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Junbo Zhang, Shuanghong Yin, Dewu Yi, Hong Zhang, Zhiqiang Li, Fei Guo, Chuangfu Chen, Weihuan Fang, Jiafu Wang
Brucellosis is a globally distributed zoonotic disease that causes animal and human diseases. Although effective, the current Brucella vaccines (strain M5-90 or others) have several drawbacks. The first is their residual virulence for animals and humans and the second is their inability to differentiate natural infection from that caused by vaccination. In the present study, Brucella melitensis M5-90 manB mutant (M5-90ΔmanB) was generated to overcome these drawbacks. M5-90ΔmanB showed significantly reduced survival in macrophages and mice, and induced strong protective immunity in BALB/c mice...
September 12, 2017: Microbial Pathogenesis
Zhiqiang Li, Shuli Wang, Hui Zhang, Li Xi, Jinliang Zhang, Xiaogen Zhang, Jincheng Han, Junbo Zhang
It is essential to improve animal vaccine for brucellosis since conventional vaccines are residual virulent and poor protective effect, limit their applications. To solve these problems, the recombinant DNA vaccines were appeared, which could improve protective immunity and were attenuated to animals. In current research, the recombinant DNA vaccine (pVGntR) based on transcriptional regulator GntR of Brucella abortus (B. abortus) was constructed. The results show that pVGntR is significantly more protective than the conventional RB51 vaccine...
September 12, 2017: Microbial Pathogenesis
Carlo Caffarelli, Francesca Santamaria, Dora Di Mauro, Carla Mastrorilli, Silvia Montella, Sergio Bernasconi
This review reports main progresses in various pediatric issues published in Italian Journal of Pediatrics and in international journals in 2016. New insights in clinical features or complications of several disorders may be useful for our better understanding. They comprise severe asthma, changing features of lupus erythematosus from birth to adolescence, celiac disease, functional gastrointestinal disorders, Moebius syndrome, recurrent pneumonia. Risk factors for congenital heart defects, Kawasaki disease have been widely investigated...
September 16, 2017: Italian Journal of Pediatrics
Sanja Duvnjak, Silvio Špičić, Darja Kušar, Bojan Papić, Irena Reil, Maja Zdelar-Tuk, Željko Pavlinec, Martina Đuras, Tomislav Gomerčić, Rene S Hendriksen, Željko Cvetnić
Brucella spp. that cause marine brucellosis are becoming more important, as the disease appears to be more widespread than originally thought. Here, we report a whole and annotated genome sequence of Brucella ceti CRO350, a sequence type 27 strain isolated from a bottlenose dolphin carcass found in the Croatian part of the northern Adriatic Sea.
September 14, 2017: Genome Announcements
H S Zhu, L L Wang, D H Lin, R T Hong, J M Ou, W Chen, B P Wu, W L Huang, Z H Xie, G M Chen, S G Wu, Z B Xu, Y Q Deng
Objective: To analyze the epidemiological characteristics and spatial distribution of human brucellosis in Fujian province during 2011-2016, and provide evidence for the prevention and control of the disease. Methods: The surveillance data of human brucellosis in Fujian during 2011-2016 was analyzed with software R 3.3.1, ArcGIS 10.3.1, GeoDa 1.8.8 and SaTScan 9.4.3. Results: During 2011-2016, a total of 319 human brucellosis cases were reported, the incidence increased year by year (F=11.838, P=0.026) with the annual incidence of 0...
September 10, 2017: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
Liliana Cárdenas, Oscar Melo, Jordi Casal
Bovine brucellosis is endemic in Colombia, and is a mandatory notifiable disease, subjected to a control program based on four surveillance procedures: passive surveillance, test-and-remove, certification of disease-free farms, and animal movements. The objective of this study is to estimate the evolution of bovine brucellosis in Colombia over a 7-year period (2006-2012) using data from the official control program. A total of 58 epidemiologic variables were analyzed for each year at the department level. Univariate descriptive analysis and principal components analysis (PCA) were performed to ascertain the behavior of the variables...
September 13, 2017: Tropical Animal Health and Production
Fariba Keramat, Mohammad Mehdi Majzobi, Jalal Poorolajal, Zohreh Zarei Ghane, Maryam Adabi
OBJECTIVES: Brucellosis is a systemic disease with a wide spectrum of clinical manifestations. This study aimed to determine the seroprevalence of brucellosis in human immunodeficiency virus (HIV) infected patients in Hamadan Province in the west of Iran. METHODS: A total of 157 HIV-infected patients were screened through standard serological tests, including Wright's test, Coombs' Wright test, and 2-mercaptoethanol Brucella agglutination test (2ME test), blood cultures in Castaneda media, and CD4 counting...
August 2017: Osong Public Health and Research Perspectives
Jacques Godfroid
Human brucellosis remains the commonest zoonotic disease worldwide with more than 500 000 new cases annually. Understanding the biology of Brucella infections and the transmission patterns at the wildlife/livestock/human interface is of paramount importance before implementing any brucellosis control or eradication program in animals, even more so should interventions be justified within One Health. In addition to calling for transdisciplinary collaboration, One Health formally aims to conserve the environment and to promote the well-being of animals...
2017: Archives of Public Health, Archives Belges de Santé Publique
Ana Cristina Ferreira, Rogério Tenreiro, Maria Inácia Corrêa de Sá, Ricardo Dias
BACKGROUND: Swine brucellosis caused by B. suis biovar 2 is an emergent disease in domestic pigs in Europe. The emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (Sus scrofa). In Portugal and Spain, the majority of strains share specific molecular characteristics, which allowed establishing an Iberian clonal lineage. However, several strains isolated from wild boars in the North-East region of Spain are similar to strains isolated in different Central European countries...
September 12, 2017: BMC Genomics
Shahla Afrasiabian, Behzad Mohsenpour, Ebrahim Ghaderi, Sara Nadri, Katayon Hajibagheri
Brucellosis is endemic in Iran. Several studies show that brucellosis is associated with other infectious diseases. This study aimed to determine the relationship between brucellosis and Helicobacter pylori. In this case-control study, 100 patients with brucellosis as ceases and 200 participants without brucellosis as controls were evaluated. To compare the prevalence of Helicobacter pylori in the two groups, odds ratio and confidence intervals for every variable were analyzed through using logistic regression model after adjustment for confounding factors...
September 11, 2017: Japanese Journal of Infectious Diseases
Yanyan De, Congyue Dong, Yanyang Cao, Xiaolei Wang, Xiaowen Yang, Ning Wang, Cunrui Zhang, Zhen Wang, Yanli Lyu, Qingmin Wu
A transposon mutant library of B. melitensis NI including 32,640 transposon mutants was established. By sequencing the transposon insertion sites, 10,832 mutants were successfully defined for their insertion sites. Analysis of the mutants with defined transposon insertion sites (DTIS) indicated that the insertions were well spread through the two genomes. In addition, 948 genes with no detectable transposon insertions were taken as the candidate for identification of essential genes. In comparison with the Bacterial Database of Essential Genes and by using comparative genomics analysis, 183 potential essential genes of B...
September 6, 2017: Microbial Pathogenesis
María E Cortina, Analía Novak, Luciano J Melli, Sebastián Elena, Natalia Corbera, Juan E Romero, Ana M Nicola, Juan E Ugalde, Diego J Comerci, Andrés E Ciocchini
Brucellosis is a widespread zoonotic disease caused by Brucella spp. Brucella canis is the etiological agent of canine brucellosis, a disease that can lead to sterility in bitches and dogs causing important economic losses in breeding kennels. Early and accurate diagnosis of canine brucellosis is central to control the disease and lower the risk of transmission to humans. Here, we develop and validate enzyme and lateral flow immunoassays for improved serodiagnosis of canine brucellosis using as antigen the B...
September 2017: Veterinary Microbiology
M N Aznar, M Arregui, M F Humblet, L E Samartino, C Saegerman
BACKGROUND: In Argentina, vaccination with Brucella abortus Strain 19 vaccine is mandatory. The objective of the study was to develop and test a method for evaluating, in an innovative way, some farmers' and veterinarians' management practices in relation to brucellosis and to assess the vaccination campaign and coverage. The work took place in Brandsen and Navarro districts. Four questionnaires were designed (for officials from Local Sanitary Entities, vaccinators, vet practitioners and farmers)...
September 7, 2017: BMC Veterinary Research
M S Roset, T G Alefantis, V G DelVecchio, G Briones
Brucella ssp. is a facultative intracellular pathogen that causes brucellosis, a worldwide zoonosis that affects a wide range of mammals including humans. A critical step for the establishment of a successful Brucella infection is its ability to survive within macrophages. To further understand the mechanisms that Brucella utilizes to adapt to an intracellular lifestyle, a differential proteomic study was performed for the identification of intracellular modulated proteins. Our results demonstrated that at 48 hours post-infection Brucella adjusts its metabolism in order to survive intracellularly by modulating central carbon metabolism...
September 6, 2017: Scientific Reports
Won Kyong Kim, Ja Young Moon, Jeong Sang Cho, Jin Hur
Brucella species are important etiological agents of zoonotic diseases. Attenuated Salmonella strains expressing Brucella abortus BCSP31, Omp3b and superoxide dismutase proteins were tested as vaccine candidates in this study. In order to determine the optimal dose for intraperitoneal (IP) inoculation required to obtain effective protection against brucellosis, mice were immunized with various doses of a mixture of the three vaccine strains. Fifty BALB/c mice were divided into five equal groups (groups A-E)...
September 29, 2017: Pathogens and Disease
Jose María San-Miguel Ayanz, Francisco Javier Garcia-Peña, Paula García-Lunar, Luis Miguel Ortega-Mora, María José Ruano, Gema Álvarez-García, Esther Collantes-Fernández
Wildlife health is of interest for public and animal health because wild animals have been identified as important sentinels for the surveillance for zoonotic pathogens. This work investigated Brucella spp., Coxiella burnetii, and Leptospira spp. infection seroprevalence in a free-ranging red deer population. The study was conducted in a fenced reserve with controlled hunting activity in central Spain with animals that did not have any contact with livestock. Sampling was performed at two time points before and 5 years after the implementation of new management measures, including a reduction in the red deer population in the reserve...
September 5, 2017: Vector Borne and Zoonotic Diseases
D Di Sabatino, G Garofolo, A Di Provvido, K Zilli, G Foschi, E Di Giannatale, M Ciuffetelli, F De Massis
Porcine brucellosis occurs in many countries where pigs are farmed, often representing an underrated problem. B. suis biovar 2 is the most common isolate in Europe, with high prevalence reported in wild boars in which it is generally isolated in the absence of gross lesions. In the last five years, we tested for Brucella spp. 389 lymph nodes of wild boars collected during hunting seasons or during necropsy procedures. In this paper, we describe the first case of isolation of B. suis biovar 2 from a wild boar aborted foetus, and we analyse the genomic relationships with B...
September 1, 2017: Infection, Genetics and Evolution
Youssouf B Kanouté, Biégo G Gragnon, Christian Schindler, Bassirou Bonfoh, Esther Schelling
Northern Côte d'Ivoire is the main livestock breeding zone and has the highest livestock cross-border movements in Côte d'Ivoire. The aim of this study was to provide updated epidemiological data on three neglected zoonotic diseases, namely brucellosis, Q Fever and Rift Valley Fever (RVF). We conducted three-stage cross-sectional cluster surveys in livestock and humans between 2012 and 2014 in a random selection of 63 villages and a sample of 633 cattle, 622 small ruminants and 88 people. We administered questionnaires to capture risk factors and performed serological tests including the Rose Bengal Plate Test (RBPT), Brucella spp...
September 1, 2017: Acta Tropica
Maria Cryskely A Batinga, Jaíne C Dos Santos, Julia T R Lima, Maria Fernanda D Bigotto, Kerstin Muner, Thalita Faita, Rodrigo M Soares, David A V da Silva, Trícia M F S Oliveira, Helena L Ferreira, Jaqueline A Diniz, Lara B Keid
Brucella canis, a gram-negative, facultative intracellular and zoonotic bacterium causes canine brucellosis. Direct methods are the most appropriate for the detection of canine brucellosis and bacterial isolation from blood samples has been employed as gold-standard method. However, due to the delay in obtaining results and the biological risk of the bacterial culturing, the polymerase chain reaction (PCR) has been successfully used as an alternative method for the diagnosis of the infection. Sample preparation is a key step for successful PCR and protocols that provide high DNA yield and purity are recommended to ensure high diagnostic sensitivity...
August 29, 2017: Journal of Microbiological Methods
Xiaoan Cao, Zhaocai Li, Zhongzi Lou, Baoquan Fu, Yongsheng Liu, Youjun Shang, Zhizhong Jing, Jizhang Zhou
The facultative intracellular Gram-negative bacterium Brucella melitensis causes brucellosis in domestic and wild mammals, and it is a dominant pathogen responsible for human disease. This study reports the whole-genome sequencing of B. melitensis strain QY1, isolated from sheep suffering from abortion and arthritis in 2015 in Gansu, China.
August 31, 2017: Genome Announcements
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