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Meki Bilici, Fikri Demir, Murat Muhtar Yılmazer, Fatma Bozkurt, Volkan Tuzcu
BACKGROUND: The clinical spectrum of Brucella infection is quite diverse and characterized by multi-system involvement. Patients present with myocarditis, endocarditis, or pericarditis. Infective endocarditis is the most common cardiovascular complication in patients with brucellosis. Although conduction abnormalities are seen in cases with endocarditis, they are reported very rarely in the setting of cardiac Brucella infection. CASE REPORT: An eight and a half-year-old male patient was referred to our clinic due to inadequate response to cotrimaxazole plus streptomycin treatment at the 15th day of admission...
September 2016: Balkan Medical Journal
Viroj Wiwanitkit
No abstract text is available yet for this article.
2016: Caspian Journal of Internal Medicine
Mohamed M El-Diasty, Heba A Ahmed, Ashraf E Sayour, Fatma I El Hofy, Asmaa B M B Tahoun, Saleh M Shafik
The objective of the present study was to estimate the seroprevalence of Brucella spp. in humans and cattle at Sharkia Governorate, Egypt. In addition, identification of Brucella spp. in milk samples by PCR and culture with the evaluation of the risk factors associated with Brucella spp. seroprevalence in humans were carried out. Overall, the seroprevalence of Brucella antibodies in the examined cattle was 23.8%, while in human participants it was 21%. The examination of 205 milk samples using PCR revealed that 6...
October 18, 2016: Vector Borne and Zoonotic Diseases
Leonardo A Gómez, Francisco I Alvarez, Pablo A Fernández, Manuel R Flores, Raúl E Molina, Roberto F Coloma, Angel A Oñate
Immunogenicity induced by recombinant plasmids based on the BAB1_0267 and BAB1_0270 open reading frames (ORFs) of Brucella abortus 2308 was evaluated. Bioinformatics analyses indicate that the BAB1_0267 and BAB1_0270 ORFs encode a protein with a SH3 domain and a Zn-dependent metalloproteinase, respectively. Both ORFs have important effects on intracellular survival and replication of B. abortus 2308, mediated via professional and non-professional phagocytic cells. Our results show that immunization with the recombinant plasmid based on the BAB1_0267 ORF significantly increases the production of IgG1, levels of IFN-γ and the lymphoproliferative response of splenocytes...
2016: Frontiers in Cellular and Infection Microbiology
Marcela Suárez-Esquivel, Nazareth Ruiz-Villalobos, Amanda Castillo-Zeledón, César Jiménez-Rojas, R Martin Roop Ii, Diego J Comerci, Elías Barquero-Calvo, Carlos Chacón-Díaz, Clayton C Caswell, Kate S Baker, Esteban Chaves-Olarte, Nicholas R Thomson, Edgardo Moreno, Jean J Letesson, Xavier De Bolle, Caterina Guzmán-Verri
Brucellosis is a bacterial infectious disease affecting a wide range of mammals and a neglected zoonosis caused by species of the genetically homogenous genus Brucella. As in most studies on bacterial diseases, research in brucellosis is carried out by using reference strains as canonical models to understand the mechanisms underlying host pathogen interactions. We performed whole genome sequencing analysis of the reference strain B. abortus 2308 routinely used in our laboratory, including manual curated annotation accessible as an editable version through a link at https://en...
2016: Frontiers in Microbiology
Pedro F Soler-Lloréns, Chris R Quance, Sara D Lawhon, Tod P Stuber, John F Edwards, Thomas A Ficht, Suelee Robbe-Austerman, David O'Callaghan, Anne Keriel
Brucella are highly infectious bacterial pathogens responsible for brucellosis, a frequent worldwide zoonosis. The Brucella genus has recently expanded from 6 to 11 species, all of which were associated with mammals; The natural host range recently expanded to amphibians after some reports of atypical strains from frogs. Here we describe the first in depth phenotypic and genetic characterization of a Brucella strains isolated from a frog. Strain B13-0095 was isolated from a Pac-Man frog (Ceratophyrus ornate) at a veterinary hospital in Texas and was initially misidentified as Ochrobactrum anthropi...
2016: Frontiers in Cellular and Infection Microbiology
K Rajkumar, A Bhattacharya, S David, S Hari Balaji, R Hariharan, M Jayakumar, N Balaji
AIM: This study was conducted to assess the extent of knowledge, awareness, attitude, and risks of zoonotic diseases among livestock owners in Puducherry region. MATERIALS AND METHODS: A total of 250 livestock farmers were selected randomly from eight revenue villages. And each farmer was interviewed with a questionnaire containing both open- and close-ended questions on various aspects of zoonotic diseases, a total of 49 questionnaires were framed to assess the source and transmission of infection to the farmers and to test their knowledge and awareness about zoonotic diseases...
September 2016: Veterinary World
A K M A Rahman, C Saegerman, D Berkvens
BACKGROUND: A Bayesian latent class evaluation was used to estimate the true prevalence of brucellosis in livestock farmers and patients with prolonged pyrexia (PP) and to validate three conditionally dependent serological tests: indirect ELISA (iELISA), Rose Bengal Test (RBT), and standard tube agglutination (STAT). A total of 335 sera from livestock farmers and 300 sera from PP patients were investigated. RESULTS: The true prevalence of brucellosis in livestock farmers and PP patients was estimated to be 1...
2016: Tropical Medicine and Health
Nawal Al Shehhi, Faisal Aziz, Farida Al Hosani, Bashir Aden, Iain Blair
BACKGROUND: Worldwide, human brucellosis remains an important and widespread infection. In the past, there were limited data on the occurrence of human brucellosis in the United Arab Emirates and the reported incidence appeared to be low compared with similar areas. In 2009, a new web-based infectious disease surveillance system was introduced in the Emirate of Abu Dhabi. This paper reports data from this new system on human brucellosis for the 6 years 2010 to 2015. METHODS: A dataset was extracted for each case of human brucellosis reported to the notification system for the 6 year period January 2010 to December 2015...
October 12, 2016: BMC Infectious Diseases
C Sandeep Reddy, K S Ashok Kumar, T Sreenivas
No abstract text is available yet for this article.
January 2016: Journal of the Association of Physicians of India
Suresh Kumar, Arun Kumar Tungaria
No abstract text is available yet for this article.
January 2016: Journal of the Association of Physicians of India
Marie J Ducrotoy, Kevin L Bardosh
Brucellosis is a major neglected zoonotic disease, whose burden both in animals and humans is severely under-reported. Diagnosis in humans identifies cases in order to treat the disease at the individual level. In animals diagnosis is implemented at the population level in the context of appropriate control or eradication strategies. Molecular and bacteriological diagnosis are rarely undertaken in sub-Saharan Africa, at least outside research projects, due to cost, skills and laboratory infrastructure issues...
October 7, 2016: Acta Tropica
Ferah Budak, Salih Haldun Bal, Gulcin Tezcan, Halis Akalın, Guher Goral, Haluk Barbaros Oral
Brucellosis is a zoonotic disease that is still endemic in developing countries. Despite early diagnosis and treatment of patients, chronic infections are seen in 10-30% of patients. In this study, we aimed to investigate the immunological factors that play roles in the transition of brucellosis from acute infection into chronic infection. Here, more than 2000 miRNAs were screened in peripheral blood mononuclear cells (PBMCs) of patients with acute or chronic brucellosis and healthy controls by using miRNA array, and the results of the miRNA array were validated through qRT-PCR...
2016: Journal of Immunology Research
A Mailles, B Garin-Bastuji, J P Lavigne, M Jay, A Sotto, M Maurin, I Pelloux, D O'Callaghan, V Mick, V Vaillant, H De Valk
: Brucellosis is a bacterial zoonotic disease mainly transmitted to humans by ruminants. In France, brucellosis has disappeared from ruminants herds. Human brucellosis surveillance is performed through mandatory notification and the national reference center. METHODS: We report the results of human brucellosis surveillance from 2004 to 2013 with regards to epidemiological, clinical and microbiological data. RESULTS: A total of 250 cases were notified, making an annual incidence of 0...
October 4, 2016: Médecine et Maladies Infectieuses
Krishna Prasad Acharya, Nirajan Niroula, Krishna Kaphle
Brucellosis is an abortifacient zoonotic disease which remains prominent in third world countries like Nepal. Brucellosis possess a public health concern, the incidences of which in livestocks can present substantial economic and health burdens for herders and health professionals. In Nepal, several cases of bovine including human brucellosis have been reported. This paper aims to evaluate the current status of the disease, the mechanism of infection, pathogenesis, zoonotic potential, diagnostic advances, treatment regimens, and the preventive measures that can be adopted in managing human brucellosis in under-developed countries like Nepal...
October 1, 2016: Epidemiology and Health
Hui Zhang, Xiaoxia Dou, Zhiqiang Li, Yu Zhang, Jing Zhang, Fei Guo, Yuanzhi Wang, Zhen Wang, Tiansen Li, Xinli Gu, Chuangfu Chen
Brucellosis is primarily a disease of domestic animals in which the bacteria localizes to fetal tissues such as embryonic trophoblast cells and fluids containing erythritol, which stimulates Brucella spp. growth. The utilization of erythritol is a characteristic of the genus Brucella. The ery operon contains four genes (eryA, eryB, eryC and eryD) for the utilization of erythritol, and plays a major role in the survival and multiplication of Brucella spp. The objective of the present study was to conduct a preliminary characterization of differential genes expression of the ery operon at several time points after Brucella infected embryonic trophoblast cells (HPT-8 cells)...
October 2016: Experimental and Therapeutic Medicine
Mohamed Barkallah, Yaakoub Gharbi, Sonia Zormati, Nesrine Karkouch, Zouhir Mallek, Michel Gautier, Radhouane Gdoura, Imen Fendri
In this study, we conducted an investigation to determine the true prevalence of bovine and ovine brucellosis in central-eastern Tunisia. A total of 1134 veterinary samples taken from 130 ruminant herds were screened for brucellosis using IS711-based real-time PCR assay. Sera collected from the ruminants were tested using the Rose Bengal test and indirect enzyme-linked immunosorbent assay. Based on serological and molecular results, the true adjusted animal population level prevalence was 23.5 % in cattle, against 13...
October 1, 2016: Tropical Animal Health and Production
Akm Anisur Rahman, Dirk Berkvens, Claude Saegerman, David Fretin, Noor Muhammad, Akram Hossain, Emmanuel Abatih
INTRODUCTION: This study describes the seroprevalence of human brucellosis among pyretic patients and detection of Brucella abortus DNA from seropositive pyretic patients using real-time polymerase chain reaction (rtPCR) for the first time in Bangladesh. METHODOLOGY: Blood samples were collected from 300 pyretic patients from October 2007 to May 2008 and subjected to three serological tests: Rose-Bengal plate test (RBT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (iELISA)...
September 30, 2016: Journal of Infection in Developing Countries
Don O'Grady, Kevin Kenny, Seamus Power, John Egan, Fergus Ryan
Intestinal infection by Yersinia enterocolitica serotype O:9 (YeO9) in cattle has been linked to false positive serological reactivity (FPSR) in diagnostic tests for brucellosis. Although eradicated in Ireland, brucellosis monitoring still identifies seropositive animals, usually one or two (termed singletons) per herd, which are classed as FPSR. To investigate a link between FPSR and YeO9, faeces and blood were collected from singleton FPSR cattle, and from companion animals, in eight selected herds with more than one FPSR animal, for YeO9 culture and Brucella serology...
October 2016: Veterinary Journal
A Praud, M Durán-Ferrer, D Fretin, M Jaÿ, M O'Connor, A Stournara, M Tittarelli, I Travassos Dias, B Garin-Bastuji
Bovine brucellosis is an infectious disease of worldwide public health and economic importance. The usual tests for the diagnosis of this disease include the Rose-Bengal test (RBT), complement fixation test (CFT), serum agglutination test (SAT) and indirect ELISA. New tests such as competitive ELISAs (C-ELISA) and fluorescence polarisation assay (FPA) have been developed. However, C-ELISA may correspond to different protocols and a wide variation may exist in their diagnostic performance. The aim of this study was to evaluate three commercially available C-ELISA kits (C-ELISA1-3) and FPA for the diagnosis of bovine brucellosis and compare test performance with RBT, CFT, indirect ELISA and FPA...
October 2016: Veterinary Journal
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