolated-in-west-africa
#7
Moussa Sanogo, David Fretin, Eric Thys, Claude Saegerman
Brucellosis is one of the most widespread bacterial zoonotic diseases in the world, affecting both humans and domestic and wild animals. Identification and biotyping of field strains of Brucella are of key importance for a better knowledge of the epidemiology of brucellosis, for identifying appropriate antigens, for managing disease outbreaks and for setting up efficient preventive and control programmes. Such data are required both at national and regional level to assess potential threats for public health...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28705257/single-stage-transforaminal-decompression-debridement-interbody-fusion-and-posterior-instrumentation-for-lumbosacral-brucellosis
#8
Yakefu Abulizi, Wei-Dong Liang, Aikeremujiang Muheremu, Maierdan Maimaiti, Wei-Bin Sheng
BACKGROUND: Spinal brucellosis is a less commonly reported infectious spinal pathology. There are few reports regarding the surgical treatment of spinal brucellosis in existing literature. This retrospective study was conducted to determine the effectiveness of single-stage transforaminal decompression, debridement, interbody fusion, and posterior instrumentation for lumbosacral spinal brucellosis. METHODS: From February 2012 to April 2015, 32 consecutive patients (19 males and 13 females, mean age 53...
July 14, 2017: BMC Surgery
https://www.readbyqxmd.com/read/28702153/neurobrucellosis-in-systemic-lupus-erythematosus
#9
Jamshid Vafaeimanesh, Alireza Shahamzeh, Mohammad Bagherzadeh
BACKGROUND: Brucellosis is a zoonotic infection which is endemic in many countries. It is a multisystem disease which may present with a broad spectrum of clinical manifestations and complications. Neurobrucellosis is an uncommon complication of brucellosis. CASE PRESENTATION: A 25-year-old woman with a history of lupus for 5 months referred to the emergency ward of Shahid Beheshti Hospital of Qom due to vertigo, drop attack and a convulsion episode from the previous day...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/28702152/brucella-pericarditis-a-forgotten-cause-of-chest-pain
#10
Feridoun Sabzi, Reza Faraji
BACKGROUND: Brucellosis pericarditis is an extremely rare involvement of the cardiovascular system with Brucella species that has worldwide distribution and is endemic in many provinces of Iran. CASE PRESENTATION: The present report describes an exceptional case of pericardial involvement by Brucella melitensis in a 50- year-old woman. The patient presented with night sweats, mild fever, weight loss, and chest pain. CONCLUSION: Pericarditis occurs during the course of brucellosis as an isolated cardiac symptom and may be asymptomatic or may be manifested with chest pain as ischemic heart disease...
2017: Caspian Journal of Internal Medicine
https://www.readbyqxmd.com/read/28699872/in-vitro-synergistic-effects-of-a-short-cationic-peptide-and-clinically-used-antibiotics-against-drug-resistant-isolates-of-brucella-melitensis
#11
Zohreh Mohammadi Azad, Hoda Moravej, Mahdi Fasihi-Ramandi, Faramarz Masjedian, Raziyeh Nazari, Reza Mirnejad, Mehrdad Moosazadeh Moghaddam
PURPOSE: In the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. Accordingly, the aim of the current study was to evaluate antimicrobial effects of the CM11 peptide alone and combined with common antibiotics against drug-resistant isolates of Brucella melitensis. METHODOLOGY: A total of 50 pathogenic samples of B. melitensis were isolated from patients and their antibiotic susceptibility pattern was evaluated by E-test...
July 12, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28698730/neurobrucellosis-presenting-with-unilateral-abducens-nerve-palsy
#12
Mohsen Andisheh, Susan Amirsalari, Mohammad Torkaman, Marzieh Sabzechian, Shahla Afsharpaiman
One of the rare complications of brucellosis is neurobrucellosis. There have been numerous reports showing clinical forms of brucellosis affecting CNS, such as cranial nerve involvement, myelitis, vascular disease, radiculoneuritis, meningitis, meningoencephalitis, and demyelinating disease. In this case report, we introduce a 2.5 yr old girl with unilateral abducens nerve palsy referred to Baghiyatallah Hospital Outpatient Clinic, Tehran, Iran in June 2015.
2017: Iranian Journal of Child Neurology
https://www.readbyqxmd.com/read/28693438/evaluation-of-recombinant-porin-romp2a-protein-as-a-potential-antigen-candidate-for-serodiagnosis-of-human-brucellosis
#13
Prachi Pathak, Ashu Kumar, Duraipandian Thavaselvam
BACKGROUND: Brucellosis is an important zoonotic disease caused by different Brucella species and human brucellosis is commonly prevalent in different states of India. Among various Brucella species, B. melitensis is most pathogenic to human and included as category B biothreat which can cause infection through aerosol, cut, wounds in skin and contact with infected animals. The diagnosis of human brucellosis is very important for proper treatment and management of disease as there is no vaccine available for human use...
July 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28693306/tannic-acid-mediated-immune-activation-attenuates-brucella-abortus-infection-in-mice
#14
Alisha Wehdnesday Bernardo Reyes, Huynh Tan Hop, Lauren Togonon Arayan, Tran Xuan Ngoc Huy, Wongi Min, Hu Jang Lee, Hong Hee Chang, Suk Kim
Brucellosis is an emerging infectious disease affecting humans and animals. In this study, we investigated the in vitro and in vivo effects of tannic acid (TA) against Brucella (B.) abortus infection. Reduced F-actin polymerization and MAPKs (ERK 1/2 and p38α) phosphorylation were observed in TA-treated cells as compared with control cells after infection. The mice were infected via intraperitoneal route and were orally given with TA or PBS for 14 days. The weights of the spleens from TA-treated and control mice were not different, however the splenic proliferation of B...
July 10, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28692674/human-brucellosis-in-portugal-retrospective-analysis-of-suspected-clinical-cases-of-infection-from-2009-to-2016
#15
Ana Pelerito, Rita Cordeiro, Rita Matos, Maria Augusta Santos, Sofia Soeiro, João Santos, Carla Manita, Carla Rio, M Santo, Eleonora Paixão, Alexandra Nunes, Sofia Núncio
Brucellosis is a zoonosis that is emerging in some regions of the world. Although brucellosis is a disease of obligatory declaration and is not eradicated in Portugal, no prevalence data is available in this country. In this study, we retrospectively analyzed the data available at the Reference Laboratory at the Portuguese National Institute of Health during the past 7 years (2009-2016) in order to get insight into the epidemiological scenario of brucellosis in Portugal. A total of 2313 biological samples from patients with clinical suspicion of brucellosis were subjected to immunological techniques for laboratory diagnosis...
2017: PloS One
https://www.readbyqxmd.com/read/28689480/prevalence-and-risk-factors-of-brucellosis-among-veterinary-health-care-professionals
#16
Rajeswari Shome, Triveni Kalleshamurthy, Padmashree B Shankaranarayana, Prashanth Giribattanvar, Nagarathna Chandrashekar, Nagalingam Mohandoss, Bibek Ranjan Shome, Ashok Kumar, Sukhadeo B Barbuddhe, Habibur Rahman
The study describes prevalence, clinical symptoms and risk factors for brucellosis in personnel engaged in veterinary health care in Karnataka, India. A total of 1050 sera samples were collected from animal handlers, veterinarians, veterinary students, para-veterinarians and persons engaged in artificial insemination of animals. The sera samples were tested for brucellosis by Rose Bengal plate test (RBPT), serum agglutination test (SAT), IgG and IgM indirect ELISA and PCR. Age, sex, clinical symptoms and risk factors were recorded in structured questionnaire...
July 8, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28670862/temporal-analysis-and-costs-of-ruminant-brucellosis-control-programme-in-egypt-between-1999-and-2011
#17
M M Eltholth, Y M Hegazy, W F El-Tras, M Bruce, J Rushton
Data for the prevalence of brucellosis in ruminants in Egypt are scarce; recent studies suggest the disease is endemic, with a high prevalence. The aim of this study was to assess the financial costs and the impact of the current control programme on the pattern of brucellosis among ruminants between 1999 and 2011. A univariate binary logistic regression model was used to compare between seropositive proportions for different years for each species. The proportion of seropositive cattle was significantly increased from 2000 to 2004 then significantly decreased from 2005 to 2011...
August 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28670646/serological-survey-of-toxoplasmosis-neosporosis-and-brucellosis-among-cattle-herds-in-oyo-state-south-western-nigeria
#18
Adekunle Ayinmode, Victor Akinseye, Gereon Schares, Simeon Cadmus
BACKGROUND: Several zoonotic diseases are known to constitute great impediment to livestock management and production worldwide, especially in developing countries where control measures are largely non-existent. This study sets out to investigate the occurrence of toxoplasmosis, neosporosis and brucellosis among cattle herds in Oyo State, southwest Nigeria. MATERIALS AND METHODS: A cross-sectional survey to screen for antibodies to Toxoplasma gondii, Neospora caninum and Brucella abortus was conducted among 174 cattle in 17 herds...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28669636/occurrence-of-oxidative-stress-in-dairy-cows-seropositives-for-brucella-abortus
#19
Géssica Perin, Juscivete F Fávero, Diego R T Severo, Anielen D Silva, Gustavo Machado, Hugo L Araújo, Walter Lilenbaum, Vera M Morsch, Maria Rosa C Schetinger, Ricardo S Jordão, Lenita M Stefani, Nathieli B Bottari, Aleksandro S Da Silva
Bovine brucellosis is an important zoonotic disease caused by the bacterium Brucella abortus that leads to economic losses due to animal discard and commercial restrictions. Since positive animals for brucellosis are culled, little is known about the pathogenesis of this disease. Therefore, the aims of this study were to evaluate possible changes in the activity of deaminase adenosine (ADA) and the oxidative stress in cows seropositives for brucellosis (Experiment I), and to evaluate the seroprevalence of B...
June 29, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28666718/performance-of-microbiological-serological-molecular-and-modified-seminal-plasma-methods-in-the-diagnosis-of-brucella-abortus-in-semen-and-serum-of-bovine-bulls
#20
G Nardi Júnior, J Megid, L A Mathias, L Paulin, A F Vicente, A Cortez, F J P Listoni, G H B Lara, R G Motta, M G M Chacur, F M Monteiro, M G Ribeiro
Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA)...
June 27, 2017: Biologicals: Journal of the International Association of Biological Standardization
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