keyword
MENU ▼
Read by QxMD icon Read
search

Rab11

keyword
https://www.readbyqxmd.com/read/27903237/leucine-rich-repeat-kinase-2-lrrk2-regulates-%C3%AE-synuclein-clearance-in-microglia
#1
Tatsunori Maekawa, Toshikuni Sasaoka, Sadahiro Azuma, Takafumi Ichikawa, Heather L Melrose, Matthew J Farrer, Fumiya Obata
BACKGROUND: α-Synuclein (αSYN) has been genetically implicated in familial and sporadic Parkinson's disease (PD), and is associated with disease susceptibility, progression and pathology. Excess amounts of αSYN are toxic to neurons. In the brain, microglial αSYN clearance is closely related to neuronal survival. Leucine-rich repeat kinase 2 (LRRK2) is the one of the other genes implicated in familial and sporadic PD. While LRRK2 is known to be expressed in microglia, its true function remains to be elucidated...
November 30, 2016: BMC Neuroscience
https://www.readbyqxmd.com/read/27875067/transiently-expressed-atg16l1-inhibits-autophagosome-biogenesis-and-aberrantly-targets-rab11-positive-recycling-endosomes
#2
Jiehua Li, Zhixia Chen, Michael T Stang, Wentao Gao
The membrane source for autophagosome biogenesis is an unsolved mystery in the study of autophagy. ATG16L1 forms a complex with ATG12-ATG5 (the ATG16L1 complex). The ATG16L1 complex is recruited to autophagic membranes to convert MAP1LC3B-I to MAP1LC3B-II. The ATG16L1 complex dissociates from the phagophore before autophagosome membrane closure. Thus, ATG16L1 can be used as an early event marker for the study of autophagosome biogenesis. We found that among 3 proteins in the ATG16L1 complex, only ATG16L1 formed puncta-like structures when transiently overexpressed...
November 22, 2016: Autophagy
https://www.readbyqxmd.com/read/27872253/gtpase-cross-talk-regulates-trappii-activation-of-rab11-homologues-during-vesicle-biogenesis
#3
Laura L Thomas, J Christopher Fromme
Rab guanosine triphosphatases (GTPases) control cellular trafficking pathways by regulating vesicle formation, transport, and tethering. Rab11 and its paralogs regulate multiple secretory and endocytic recycling pathways, yet the guanine nucleotide exchange factor (GEF) that activates Rab11 in most eukaryotic cells is unresolved. The large multisubunit transport protein particle (TRAPP) II complex has been proposed to act as a GEF for Rab11 based on genetic evidence, but conflicting biochemical experiments have created uncertainty regarding Rab11 activation...
November 21, 2016: Journal of Cell Biology
https://www.readbyqxmd.com/read/27872148/proteomics-screen-identifies-class-i-rab11-fips-as-key-regulators-of-cytokinesis
#4
Carl Laflamme, Jacob A Galan, Khaled Ben El Kadhi, Antoine Méant, Carlos Zeledon, Sébastien Carréno, Philippe P Roux, Gregory Emery
The 14-3-3 protein family orchestrates a complex network of molecular interactions that regulates various biological processes. Owing to their role in regulating the cell cycle and protein trafficking, 14-3-3 proteins are prevalent in human diseases, such as cancer, diabetes and neurodegeneration. 14-3-3 proteins are expressed in all eukaryotic cells, suggesting that they mediate their biological functions through evolutionarily conserved protein interactions. To identify these core 14-3-3 client proteins, we used an affinity-based proteomics approach to characterize and compare the human and Drosophila 14-3-3 interactome...
November 21, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27807067/characterization-of-heparan-sulfate-proteoglycan-positive-recycling-endosomes-isolated-from-glioma-cells
#5
Katarzyna A Podyma-Inoue, Takuya Moriwaki, Anupama R Rajapakshe, Kazue Terasawa, Miki Hara-Yokoyama
BACKGROUND: Heparan sulfate proteoglycans (HSPGs)-dependent endocytic events have been involved in glioma progression. Thus, comprehensive understanding of the intracellular trafficking complexes formed in presence of HSPGs would be important for development of glioma treatments. MATERIALS AND METHODS: Subcellular fractionation was used to separate vesicles containing HSPGs from the rat C6 glioma cell line. Isolated HSPG-positive vesicles were further characterized with liquid chromatography-mass spectrometry...
November 2016: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/27802168/control-of-apico-basal-epithelial-polarity-by-the-microtubule-minus-end-binding-protein-camsap3-and-spectraplakin-acf7
#6
Ivar Noordstra, Qingyang Liu, Wilco Nijenhuis, Shasha Hua, Kai Jiang, Matthijs Baars, Sanne Remmelzwaal, Maud Martin, Lukas C Kapitein, Anna Akhmanova
The microtubule cytoskeleton regulates cell polarity by spatially organizing membrane trafficking and signaling processes. In epithelial cells, microtubules form parallel arrays aligned along the apico-basal axis, and recent work has demonstrated that the members of CAMSAP/Patronin family control apical tethering of microtubule minus ends. Here, we show that in mammalian intestinal epithelial cells, the spectraplakin ACF7 (also known as MACF1) specifically binds to CAMSAP3 and is required for the apical localization of CAMSAP3-decorated microtubule minus ends...
November 15, 2016: Journal of Cell Science
https://www.readbyqxmd.com/read/27763815/diverted-recycling-shigella-subversion-of-rabs
#7
Noelia Lopez Montero, Jost Enninga
Small GTPases of the Rab protein family control intracellular vesicular trafficking to allow their communication and maintenance. It is a common strategy for intracellular bacteria to exploit these pathways to shape their respective niches for survival. The subversion of Rabs for the generation of an intracellular environment favoring the pathogen has been described almost exclusively for intracellular bacteria that reside within bacterial containing vacuoles (BCVs). However, less is known about Rab subversion for bacteria that rupture the BCV to reach the host cytoplasm...
October 20, 2016: Small GTPases
https://www.readbyqxmd.com/read/27760768/ilk-and-cytoskeletal-architecture-an-important-determinant-of-aqp2-recycling-and-subsequent-entry-into-the-exocytotic-pathway
#8
Fahmy A Mamuya, Jose Luis Cano-Peñalver, Wei U Li, Diego Rodriguez-Puyol, Manuel Rodríguez-Puyol, Dennis Brown, Sergio de Frutos, Hua Jenny Lu
Within the past decade tremendous efforts have been made to understand the mechanism behind aquaporin-2 (AQP2) water channel trafficking and recycling, in order to open a path towards effective diabetes insipidus therapeutics. A recent study has shown that Integrin-Linked Kinase (ILK) conditional-knockdown mice developed polyuria along with decreased expression of AQP2. To understand whether ILK also regulates AQP2 trafficking in kidney tubular cells, we performed in vitro analysis using LLCPK1 cells stably expressing rat AQP2 (LLC-AQP2 cells)...
October 19, 2016: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/27732852/defective-transcytosis-of-app-and-lipoproteins-in-human-ipsc-derived-neurons-with-familial-alzheimer-s-disease-mutations
#9
Grace Woodruff, Sol M Reyna, Mariah Dunlap, Rik Van Der Kant, Julia A Callender, Jessica E Young, Elizabeth A Roberts, Lawrence S B Goldstein
We investigated early phenotypes caused by familial Alzheimer's disease (fAD) mutations in isogenic human iPSC-derived neurons. Analysis of neurons carrying fAD PS1 or APP mutations introduced using genome editing technology at the endogenous loci revealed that fAD mutant neurons had previously unreported defects in the recycling state of endocytosis and soma-to-axon transcytosis of APP and lipoproteins. The endocytosis reduction could be rescued through treatment with a β-secretase inhibitor. Our data suggest that accumulation of β-CTFs of APP, but not Aβ, slow vesicle formation from an endocytic recycling compartment marked by the transcytotic GTPase Rab11...
October 11, 2016: Cell Reports
https://www.readbyqxmd.com/read/27717139/vps35-dependent-recycling-of-trem2-regulates-microglial-function
#10
Jie Yin, Xiaocui Liu, Qing He, Lujun Zhou, Zengqiang Yuan, Siqi Zhao
Triggering receptor expressed on myeloid cells 2 (Trem2), an immune-modulatory receptor, is preferentially expressed in microglia of central nervous system. Trem2 might be involved in the development of Alzheimer's disease (AD) through regulating the inflammatory responses and phagocytosis of microglia. However, the intracellular trafficking of Trem2 remains unclear. In this study, we showed that Trem2 in the plasma membrane underwent endocytosis and recycling. Trem2 is internalized in a clathrin-dependent manner and then recycled back to the plasma membrane through vacuolar protein sorting 35 (Vps35), the key component of cargo recognition core of retromer complex, but not Rab11...
September 26, 2016: Traffic
https://www.readbyqxmd.com/read/27698943/intracellular-trafficking-network-of-protein-nanocapsules-endocytosis-exocytosis-and-autophagy
#11
Jinxie Zhang, Xudong Zhang, Gan Liu, Danfeng Chang, Xin Liang, Xianbing Zhu, Wei Tao, Lin Mei
The inner membrane vesicle system is a complex transport system that includes endocytosis, exocytosis and autophagy. However, the details of the intracellular trafficking pathway of nanoparticles in cells have been poorly investigated. Here, we investigate in detail the intracellular trafficking pathway of protein nanocapsules using more than 30 Rab proteins as markers of multiple trafficking vesicles in endocytosis, exocytosis and autophagy. We observed that FITC-labeled protein nanoparticles were internalized by the cells mainly through Arf6-dependent endocytosis and Rab34-mediated micropinocytosis...
2016: Theranostics
https://www.readbyqxmd.com/read/27696383/rab11-collaborates-e-cadherin-to-promote-collective-cell-migration-and-indicates-a-poor-prognosis-in-colorectal-carcinoma
#12
Yuan-Chiang Chung, Wan-Chen Wei, Chia-Nung Hung, Jen-Fang Kuo, Chih-Ping Hsu, King-Jen Chang, Wei-Ting Chao
BACKGROUND: Collective cell migration, whereby the cell-cell contacts such as E-cadherin are maintained during migration, has only recently emerged, and its detailed mechanisms are still unclear. In this study, the role of Rab11, which functions in recycling endosomes, and its relationship to E-cadherin in colorectal carcinoma were identified, and the role of Rab11 in the collective cell migration of colon cancer cells was clarified. MATERIALS AND METHODS: A total of 107 patients with surgically resected colorectal carcinoma were enrolled in this immunohistochemical study...
December 2016: European Journal of Clinical Investigation
https://www.readbyqxmd.com/read/27687567/nuf-and-rip11-requirement-for-polarity-determinant-recycling-during-drosophila-development
#13
Francisco J Calero-Cuenca, Sol Sotillos
A tight relationship between apico-basal polarity and trafficking is essential for epithelial physiology and tissue homeostasis. Recent studies have described how some Rab GTPases, key components of the intracellular traffic machinery, contribute to the establishment of cell polarity in vertebrates. We have demonstrated a novel connection between cell polarity and trafficking: in Drosophila epithelia, the apical determinant aPKC is recycled via Rab11-Nuf-recycling endosomes to maintain cell polarity. Furthermore, the phosphorylation of Nuf by aPKC allows aPKC to control the sub-cellular localization of Nuf and its own membrane accumulation...
September 29, 2016: Small GTPases
https://www.readbyqxmd.com/read/27677270/rab11-a-vesicular-trafficking-protein-affects-endoreplication-through-ras-mediated-pathway-in-drosophila-melanogaster
#14
Praween Kumar Choubey, Jagat Kumar Roy
Rab11, a small monomeric GTPase associated with recycling endosomes, is a key molecule in the regulation of vesicular trafficking and is involved in the development and differentiation of many Drosophila tissues through interaction with diverse signaling pathways. In this study, we report for the first time that Rab11 affects endoreplication through a Ras-mediated pathway. Suppression of Rab11 activity in salivary glands, an endoreplicating tissue, leads to reduction in size of salivary glands with cells having a small nucleus...
September 27, 2016: Cell and Tissue Research
https://www.readbyqxmd.com/read/27669114/rabs-and-gaps-in-starvation-induced-autophagy
#15
Christopher A Lamb, Andrea Longatti, Sharon A Tooze
Formation of autophagosomes requires vesicular trafficking from virtually every subcellular compartment to the formation site. This traffic must be tightly regulated but also adaptable as different membrane compartments will contribute varying amounts of membrane, lipids and proteins to the forming autophagosome depending on the stimulus. In mammalian cells, efforts to understand how autophagosomes form have been focused on the role of Rab proteins in autophagy. Rab proteins provide specificity through their interaction with coat proteins, vesicle tethers and SNAREs...
October 2016: Small GTPases
https://www.readbyqxmd.com/read/27658318/rab11-and-phosphoinositides-a-synergy-of-signal-transducers-in-the-control-of-vesicular-trafficking
#16
Carlo Cosimo Campa, Emilio Hirsch
Rab11 and phosphoinositides are signal transducers able to direct the delivery of membrane components to the cell surface. Rab11 is a small GTPase that, by cycling from an active to an inactive state, controls key events of vesicular transport, while phosphoinositides are major determinants of membrane identity, modulating compartmentalized small GTPase function. By sharing common effectors, these two signal transducers synergistically direct vesicular traffic to specific intracellular membranes. This review focuses on the latest advances regarding the mechanisms that ensure the compartmentalized regulation of Rab11 function through its interaction with phosphoinositides...
September 14, 2016: Advances in Biological Regulation
https://www.readbyqxmd.com/read/27625181/mitotic-cells-form-actin-based-bridges-with-adjacent-cells-to-provide-intercellular-communication-during-rounding
#17
Tone A Fykerud, Lars M Knudsen, Max Z Totland, Vigdis Sørensen, Shiva Dahal-Koirala, Ragnhild A Lothe, Andreas Brech, Edward Leithe
In order to achieve accurate chromosome segregation, eukaryotic cells undergo a dramatic change in morphology to obtain a spherical shape during mitosis. Interphase cells communicate directly with each other by exchanging ions and small molecules via gap junctions, which have important roles in controlling cell growth and differentiation. As cells round up during mitosis, the gap junctional communication between mitotic cells and adjacent interphase cells ceases. Whether mitotic cells use alternative mechanisms for mediating direct cell-cell communication during rounding is currently unknown...
November 2016: Cell Cycle
https://www.readbyqxmd.com/read/27623148/coordinated-recruitment-of-spir-actin-nucleators-and-myosin-v-motors-to-rab11-vesicle-membranes
#18
Olena Pylypenko, Tobias Welz, Janine Tittel, Martin Kollmar, Florian Chardon, Gilles Malherbe, Sabine Weiss, Carina Ida Luise Michel, Annette Samol-Wolf, Andreas Till Grasskamp, Alistair Hume, Bruno Goud, Bruno Baron, Patrick England, Margaret A Titus, Petra Schwille, Thomas Weidemann, Anne Houdusse, Eugen Kerkhoff
There is growing evidence for a coupling of actin assembly and myosin motor activity in cells. However, mechanisms for recruitment of actin nucleators and motors on specific membrane compartments remain unclear. Here we report how Spir actin nucleators and myosin V motors coordinate their specific membrane recruitment. The myosin V globular tail domain (MyoV-GTD) interacts directly with an evolutionarily conserved Spir sequence motif. We determined crystal structures of MyoVa-GTD bound either to the Spir-2 motif or to Rab11 and show that a Spir-2:MyoVa:Rab11 complex can form...
2016: ELife
https://www.readbyqxmd.com/read/27622210/adp-ribosylation-factor-6-regulates-neuronal-migration-in-the-developing-cerebral-cortex-through-fip3-arfophilin-1-dependent-endosomal-trafficking-of-n-cadherin
#19
Yoshinobu Hara, Masahiro Fukaya, Kanehiro Hayashi, Takeshi Kawauchi, Kazunori Nakajima, Hiroyuki Sakagami
During neural development, endosomal trafficking controls cell shape and motility through the polarized transport of membrane proteins related to cell-cell and cell-extracellular matrix interactions. ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. We herein demonstrated that the knockdown of endogenous Arf6 in mouse cerebral cortices led to impaired neuronal migration in the intermediate zone and cytoplasmic retention of N-cadherin and syntaxin12 in migrating neurons...
July 2016: ENeuro
https://www.readbyqxmd.com/read/27621269/tdp-43-loss-of-function-inhibits-endosomal-trafficking-and-alters-trophic-signaling-in-neurons
#20
Benjamin M Schwenk, Hannelore Hartmann, Alperen Serdaroglu, Martin H Schludi, Daniel Hornburg, Felix Meissner, Denise Orozco, Alessio Colombo, Sabina Tahirovic, Meike Michaelsen, Franziska Schreiber, Simone Haupt, Michael Peitz, Oliver Brüstle, Clemens Küpper, Thomas Klopstock, Markus Otto, Albert C Ludolph, Thomas Arzberger, Peer-Hendrik Kuhn, Dieter Edbauer
Nuclear clearance of TDP-43 into cytoplasmic aggregates is a key driver of neurodegeneration in amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD), but the mechanisms are unclear. Here, we show that TDP-43 knockdown specifically reduces the number and motility of RAB11-positive recycling endosomes in dendrites, while TDP-43 overexpression has the opposite effect. This is associated with delayed transferrin recycling in TDP-43-knockdown neurons and decreased β2-transferrin levels in patient CSF Whole proteome quantification identified the upregulation of the ESCRT component VPS4B upon TDP-43 knockdown in neurons...
September 12, 2016: EMBO Journal
keyword
keyword
14311
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"