James R Goldenring, Lynne A Lapierre, Joseph T Roland, Elizabeth Manning, Cathy Caldwell, Honor L Glenn, Pierre-Olivier Vidalain, Frederic Tangy, Brenda G Hogue, C A M de Haan
The membrane (M) glycoprotein of coronaviruses (CoVs) serves as the nidus for virion assembly. Using a yeast two-hybrid screen, we identified the interaction of the cytosolic tail of Murine Hepatitis Virus (MHV-CoV) M protein with Myosin Vb (MYO5B) (Figure 1A). MHV-CoV M interacts specifically with the alternative splice variant of cellular MYO5B including Exon D (MYO5B+D), that also mediates interaction with cellular Rab10. When co-expressed in human lung epithelial A549 and canine kidney epithelial MDCK cells, MYO5B+D co-localized with MHV-CoV M protein, as well as with M proteins from porcine epidemic diarrhea Virus (PEDV-CoV), Middle East Respiratory Syndrome (MERS-CoV) and Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) (Figure 2)...
May 2022: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology