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Apical ezrin

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https://www.readbyqxmd.com/read/29719081/asynchronous-cdx2-expression-and-polarization-of-porcine-trophoblast-cells-reflects-a-species-specific-trophoderm-lineage-determination-progress-model
#1
Shichao Liu, Gerelchimeg Bou, Jianchao Zhao, Shimeng Guo, Jia Guo, Xiaogang Weng, Zhi Yin, Zhonghua Liu
Upregulation of Cdx2 expression in outer cells is a key event responsible for cell lineage segregation between inner cell mass (ICM) and trophoderm (TE) in mouse morula stage embryos. In TE cells, polarization can regulate Hippo and ROCK signaling to induce the nuclear location of YAP, which has been demonstrated to further induce the expression of Cdx2. However, we found that CDX2 expression could not be detected in the outer cells of porcine morula stage embryos and could be found in only some TE cells at the early blastocyst stage...
May 2, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29555369/occludin-induces-microvillus-formation-via-phosphorylation-of-ezrin-in-a-mouse-hepatic-cell-line
#2
Masaki Murata, Makoto Osanai, Akira Takasawa, Kumi Takasawa, Tomoyuki Aoyama, Yuka Kawada, Akihiro Yamamoto, Yusuke Ono, Yutaro Hiratsuka, Takashi Kojima, Norimasa Sawada
Apical and basolateral cell membranes are separated by tight junctions (TJs). Microvilli are limited to the apical cell membrane. TJs and microvilli are the landmarks for epithelial cell polarity. However, the direct relationship between TJ proteins (TJPs) and the components of microvilli remains unclear. In this study, we investigated whether occludin, which is considered to be a functional TJP, is involved in microvillus formation. In occludin knockout mouse hepatic cells (OcKO cells), the microvillus density was less than that in wild-type (WT) cells and the length of microvilli was short...
March 16, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29520890/protein-kinase-c-zeta-suppresses-low-or-high-grade-colorectal-cancer-crc-phenotypes-by-interphase-centrosome-anchoring
#3
Ravi Kiran Deevi, Arman Javadi, Jane McClements, Jekaterina Vohhodina, Kienan Savage, Maurice Bernard Loughrey, Emma Evergren, Frederick Charles Campbell
Histological grading provides prognostic stratification of colorectal cancer (CRC) by scoring heterogeneous phenotypes. Features of aggressiveness include aberrant mitotic spindle configurations, chromosomal breakage, and bizarre multicellular morphology, but pathobiology is poorly understood. Protein kinase C zeta (PKCz) controls mitotic spindle dynamics, chromosome segregation, and multicellular patterns, but its role in CRC phenotype evolution remains unclear. Here, we show that PKCz couples genome segregation to multicellular morphology through control of interphase centrosome anchoring...
April 2018: Journal of Pathology
https://www.readbyqxmd.com/read/29324356/inflammation-induced-er-stress-affects-absorptive-intestinal-epithelial-cells-function-and-integrity
#4
Sucheera Chotikatum, Hassan Y Naim, Nahed El-Najjar
Recent studies have linked impairment of intestinal epithelial function in inflammatory bowel disease to the disturbance of endoplasmic reticulum homeostasis (ER) in response to stress. Most studies are on goblet and Paneth cells, which are considered more susceptible to stress due to their role in the protection of intestinal epithelium against microbes and harmful substances. However, studies on the role of inflammation-induced ER stress in absorptive intestinal cells are scarce. In this study, we show, using Caco-2 cells as a model of intestinal epithelial barrier, that inducing ER stress using a cocktail mixture of pro-inflammatory mediators [TNFα (50ng/ml), MCP1 (50ng/ml), and IL-1β (25ng/ml)] as observed in IBD patients induces ER stress and leads to significant changes in key proteins of the apical (sucrase-isomaltase (SI), dipeptidyl-peptidase (DPPIV), and ezrin) and basolateral (E-cadherin, zonula occludens (ZO-1), and connexin-43) membranes...
February 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29218485/apical-membrane-alterations-in-non-intestinal-organs-in-microvillus-inclusion-disease
#5
Cameron Schlegel, Victoria G Weis, Byron C Knowles, Lynne A Lapierre, Martin G Martin, Paul Dickman, James R Goldenring, Mitchell D Shub
OBJECTIVES: Microvillus inclusion disease (MVID) is a severe form of neonatal diarrhea, caused mainly by mutations in MYO5B. Inactivating mutations in MYO5B causes depolarization of enterocytes in the small intestine, which gives rise to chronic, unremitting secretory diarrhea. While the pathology of the small intestine in MVID patients is well described, little is known about extraintestinal effects of MYO5B mutation. METHODS: We examined stomach, liver, pancreas, colon, and kidney in Navajo MVID patients, who share a single homozygous MYO5B-P660L (1979C>T p...
February 2018: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/29206870/loss-of-polarity-alters-proliferation-and-differentiation-in-low-grade-endometrial-cancers-by-disrupting-notch-signaling
#6
Erin Williams, Alejandro Villar-Prados, Jessica Bowser, Russell Broaddus, Andrew B Gladden
Cell adhesion and apicobasal polarity together maintain epithelial tissue organization and homeostasis. Loss of adhesion has been described as a prerequisite for the epithelial to mesenchymal transition. However, what role misregulation of apicobasal polarity promotes tumor initiation and/or early progression remains unclear. We find that human low-grade endometrial cancers are associated with disrupted localization of the apical polarity protein Par3 and Ezrin while, the adhesion molecule E-cadherin remains unchanged, accompanied by decreased Notch signaling, and altered Notch receptor localization...
2017: PloS One
https://www.readbyqxmd.com/read/28808054/mst4-kinase-phosphorylates-acap4-protein-to-orchestrate-apical-membrane-remodeling-during-gastric-acid-secretion
#7
Xiao Yuan, Phil Y Yao, Jiying Jiang, Yin Zhang, Zeqi Su, Wendy Yao, Xueying Wang, Ping Gui, McKay Mullen, Calmour Henry, Tarsha Ward, Wenwen Wang, Larry Brako, Ruijun Tian, Xuannv Zhao, Fengsong Wang, Xinwang Cao, Dongmei Wang, Xing Liu, Xia Ding, Xuebiao Yao
Digestion in the stomach depends on acidification of the lumen. Histamine-elicited acid secretion is triggered by activation of the PKA cascade, which ultimately results in the insertion of gastric H,K-ATPases into the apical plasma membranes of parietal cells. Our recent study revealed the functional role of PKA-MST4-ezrin signaling axis in histamine-elicited acid secretion. However, it remains uncharacterized how the PKA-MST4-ezrin signaling axis operates the insertion of H,K-ATPases into the apical plasma membranes of gastric parietal cells...
September 29, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28754689/ezrin-directly-interacts-with-aqp2-and-promotes-its-endocytosis
#8
Wei Li, William W Jin, Kenji Tsuji, Ying Chen, Naohiro Nomura, Limin Su, Naofumi Yui, Julian Arthur, Susanna Cotecchia, Teodor G Paunescu, Dennis Brown, Hua A J Lu
The water channel aquaporin-2 (AQP2) is a major regulator of water homeostasis in response to vasopressin (VP). Dynamic trafficking of AQP2 relies on its close interaction with trafficking machinery proteins and the actin cytoskeleton. Here, we report the identification of ezrin, an actin-binding protein from the ezrin/radixin/moesin (ERM) family as an AQP2-interacting protein. Ezrin was first detected in a co-immunoprecipitation (co-IP) complex using an anti-AQP2 antibody in a proteomic analysis. Immunofluorescence staining revealed the co-expression of ezrin and AQP2 in collecting duct principal cells, and VP treatment caused redistribution of both proteins to the apical membrane...
September 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28684865/dynamic-relocalization-of-nherf1-mediates-chemotactic-migration-of-ovarian-cancer-cells-toward-lysophosphatidic-acid-stimulation
#9
Yong-Seok Oh, Kyun Heo, Eung-Kyun Kim, Jin-Hyeok Jang, Sun Sik Bae, Jong Bae Park, Yun Hee Kim, Minseok Song, Sang Ryong Kim, Sung Ho Ryu, In-Hoo Kim, Pann-Ghill Suh
NHERF1/EBP50 (Na(+)/H(+) exchanger regulating factor 1; Ezrin-binding phosphoprotein of 50 kDa) organizes stable protein complexes beneath the apical membrane of polar epithelial cells. By contrast, in cancer cells without any fixed polarity, NHERF1 often localizes in the cytoplasm. The regulation of cytoplasmic NHERF1 and its role in cancer progression remain unclear. In this study, we found that, upon lysophosphatidic acid (LPA) stimulation, cytoplasmic NHERF1 rapidly translocated to the plasma membrane, and subsequently to cortical protrusion structures, of ovarian cancer cells...
July 7, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/28493957/ezrin-interacts-with-s100a4-via-both-its-n-and-c-terminal-domains
#10
Beáta Biri-Kovács, Bence Kiss, Henrietta Vadászi, Gergő Gógl, Gyula Pálfy, György Török, László Homolya, Andrea Bodor, László Nyitray
Ezrin belongs to the ERM (ezrin, radixin, moesin) protein family that has a role in cell morphology changes, adhesion and migration as an organizer of the cortical cytoskeleton by linking actin filaments to the apical membrane of epithelial cells. It is highly expressed in a variety of human cancers and promotes metastasis. Members of the Ca2+-binding EF-hand containing S100 proteins have similar pathological properties; they are overexpressed in cancer cells and involved in metastatic processes. In this study, using tryptophan fluorescence and stopped-flow kinetics, we show that S100A4 binds to the N-terminal ERM domain (N-ERMAD) of ezrin with a micromolar affinity...
2017: PloS One
https://www.readbyqxmd.com/read/28430576/ezrin-activation-by-lok-phosphorylation-involves-a-pip-2-dependent-wedge-mechanism
#11
Thaher Pelaseyed, Raghuvir Viswanatha, Cécile Sauvanet, Joshua J Filter, Michael L Goldberg, Anthony Bretscher
How cells specify morphologically distinct plasma membrane domains is poorly understood. Prior work has shown that restriction of microvilli to the apical aspect of epithelial cells requires the localized activation of the membrane-F-actin linking protein ezrin. Using an in vitro system, we now define a multi-step process whereby the kinase LOK specifically phosphorylates ezrin to activate it. Binding of PIP2 to ezrin induces a conformational change permitting the insertion of the LOK C-terminal domain to wedge apart the membrane and F-actin-binding domains of ezrin...
April 21, 2017: ELife
https://www.readbyqxmd.com/read/28396819/rab11-fip1-phosphorylation-by-mark2-regulates-polarity-in-mdck-cells
#12
Rebecca McRae, Lynne A Lapierre, Elizabeth H Manning, James R Goldenring
MARK2/Par1b/EMK1, a serine/threonine kinase, is required for correct apical/basolateral membrane polarization in epithelial cells. However, the specific substrates mediating MARK2 action are less well understood. We have now found that MARK2 phosphorylates Rab11-FIP1B/C at serine 234 in a consensus site similar to that previously identified in Rab11-FIP2. In MDCK cells undergoing repolarization after a calcium switch, antibodies specific for pS234-Rab11-FIP1 or pS227-Rab11-FIP2 demonstrate that the spatial and temporal activation of Rab11-FIP1 phosphorylation is distinct from that for Rab11-FIP2...
2017: Cellular Logistics
https://www.readbyqxmd.com/read/28068322/role-of-the-pdz-scaffold-protein-nherf1-ebp50-in-cancer-biology-from-signaling-regulation-to-clinical-relevance
#13
REVIEW
J Vaquero, T H Nguyen Ho-Bouldoires, A Clapéron, L Fouassier
The transmission of cellular information requires fine and subtle regulation of proteins that need to interact in a coordinated and specific way to form efficient signaling networks. The spatial and temporal coordination relies on scaffold proteins. Thanks to protein interaction domains such as PDZ domains, scaffold proteins organize multiprotein complexes enabling the proper transmission of cellular information through intracellular networks. NHERF1/EBP50 is a PDZ-scaffold protein that was initially identified as an organizer and regulator of transporters and channels at the apical side of epithelia through actin-binding ezrin-moesin-radixin proteins...
June 1, 2017: Oncogene
https://www.readbyqxmd.com/read/27825102/microvillar-injury-in-renal-tubular-epithelial-cells-induced-by-calcium-oxalate-crystal-and-the-protective-role-of-epigallocatechin-3-gallate
#14
Kedsarin Fong-Ngern, Arada Vinaiphat, Visith Thongboonkerd
Pathogenic mechanisms of kidney stone disease remained unclear. This study investigated its initial cellular/molecular mechanisms when calcium oxalate monohydrate (COM) crystal adhered to renal tubular cells. Transmission electron microscopy revealed decreased length and density of microvilli, whereas Western blot analysis showed that whole-cell ezrin (a microvillus-stabilizing protein), not β-actin, was decreased in COM-treated cells. Immunofluorescence staining, followed by laser-scanning confocal microscopy and subcellular fractionations, revealed decreases in both ezrin and F-/β-actin at apical membrane...
January 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27823775/similar-expression-pattern-of-nherf1-and-ezrin-in-papillary-but-not-in-solid-areas-of-human-serous-ovarian-carcinomas
#15
Brenda Demacopulo, Baltazar Eduardo Lema, Rómulo Luis Cabrini, Erica Lorena Kreimann
NHERF1 is an adaptor protein expressed in the apical membrane of polarized epithelia, which interacts with the EZRIN-Radixin-Moesin (ERM) family of proteins connecting signaling pathways to the cell cytoskeleton. NHERF1 and EZRIN cooperate in the maintenance of the apical microvilli in polarized epithelial cells. In several types of cancers, NHERF1 and EZRIN are displaced from the apical compartment to the cytoplasm and nuclei of cancer cells. At the present, the distribution of NHERF1 in ovarian tumors is not well known...
October 2016: Acta Histochemica
https://www.readbyqxmd.com/read/27761583/several-adaptor-proteins-promote-intracellular-localisation-of-the-transporter-mrp4-abcc4-in-platelets-and-haematopoietic-cells
#16
Yvonne Schaletzki, Marie-Luise Kromrey, Susanne Bröderdorf, Elke Hammer, Markus Grube, Paul Hagen, Sonja Sucic, Michael Freissmuth, Uwe Völker, Andreas Greinacher, Bernhard H Rauch, Heyo K Kroemer, Gabriele Jedlitschky
The multidrug resistance protein 4 (MRP4/ABCC4) has been identified as an important transporter for signalling molecules including cyclic nucleotides and several lipid mediators in platelets and may thus represent a novel target to interfere with platelet function. Besides its localisation in the plasma membrane, MRP4 has been also detected in the membrane of dense granules in resting platelets. In polarised cells it is localised at the basolateral or apical plasma membrane. To date, the mechanism of MRP4 trafficking has not been elucidated; protein interactions may regulate both the localisation and function of this transporter...
January 5, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27603725/anatomical-and-gene-expression-changes-in-the-retinal-pigmented-epithelium-atrophy-1-rpea1-mouse-a-potential-model-of-serous-retinal-detachment
#17
Gabriel Luna, Geoffrey P Lewis, Kenneth A Linberg, Bo Chang, Quiri Hu, Peter J Munson, Arvydas Maminishkis, Sheldon S Miller, Steven K Fisher
PURPOSE: The purpose of this study was to examine the rpea1 mouse whose retina spontaneously detaches from the underlying RPE as a potential model for studying the cellular effects of serous retinal detachment (SRD). METHODS: Optical coherence tomography (OCT) was performed immediately prior to euthanasia; retinal tissue was subsequently prepared for Western blotting, microarray analysis, immunocytochemistry, and light and electron microscopy (LM, EM). RESULTS: By postnatal day (P) 30, OCT, LM, and EM revealed the presence of small shallow detachments that increased in number and size over time...
September 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27581796/ezrin-and-hnrnp-expression-correlate-with-increased-virus-release-rate-and-early-onset-of-virus-induced-apoptosis-of-mdck-suspension-cells
#18
Sabine Kluge, Yvonne Genzel, Kim Laus, Anja Serve, Antje Pflugmacher, Britta Peschel, Erdmann Rapp, Udo Reichl
With the aim to adapt high-yield adherent cell lines to suspension growth, Madin Darby canine kidney (MDCK) suspension cells were developed recently that achieved comparable influenza virus yields despite an early induction of apoptosis compared to the parental adherent cell line. For both cell lines, a comprehensive study under comparable infection conditions is performed comprising information on: time course of viral infection, antiviral state of cells, virus-induced apoptosis, and virus-induced cellular protein expression for early and late infection with influenza A/PuertoRico/8/34 H1N1...
October 2016: Biotechnology Journal
https://www.readbyqxmd.com/read/27542690/role-of-cd34-family-members-in-lumen-formation-in-the-developing-kidney
#19
Zhufeng Yang, Susan E Zimmerman, Jun Tsunezumi, Caitlin Braitsch, Cary Trent, David M Bryant, Ondine Cleaver, Consuelo González-Manchón, Denise K Marciano
Previous studies have shown CD34 family member Podocalyxin is required for epithelial lumen formation in vitro. We demonstrate that Endoglycan, a CD34 family member with homology to Podocalyxin, is produced prior to lumen formation in developing nephrons. Endoglycan localizes to Rab11-containing vesicles in nephron progenitors, and then relocalizes to the apical surface as progenitors epithelialize. Once an apical/luminal surface is formed, Endoglycan (and the actin-binding protein Ezrin) localize to large, intraluminal structures that may be vesicles/exosomes...
October 1, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27401686/the-joubert-syndrome-protein-inpp5e-controls-ciliogenesis-by-regulating-phosphoinositides-at-the-apical-membrane
#20
Wenyan Xu, Miaomiao Jin, Ruikun Hu, Hong Wang, Fan Zhang, Shiaulou Yuan, Ying Cao
Phosphoinositides, a family of phosphorylated derivatives of phosphatidylinositol (PtdIns), are tightly regulated both temporally and spatially by PtdIns phosphatases and kinases. Mutations in inositol polyphosphate 5-phosphatase E (INPP5E) cause Joubert syndrome, a human disorder associated with numerous ciliopathic defects, including renal cyst formation, linking phosphoinositides to ciliopathies. However, the molecular mechanism by which INPP5E-mediated PtdIns signaling regulates ciliogenesis and cystogenesis is unclear...
January 2017: Journal of the American Society of Nephrology: JASN
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